CN105410346A - Cultured penaeus vannamei survival rate improving traditional Chinese medicine preparation and preparation method thereof - Google Patents

Abstract

The invention discloses a traditional Chinese medicine preparation for improving the survival rate of cultured prawns, which comprises the following traditional Chinese medicine components in parts by weight: mother of fire: 30-40 parts; gardenia jasminoides: 20-30 parts; Leaf: 15-25 parts; Mountain Yinhua: 15-25 parts; Purslane: 15-25 parts; Paeoniae Rubra: 20-30 parts; Paeonol: 20-30 parts; Malt: 20-30 parts; soluble auxiliary materials : 35-45 servings. By adding Chinese herbal medicine additives processed by modern pharmaceutical technology, which are processed by modern pharmaceutical technology, with Huotanmu, Shanzhizi, Yinchen, Daqingye, Liqun, purslane, red peony, paeonol and malt, it has the effect of clearing away heat. The effects of promoting dampness, detoxifying and protecting the liver, invigorating the stomach and eliminating food, scientific breeding experiments have shown that it can significantly increase the survival rate of Penaeus vannamei. After use, the highest survival rate of Penaeus vannamei can reach 98%, which can significantly promote the growth of Penaeus vannamei , At the same time, it can significantly reduce the ammonia nitrogen value of the water body. More importantly, the Chinese herbal medicine will not bring side effects to the prawns and the feeding environment, and the raw materials of the Chinese herbal medicine of the present invention are cheap and easy to obtain.

Description

Traditional Chinese medicine preparation for improving survival rate of cultured prawns and preparation method thereof

technical field

The invention relates to the technical field of marine product cultivation, in particular to a traditional Chinese medicine preparation which significantly promotes the growth of prawns and improves the survival rate of cultured prawns and a preparation method thereof.

Background technique

Prawn is a kind of rare seafood shrimp. There are many species produced in the oceans all over the world, including Penaeus monodon, Penaeus moji, Penaeus vannamei, and Penaeus shortgroove. Prawns are very popular because of their rich nutrition, soft meat and easy digestion. Due to the depletion of marine fishery resources, people's demand for prawns is increasingly dependent on artificial farming. Therefore, the worldwide prawn farming industry is developing rapidly. In mariculture, the added value of shrimp farming is relatively large, but the risk of farming is also relatively high. This is because during the breeding process of shrimp, it is very easy to suffer from changes in climate, water quality, feeding feed, feeding methods and common diseases. Factors affect the growth of prawns, resulting in low survival rate of prawns. Traditional drug administration methods have relatively large adverse effects. For example, antibiotics have certain curative effects in the treatment of common diseases of Penaeus vannamei, but they have relatively large side effects, such as drug resistance, accumulation in the body, and damage to the ecological balance of water bodies. Quality drops. The following Chinese patents on the use of traditional Chinese medicine ingredients as feed additives in shrimp farming were retrieved:

Application number: 200710059732.8; Applicant: Tianjin Agricultural College; Inventors: Xing Kezhi, Chen Chengxun, Bai Dongqing, Guo Yongjun; Invention name: Traditional Chinese medicine composition for feeding Penaeus vannamei; Abstract: The present invention belongs to the field of feed additive drugs, especially Penaeus vannamei The field of Chinese herbal medicine for feeding. The traditional Chinese medicine composition for raising Penaeus vannamei mainly includes the following components, which are combined in the following weight ratios: Chuanduan 10-15, Atractylodes macrocephala 15-25, Baishao 10-18, Ligustrum lucidum 10-15, rhubarb 10-15 , honeysuckle 8-15, Astragalus 8-15, Bupleurum 3-10, the present invention can increase the content of lysozyme, hemolymph phagocytosis, and improve the levels of superoxide dismutase (sod) and hydrogen peroxide dehydrogenase (cat) , to avoid the shortcomings of antibiotics that are not comprehensive enough and have certain side effects when used in large quantities, the effective rate of preventive use can reach 100%, and the highest survival rate of Penaeus vannamei after use can reach 73%.

Application number: 201510400768.2; Applicant: Bian Jialin; Invention name: Feed additive and feed preparation method of Penaeus prawns containing traditional Chinese medicine ingredients; Abstract: The invention discloses a traditional Chinese medicine additive, which includes the following components in parts by weight: 6-18 parts of Artemisia annua, 5-13 parts of Cyperus cyperus, 10-20 parts of Eupatorium, 5-15 parts of Scopolamine, 4-12 parts of Dawanhua, 3-9 parts of Popona, 10-20 parts of Kidney Fern, 10-20 parts of Haijinsha, 6-18 parts of pangolin, 5-13 parts of mole cricket, 4-12 parts of Xiangqiu vine, 6-18 parts of Niubian, 5-15 parts of mountain lotus leaf, and 5-10 parts of tile grass. The invention also discloses the prawn feed containing the above-mentioned traditional Chinese medicine and a preparation method thereof. The traditional Chinese medicine additive in the feed of the present invention can be used to improve the immunity of Penaeus Moji, and prevent Microsporidiosis of Penaeus Moji, so that the yield is reduced. In addition, the sweet potato vine and pine needle powder in the feed of the present invention can also supplement Moji Trace elements and nutrients needed by prawns.

Application number: 201410326350.7; Applicant: Nanning Ronggang Biotechnology Co., Ltd.; Invention name: A preparation method of a traditional Chinese medicine feed additive for prawns; Abstract: This invention discloses a preparation method of a Chinese medicine feed additive for prawns. The weight ratio of traditional Chinese medicine commonly used in Chinese medicine feed additives is: Rhizoma Polygonatum 17-24 parts, Grass sinensis 15-19 parts, Codonopsis 15-19 parts, Astragalus 13-18 parts, Atractylodes 8-14 parts, Radix Radix 8-13 parts, Andrographis paniculata 8 -13 copies. Mix the medicinal materials in proportion and process them into medicinal powders of 50-70 meshes. Add 30% pure water and appropriate amount of brown sugar to the medicinal powders to ferment naturally into enzymes. After extracting the enzyme liquid, the remaining medicinal residues are ground into a paste by a high-speed refiner; The paste is fully mixed with the purified high-quality bacillus and high-quality yeast and fermented again for 36 hours to make the traditional Chinese medicine feed additive for prawns of the present invention. The invention has the advantages of mild medicinal properties, easier digestion and absorption, good effect of preventing shrimp diseases and no pollution to aquaculture water.

Application number: 201510401431.3; Applicant: Bian Jialin; Invention name: A Chinese medicine additive for Chinese prawn feed; Abstract: The invention discloses a Chinese medicine additive, which includes the following components in parts by weight: Adenophora 12-20 10 to 20 parts of Xu Changqing, 10 to 20 parts of Artemisia annua, 5 to 15 parts of Manjing, 4 to 12 parts of Long Song Luo, 3 to 9 parts of dog ridge fern, 10 to 20 parts of tortoiseshell, 10 to 20 parts of praying mantis, 6-18 parts of buttercup, 5-13 parts of podophyllum, 4-12 parts of ephedra, 6-18 parts of Yizhi, 5-15 parts of ginger Sanqi, and 5-10 parts of canna. The invention also discloses a Chinese prawn feed containing the above-mentioned traditional Chinese medicine additive and a preparation method thereof. The Chinese medicine additive in the feed of the present invention can be used to improve the immunity of Penaeus chinensis, prevent the viral diseases of Penaeus chinensis, and reduce the yield. In addition, the sweet potato vine, clam shell powder and pine needle powder in the feed of the present invention can also be additionally supplemented Trace elements and nutrients needed by Chinese prawns.

But at present there is no document disclosing traditional Chinese medicine preparations made from traditional Chinese medicine raw materials such as Fotanmu, Gardenia jasminoides, capillary, Daqingye, red peony root, paeonol and malt, and are used to promote the growth of prawns, improve the survival rate of prawns, Patents on reducing ammonia nitrogen in water. The traditional Chinese medicine preparation developed by the present invention has the functions of clearing away heat and promoting dampness, detoxifying and protecting the liver, and invigorating the stomach and eliminating food. Scientific breeding experiments have shown that it can significantly improve the survival rate of Penaeus vannamei, and the highest survival rate of Penaeus vannamei after use can reach 98%, significantly promoting the growth of Penaeus vannamei, and at the same time significantly reducing the ammonia nitrogen value of the water body.

Contents of the invention

The technical problem to be solved by the present invention is to provide a traditional Chinese medicine preparation that can significantly promote the growth of prawns, improve the survival rate of cultured prawns, and reduce ammonia nitrogen in water bodies and a preparation method thereof.

The present invention takes following technical scheme:

The traditional Chinese medicine preparation for improving the survival rate of cultured prawns comprises the following traditional Chinese medicine components in parts by weight:

Fotan mother: 30-40 parts; Gardenia: 20-30 parts; Capillary: 20-30 parts; Daqingye: 15-25 parts; Mountain Yinhua: 15-25 parts; parts; red peony: 20-30 parts; paeonol: 20-30 parts; malt: 20-30 parts; soluble excipients: 35-45 parts.

The analysis of each effective component of the traditional Chinese medicine preparation medical material formula of improving the survival rate of cultured prawns mentioned above:

Fotan Mother, [Latin scientific name] PolygonumChinenseL .; [alias] Fotan Star, Blind Gengcane, Tianshi Yin, Fake Sugar Plum, Turtle Dove Rice, Hairy Sugarcane. 【Efficacy】clearing away heat and detoxification, promoting dampness and eliminating stagnation, cooling blood and relieving itching, brightening and relieving nebula. [Indications] For dysentery, dyspepsia, hepatitis, cold, tonsillitis, pharyngitis, diphtheria, whooping cough, corneal clouding, mastitis, fungal vaginitis, leucorrhea, furuncle, pustule in children, eczema, snake bites. [Medicinal value] 1. Antibacterial effect: The antibacterial efficacy of this decoction against Shiga, Schmitz, Flexneri and Shigella sonnei are 1:512, 1:128, 1:64, 1:15 respectively . In addition, the decoction of this product also has a strong antibacterial effect on Staphylococcus aureus, Streptococcus B, Bacillus anthracis, Bacillus diphtheria, Bacillus subtilis, Bacillus typhi, Pseudomonas aeruginosa and Haemophilus influenzae. 2. Antiviral effect: The decoction of this product has been tested for antiviral activity outside the chicken embryo. The antiviral titers against Asian influenza A virus and parainfluenza I virus are 1:160 and 1:64 respectively. Not obvious. 3. Other effects: 95% ethanol extract of this product has antihypertensive effect on rats with renal hypertension. The active ingredient 3,3′-dimethyl gallic acid has a significant effect on cardiac contractility and blood pressure when administered to conscious renal hypertensive rats [3]. Ames test shows that the water extract of this product has a certain anti-mutagenic effect , The mutagenesis inhibition rate is above 10%. In addition, this product has an inhibitory effect on experimental animal tumors, and the gelatin cellulose has a hemostatic effect.

Gardenia jasminoides, [Latin scientific name] Gardenia jasminoides Ellis; [alias] yellow gardenia, yellow fruit tree, red branch; it is the dry and mature fruit of Rubiaceae Gardenia jasminoides Ellis, which belongs to the first batch of medicinal and edible resources promulgated by the Ministry of Health. Liver protection, choleretic, blood pressure lowering, sedation, hemostasis, swelling and other effects. In traditional Chinese medicine, it is often used to treat jaundice hepatitis, sprains, high blood pressure, diabetes and other diseases. [function cures mainly] heat-clearing, purging fire, cooling blood. Treatment of febrile disease, restlessness, jaundice, gonorrhea, thirst, red eyes, sore throat, hematemesis, epistaxis, dysentery, hematuria, toxic heat sores, sprains and swelling. ① "The Classic": "maintain five internal evils, heat in the stomach, flushed face, wine blisters and nose, white leprosy, red leprosy, sores.""Bielu":"Treating eye heat and pain, chest, heart, large and small intestine heat, heart distress, and heat in the stomach." ④ "Medicine Properties": "Killing weed poison, removing heat and wind, benefiting five stranguria, and controlling the middle Evil, relieves urination, relieves five kinds of jaundice, improves eyesight, treats seasonal diseases and eliminates heat and thirst, dry mouth, red eyes, swelling and pain." ⑤ "Diet Therapy Materia Medica": "Mainly dullness, purpura, jaundice with accumulated heat and restlessness. ⑥ "Medical Qiyuan": "Healing the heart meridian guest heat, removing irritability, removing the deficiency heat of the Shangjiao, and treating wind." Stagnant and difficult to urinate." 8. Zhu Zhenheng: "Purge the fire of the triple burner, clear the blood in the stomach and stomach, control the heartache caused by heat, relieve heat and stagnation, and promote stagnation of qi." 8. "Compendium": "Treating hematemesis, epistaxis, dysentery, and bleeding , bloody stranguria, injury and blood stasis, and typhoid overwork, fever, headache, hernia, soup and fire injury." ⑩ "Compendium of Materia Medica": "Use raw fire to purify fire, stir-fry black to stop bleeding, stir-fry ginger juice to treat nausea, internal heat use kernels , Exterior heat with skin." ⑾Guangzhou army "commonly used Chinese herbal medicine handbook": "clearing heat and detoxification, cooling blood and purging fire. Treat jaundice hepatitis, broad bean yellow, cold high fever, bacillary dysentery, nephritis edema, epistaxis, mouth sores, mammary glands Inflammation, sore swollen toxin.” Gardenia jasmine also has antimicrobial effect, that is, gardenia water diffuse liquid can inhibit various fungi such as Pseudomonas schlensi, Epidermophyton inguinale, and Epidermophyton rubrum in the test arm Effect, its water decoction 15 mg/ml can kill Leptospira, in vitro, gardenia decoction can stop the activity of schistosomiasis, and the decoction has no inhibitory effect on bacterial growth. Gardenia alcohol extract stimulates the isolated intestinal smooth muscle of rabbits and rats at low concentrations and inhibits at high concentrations. Didhydroxygeniposide has a laxative effect on mice, and its extract can be made into ointment, which can accelerate the healing of soft tissues.

Capillaris, [Latin scientific name] ArtemisiacapillarisThunb ; [alias] Mian capillaris, Artemisia capillaris, Artemisia capillaris, Artemisia velvet, monkey hair. [nature and flavor] bitter, pungent, slightly cold. [return through] returns spleen, stomach, liver, gall bladder channel. [Chemical composition] Artemisia basilicum contains volatile oil, and the components in the oil include carvone, p-cymene, limonene, perillene, α-phellandrene, thymol, α-, β-pinene, pine oil Alcohol-4, verbenone, naphthalene, arylmethyl acetophenone. It also contains basin, p-hydroxyacetophenone and chlorogenic acid, etc. Bacteria contains volatile oil, and the oil contains myrcene, limonene, eucalyptus Olein, α-pinene, camphene, α-curcumene, dalvatone, caprinone, eugenol, isoeugenol, naphthalene, benzaldehyde, borneol. Also contains caprene chromone, 6, 7-dimethoxycoumarin (ie, artemisinin). It is also reported that artemisinin A and B can be obtained from the aerial part of Artemisia annua. [functions and indications] clearing damp-heat, reducing jaundice. For jaundice oliguria, eczema itching; infectious jaundice hepatitis. In vitro tests have shown that Yinchen decoction is effective against Staphylococcus aureus, Bacillus diphtheria, Bacillus anthracis, Bacillus typhi, Bacillus paratyphoid A, Pseudomonas aeruginosa, Escherichia coli, Shigella flexneri, Shigella dysenteriae, and meningitis. Cocci, Bacillus subtilis, etc. have different degrees of inhibition. 10% decoction can completely inhibit the growth of Mycobacterium tuberculosis. The concentration of 1:100 has inhibitory effect on human and bovine Mycobacterium tuberculosis. The in vitro test of chromogenone of capillary has antagonistic effect on the antibacterial effect of chloramphenicol. Diynone has a high antibacterial effect, especially for skin pathogenic filamentous bacteria, showing a bactericidal effect. After a long time of high temperature treatment above 100 ℃, its antibacterial effect is still not reduced. At a concentration of 0.25 μg/ml, it can also completely prevent the growth and reproduction of Trichophyton rubrum. Artemisinin has anti-inflammatory effect on edema induced by murine carrageenan. Both the acetic acid writhing method and the hot plate method have been shown to have an analgesic effect. Yinchen can promote the division of white blood cells, increase the number of white blood cells, improve the immune activity of T cells, participate in the immune regulation of the body and induce interferon, thus improving the immune function of the body in many ways. The caffeic acid in capillary can increase the number of white blood cells, promote choleresis and stop bleeding, and have anti-fertility effects. Oral capillary 250mg/kg can significantly enhance the delayed type hypersensitivity of transplanted tumor mice.

Daqingye, [Latin scientific name] Isatisindigotica Fortune ; [alias] blue scorpion leaf, blue leaf, blue indigo leaf, indigo leaf, Daqing; [nature and flavor] bitter; cold. [return through] Liver Channel; Heart Channel; Stomach Channel; Spleen Channel. [functions and indications] heat-clearing, detoxification, cooling blood, hemostasis. Treat febrile diseases with excessive heat and polydipsia, influenza, acute infectious hepatitis, bacillary dysentery, acute gastroenteritis, acute pneumonia, erysipelas, hematemesis, epistaxis, jaundice, dysentery, sore throat, aphtha, carbuncle and swollen toxin. 1. "Bie Lu": "Treat Shiqi headache, severe fever, aphtha.""Blue leaf juice, kill hundreds of drug poisons, detoxify wolf poison, shoot dead poison." It is good to treat heat and poison in time." Blue juice, to detoxify. Apply the juice to the five hearts to relieve boredom. Very therapeutic bee and crab venom. 3. "Compendium": "Main heat and toxic dysentery, jaundice, sore throat, erysipelas.""" Blue leaf juice, detoxification of mylabris, coriander, jasmine chicken, cinnabar, arsenic stone poison. " 4, " book on Chinese herbal medicine is just ": " controls plague pyretic poison to go mad, wind-heat macule, carbuncle sore swelling and pain, removes polydipsia, stops epistaxis, spits blood, kills malnutrition, golden sore curare." For those who are both hot and poisonous, it is suitable to use blue leaf pounded juice. "5, Guangzhou Army "Common Chinese Herbal Medicine Handbook": "clearing heat and purging fire, cooling blood and detoxification, dissipating blood stasis to stop bleeding." Treat enteritis, bacillary dysentery, pharyngitis, tonsillitis, mumps, cold and fever, bleeding gums. 6. "Jiangxi Herbal Medicine": "Treating acute hepatitis, tuberculosis, silicosis, toothache, snakebite, and allergic dermatitis." [Pharmacological action] 1. Anti-pathogenic microorganism action: in vitro experiments show that Folium Folium has broad-spectrum antibiotic action. Its decoction has a certain degree of inhibitory effect on Staphylococcus aureus, Streptococcus A, Neisseria meningitidis, Streptococcus pneumoniae, catarrhal bacteria, typhoid bacillus, Escherichia coli, influenza bacillus, diphtheria bacillus and Shigella. Roadside Qingye decoction has bactericidal effect on a variety of Shigella in test tubes; the test also shows that it has good antibacterial effect on Shigella sensitive or resistant to synmycin, nitrofurazone, sulfathiazole, and berberine. effect. Daqingye also has inhibitory effect on Japanese encephalitis virus, mumps virus and influenza virus. It also has a killing effect on Leptospira. 2. Anti-inflammatory and antipyretic effects: Polygonum blue leaf decoction 10g/kg fed to rabbits can inhibit local skin inflammation caused by xylene and reduce capillary permeability. 5g/kg intragastric administration can reduce or accelerate the disappearance of formaldehyde-induced foot swelling in rats. Oral administration of 5-10g/kg can significantly reduce the body temperature of rabbits with fever caused by mixed cholera and typhoid vaccines.

Flos Lonicerae, [Latin scientific name] FlosLonicerae Confusae ; [Also known as] Flos Lonicerae Confusae, Flos Lonicerae, Flos Lonicerae, Flos Lonicerae, Flos Lonicerae; [Efficacy and Indications] Clearing heat and detoxification, dispelling wind-heat. For carbuncle and swollen sores, sore throat, erysipelas, toxic pyretic blood dysentery, wind-heat cold, and warm-heat disease.

Purslane, [Latin scientific name] Portulacaoleracea L.; [alias] purslane, five-element grass, long-lived vegetable, five-party grass, melon seed dish, hemp rope dish, purslane, grasshopper; per 100 grams of purslane Fresh young amaranth stems and leaves contain 2.3 grams of protein, 0.5 grams of fat, 3 grams of sugar, 0.7 grams of crude fiber, 85 mg of calcium, 56 mg of phosphorus, 1.5 mg of iron, 2.23 mg of carotene, 0.03 mg of vitamin BI, and 0.11 mg of vitamin E mg, vitamin P0.7 mg, vitamin C23 mg. In addition, it also contains a large amount of norepinephrine, potassium salt and rich citric acid, malic acid, amino acids and alkaloids. Purslane has a strong inhibitory effect on Escherichia coli, Shigella, typhoid bacillus, etc., especially against Shigella, so. Purslane is suitable for people suffering from acute and chronic dysentery enteritis, cystitis, and urethritis (mild urethral deformity is also acceptable). Purslane contains a lot of norepinephrine and a lot of potassium salts, as well as malic acid, citric acid, glutamic acid, aspartic acid, alanine and sucrose, glucose, fructose and so on. Modern pharmacological studies have shown: antibacterial test: it has inhibitory effects on Escherichia coli, typhoid bacillus, and Shigella, especially against Shigella. [Medicinal effects] heat-toxin diarrhea and dysentery; hot stranguria; anuria; red and white vaginal discharge; uterine bleeding; hemorrhoids and blood; sores and carbuncles; erysipelas; , heat-clearing and detoxifying; cooling blood to stop bleeding; toxic heat and bloody dysentery; carbuncle and furuncle; eczema; snake bites; blood in the stool; Inflammation, bleeding hemorrhoids, etc. The whole herb of horse amaranth is rich in vitamin A, which can promote the physiological function of epithelial cells to normalize and promote the healing of ulcer disease. The whole herb of horse amaranth has inhibitory effect on Shigella and typhoid bacillus. Folks often decoct it in water with a single flavor or wash and mash fresh grass. Take a cup of juice and add a little rice wine to treat dysentery. The whole herb of horse amaranth also has inhibitory effect on common pathogenic skin fungi. Wash it with fresh grass and mash it for external application or decoct it with a single flavor, or decoct it with 50g of stinky phoenix leaves. It can treat eczema, rice field skin moxibustion, and cure Venomous snake bites, lymphatic tuberculosis, acute appendicitis, hemorrhoids, etc. In addition, the content of VE in the fresh grass of amaranth is 0.2%, which is 5 times higher than that of pineapple, which helps the human body to resist aging. In southern Anhui, the blanched and sun-dried whole plant is usually soaked in hair for cooking for postpartum women, which can help postpartum recovery and discharge moisture.

Radix Paeoniae Rubra, scientific name: RADIXPAEONIAERUBRA [alias] Radix Paeoniae Rubra, Grass Radix Paeoniae Rubra, Radix Paeoniae Rubra, Radix Paeoniae Rubra, is the dried root of Paeonia actiflora Pall. or Paeoniaveitchii Lynch. [Efficacy] Clearing heat and cooling blood, dissipating blood stasis and relieving pain. It is used for warming and poisoning spots, vomiting blood and epistaxis, conjunctival congestion and swelling pain, liver depression and hypochondriac pain, amenorrhea dysmenorrhea, abdominal pain in the abdomen, injury from falls, carbuncle sores. [Indications] It is used for warming human blood, warming and poisoning spots, vomiting blood and epistaxis, conjunctival congestion, swelling and pain, liver depression and hypochondriac pain, amenorrhea dysmenorrhea, abdominal pain due to addiction, injury from falls, carbuncle and sore.

Danpi is the dry root bark of Paeoniasuffruticosa Andr. , a perennial deciduous shrub of the family Ranunculaceae. [meridian distribution of nature and flavor] cold in nature, bitter in taste; cool; slightly cold. Guixin; Liver; Kidney; Lung Channel. Bitter, pungent, slightly cold. GUIXIN, liver, kidney channel. [Efficacy and Indications] Clearing away heat and cooling blood; promoting blood circulation and dispelling stasis. Warm febrile disease with heat entering the blood system; spotting; vomiting of nosebleeds; hot tears filling the eyes and causing fever in the late stage of pathogenic bacteria; yin deficiency and bone steaming and hot flashes; blood stagnation and amenorrhea; dysmenorrhea; carbuncle sores; tumbling pain; rheumatism heat paralysis. "Compendium of Materia Medica": "Nourishing yin and reducing fire, detoxifying spot poisoning, benefiting throat, clearing blood stagnation in urination. Later generations use yellow tiller to treat phase fire, but they don't know that the power of paeonol is better. The red flower is beneficial, and the white flower is nourishing. , People are also rarely realized, so it is advisable to distinguish them." Modern research shows that the contained paeonol and other glycoside components have anti-inflammatory effects; the methanol extract of Moutan cortex has the effect of inhibiting platelets; Heat, analgesic, antispasmodic and other central inhibitory effects and anti-atherosclerosis, diuretic, anti-ulcer effects. In vitro experiments show that Moutan cortex decoction has strong antibacterial effect on Bacillus subtilis, Escherichia coli, Bacillus typhi, Bacillus paratyphi, Proteus, Pseudomonas aeruginosa, Staphylococcus, hemolytic streptococcus, pneumococcus, Vibrio cholerae, etc. Effect, peony leaf decoction has significant antibacterial effect on Shigella, Pseudomonas aeruginosa and Staphylococcus aureus, and its active ingredient is gallic acid. Moutan cortex, paeonol, paeoniflorin, oxidized paeoniflorin, and benzoylpaeoniflorin were administered to mice respectively, all of which could promote the clearance of intravenously injected carbon particles in the blood, even if the mononuclear macrophage system function was at The low state also has a promoting effect. Microscopic examination shows that the phagocytosis of Kupffer cells in the liver and macrophages in the spleen is enhanced. Paeoniflorin and oxidized paeoniflorin can also enhance the phagocytosis of mouse peritoneal macrophages to emulsion in vitro. Intraperitoneal injection of paeonol solution to mice can increase the number of plaques in the spleen after hemolysis. Intraperitoneal injection of paeonol to mice at 25 mg/kg per day for 6 consecutive days can significantly increase the spleen weight, and can resist the reduction of thymus weight caused by cortisone and cyclophosphamide. It can be seen from the above that Cortex Moutan can enhance both humoral and cellular immunity. Intragastric administration of paeonol can inhibit paw edema in rats caused by dextran, acetic acid or carrageenan, and can inhibit the increase of capillary permeability in mouse abdominal cavity or guinea pig skin caused by acetic acid or 5-hydroxytryptamine , Inhibit the occurrence of stress ulcer in mice.

Malt is obtained by germination and drying of the mature fruit of the gramineous plant barley Hordeumvulgare L. [Latin scientific name] FructusHordeiGerminatus [alias] barley grits, barley wool, barley malt, raw malt, barley tiller, wheat tiller, fried malt, burnt malt, barley glutinous rice flour. [nature and flavor] sweet; flat [return through] spleen meridian; stomach meridian. [Functions and Indications] Promoting qi and eliminating food, invigorating the spleen and appetizing, reducing breast milk and reducing swelling. Used for indigestion, distending pain in the abdomen, lack of appetite due to spleen deficiency, stagnant milk, breast distending pain, and women weaning. Barley alkaloids A and B contained in malt have antifungal activity.

The soluble auxiliary material is any one of dextrin, soluble starch or lactose.

A preparation method of a traditional Chinese medicine preparation for improving the survival rate of cultured prawns, comprising the steps of:

(1) Pre-treatment of raw materials: crush the mother of charcoal, jasmine jasmine, Folium oleracea, purslane, red peony root and paeonol into 40-60 mesh, mix with capillary, mountain silver flower, malt, 80-100 mesh filter bag wrapping;

(2) Extraction of active substances: Add 20 times the amount of water to the filter cloth bag containing the mixed medicine fine particles in step (1), reflux and extract the multifunctional extraction tank twice, boil for 30 minutes each time, filter cloth with 300-500 mesh Filter and combine the filtrates;

(3) Concentration under reduced pressure: Concentrate the filtrate in step (2) under reduced pressure in a single-effect external circulation pot until it reaches a concentrated medicinal solution with a relative density of 1.10-1.30 at 70-80°C;

(4) Add excipients: add soluble excipients and mix well to fully dissolve the excipients and make the excipients highly mixed evenly;

(5) Instant drying: use the method of spray drying to dry the medicine-aid mixture at 210-220°C;

(6) Quality inspection of intermediates: testing the content, solubility and preparation microorganisms of active ingredients;

(7) Packing: 200g/bottle is packed in polyethylene plastic bottles, and the inner cap is heat-sealed with electromagnetic waves.

The method of use of this traditional Chinese medicine preparation: according to the percentage of the medicine powder and the feed is 2% ~ 10%, mix and mix in the feed and feed the prawns, 1 ~ 2 times a day, 3 ~ 7 consecutive days as a cycle.

The beneficial effects of the present invention are:

1. By adding Chinese herbal medicine additives processed by modern pharmaceutical technology, which are made of fotanmu, gardenia, capillary, Folium Isatidis, mountain silver flower, purslane, red peony root, paeonol and malt as raw materials, It has the effects of clearing away heat and promoting dampness, detoxifying and protecting the liver, invigorating the stomach and eliminating food. After the inventor uses the breeding environment of two specifications of the glass shrimp tank and the cement pond respectively, a test group (the traditional Chinese medicine for improving the survival rate of cultured prawns produced by the present invention) is carried out. The inventor named the traditional Chinese medicine preparation as "test drug group") on the survival and growth of Penaeus vannamei and the influence of ammonia nitrogen in the water body. Scientific breeding experiments showed that it can significantly improve the survival rate of Penaeus vannamei. After use, the highest vannamei The survival rate of prawn farming can reach 98%, which can significantly promote the growth of Penaeus vannamei, and can also significantly reduce the ammonia nitrogen value of the water body. More importantly, the Chinese herbal medicine will not bring side effects to the prawns and the feeding environment, and the raw materials of the Chinese herbal medicine of the present invention are cheap and easy to obtain.

2. The traditional Chinese medicine preparation developed by the present invention also has the effects of anti-bacteria, anti-virus, improving immune function, improving the body's disease resistance, and has no pollution to aquaculture water.

3. The inventor has also carried out a test drug group (the traditional Chinese medicine preparation produced by the present invention to improve the survival rate of cultured prawns, the inventor named the Chinese medicine preparation "test drug group") to treat the liver, stomach and intestinal diseases of Penaeus vannamei The curative effect observation test showed that the test drug group had obvious curative effect on the liver, stomach and intestinal diseases of Penaeus vannamei.

detailed description

Example 1

The traditional Chinese medicine preparation for improving the survival rate of cultured prawns comprises the following traditional Chinese medicine components in parts by weight:

Fotan mother: 30 parts; Gardenia: 20 parts; Capillary: 20 parts; Daqingye: 15 parts; Mountain silver flower: 15 parts; parts; malt: 20 parts; dextrin: 35 parts.

A preparation method of a traditional Chinese medicine preparation for improving the survival rate of cultured prawns, comprising the steps of:

(1) Pre-treatment of raw materials: crush 30 parts of Fotanmu, 30 parts of 20 parts of gardenia, 15 parts of Folium oleracea, 15 parts of purslane, 20 parts of red peony and 20 parts of paeonol into 40 meshes, and 20 parts of Yinchen, 15 parts of mountain silver flower, 20 parts of malt, wrapped in 80-mesh filter bag;

(2) Extraction of active substances: add 20 times the amount of water to the filter cloth bag containing the fine particles of the mixed medicine, reflux and extract twice in the multifunctional extraction tank, boil for 30 minutes each time, filter with a 300-mesh filter cloth, and combine the filtrates;

(3) Concentration under reduced pressure: Concentrate the above-mentioned filtrate under reduced pressure in a single-effect external circulation pot to a concentrated medicinal solution with a relative density of 1.10 at 70°C;

(4) Add excipients: Add 35 parts of dextrin and mix well to fully dissolve the excipients and make the excipients highly mixed evenly;

(5) Instant drying: use the method of spray drying to dry the medicine-aid mixture at 210°C;

(6) Quality inspection of intermediates: testing the content, solubility and preparation microorganisms of active ingredients;

(7) Packing: 200g/bottle is packed in polyethylene plastic bottles, and the inner cap is heat-sealed with electromagnetic waves.

Method of use: The method of use of this traditional Chinese medicine preparation: according to the percentage of the medicine powder and the feed is 2%, mix and mix in the feed and feed the prawns, 1-2 times a day, and a cycle of 3-7 days in a row.

Example 2

The traditional Chinese medicine preparation for improving the survival rate of cultured prawns comprises the following traditional Chinese medicine components in parts by weight:

Fotan mother: 35 parts; Gardenia: 25 parts; Capillary: 25 parts; Daqingye: 20 parts; Mountain silver flower: 20 parts; parts; malt: 25 parts; soluble starch: 40 parts.

A preparation method of a traditional Chinese medicine preparation for improving the survival rate of cultured prawns, comprising the steps of:

(1) Raw material pretreatment: crush 35 parts of Fotanmu, 35 parts of 25 parts of Gardenia, 20 parts of Folium Isatidis, 20 parts of Purslane, 25 parts of Radix Paeoniae Rubra and 25 parts of Dan Cortex into 50 meshes, and mix with 25 parts of Capsicum chen, 20 parts mountain silver flower, 25 parts malt mixed, wrapped in 100 mesh filter bag;

(2) Extraction of active substances: Add 20 times the amount of water to the filter cloth bag containing the mixed drug fine particles, reflux and extract twice in the multifunctional extraction tank, boil for 30 minutes each time, filter with a 500-mesh filter cloth, and combine the filtrates;

(3) Concentration under reduced pressure: Concentrate the above-mentioned filtrate under reduced pressure in a single-effect external circulation pot, and reach a concentrated medicinal solution with a relative density of 1.20 at 75°C;

(4) Add excipients: Add 40 parts of soluble starch and mix well to fully dissolve the excipients and make the excipients highly mixed evenly;

(5) Instant drying: use the method of spray drying to dry the medicine-aid mixture at 215°C;

(6) Quality inspection of intermediates: testing the content, solubility and preparation microorganisms of active ingredients;

(7) Packing: 200g/bottle is packed in polyethylene plastic bottles, and the inner cap is heat-sealed with electromagnetic waves.

The method of use of this traditional Chinese medicine preparation: according to the percentage of the medicine powder and the feed is 6%, mix it with the prawns in the feed, 1-2 times a day, and a cycle of 3-7 consecutive days.

Example 3

The traditional Chinese medicine preparation for improving the survival rate of cultured prawns comprises the following traditional Chinese medicine components in parts by weight:

Fotan mother: 40 parts; Gardenia: 30 parts; Capillary: 30 parts; Daqingye: 25 parts; Mountain silver flower: 25 parts; parts; malt: 30 parts; lactose: 45 parts.

A preparation method of a traditional Chinese medicine preparation for improving the survival rate of cultured prawns, comprising the steps of:

(1) Pretreatment of raw materials: crush 40 parts of Fotanmu, 40 parts of 30 parts of Gardenia, 25 parts of Folium Isatidis, 25 parts of Purslane, 30 parts of Radix Paeoniae Rubra and 30 parts of Dan Cortex into 60 meshes, and mix with 30 parts of Yinchen, 25 parts of mountain silver flower, 30 parts of malt, wrapped in a 100-mesh filter bag;

(2) Extraction of active substances: Add 20 times the amount of water to the filter cloth bag containing the fine particles of the mixed medicine, reflux and extract twice in the multifunctional extraction tank, boil for 30 minutes each time, filter through a 350-mesh filter cloth, and combine the filtrates;

(3) Concentration under reduced pressure: Concentrate the above-mentioned filtrate under reduced pressure in a single-effect external circulation pot to a concentrated medicinal solution with a relative density of 1.30 at 80°C;

(4) Add excipients: Add 45 parts of lactose, mix well, fully dissolve the excipients, and make the excipients highly mixed evenly;

(5) Instant drying: use the method of spray drying to dry the medicine-aid mixture at 220°C;

(6) Quality inspection of intermediates: testing the content, solubility and preparation microorganisms of active ingredients;

(7) Packing: 200g/bottle is packed in polyethylene plastic bottles, and the inner cap is heat-sealed with electromagnetic waves.

The method of use of this traditional Chinese medicine preparation: according to the percentage of the medicine powder and the feed is 10%, mix it with the prawns in the feed, 1-2 times a day, and a cycle of 3-7 consecutive days.

Pharmacodynamics test of the present invention:

The inventor used the culture environments of glass shrimp tanks and cement ponds respectively to carry out the test drug group (the traditional Chinese medicine preparation produced by the present invention to improve the survival rate of cultured prawns, the inventor named the Chinese medicine preparation "test drug group") Experiments on the survival and growth of Penaeus vannamei and the effects of ammonia nitrogen in water.

Materials and Methods

1.1 Test material

Shrimp seedlings: bright in color, healthy and vigorous, without trauma, neat in size, no abnormality after 12 hours of water testing, purchased from Hepu shrimp seedling farm.

Feed: Produced by Zhanjiang Keyuan Feed Co., Ltd.

Glass shrimp tank: The size is 40cm×50cm×50cm, with a drain at the bottom.

Cement pool: The specification is 1m×1m×2m, with a drain at the bottom.

Water source: Sterilized fresh seawater in the cistern.

Reagent group: the traditional Chinese medicine preparation produced by the present invention to improve the survival rate of cultured prawns.

Positive medicine of traditional Chinese medicine: Shrimp Fuxing Dan, a marketed preparation composed of Huanglian, Scutellaria, Cortex Phellodendron, and Zongzi.

experiment method

The experiment was carried out from September 15, 2015 to October 23, 2015 at the Penaeus vannamei breeding base of the research group. There are two types of experimental groups: glass shrimp tanks and cement pools.

Glass shrimp tank group: Select 150 shrimp seedlings with a body length of 3±0.5cm after 20 days of emergence, and use a glass shrimp tank with a size of 80cm×60cm×50cm. The bottom of the tank is equipped with a drain port to disinfect the seawater. The bottom of the tank is replaced every day 20% of water, divided into 3 experimental groups: blank group, Chinese medicine positive group, test drug group. 50 shrimp seedlings were thrown into each group, and the blank group was not given medication, and the feed was fed as usual, with 3 meals a day; the drug administration group (Chinese medicine positive group, test drug group) mixed the test drug group in the feed and fed, daily 1 time, 3 consecutive days of administration and 2 days of withdrawal as a cycle. After 6 cycles of administration, count the number of Penaeus vannamei one by one, and randomly select 12 prawns from each tank, weigh each one, and measure each shrimp at the same time. Ammonia nitrogen in tank water.

Cement pool group: Select 300 shrimp seedlings with a body length of 3 ± 0.5cm after 20 days of emergence, use a cement pool with a size of 1m × 1m × 2m, and a drain port is provided at the bottom of the pool to sterilize mariculture, and change the water at the bottom of the pool every day 20%, divided into 3 experimental groups: blank group, positive Chinese medicine group, test drug group. Each group threw 100 shrimp fry. The blank group was not administered, and was fed with feed as usual, 3 meals a day; the drug group (Chinese medicine positive group, test drug group) was fed with the medicine mixed in the feed, once a day, and administered continuously for 3 days, Two days of drug withdrawal constituted a cycle. After 8 cycles of drug administration, the number of Penaeus vannamei was counted pool by pool, and 12 prawns were randomly selected from each tank, weighed one by one, and the ammonia nitrogen in each pool was measured at the same time.

Data Statistical Analysis

Enumeration data, expressed in ±s, the difference between the two groups was tested by t test, and data processing was carried out by SPSS Statistics 17.0 for multiple comparisons. P<0.05 indicated significant difference, and P<0.01 indicated extremely significant difference.

For measurement data, use the χ ² (2×2) method---statistical analysis of the positive rates of the two groups, and use the following formula to calculate x ² ,

When χ ² >6.635, P<0.01; when χ ² >3.841, P<0.05; when χ ² <3.841, P>0.05. P<0.05 means significant difference, P<0.01 means extremely significant difference.

Experimental results

Table 1 shows the survival number, survival rate and body weight of Penaeus vannamei in each group of glass shrimp tanks. The results show that: in the glass shrimp tank experimental group, compared with the blank group, the survival rate and body weight of the test group were significantly improved ( P<0.01); compared with the positive group, there was no significant difference (P>0.05).

Table 2 shows the measurement results of ammonia nitrogen in each group of glass shrimp tanks. The results show that in the glass shrimp tank experimental group, the ammonia nitrogen values in the blank group, the positive group and the test drug group all exceeded the standard limit value of 0.15mg/L, exceeding the limit. The order of the values is: positive group>blank group>test drug group. The results suggest that the test drug group preparation can reduce the ammonia nitrogen value of the water body, but the positive group cannot reduce the ammonia nitrogen value of the water body.

Table 3 shows the results of the survival number, survival rate and body weight of Penaeus vannamei in each group of cement ponds. The results show that: in the cement pond experimental group, compared with the blank group, the survival rate of the test group was significantly increased (P<0.01), and the body weight Significantly increased (P<0.05); compared with the positive group, the survival rate of the test group had no significant difference, but its body weight increased significantly (P<0.05).

Table 4 shows the ammonia nitrogen value of each group of water in the cement pool. Compared with the blank group and the positive group, the ammonia nitrogen of the water in the test group was significantly reduced (P<0.05).

Note: Compared with the blank group, ^* P<0.05; compared with the positive group, ^# P<0.05.

Summary and Discussion

In the glass shrimp tank experimental group and the cement pool experimental group, compared with the blank group, the survival rate of the test drug group increased by 28% and 18% respectively, all of which were very significantly different ((P<0.01). The impact on the survival of Penaeus vannamei is extremely significant, and can significantly improve the survival rate of Penaeus vannamei.

Compared with the blank group, the body weight of the test group in the glass shrimp tank was significantly increased ((P<0.01), and the body weight of the test group in the cement pool was significantly increased ((P<0.05); moreover, the test group in the cement pool The body weight of the group is significantly improved (P<0.05) than the positive group. Show that the test group has a very significant promotion effect on the growth of Penaeus vannamei. By measuring the ammonia nitrogen in each group of water bodies, the order of ammonia nitrogen values from large to small shows that, The test group can significantly reduce the ammonia nitrogen value of the water body.

During the breeding process of Penaeus vannamei, there are many factors that affect its survival and growth, such as climate change, common diseases, water quality, drug intervention, etc. The present invention, from the perspective of drug intervention, develops a medicinal product with the functions of clearing away heat and promoting dampness, detoxifying and protecting the liver. , the test group of the new preparation of traditional Chinese medicine for invigorating the stomach and eliminating food, carried out experiments with the small water body of the glass tank and the medium water body of the cement pool respectively, and investigated the effect of the test group on the survival and growth of Penaeus vannamei, and also investigated its effect on nitrogen and ammonia in the water body. The results showed that the test group significantly improved the survival rate of Penaeus vannamei, and the highest survival rate of Penaeus vannamei after use could reach 98%, which significantly promoted the growth of prawns and significantly reduced the ammonia nitrogen in the water body.

The inventor also carried out the observation of the curative effect of the experimental group (the traditional Chinese medicine preparation produced by the present invention to improve the survival rate of cultured prawns, which the inventor named as "the experimental group") to treat the liver, stomach and intestinal diseases of Penaeus vannamei test.

Test time: 2015.8.25-.29

Test site: the fifth pond of the research group's vannamei breeding base

Methods and results: The fifth pond covers an area of 1 mu. There were 100,000 shrimp juveniles of Penaeus vannamei with a tail length of 5-6cm. On the morning of August 25, 2015, about 20% of the shrimps were found to have red and swollen livers and blurred hepatopancreatic lines. Clearance, jejunum, diarrhea and other symptoms, at noon on August 25, 2015, the Chinese medicine test group was mixed into the prawn feed at 4-5% and fed to the fifth pond, administered once a day for 3 consecutive days, and the result was, From the second day of administration, symptoms such as jejunum and diarrhea gradually disappeared. After 3 days of administration, liver and intestinal symptoms changed significantly and returned to normal. On the 4th and 5th day, the follow-up observation continued, the liver, stomach and intestinal functions of the prawns returned to normal, there was no jejunum and white stool phenomenon, the liver color returned to a healthy color, and the prawn feed was increased from the original 3.0Kg to 3.5Kg, within 1.5 hours finish eating.

Conclusion: This experiment suggests that the experimental group has obvious curative effect on the liver, stomach and intestinal diseases of Penaeus vannamei.

Claims (4)

1. improve the Chinese medicine preparation of cultured prawn survival rate, it is characterized in that: the traditional Chinese medicinal components comprising following parts by weight:

Chinese knotweed: 30-40 part; Fructus gardeniae: 20-30 part; Oriental wormwood: 20-30 part; Folium isatidis: 15-25 part; Honeysuckle flower: 15-25 part; Purslane: 15-25 part; The radix paeoniae rubrathe: 20-30 part; The root bark of tree peony: 20-30 part; Fructus Hordei Germinatus: 20-30 part; Solubility auxiliary material: 35-45 part.

2. the Chinese medicine preparation of raising cultured prawn survival rate according to claim 1, is characterized in that: described solubility auxiliary material is any one in dextrin, soluble starch or lactose.

3. the Chinese medicine preparation of raising cultured prawn survival rate according to claim 1, is characterized in that: the preparation method of this Chinese medicine preparation, comprises the steps:

(1) raw material pre-treatment: Chinese knotweed, fructus gardeniae, folium isatidis, purslane, the radix paeoniae rubrathe and the root bark of tree peony are ground into 40-60 order, mixes with oriental wormwood, Honeysuckle flower, Fructus Hordei Germinatus, and 80-100 order filter cloth bag wraps up;

(2) extraction of active material: filter cloth bag step (1) being equipped with mixing medicine particulate, adds 20 times of water gagings, multi-function extractor refluxing extraction 2 times, each boiling 30 minutes, 300-500 order filter-cloth filtering, merging filtrate;

(3) reduced pressure concentration: by the filtrate of step (2) with single-effect external circulation pot reduced pressure concentration, be the concentrated medicament of 1.10-1.30 to relative density during 70-80 DEG C;

(4) auxiliary material is added: add solubility auxiliary material, mix thoroughly, auxiliary material is fully dissolved, the auxiliary height of medicine is mixed;

(5) wink-dry: adopt spray-dired method, in 210-220 DEG C by auxiliary for medicine mixed liquor wink-dry;

(6) intermediate quality examination: the content of detection of active composition, melting and preparation microorganism;

(7) packing: be sub-packed in vinyon bottle by 200-300g/ bottle, electromagnetic wave heat-sealing inner cap.

4. the Chinese medicine preparation of raising cultured prawn survival rate according to claim 1, it is characterized in that: this Chinese medicine preparation using method: be 2% ~ 10% puddle prawn of throwing something and feeding in feed by the percentage of medicinal powder and feed, every day 1 ~ 2 time, continuous 3 ~ 7 days is a cycle.