CN103564495B - Propolis and cordycepic hypha powder composition, as well as preparation and application thereof - Google Patents

Abstract

The invention provides a health food composition of propolis and Cordyceps mycelia, which is composed of 45%-60% of propolis, 30-45% of Cordyceps mycelium powder and 7-10% of maltodextrin. Ultrasonic extraction with purified water respectively, the extracted filtrates are combined, the raw material Cordyceps mycelia powder is infiltrated with water in proportion, and stirred evenly; with the help of a magnetic stirrer, the prepared propolis extract is evenly added to the prepared Cordyceps sinensis In the mycelia aqueous solution, the mixed liquid is filtered, dried under reduced pressure or freeze-dried and other methods. The composition of the present invention can significantly enhance the cellular immunity and humoral immunity of the body, and the intercombination of propolis and cordyceps has synergistic effects; in vivo anti-aging experiments show that the composition has obvious anti-fatigue, anti-oxidation, memory-improving and anti-aging effects. The contents of total flavonoids and polysaccharides, the main active ingredients of the extract of the composition of the present invention, are respectively limited, so as to facilitate the quality control of the product.

Description

Composition of propolis and cordyceps mycelium powder and its preparation and application

technical field

The invention belongs to the field of health food, and relates to nutrition and health food, in particular to a propolis and cordyceps mycelium health food composition prepared from propolis and cordyceps mycelium powder, a preparation method and application thereof.

Background technique

Propolis is a fragrant substance that bees collect plant resin and mix it with beeswax and other secretions. More than 20 types and more than 500 kinds of natural ingredients have been found in propolis, including more than 30 kinds of mineral elements necessary for human body, 20 kinds of amino acids, dozens of aromatic compounds, and more than 100 kinds of flavonoids. There are also rich organic acids, terpenes, vitamins, lignans and other biologically active natural ingredients. Propolis tastes bitter, pungent, and cold, and returns to the spleen and stomach meridian. It has the functions of nourishing weakness, transforming turbid fat, quenching thirst, detoxifying and detumescence, astringent and granulation, and used for physical deficiency, early deficiency, hyperlipidemia, diabetes, etc. , and has a wide range of biological effects such as antibacterial and anti-inflammatory, anti-virus, anti-fungal, toxin elimination, immune enhancement, blood vessel softening, blood purification, microcirculation improvement, anti-oxidation, anti-tumor, cytotoxin, local anesthesia, and promotion of tissue regeneration.

The Cordyceps mycelium is a finished product made by using the Cordyceps fungus isolated from fresh Cordyceps sinensis in Qinghai - Paecilomyces spp. through submerged fermentation culture, and the fermentation product is filtered and dried. Modern medical research shows that Cordyceps powder contains about 7% of cordycepic acid, 28.9% of carbohydrates, about 8.4% of fat, about 25% of protein, and 82.2% of fat is unsaturated fatty acid. In addition, it also contains vitamin B12, ergosterol, Six-carbon sugar alcohols, alkaloids, vitamin B12, fat, protein, etc. Among them, amino acids, polysaccharides, and cordycepic acid are the main functional components. Polysaccharides are the most important and the most biologically active compounds in Cordyceps sinensis. They are considered to be the medicinal ingredients of Cordyceps sinensis with two-way immune regulation, and have anti-tumor, anti-inflammatory, hypoglycemic, and lipid-lowering effects. Cordyceps is sweet, mild and non-toxic. It is a well-known nourishing and strengthening medicine. It has the effects of nourishing lungs and kidneys, relieving cough and reducing phlegm, dilating trachea, calming, anti-bacterial, and lowering blood pressure. It also has anti-tumor, anti-oxidation, anti-inflammatory and Improve the body's immunity and other functions, can decompose the fatigue substances in the muscles, and improve exercise ability.

At present, propolis and Cordyceps mycelium powder have each developed health food with various functions. This patent uses different proportions of ethanol and pure water to maximize the extraction of active ingredients in propolis and Cordyceps fungus, and simultaneously extracts propolis and Cordyceps fungus powder After the liquid is compounded, the synergistic effect of flavonoids and phenolic acids in propolis and polysaccharides in Cordyceps mycelium is fully utilized. It has multiple functions such as assisting in lowering blood sugar, and the compound composition prepared by this patent has fine particles and is easily soluble in water, so that the active ingredients in propolis and Cordyceps mycelium are more easily absorbed by the human body.

Contents of the invention

The purpose of the present invention is to provide a propolis and Cordyceps mycelium health food composition, the composition of the present invention is made up of the following raw materials in percentage by weight: propolis 45%-60%; Cordyceps mycelium powder 30-45%, malt paste Refined 7-10%, the sum of the percentages of each component should be equal to 100%.

Another object of the present invention is to provide the preparation method of the composition, which is achieved through the following technical solutions: (1) select the propolis raw material according to the above weight percentage, and use 95%, 75% and 50% water Ethanol and purified water were used to ultrasonically extract propolis according to the solid-liquid ratio of 1:4, and the ultrasonic extraction time was controlled at 30-45 minutes, and the extracted filtrates were combined to obtain propolis extract; Purified water is ultrasonically extracted twice according to the material-to-liquid ratio of 1:10. The ultrasonic extraction time is controlled at 30-45 minutes, filtered, and the filter residue is ultrasonically soaked with 50% and 75% ethanol aqueous solutions according to the material-to-liquid ratio of 1:4 and 1:4 respectively. Extract once, combine the extracted filtrates, dry under reduced pressure to remove ethanol, obtain the water extract of Cordyceps mycelium, then add maltodextrin with a mass ratio of 7-10%, and mix well to obtain an aqueous solution of Cordyceps mycelium maltodextrin; (3) With the help of a magnetic stirrer, evenly add the prepared propolis extract into the prepared Cordyceps mycelia maltodextrin aqueous solution, mix thoroughly for 30 minutes, and control the temperature at 40 degrees; (4) Mix the mixture The dried mycelia of propolis and Cordyceps can be obtained by filtering, drying under reduced pressure or freeze-drying. The total flavonoid content in the prepared propolis cordyceps mycelium dried product is not less than 4.0%, the polysaccharide is not less than 6%, and the adenosine is not less than 0.01%.

Another object of the present invention is to provide the application of the composition in the preparation of propolis and Cordyceps mycelia health products. The health product is prepared by adding the dried product of propolis and Cordyceps mycelium, adding appropriate amount of auxiliary materials or solvents according to the existing conventional method. The dosage form of the health product is tablet, capsule, granule, sustained-release tablet, drop or oral liquid wait.

Aiming at the incomplete water-solubility of propolis and incomplete extraction of nutrient components, different proportions of ethanol water extraction are used, and water-soluble components such as polysaccharides in Cordyceps mycelium are fully utilized to prepare yin and yang, nourishing qi and blood, anti-oxidation, and strengthening the human body. A propolis and cordyceps mycelium health food composition with multiple functions such as immunity and strong water solubility.

Compared with the prior art, the present invention has the following beneficial effects: using propolis and cordyceps mycelium powder as main raw materials, the propolis and cordyceps mycelium powder are fully preserved through special processing technology, and the obtained product has good water solubility and is easy to be absorbed by human body. The product can be made into a variety of dosage forms; it not only complements the respective nutritional effects of propolis and Cordyceps mycelium, but also synergizes the shared effects. It has multiple functions such as nourishing yin and tonifying yang, anti-fatigue, anti-oxidation, anti-aging, and auxiliary hypoglycemia , long-term use has no toxic side effects, can prevent diseases, improve malnutrition and fatigue, and has the health care and medical effects of delaying aging. Composition of the present invention is carried out pharmacological research, and the result shows: composition of the present invention can obviously enhance the cellular immunity of body, humoral immunity function, and propolis and Chinese caterpillar fungus have synergistic effect mutually; Anti-fatigue, anti-oxidant, improve memory and anti-aging effect. The contents of total flavonoids and polysaccharides, the main active ingredients of the extract of the composition of the present invention, are respectively limited, so as to facilitate the quality control of the product.

Detailed ways

The present invention will be further described below in conjunction with embodiment. The propolis and cordyceps mycelium health food composition of the present invention is made of the following raw materials in percentage by weight: 45%-60% of propolis; 30-45% of cordyceps mycelium powder and 7-10% of maltodextrin. The sum of the percentages of each component should be equal to 100%.

Example 1

a) Select 550 grams of natural fresh propolis, freeze and crush it into fine powder, mix it with pure drinking water and edible alcohol to form solutions with alcohol content of 95%, 75% and 50% respectively, and extract propolis by ultrasonic wave with pure water. The ratio of propolis to extraction solutions with different concentrations of alcohol content is 1:4, and the ultrasonic extraction time is controlled at 40 minutes. After solid-liquid separation, the filtrates are combined to obtain propolis extract.

b) Select 350 grams of Cordyceps mycelium powder, add pure water according to the material-to-liquid ratio of 1:10, control the ultrasonic extraction time for 30 minutes, filter, and use 50% and 75% ethanol water with 50% and 75% ethanol water to ultrasonically respectively according to the volume ratio of 1:4 Leach each once, combine the filtrates, and dry under reduced pressure to remove ethanol to obtain an aqueous extract of Cordyceps mycelium, then add 100 g of maltodextrin, and mix well to obtain an aqueous solution of Cordyceps mycelium maltodextrin.

c) Add the propolis extract prepared in (a) evenly into the prepared Cordyceps mycelia maltodextrin aqueous solution under the action of magnetic stirring, mix well for 30 minutes, and control the temperature at 40 degrees.

d) filtering, concentrating and drying the propolis and cordyceps hyphae aqueous solution after the above reaction to obtain the product.

e) Prepare propolis and Cordyceps according to the above method, pulverize and mix evenly, add auxiliary materials and mix evenly, granulate according to the conventional method, dry, compress into tablets, and coat.

Example 2

a) Select 500 grams of natural fresh propolis, freeze and crush it into fine powder, mix it with pure drinking water and edible alcohol to form solutions with alcohol content of 95%, 75% and 50% respectively, and extract propolis by ultrasonic wave with pure water. The ratio of propolis to extraction solutions with different concentrations of alcohol content is 1:4, and the ultrasonic extraction time is controlled at 40 minutes. After solid-liquid separation, the filtrates are combined to obtain propolis extract.

b) Select 400 grams of Cordyceps mycelium powder, add pure water according to the material-to-liquid ratio of 1:10, control the ultrasonic extraction time for 45 minutes, filter, and filter the filter residues with 50% and 75% ethanol water according to the ratio of 1:4. Extract each once, combine the filtrates, and dry under reduced pressure to remove ethanol to obtain an aqueous extract of Cordyceps mycelium, then add 100 g of maltodextrin, and mix well to obtain an aqueous solution of Cordyceps mycelium maltodextrin.

c) Add the propolis extract prepared in (a) evenly into the prepared aqueous solution of Cordyceps mycelia under the action of magnetic stirring, mix thoroughly for 30 minutes, and control the temperature at 40 degrees.

d) filtering, concentrating and drying the propolis and cordyceps hyphae aqueous solution after the above reaction to obtain the product.

e) Prepare propolis and Cordyceps according to the above method, pulverize and mix them evenly, dissolve the dry propolis and Cordyceps mycelia powder with water at a ratio of 1:3, add appropriate amount of auxiliary materials, and prepare oral liquid according to conventional methods.

The impact of embodiment 3 on immune function

Preparation of the test drug: select 600 grams of natural fresh propolis, freeze and crush it into fine powder, mix it with drinking purified water and edible alcohol to form solutions with alcohol content of 95%, 75%, and 50% concentration, and ultrasonically extract it with pure water Propolis. The ratio of propolis to extraction solutions with different concentrations of alcohol content is 1:4. Ultrasonic extraction is performed for 40 minutes. After solid-liquid separation, the filtrates are combined to obtain propolis extract.

Select 330 grams of Cordyceps mycelium powder, add pure water according to the material-liquid ratio of 1:10, control the ultrasonic extraction time for 30 minutes, filter, and use 50% and 75% ethanol water to extract each Once, the filtrates were combined, dried under reduced pressure to remove ethanol to obtain an aqueous extract of Cordyceps mycelium, and then 70 g of maltodextrin was added and mixed well to obtain an aqueous solution of Cordyceps mycelium maltodextrin.

The prepared propolis extract is uniformly added to the prepared Cordyceps hyphae maltodextrin aqueous solution under the action of magnetic stirring, fully mixed, and the temperature is controlled at 40 degrees. The propolis cordyceps mycelium aqueous solution is filtered and freeze-dried to obtain the propolis cordyceps mycelium powder.

Preparation of the test drug: Propolis Cordyceps mycelium powder was prepared with distilled water to a concentration of 1.25g/mL, 2.5g/mL, and 5g/mL.

Experimental design: The experiment set up three propolis Cordyceps dosage groups, a propolis control group and a distilled water control group. Low, medium and high doses of propolis and Cordyceps mycelia were 0.5, 1.0, 2.0 g/kg body weight respectively. The dose of propolis control group was 2.0 g/kg body weight of propolis powder, and the distilled water control group was fed distilled water 20 mL/kg body weight daily.

Experimental animal: ICR mouse, female, clean grade, body weight 18~22g

experimental method:

1. Determination of serum hemolysin in mice: Each group was given samples by intragastric administration, once a day, for 10 consecutive days. On the sixth day of the experiment, each mouse was injected intraperitoneally with 0.2 mL of 2% (V/V) packed sheep red blood cell suspension for immunization. After 4 days, the blood was taken and centrifuged, and the serum was collected. The serum was diluted with normal saline, and incubated in a 37-degree incubator for 3 hours. The degree of hemagglutination was observed, and the number of anti-volume was calculated.

2. Effects on the phagocytic function of peritoneal macrophages in mice: each group was intragastrically administered samples, once a day, for 30 consecutive days. 40 minutes before the animal was sacrificed, each mouse was intraperitoneally injected with 20 (V/V) 1ml of chicken red blood cell suspension, and then injected with 2ml of normal saline, and the peritoneal fluid was taken, incubated in a 37°C incubator for 30 minutes, fixed and stained, and examined under a microscope , count 100 macrophages, and calculate the phagocytosis rate and phagocytosis index.

3. Dinitrofluorobenzene-induced delayed type hypersensitivity test in mice: each group was given samples by intragastric administration, once a day, for 30 consecutive days. 24 hours of hair removal, the area is about 3amX 3am). After 5 days, 10 μl of dinitrofluorobenzene solution was applied to the right ear of the mouse for challenge, and the animal was sacrificed 24 hours later, the ear shell was cut off, and the ear piece with a diameter of 8 mm was removed with a punch and weighed.

Experimental results:

1. Effects on serum hemolysin in mice: Compared with the negative control group (distilled water), the low, medium and high dose groups of propolis and propolis Cordyceps can increase the anti-volume level of serum hemolysin in mice, and the medium and high dose groups of propolis and propolis Cordyceps The effect is very significant. It can also be seen from the experimental results that the middle and high dose groups of propolis and Cordyceps have more significant effects than the propolis single dose group, indicating that the combination of propolis and Cordyceps has a synergistic effect (Table 1).

  

2. Effects on the phagocytic function of mouse peritoneal macrophages: Compared with the negative control group (distilled water), the low, medium and high dose groups of propolis and propolis Cordyceps can improve the phagocytic function of mouse peritoneal macrophages, and the medium and high doses of propolis and propolis Cordyceps The effect of the above two dosage groups is extremely remarkable. It can also be seen from the experimental results that the middle and high dose groups of propolis and Cordyceps are more effective than propolis alone, indicating that the combination of propolis and Cordyceps has a synergistic effect (Table 2).

3. Effect of dinitrofluorobenzene-induced delayed hypersensitivity in mice: Compared with the negative control group (distilled water), the low, medium and high dose groups of propolis and propolis Cordyceps can increase the effect of dinitrofluorobenzene-induced delayed hypersensitivity in mice. For allergies, the effect of propolis and propolis Cordyceps medium and high doses can be very significant. It can also be seen from the experimental results that the medium and high dose groups of propolis and Cordyceps are more effective than propolis alone, indicating that the combination of propolis and Cordyceps has a synergistic effect (Table 3).

in conclusion:

Propolis and propolis cordycepin compound can enhance the function of antibody-producing cells in immunocompromised mice, increase the content of hemolysin, enhance the function of mononuclear phagocytes, improve the delayed hypersensitivity of dinitrofluorobenzene-induced mice, and the compound propolis and cordyceps It is better than the effect of propolis, indicating that the compatibility of propolis and Cordyceps can significantly enhance the body's cellular immunity and humoral immunity.

Example 4 Anti-aging activity in vivo

1. Preparation of the test drug: select 520 grams of natural fresh propolis, freeze and crush it into fine powder, mix it with drinking purified water and edible alcohol respectively to form solutions with alcohol content of 95%, 80%, 70%, 50% concentration and pure Ultrasonic extraction of propolis from water. The ratio of propolis to extraction solutions with different concentrations of alcohol content is 1:4, ultrasonically extracted for 40 minutes, and the filtrates are combined after solid-liquid separation.

Select 400 grams of Cordyceps mycelium powder, add pure water according to the ratio of material to liquid 1:10, control the ultrasonic extraction time at 45 minutes, filter, and use 50% and 75% ethanol water to extract each of the filter residues respectively by ultrasonic in the ratio of 1:4. After 30 minutes, the filtrates were combined, dried under reduced pressure to remove ethanol to obtain an aqueous extract of Cordyceps mycelium, and then 80 g of maltodextrin was added and mixed well to obtain an aqueous solution of Cordyceps mycelium maltodextrin.

The prepared propolis extract is evenly added to the prepared Cordyceps hyphae maltodextrin aqueous solution under the action of magnetic stirring, fully mixed, and the temperature is controlled below 40 degrees. The propolis cordyceps mycelium aqueous solution is filtered and freeze-dried to obtain the propolis cordyceps mycelium powder.

2. Grouping and Modeling

The mice were randomly divided into 6 groups according to body weight: normal group, model group, propolis group, propolis and Cordyceps mycelium powder group with low, medium and high doses. There were 10 rats in each group, and they were raised in a cage of 5 rats and fed a normal diet.

Except for the normal group, the mice in each group were subcutaneously injected with 0.15 g kg-1 D-galactose saline solution on the back of the neck every morning, and the normal group was injected with the same volume of normal saline. After the injection, the mice in each administration group were gavaged with 0.1 mL 10g-1 body weight dose, and the administration doses were: propolis group (1 g kg-1), propolis Cordyceps mycelia low-dose group (0.1 g kg-1 ), medium dose of propolis and Cordyceps mycelium (0.5 g kg-1), high dose of propolis and Cordyceps mycelium (1 g kg-1). Mice in the normal group and the model group were given equal volumes of distilled water.

The activity, appearance, and feces of the mice were observed and recorded every day, and the dosage was adjusted according to the body weight, and the administration was continued for 56 days.

2.1 Anti-aging indicators

The anti-aging activity of the optimized products of propolis and propolis Cordyceps mycelium powder was measured by the external aging and internal aging characteristics of model mice. Among them, the external aging characterization includes the body weight index and behavioral index of the mouse, and the internal characterization includes the organ index and antioxidant index of the mouse.

2.2 BMI

The mice in each group were weighed every 3 days, and the modeling situation was recorded and observed. Each measurement was weighed before feeding at the same time in the morning.

2.3 Behavioral indicators

Behavioral indicators include three parts: the determination of autonomous activity ability, platform jumping test, and the examination of the activity ability and learning ability of mice.

(1) Determination of autonomous activity ability

Voluntary activity was measured every two weeks. In a dark, low-sound environment, the mice were placed in independent activity boxes of the autonomic activity instrument, and waited for 1 min to allow them to adapt. After the adaptation period, the computer automatically recorded the activity of the mice, and measured the number of activities of each mouse within 5 min. Five mice in the same cage were measured at the same time.

(2) Learning ability test

In a dark, low-acoustic environment, five mice in the same cage were placed in separate measurement boxes. The mice received electric shocks at the bottom, and jumped onto the platform to escape the electric shock and acquire memory. For animals with aging or memory impairment, the learning and memory ability will decline within a certain period of time, and the number of jumps will increase. Re-measure after 24 hours, record the number of times the mouse was shocked by electric shocks within 5 minutes and the time when the mouse jumped off the platform for the first time (latency period). If the mouse did not jump off the platform within 5 minutes, the latency period was calculated as 300 s . During the test period, a platform test was carried out every two weeks to observe the improvement effect of each group of drugs on the non-spatial learning and memory ability of aging mice.

2.4 Antioxidant indicators

Blood was collected from the orbit of mice, centrifuged at 3000 rpm for 10 min, and serum was separated. After the body tissue was weighed, 10% tissue homogenate was prepared by adding pre-cooled normal saline according to the weight to volume ratio, centrifuged at 3000 rpm for 15 min, and the supernatant of the tissue homogenate was diluted to the required concentration with normal saline, and stored at -80°C , to be tested.

The mouse tissue protein content, serum and tissue SOD, GSH-px, CAT activities, and the content of MDA in the tissue were measured.

3. Experimental results

After the mice were subcutaneously injected with D-galactose, their body weight gradually slowed down. From the 4th week of modeling, compared with the normal group, the body weight of the model mice began to show a significant difference, and the difference gradually increased with the prolongation of time, and reached a very significant difference by the 5th week. At the beginning of the 4th week of modeling, the body weight of the mice in the propolis Cordyceps high-dose group was significantly different from that in the aging model group. Moreover, in the last week of modeling, the body weight of the mice in the low-dose propolis and cordyceps mycelium complex group still maintained a good upward trend, indicating that administration of low doses of propolis and cordyceps mycelium complex can intervene in D-galactose-induced aging. The weight loss of mice.

After the mice were subcutaneously injected with D-galactose, the activity frequency decreased gradually, the action was sluggish, and the response was unresponsive. By measuring the ability of autonomous activities, it was found that the number of autonomous activities of the mice in the model group within 5 minutes showed a significant decline in 2 weeks after modeling. At 2 weeks after modeling, the spontaneous activity frequency of the mice in the propolis group and the low-, medium-, and high-dose groups of propolis and Cordyceps mycelium powder had a significant difference from the model group, and the gap was further widened in the 6th week of modeling ( Table 4). It shows that propolis and propolis Cordyceps mycelium powder have the effect of resisting the decline of activity caused by aging, and this effect is stable for a long time.

    

At the 4th week of modeling, the model mice showed a significantly higher number of platform errors than normal mice and a significantly lower latency than normal mice. It shows that compared with normal mice, the memory ability of model mice has been significantly decreased, and has the external signs of aging. Propolis and propolis Cordyceps mycelium complex mice showed a significantly lower number of platform errors than model mice and a significantly higher latency than model mice at the 4th week of modeling (Table 5). The performance of the propolis Cordyceps mycelium complex high dose group reached a very significant difference level. It shows that the propolis Cordyceps mycelium complex has the effect of improving the non-spatial long-term learning and memory ability of aging mice.

The results of the determination of SOD activity in mouse serum and tissue (Table 6) showed that the serum SOD activity of mice in the model group decreased significantly. Administration of propolis and propolis Cordyceps compound can significantly improve the activity of serum SOD in mice (p<0.05). Among them, the propolis Cordyceps high-dose group performed better than the propolis control group, and reached a significant difference (p<0.01) compared with the model group, and even reached a significant difference (p<0.05) compared with the propolis group.

The SOD activity in the liver of the model mice also decreased significantly, compared with the normal group, there was a very significant difference (p<0.01). Administration of propolis, propolis Cordyceps medium and high dose groups can significantly increase the activity of SOD in the brain tissue of mice (p<0.05).

The results of this study show that the compound mixture of propolis and propolis Cordyceps has strong anti-aging activity in vivo, can intervene in the weight loss of mice induced by D-galactose, and resist the activity of mice induced by D-galactose In the case of decline, the non-spatial long-term learning and memory ability of mice can be improved, so that they can maintain the memory of stimuli, have a good learning ability to avoid harm, and can improve the activity of SOD in mouse serum and liver. Therefore, the compound mixture of propolis and propolis and Cordyceps can improve the external and internal signs of aging in mice, and the medium-dose group of propolis and Cordyceps compound formula is better than the propolis group, and Cordyceps has a significant synergistic effect on the anti-aging function of propolis.

Claims (4)

1. A propolis and Cordyceps mycelium health food composition, is characterized in that, is made of the raw material of following percentage by weight: propolis 45%-60%; Cordyceps mycelium powder 30-45%, maltodextrin 7- 10%, the sum of the content percentages of each raw material is equal to 100%; the composition is prepared through the following technical scheme: (1) select the propolis raw material according to the weight percentage, use 95%, 75% and 50% aqueous ethanol and Ultrasonic extraction of propolis with pure water according to the solid-liquid ratio of 1:4, the ultrasonic extraction time is controlled at 30-45 minutes, the extracted filtrates are combined to obtain propolis extract; (2) the raw material Cordyceps mycelium powder is mixed with pure water According to the ratio of material to liquid 1:10 ultrasonic extraction 2 times, the time of ultrasonic extraction is controlled at 30-45 minutes, filter, filter residue is respectively ultrasonically leached once with 50% and 75% ethanol aqueous solution according to the ratio of material to liquid 1:4, after extraction The filtrates are combined, dried under reduced pressure to remove ethanol to obtain the water extract of Cordyceps mycelium, then add maltodextrin with a mass ratio of 7-10%, and mix well to obtain an aqueous solution of Cordyceps mycelium maltodextrin; (3) Stir with the aid of magnetic force Add the prepared propolis extract evenly to the prepared Cordyceps mycelia maltodextrin aqueous solution, mix thoroughly for 30 minutes, and control the temperature at 40 degrees; (4) Filter the mixed solution and dry it under reduced pressure Or the freeze-drying method makes the propolis Cordyceps mycelia dry matter. 2. a kind of propolis Cordyceps mycelium health food composition according to claim 1, is characterized in that, the total flavonoid content in the propolis Cordyceps mycelia dried product prepared in the preparation method is not less than 4.0%, and polysaccharide is not less than 4.0%. Less than 6%, adenosine not less than 0.01%. 3. the application of a kind of propolis Cordyceps mycelium health food composition according to claim 1 in the preparation of propolis Cordyceps mycelia health product. 4. application according to claim 3, is characterized in that, described health care product is made by adding appropriate amount of adjuvant with propolis cordyceps mycelia dry matter, and described dosage form is tablet, capsule, granule, drop or oral solution.