EP2316958A3 - Procédés et compositions pour obtenir des plantes transgéniques sans marqueurs - Google Patents

Procédés et compositions pour obtenir des plantes transgéniques sans marqueurs Download PDF

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Publication number: EP2316958A3
Authority: EP; European Patent Office
Prior art keywords: compositions; methods; transgenic plants; interest; free transgenic
Prior art date: 2006-05-12
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Granted

Application number

EP11155239A

Other languages

German (de)

English (en)

Other versions

EP2316958B1 (fr

EP2316958A2 (fr

Inventor

Xudong XE

Michael W. Petersen

Shinhshieh Huang

Paul S. Chomet

David Walters

Susan Johnson

Larry Gilbertson

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Monsanto Technology LLC

Original Assignee

Monsanto Technology LLC

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2006-05-12

Filing date

2007-05-11

Publication date

2011-05-18

Family has litigation

First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=38663159&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EP2316958(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.

2007-05-11 Application filed by Monsanto Technology LLC filed Critical Monsanto Technology LLC

2011-05-04 Publication of EP2316958A2 publication Critical patent/EP2316958A2/fr

2011-05-18 Publication of EP2316958A3 publication Critical patent/EP2316958A3/fr

2015-08-19 Application granted granted Critical

2015-08-19 Publication of EP2316958B1 publication Critical patent/EP2316958B1/fr

Status Not-in-force legal-status Critical Current

2027-05-11 Anticipated expiration legal-status Critical

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
- C12N15/8265—Transgene containment, e.g. gene dispersal
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8205—Agrobacterium mediated transformation
- C—CHEMISTRY; METALLURGY
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
- C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
- C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
- C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
- C—CHEMISTRY; METALLURGY
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- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
- C—CHEMISTRY; METALLURGY
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- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/823—Reproductive tissue-specific promoters
- C12N15/8231—Male-specific, e.g. anther, tapetum, pollen
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/823—Reproductive tissue-specific promoters
- C12N15/8234—Seed-specific, e.g. embryo, endosperm
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
- C12N15/8263—Ablation; Apoptosis
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- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
- C12N15/8267—Seed dormancy, germination or sprouting
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- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
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- C12N15/09—Recombinant DNA-technology
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- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
- C12N15/8289—Male sterility
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- C12N15/09—Recombinant DNA-technology
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- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
- C12N15/829—Female sterility
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- C12N15/09—Recombinant DNA-technology
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- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8291—Hormone-influenced development
- C12N15/8294—Auxins
- C—CHEMISTRY; METALLURGY
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)

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Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Molecular Biology (AREA)
General Health & Medical Sciences (AREA)
Microbiology (AREA)
Biochemistry (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
Plant Pathology (AREA)
Cell Biology (AREA)
Reproductive Health (AREA)
Pregnancy & Childbirth (AREA)
Developmental Biology & Embryology (AREA)
Virology (AREA)
Endocrinology (AREA)
Physiology (AREA)
Medicinal Chemistry (AREA)
Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Analytical Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Botany (AREA)
Nutrition Science (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Mycology (AREA)
Immunology (AREA)
Environmental Sciences (AREA)
Oil, Petroleum & Natural Gas (AREA)
Pest Control & Pesticides (AREA)
Insects & Arthropods (AREA)

EP11155239.4A 2006-05-12 2007-05-11 Procédés et compositions pour obtenir des plantes transgéniques sans marqueurs Not-in-force EP2316958B1 (fr)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US79987506P	2006-05-12	2006-05-12
EP07811889.0A EP2016181B1 (fr)	2006-05-12	2007-05-11	Procédés et compositions pour l'élaboration de plantes transgéniques sans marqueur

Related Parent Applications (2)

Application Number	Title	Priority Date	Filing Date
EP07811889.0 Division		2007-05-11
EP07811889.0A Division EP2016181B1 (fr)	2006-05-12	2007-05-11	Procédés et compositions pour l'élaboration de plantes transgéniques sans marqueur

Publications (3)

Publication Number	Publication Date
EP2316958A2 EP2316958A2 (fr)	2011-05-04
EP2316958A3 true EP2316958A3 (fr)	2011-05-18
EP2316958B1 EP2316958B1 (fr)	2015-08-19

Family

ID=38663159

Family Applications (4)

Application Number	Title	Priority Date	Filing Date
EP14182312.0A Withdrawn EP2811025A3 (fr)	2006-05-12	2007-05-11	Procédés et compositions pour obtenir des plantes transgéniques sans marqueurs
EP07811889.0A Not-in-force EP2016181B1 (fr)	2006-05-12	2007-05-11	Procédés et compositions pour l'élaboration de plantes transgéniques sans marqueur
EP11155202.2A Not-in-force EP2316957B1 (fr)	2006-05-12	2007-05-11	Procédés et compositions pour obtenir des plantes transgéniques sans marqueurs
EP11155239.4A Not-in-force EP2316958B1 (fr)	2006-05-12	2007-05-11	Procédés et compositions pour obtenir des plantes transgéniques sans marqueurs

Family Applications Before (3)

Application Number	Title	Priority Date	Filing Date
EP14182312.0A Withdrawn EP2811025A3 (fr)	2006-05-12	2007-05-11	Procédés et compositions pour obtenir des plantes transgéniques sans marqueurs
EP07811889.0A Not-in-force EP2016181B1 (fr)	2006-05-12	2007-05-11	Procédés et compositions pour l'élaboration de plantes transgéniques sans marqueur
EP11155202.2A Not-in-force EP2316957B1 (fr)	2006-05-12	2007-05-11	Procédés et compositions pour obtenir des plantes transgéniques sans marqueurs

Country Status (10)

Country	Link
US (7)	US8076536B2 (fr)
EP (4)	EP2811025A3 (fr)
JP (7)	JP5330231B2 (fr)
CN (1)	CN101490264B (fr)
AU (1)	AU2007249224B2 (fr)
BR (2)	BR122016030514B1 (fr)
CA (3)	CA2653231C (fr)
MX (1)	MX295027B (fr)
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BRPI0711634A2 (pt)	2012-01-17
US20210163977A1 (en)	2021-06-03
EP2316957B1 (fr)	2014-09-10
JP5330231B2 (ja)	2013-10-30
US20170253937A1 (en)	2017-09-07
US8237016B2 (en)	2012-08-07
EP2316958B1 (fr)	2015-08-19
EP2016181B1 (fr)	2013-04-17
CN101490264A (zh)	2009-07-22
AU2007249224B2 (en)	2013-03-21
CA2653231C (fr)	2016-04-12
US8076536B2 (en)	2011-12-13
WO2007134234A8 (fr)	2008-06-12
EP2811025A3 (fr)	2015-04-15
BR122016030514B1 (pt)	2019-04-16
JP2013223513A (ja)	2013-10-31
EP2811025A2 (fr)	2014-12-10
US20130198895A1 (en)	2013-08-01
BRPI0711634B1 (pt)	2018-06-19
EP2316958A2 (fr)	2011-05-04
JP2013223512A (ja)	2013-10-31
CN101490264B (zh)	2013-05-22
US8829275B2 (en)	2014-09-09
US20070266456A1 (en)	2007-11-15
CA2917552A1 (fr)	2007-11-22
JP2020171330A (ja)	2020-10-22
EP2316957A3 (fr)	2011-08-31
US11629357B2 (en)	2023-04-18
US20190345507A1 (en)	2019-11-14
US20150052642A1 (en)	2015-02-19
JP6647844B2 (ja)	2020-02-14
US20120131699A1 (en)	2012-05-24
JP2019013259A (ja)	2019-01-31
AU2007249224A1 (en)	2007-11-22
WO2007134234A3 (fr)	2008-04-03
MX295027B (es)	2012-01-20
US10240165B2 (en)	2019-03-26
JP2016032479A (ja)	2016-03-10
ZA200809504B (en)	2009-12-30
US9540700B2 (en)	2017-01-10
MX2008014506A (es)	2008-11-27
CA2653231A1 (fr)	2007-11-22
JP2009536835A (ja)	2009-10-22
EP2316957A2 (fr)	2011-05-04
EP2016181A2 (fr)	2009-01-21
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CA2917552C (fr)	2018-09-25
CA3014139A1 (fr)	2007-11-22

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