CN107625889B - Plant extract spray for improving reproductive performance of cocks - Google Patents
Plant extract spray for improving reproductive performance of cocks Download PDFInfo
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Abstract
The invention discloses a plant extract spray for improving the reproductive performance of cocks, which is prepared by the following steps: firstly, selecting pine needles, perilla seeds, paulownia flowers, sealwort, Chinese chives seeds, epimedium herb and eurycoma longifolia, then cleaning, drying, mixing and crushing, adding boiling water and stirring into pasty Chinese medicinal powder, freezing and thawing, carrying out primary alcohol extraction, secondary alcohol extraction, tertiary alcohol extraction, combining filtrates, removing ethanol, adding drinking water, stirring and concentrating, and finally filling to obtain the traditional Chinese medicine composition. The plant extract spray improves the breeding performance of the cocks in a cloaca administration mode, and has the characteristics of environmental protection, small toxic and side effects, avoidance of damage to chicken livers and intestines and stomach, and continuous and stable improvement of the breeding performance of the cocks.
Description
Technical Field
The invention relates to the technical field of animal health care, in particular to the technical field of health care in the aspect of improving the production performance of breeding poultry.
Background
There are many factors that affect the reproductive performance of breeding cocks, such as genetic factors: the breeding performance of breeding cocks of different breed types has certain difference; week age factors: the reproductive performance of the breeding cocks is gradually reduced along with the increase of the week age; disease factors: any disease may cause physiological dysfunction, which may result in the inhibition of the growth process of sperm, and thus the quality of sperm. Such as salmonella, escherichia coli, marek's disease, etc.; nutritional factors: the quality of semen can be directly influenced by low protein content, insufficient heat and deficiency of vitamins and minerals in the feed; some drugs are improperly used, which can cause the reproductive performance of cocks to be reduced, such as sulfonamides, furazolidone, reserpine and the like; certain toxins in the feed: such as free gossypol in cottonseed meal, azolethione in rapeseed meal, mycotoxin in moldy feed, etc., can also directly or indirectly affect reproductive performance; environmental factors: ambient temperature: has great influence on the reproductive performance of the cock, such as temporary inhibition of cock sperms when the environmental temperature reaches 38 ℃. The sperm metabolism is accelerated and the sperm motility is reduced when the semen is stored in a high-temperature environment; management factors: if the semen collection technology is unskilled, the replacement of staffs is frequent, the breeding performance of the cocks is reduced due to too much labor and frequent semen collection times; unclean vas deferens or residual disinfectant is also harmful to sperm.
At present, the technical method for improving the reproductive performance of the cocks by the animal health care technology mainly adopts different technical measures to remove factors influencing the reproductive performance of the cocks from the aspects of diseases, stress resistance and the like of the cocks. If the disease is caused, corresponding western medicines or traditional Chinese medicines are added into the feed or the drinking water for treatment, and the technical means is mainly a drinking water or material mixing mode. The technical means sometimes have unobvious effects and sometimes are complicated to operate, and the methods need detoxification through the liver no matter drinking water or stirring materials, so that the increase of the fat content and the steatosis of the liver can be caused; causing cholestatic jaundice, hepatocyte necrosis, increase of glutamic-pyruvic transaminase, and even fatal fatty liver; increase of liver transaminase, hepatomegaly, and the like, thereby reducing the body resistance of the chicken. The periphery of the cloaca of the chicken is rich in arteries, veins and lymphatic plexus, and the cloaca mucosa has a strong absorption function. The cloacal administration can make the effective component quickly absorbed by cloacal mucosa and enter into the general circulation, so that it can exert its medicinal effect to cure systemic or local diseases. The drug is administered through the cloaca, the drug is mixed in the secretion of the cloaca and absorbed through the mucosa of the large intestine, and the drug does not pass through the liver, so that the first-pass detoxification effect of the liver is avoided, the blood concentration is improved, and the liver is not damaged; the medicine does not pass through the stomach and the small intestine, thereby avoiding the influence and damage of acid and alkali digestive enzyme on the medicine, relieving the stimulation of the medicine to the gastrointestinal tract, and greatly improving the bioavailability of the medicine by the chicken through cloacal administration.
Disclosure of Invention
An object of the present invention is to solve at least the above problems and to provide at least the advantages described later.
The invention also aims to provide a plant extract spray for improving the reproductive performance of cocks, which improves the reproductive performance of cocks in a cloaca administration mode and has the characteristics of environmental protection, small toxic and side effects, avoidance of damage to chicken livers and intestines and stomach, and continuous and stable improvement of the reproductive performance of cocks.
To achieve these objects and other advantages in accordance with the present invention, there is provided a herbal extract spray for improving reproductive performance of a rooster, prepared by the following process: firstly, selecting pine needles, perilla seeds, paulownia flowers, sealwort, Chinese chives seeds, epimedium herb and eurycoma longifolia, then cleaning, drying, mixing and crushing, adding boiling water and stirring into pasty medicinal powder, freezing and thawing, carrying out primary alcohol extraction, secondary alcohol extraction, tertiary alcohol extraction, combining filtrates, removing ethanol, adding drinking water, stirring and concentrating, and finally filling to obtain the traditional Chinese medicine composition.
Preferably, the plant medicinal materials comprise the following components in parts by weight: 40-50 parts of pine needle, 20-25 parts of perilla seed, 20-25 parts of paulownia flower, 10-15 parts of rhizoma polygonati, 10-15 parts of semen allii tuberosi, 2-6 parts of herba epimedii and 2-6 parts of eurycoma longifolia; the plant extract spray for improving the reproductive performance of cocks is prepared by comparing the effective components of the screened plant extracts according to the compatibility principles of traditional Chinese medicines such as monarch, minister, assistant and guide, seven-emotion compatibility and the like of compound plant medicines, repeatedly screening more than 200 plant medicines according to the design function requirements on the basis of a large amount of clinical practices, and has obvious effect of improving the reproductive performance of cocks and finally determining the formula. The pharmacological conditions of all plant medicines in the formula are as follows:
the folium Pini is needle leaf of Pinus sibirica, Pinus thunbergii, Pinus tabulaeformis, Pinus koraiensis, Pinus armandii, Pinus yunnanensis, Pinus sylvestris, Pinus massoniana, etc. of Pinus of Pinaceae, and has sour, bitter, warm, nontoxic, heart, spleen and liver meridians. It has effects in dispelling pathogenic wind, removing dampness, killing parasites, and relieving itching. The pine needles have the effects of treating diseases, preventing five internal organs, growing hair, resisting cold and summer heat, resisting wind and rain, lightening body, tonifying qi, keeping the middle warmer, preventing grains from prolonging life, removing pathogenic qi from pine leaves, keeping healthy and safe after removing pathogenic factors, preventing diseases from growing, and thus obtaining the actual effect of preventing hunger and prolonging life;
perilla seed, Perilla seed is a kind of Labiatae plant, Perilla seed, has slightly pungent taste, and has effects of lowering qi, eliminating phlegm, relieving asthma, moistening intestine, and can be used for treating phlegm stagnation and adverse flow of qi, cough and asthma, and constipation due to intestinal dryness;
flos Paulowniae is flower of Paulownia fortunei and Paulownia tomentosa of Scrophulariaceae, and has effects of clearing lung-heat, relieving sore throat, removing toxic substance and relieving swelling. It can be used for treating cough due to lung heat, acute tonsillitis, bacillary dysentery, acute enteritis, acute conjunctivitis, parotitis, furuncle, and skin sore and tinea;
sealwort, also known as: rhizoma Polygonati, herba Elsholtziae Pendulifoliae, rhizoma Gynurae Divaricatae, rhizoma Zingiberis recens, and radix Codonopsis Lanceolatae. Is a plant of Polygonatum, and has the effects of invigorating qi, nourishing yin, invigorating spleen, moistening lung, invigorating kidney, etc.;
semen allii tuberosi, alternative name: semen allii tuberosi and semen allii tuberosi, the efficacy classification: tonics, nature and flavor: pungent, sweet and warm in flavor, entering meridians: the liver channel and the kidney channel have the functions of tonifying the liver and the kidney, strengthening yang and securing essence, and are mainly used for treating: impotence due to kidney deficiency, soreness of the waist and knees, spermatorrhea, frequent micturition, turbid urine, and leukorrhagia;
semen allii tuberosi, alternative name: semen allii tuberosi and semen allii tuberosi, the efficacy classification: tonics, nature and flavor: pungent, sweet and warm in flavor, entering meridians: the liver channel and the kidney channel have the functions of tonifying the liver and the kidney, strengthening yang and securing essence, and are mainly used for treating: impotence due to kidney deficiency, soreness of the waist and knees, spermatorrhea, frequent micturition, turbid urine, and leukorrhagia;
eurycoma longifolia, a wild shrub plant growing in moist sandy soil in the original tropical rainforest near the equator in southeast Asia, namely, Maysia ginseng, Country ginseng, natural antacid and the like, is usually used as a medicine by root, and has the effect of promoting the growth of the prostate and seminal vesicle of animals.
Preferably, in all working links of the process preparation of the plant extract spray, except the freezing and thawing and stirring concentration links, the environment of other working links is 18-30 ℃, the relative humidity is 30-70%, and the cleanliness grade of a production area is a common production area.
Preferably, the pulverization of the plant medicinal material components is required to be pulverized into a powder having a particle size of 100 mesh.
Preferably, the freeze-thawing process of the powder is as follows: adding boiling water into the medicinal powder, stirring to obtain paste medicinal powder, freezing at-25 deg.C for 24 hr, and thawing at 25 deg.C.
Preferably, the alcohol extraction is carried out for the first time, the alcohol extraction is carried out for the second time, and the specific steps of the alcohol extraction for the third time are as follows: mixing the paste powder subjected to freeze thawing treatment with 95% ethanol solution at a mass ratio of 1:3, leaching at room temperature for 3h, vacuum filtering, performing repeated alcohol extraction for the second time and the third time, mixing the filtrates, and distilling under reduced pressure to remove ethanol.
Preferably, the steps of removing ethanol and adding drinking water for stirring and concentrating are as follows: adding 5 times volume of drinking water into the ethanol extractive solution, stirring and concentrating in a vacuum rotary instrument with water bath constant temperature of 60 deg.C, concentrating until the weight of the medicinal components is the same as that of the original plant medicinal materials, and stopping concentration.
Preferably, the plastic spray bottle with the volume of 100ml is selected for the filling process.
Preferably, the use method of the plant extract spray comprises the following steps: exposing the cloaca of the cock by turning the anus, spraying the liquid medicine to the exposed cloaca if inflammation such as red swelling, bleeding and the like, little semen collection amount and semen quality detection result shows dead semen and weak semen, continuously spraying the liquid medicine for three times every day, and continuously using for 3-5 days.
The invention at least comprises the following beneficial effects: (1) the pure Chinese medicinal preparation has mild medicinal properties and no irritation to chickens; (2) effectively treating the breeding performance of the cocks; (3) by cloacal administration, the medicine is absorbed through intestinal mucosa without damaging liver, thereby relieving the stimulation of the medicine to gastrointestinal tract, improving blood concentration and bioavailability of the medicine to chickens, and changing the traditional use method of the traditional Chinese medicine in the field of poultry production; (4) plant medicine is refined through freeze thawing and organic extraction technology, the extraction rate of active ingredients is remarkably improved, boiling water is selected for stirring medicine powder, the cell wall is broken again due to the principle of thermal expansion and cold contraction, in the freezing link, hot water freezes faster than cold water according to the principle of Mupeba effect, the speed of moisture in normal-temperature paste forming ice crystals is not as fast as the speed of moisture in high-temperature paste forming ice crystals, and the effect of breaking the wall is not good when high-temperature boiling water is used for stirring the medicine powder.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Detailed Description
The present invention is further described in detail below with reference to examples so that those skilled in the art can practice the invention with reference to the description.
< example 1>
A plant extract spray for improving the reproductive performance of cocks is prepared by the following steps: firstly, selecting pine needles, perilla seeds, paulownia flowers, sealwort, Chinese chives seeds, epimedium herb and eurycoma longifolia, then cleaning, drying, mixing and crushing, adding boiling water and stirring into pasty medicinal powder, freezing and thawing, carrying out primary alcohol extraction, secondary alcohol extraction, tertiary alcohol extraction, combining filtrates, removing ethanol, adding drinking water, stirring and concentrating, and finally filling to obtain the traditional Chinese medicine composition.
< example 2>
A plant extract spray for improving reproductive performance of cocks comprises the following medicinal materials in parts by weight: 50 parts of pine needle, 25 parts of perilla seed, 25 parts of paulownia flower, 15 parts of rhizoma polygonati, 15 parts of semen allii tuberosi, 6 parts of herba epimedii and 6 parts of eurycoma longifolia, and the selected medicinal materials are cleaned, dried and crushed into 100 meshes of medicinal powder. Mixing the pulverized medicinal powder with appropriate amount of boiling water, stirring to obtain paste, and freezing at-25 deg.C for 24 hr; freezing for 24 hr, taking out the pasty Chinese medicinal powder, and thawing at 25 deg.C. Mixing the pasty Chinese medicinal powder subjected to freeze thawing treatment with 95% ethanol solution at a mass ratio of 1:3, leaching at room temperature for 3h, vacuum filtering, repeating for 3 times (i.e. first ethanol extraction, second ethanol extraction, and third ethanol extraction), mixing filtrates, distilling under reduced pressure, volatilizing ethanol, adding 5 times volume of drinking water, stirring and concentrating on a vacuum rotary instrument with water bath constant temperature of 60 deg.C, concentrating to the same weight as the original medicinal material, and bottling in 100ml plastic spray bottle. In a chicken breeding farm of the Mingliu parent generation in 2016, 12 and 13 months, 58 cocks with red and swollen bleeding symptoms, less semen and poor semen quality in cloacae after turning anus are randomly selected and isolated in a cock breeding house, and are randomly divided into 2 groups of 29 cocks. After the whole test group turned the anus, the liquid medicine was sprayed to the cloaca for 3 times for 5 days, and the control group was treated by the conventional method for 5 days (anti-inflammatory agent was added to the feed, the same is applied to the following examples). After 5 days, the semen quality of 2 groups of cocks was examined, respectively, and the results were as follows:
TABLE 1 Effect of plant extract sprays on cock reproductive Performance
From the above results, the amount of sperm, sperm motility, sperm density, effective sperm count and sperm aberration rate of the test group in example 2 were all better than those of the control group, and the beneficial effects of the present invention were obtained.
< example 3>
A plant extract spray for improving reproductive performance of cocks comprises the following medicinal materials in parts by weight: 45 parts of pine needle, 23 parts of perilla seed, 23 parts of paulownia flower, 13 parts of rhizoma polygonati, 13 parts of semen allii tuberosi, 4 parts of herba epimedii and 4 parts of eurycoma longifolia, and the selected medicinal materials are cleaned, dried and crushed into 100 meshes of medicinal powder. Mixing the pulverized medicinal powder with appropriate amount of boiling water, stirring to obtain paste, and freezing at-25 deg.C for 24 hr; freezing for 24 hr, taking out the pasty Chinese medicinal powder, and thawing at 25 deg.C. Mixing the pasty Chinese medicinal powder subjected to freeze thawing treatment with 95% ethanol solution at a mass ratio of 1:3, leaching at room temperature for 3h, vacuum filtering, repeating for 3 times (i.e. first ethanol extraction, second ethanol extraction, and third ethanol extraction), mixing filtrates, distilling under reduced pressure, volatilizing ethanol, adding 5 times volume of drinking water, stirring and concentrating on a vacuum rotary instrument with water bath constant temperature of 60 deg.C, concentrating to the same weight as the original medicinal material, and bottling in 100ml plastic spray bottle. In a chicken breeding farm of the Mingliu parent generation in 2016, 12 and 28 days, a cock house is bred, 58 cocks with red and swollen bleeding symptoms, less semen and poor semen quality in cloacae after anus turning over are randomly selected and isolated, and the cocks are randomly divided into 2 groups of 29 chickens. After the whole test group turned the anus, the liquid medicine was sprayed to the cloaca for 3 times for 5 days, and the control group was treated by the conventional method for 5 days (anti-inflammatory agent was added to the feed, the same is applied to the following examples). After 5 days, the semen quality of 2 groups of cocks was examined, respectively, and the results were as follows:
TABLE 2 Effect of plant extract sprays on cock reproductive Performance
From the above results, it can be seen that the amount of sperm, sperm motility, sperm density, effective sperm count and sperm aberration rate of the test group in example 3 are all better than those of the control group, and the beneficial effects of the present invention are achieved.
< example 4>
A plant extract spray for improving reproductive performance of cocks comprises the following medicinal materials in parts by weight: 50 parts of pine needle, 25 parts of perilla seed, 25 parts of paulownia flower, 15 parts of rhizoma polygonati, 15 parts of semen allii tuberosi, 6 parts of herba epimedii and 6 parts of eurycoma longifolia, and the selected medicinal materials are cleaned, dried and crushed into 100 meshes of medicinal powder. Mixing the pulverized medicinal powder with appropriate amount of boiling water, stirring to obtain paste, and freezing at-25 deg.C for 24 hr; freezing for 24 hr, taking out the pasty Chinese medicinal powder, and thawing at 25 deg.C. Mixing the pasty Chinese medicinal powder subjected to freeze thawing treatment with 95% ethanol solution at a mass ratio of 1:3, leaching at room temperature for 3h, vacuum filtering, repeating for 3 times (i.e. first ethanol extraction, second ethanol extraction, and third ethanol extraction), mixing filtrates, distilling under reduced pressure, volatilizing ethanol, adding 5 times volume of drinking water, stirring and concentrating on a vacuum rotary instrument with water bath constant temperature of 60 deg.C, concentrating to the same weight as the original medicinal material, and bottling in 100ml plastic spray bottle. In 22.1.2017, in a brood cock farm of the Mingliu parent generation, 58 cocks with red and swollen bleeding symptoms, less semen and poor semen quality in cloaca after turning anus are randomly selected and isolated in a brood cock house, and are randomly divided into 2 groups of 29 cocks. After the whole test group overturns the anus, the liquid medicine is sprayed at the cloaca for 3 times continuously for 5 days, and the control group is treated for 5 days according to the conventional method. After 5 days, the semen quality of 2 groups of cocks was examined, respectively, and the results were as follows:
TABLE 3 influence of plant extract sprays on cock reproductive performance
From the above results, the amount of sperm, sperm motility, sperm density, effective sperm count and sperm aberration rate of the test group in example 4 were all better than those of the control group, and the beneficial effects of the present invention were obtained.
< example 5>
A plant extract spray for improving reproductive performance of cocks comprises the following medicinal materials in parts by weight: 45 parts of pine needle, 22 parts of perilla seed, 23 parts of paulownia flower, 12 parts of rhizoma polygonati, 13 parts of semen allii tuberosi, 3 parts of herba epimedii and 3 parts of eurycoma longifolia, and the selected medicinal materials are cleaned, dried and crushed into 100 meshes of medicinal powder. Mixing the pulverized medicinal powder with appropriate amount of boiling water, stirring to obtain paste, and freezing at-25 deg.C for 24 hr; freezing for 24 hr, taking out the pasty Chinese medicinal powder, and thawing at 25 deg.C. Mixing the pasty Chinese medicinal powder subjected to freeze thawing treatment with 95% ethanol solution at a mass ratio of 1:3, leaching at room temperature for 3h, vacuum filtering, repeating for 3 times (i.e. first ethanol extraction, second ethanol extraction, and third ethanol extraction), mixing filtrates, distilling under reduced pressure, volatilizing ethanol, adding 5 times volume of drinking water, stirring and concentrating on a vacuum rotary instrument with water bath constant temperature of 60 deg.C, concentrating to the same weight as the original medicinal material, and bottling in 100ml plastic spray bottle. In 27 months in 2017, in a chicken farm tested by the institute of animal science and technology of Guangxi university, 60 cocks with red and swollen bleeding symptoms, less semen and poor semen quality in cloacae after anus turning are randomly selected and isolated in a cock shed in the farm, and the cocks are randomly divided into 2 groups of 30 cocks. After the whole test group overturns the anus, the liquid medicine is sprayed at the cloaca for 3 times continuously for 5 days, and the control group is treated for 5 days according to the conventional method. After 5 days, the semen quality of 2 groups of cocks was examined, respectively, and the results were as follows:
TABLE 4 influence of plant extract sprays on cock reproductive performance
From the above results, it can be seen that the amount of sperm, sperm motility, sperm density, effective sperm count and sperm aberration rate of the test group in example 5 are all better than those of the control group, and the beneficial effects of the present invention are achieved.
< example 6>
A plant extract spray for improving reproductive performance of cocks comprises the following medicinal materials in parts by weight: 40 parts of pine needle, 20 parts of perilla seed, 20 parts of paulownia flower, 10 parts of rhizoma polygonati, 10 parts of semen allii tuberosi, 2 parts of herba epimedii and 2 parts of eurycoma longifolia, and the selected medicinal materials are cleaned, dried and crushed into 100 meshes of medicinal powder. Mixing the pulverized medicinal powder with appropriate amount of boiling water, stirring to obtain paste, and freezing at-25 deg.C for 24 hr; freezing for 24 hr, taking out the pasty Chinese medicinal powder, and thawing at 25 deg.C. Mixing the pasty Chinese medicinal powder subjected to freeze thawing treatment with 95% ethanol solution at a mass ratio of 1:3, leaching at room temperature for 3h, vacuum filtering, repeating for 3 times (i.e. first ethanol extraction, second ethanol extraction, and third ethanol extraction), mixing filtrates, distilling under reduced pressure, volatilizing ethanol, adding 5 times volume of drinking water, stirring and concentrating on a vacuum rotary instrument with water bath constant temperature of 60 deg.C, concentrating to the same weight as the original medicinal material, and bottling in 100ml plastic spray bottle. In a chicken breeding farm of Xinchengxian county-Hua-pasture Limited liability company of Nein, 13 days in 2017 and 3 and 17 days in 2017, 58 cocks with red and swollen bleeding symptoms, less sperm amount and poor sperm quality in cloacal cavities after anus turning are randomly selected and isolated in a cock shed, and the cocks are randomly divided into 2 groups, and 29 chickens are selected. After the whole test group overturns the anus, the liquid medicine is sprayed at the cloaca for 3 times continuously for 5 days, and the control group is treated for 5 days according to the conventional method. After 5 days, the semen quality of 2 groups of cocks was examined, respectively, and the results were as follows:
TABLE 5 Effect of plant extract sprays on cock reproductive Performance
From the above results, it can be seen that the amount of sperm, sperm motility, sperm density, effective sperm count and sperm aberration rate of the test group in example 6 are all better than those of the control group, and the beneficial effects of the present invention are achieved.
The plant extract spray for improving the reproductive performance of the cocks adopts a pure traditional Chinese medicine preparation, has mild medicine property and no irritation to the cocks, effectively treats the reproductive performance of the cocks in breeding, is administrated through a cloaca, is absorbed through intestinal mucosa, does not damage livers, relieves the stimulation of the medicines to gastrointestinal tracts, improves the blood concentration and the bioavailability of the cocks to the medicines, changes the traditional using method of the traditional Chinese medicines in the field of poultry production, extracts the traditional Chinese medicinal materials through freeze thawing and organic extraction technologies, obviously improves the extraction rate of effective components, has stable and lasting medicine effect, improves the reproductive performance of the cocks in a cloaca administration mode, and has the characteristics of environmental protection, small toxic and side effects, avoiding the damage to the livers and the intestines and stomach of the cocks and continuously and stably improving the reproductive performance of the cocks.
While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable to various fields of endeavor for which the invention may be embodied with additional modifications as would be readily apparent to those skilled in the art, and the invention is therefore not limited to the details given herein and to the embodiments shown and described without departing from the generic concept as defined by the claims and their equivalents.
Claims (2)
1. A plant extract spray for improving the reproductive performance of cocks is characterized by being prepared by the following steps: firstly, selecting pine needles, perilla seeds, paulownia flowers, sealwort, Chinese chives seeds, epimedium herb and eurycoma longifolia, then cleaning, drying, mixing and crushing, adding boiling water and stirring into pasty medicinal powder, freezing and thawing, carrying out primary alcohol extraction, secondary alcohol extraction, tertiary alcohol extraction, combining filtrates, removing ethanol, adding drinking water, stirring and concentrating, and finally filling to obtain the composition;
the plant medicinal materials comprise the following components in parts by weight: 40-50 parts of pine needle, 20-25 parts of perilla seed, 20-25 parts of paulownia flower, 10-15 parts of rhizoma polygonati, 10-15 parts of semen allii tuberosi, 2-6 parts of herba epimedii and 2-6 parts of eurycoma longifolia;
in all working links of the process preparation of the plant extract spray, except freezing and thawing and stirring concentration links, the environment of other working links is 18-30 ℃, the relative humidity is 30-70 percent, and the cleanliness grade of a production area is a common production area;
the crushing of the plant medicinal material components is required to be crushed into medicinal powder with the particle size of 100 meshes;
the freeze thawing process of the medicinal powder comprises the following steps: adding boiling water into the medicinal powder, stirring to obtain paste medicinal powder, freezing at-25 deg.C for 24 hr, and thawing at 25 deg.C;
the first alcohol extraction, the second alcohol extraction and the third alcohol extraction comprise the following specific steps: mixing the paste powder subjected to freeze thawing treatment with 95% ethanol solution at a mass ratio of 1:3, leaching at room temperature for 3h, filtering, performing repeated ethanol extraction for the second time and the third time, mixing the filtrates, and distilling under reduced pressure to remove ethanol;
the steps of removing ethanol and adding drinking water for stirring and concentrating are as follows: adding 5 times volume of drinking water into the ethanol extractive solution, stirring and concentrating in a vacuum rotary instrument with water bath constant temperature of 60 deg.C, concentrating until the weight of the medicinal components is the same as that of the original plant medicinal materials, and stopping concentration.
2. The plant extract spray for improving the reproductive performance of cocks as claimed in claim 1, wherein the plastic spray bottle with a volume of 100ml is selected for filling.
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Correction item: Patentee|Address|Patent agency|Agent Correct: Ge Hongwei|225700 Kaicheng Computer next to the new city bathing beach in Jiuqing Road, Xinghua City, Taizhou City, Jiangsu Province | Wuxi Fengchuang Intellectual Property Agency (Special General Partnership) 32461|Shan Hu False: Guangxi Hongzan Agricultural Development Group Co., Ltd.|537300 Li Kaili House, Yingbin Avenue, Shangdu Street, Pingnan County, Guigang City, Guangxi Zhuang Autonomous Region|Wuxi Fengchuang Intellectual Property Agency (Special General Partnership) 32461|Liu Yongfan Number: 51-02 Page: The title page Volume: 36 Correction item: Patentee|Address|Patent agency|Agent Correct: Ge Hongwei|225700 Kaicheng Computer next to the new city bathing beach in Jiuqing Road, Xinghua City, Taizhou City, Jiangsu Province | Wuxi Fengchuang Intellectual Property Agency (Special General Partnership) 32461|Shan Hu False: Guangxi Hongzan Agricultural Development Group Co., Ltd.|537300 Li Kaili House, Yingbin Avenue, Shangdu Street, Pingnan County, Guigang City, Guangxi Zhuang Autonomous Region|Wuxi Fengchuang Intellectual Property Agency (Special General Partnership) 32461|Liu Yongfan Number: 51-02 Volume: 36 |
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