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ES2143051T3 - Deteccion de patogenos fungicos usando la reaccion en cadena de la polimerasa. - Google Patents

Deteccion de patogenos fungicos usando la reaccion en cadena de la polimerasa.

Info

Publication number
ES2143051T3
ES2143051T3 ES95918255T ES95918255T ES2143051T3 ES 2143051 T3 ES2143051 T3 ES 2143051T3 ES 95918255 T ES95918255 T ES 95918255T ES 95918255 T ES95918255 T ES 95918255T ES 2143051 T3 ES2143051 T3 ES 2143051T3
Authority
ES
Spain
Prior art keywords
detection
chain reaction
polymerase chain
fungal pathogens
sequences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95918255T
Other languages
English (en)
Inventor
James M Ligon
James J Beck
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis AG
Original Assignee
Novartis AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis AG filed Critical Novartis AG
Application granted granted Critical
Publication of ES2143051T3 publication Critical patent/ES2143051T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

SE DESCRIBEN SECUENCIAS DE DNA PROCEDENTES DEL ESPACIADOR TRANSCRITO INTERNO DE LA REGION GENICA CORRESPONDIENTE AL RNA RIBOSOMICO, PARA DIFERENTES ESPECIES Y CEPAS DE SEPTORIA, PSEUDOCERCOPORELLA, FUSARIUM, Y MICOSPHAERELLA. SE HA HALLADO QUE PROMOTORES ESPECIFICOS PARA DICHAS SECUENCIAS SON UTILES PARA LA IDENTIFICACION DE AISLADOS FUNGICOS UTILIZANDO TECNICAS BASADAS EN LA REACCION EN CADENA DE LA POLIMERASA (PCR).
ES95918255T 1994-04-25 1995-04-19 Deteccion de patogenos fungicos usando la reaccion en cadena de la polimerasa. Expired - Lifetime ES2143051T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/233,608 US5585238A (en) 1994-04-25 1994-04-25 Detection of fungal pathogens using the polymerase chain reaction

Publications (1)

Publication Number Publication Date
ES2143051T3 true ES2143051T3 (es) 2000-05-01

Family

ID=22877959

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95918255T Expired - Lifetime ES2143051T3 (es) 1994-04-25 1995-04-19 Deteccion de patogenos fungicos usando la reaccion en cadena de la polimerasa.

Country Status (16)

Country Link
US (2) US5585238A (es)
EP (2) EP0758404B1 (es)
JP (1) JPH10501965A (es)
CN (1) CN1072726C (es)
AT (2) ATE189703T1 (es)
AU (1) AU699766B2 (es)
BR (1) BR9507513A (es)
CA (1) CA2185560A1 (es)
DE (2) DE69515029T2 (es)
DK (1) DK0758404T3 (es)
ES (1) ES2143051T3 (es)
GR (1) GR3032928T3 (es)
PL (3) PL182212B1 (es)
PT (1) PT758404E (es)
RU (1) RU2161196C2 (es)
WO (1) WO1995029260A2 (es)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2193862A1 (es) * 2001-12-20 2003-11-01 Univ Cordoba Metodo para el analisis molecular de la diversidad genetica en razas de fusarium oxysporum f. sp. ciceris mediante rep-pcr.
ES2193861A1 (es) * 2001-12-20 2003-11-01 Univ Cordoba Metodo para la identificacion molecular de fusarium oxysporum f. sp. ciceris y sus razas patogenicas 0,5 y 6 mediante pcr especifica.

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5585238A (en) * 1994-04-25 1996-12-17 Ciba-Geigy Corporation Detection of fungal pathogens using the polymerase chain reaction
US6180339B1 (en) 1995-01-13 2001-01-30 Bayer Corporation Nucleic acid probes for the detection and identification of fungi
DE19615934C1 (de) * 1996-04-22 1997-10-02 Martin Ludwig Dr Niessen Verfahren zur qualitativen und quantitativen analytischen Erfassung des Schimmelpilzes Fusarium Graminearum in Reinkulturen und in jeglichem Probenmaterial
US5792611A (en) * 1996-05-23 1998-08-11 Natural Resources Canada, Canadian Forest Service Detection of plant pathogenic fungi
US6326485B1 (en) * 1996-07-26 2001-12-04 University Of Maryland Biotechnology Institute Assay for perkinsus in shellfish
US5874221A (en) * 1996-08-28 1999-02-23 The United States Of America As Represented By The Secretary Of Agriculture Species specific method for the PCR detection of phythophthora
US5814453A (en) * 1996-10-15 1998-09-29 Novartis Finance Corporation Detection of fungal pathogens using the polymerase chain reaction
US5800997A (en) * 1996-11-01 1998-09-01 Novartis Finance Corporation Detection of maize fungal pathogens using the polymerase chain reaction
EP1021708A1 (en) * 1996-11-11 2000-07-26 Novartis AG Use of biosensors to diagnose plant diseases
WO1998050584A2 (en) * 1997-05-02 1998-11-12 THE GOVERNMENT OF THE UNITED STATES OF AMERICA as represented by THE SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES, c/o Centers for Disease Control and Prevention, Technology Transfer Offic Nucleic acids for detecting aspergillus species and other filamentous fungi
US5827695A (en) * 1997-08-04 1998-10-27 Novartis Finance Corporation Detection of wheat fungal pathogens using the polymerase chain reaction
US6080543A (en) * 1997-12-08 2000-06-27 E. & J. Gallo Winery Detection of fungal pathogens
US6358680B1 (en) 1998-02-20 2002-03-19 Syngenta Participations Ag Detection of wheat and barley fungal pathogens using the polymerase chain reaction
US6248519B1 (en) * 1998-03-11 2001-06-19 E & J Gallo Winery Detection of fermentation-related microorganisms
US6387652B1 (en) * 1998-04-15 2002-05-14 U.S. Environmental Protection Agency Method of identifying and quantifying specific fungi and bacteria
FR2781812B1 (fr) * 1998-07-30 2002-12-20 Lallemand Sa Procede d'identification specifique de levures
DE19840531C2 (de) * 1998-08-28 2003-05-15 Roboscreen Ges Fuer Molekulare Mit Nukleinsäuren beschichtete Reaktionsräume, Verfahren zu ihrer Herstellung und ihre Verwendung
US6221595B1 (en) 1999-03-01 2001-04-24 Syngenta Participations Ag Detection of Monilinia spp. using the polymerase chain reaction
US6469156B1 (en) 1999-04-20 2002-10-22 The United States Of America As Represented By The Department Of Health And Human Services Rapid and sensitive method for detecting histoplasma capsulatum
JP4963765B2 (ja) * 1999-05-28 2012-06-27 イノジェネティックス・ナムローゼ・フェンノートシャップ 臨床的に重要な真菌病原体を検出するための核酸プローブおよび方法
CA2381204A1 (en) * 1999-08-10 2001-02-15 Syngenta Participations Ag Pcr-based detection and quantification of tapesia yallundae and tapesia acuformis
US6319673B1 (en) * 1999-08-10 2001-11-20 Syngenta Participations Ag PCR-based detection and quantification of Tapesia yallundae and Tapesia acuformis
GB2354764A (en) * 1999-09-30 2001-04-04 Mini Agriculture & Fisheries Detection of microorganisms in plant material
US6485907B1 (en) * 2000-01-11 2002-11-26 Syngenta Participations Ag PCR-based detection of Rhizoctonia cerealis
US6287779B1 (en) 2000-01-20 2001-09-11 E. & J. Gallo Winery Detection of fermentation-related microorganisms
CN1436248A (zh) * 2000-06-16 2003-08-13 辛根塔参与股份公司 利用聚合酶链式反应对球腔菌属的检测
US6645720B2 (en) 2001-03-09 2003-11-11 Syngenta Participations Ag Detection of fungal pathogens using the polymerase chain reaction
US6846631B2 (en) * 2001-09-24 2005-01-25 Syngenta Participations Ag Detection of Fusarium species infecting corn using the polymerase chain reaction
DE60234461D1 (de) * 2001-09-26 2009-12-31 Us Gov Health & Human Serv Nukleinsäuren zur identifizierung von pilzen und verfahren zu deren verwendung
US20040166492A1 (en) * 2002-02-05 2004-08-26 Engel Stacia R. Quantitative detection of dekkera and saccharomyces
EP1492886A4 (en) * 2002-04-03 2007-11-21 Syngenta Participations Ag DETECTION OF CERTAIN FUNGICIDE RESISTANT PILZER REGULATORS FOR WHEAT AND BARLEY USING THE POLYMERASE CHAIN REACTION
RU2233888C1 (ru) * 2002-12-15 2004-08-10 Дальневосточный государственный медицинский университет Способ обнаружения микроорганизма "montezuma"
AR048411A1 (es) 2004-03-30 2006-04-26 Monsanto Technology Llc Metodos para controlar patogenos de plantas que utilizan n-fosfonometilglicina
NZ571459A (en) 2006-03-29 2011-09-30 Merial Ltd Vaccine against streptococcus equi
DE102007010311A1 (de) * 2007-02-23 2008-08-28 Thines, Marco, Dr. Organismusspezifisches hybridisierbares Nucleinsäuremolekül
RU2465332C1 (ru) * 2011-08-08 2012-10-27 Общество с ограниченной ответственностью "Лесная диагностика" Набор синтетических олигонуклеотидов для выявления днк возбудителя болезней лиственных пород - гриба polyporus squamosus методом полимеразной цепной реакции
CN104630369B (zh) * 2015-02-13 2016-02-24 湖南农业大学 水稻恶苗病菌的pcr检测方法
CN104846094A (zh) * 2015-05-13 2015-08-19 青岛农业大学 草莓根腐病害多种病原菌分子快速检测方法及应用
PE20211635A1 (es) 2018-07-17 2021-08-24 Bayer Sas Metodos biologicos para controlar hongos fitopatogenicos
JP7399451B2 (ja) * 2019-10-02 2023-12-18 国立大学法人九州大学 バラタナゴ類の判別方法及び判別キット
RU2735911C1 (ru) * 2020-06-01 2020-11-10 Федеральное государственное бюджетное образовательное учреждение высшего образования "Воронежский государственный лесотехнический университет имени Г.Ф. Морозова" Способ идентификации гриба Heterobasidion annosum
CN113637788B (zh) * 2021-07-30 2024-09-20 贵州茅台酒股份有限公司 一种构建重组质粒对样本中真菌进行绝对定量的方法
CN115838639B (zh) * 2022-12-17 2024-02-13 昆明理工大学 白茅种子内生真菌df101及其应用
CN116790779A (zh) * 2023-08-14 2023-09-22 广东美格基因科技有限公司 一种微生物种群绝对丰度定量的内参组合物、试剂盒及方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5447848A (en) * 1986-01-22 1995-09-05 Amoco Corporation Detection of campylobacter with nucleic acid probes
CA2025181A1 (en) * 1989-10-12 1991-04-13 William G. Weisburg Nucleic acid probes and methods for detecting fungi
US5126239A (en) * 1990-03-14 1992-06-30 E. I. Du Pont De Nemours And Company Process for detecting polymorphisms on the basis of nucleotide differences
US5585238A (en) * 1994-04-25 1996-12-17 Ciba-Geigy Corporation Detection of fungal pathogens using the polymerase chain reaction

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2193862A1 (es) * 2001-12-20 2003-11-01 Univ Cordoba Metodo para el analisis molecular de la diversidad genetica en razas de fusarium oxysporum f. sp. ciceris mediante rep-pcr.
ES2193861A1 (es) * 2001-12-20 2003-11-01 Univ Cordoba Metodo para la identificacion molecular de fusarium oxysporum f. sp. ciceris y sus razas patogenicas 0,5 y 6 mediante pcr especifica.
ES2193862B1 (es) * 2001-12-20 2005-03-01 Universidad De Cordoba Metodo para el analisis molecular de la diversidad genetica en razas de fusarium oxysporum f. sp. ciceris mediante rep-pcr.
ES2193861B1 (es) * 2001-12-20 2005-03-01 Universidad De Cordoba Metodo para la identificacion molecular de fusarium oxysporum f. sp. ciceris y sus razas patogenicas 0,5 y 6 mediante pcr especifica.

Also Published As

Publication number Publication date
JPH10501965A (ja) 1998-02-24
DE69515029T2 (de) 2000-09-21
WO1995029260A3 (en) 1995-12-28
DE69534933T2 (de) 2006-09-07
EP0955381A3 (en) 2003-06-11
PL182212B1 (pl) 2001-11-30
BR9507513A (pt) 1997-09-02
EP0758404B1 (en) 2000-02-09
RU2161196C2 (ru) 2000-12-27
DE69534933D1 (de) 2006-05-24
CN1147276A (zh) 1997-04-09
AU699766B2 (en) 1998-12-17
EP0955381A2 (en) 1999-11-10
PT758404E (pt) 2000-06-30
EP0758404A1 (en) 1997-02-19
US5585238A (en) 1996-12-17
GR3032928T3 (en) 2000-07-31
DE69515029D1 (de) 2000-03-16
WO1995029260A2 (en) 1995-11-02
PL316962A1 (en) 1997-02-17
AU2424495A (en) 1995-11-16
EP0955381B1 (en) 2006-04-12
CA2185560A1 (en) 1995-11-02
ATE189703T1 (de) 2000-02-15
CN1072726C (zh) 2001-10-10
US5955274A (en) 1999-09-21
DK0758404T3 (da) 2000-07-24
PL182188B1 (pl) 2001-11-30
PL182002B1 (pl) 2001-10-31
ATE323177T1 (de) 2006-04-15

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