DE69622347D1 - Oligonukleotid-Primer für Amplifizierung von HCV-Nukleinsäure - Google Patents
Oligonukleotid-Primer für Amplifizierung von HCV-NukleinsäureInfo
- Publication number
- DE69622347D1 DE69622347D1 DE69622347T DE69622347T DE69622347D1 DE 69622347 D1 DE69622347 D1 DE 69622347D1 DE 69622347 T DE69622347 T DE 69622347T DE 69622347 T DE69622347 T DE 69622347T DE 69622347 D1 DE69622347 D1 DE 69622347D1
- Authority
- DE
- Germany
- Prior art keywords
- nucleic acid
- primers
- amplification
- hcv
- oligonucleotide primer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108020004707 nucleic acids Proteins 0.000 title abstract 4
- 102000039446 nucleic acids Human genes 0.000 title abstract 4
- 230000003321 amplification Effects 0.000 title abstract 3
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 3
- -1 HCV nucleic acid Chemical class 0.000 title 1
- 108091034117 Oligonucleotide Proteins 0.000 title 1
- 241000711549 Hepacivirus C Species 0.000 abstract 7
- 150000007523 nucleic acids Chemical group 0.000 abstract 4
- 238000000034 method Methods 0.000 abstract 3
- 238000003752 polymerase chain reaction Methods 0.000 abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 230000035945 sensitivity Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/706—Specific hybridization probes for hepatitis
- C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Communicable Diseases (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US773995P | 1995-11-29 | 1995-11-29 |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69622347D1 true DE69622347D1 (de) | 2002-08-22 |
DE69622347T2 DE69622347T2 (de) | 2003-02-13 |
Family
ID=21727873
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69622347T Expired - Lifetime DE69622347T2 (de) | 1995-11-29 | 1996-11-21 | Oligonukleotid-Primer für Amplifizierung von HCV-Nukleinsäure |
Country Status (8)
Country | Link |
---|---|
US (1) | US5837442A (de) |
EP (1) | EP0776981B1 (de) |
JP (2) | JP3814352B2 (de) |
AT (1) | ATE220726T1 (de) |
CA (1) | CA2191182C (de) |
DE (1) | DE69622347T2 (de) |
DK (1) | DK0776981T3 (de) |
ES (1) | ES2179909T3 (de) |
Families Citing this family (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7070925B1 (en) * | 1996-07-16 | 2006-07-04 | Gen-Probe Incorporated | Method for determining the presence of an RNA analyte in a sample using a modified oligonucleotide probe |
DK0866071T3 (da) * | 1997-03-20 | 2005-01-17 | Hoffmann La Roche | Modificerede primere |
US6638714B1 (en) * | 1999-02-03 | 2003-10-28 | Ortho-Clinical Diagnostics, Inc. | Oligonucleotide primers for efficient detection of hepatitis C virus (HCV) and methods of use thereof |
ES2230027T3 (es) * | 1999-02-03 | 2005-05-01 | Ortho-Clinical Diagnostics, Inc. | Cebadores iligonucleotidos para la transcripcion inversa eficaz de arn del virus de la hepatitis c(vhc) y procedimientos para su uso. |
CA2304260C (en) * | 1999-04-20 | 2009-03-24 | Japan Bioindustry Association | Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method and method for analyzing data obtained by the method |
US6586584B2 (en) | 2001-01-29 | 2003-07-01 | Becton, Dickinson And Company | Sequences and methods for detection of Hepatitis C virus |
CN100523216C (zh) * | 2001-03-02 | 2009-08-05 | 匹兹堡大学 | Pcr方法 |
EP1236804A1 (de) * | 2001-03-02 | 2002-09-04 | Boehringer Mannheim Gmbh | Verfahren zur Bestimmung von Nukleinsäuren mittels einer Kontrolle |
US20030152942A1 (en) * | 2001-05-09 | 2003-08-14 | Lance Fors | Nucleic acid detection in pooled samples |
KR100440852B1 (ko) * | 2001-05-10 | 2004-07-19 | 주식회사 바이로메드 | 레트로바이러스에 특이적인 올리고뉴클레오티드 프라이머및 이를 이용한 레트로바이러스 농도 측정방법 |
US6946245B2 (en) * | 2001-12-04 | 2005-09-20 | Quest Diagnostics Investments Incorporated | Oligonucleotides and methods for detecting hepatitis C viral nucleic acids |
WO2004011612A2 (en) * | 2002-07-26 | 2004-02-05 | Abbott Laboratories | Method of detecting and quantifying hepatitis c virus |
US7820030B2 (en) | 2003-04-16 | 2010-10-26 | Handylab, Inc. | System and method for electrochemical detection of biological compounds |
WO2005055804A2 (en) * | 2003-12-02 | 2005-06-23 | Musc Foundation For Research Development | Methods and compositions for diagnosing epithelial cell cancer |
WO2005084254A2 (en) * | 2004-02-27 | 2005-09-15 | Musc Foundation For Research Development | Enhanced detection of rna using a panel of truncated gene-specific primers for reverse transcription |
AU2005271960B2 (en) | 2004-07-09 | 2011-12-08 | University Of Pittsburgh-Of The Commonwealth System Of Higher Education | Identification of markers in lung and breast cancer |
EP1799858A4 (de) * | 2004-09-20 | 2009-03-04 | Univ Pittsburgh | Multimodus-multiplexlöschverfahren |
WO2006085328A2 (en) * | 2005-02-14 | 2006-08-17 | Mor Research Applications Ltd. | Method and kit for diagnosing and predicting hepatitis c virus-related neoplastic transformation |
US7465561B2 (en) * | 2005-06-30 | 2008-12-16 | Roche Molecular Systems, Inc. | Probes and methods for hepatitis C virus typing using single probe analysis |
EP1910397A4 (de) * | 2005-07-15 | 2009-07-01 | Applera Corp | Entwurf einer heissstart-rückübertragung durch primer |
CA2571904A1 (en) * | 2006-02-15 | 2007-08-15 | Fio Corporation | System and method of detecting pathogens |
US8119352B2 (en) * | 2006-06-20 | 2012-02-21 | Cepheld | Multi-stage amplification reactions by control of sequence replication times |
CA2580589C (en) | 2006-12-19 | 2016-08-09 | Fio Corporation | Microfluidic detection system |
CA2682826C (en) | 2007-04-02 | 2013-08-13 | Fio Corporation | System and method of deconvolving multiplexed fluorescence spectral signals generated by quantum dot optical coding technology |
WO2009000084A1 (en) | 2007-06-22 | 2008-12-31 | Fio Corporation | Systems and methods for manufacturing quantum dot-doped polymer microbeads |
JP5507454B2 (ja) | 2007-07-09 | 2014-05-28 | フィオ コーポレイション | 試験サンプル中の標的分子の蛍光検出の向上のためのシステムおよび方法 |
JP5721161B2 (ja) * | 2007-08-31 | 2015-05-20 | 国立大学法人鳥取大学 | TERT遺伝子のcDNAの製造、それを用いた核酸増幅法、検出方法、それらに用いるプライマー、及び、それらを用いた腫瘍の診断キット |
US8551763B2 (en) | 2007-10-12 | 2013-10-08 | Fio Corporation | Flow focusing method and system for forming concentrated volumes of microbeads, and microbeads formed further thereto |
US9792809B2 (en) | 2008-06-25 | 2017-10-17 | Fio Corporation | Bio-threat alert system |
CA2735273A1 (en) | 2008-08-29 | 2010-03-04 | Fio Corporation | A single-use handheld diagnostic test device, and an associated system and method for testing biological and environmental test samples |
CN104374932A (zh) | 2009-01-13 | 2015-02-25 | Fio公司 | 与电子设备和快速诊断测试中的测试盒结合使用的手持诊断测试设备 |
Family Cites Families (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
US5322770A (en) | 1989-12-22 | 1994-06-21 | Hoffman-Laroche Inc. | Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription |
US5310652A (en) | 1986-08-22 | 1994-05-10 | Hoffman-La Roche Inc. | Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription |
CN1049686C (zh) | 1987-11-18 | 2000-02-23 | 希龙股份有限公司 | 非a和非b肝炎病毒的诊断及疫苗 |
HUT54896A (en) | 1989-03-17 | 1991-04-29 | Chiron Corp | Process for producing aquous diagnosticum and vaccine of nanbv |
GEP20002164B (en) | 1989-05-18 | 2000-07-10 | Novartis Vaccines & Diagnostic | Oligomers, method for detecting of hcv sequence, kit for its detecting, method for production of the blood free from hcv |
US5035996A (en) | 1989-06-01 | 1991-07-30 | Life Technologies, Inc. | Process for controlling contamination of nucleic acid amplification reactions |
DE69131891T2 (de) | 1990-02-16 | 2000-06-15 | F. Hoffmann-La Roche Ag, Basel | Verbesserungen in der spezifität und zweckmässigkeit der polymerase-kettenreaktion |
CA2087724C (en) | 1990-07-24 | 2003-09-16 | John J. Sninsky | Reduction of non-specific amplification during in vitro nucleic acid amplification using modified nucleic acid bases |
US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
EP0487218B1 (de) | 1990-10-31 | 1997-12-29 | Tosoh Corporation | Verfahren zum Nachweis oder Quantifizierung von Zielnukleinsäuren |
US5994056A (en) | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
EP0529493B1 (de) * | 1991-08-27 | 1997-12-17 | F. Hoffmann-La Roche Ag | Verfahren und Reagenzien zum Nachweis von Hepatitis C |
EP1262560A2 (de) * | 1992-05-29 | 2002-12-04 | Abbott Laboratories | Methode zur Herstellung von cDNA ausgehend von einer RNA Zielsequenz in einer Probe |
DK0632134T3 (da) * | 1993-07-01 | 2001-12-03 | Hoffmann La Roche | Reagenser og fremgangsmåder til koblet revers transkription ved høj temperatur og polymerase-kædereaktioner |
AU7718594A (en) * | 1993-09-03 | 1995-03-22 | United States Of America As Represented By The Secretary Department Of Health And Human Services | Method and compositions for primer specific solid-phase detection of pcr products |
US5491063A (en) | 1994-09-01 | 1996-02-13 | Hoffmann-La Roche Inc. | Methods for in-solution quenching of fluorescently labeled oligonucleotide probes |
US5571673A (en) | 1994-11-23 | 1996-11-05 | Hoffmann-La Roche Inc. | Methods for in-solution quenching of fluorescently labeled oligonucleotide probes |
US9105210B2 (en) | 2012-06-29 | 2015-08-11 | Microsoft Technology Licensing, Llc | Multi-node poster location |
-
1996
- 1996-10-28 US US08/738,928 patent/US5837442A/en not_active Expired - Lifetime
- 1996-11-21 ES ES96118704T patent/ES2179909T3/es not_active Expired - Lifetime
- 1996-11-21 AT AT96118704T patent/ATE220726T1/de active
- 1996-11-21 EP EP96118704A patent/EP0776981B1/de not_active Expired - Lifetime
- 1996-11-21 DE DE69622347T patent/DE69622347T2/de not_active Expired - Lifetime
- 1996-11-21 DK DK96118704T patent/DK0776981T3/da active
- 1996-11-25 CA CA002191182A patent/CA2191182C/en not_active Expired - Lifetime
- 1996-11-28 JP JP31802796A patent/JP3814352B2/ja not_active Expired - Lifetime
-
2005
- 2005-08-15 JP JP2005235224A patent/JP3903059B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
JP3814352B2 (ja) | 2006-08-30 |
US5837442A (en) | 1998-11-17 |
CA2191182A1 (en) | 1997-05-30 |
DK0776981T3 (da) | 2002-11-11 |
CA2191182C (en) | 2004-09-21 |
DE69622347T2 (de) | 2003-02-13 |
EP0776981B1 (de) | 2002-07-17 |
ES2179909T3 (es) | 2003-02-01 |
JP2006020642A (ja) | 2006-01-26 |
JP3903059B2 (ja) | 2007-04-11 |
EP0776981A3 (de) | 1998-08-19 |
JPH09163991A (ja) | 1997-06-24 |
EP0776981A2 (de) | 1997-06-04 |
ATE220726T1 (de) | 2002-08-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |