CN1067555C - Application of medicinal combination containing termite population and main nest bacterial nursery - Google Patents

Abstract

The invention provides an application of a pharmaceutical composition comprising a dried body of a termite-to-nest fungus containing termite populations. In the medicine composition, the weight ratio of the termites to the termite main nest fungus is 1:15-20. The pharmaceutical composition can be used for treating mammals including human chronic hepatitis, anti-inflammation and analgesia, treating arthritis and infertility, has antitussive effect, and can also improve its cold resistance. The invention turns harm into wealth and makes use of termites that harm forests.

Description

Use of a pharmaceutical composition comprising a termite population and its host colony

The present invention relates to the application of a pharmaceutical composition, in particular to the application of a pharmaceutical composition comprising termite populations and their main nest fungus.

As we all know, termites are one of the five major pests recognized in the world, harming trees, embankments, earth dams, and even houses. There are more than 2,000 known species of termites in the world, and there are more than 360 species in my country, which are distributed in 25 provinces (districts) and municipalities directly under the Central Government in the south of Dandong, Liaoning. Odontotermes formosanus (Shiraki), which is widely distributed in the southern provinces of my country, harms more than 70 species of forest trees such as Chinese fir, and is a major pest in agriculture and water conservancy. According to investigations, there can be as many as 3,000 black-winged soil termite nests in a thousand-acre fir forest, and the population is extremely rich. People have known for a long time that the termite itself can be edible, and the chicken mushroom grown on the fungus garden in the termite nest is a delicious dish especially, and some domestic and foreign people eat it.

Termites belong to the Insecta Isoptera. Like other insects, especially ants and bees, which belong to the world's three major social insects, termites contain 18 kinds of amino acids and various trace elements including selenium and zinc needed by the human body. It is a miniature treasure trove of nutrition, which is used for health care and treatment, with great benefits and no small disadvantages. At the same time, termites live underground in a camp, living in a humid ecological environment and have great living and reproductive capabilities; even if the termites die, they will not grow mold and rot in the nest; termites eat the basic nutrients (sap) of various trees; The gallinaceous fungus symbiotic with the termites has both edible value and medicinal effect (contains total flavonoids, which has the effect of calming the nerves).

The medicinal value of termites and their fungi have been reported in traditional Chinese medicine.

Li Shizhen has recorded termites in "Compendium of Materia Medica". Regarding ant eggs, Li Shizhen said, "The name of the eggs is 蚳. Mountain people dig them up, and some go to fight with stones. Stone people eat them, so the beans that are fed in "Nei Ze" and "Zhou Guan" are also fermented." In the Qing Dynasty, Zhao Xuemin said in "Compendium of Materia Medica Supplements" that ants and ant eggs are "sweet in nature and flavor, slightly poisonous, beneficial to energy, luster (beauty), and lactating". For lack of physical strength after illness, postpartum lack of milk, etc., 9-15g stir-fried yellow, grind the noodles and take it after mixing.

The high-quality fungus garden of termites, Li Shizhen also recorded in "Compendium of Materia Medica" that "termite mud (i.e. ant nest) is mainly used to treat malignant sores and swollen toxins. Those who use pine wood are fried black with yellow dan, ground and fragrant. To heal is to stop."

The gallinaceous fungus symbiotic with the termite fungus can be used for medicine. Li Shizhen pointed out in "Compendium of Materia Medica" that the gallinaceous fungus "smells sweet, flat, and non-toxic. It is mainly used to benefit the stomach, clear the mind, and treat hemorrhoids." "Chinese Medicine Sea" also pointed out that its nature and flavor belong to "sweet, flat, non-toxic. It enters the heart, lung, and spleen meridians, and it is mainly used to strengthen the spleen and stomach, replenish qi and clear the mind.

It can be seen from the above that termite nests have been widely used in the medical field since ancient times, and have miraculous effects such as nourishing energy, tonifying deficiency and invigorating the spleen.

In recent years, some applications to termites have been reported. CN1090760A proposes a health-care medicine made by using pure termites as raw materials, extracts obtained by soaking and extracting, and mixing with hawthorn powder. The medicine has the effect of improving the immune function of the human body and has a therapeutic effect on certain tumors .

In "Modern Applied Medicine", Vol. 10, No. 5 (1993), pages 29-30, the research on the anti-tumor activity and immunomodulatory effect of termite alcohol extract on S180 tumor-bearing mice was reported.

CN1098628A provides termites and their bacterial gardens as raw materials, water extraction and alcohol extraction to obtain extracts, the extracts are made into oral liquids or granulated after concentration and drying, and the obtained products have the functions of nourishing and refreshing, strengthening the spleen and protecting the liver, and prolonging life. Efficacy, and pointed out that it can improve the body's immunity, enhance memory and alertness, etc.

Although the medical value of termite and its fungus has been described and utilized in the prior art, the effect of termite and its fungus on treating diseases is still far from clear.

In addition, because termites are harmful to trees, people have been devoting themselves to killing termites for a long time, but have not effectively utilized termites and ant nest resources while luring and killing termites and destroying termite nests.

The purpose of the present invention is to effectively utilize termites and their fungus gardens, turn harm into wealth, and provide medical and health care applications of pharmaceutical compositions containing termites and their fungus gardens.

In order to achieve the above object, the pharmaceutical composition provided by the present invention contains the dried body of the termite main nest bacteria nursery containing termite populations in the preparation of treatment of mammals including human chronic viral hepatitis, avoiding liver damage, anti-inflammation, detumescence, treatment of joints Inflammation, and the application in the medicine of antitussive, and the application in the health-care medicine that improves mammalian body including the human body cold tolerance, the present invention further provides the pharmaceutical composition that comprises the dried body of the termite main nest bacterial nursery containing termite population Application in preparation of medicine for treating infertility in mammals including humans.

In the pharmaceutical composition containing the dried body of the termite main nest fungus containing termite populations, the weight ratio of the termites in the medicine to the dried termite main nest fungus is 1:15-20.

The termite is preferably Odontotermes formosanus (Shiraki), and the termite main nest fungus is Termitomyces albuminosus (Berk) Heim).

The pharmaceutical composition may or may not contain other medicinal or non-medicinal ingredients, such as other traditional Chinese medicine ingredients.

The above-mentioned pharmaceutical composition comprising the dried body of the termite main nest fungus containing termite populations is prepared in steps:

1) cut off the ant tunnel that termites escape, make the fungus contain termites, preferably make most of the termite colonies in the fungus;

2) dig out the fungus garden;

3) Dehydrating and drying the above-mentioned excavated termite main nest bacteria nursery containing termite populations, such as drying at about 80° C., and then crushing and removing impurities;

4) Sterilization.

Because the components of termites and their fungus gardens are relatively complex, and there is not much harm to them being eaten, the present invention adopts the method of directly drying termites and their fungus gardens, avoiding the loss of active ingredients, and making the termites and their fungus gardens dry. method is easier to implement.

The present invention further includes the pharmaceutical composition prepared by the above method and preparations containing the pharmaceutical composition, such as tablets, capsules and the like.

Through the present invention, termite resources are effectively utilized, especially black-winged soil termites and their fungus nests that harm my country's masson pine, Chinese fir forests, rubber forests or eucalyptus forests, turning harm into treasure.

At the same time, the invention provides new medicinal raw materials. It can prevent people from excessively utilizing ant resources beneficial to plant growth, such as the use of Daheishan ants as medicinal raw materials, thereby achieving the effect of protecting beneficial insects.

The present invention will be described in detail below.

The present invention selects the termite main nest bacterial garden that contains termite population, particularly the termite main nest bacterial garden that contains black winged soil termite, and it is widely distributed in most areas of my country, such as Liaoning, Guangdong, Guangxi, Hunan, Hubei, Shandong, Shanxi , Jiangsu, Zhejiang, Jiangxi, Taiwan, Fujian, Anhui, Sichuan, Yunnan, Shaanxi, Tibet, Hebei, Henan and other places, it is also distributed abroad, such as Myanmar, Thailand, Vietnam, etc. In order to ensure the quality of the prepared medicine is mainly based on winter and spring (October-April of the next year), at first the live nest of the black-winged soil termite is selected, and the depth of its main nest is about 1.5-3.0m. 0.5-1 meter. Cut off the ant tunnel where termites escape, so that most of the termite colonies are in the fungus garden. After digging out, bring the fungus garden back to the room as soon as possible; choose a constant temperature oven with a yellow-brown main nest (containing termite populations), preferably Dehydrate and dry the fungus at about 80°C; then, use a pulverizer to crush the dried termite fungus, and sieve it, such as passing through a 80-mesh sieve, to collect the dry powder; the dry powder is sterilized, such as at a constant temperature of 100°C Bacteria for 60 minutes, through the method known to those skilled in the art, the pharmaceutical composition of the present invention comprising the dry body of the termite main nest fungus containing termite populations is prepared.

The medicinal activity of the pharmaceutical composition of the present invention comprising a termite population and its host colony is illustrated as follows.

Studies have shown that termites and their nests contain 18 kinds of amino acids needed by the human body, various trace elements including selenium and zinc, and vitamin E. Among them, termites contain higher protein, formic acid, formic aldehyde and cholesterol similar to adrenal glands. Corticosteroid-like substances. Chicken bacterium contains a large amount of total flavonoids (flavonoids have a calming effect). A large number of animal experiments have proved that termites and their colonies have obvious anti-inflammatory, swelling, and analgesic effects, can reduce alanine aminotransferase, protect the liver from poisonous damage, and promote the repair function of liver cells after acute injury. Clinical use in human experiments also shows that taking the pharmaceutical composition of the present invention has curative effects on rheumatoid and rheumatoid arthritis, senile osteoporosis, insomnia, constipation and other diseases, and has the effects of invigorating the stomach, anti-aging and enhancing immunity And so on.

Chinese medicine attaches great importance to the "righteousness" of the human body. Insufficient righteousness in the human body will not be able to effectively resist the occurrence of diseases or eliminate the invasion of pathogenic factors; but sufficient righteousness will overcome the harm of pathogenic factors during the development of the disease and facilitate recovery. It can be seen that whether the righteousness is strong or not is closely related to the occurrence, development and recovery of the disease. Therefore, those who are weak in constitution and lack of righteousness need to use tonification to enhance their constitution and disease resistance.

Different etiologies act on different constitutions to form deficiency syndromes. Although the nature of the pathogenesis and the tendency of transmission and change are extremely complex and diverse, the changes in the pathogenesis are nothing more than weakness of qi, blood, yin and yang, and deficiency of heart, lung, spleen, stomach, liver and kidney. Among them, kidney and spleen are extremely important in the pathogenesis of deficiency. The spleen and stomach are the source of essence and qi biochemistry, if the spleen and stomach are weak, the water and food will not be transformed, the whole body will lose nourishment, and all will be exhausted. The famous Chinese medicine book "Medical Law" said: "If the diet is not good, the blood will not grow, and if the blood does not grow, the yin will not be enough to match the yang, and all the internal organs will be damaged."

For the treatment of deficiency syndrome, it should be based on the principles of "replenish the deficiency", "replenish the loss", "warm the laborer" and "warm the Qi for the lack of shape; law. However, the qi and blood of the human viscera come from the innate, and the nourishment comes from the day after tomorrow. Therefore, regulating the spleen and kidney is the key to treating deficiency syndrome.

The pharmaceutical composition comprising the termite population and its main nest fungus of the present invention is very suitable for the treatment and health care of debilitating diseases such as deficiency and insomnia. Practical experience also shows that this product is safe and effective in strengthening the body and tonifying deficiency.

Hepatitis is caused by hepatitis virus infection. Traditional Chinese medicine believes that damp-heat virus invades the human body, and evil will damage the righteousness, leading to deficiency of both yin and yang in the human body. Damp-heat accumulates in the liver, leading to stagnation of liver and gallbladder qi. The liver belongs to wood, and the kidney belongs to water. Among the five elements, the liver is The son of the kidney, the child's disease traps the mother's qi, which damages the kidney. The liver and kidney are of the same origin, and the kidney is the innate foundation, which manifests as upset and irritability, dry mouth, abdominal distension, dull pain in the liver area, etc. Qi stagnation for a long time will lead to Qi deficiency, manifested as Qi deficiency, fatigue, and mental fatigue. The pharmaceutical composition of the invention has the obvious functions of strengthening body constitution, strengthening health and eliminating pathogenic factors. Insufficient righteousness can effectively resist the occurrence of diseases, eliminate the harm of pathogenic factors to the human body and facilitate recovery. Modern medicine has found that after the hepatitis virus invades the human body, it replicates in large quantities in the liver and continuously destroys liver cells. The main clinical manifestation is a sharp increase in alanine aminotransferase. A large number of animal experiments and clinical experiments prove that the pharmaceutical composition of the invention has the effect of significantly reducing alanine aminotransferase, can protect the liver from poison (CCl ₄ ) damage, and hardly promote the repair function of liver cells after acute injury.

Rheumatoid arthritis belongs to the category of "arthralgia" and "calendar disease" in traditional Chinese medicine. Traditional Chinese medicine believes that "arthralgia is closed, and the energy of Qi and blood as evil can be closed and painful." The occurrence of arthralgia is not only the feeling of rheumatism evil, but also the strength of the body plays an important role. "Qi Sheng Fang" clearly pointed out that "all are due to physical weakness, sparse interstitial space, and numbness caused by wind, cold and dampness." Due to the deficiency of qi, the deficiency of yang qi, the loss of Wei yang, the three evils of wind, cold, and dampness take advantage of the emptiness and enter, and flow through the channels, causing qi and blood to block. Therefore, "strengthening the body and eliminating pathogens" is the basic method for treating arthralgia.

The significant effect of the pharmaceutical composition of the present invention on "strengthening the body and eliminating pathogenic factors" has been described above. Pharmacological experiments have also confirmed that it has obvious effects in anti-inflammation, detumescence, and analgesia, and has definite curative effects on rheumatoid arthritis, senile osteoporosis, and periosteitis.

In terms of reproduction, the pharmaceutical composition comprising the termite population and its main nest bacterial garden of the present invention can increase the weight of the testes and ovaries to normal levels due to aging or infertility, and the stromal cells and spermatocytes in the seminiferous tubules The content of DNA and RNA in the cells increases, promotes cell division, adjusts the secretory function of infertile patients, and improves the function of the hypothalamus-pituitary-ovary (testis) axis. Make the kidney qi weak, the kidney does not store essence, transform qi, produce marrow and restore function, and effectively treat infertility.

The pharmaceutical composition of the present invention comprising the dried body of the termite main nest fungus containing termite populations has antitussive effect.

The pharmaceutical composition of the present invention can be made into various oral dosage forms according to methods known to those skilled in the art, such as being directly packed into capsules to make capsules, mixed with fillers and compressed into tablets, or made into suspensions. liquid etc.

The pharmaceutical composition of the present invention comprising termite population and its main nest fungus is used for the treatment of anti-inflammation, detumescence and analgesia, and the dosage is 0.5-3g/d; when it is used for protecting the liver and reducing glutamic acid The dosage is 0.5-3g/d. When used as cough medicine, the dosage is 0.25-3g/d; when used to treat infertility, the dosage is 0.5-3g/d; when used as health care medicine, the dosage is 0.25-1g/d.

Animal tests show that the pharmaceutical composition of the present invention comprising termite population and its main nest fungus is safe in use. In the acute toxicity test, due to the limited stomach capacity of mice, the oral LD50 cannot be obtained by gavage with 250mg/ml, and the maximum tolerated dose is 37.5g/kg, which is equivalent to 200-900 times the clinical dosage. The long-term toxicity test adopts 200 times (4g/kg), 400 times (8g/kg), 50 times and 100 times of the therapeutic dosage to carry out 12 week toxicity observations higher than the health dosage of clinical application, and its result shows, takes medicine of the present invention for a long time The composition does not damage the health status of animals, does not affect various indicators of hematology, and does not affect the functions of organs such as liver and kidney. It can be seen that the pharmaceutical composition of the present invention can be taken continuously for a long time, and is safe and reliable.

Nearly one hundred volunteers took the pharmaceutical composition of the present invention for 3 months to 5 years, and verified the efficacy of the following animal experiments, and no toxic side effects were found.

The present invention will be described in detail below through the examples, but the present invention is not limited.

Example 1

In the Chinese fir forest in Jurong County, Jiangsu Province, the black-winged soil termite living nest with the main nest at 1.5-3.0m was selected to cut off the ant tunnel for termite escape, so that most of the termite colony was in the fungus garden. Bring the fungus garden back to the room; select the main nest to be the yellow-brown fungus garden (containing termite population) with an incubator, set the temperature at about 80°C, dehydrate and dry the fungus garden; use a pulverizer to grind the dried fungus garden Crush, sieve through 80 meshes into powder, and obtain 1000 grams of dry powder; dry powder is sterilized at 100°C for 60 minutes, under sterile conditions, 500 grams of which are packaged and stored in a low-temperature dry environment, and the other 500 grams are packed into Capsules, 0.25g per capsule.

Example 2

In the Pinus massoniana forest in the suburbs of Nanning, Guangxi, select the live nest of the black-winged soil termite whose main nest is 0.5-1.0m, repeat the method of embodiment 1, and place 500 grams of products made in a low-temperature and dry environment after packaging for preservation. Another 500 grams of fillers were added and compressed into tablets each containing 0.25 g of the product of this embodiment.

Example 3

In the rubber forest of Xishuangbanna area, Yunnan Province, select the live nest of the black-winged soil termite whose main nest is 0.5-1.0m, repeat the method of embodiment 1, and place 500 grams of products made in a low-temperature and dry environment after packaging to preserve. Another 500 grams of fillers were added and compressed into tablets each containing 0.25 g of the product of this embodiment.

Example 4

In the eucalyptus forest of Leizhou Peninsula, Guangdong, select the live nest of the black winged termite whose main nest is 0.5-1.0m, repeat the method of embodiment 1, and place 500 grams of products made in a low-temperature and dry environment after packaging for preservation. Another 500 grams are packed into capsules, each containing 0.25 g of the product of this embodiment.

The following examples are used to illustrate the effects of the pharmaceutical composition of the present invention. Embodiment 5-8 illustrates its anti-inflammatory and analgesic effect; Experimental example 9 shows its hepatoprotective effect; Embodiment 10 shows its effect of improving cold tolerance; Embodiment 11 illustrates its therapeutic effect on infertility; Embodiment 12 illustrates Antitussive effect of the pharmaceutical composition of the present invention. Wherein, the pharmaceutical composition refers to a pharmaceutical composition comprising termite population and its main nest fungus.

In the following examples, the experimental animals used are all from the Experimental Animal Center of the Chinese Academy of Medical Sciences, and the drugs are all commercially available products.

The impact of embodiment 5 on rat formaldehyde arthritis

1. Drug preparation: The dry powder obtained in Example 1 is fully ground with 0.5% methylcellulose to form a suspension and placed in a refrigerator at 4°C for later use.

2. Materials and methods: Take 40 Wistar rats with a body weight of 160-180 grams (half and half for ♂♀), divide them into four groups, namely the control group and three dosage groups, 1.6 g/kg/day, 3.2 g/kg /day, 6.4 grams/kg/day, one hour after gavage on the first day of the experiment, 0.06ml of 2.5% formaldehyde solution was injected subcutaneously in the middle part of the left and right limbs of the rats to cause inflammation, and the circumference of the left and right foot joints was measured after 5 hours. Feed the medicine once a day on the 2nd to 5th day, re-measure the foot joint 1 hour after the medicine, and find out the difference between the circumference of the left and right foot joints, which indicates the degree of swelling (ie, the degree of inflammation).

3. Results: See Table 1 and Table 1

4. Conclusion: 6.4g/kg/d and 3.2g/kg/d groups have obvious anti-inflammation and detumescence effect on formaldehyde arthritis.

The influence of embodiment 6 on the inflammation caused by xylene in rat ear

Medicine preparation is with embodiment 5.

1. Materials and methods: 40 Wistar rats with a body weight of 160-180g (♂♀ in half) were divided into 4 groups, and the drugs prepared above were given 6.4g/kg/d, 3.2g/kg/d, and 1.6g respectively. /kg/d orally, once a day, 6 times in a row. The control group was fed an equal volume of 0.5% methylcellulose. 1.5 hours after the last administration, instill 0.1ml of xylene in the left ear of the rat to cause inflammation. After 15 minutes, use a puncher with a diameter of 9mm to take ear discs from the same part of the two ears of the rat and weigh them. The difference between the two ears Indicates the degree of inflammation.

2. Results: See Table 2 Table 2 drug dosage Number of animals (only) The difference between the two ears (X±SD) (mg) P value 0.5% methyl fiber 10 102.70±9.80 1.6g/kg/day 8 100.13±9.05 3.2g/kg/day 10 90.60±12.06 <0.05 6.4g/kg/day 12 63.67±7.15 <0.01

3. Conclusion: 6.4g/kg/d and 3.2g/kg/d groups have obvious anti-inflammation and detumescence effects on the inflammation caused by xylene in rat ears.

The influence of embodiment 7 on mouse ear inflammation caused by xylene

Medicine preparation is with embodiment 5.

1. Materials and methods: 30 Kunming mice, body weight 18-22g, ♂♀ in half, divided into 3 groups, were given the above-mentioned drug 3.2/kg/d and 1.6g/kg/d orally, every day Once, 5 consecutive times. The control group was fed an equal volume of 0.5% methylcellulose. 1.5 hours after the last administration, instill 0.05m1 of xylene in the left ear of the rat to cause inflammation. After 15 minutes, use a puncher with a diameter of 9mm to take ear discs from the same part of the two ears of the mouse and weigh them. The difference between the two ears Indicates the degree of inflammation.

2. Results: See Table 3

Table three drug dosage Number of animals (only) The difference between the two ears (X±SD) (mg) P value 0.5% methyl fiber 10 35.15±6.88 1.6g/kg/day 10 32.89±7.82 3.2g/kg/day 10 25.67±7.85 <0.05

3. Conclusion: gavage of the pharmaceutical composition of the present invention at 3.2 g/kg/d has obvious anti-inflammatory and detumescent effects on the inflammation caused by xylene in the ears of mice.

Example 8 Effects on the Writhing Response of Acetic Peritonitis in Mice

Medicine preparation is with embodiment 5.

1. Materials and methods: 40 Kunming mice, weighing 18-22 g, were randomly divided into 4 groups, with 10 mice in each group, half male and half male. Feed the medicine once a day for three times in total. Two hours after the third administration, 0.2 ml of 0.6% acetic acid was injected intraperitoneally to induce pain, and the number of writhing reactions of the mice within 15 minutes after administration of the chemical pain-inducing agent was recorded. And compared with the control group, the results are shown in Table 4.

Table four group Dosage (g/kg×d) number of animals Average times of writhing X±SD distilled water 0 10 29.1±13.1 The product of embodiment 1 6g/kg×3 10 ^★ 14.1±10.2 The product of embodiment 1 12g/kg×3 10 ^★★ 4.0±3.9 Indomethacin l0mg/kg×3 10 ^★★ 3.4±4.0 ^★ P<0.05 ^★★ P<0.001

2. Conclusion: two doses of the pharmaceutical composition of the present invention have significant analgesic effects. The analgesic effect of 12g/kg dose is the same as that of indomethacin.

Example 9

Medicine preparation is with embodiment 5.

1. Materials and methods: 30 Kunming mice, ♂♀ combined use, were divided into 3 groups, drug administration group, positive and negative control groups. The dosage of the administration group was 5g/kg/d, once a day for six consecutive days. In the afternoon of the day before the first administration and the afternoon of the fourth administration, CCl ₄ (40 μl/rat) was subcutaneously injected. The positive control group was also subcutaneously injected with CCl ₄ , but no medicine was given. On the 7th day of the experiment, blood was collected, and the activity of alanine aminotransferase was determined by Rei's method, and liver biopsy was performed.

2. Results:

(1) Alanine aminotransferase assay results are shown in Table 5

Table five drug dosage Number of animals (only) GPT activity (X±SD) (unit/100ml blood) P value positive control 10 150.8±42.2 Feeding group 10 52.9±24.3 <0.01 negative control 10 50.0±10.6

(2) Observation of liver slices: The centrilobular region of the liver of the positive control animals was severely damaged, and the livers of the drug-administered animals gradually repaired and were similar to those of the negative control.

3. Conclusion: through the section observation and the mensuration of transaminase activity, show: the pharmaceutical composition of the present invention has the effect of reducing alanine aminotransferase activity. Compared with the positive control group, the difference was significant, but close to the level of the negative control group. It can protect the liver from the damage of poison (CCl ₄ ), and promote the repair function of cells after acute injury.

The impact of embodiment 10 on the cold resistance of mice

1. Drug preparation:

The dry powder of Example 2 was ground, sieved with 200 mesh, and prepared into a 200 mg/ml suspension with 0.5% methylcellulose.

2. Materials and methods:

A total of 36 Kunming mice, male, weighing 18-22 g, were randomly divided into three groups: (1) control group; (2) 4 g/kg group; (3) 8 g/kg group. The prepared medicine was fed, once a day, 11 times in total.

Animals in the control group and the two experimental groups were put into large beakers respectively, placed in a low-temperature refrigerator at -25°C, frozen for 2 hours, and then taken out to count the number of dead and surviving animals in each group.

3. Results: See Table 6

Table six level Number of animals (only) -25℃ for 2 hours P value Survival number (only) Number of deaths (only) mortality rate(%) control group 12 2 10 83.3 4g/kg group 12 7 5 41.7 <0.05 8g/kg group 12 9 3 25 <0.01

4. Conclusion: under the same conditions, each group of animal survival numbers is different, and through statistical processing, the experimental group and the matched group have shown differences, and the 8g/kg group is compared with the matched group, and the difference is very significant (P＜0.01), showing that the present invention The pharmaceutical composition does have the function of enhancing physical fitness.

Embodiment 11 to the clinical trial of treatment infertility

20 cases of women of childbearing age, 15 cases of primary infertility, 5 cases of secondary infertility. The main causes of infertility are menstrual disorders, basal body temperature (BBT) curves showing that the luteal phase is less than 12 days, and poor follicular development. After taking the capsule of Example 1 at 1 g/d for 90 days, menstrual disorders were improved, the BBT curve was biphasic, the luteal phase was greater than 13 days, and the follicle developed to more than 1.8 cm, and luteinizing hormone (LH) peak occurred during ovulation, local Conditions: CM increases and the traits are good. After 2-3 courses of treatment (30 days per course, 1g per day), the symptoms improved relatively satisfactorily. 17 cases showed significant results, and 3 of them became pregnant.

10 male cases were all primary infertility, 3 cases of oligospermia, 5 cases of semen volume less than 1.0ml; The effect, especially for those with low semen volume, can reach more than 2.0ml after 3 months.

Example 12 Antitussive effect

Case 1: A female, 56 years old, had a sudden irritating cough, which lasted for several months every year, accompanied by pain in the waist and legs, and various treatments were ineffective. After the attack, take 2 capsules of embodiment 1 every day 2 times, and the cough takes a turn for the better on the third day. After taking 100 capsules, there is no recurrence of cough in the past year, and the pain in the waist and legs has also been cured.

Case 2: A female, 39 years old, had dry cough without phlegm, which occurred every year. Taking traditional Chinese medicine and various anti-inflammatory western medicines did not work, and the attacks lasted for several months every year. After taking the capsules (1-2 capsules/day) of embodiment 1 in a variable amount, it took effect within a week, and there was no recurrence after drug withdrawal.

Claims (10)

1, a kind of pharmaceutical composition of the dry body that includes the termite nest fungus comb that contains termite population is used in preparation treatment mammal comprises the medicine of human chronic viral hepatitis, it is characterized in that described fungus garden dry body is the termite nest fungus comb dewatered drying that contains termite population that will dig out, pulverizing then, decontamination, sterilization and make, wherein said Coptotermes formosanus Shtrari. is an Odontotermes formosanus Shiraki, and described termitarium fungus garden is chicken _ bacterium.

2, application as claimed in claim 1 is characterized in that: the weight ratio of Coptotermes formosanus Shtrari. and termite nest fungus comb dry body is 1 in the described pharmaceutical composition: 15-20.

3, a kind of pharmaceutical composition of the dry body that includes the termite nest fungus comb that contains termite population comprises application in the medicine of human antiinflammatory, detumescence, treatment of arthritis the preparation mammal, it is characterized in that described fungus garden dry body is the termite nest fungus comb dewatered drying that contains termite population that will dig out, pulverizing then, decontamination, sterilization and make, wherein said Coptotermes formosanus Shtrari. is an Odontotermes formosanus Shiraki, and described termitarium fungus garden is chicken _ bacterium.

4, application as claimed in claim 3 is characterized in that: the weight ratio of Coptotermes formosanus Shtrari. and termite nest fungus comb dry body is 1 in the described pharmaceutical composition: 15-20.

5, a kind of pharmaceutical composition of the dry body that includes the termite nest fungus comb that contains termite population improves application in the medicine that mammal comprises the human body tolerance to cold in preparation, it is characterized in that described fungus garden dry body is the termite nest fungus comb dewatered drying that contains termite population that will dig out, pulverizing then, decontamination, sterilization and make, wherein said Coptotermes formosanus Shtrari. is an Odontotermes formosanus Shiraki, and described termitarium fungus garden is chicken _ bacterium.

6, application as claimed in claim 5 is characterized in that: the weight ratio of Coptotermes formosanus Shtrari. and termite nest fungus comb dry body is 1 in the described pharmaceutical composition: 15-20.

7, a kind of pharmaceutical composition of the dry body that includes the termite nest fungus comb that contains termite population comprises application in the medicine of human infertility preparation treatment mammal, it is characterized in that described fungus garden dry body is the termite nest fungus comb dewatered drying that contains termite population that will dig out, pulverizing then, decontamination, sterilization and make, wherein said Coptotermes formosanus Shtrari. is an Odontotermes formosanus Shiraki, and described termitarium fungus garden is chicken _ bacterium.

8, application as claimed in claim 7 is characterized in that: the weight ratio of Coptotermes formosanus Shtrari. and termite nest fungus comb dry body is 1 in the described pharmaceutical composition: 15-20.

9, a kind of pharmaceutical composition of the dry body that includes the termite nest fungus comb that contains termite population comprises application in the medicine of human cough-relieving the preparation mammal, it is characterized in that described fungus garden dry body is the termite nest fungus comb dewatered drying that contains termite population that will dig out, pulverizing then, decontamination, sterilization and make, wherein said Coptotermes formosanus Shtrari. is an Odontotermes formosanus Shiraki, and described termitarium fungus garden is chicken _ bacterium.

10, application as claimed in claim 9 is characterized in that: the weight ratio of Coptotermes formosanus Shtrari. and termite nest fungus comb dry body is 1 in the described pharmaceutical composition: 15-20.