CN107080770B

CN107080770B - Compound traditional Chinese medicine for preventing and treating fish ringworm and its preparation and use method

Info

Publication number: CN107080770B
Application number: CN201710525997.6A
Authority: CN
Inventors: 张立强; 罗杨志; 郑瑞珠; 张生元; 喻运珍; 艾桃山; 高祥林
Original assignee: Wuhan Choppor Fishery Bio Technology Co ltd; Wuhan Academy of Agricultural Sciences
Current assignee: Wuhan Choppor Fishery Bio Technology Co ltd; Wuhan Academy of Agricultural Sciences
Priority date: 2017-06-30
Filing date: 2017-06-30
Publication date: 2021-01-01
Anticipated expiration: 2037-06-30
Also published as: CN107080770A

Abstract

本发明公开了一种防治鱼类指环虫病的复合中药，主要的原料包括：椿根皮、路路通和南瓜子，所述椿根皮能够在鱼类指环虫病的防治前期有效杀灭指环虫及虫卵；所述路路通在鱼类指环虫病的防治前期和后期均对指环虫及虫卵有较显著的杀灭效果；所述南瓜子具有解毒杀虫的功效，主要作用于鱼类指环虫病的防治后期，用以杀灭少量残余的指环虫及虫卵；由椿根皮、路路通和南瓜子配伍而成的所述复合中药，可兼顾鱼类指环虫病的防治全程；本发明所述防治鱼类指环虫病的复合中药，不仅可以有效防治鱼类指环虫病，提高养殖存活率，解决指环虫对于单纯药物容易产生抗性的问题，还可在一定程度上抑制养殖水体中有害微生物的代谢生长，从而改善养殖水体的质量。The invention discloses a compound traditional Chinese medicine for preventing and treating fish ringworm disease. The main raw materials include: rhizome root bark, Lulutong and pumpkin seeds. The root bark can effectively kill fish ringworm disease in the early stage of prevention and treatment Ringworm and worm eggs; said Passepartout has significant killing effect on ringworm and worm eggs in the early and later stages of prevention and control of fish ringworm disease; the pumpkin seeds have the effect of detoxification and insecticidal, and the main effect is In the later stage of prevention and control of fish ringworm disease, it is used to kill a small amount of residual ringworm and eggs; the compound traditional Chinese medicine formed by the combination of root bark, Passepartout and pumpkin seeds can take into account fish ringworm disease The whole process of prevention and control; the composite traditional Chinese medicine for preventing and treating fish ringworm disease of the present invention can not only effectively prevent and control fish ringworm disease, improve the survival rate of culture, and solve the problem that ringworm is easy to produce resistance to simple drugs, but also can prevent and control fish ringworm disease. To a certain extent, it can inhibit the metabolic growth of harmful microorganisms in aquaculture water, thereby improving the quality of aquaculture water.

Description

Compound traditional Chinese medicine for preventing and treating fish dactylogyrus disease and preparation and use methods thereof

Technical Field

The invention relates to the field of disease prevention and control of freshwater fish culture, in particular to a compound traditional Chinese medicine for preventing and treating dactylogyrus disease of fish.

Background

Dactylogyrus disease is one of the most serious diseases in fish culture, and the parasitic dactylogyrus causes a great amount of death of cultured fishes by exciting clinical symptoms of fish 'gill rot' caused by infecting pathogenic bacteria and fungi. In view of the fact that dactylogyrus is easy to generate drug resistance, the control effect of the conventional national standard drugs on dactylogyrus is increasingly poor, the drug abuse phenomenon for controlling fish dactylogyrus is common, and the drug abuse phenomenon is one of the main inducements for causing the damage of aquaculture water environment and the quality safety of aquatic products, and therefore, the creation of a novel safe and effective drug for controlling fish dactylogus becomes an urgent affair. Chinese herbal medicine is a hot point for creating new medicines due to the characteristics of safety to animals, small side effect and mild environment. And the combined medication has more advantages in the face of the resistance problem of dactylogyrus to simple drugs.

Disclosure of Invention

An object of the present invention is to solve at least the above problems and to provide at least the advantages described later.

The invention also aims to provide the compound traditional Chinese medicine for preventing and treating the dactylogyrus disease of the fishes, which can effectively prevent and treat the dactylogyrus disease of the fishes, improve the culture survival rate, solve the problem that the dactylogyrus is easy to generate resistance to a simple medicament, and inhibit the metabolic growth of harmful microorganisms in culture water to a certain extent, thereby improving the quality of the culture water.

In order to achieve the purposes and other advantages, the invention provides a compound traditional Chinese medicine for preventing and treating fish dactylogyrosis, which mainly comprises the following raw materials in parts by weight:

10-30 parts of toona sinensis root bark, 10-30 parts of beautiful sweetgum fruit and 10-70 parts of pumpkin seed.

Preferably, the compound traditional Chinese medicine for preventing and treating fish dactylogyrus disease further comprises the following auxiliary materials in parts by weight: 1-5 parts of garlic powder, 1-3 parts of medlar, 1-3 parts of eucommia bark, 1-7 parts of verbena, 1-3 parts of scutellaria baicalensis, 1-7 parts of dried ginger, 1-3 parts of radix stemonae, 1-7 parts of radix sophorae flavescentis, 1-3 parts of pomegranate bark, 1-3 parts of rhizoma paridis and 1-5 parts of green bristlegrass.

Preferably, the compound traditional Chinese medicine for preventing and treating fish dactylogyrus disease comprises the following raw materials in parts by weight: 20 parts of ailanthus bark, 20 parts of beautiful sweetgum fruit, 40 parts of pumpkin seed, 3 parts of garlic powder, 2 parts of medlar, 2 parts of eucommia bark, 4 parts of verbena, 2 parts of scutellaria baicalensis, 4 parts of dried ginger, 2 parts of radix stemonae, 4 parts of radix sophorae flavescentis, 2 parts of pomegranate bark, 2 parts of rhizoma paridis and 3 parts of green bristlegrass.

The invention also provides a preparation method of the compound traditional Chinese medicine for preventing and treating dactylogyrniosis of fishes, which comprises the following steps:

step one, crushing the toona sinensis root bark, the beautiful sweetgum fruit, the pumpkin seed, the garlic powder, the medlar, the eucommia bark, the verbena, the baical skullcap root, the dried ginger, the sessile stemona root, the lightyellow sophora root, the pomegranate bark, the paris polyphylla and the green bristlegrass to more than 30 meshes for later use respectively.

And step two, sieving the raw materials crushed in the step one by a sieve with more than 30 meshes respectively, and uniformly mixing to prepare a solid powder for later use.

And step three, dissolving the solid powder in water, performing water extraction by gradient temperature rise within the range of 50-100 ℃, standing overnight, and filtering out dregs of a decoction to obtain the compound traditional Chinese medicine extracting solution for preventing and treating the dactylogyrosis of the fishes.

Preferably, in the first step, the toona sinensis root bark, the fructus liquidambaris, the pumpkin seeds, the garlic powder, the medlar, the eucommia ulmoides, the verbena officinalis, the scutellaria baicalensis, the rhizoma zingiberis, the radix stemonae, the radix sophorae flavescentis, the pomegranate bark, the rhizoma paridis and the green bristlegrass are fully dried before being crushed.

Preferably, in the second step, the crushed raw materials are respectively sieved by a 40-mesh sieve and uniformly mixed to prepare the solid powder.

Preferably, in the third step, the temperature gradient is as follows: keeping the temperature at 60 ℃ for 2h, keeping the temperature at 70 ℃ for 2h, keeping the temperature at 80 ℃ for 2h, and keeping the overnight time at the lowest 6 h.

The invention also provides a use method of the compound traditional Chinese medicine for preventing and treating fish dactylogyrus disease, namely the compound traditional Chinese medicine is put into a culture water body for use, and the putting amount is based on the standard that the final concentration of the raw materials is 40-100 mg/L.

The invention at least comprises the following beneficial effects:

the invention adopts the toona sinensis root bark, the fructus liquidambaris and the pumpkin seeds as main active ingredients for killing dactylogyrus, the toona sinensis root bark can effectively kill dactylogyrus and worm eggs in the early stage of prevention and treatment of dactylogyrus of fishes, and the insecticidal rate is more than 92%; the fructus liquidambaris has a remarkable killing effect on dactylogyrus and ova in both the early stage and the later stage of prevention and treatment of dactylogyrus diseases of fishes; the pumpkin seeds have the effects of detoxifying and killing insects, the insecticidal rate is more than 92%, and the pumpkin seeds are mainly used for killing a small amount of residual dactylogyrus and ova in the later stage of prevention and treatment of dactylogyrus diseases of fishes; the compound traditional Chinese medicine prepared from the toona sinensis root bark, the fructus liquidambaris and the pumpkin seeds can kill dactylogyrus imagoes and ova in the whole process of preventing and treating the dactylogyrus disease of the fishes, and the insecticidal rate is as high as 97%; the compound traditional Chinese medicine can effectively prevent and treat dactylogyrus disease of fishes, improve the culture survival rate, solve the problem that the dactylogyrus is easy to generate resistance to simple medicines, and can inhibit the metabolic growth of harmful microorganisms in culture water to a certain extent, thereby improving the quality of the culture water.

Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.

Drawings

FIG. 1 is a statistical chart of the killing effect of the compound traditional Chinese medicine on dactylogyrus on the third day in the first experiment of the invention;

FIG. 2 is a statistical chart of the killing effect of the compound traditional Chinese medicine on dactylogyrus on the eighth day in the first experiment of the invention;

FIG. 3 is a statistical chart of the killing effect of the compound traditional Chinese medicine on dactylogyrus on the third day in the second experiment of the invention;

FIG. 4 is a statistical chart of the killing effect of the compound traditional Chinese medicine on dactylogyrus on the eighth day in experiment two of the present invention.

Detailed Description

The present invention is described in further detail below to enable those skilled in the art to practice the invention with reference to the description.

It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.

The invention provides a compound traditional Chinese medicine for preventing and treating dactylogyrus disease of fishes, which comprises the following raw materials in parts by weight: 10-30 parts of toona sinensis root bark, 10-30 parts of beautiful sweetgum fruit and 10-70 parts of pumpkin seed.

In the scheme, the toona sinensis root bark, the fructus liquidambaris and the pumpkin seeds are used as main active ingredients for killing dactylogyrus, as shown in figures 1 and 2, the toona sinensis root bark can effectively kill dactylogus and worm eggs in the early stage of prevention and treatment of dactylogyrus diseases of fishes, and the insecticidal effect is more than 92%; the fructus liquidambaris has a remarkable killing effect on dactylogyrus and ova in both the early stage and the later stage of prevention and treatment of dactylogyrus diseases of fishes; the pumpkin seeds have the efficacy of detoxifying and killing insects, the insecticidal effect is more than 92%, and the pumpkin seeds are mainly used for killing a small amount of residual dactylogyrus and ova in the later stage of prevention and treatment of dactylogyrus diseases of fishes; the compound traditional Chinese medicine prepared from the toona sinensis root bark, the fructus liquidambaris and the pumpkin seeds can kill dactylogyrus imagoes and ova in the whole process of preventing and treating the dactylogyrus disease of the fishes, and the insecticidal rate can reach 97 percent at most; not only can effectively prevent and treat dactylogyrus disease of fishes, improve the culture survival rate, solve the problem that the dactylogyrus is easy to generate resistance to simple drugs, but also can inhibit the metabolic growth of harmful microorganisms in the culture water body to a certain extent, thereby improving the quality of the culture water body.

In a preferred scheme, the compound traditional Chinese medicine for preventing and treating fish dactylogyrus disease further comprises the following auxiliary materials in parts by weight: 1-5 parts of garlic powder, 1-3 parts of medlar, 1-3 parts of eucommia bark, 1-7 parts of verbena, 1-3 parts of scutellaria baicalensis, 1-7 parts of dried ginger, 1-3 parts of radix stemonae, 1-7 parts of radix sophorae flavescentis, 1-3 parts of pomegranate bark, 1-3 parts of rhizoma paridis and 1-5 parts of green bristlegrass.

In the scheme, the compatibility of the garlic powder, the eucommia bark and the scutellaria baicalensis has broad-spectrum antibacterial, bacteriostatic and bactericidal effects, the growth of microorganisms in a culture water body can be inhibited to a certain degree, secondary infection is not easy to occur, the survival rate is effectively improved, the compatibility of the verbena, the rhizoma zingiberis, the radix stemonae, the radix sophorae flavescentis and the rhizoma paridis can improve the insecticidal effect, the compatibility of the pomegranate rind and the green bristlegrass can effectively treat gill rot of fishes caused by finger ring worms due to infection, the medlar can slow down the accumulation speed of organic matters in the culture water body, the water quality is effectively improved, and as shown in fig. 3 or fig. 4, the composite traditional Chinese medicine added with the auxiliary materials such as the garlic powder.

In a preferred scheme, the compound traditional Chinese medicine for preventing and treating fish dactylogyrosis comprises the following raw materials in parts by weight: 20 parts of ailanthus bark, 20 parts of beautiful sweetgum fruit, 40 parts of pumpkin seed, 3 parts of garlic powder, 2 parts of medlar, 2 parts of eucommia bark, 4 parts of verbena, 2 parts of scutellaria baicalensis, 4 parts of dried ginger, 2 parts of radix stemonae, 4 parts of radix sophorae flavescentis, 2 parts of pomegranate bark, 2 parts of rhizoma paridis and 3 parts of green bristlegrass.

The invention also provides a preparation method of the compound traditional Chinese medicine for preventing and treating dactylogyrus disease of fishes, wherein the preparation method comprises the following steps:

In a preferred scheme, the preparation method of the compound traditional Chinese medicine for preventing and treating fish dactylogyrus disease comprises the step one of fully drying the toona sinensis root bark, the fructus liquidambaris, the pumpkin seeds, the garlic powder, the medlar, the eucommia ulmoides, the verbena, the scutellaria baicalensis, the rhizoma zingiberis, the radix stemonae, the radix sophorae flavescentis, the pomegranate rind, the rhizoma paridis and the green bristlegrass before crushing.

In the scheme, the dried medicinal material has extremely low moisture content, and is easier to crush uniformly into small particles which pass through a sieve with more than 30 meshes.

In a preferable scheme, in the preparation method of the compound traditional Chinese medicine for preventing and treating the fish dactylogyrus disease, the crushed raw materials in the step two are respectively sieved by a 40-mesh sieve and are uniformly mixed to prepare the solid powder.

In the above scheme, the solid powder is convenient to store and use.

In a preferred scheme, the preparation method of the compound traditional Chinese medicine for preventing and treating fish dactylogyrus disease comprises the following steps: keeping the temperature at 60 ℃ for 2h, keeping the temperature at 70 ℃ for 2h, keeping the temperature at 80 ℃ for 2h, and keeping the overnight time at the lowest 6 h.

In the scheme, the water extraction is carried out from low to high by setting the temperature gradient, so that the effective components of the medicine can be fully extracted on the premise of not damaging various medicine components.

Example 1

A compound traditional Chinese medicine for preventing and treating dactylogyrosis of fishes mainly comprises the following raw materials in parts by weight: 10 parts of toona sinensis root bark, 10 parts of beautiful sweetgum fruit and 10 parts of pumpkin seeds.

Step one, respectively crushing the toona sinensis root bark, the beautiful sweetgum fruit and the pumpkin seeds to 30 meshes for later use.

And step two, respectively sieving the raw materials crushed in the step one by a 30-mesh sieve, and uniformly mixing to prepare a solid powder for later use.

And step three, dissolving the solid powder in water, performing water extraction at a constant temperature of 50 ℃ for 2h, at a constant temperature of 60 ℃ for 2h and at a constant temperature of 70 ℃ for 2h according to a temperature gradient, standing overnight, and filtering out dregs to obtain the compound traditional Chinese medicine extracting solution for preventing and treating fish dactylogyrosis.

Example 2

A compound traditional Chinese medicine for preventing and treating dactylogyrosis of fishes mainly comprises the following raw materials in parts by weight: the Chinese toon root bark, the beautiful sweetgum fruit and the pumpkin seed 20.

Step one, respectively crushing the toona sinensis root bark, the beautiful sweetgum fruit and the pumpkin seeds to 40 meshes for later use.

And step two, respectively sieving the raw materials crushed in the step one by a 40-mesh sieve, and uniformly mixing to prepare a solid powder for later use.

And step three, dissolving the solid powder in water, performing water extraction at a constant temperature of 60 ℃ for 2h, at a constant temperature of 70 ℃ for 2h and at a constant temperature of 80 ℃ for 2h according to a temperature gradient, standing overnight, and filtering out dregs to obtain the compound traditional Chinese medicine extracting solution for preventing and treating fish dactylogyrosis.

Example 3

A compound traditional Chinese medicine for preventing and treating dactylogyrosis of fishes mainly comprises the following raw materials in parts by weight: 30 parts of toona root bark, 30 parts of beautiful sweetgum fruit and 70 parts of pumpkin seed.

Step one, respectively crushing the toona sinensis root bark, the beautiful sweetgum fruit and the pumpkin seeds to 50 meshes for later use.

And step two, respectively sieving the raw materials crushed in the step one by a 50-mesh sieve, and uniformly mixing to prepare a solid powder for later use.

Dissolving the solid powder in water, performing water extraction at a constant temperature of 70 ℃ for 2h, at a constant temperature of 80 ℃ for 2h and at a constant temperature of 90 ℃ for 2h according to a temperature gradient, standing overnight, and filtering out dregs to obtain the compound traditional Chinese medicine extracting solution for preventing and treating fish dactylogyrosis.

Example 4

A compound traditional Chinese medicine for preventing and treating dactylogyrosis of fishes mainly comprises the following raw materials in parts by weight: the Chinese toon root bark tea comprises 20 parts of Chinese toon root bark, 20 parts of beautiful sweetgum fruit, 40 parts of pumpkin seed, 3 parts of garlic powder, 1 part of medlar, 1 part of eucommia bark, 4 parts of verbena, 1 part of baical skullcap root, 4 parts of dried ginger, 1 part of sessile stemona root, 4 parts of lightyellow sophora root, 1 part of pomegranate bark, 1 part of Chinese paris rhizome and 3 parts of green bri.

Step one, crushing the toona sinensis root bark, the beautiful sweetgum fruit, the pumpkin seed, the garlic powder, the medlar, the eucommia bark, the verbena, the baical skullcap root, the dried ginger, the sessile stemona root, the lightyellow sophora root, the pomegranate bark, the paris polyphylla and the green bristlegrass into 40 meshes for later use respectively.

The effectiveness of the present invention is further illustrated by the following experiments.

Experiment one

In order to observe the effect of the single component of the compound traditional Chinese medicine on dactylogyrus adults and ova, 2.5g of the toona sinensis root bark, the beautiful sweetgum fruit and the pumpkin seed solid powder are respectively weighed, are respectively dissolved in 200mL of water, are subjected to water extraction overnight at the constant temperature of 60 ℃ for 2h, the constant temperature of 70 ℃ for 2h and the constant temperature of 80 ℃ for 2h according to the temperature gradient, and are concentrated to 50mL of a medicine stock solution after filter residues are removed.

In order to observe the effect of the composite traditional Chinese medicine composition on dactylogyrus adults and ova, 0.625g of toona sinensis root bark, 0.625g of beautiful sweetgum fruit and 1.25g of pumpkin seeds are respectively weighed and sieved by a 40-mesh sieve to be mixed to obtain the solid powder, the solid powder is dissolved in 200mL of water, the water is extracted overnight at the constant temperature of 60 ℃ for 2h, at the constant temperature of 70 ℃ for 2h and at the constant temperature of 80 ℃ for 2h, and the mixture is concentrated to 50mL of composite traditional Chinese medicine stock solution after filter residues are removed.

Adding 50L of water into the aquarium, aerating for 8h, temporarily culturing 10 goldfishes in each group for 24h, and respectively adding the drug stock solution and the compound traditional Chinese medicine stock solution (50 mL of water is added into a blank control group), wherein the final concentration of the drug is 50mg/L, and the experimental temperature is 25 ℃. As the generation cycle of the dactylogyrus is about 8 days, the experimental sampling time is 3 days and 8 days respectively.

TABLE 1 amounts of dactylogyrus on days three and eight after the action of single and combination drugs

As can be seen from the above experiments, as shown in Table 1 and FIG. 1, on the third day of drug action, the number of polypide on the fish in the toona sinensis root-bark group is greatly reduced compared with the control group, and the insecticidal rate is about 92% (P is less than 0.01, the P value is the probability of making mistakes which is considered to be effective in statistics, namely the probability of making mistakes is overall representative, P is more than or equal to 0.05 and more than or equal to 0.01 and has statistical significance, P is more than or equal to 0.01 and more than or equal to 0.001 and has high statistical significance, otherwise, the larger the P value is, the larger the influence degree of variables in the statistical process on the result is), so that the difference is; compared with a control group, the liquidambar formosana hance group has the advantages that the number of insect bodies is greatly reduced, the insecticidal rate is about 80% (P is less than 0.05), and the remarkable difference exists; compared with a control group, the number of the polypide on the fish is not significantly different (P is more than 0.05); compared with a control group, the number of the polypide on the fish is greatly reduced, the insecticidal rate is about 87 percent (P is less than 0.01), and the difference is very obvious.

As shown in table 1 and fig. 2, on the eighth day of drug action, there was no significant difference in the number of worm bodies on the fish (P > 0.05) in the toona sinensis root bark group compared to the control group; compared with a control group, the liquidambar formosana hance group has the advantages that the number of polypides is reduced little, the insecticidal rate is about 62% (P is less than 0.05), and the remarkable difference exists; compared with a control group, the pumpkin group has the advantages that the number of the insects on the fish is greatly reduced, the insecticidal rate is about 92% (P is less than 0.01), and the differences are extremely obvious; compared with a control group, the number of the polypide on the fish is reduced to the minimum, the insecticidal rate is about 97 percent (P is less than 0.01), and the difference is very obvious; the experimental data show that the compound traditional Chinese medicine has higher killing effect efficiency on dactylogyrus imagoes and ovum compared with single component.

Experiment two

The experiment is provided with two experimental groups, wherein the experimental group A is the effect of the composite traditional Chinese medicine composition on dactylogyrus adults and ova, and the experimental group B is the effect of the composite traditional Chinese medicine composition after auxiliary materials are added into the composite traditional Chinese medicine on the dactylogus adults and the ova.

Experimental group a: weighing 1.25g of toona sinensis root bark, 1.25g of beautiful sweetgum fruit and 2.5g of pumpkin seeds respectively, sieving with a 40-mesh sieve, mixing to obtain the solid powder, dissolving the solid powder in 400mL of water, performing water extraction at a constant temperature of 60 ℃ for 2h, at a constant temperature of 70 ℃ for 2h and at a constant temperature of 80 ℃ for 2h overnight, removing filter residues, and concentrating to 100mL of a composite traditional Chinese medicine A stock solution.

Experimental group B: weighing 1.25g of ailanthus bark, 1.25g of beautiful sweetgum fruit, 2.5g of pumpkin seed, 0.188g of garlic powder, 0.125g of medlar, 0.125g of eucommia bark, 0.25g of verbena, 0.125g of scutellaria baicalensis, 0.25g of dried ginger, 0.125g of radix stemonae, 0.25g of radix sophorae flavescentis, 0.125g of pomegranate bark, 0.125g of paris polyphylla and 0.188g of green bristlegrass respectively, crushing, sieving by a 40-mesh sieve, mixing to obtain the solid powder, dissolving the solid powder in 400mL of water, performing water extraction at constant temperature of 60 ℃ for 2h, at constant temperature of 70 ℃ for 2h and at constant temperature of 80 ℃ for 2h overnight, removing filter residues, and concentrating to 100mL of composite traditional Chinese medicine B raw liquid.

Adding 100L of water into aquarium, aerating for 8 hr, temporarily culturing 10 goldfishes in each group for 24 hr, adding compound Chinese medicinal material A stock solution and compound Chinese medicinal material B stock solution (adding 100mL of water into blank control group), and testing at 25 deg.C.

TABLE 2 the number of dactylogyrus on the third and eighth days after the action of the combination drug and the combination drug with the addition of the adjuvant

According to the experiment, the raw materials of the compound traditional Chinese medicine A are the Chinese toon root bark, the beautiful sweetgum fruit and the pumpkin seed which are compounded according to the weight ratio of 1:1:2, as shown in the table 2 and the figure 3, on the third day of the drug action, compared with a blank control group, the compound traditional Chinese medicine A group has the advantages that the number of the polypide on the fish is greatly reduced, the insecticidal rate is about 86% (P is less than 0.01), and the extremely obvious difference exists; the compound traditional Chinese medicine B is formed by adding auxiliary materials such as garlic powder, medlar, eucommia bark and the like into the compound traditional Chinese medicine A, as shown in the table 2 and the figure 3, on the third day of the drug action, compared with a control group, the compound traditional Chinese medicine B group has the advantages that the number of fish worms on the fish is greatly reduced, the insecticidal rate is about 91% (P is less than 0.01), and the difference is very obvious.

As shown in table 2 and fig. 4, on the eighth day of drug action, compared with the blank control group, the number of the polypide on the fish in the compound traditional Chinese medicine group a is greatly reduced, the insecticidal rate is about 95% (P is less than 0.01), and the difference is very significant; as shown in Table 2 and FIG. 4, on the eighth day of drug action, no worm bodies could be detected on the fish in the compound traditional Chinese medicine group B compared with the control group (P is less than 0.01), and there is a very significant difference.

The invention solves the problems that the traditional national standard medicine has increasingly poor prevention and treatment effect on dactylogyrus, abuse of medicine and harm to the safety and quality of aquatic products, reduces the morbidity and morbidity of the dactylogyrus and improves the culture survival rate. The compound traditional Chinese medicine solves the problem that the dactylogyrus is easy to generate drug resistance to a single medicine, and the compound traditional Chinese medicine can keep the killing effect of the dactylogyrus in both the early and later periods.

While the embodiments of the invention have been disclosed above, it is not limited to the applications listed in the description and the embodiments, but it can be fully applied to various fields suitable for the invention, and further modifications can be easily implemented by those skilled in the art. It is therefore intended that the invention not be limited to the exact details and illustrations described and illustrated herein, but fall within the scope of the appended claims and equivalents thereof.

Claims

1. A compound traditional Chinese medicine for preventing and treating fish ringworm disease, which is made from the following raw materials by weight: 10-30 parts of Chinese root bark, 10-30 parts of Passepartout, 10-70 parts of pumpkin seeds, 1- 5 parts, 1-3 parts of wolfberry, 1-3 parts of Eucommia ulmoides, 1-7 parts of verbena, 1-3 parts of skullcap, 1-7 parts of dried ginger, 1-3 parts of 100 parts, 1-7 parts of Sophora flavescens, pomegranate 1-3 parts of the skin, 1-3 parts of the heavy building and 1-5 parts of the foxtail.

2. the composite traditional Chinese medicine of preventing and treating fish ringworm disease as claimed in claim 1, is made up of the raw material of following weight portion: 20 parts of rhizoma root bark, 20 parts of Passepartout, 40 parts of pumpkin seeds, 3 parts of garlic powder, 2 parts of wolfberry, 2 parts of Eucommia, 4 parts of Verbena, 2 parts of Scutellaria baicalensis, 4 parts of dried ginger, 2 parts of Bai Bu, 4 parts of Sophora flavescens, 2 parts of pomegranate peel, 2 parts of Chonglou and 3 parts of Setaria.

3. a preparation method of the compound traditional Chinese medicine of preventing and treating fish ringworm disease as claimed in claim 1, wherein, comprises the following steps:

Step 1. Pulverize the root bark, Passepartout, pumpkin seeds, garlic powder, wolfberry, Eucommia, Verbena, Scutellaria baicalensis, dried ginger, Bai Bu, Sophora flavescens, pomegranate peel, Chonglou and foxtail to 30 mesh or more for later use ;

Step 2, the various raw materials pulverized in step 1 are respectively passed through a sieve of more than 30 meshes and mixed uniformly to make a solid powder for subsequent use;

Step 3: Dissolving the solid powder in water, carrying out water extraction according to a gradient temperature in the range of 50-100 ° C, overnight and filtering out the medicinal residues, to obtain the extract of the compound traditional Chinese medicine for the prevention and treatment of fish ringworm disease .

4. the preparation method of the compound traditional Chinese medicine of preventing and treating fish ringworm disease as claimed in claim 3, wherein, in described step 1, described toon root bark, Passepartout, pumpkin seeds, garlic powder, wolfberry, Eucommia, Verbena, Scutellaria, Ginger, Bulbul, Sophora Radix, Pomegranate Bark, Chonglou and Setaria are fully dried before crushing.

5. The preparation method of the compound traditional Chinese medicine for preventing and treating fish ringworm disease as claimed in claim 3, wherein, in the step 2, the pulverized various raw materials are respectively mixed with a 40 mesh sieve to make a solid powder.

6. The preparation method of the compound traditional Chinese medicine for preventing and treating fish ringworm disease as claimed in claim 3, wherein, in the step 3, the temperature gradient is: 60°C constant temperature for 2h, 70°C constant temperature for 2h, and 80°C constant temperature for 2h , the overnight time is a minimum of 6h.