[go: up one dir, main page]

CN102940157B - Traditional Chinese medicine feed additive for improving disease resistance of grass carp - Google Patents

Traditional Chinese medicine feed additive for improving disease resistance of grass carp Download PDF

Info

Publication number
CN102940157B
CN102940157B CN201210518481.6A CN201210518481A CN102940157B CN 102940157 B CN102940157 B CN 102940157B CN 201210518481 A CN201210518481 A CN 201210518481A CN 102940157 B CN102940157 B CN 102940157B
Authority
CN
China
Prior art keywords
weight portions
weight portion
weight
additive
feed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201210518481.6A
Other languages
Chinese (zh)
Other versions
CN102940157A (en
Inventor
吕明斌
燕磊
唐婷婷
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Huanggang new hope feed Technology Co., Ltd.
Original Assignee
Shandong New Hope Liuhe Group Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shandong New Hope Liuhe Group Co Ltd filed Critical Shandong New Hope Liuhe Group Co Ltd
Priority to CN201210518481.6A priority Critical patent/CN102940157B/en
Publication of CN102940157A publication Critical patent/CN102940157A/en
Application granted granted Critical
Publication of CN102940157B publication Critical patent/CN102940157B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Landscapes

  • Medicines Containing Plant Substances (AREA)
  • Fodder In General (AREA)

Abstract

The invention provides a feed for improving the disease resistance of a grass carp. The feed is characterized by comprising soybean cake, wheat meal, shell meal, fish meal, table salt, rice bran, fish oil, betaine, traditional Chinese medicine feed premix additive, zinc-containing polypeptide, zinc-containing microelement additive, vitamin mixture additive at least containing vitamin C, vitamin E and inositol, yeast and amino acid mixture additive at least containing threonine. The feed provided by the invention can improve the immunity of the organism of the grass carp and protect the grass carp from getting sick; the application of antibacterial chemical medicines and anti-virus chemical medicines is reduced to a certain degree, the survival rate of the grass carp is increased, and the activity of lysozyme and superoxide dismutase in the grass carp is improved; and moreover, the feed ensures little drug residue and low toxicity, and does not influence the quality of the grass carp or harm the human health.

Description

For improving the Chinese herbal feed additive of grass carp resistance against diseases
Technical field
The present invention relates to technical field of aquaculture, relate in particular to a kind of for improving the Chinese herbal feed additive of grass carp resistance against diseases.
Background technology
Grass carp ( ctenopharyngodon idellus), belong to Cyprinidae, Leuciscinae, grass carp genus, be commonly called as careless grass carp, grass roots (northeast), one of China famous " four large Chinese carps ", deeply being subject to people's favor, is that people like best edible a kind of marine product, and grass carp aquaculture is the mariculture industry of China's maximum.
But in four large Chinese carps, grass carp is the most liable to illness, be mainly to suffer from enteritis, red skin etc., to grass carp cultivation, brought very large difficulty, in addition, hemorrhage be also grass carp be very easy to suffer from by a kind of communicable disease of exhaling intestines guanidine virus to cause, in breeding process, this disease of easy infection all from fingerling to adult fish, this disease incidence of disease is high, the death rate is very large, causes great economic loss to professional raising farmer every year.
In order to treat the various diseases of grass carp, raiser uses antibiotic and chemicals in a large number, cause in animal body residual, impact and worsen product quality, serious harm health.
In order to improve the level of production of grass carp aquaculture, prevent that disease from bringing the tremendous economic loss of grass carp aquaculture, should be contained from source, by improving specific immunity ability and the non-specific immunity of grass carp, thereby be improved its opposing and prophylactic ability.
That Chinese medicine has is natural, efficient, toxic and side effect is little, the resistance to the action of a drug not significantly, aboundresources and the advantage such as performance is various, it has unique effect aspect prevent disease, manyly studies confirm that multiple Chinese herbal medicine all can affect aquatic livestock vivo oxidation enzyme activity, phagocyte vigor and Bacteriolytic activity, thus enhanced machine body immunity function.
Chinese herbal medicine contains the materials such as a large amount of flavones, alkaloid, organic acid and immune polysaccharide, can improve Immune Function In Animals, promotes interferon, lysozyme and the content of superoxide dismutase in serum, is the sign of animal disease resistant diseases prevention ability.Use Chinese herbal medicine can reduce the use of chemical classes medicine, there are explicit stipulation a lot of chemical classes medicines of inaccurate use in aquaculture of aquatic animal countries in the world recently, widely popularize organic cultivation, in aquaculture with Chinese herbal medicine instead of chemical synthetic as chloramphenicol etc., will there is very wide prospect.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of feed for improving the Chinese herbal feed additive of grass carp resistance against diseases and containing this feed addictive, by this feed of feeding, can improve the immunity of organisms of grass carp, prevent that grass carp is ill, reduce to a certain extent the use of antibacterium chemical drugs and antiviral chemical drugs, improve grass carp survival rate, improve the vigor of the interior lysozyme of grass carp body and superoxide dismutase, and this feed medicament residue rate is low, toxicity is low, can not affect the quality of grass carp, can not be detrimental to health.
In order to solve the problems of the technologies described above, the invention provides a kind ofly for improving the feed of grass carp resistance against diseases, it comprises soya-bean cake, wheat flour, oyster shell whiting, fish meal, salt, rice bran, fish oil, betaine, Chinese medicine fodder premix material additive, containing zinc polypeptide, iron content trace mineral supplement, the vitamin mixtures additive that at least contains vitamin C, vitamin E and inositol, yeast, the ispol additive that at least contains threonine.
Wherein, in described feed the percentage by weight of each component be soya-bean cake 5% ~ 9%, wheat flour 30% ~ 36%, oyster shell whiting 3% ~ 6%, fish meal 4% ~ 7%, salt 1% ~ 2%, rice bran 13% ~ 18%, fish oil 2% ~ 5%, betaine 1.5% ~ 2.5%, Chinese medicine fodder premix material additive 15% ~ 18%, containing zinc polypeptide 0.1% ~ 0.3%, iron content trace mineral supplement 0.2% ~ 0.3%, the vitamin mixtures additive 2% ~ 5% that at least contains vitamin C, vitamin E and inositol, yeast 2% ~ 4%, the ispol additive 2% ~ 5% that at least contains threonine.
Wherein, in described feed the percentage by weight of each component be soya-bean cake 7%, wheat flour 33%, oyster shell whiting 4.1%, fish meal 5%, salt 1.5%, rice bran 16%, fish oil 3%, betaine 2%, Chinese medicine fodder premix material additive 17%, containing zinc polypeptide 0.2%, iron content trace mineral supplement 0.2%, the vitamin mixtures additive 4% that at least contains vitamin C, vitamin E and inositol, yeast 3%, the ispol additive 4% that at least contains threonine.
Wherein, described Chinese herbal feed additive comprises following bulk drug: motherwort, holly leaf, tinosporae, prepared rhizome of rehmannia, dark plum, granatum, Herba Andrographitis, azedarach, dendrobium candidum, the Radix Astragali, scorpio, the vine of multiflower knotweed, Kaempferia galanga, the fruit of glossy privet, radix semiaquilegiae, arillus longan, folium cortex eucommiae.
Wherein, in described Chinese herbal feed additive, the weight portion of each bulk drug is motherwort 21 weight portion ~ 33 weight portions, holly leaf 26 weight portion ~ 39 weight portions, tinosporae 17 weight portion ~ 24 weight portions, prepared rhizome of rehmannia 39 weight portion ~ 52 weight portions, dark plum 10 weight portion ~ 18 weight portions, granatum 13 weight portion ~ 22 weight portions, Herba Andrographitis 20 weight portion ~ 28 weight portions, azedarach 16 weight portion ~ 24 weight portions, dendrobium candidum 37 weight portion ~ 55 weight portions, the Radix Astragali 79 weight portion ~ 88 weight portions, scorpio 30 weight portion ~ 36 weight portions, the vine of multiflower knotweed 47 weight portion ~ 58 weight portions, Kaempferia galanga 11 weight portion ~ 19 weight portions, the fruit of glossy privet 49 weight portion ~ 61 weight portions, radix semiaquilegiae 15 weight portion ~ 22 weight portions, arillus longan 38 weight portion ~ 46 weight portions, folium cortex eucommiae 46 weight portion ~ 53 weight portions.
Wherein, in described Chinese herbal feed additive, the weight portion of each bulk drug is motherwort 27 weight portions, holly leaf 32 weight portions, tinosporae 21 weight portions, prepared rhizome of rehmannia 45 weight portions, dark plum 14 weight portions, granatum 18 weight portions, Herba Andrographitis 24 weight portions, azedarach 20 weight portions, dendrobium candidum 46 weight portions, the Radix Astragali 84 weight portions, scorpio 33 weight portions, the vine of multiflower knotweed 53 weight portions, Kaempferia galanga 15 weight portions, the fruit of glossy privet 55 weight portions, radix semiaquilegiae 19 weight portions, arillus longan 42 weight portions, folium cortex eucommiae 50 weight portions.
The present invention also provides the preparation method of above-mentioned feed, and it is specially:
The first step, each bulk drug component in above-mentioned Chinese herbal feed additive is ground to form respectively to fine powder, mix, obtain mixture, mixture is joined in the distilled water of 6 ~ 8 times of amounts and decoct, each decoction 2 ~ 4 hours, decoct 3 times, decocted rear filtration at every turn, filtrate is mixed, carry out reduced pressure concentration and obtain medicinal extract, medicinal extract is joined in the ethanolic solution that the determining alcohol with respect to 3 ~ 5 times of amounts of mixture quality is 90% ~ 95%, stir completely and dissolve, subsequently 4 ℃ of following standing over night of temperature, subsequent filtration, filtrate is carried out to reduced pressure concentration and remove ethanol, the lotion obtaining is pulverized, and add starch according to the ratio of mass ratio 1:3 ~ 5, mix, with 14 mesh sieves, granulate, in 60 ℃ ~ 70 ℃ baking ovens, toast 30 minutes, obtain Chinese herbal feed additive,
Second step, it is even that the Chinese herbal feed additive that the first step is obtained and other each components in described feed are sent into stirrer for mixing in proportion, sends in pulverizer and pulverize subsequently, crosses 80～100 object screen clothes;
The 3rd step; material after pulverizing is sent in modem; pass into the steam that accounts for quality of material 15%～20%; the temperature modulated of 90～100 ℃ 6～7 minutes; then the modulation products of acquisition is entered at the temperature of 95～100 ℃ to extrusion granulator and granulate, under 90～103 ℃ of conditions, the feed granules after expanded is dried.
The present invention also provides for improving the Chinese herbal feed additive of grass carp resistance against diseases, and it comprises following bulk drug: motherwort, holly leaf, tinosporae, prepared rhizome of rehmannia, dark plum, granatum, Herba Andrographitis, azedarach, dendrobium candidum, the Radix Astragali, scorpio, the vine of multiflower knotweed, Kaempferia galanga, the fruit of glossy privet, radix semiaquilegiae, arillus longan, folium cortex eucommiae.
Wherein, the parts by weight of described each bulk drug are motherwort 21 weight portion ~ 33 weight portions, holly leaf 26 weight portion ~ 39 weight portions, tinosporae 17 weight portion ~ 24 weight portions, prepared rhizome of rehmannia 39 weight portion ~ 52 weight portions, dark plum 10 weight portion ~ 18 weight portions, granatum 13 weight portion ~ 22 weight portions, Herba Andrographitis 20 weight portion ~ 28 weight portions, azedarach 16 weight portion ~ 24 weight portions, dendrobium candidum 37 weight portion ~ 55 weight portions, the Radix Astragali 79 weight portion ~ 88 weight portions, scorpio 30 weight portion ~ 36 weight portions, the vine of multiflower knotweed 47 weight portion ~ 58 weight portions, Kaempferia galanga 11 weight portion ~ 19 weight portions, the fruit of glossy privet 49 weight portion ~ 61 weight portions, radix semiaquilegiae 15 weight portion ~ 22 weight portions, arillus longan 38 weight portion ~ 46 weight portions, folium cortex eucommiae 46 weight portion ~ 53 weight portions.
The present invention also provides the preparation method of above-mentioned Chinese herbal feed additive, and it is specially:
Above-mentioned raw materials medicine component is ground to form respectively to fine powder, mix, obtain mixture, mixture is joined in the distilled water of 6 ~ 8 times of amounts and decoct, each decoction 2 ~ 4 hours, decoct 3 times, decocted rear filtration at every turn, filtrate is mixed, carry out reduced pressure concentration and obtain medicinal extract, medicinal extract is joined in the ethanolic solution that the determining alcohol with respect to 3 ~ 5 times of amounts of mixture quality is 90% ~ 95%, stir completely and dissolve, subsequently 4 ℃ of following standing over night of temperature, subsequent filtration, filtrate is carried out to reduced pressure concentration and remove ethanol, the lotion obtaining is pulverized, and add starch according to the ratio of mass ratio 1:3 ~ 5, mix, with 14 mesh sieves, granulate, in 60 ℃ ~ 70 ℃ baking ovens, toast 30 minutes, obtain Chinese herbal feed additive.
Beneficial effect of the present invention:
This feed provided by the invention, can improve the immunity of organisms of grass carp, prevent that grass carp is ill, reduce to a certain extent the use of antibacterium chemical drugs and antiviral chemical drugs, improve grass carp survival rate, improve the vigor of the interior lysozyme of grass carp body and superoxide dismutase, and this feed medicament residue rate is low, toxicity is low, can not affect the quality of grass carp, can not be detrimental to health.
The specific embodiment
The invention provides a kind ofly for improving the feed of grass carp resistance against diseases, it comprises soya-bean cake, wheat flour, oyster shell whiting, fish meal, salt, rice bran, fish oil, betaine, Chinese medicine fodder premix material additive, containing zinc polypeptide, iron content trace mineral supplement, the vitamin mixtures additive that at least contains vitamin C, vitamin E and inositol, yeast, the ispol additive that at least contains threonine.
In described feed, the percentage by weight of each component is soya-bean cake 5% ~ 9%, wheat flour 30% ~ 36%, oyster shell whiting 3% ~ 6%, fish meal 4% ~ 7%, salt 1% ~ 2%, rice bran 13% ~ 18%, fish oil 2% ~ 5%, betaine 1.5% ~ 2.5%, Chinese medicine fodder premix material additive 15% ~ 18%, containing zinc polypeptide 0.1% ~ 0.3%, iron content trace mineral supplement 0.2% ~ 0.3%, at least contain vitamin C, the vitamin mixtures additive 2% ~ 5% of vitamin E and inositol, yeast 2% ~ 4%, the ispol additive 2% ~ 5% that at least contains threonine, in described feed, the mass percent sum of each component is 100%.
In described feed the percentage by weight of each component most preferably be soya-bean cake 7%, wheat flour 33%, oyster shell whiting 4.1%, fish meal 5%, salt 1.5%, rice bran 16%, fish oil 3%, betaine 2%, Chinese medicine fodder premix material additive 17%, containing zinc polypeptide 0.2%, iron content trace mineral supplement 0.2%, the vitamin mixtures additive 4% that at least contains vitamin C, vitamin E and inositol, yeast 3%, the ispol additive 4% that at least contains threonine.
Preferred described feed only consists of said components.
Betaine has obvious metabolic activity, can improve the survival rate of grass carp, and meanwhile, the food calling power of betaine and palatability can attract grass carp to go to ingest.
Zinc is the necessary trace elements of most of biologies, has important physiological function, it is that many enzymes are (as carbonic anhydrase, carboxypeptidase, alkaline phosphatase, lactic dehydrogenase etc.) constituent or activator, in grass carp body, zinc participates in multiple metabolic process, comprise carbohydrate, lipid, synthetic and the degraded of protein and nucleic acid, scarce zinc can make the immune organ of animal as thymus gland, lymph node, there is atrophy in spleens etc., periphery lymphocyte reduces, T cell function weakens, thereby cause body immunity to reduce, supplementing zinc makes its functional recovery normal, in the present invention, adopting containing zinc polypeptide is Bacitracin Zinc premix, it is the pale yellow powder shape crystal that Tianjin nova animal pharmaceutical factory produces.
Iron is a kind of hematopoiesis element, it is the important composition composition of hemoglobin, iron has important function to immunity of organism mechanism, because iron is activated centre or the coenzyme of some enzyme in nucleic acid reductase and biological oxidation process (xanthine oxidase, succinate dehydrogenase etc.), iron deficiency can affect the activity of these enzymes, thereby affect the synthetic and lymphocytic propagation of T of DNA, periphery lymph T lymphocyte in blood quantity is reduced, affect thick liquid cell synthesizing immunoglobulin (Ig), make phagocyte activity impaired, cause lethality to reduce.Thereby leucocyte sterilizing ability reduces during iron deficiency, body is easily infected, metainfective hematoclasis accelerates, the body death rate raises, if the utilization of iron content deficiency or iron affects adversely in feed, may cause fish growth to slow down, premunition declines, and the iron content trace mineral supplement adding in the present invention is ironic citrate.
Vitamin C is one of fish and the necessary nutritional labeling of shrimps, and, mineral absorption synthetic with hormone, metabolism have substantial connection, and can strengthen immunity of organisms, prevention scurvy, protection capilary, promotes wound healing.
Described vitamin E is the important nutriment of aquatic livestock; its major function is to eliminate unnecessary free radical in body; protect fat-soluble cell membrane and unrighted acid not oxidized, by the function of promotion and opsonic immunity cell, improve the immunity of animal body.
Vitamin C and vitamin E, as important antioxidant, lay respectively at endochylema and the phospholipid bilayer of cell, for cell provides effective antioxidant system.The former can remove, and normal cell is movable and various stress descend produced free radical; the latter is protecting highly unsaturated fatty acid to avoid playing an important role in the peroxidating of free radical mediated; therefore, appropriate vitamin C and vitamin E can improve the immunity of aquatic animal, anti-oxidant and anti-stress ability.
Inositol can make the speed of growth of grass carp accelerate, and meanwhile, reduces the generation of grass carp disease.
Described vitamin mixtures additive also can be selected any vitamin premix additive that comprises vitamin C, vitamin E and inositol or add vitamin premix additive when adding vitamin C, vitamin E, described vitamin premix additive can be FV-102 vitamin compound premix (Zhongmu Industry Co.,Ltd's production), and it contains vitamin C is that 100g/kg, vitamin E are 50,000 IU/kg.
Threonine is the first limiting amino acids that IgG generates, and threonine lacks can Immunosuppression globulin and the lymphocytic generation of T, B.
Described ispol additive also can add other amino acid additives such as methionine, serine, lysine.
Described Chinese herbal feed additive comprises following bulk drug: motherwort, holly leaf, tinosporae, prepared rhizome of rehmannia, dark plum, granatum, Herba Andrographitis, azedarach, dendrobium candidum, the Radix Astragali, scorpio, the vine of multiflower knotweed, Kaempferia galanga, the fruit of glossy privet, radix semiaquilegiae, arillus longan, folium cortex eucommiae.
Described Chinese herbal feed additive preferably only consists of above-mentioned raw materials medicine.
In described Chinese herbal feed additive, the weight portion of each bulk drug can be motherwort 21 weight portion ~ 33 weight portions, holly leaf 26 weight portion ~ 39 weight portions, tinosporae 17 weight portion ~ 24 weight portions, prepared rhizome of rehmannia 39 weight portion ~ 52 weight portions, dark plum 10 weight portion ~ 18 weight portions, granatum 13 weight portion ~ 22 weight portions, Herba Andrographitis 20 weight portion ~ 28 weight portions, azedarach 16 weight portion ~ 24 weight portions, dendrobium candidum 37 weight portion ~ 55 weight portions, the Radix Astragali 79 weight portion ~ 88 weight portions, scorpio 30 weight portion ~ 36 weight portions, the vine of multiflower knotweed 47 weight portion ~ 58 weight portions, Kaempferia galanga 11 weight portion ~ 19 weight portions, the fruit of glossy privet 49 weight portion ~ 61 weight portions, radix semiaquilegiae 15 weight portion ~ 22 weight portions, arillus longan 38 weight portion ~ 46 weight portions, folium cortex eucommiae 46 weight portion ~ 53 weight portions.
In described Chinese herbal feed additive, the weight portion of each bulk drug most preferably is motherwort 27 weight portions, holly leaf 32 weight portions, tinosporae 21 weight portions, prepared rhizome of rehmannia 45 weight portions, dark plum 14 weight portions, granatum 18 weight portions, Herba Andrographitis 24 weight portions, azedarach 20 weight portions, dendrobium candidum 46 weight portions, the Radix Astragali 84 weight portions, scorpio 33 weight portions, the vine of multiflower knotweed 53 weight portions, Kaempferia galanga 15 weight portions, the fruit of glossy privet 55 weight portions, radix semiaquilegiae 19 weight portions, arillus longan 42 weight portions, folium cortex eucommiae 50 weight portions.
Azedarach can be removed grass carp vivotoxin, regulate metabolic function, prevents and treats early the generation of various parasites, bacteriosis and spreads.
Astragalus polyose in the Radix Astragali can obviously promote the generation of body antibody, can make the serum IgA of grass carp, the level of IgM, IgG improves, antibody secreting cell and T, B lymphocyte and NK cell obviously strengthen, and strengthen normal grass carp and immunosupress grass carp peritoneal macrophage number and function.
Chinese herbal feed additive of the present invention can stimulate grass carp body linked groups to produce lysozyme and the superoxide dismutase of higher vigor, lysozyme is the important nospecific immunity factors of many organisms, being the important component part of grass carp disease system of defense, has been the material base of engulfing.Superoxide dismutase is one of important antioxidase in living organism Antioxidant Enzyme Systems, as active oxygen radical scavenger, is delaying body aging and is preventing from playing a very important role aspect large biological molecule damage; Also there is antiviral and antibacterial action, Chinese herbal feed additive of the present invention is by stimulating grass carp to produce lysozyme and the superoxide dismutase of higher vigor, thereby improve specific immunity ability and the non-specific immunity of grass carp, and then improve the anti-virus ability of grass carp.
The present invention also provides the preparation method of above-mentioned Chinese herbal feed additive, and it is specially:
Above-mentioned Chinese medicine material medicine component is ground to form respectively to fine powder, mix, obtain mixture, mixture is joined in the distilled water of 6 ~ 8 times of amounts and decoct, each decoction 2 ~ 4 hours, decoct 3 times, decocted rear filtration at every turn, filtrate is mixed, carry out reduced pressure concentration and obtain medicinal extract, medicinal extract is joined in the ethanolic solution that the determining alcohol with respect to 3 ~ 5 times of amounts of mixture quality is 90% ~ 95%, stir completely and dissolve, subsequently 4 ℃ of following standing over night of temperature, subsequent filtration, filtrate is carried out to reduced pressure concentration and remove ethanol, the lotion obtaining is pulverized, and add starch according to the ratio of mass ratio 1:3 ~ 5, mix, with 14 mesh sieves, granulate, in 60 ℃ ~ 70 ℃ baking ovens, toast 30 minutes, obtain Chinese herbal feed additive.
The present invention also provides the preparation method of above-mentioned feed, and it is specially:
The first step, prepares described Chinese herbal feed additive according to the method described above;
Second step, it is even that the Chinese herbal feed additive that the first step is obtained and other each components in described feed are sent into stirrer for mixing in proportion, sends in pulverizer and pulverize subsequently, crosses 80～100 object screen clothes;
The 3rd step; material after pulverizing is sent in modem; pass into the steam that accounts for quality of material 15%～20%; the temperature modulated of 90～100 ℃ 6～7 minutes; then the modulation products of acquisition is entered at the temperature of 95～100 ℃ to extrusion granulator and granulate, under 90～103 ℃ of conditions, the feed granules after expanded is dried.
Below adopt embodiment to describe embodiments of the present invention in detail, to the present invention, how application technology means solve technical problem whereby, and the implementation procedure of reaching technique effect can fully understand and implement according to this.
Embodiment 1 Chinese herbal feed additive 1
By motherwort 27g, holly leaf 32g, tinosporae 21g, prepared rhizome of rehmannia 45g, dark plum 14g, granatum 18g, Herba Andrographitis 24g, azedarach 20g, dendrobium candidum 46g, Radix Astragali 84g, scorpio 33g, vine of multiflower knotweed 53g, Kaempferia galanga 15g, fruit of glossy privet 55g, radix semiaquilegiae 19g, arillus longan 42g, folium cortex eucommiae 50g grinds to form respectively fine powder, mix, obtain mixture, mixture is joined in the distilled water of 4kg and decoct, each decoction 4 hours, decoct 3 times, decocted rear filtration at every turn, filtrate is mixed, carry out reduced pressure concentration and obtain medicinal extract, medicinal extract is joined in the ethanolic solution that the determining alcohol of 3kg is 95%, stir completely and dissolve, subsequently 4 ℃ of following standing over night of temperature, subsequent filtration, filtrate is carried out to reduced pressure concentration and remove ethanol, the lotion obtaining is pulverized, add 3kg starch, mix, with 14 mesh sieves, granulate, in 70 ℃ of baking ovens, toast 30 minutes, obtain Chinese herbal feed additive 1.
Embodiment 2 Chinese herbal feed additives 2
By motherwort 33g, holly leaf 39g, tinosporae 24g, prepared rhizome of rehmannia 52g, dark plum 18g, granatum 22g, Herba Andrographitis 28g, azedarach 16 24g, dendrobium candidum 55g, Radix Astragali 88g, scorpio 36g, vine of multiflower knotweed 58g, Kaempferia galanga 19g, fruit of glossy privet 61g, radix semiaquilegiae 22g, arillus longan 46g, folium cortex eucommiae 53g grinds to form respectively fine powder, mix, obtain mixture, mixture is joined in the distilled water of 5kg and decoct, each decoction 4 hours, decoct 3 times, decocted rear filtration at every turn, filtrate is mixed, carry out reduced pressure concentration and obtain medicinal extract, medicinal extract is joined in the ethanolic solution that the determining alcohol of 3.5kg is 95%, stir completely and dissolve, subsequently 4 ℃ of following standing over night of temperature, subsequent filtration, filtrate is carried out to reduced pressure concentration and remove ethanol, the lotion obtaining is pulverized, add 3kg starch, mix, with 14 mesh sieves, granulate, in 70 ℃ of baking ovens, toast 30 minutes, obtain Chinese herbal feed additive 2.
Embodiment 3 Chinese herbal feed additives 3
By motherwort 21g, holly leaf 26g, tinosporae 17g, prepared rhizome of rehmannia 39g, dark plum 10g, granatum 13g, Herba Andrographitis 20g, azedarach 16g, dendrobium candidum 37g, Radix Astragali 79g, scorpio 30g, vine of multiflower knotweed 47g, Kaempferia galanga 11g, fruit of glossy privet 49g, radix semiaquilegiae 15g, arillus longan 38g, folium cortex eucommiae 46g grinds to form respectively fine powder, mix, obtain mixture, mixture is joined in the distilled water of 3.5kg and decoct, each decoction 4 hours, decoct 3 times, decocted rear filtration at every turn, filtrate is mixed, carry out reduced pressure concentration and obtain medicinal extract, medicinal extract is joined in the ethanolic solution that the determining alcohol of 2.5kg is 95%, stir completely and dissolve, subsequently 4 ℃ of following standing over night of temperature, subsequent filtration, filtrate is carried out to reduced pressure concentration and remove ethanol, the lotion obtaining is pulverized, add 3kg starch, mix, with 14 mesh sieves, granulate, in 70 ℃ of baking ovens, toast 30 minutes, obtain Chinese herbal feed additive 3.
Toxicity test
The initial body weight 58.75 ± 3.49g of two grass carps in age for test, physical health, specification is consistent.
Feeding and management
Test is raised inner at glass jar (0.54m * 0.26m * 0.45m) with fish.Before on-test, with basal feed, raised and train grass carp 7, to conform and test feed.After on-test, except normally throwing something and feeding every day feed, 8:00 starts to fill with Chinese herbal feed additive 1mL/ tail prepared by hello the present invention, every day 1 time.Glass jar 24 is uninterruptedly inflated.During cultivation, water temperature is 22 ℃～24 ℃, and pH value is 7.6 ± 0.5, and dissolved oxygen is 6.7～7.4mg/L.
Test method
In first trial test, 210 tail grass carps are divided into 7 groups at random, establish 3 repetitions for every group, each repeats 10 tails, between group, fish body weight is without significant difference (P > 0.05), 5 groups of Chinese herbal feed additives 1 that use the present invention to prepare wherein, be dissolved in distilled water and used, with the difference of 10 times, determine 5 concentration (2.5g/mL, 0.25g/ml, 0.025/ml, 0.0025g/ml, 0.00025g/ml), 96h is processed in test, through test of many times, and progressively dwindle concentration range, finally find out 100% lethasl concentration (g/ml) and maximal tolerable concentration (g/ml), and according to etc. contrast spacing method determine each test group concentration (concrete grammar: r=n-1
Figure 2012105184816100002DEST_PATH_IMAGE001
, in formula, r is adjacent two dose ratio, b is maximum dose level, a is lowest dose level, n is dosage group number), other two groups of Chinese herbal feed additive 2 and Chinese herbal feed additives 3 that use respectively the embodiment of the present invention 2 and embodiment 3 preparations, are dissolved in distilled water and are used, and concentration is 2.5g/mL.
Computational methods
Adopt karber method to calculate half lethal concentration LC 50computing formula LgLC 50=Xm-d (∑ p-0.5)
In formula, Xm is the common logarithm of dead group maximum dose; D is that adjacent set concentration common logarithm is poor; P is the death rate of each group; ∑ p is the summation of each group death rate.
Safe concentration computing formula
Sc＝?48hLC 50×0.3／(24hLC 50／48hLC 50) 2
Experimental result, in Table 1.
Table 1 Chinese herbal feed additive is to the death rate of grass carp, half lethal concentration and safe concentration
Figure 555310DEST_PATH_IMAGE002
From table 1 can, adopting Chinese herbal feed additive of the present invention is 0 at 24h and the equal death rate of 96h, according to formula, cannot calculate its half lethal concentration and safe concentration, but 24hLC 50necessarily be greater than 2.5g/mL, thereby can find out, Chinese herbal feed additive of the present invention is a kind of green non-poisonous feed addictive.
Embodiment 4 feeds 1
By 170g Chinese herbal feed additive 1, 70g soya-bean cake, 330g wheat flour, 41g oyster shell whiting (production of Rongcheng, Shandong fish meal Co., Ltd), 50g fish meal (production of Rongcheng, Shandong fish meal Co., Ltd), 15g salt, 160g rice bran, 30g fish oil, 20g betaine (production of Shandong De Gao Chemical Co., Ltd.), 2g Bacitracin Zinc premix (the pale yellow powder shape crystal that Tianjin nova animal pharmaceutical factory produces), 2g ironic citrate (production of Jinan Li Tian food additives Co., Ltd), 40gFV-102 vitamin compound premix (Zhongmu Industry Co.,Ltd's production), 30g yeast (production of Jinan Li Tian food additives Co., Ltd), 20g threonine (production of Jinan Long Tian amino acid additive Co., Ltd), 20g lysine (production of Jinan Long Tian amino acid additive Co., Ltd), send in pulverizer and pulverize subsequently, cross 100 object screen clothes, material after pulverizing is sent in modem, pass into the steam that accounts for quality of material 18%, the temperature modulated of 100 ℃ 7 minutes, then the modulation products of acquisition being entered at the temperature of 100 ℃ to extrusion granulator granulates, under 100 ℃ of conditions, the feed granules after expanded is dried, obtain feed 1.
Embodiment 5 feeds 2
By 170g Chinese herbal feed additive 2, 70g soya-bean cake, 330g wheat flour, 41g oyster shell whiting (production of Rongcheng, Shandong fish meal Co., Ltd), 50g fish meal (production of Rongcheng, Shandong fish meal Co., Ltd), 15g salt, 160g rice bran, 30g fish oil, 20g betaine (production of Shandong De Gao Chemical Co., Ltd.), 2g Bacitracin Zinc premix (the pale yellow powder shape crystal that Tianjin nova animal pharmaceutical factory produces), 2g ironic citrate (production of Jinan Li Tian food additives Co., Ltd), 40gFV-102 vitamin compound premix (Zhongmu Industry Co.,Ltd's production), 30g yeast (production of Jinan Li Tian food additives Co., Ltd), 20g threonine (production of Jinan Long Tian amino acid additive Co., Ltd), 20g lysine (production of Jinan Long Tian amino acid additive Co., Ltd), send in pulverizer and pulverize subsequently, cross 100 object screen clothes, material after pulverizing is sent in modem, pass into the steam that accounts for quality of material 18%, the temperature modulated of 100 ℃ 7 minutes, then the modulation products of acquisition being entered at the temperature of 100 ℃ to extrusion granulator granulates, under 100 ℃ of conditions, the feed granules after expanded is dried, obtain feed 2.
Embodiment 6 feeds 3
By 170g Chinese herbal feed additive 3, 70g soya-bean cake, 330g wheat flour, 41g oyster shell whiting (production of Rongcheng, Shandong fish meal Co., Ltd), 50g fish meal (production of Rongcheng, Shandong fish meal Co., Ltd), 15g salt, 160g rice bran, 30g fish oil, 20g betaine (production of Shandong De Gao Chemical Co., Ltd.), 2g Bacitracin Zinc premix (the pale yellow powder shape crystal that Tianjin nova animal pharmaceutical factory produces), 2g ironic citrate (production of Jinan Li Tian food additives Co., Ltd), 40gFV-102 vitamin compound premix (Zhongmu Industry Co.,Ltd's production), 30g yeast (production of Jinan Li Tian food additives Co., Ltd), 20g threonine (production of Jinan Long Tian amino acid additive Co., Ltd), 20g lysine (production of Jinan Long Tian amino acid additive Co., Ltd), send in pulverizer and pulverize subsequently, cross 100 object screen clothes, material after pulverizing is sent in modem, pass into the steam that accounts for quality of material 18%, the temperature modulated of 100 ℃ 7 minutes, then the modulation products of acquisition being entered at the temperature of 100 ℃ to extrusion granulator granulates, under 100 ℃ of conditions, the feed granules after expanded is dried, obtain feed 3.
Experiment
Animal used as test: test is juvenile fish with grass carp, scale of construction 79g ~ 85g/ tail (3600 tail), healthy strong, male and female all have, and are purchased from fishing ground, Jinan.
Experiment feed
Experimental group one to three feed 1 that respectively prepared by the feeding embodiment of the present invention 4 to embodiment 6 is to feed 3, the feed of control group feeding and the difference of experimental group are, use flavomycoin 20mg/kg to replace the Chinese herbal feed additive 1 of the present invention's use to Chinese herbal feed additive 3, every group contains juvenile fish 900 tails.
Feeding method
Be 7 days～August 12 March in 2010 date of test.Juvenile fish is tested after raising and train 7 d indoor.Pond crucian carp fish culture in pond, stocking density 1/10 L.Pool area 900 m 2(long 42 m * wide 22 m), dark 80 cm, underneath fine sand 10 cm, with bamboo close (water can pass through, fish can not by being standard) all separation block into 15 m 2little Chi; Omnidistance average 16 ℃ of pond water temperature (wherein summer 24～29 ℃, average 25.5 ℃), pH value 6.5 ± 0.2, water quality meets fishery standard.
Feed day, feeding volume weighed 3%～5% quantitative of fixed place and time throwing something and feeding by fry, and the 2 times/d that throws something and feeds, every 10 d dosage 1 time.The survival rate of investigation grass carp.
Fish is put to death, with buffer solution (pH value 7.5,0.25mol/ sucrose, 0.025mol/L Tris-HCL, 0.1mmol/L EDTA-2Na solution) rinse, then suck surface moisture with blotting paper, remove sclerotin part, leave the tissue homogenates such as heart, liver and sexual gland.Accurately take tissue 0.30 g, add the buffer solution ice bath homogenate of 9 times, the centrifugal 10～15min of 3 000 r/m in low-temperature and high-speed centrifuge, gets supernatant 2 mL and measures lysozyme and superoxide dismutase vigor.Assay method builds up with reference to Nanjing the kit description that biological study is produced.
Test data adopts means standard deviation (x ± s) to represent, two sample averages are relatively with t check, two sample constituent ratio χ 2check, P < 0.05 has conspicuousness for difference.
Result
The impact of Chinese herbal feed additive on grass carp survival rate, in Table 2.
The impact of table 2 Chinese herbal feed additive on grass carp survival rate
Figure DEST_PATH_IMAGE003
As can be seen from Table 2, the feed feeding grass carp that adopts the present invention to prepare, its ill possibility reduces, and survival rate is significantly higher than control group.
The impact of Chinese herbal feed additive on lysozyme and superoxide dismutase vigor, the results are shown in Table 3.
The impact of table 3 Chinese herbal feed additive on lysozyme and superoxide dismutase vigor
Figure 320747DEST_PATH_IMAGE004
As can be seen from Table 3, adopt the interior lysozyme of grass carp body and the superoxide dismutase level of Chinese herbal feed additive feeding of the present invention to significantly improve, thereby prove that it has better defence disease, antiviral and antibacterial ability, immune level is higher.
Each bulk drug pharmacology of described Chinese medicine composition is as follows:
Motherwort: bitter, pungent, cold nature, enters liver, pericardium channel, has promoting blood circulation for regulating menstruation, the effect of inducing diuresis to reduce edema.For irregular menstruation, dysmenorrhoea, through closing, lochiorrhagia, oedema oliguria; Acute nephritis edema, motherwort has cardiac stimulant, increases the effect of coronary flow and myocardial nutritional flow amount. and motherwort has slight excitation to the rabbit heart in place; Extrasomatic rabbit heart is first had to slight inhibition, and later slight excited, heavy dose suppresses. and its stachydrine can reducing heart rate. and leonurine has humidification to the convergent force low dose of isolated frog heart, and heavy dose presents inhibition.This kind of inhibition may be relevant with excited vagus.Experimental results show that: motherwort has remarkable increase coronary flow and the effect of reducing heart rate quite significantly.Motherwort 1ml/kg is to anesthetized dog intravenous injection, also there is increase coronary flow, reduce the effect of coronary resistance, reducing heart rate and minimizing cardiac output and the work done of left chamber. motherwort all has antagonism in various degree to animal models such as expeirmental myocardial ischemia, myocardial infarction or arrhythmia cordis.Motherwort the myocardial ischemia in rats of Induced By Isoprenaline is improved or recover ischemic cardiac electrograph, increase coronary flow, improve microcirculation, dog myocardial infarction that rabbit myocardial ischemia that effect, the motherwort formulation such as reducing heart rate bring out pituitrin and CAL cause; can make that its lesion degree alleviates, infarction size reduces, myocardial ultrastructure is protected.
Holly leaf: bitter, cool in nature, return liver, kidney channel, there is the effect of clearing heat and nourishing yin, the flat liver of kidney-nourishing, cure mainly tuberculosis hemoptysis, osteopyrexia and fever, the card such as have a dizzy spell.
Tinosporae: bitter, cold in nature, return lung, large intestine channel, have clearing heat and detoxicating, the effect of relieving sore throat and acesodyne, for abscess of throat, ulcer serious case of furuncle, has loose bowels, dysentery, card such as gastral cavity abdomen heat pain grade.
Prepared rhizome of rehmannia: taste is sweet, warm in nature, return liver, kidney channel, have enrich blood moist, the effect that benefit essence is filled out marrow, sallow for the deficiency of blood, dizzy palpitaition, irregular menstruation, collapse incessantly liver-kidney Yin deficiency, hectic fever night sweat, seminal emission impotence, infertility, irregular menstruation, metrostaxis, soreness and weakness of waist and knees, Hiccough and deaf, the head is dim-sighted, poliosis, quench one's thirst, constipation, the cards such as syndrome characterized by dyspnea of suffering from a deficiency of the kidney.
Dark plum: bitter, acid, puckery, warm in nature, enter liver, lung, spleen, large intestine channel, have and astringe the lung, puckery intestines, hemostasis, the effect of promoting the production of body fluid, is used for the treatment of protracted dysentery more than, damp-heat dysentery, has blood in stool, the card such as hematuria, snowslide, inhibited to multiple germs such as shigella dysenteriae, Escherichia coli, typhoid bacilluses.
Granatum: taste acid, puckery, warm in nature, enter large intestine, kidney channel, there is relieving diarrhea with astringents, the effect of hemostasis desinsection, for rushing down for a long time protracted dysentery, hematochezia, under uterine bleeding band, the cards such as abdominal pain due to enterositosis.
Herba Andrographitis: bitter, cold in nature, the thoughts of returning home, lung, large intestine, urinary bladder channel, have clearing heat and detoxicating, cool blood, detumescence, the effect of eliminating dampness, cures mainly cold, fever, abscess of throat, aphthae, pertussis labor is coughed, dysentery, heat is drenched puckery pain, the carbuncle sore that swells, the cards such as venomous snake bite.
Azedarach: latin name meliae Cortex, bitter, cold in nature, return liver, spleen, stomach warp, there is desinsection, treat tinea, for roundworm disease, enterobiasis, the cards such as abdominal pain due to enterositosis.
Dendrobium candidum: latin name dendorbii Officinalis Caulis, taste is sweet, and cold nature has the nourishing the stomach of promoting the production of body fluid; Nourishing Yin and clearing heat; Moistening lung kidney-nourishing; The effect of the strong waist of improving eyesight, cures mainly pyreticosis Tianjin wound, dry polydipsia, deficiency of stomach-Yin, food is few retches, and abnormal heat is not moved back after being ill, fire excess from yin deficiency, hectic fever due to yin labor heat, order is secretly not clear, and muscles and bones is withered soft, < < Compendium of Materia Medica > >: except gas under numbness, tonifying five zang organs consumptive disease is won thin, reinforcing yin essence benefit essence.Take for a long time, thick stomach, never sufficient in benefit, flat stomach Qi, longue meat, by skin heat symptoms caused by an exopathgen miliaria gas, a little less than pin knee aches cold numbness, determines intelligence except frightened, makes light of one's life by commiting suicide and prolongs life.Benefit gas heat extraction, strong sun, by skin wandering arthritis, cold for a long time in bone, kidney tonifying benefit power.Smelting heating spontaneous perspiration, plug in ulcer apocenosis.
The Radix Astragali: taste is sweet, warm in nature, enter spleen, lung channel, there is help spleen soil, elevate a turnable ladder yang-energy, strengthening exterior and reducing sweat, the effect of holder sore myogenic, cures mainly the weak anorexia and loose stool due to spleen-lung Qi deficiency, palpitation, the visceral organ ptosis of the sinking of qi of middle-jiao, Qi dysfunction in blood control uterine bleeding is had blood in stool, and rushes down for a long time prolapse of the anus; The spontaneous sweating of exterior deficiency, the cards such as insufficiency of vital energy and blood.
Scorpio: taste is pungent, property is flat, returns Liver Channel, has breath wind antispastic, dispersing pathogen accumulation, the effect of removing obstruction in channels to relieve pain, cures mainly child convulsion, tic spasm, middle air port is askew, hemiplegia, lockjaw, rheumatoid arthritis stubborn, migraine and general headache, sore, the cards such as scrofula.
The vine of multiflower knotweed: taste is sweet, property is flat, and the thoughts of returning home, Liver Channel, have nourishing blood and tranquilization, and the effect of dispelling wind and removing obstruction in the meridians, cures mainly insomnia and dreamful sleep, deficiency of blood bodily pain, arthralgia due to wind-dampness; The cards such as pruitus, the vine of multiflower knotweed can be invigorated blood circulation, stimulated the menstrual flow, hemangiectasis, stimulate circulation, lactogenesis detumescence.
Kaempferia galanga: taste is pungent, warm in nature, returns stomach warp, has in promoting the circulation of qi temperature, and the effect of the pain relieving that helps digestion, cures mainly chest diaphragm turgor, coldness and pain in the epigastrium, the diet card such as do not disappear.
The fruit of glossy privet: taste is sweet, bitter, is slightly cold, enters liver, kidney channel, has strong liver kidney-nourishing, and the effect of removing heat to brighten vision, is mainly used in treating liver-kidney Yin deficiency, and the head loses foster dizzy tinnitus, the symptoms such as yin asthenia generating intrinsic heat.The fruit of glossy privet can promote leucocyte, strengthens the effect of immunologic function, and staphylococcus aureus, shigella dysenteriae are had to stronger inhibitory action.Modern medicine study proves, the fruit of glossy privet includes ursolic acid, sweet mellow wine, glucose, fatty wet goods, has cardiac stimulant, diuresis and hepatoprotective effect, can make the leucocyte decline quantity causing because of chemotherapy raise, and staphylococcus aureus, Escherichia coli are had to inhibitory action.
Radix semiaquilegiae: taste is sweet, micro-hardship, micro-pungent, cold in nature, returns liver, spleen, urinary bladder channel, has clearing heat and detoxicating; Dispersing swelling and dissipating binds; The effect of promoting diuresis for stranguria.Cure mainly children's's heat frightened; Epilepsy; Carbuncle is swollen; Furunculosis; Acute mastitis; Scrofula; The skin sore of itching; Red eye, swell pain; Pharyngalgia; Snake bite and insect sting; Heat is drenched; The cards such as sand pouring.
Arillus longan: warm in nature, taste is sweet, has tonifying heart and spleen, the effect of nourishing blood and tranquilization, for the card such as insufficiency of vital energy and blood, palpitation and severe palpitation, forgetful insomnia, the deficiency of blood be sallow.
Folium cortex eucommiae: latin name eucommiae Folium, taste is micro-pungent, warm in nature, returns liver, kidney channel, has filling liver kidney, and strengthening the bones and muscles, cures mainly kidney deficiency and liver, has a dizzy spell, waist knee ache, the soft card that waits of muscles and bones impotence.
All above-mentioned these intellectual properties of primary enforcement, do not set restriction this new product of other forms of enforcement and/or new method.Those skilled in the art will utilize this important information, and foregoing is revised, to realize similar implementation status.But all modifications or transformation belong to the right of reservation based on new product of the present invention.
The above, be only preferred embodiment of the present invention, is not the present invention to be done to the restriction of other form, and any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the equivalent embodiment of equivalent variations.But every technical solution of the present invention content that do not depart from, any simple modification, equivalent variations and the remodeling above embodiment done according to technical spirit of the present invention, still belong to the protection domain of technical solution of the present invention.

Claims (7)

1. for improving the feed of grass carp resistance against diseases, it is characterized in that, comprising: soya-bean cake, wheat flour, oyster shell whiting, fish meal, salt, rice bran, fish oil, betaine, Chinese medicine fodder premix material additive, containing zinc polypeptide, iron content trace mineral supplement, the vitamin mixtures additive that at least contains vitamin C, vitamin E and inositol, yeast, the ispol additive that at least contains threonine;
Described Chinese herbal feed additive consists of following bulk drug: motherwort, holly leaf, tinosporae, prepared rhizome of rehmannia, dark plum, granatum, Herba Andrographitis, azedarach, dendrobium candidum, the Radix Astragali, scorpio, the vine of multiflower knotweed, Kaempferia galanga, the fruit of glossy privet, radix semiaquilegiae, arillus longan, folium cortex eucommiae;
In described Chinese herbal feed additive, the weight portion of each bulk drug is motherwort 21 weight portion ~ 33 weight portions, holly leaf 26 weight portion ~ 39 weight portions, tinosporae 17 weight portion ~ 24 weight portions, prepared rhizome of rehmannia 39 weight portion ~ 52 weight portions, dark plum 10 weight portion ~ 18 weight portions, granatum 13 weight portion ~ 22 weight portions, Herba Andrographitis 20 weight portion ~ 28 weight portions, azedarach 16 weight portion ~ 24 weight portions, dendrobium candidum 37 weight portion ~ 55 weight portions, the Radix Astragali 79 weight portion ~ 88 weight portions, scorpio 30 weight portion ~ 36 weight portions, the vine of multiflower knotweed 47 weight portion ~ 58 weight portions, Kaempferia galanga 11 weight portion ~ 19 weight portions, the fruit of glossy privet 49 weight portion ~ 61 weight portions, radix semiaquilegiae 15 weight portion ~ 22 weight portions, arillus longan 38 weight portion ~ 46 weight portions, folium cortex eucommiae 46 weight portion ~ 53 weight portions.
2. feed as claimed in claim 1, it is characterized in that: in described feed, the percentage by weight of each component is soya-bean cake 5% ~ 9%, wheat flour 30% ~ 36%, oyster shell whiting 3% ~ 6%, fish meal 4% ~ 7%, salt 1% ~ 2%, rice bran 13% ~ 18%, fish oil 2% ~ 5%, betaine 1.5% ~ 2.5%, Chinese medicine fodder premix material additive 15% ~ 18%, containing zinc polypeptide 0.1% ~ 0.3%, iron content trace mineral supplement 0.2% ~ 0.3%, at least contain vitamin C, the vitamin mixtures additive 2% ~ 5% of vitamin E and inositol, yeast 2% ~ 4%, the ispol additive 2% ~ 5% that at least contains threonine.
3. feed as claimed in claim 1 or 2, is characterized in that: in described feed the percentage by weight of each component be soya-bean cake 7%, wheat flour 33%, oyster shell whiting 4.1%, fish meal 5%, salt 1.5%, rice bran 16%, fish oil 3%, betaine 2%, Chinese medicine fodder premix material additive 17%, containing zinc polypeptide 0.2%, iron content trace mineral supplement 0.2%, the vitamin mixtures additive 4% that at least contains vitamin C, vitamin E and inositol, yeast 3%, the ispol additive 4% that at least contains threonine.
4. feed as claimed in claim 1 or 2, is characterized in that: in described Chinese herbal feed additive, the weight portion of each bulk drug is motherwort 27 weight portions, holly leaf 32 weight portions, tinosporae 21 weight portions, prepared rhizome of rehmannia 45 weight portions, dark plum 14 weight portions, granatum 18 weight portions, Herba Andrographitis 24 weight portions, azedarach 20 weight portions, dendrobium candidum 46 weight portions, the Radix Astragali 84 weight portions, scorpio 33 weight portions, the vine of multiflower knotweed 53 weight portions, Kaempferia galanga 15 weight portions, the fruit of glossy privet 55 weight portions, radix semiaquilegiae 19 weight portions, arillus longan 42 weight portions, folium cortex eucommiae 50 weight portions.
5. the preparation method of feed described in claim 1, is characterized in that, is specially:
The first step, each bulk drug component in above-mentioned Chinese herbal feed additive is ground to form respectively to fine powder, mix, obtain mixture, mixture is joined in the distilled water of 6 ~ 8 times of amounts and decoct, each decoction 2 ~ 4 hours, decoct 3 times, decocted rear filtration at every turn, filtrate is mixed, carry out reduced pressure concentration and obtain medicinal extract, medicinal extract is joined in the ethanolic solution that the determining alcohol with respect to 3 ~ 5 times of amounts of mixture quality is 90% ~ 95%, stir completely and dissolve, subsequently 4 ℃ of following standing over night of temperature, subsequent filtration, filtrate is carried out to reduced pressure concentration and remove ethanol, the lotion obtaining is pulverized, and add starch according to the ratio of mass ratio 1:3 ~ 5, mix, with 14 mesh sieves, granulate, in 60 ℃ ~ 70 ℃ baking ovens, toast 30 minutes, obtain Chinese herbal feed additive,
Second step, it is even that the Chinese herbal feed additive that the first step is obtained and other each components in described feed are sent into stirrer for mixing in proportion, sends in pulverizer and pulverize subsequently, crosses 80～100 object screen clothes;
The 3rd step; material after pulverizing is sent in quality-adjusting device; pass into the steam that accounts for quality of material 15%～20%; the temperature modulated of 90～100 ℃ 6～7 minutes; then the modulation products of acquisition is entered at the temperature of 95～100 ℃ to extrusion granulator and granulate, under 90～103 ℃ of conditions, the feed granules after expanded is dried.
6. for improving the Chinese herbal feed additive of grass carp resistance against diseases, it is characterized in that: by following bulk drug, formed: motherwort, holly leaf, tinosporae, prepared rhizome of rehmannia, dark plum, granatum, Herba Andrographitis, azedarach, dendrobium candidum, the Radix Astragali, scorpio, the vine of multiflower knotweed, Kaempferia galanga, the fruit of glossy privet, radix semiaquilegiae, arillus longan, folium cortex eucommiae;
The parts by weight of described each bulk drug are motherwort 21 weight portion ~ 33 weight portions, holly leaf 26 weight portion ~ 39 weight portions, tinosporae 17 weight portion ~ 24 weight portions, prepared rhizome of rehmannia 39 weight portion ~ 52 weight portions, dark plum 10 weight portion ~ 18 weight portions, granatum 13 weight portion ~ 22 weight portions, Herba Andrographitis 20 weight portion ~ 28 weight portions, azedarach 16 weight portion ~ 24 weight portions, dendrobium candidum 37 weight portion ~ 55 weight portions, the Radix Astragali 79 weight portion ~ 88 weight portions, scorpio 30 weight portion ~ 36 weight portions, the vine of multiflower knotweed 47 weight portion ~ 58 weight portions, Kaempferia galanga 11 weight portion ~ 19 weight portions, the fruit of glossy privet 49 weight portion ~ 61 weight portions, radix semiaquilegiae 15 weight portion ~ 22 weight portions, arillus longan 38 weight portion ~ 46 weight portions, folium cortex eucommiae 46 weight portion ~ 53 weight portions.
7. the preparation method of Chinese herbal feed additive claimed in claim 6, is characterized in that, is specially:
Above-mentioned raw materials medicine component is ground to form respectively to fine powder, mix, obtain mixture, mixture is joined in the distilled water of 6 ~ 8 times of amounts and decoct, each decoction 2 ~ 4 hours, decoct 3 times, decocted rear filtration at every turn, filtrate is mixed, carry out reduced pressure concentration and obtain medicinal extract, medicinal extract is joined in the ethanolic solution that the determining alcohol with respect to 3 ~ 5 times of amounts of mixture quality is 90% ~ 95%, stir completely and dissolve, subsequently 4 ℃ of following standing over night of temperature, subsequent filtration, filtrate is carried out to reduced pressure concentration and remove ethanol, the lotion obtaining is pulverized, and add starch according to the ratio of mass ratio 1:3 ~ 5, mix, with 14 mesh sieves, granulate, in 60 ℃ ~ 70 ℃ baking ovens, toast 30 minutes, obtain Chinese herbal feed additive.
CN201210518481.6A 2012-12-06 2012-12-06 Traditional Chinese medicine feed additive for improving disease resistance of grass carp Active CN102940157B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201210518481.6A CN102940157B (en) 2012-12-06 2012-12-06 Traditional Chinese medicine feed additive for improving disease resistance of grass carp

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201210518481.6A CN102940157B (en) 2012-12-06 2012-12-06 Traditional Chinese medicine feed additive for improving disease resistance of grass carp

Publications (2)

Publication Number Publication Date
CN102940157A CN102940157A (en) 2013-02-27
CN102940157B true CN102940157B (en) 2014-03-12

Family

ID=47723241

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201210518481.6A Active CN102940157B (en) 2012-12-06 2012-12-06 Traditional Chinese medicine feed additive for improving disease resistance of grass carp

Country Status (1)

Country Link
CN (1) CN102940157B (en)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104413272A (en) * 2013-08-21 2015-03-18 南通海辰蛋白科技有限公司 Medical fish soaking liquid
CN103478426B (en) * 2013-09-27 2014-12-10 苏州市阳澄湖现代农业产业园特种水产养殖有限公司 Crucian compound feed and preparation method thereof
CN103583928B (en) * 2013-12-02 2015-03-25 青岛百慧智业生物科技有限公司 Feed used for improving immune functions of grass carp
CN103734531B (en) * 2013-12-30 2016-01-20 天津市津宏发饲料有限公司 A kind of young grass carps feed
CN103734532B (en) * 2013-12-30 2017-02-08 青岛青知企业管理咨询有限公司 Feed for grass carps in breeding season
CN103749979B (en) * 2014-01-04 2015-12-30 江苏天福莱集团有限公司 A kind of method of supporting in process heavy metal of degrading aquatic livestock temporarily
CN103892051B (en) * 2014-04-16 2015-11-25 盐城工学院 A kind of Chinese herbal feed additive preventing and treating mullet hemorrhage
CN104054940B (en) * 2014-05-27 2016-05-18 范治淮 A kind of disease-resistant grass carp feed and preparation method thereof
CN104054937A (en) * 2014-05-27 2014-09-24 范治淮 Intake-increasing grass carp feed and preparation method thereof
CN104222553A (en) * 2014-08-15 2014-12-24 六安市信和生态农业开发有限公司 Nutritional feed for grass carp and preparation method thereof
CN104285873B (en) * 2014-10-28 2016-08-24 溧阳市金泉生态科技园有限公司 Ecological high yield circulation kind cultivation system and method
CN104323039A (en) * 2014-11-13 2015-02-04 张家港耕耘家庭农场有限公司 Chinese herbal medicine grass carp feed
CN104431490A (en) * 2014-11-26 2015-03-25 河南恒瑞源实业有限公司 Fish feed additive containing eucommia bark powder and preparation process of fish feed additive
CN104431488A (en) * 2014-11-26 2015-03-25 河南恒瑞源实业有限公司 Preparation process of grass carp feed additive containing eucommia bark powder
CN104489306A (en) * 2014-11-26 2015-04-08 河南恒瑞源实业有限公司 Eucommia ulmoides powder-containing aquatic product nutritious feed additive and preparation process thereof
CN104431489A (en) * 2014-11-26 2015-03-25 河南恒瑞源实业有限公司 Carp feed additive containing eucommia bark powder and preparation process of carp feed additive
CN105029108A (en) * 2015-08-31 2015-11-11 青岛海之源智能技术有限公司 Fish feed preventing forktonque goby canker and preparation method of fish feed
CN105558435A (en) * 2016-01-25 2016-05-11 浙江艾杰斯生物科技有限公司 Feed additive for preventing and treating silver carp hemorrhagic disease
CN106234847A (en) * 2016-08-08 2016-12-21 广西吉品环保科技有限公司 A kind of environment-friendly type feed of grass carp
CN106307592A (en) * 2016-08-12 2017-01-11 怀宁县龙盛草业有限公司 Grass carp feed rich in dietary fibers and preparation method of grass carp feed
CN106417999A (en) * 2016-09-18 2017-02-22 广西大学 A traditional Chinese medicine feed additive for improving immune function of grass carp and preparation method thereof
CN106511873A (en) * 2016-10-20 2017-03-22 南宁海世界生物科技有限公司 Traditional Chinese medicine feed additive and preparing method thereof
CN107348237A (en) * 2017-07-25 2017-11-17 明光市永言水产(集团)有限公司 A kind of feed for improving grass carp meat
CN107500377A (en) * 2017-10-13 2017-12-22 桂林融通科技有限公司 A kind of additive alleviated acidifying seawater and influenceed on big oyster lysozyme activity
CN107836598A (en) * 2017-11-10 2018-03-27 贵州省思南县山野牧业有限公司 A kind of feed addictive for treating hemorrhagic disease of grass carp and preparation method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102178096A (en) * 2011-04-26 2011-09-14 江苏省海之缘食品有限公司 Grass carp brittle granulated feed and preparation method
CN102366030A (en) * 2011-10-25 2012-03-07 陕西大秦汉集团有限公司 Mixed composite microbial feed for freshwater fish and production method thereof
CN102805273A (en) * 2012-08-31 2012-12-05 山东新希望六和集团有限公司 Fish feed for mixed culture and preparation method of fish feed

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH03219838A (en) * 1989-11-06 1991-09-27 Itochu Shiryo Kk Feed for reducing amount of accumulated body fat

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102178096A (en) * 2011-04-26 2011-09-14 江苏省海之缘食品有限公司 Grass carp brittle granulated feed and preparation method
CN102366030A (en) * 2011-10-25 2012-03-07 陕西大秦汉集团有限公司 Mixed composite microbial feed for freshwater fish and production method thereof
CN102805273A (en) * 2012-08-31 2012-12-05 山东新希望六和集团有限公司 Fish feed for mixed culture and preparation method of fish feed

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
JP平3-219838A 1991.09.27

Also Published As

Publication number Publication date
CN102940157A (en) 2013-02-27

Similar Documents

Publication Publication Date Title
CN102940157B (en) Traditional Chinese medicine feed additive for improving disease resistance of grass carp
CN102835609B (en) Pig feed capable of preventing piglets from diarrhea and traditional Chinese medicine composition
CN103355552B (en) Mixed feed for carp in fingerling stage and preparation method thereof
CN103689296B (en) Feed for ewe at later pregnancy and lactation period and preparation method thereof
CN103141710A (en) Compound feed for weaned pigs and preparation method of feed
CN103652361A (en) Feed for postpartum sow in lactation period of 1-20 days and preparation method thereof
CN104068279B (en) A kind of eel feed containing Chinese medicine additive and preparation method thereof
CN103719609B (en) Feed for lactating sows and preparation method thereof
CN103704513A (en) Feed for treating swine plague and preparation method thereof
CN104222638A (en) Functional feed for preventing and treating carp branchiomycosis and preparation method of functional feed
CN106259071A (en) A kind of natural cultural method of crab
CN104824455A (en) Cattle formula feed and preparation method thereof
CN102640876A (en) Feed and injection used for treating duck virus hepatitis and preparation method of feed and injection
CN105124276A (en) Feed for raising crabs
CN104206916A (en) Functional feed for treating ichthyoxeniosis of carps and preparation method of functional feed
CN104430179B (en) The cultural method of piglet
CN103931953A (en) Feed for preventing and controlling edema disease of swine, Chinese medicinal composition and preparation method
CN104351571A (en) Feed for preventing fish infectious hematopoietic necrosis and preparation method thereof
CN104366123A (en) Functional feed for preventing infectious pancreatic diseases in rainbow trouts and production method thereof
CN105146180A (en) Hairy crab compound feed
CN104256165A (en) Functional feed for preventing and treating chronic swine plague and preparation method of functional feed
CN105211527A (en) A kind of cultivation steamed crab feed and preparation method
CN103007111B (en) Chinese medicinal preparation for treating diarrhea of rabbits and preparation method of Chinese medicinal preparation
CN105661003A (en) Chinese herbal medicine feed additive capable of improving meat quality of fish bodies
CN1067555C (en) Application of medicinal combination containing termite population and main nest bacterial nursery

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
TR01 Transfer of patent right

Effective date of registration: 20170612

Address after: 438000, No. three nanhu road, Nanhu Nanhu Industrial Park, Huanggang, Hubei, Huanggang, 9

Patentee after: Huanggang new hope feed Technology Co., Ltd.

Address before: 266000 Shandong city of Qingdao province Chengyang Jihongtan Street Green Industrial Park

Patentee before: Shandong New Hope Liuhe Group Co., Ltd.

TR01 Transfer of patent right