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ZA200809504B - Methods and compositions for obtaining marker-free transgenic plants - Google Patents

Methods and compositions for obtaining marker-free transgenic plants

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Publication number
ZA200809504B
ZA200809504B ZA200809504A ZA200809504A ZA200809504B ZA 200809504 B ZA200809504 B ZA 200809504B ZA 200809504 A ZA200809504 A ZA 200809504A ZA 200809504 A ZA200809504 A ZA 200809504A ZA 200809504 B ZA200809504 B ZA 200809504B
Authority
ZA
South Africa
Prior art keywords
compositions
methods
transgenic plants
free transgenic
obtaining marker
Prior art date
Application number
ZA200809504A
Other languages
English (en)
Inventor
Xudong Ye
Michael W Petersen
Shihshieh Huang
Paul S Chomet
David Walters
Susan Johnson
Larry Gilbertson
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=38663159&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ZA200809504(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of ZA200809504B publication Critical patent/ZA200809504B/xx

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8265—Transgene containment, e.g. gene dispersal
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    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
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    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
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    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
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    • C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
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    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
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    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
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    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
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    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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ZA200809504A 2006-05-12 2008-11-06 Methods and compositions for obtaining marker-free transgenic plants ZA200809504B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US79987506P 2006-05-12 2006-05-12

Publications (1)

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ZA200809504B true ZA200809504B (en) 2009-12-30

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Country Link
US (7) US8076536B2 (xx)
EP (4) EP2016181B1 (xx)
JP (7) JP5330231B2 (xx)
CN (1) CN101490264B (xx)
AU (1) AU2007249224B2 (xx)
BR (2) BRPI0711634B1 (xx)
CA (3) CA3014139A1 (xx)
MX (1) MX295027B (xx)
WO (1) WO2007134234A2 (xx)
ZA (1) ZA200809504B (xx)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2016181B1 (en) 2006-05-12 2013-04-17 Monsanto Technology, LLC Methods and compositions for obtaining marker-free transgenic plants
WO2010009353A1 (en) * 2008-07-16 2010-01-21 Monsanto Technology Llc Methods and vectors for producing transgenic plants
AU2009284260A1 (en) * 2008-08-20 2010-02-25 Basf Plant Science Gmbh Transgenic plants comprising as transgene a phosphatidate cytidylyltransferase
KR101099614B1 (ko) 2009-05-21 2011-12-28 대한민국 식물형질전환용 운반체, 식물형질전환체, 식물형질전환 방법 및 이를 이용한 유전자형의 판별방법
CN102102108B (zh) * 2009-12-01 2015-04-29 北京未名凯拓作物设计中心有限公司 一种利用双t-dna+1载体培育高效无选择标记转基因作物的方法
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