CN101757614B - Hemostatic agent and preparation method thereof - Google Patents
Hemostatic agent and preparation method thereof Download PDFInfo
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- CN101757614B CN101757614B CN2010101131153A CN201010113115A CN101757614B CN 101757614 B CN101757614 B CN 101757614B CN 2010101131153 A CN2010101131153 A CN 2010101131153A CN 201010113115 A CN201010113115 A CN 201010113115A CN 101757614 B CN101757614 B CN 101757614B
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Abstract
A hemostatic and its preparation method, wherein each 1000g of finished liquid comprises: 10-16g of termite fungus garden composition powder, 4-6g of collagen, 0.02-0.04g of tribasic acid, 7-9g of bactericide and the balance of water. The advantages are that: the termite fungus garden composition powder containing rich nutrient components is adopted in the formula, so that the composition has the effects of relieving swelling, astringing and stopping bleeding, tonifying deficiency bodies and generating no side reaction; collagen has very strong affinity with different cells, and also has ideal affinity with growth factors playing a key role in the process of wound healing, and after blood coagulation, the collagen can also prevent the occurrence of secondary bleeding by stimulating the regeneration and repair of tissues; the criticality of the using object does not exist; the hemostatic effect is rapid, and the hemostatic bag has excellent anti-inflammatory, detumescence and analgesic effects, and has no adverse reaction such as toxic and side effects and irritation; the preparation method provided has the advantages of few process steps and non-harsh preparation conditions, and can be suitable for industrial mass production.
Description
Technical field
The invention belongs to the surgical medicine technical field, be specifically related to a kind of hemorrhage, be applicable to traumatic hemostasis and surgical hemostasis, and relate to the method for preparing of this kind hemorrhage human body.
Background technology
In daily life; When people run into skin and are frayed by foreign body; Why be accustomed to smearing with saliva, this is because saliva has hemostatic function, and the U.S. scientist Si Tan Koln scholar that the secret of saliva with hemostatic function once obtained Nobel Prize in medicine (1986) opens; This scientist finds that a kind of living cells is arranged in the saliva, contains the polypeptides matter (being called for short cell chemotactic factor < ECF >) of 53 seed amino acids in this living cells.The maximum characteristics of polypeptides matter are the proliferation and differentiation that can promote cell, replace old and feeble with dead cell with new cell.
Just because of this smooth Koln scholar's discovery, make people obtain corresponding inspiration, that is exactly to explore natural fungus garden of Odontotermes formosanus and to use service human.The constituent of fungus garden of Odontotermes formosanus mainly contains lignin, vitamin, soil particle, plant humus, wood fibre, Coptotermes formosanus Shtrari. secretions and Excreta etc.; This abundant organic nutrient substance and on every side mineral and moisture and corresponding environmental condition combine, and have had the condition of termitomyces and symbiosis of termite, commensalism and coexistence.To be the saliva of utilizing oneself owing to Coptotermes formosanus Shtrari. with above-mentioned material be bonded together, and to construct termitarium be fungus garden of Odontotermes formosanus; Therefore; Fungus garden of Odontotermes formosanus contains the following material that has been confirmed by the nutrient source institute analysis of BeiJing, China city: thick protein 9.88-12.17% and trace element; Wherein, contain potassium 0.20%, sodium 0.03%, calcium 1.84%, magnesium 0.27%, ferrum 0.34%, copper 17ppm, manganese 0.13%, zinc 41ppm, selenium 1.0ppm and total flavones 0.19% etc. in the trace element.In addition; Contain vitamin E 5.36mg in the per kilogram fungus garden of Odontotermes formosanus; Amino acid content is in the fungus garden of Odontotermes formosanus of every 100mg: aspartic acid 0.86mg, threonine 0.44mg, green propylhomoserin 0.40mg, glutamic acid 0.81mg, glycine 0.41mg, alanine 0.45mg, valine 0.43mg, methionine 0.07mg, isoleucine 0.56mg, leucine 0.67mg, tyrosine 0.19mg, phenylalanine 0.30mg, lysine 0.29mg, histidine 0.15mg, arginine 0.41mg, proline 0.35mg, tryptophan 0.06mg and cystine 0.20mg, its summation is 7.35mg.
From the above owing to contain abundant hemostasis and thrombin in the fungus garden of Odontotermes formosanus, if thereby can scientific utilization, be made into haemostatic medicament, then can yet be regarded as is a kind of useful act.In disclosed Chinese patent document, although about the technical scheme of hemorrhage 40 pieces of writing nearly, none relates to the teachings of utilizing fungus garden of Odontotermes formosanus.In non-patent literature, include the medicinal technical information of Coptotermes formosanus Shtrari. and fungus garden of Odontotermes formosanus, for example, " Guangxi Chinese medicine " 1996.19 (5) 45-47 pages or leaves have been mentioned the medical value (author: Zhao one) of Coptotermes formosanus Shtrari.; And for example, " Anhui tcm clinical practice journal the 11st the 5th phase of volume of October in 1998 has been introduced fungus garden of Odontotermes formosanus treatment bleeding hemorrhoids 124 routine clinical observations (author: Li Yang is careful, Xie Zujian and river grid etc.); For another example, " Guangxi Chinese medicine institute journal " 1999.2 (2) published the pharmacodynamic study (J) (author: Li Aiyuan and Huang Ruisong etc.) of Macrotermes annandalei Slivestri; Also as, " Chinese crude drug " 2006.29 (10): 1011-1013 has reported antiinflammatory action research (J) (author: Cao Juan, Sun Tao, Zhang Min and Xue Dejun) of fungus garden of Odontotermes formosanus., above-mentioned non-patent literature of giving an example constitutes the teachings of hemorrhage but not providing fungus garden of Odontotermes formosanus and other material combination equally.
At present, the bleeding-stopping dressing that the surgical operation of hospital is used mostly uses gelfoam, because the hydrophilic of this material is poor, so haemostatic effect is unsatisfactory.Though the performance of former gelatin sponge is better than gelfoam, because of same unsatisfactory being difficult to of haemostatic effect in clinical large-scale application.
The applicant has passed through exploration for many years, has found and has utilized the way of fungus garden of Odontotermes formosanus as hemorrhage, and the technical scheme that will introduce below produces under this prerequisite.
Summary of the invention
Task of the present invention is to provide a kind of and has that hemostasis is fast, anti-inflammation detumescence analgesic effect is good, safe without toxic side effect and non-stimulated hemorrhage.
Task of the present invention also is to provide a kind of method for preparing of hemorrhage, and this method processing step is succinct, preparation condition is not harsh, can satisfy the embodiment that industrialized mass production required and ensured the technique effect of said hemorrhage.
Task of the present invention is accomplished like this, and a kind of hemorrhage comprises in the finished product liquor of every 1000g weight: fungus garden of Odontotermes formosanus compositions powder body 10-16g, collagen protein 4-6g, ternary acid 0.02-0.04g and antibacterial 7-9g, surplus is a water.
In a concrete embodiment of the present invention, described fungus garden of Odontotermes formosanus compositions powder body is the mixture of termite population powder body and termite nest fungus comb powder body.
In another concrete embodiment of the present invention, the weight ratio of described termite population powder body and termite nest fungus comb powder body is 1: 15-20.
In another concrete embodiment of the present invention, described Coptotermes formosanus Shtrari. is an Odontotermes formosanus Shiraki, and described fungus garden is the fungus garden that contains termitomyces.
In another concrete embodiment of the present invention, described collagen protein is a medical collagen, and described antibacterial is that mass percent concentration is the hydrogen peroxide of 26-28%.
Also have among the concrete embodiment of the present invention, described ternary acid is the phosphoric acid or the citric acid of SILVER REAGENT, and described water is that purity is deionized water or the distilled water of 0.08-0.12 μ s/cm.
An also task of the present invention is accomplished like this, and a kind of method for preparing of hemorrhage may further comprise the steps:
A) produce fungus garden of Odontotermes formosanus compositions powder body; The termite nest fungus comb dewatered drying that contains termite population that will from Coptotermes formosanus Shtrari. ant road, dig out earlier, pulverize and sieve, sterilization then, reuse dissolution with solvents, filtration and distillation and reclaim solvent; Obtain fungus garden of Odontotermes formosanus compositions powder body, for use;
B) fungus garden of Odontotermes formosanus compositions powder body is added to the water stirs, obtain fungus garden of Odontotermes formosanus composition powder liquid solution;
C) collagen protein powder is added to the water stirs, add antibacterial again and behind 30-35min, stir then, obtain collagen solution;
D) fungus garden of Odontotermes formosanus composition powder liquid solution is joined in the collagen solution, stir, add entry again and then stir, obtain mixed liquor;
E) ternary acid joined in the mixed liquor stir, the pH value of mixed liquor is regulated, through leave standstill, filtration and fill, obtain the finished product hemorrhage.
more of the present invention and among concrete embodiment, steps A) the used device of dewatered drying described in is the constant temperature drying case, and bake out temperature is 80-100 ℃; Drying time is 240-300min; The used device of described pulverizing is a ball mill, described sieving to crossing 100 mesh sieves, and described sterilization is for adopting the co-60 radiation sterilization; The time of radiation sterilization is 180-240min; Described solvent is water or ethanol, describedly is filtered into that to adopt the aperture be the membrane filtration of 10-20 μ m, and described distillation is distilling under reduced pressure.
Of the present invention again more and among concrete embodiment, step C) mixing time that stirs that is added to the water of the collagen protein powder described in is 5-10min, and the described mixing time that stirs then is 30-35min.
In again of the present invention and then concrete embodiment, step D) described in stir with so that the mixing time that stirs respectively be 5-10min; Step e) mixing time that stirs described in is 5-10min, and described pH value is 5-6, and the described time of leaving standstill is 40-60min, and described being filtered into adopted 100 purpose strainer filterings.
One of advantage of the hemorrhage of technical scheme provided by the invention; Owing to adopted the fungus garden of Odontotermes formosanus compositions powder body that contains rich nutrient contents in the prescription; Thereby in detumescence, convergence and hemostatic simultaneously, also have the effect of the empty body of tonification and can not produce any side reaction; Two; In prescription, used collagen protein, because of collagen protein has strong effect hemostatic mechanism, it is as a kind of natural hemorrhage; And very strong affinity is arranged between the different cells; Also have ideal affinity simultaneously and between the somatomedin that plays a crucial role in the wound healing process, behind blood coagulation, collagen protein can also prevent once more hemorrhage generation through histio-irritative regeneration and reparation; Three; There is not the fastidious property of using object in hemorrhage of the present invention; Except general hemostasis situation; Also can be applicable to blood coagulation factor VIII or IX deficient patients (hemophilia A or hemophilia B), light moderate thrombocytopenia and special circumstances such as process aspirin or heparin therapy all can obtain gratifying haemostatic effect; Four, haemostatic effect is rapid, and has excellent antiinflammatory, detumescence and pain relieving effect, has no side effect and non-stimulated and so on untoward reaction; Five, the method for preparing processing step that provides is few, preparation condition is not harsh, can be suitable for industrial-scale production.
The specific embodiment
Through applicant's following detailed description, especially five non-restrictive example explanations through providing will help to understand the present invention more, and can become more clear to advantage of the present invention and effect.
Embodiment 1:
A) produce fungus garden of Odontotermes formosanus compositions powder body, fungus garden of Odontotermes formosanus compositions powder body both can directly obtain from commercially available channel, also can make by oneself; Present embodiment promptly adopts self-control to be made as example certainly, to the termite nest fungus comb chemical examination (check) that contains termite population of from termite runway, digging out that provides from market; Confirm that termite population is the Odontotermes formosanus Shiraki population, and confirm termite nest fungus comb for containing Odontotermes formosanus Shiraki (Odontotermes formosanus<the Shiraki >) main nest bacterial nursery of termitomyces (Termitomyces albuminosas<Berk>heim), aforesaid chemical examination both can independently have been chemically examined; Also can entrust termite control research institution chemical examination, BeiJing, China city nutrient source institute or similarly department (mechanism) chemical examination that can also entrust the applicant in background technology, to mention, chemical examination finish and confirm qualified after; Earlier Odontotermes formosanus Shiraki population and the Odontotermes formosanus Shiraki main nest bacterial nursery that contains termitomyces are placed dewatered drying in the thermostatic drying chamber by weight 1: 15 proportion relation; The thermoregulation of thermostatic drying chamber is 100 ℃, and be 240min drying time, obtains Odontotermes formosanus Shiraki population stem body and the Odontotermes formosanus Shiraki main nest bacterial nursery stem body that contains termitomyces after oven dry finishes; Then with its pulverizing; Be about to Odontotermes formosanus Shiraki population stem body and put into the ball mill ball milling jointly with the Odontotermes formosanus Shiraki main nest bacterial nursery stem body that contains termitomyces, cross 90 mesh sieves behind the ball milling, sieve is got the dry powder body; The dry powder body radiation sterilization 185min that then uses cobalt 60 (Co60) that sieve is got; It is stirring and dissolving in the ethanol that dry powder body after will sterilizing again joins solvent, the dry powder body after the sterilization is mixed with ethanol equably, and under>=15 ℃ ambient temperature, leaving standstill 300-360min after the mixing; So that effective ingredient is dissolved in the ethanol fully; With the filter membrane of 10-20 μ m ceramic membrane filter for example, use foreign bodies such as removing earth, crude fibre, sandstone, obtain transparent solution; Pour transparent solution in distil container such as alembic distilling under reduced pressure; And reclaiming ethanol and utilize, specifically is to adopt vacuum pump to extract the air in distil container such as the alembic, and the pressure of evacuation is 0.0067MPa; In the process of distilling under reduced pressure; Place bath to be heated to 50-60 ℃ the alembic that fills transparent liquid, the solid that obtains is the fungus garden of Odontotermes formosanus compositions powder body that Odontotermes formosanus Shiraki population powder body and the Odontotermes formosanus Shiraki main nest bacterial nursery powder body that contains termitomyces mix, and is for use; The qualified notion of affirmation described in this step is meant: termite population really is the Odontotermes formosanus Shiraki population, and termite nest fungus comb is strictly the Odontotermes formosanus Shiraki main nest bacterial nursery that contains termitomyces;
B) take by weighing by steps A) the fungus garden of Odontotermes formosanus compositions powder body 10g that obtains; And it is joined throw in the container such as plastic containers or rustless steel container of deionized water that the purity that 20g (20ml) is arranged is 0.08 μ s/cm; Stir; Mixing time is 10min, obtains fungus garden of Odontotermes formosanus composition powder liquid solution, and is for use;
C) be the deionized water for stirring 5min that purity that collagen protein powder joins 20g (20ml) is similarly 0.08-0.12 μ s/cm with the 4.5g medical collagen, adding mass percent concentration again is that 27.5% antibacterial is hydrogen peroxide (H
2O
2) 7g stirs 30min then, carries out endotoxin inactivation by hydrogen peroxide and handles, preferred scheme can adopt TAL to detect whether reach the endotoxin inactivation requirement, obtains collagen solution, and is for use;
D) will be by step B) the fungus garden of Odontotermes formosanus composition powder liquid solution that obtains joins by step C) in the collagen solution that obtains; Stir; Concrete mixing time is 5min, adds 938.46g's again and purity still is the deionized water of 0.08-0.12 μ s/cm, and then stirs; Concrete mixing time is 5min, obtains mixed liquor;
E) ternary acid with SILVER REAGENT is that phosphoric acid 0.04g joins by step D) stir in the resulting mixed liquor; Concrete mixing time is 5min; By phosphoric acid the pH value of mixed liquor is adjusted into pH=5, after leaving standstill 40min, filters with 100 purpose nylon leaching nets; Filter the back fill, obtain 1000g hemorrhage finished product.
The mixing speed of the stirring that above-mentioned each step is related all is as the criterion with 60-100n/min.
Embodiment 2:
Only with steps A) in the Odontotermes formosanus Shiraki population change 1: 20 into the weight ratio that contains the Odontotermes formosanus Shiraki main nest bacterial nursery of termitomyces; With the thermoregulation of thermostatic drying chamber is 90 ℃; Change 280min drying time into; Grit number excessively behind the ball milling is 100 mesh sieves, and the time of radiation sterilization changes 235min into, and solvent is used water (promptly using water extraction) instead;
Only with step B) in the amount of fungus garden of Odontotermes formosanus compositions powder body change 11g into, and it is joined to throw the purity that 20g (20ml) is arranged be that the mixing time of the distilled water of 0.12 μ s/cm changes 9min into;
Only with step C) in the amount of collagen protein change 4g into; Deionized water changes the distilled water that purity is 0.12 μ s/cm into, and mixing time changes 6min into, and antibacterial is that the mass percent concentration of hydrogen peroxide changes 26% into; The amount of hydrogen peroxide changes 7.2g into, and the time of stirring then changes 32min into;
Only with step D) in mixing time change 6min into, change deionized water into distilled water that purity is 0.12 μ s/cm, the amount of distilled water is 937.765g, and then the time of stirring changes 6min into;
Only with step e) in the amount of phosphoric acid change 0.035g into, mixing time changes 6min into, pH value changes pH=5.1 into, time of repose changes 45min into, obtains 1000g hemorrhage finished product.All the other are all with the description to embodiment 1.
Embodiment 3:
With steps A) in the weight ratio of Odontotermes formosanus Shiraki population and the termite nest fungus comb of the black wing soil that contains termitomyces change 1: 18 into; Change the temperature of thermostatic drying chamber into 85 ℃; Change 290min drying time into, the grit number of crossing behind the ball milling changes 85 orders into, and the radiation sterilization time changes 200min into;
With step B) in the amount of fungus garden of Odontotermes formosanus compositions powder body change 12g into, change mixing time into 8min, the purity of deionized water changes 0.1 μ s/cm into;
With step C) in the amount of collagen protein change 5g into, mixing time changes 7min into, antibacterial is that the consumption of hydrogen peroxide changes 7.4g into, the time of stirring then changes 33min into;
With step D) in stir with so that time of stirring change 7min into, purity is that the amount of the deionized water of 0.1 μ s/cm changes 935.57g into;
With step e) in the amount of phosphoric acid change 0.03g into, mixing time changes 7min into, pH value changes pH=5.2 into, time of repose changes 50min into, obtains 1000g hemorrhage finished product.All the other are all with the description to embodiment 1.
Embodiment 4:
With steps A) in the temperature and time of constant temperature drying case change 95 ℃ and 285min respectively into;
With step B) in the amount of fungus garden of Odontotermes formosanus compositions powder body change 13g into, deionized water is used distilled water instead, mixing time changes 7min into;
With step C) in the amount of collagen protein change 5.5g into, deionized water changes distilled water into, antibacterial is hydrogen peroxide (H
2O
2) amount change 7.6g into, the mass percent concentration of hydrogen peroxide changes 28% into, mixing time and the time of stirring then change 8min and 34min respectively into;
With step D) in mixing time with so that mixing time all change 8min into, deionized water is used distilled water instead, the amount of distilled water changes 933.87g into;
With step e) in phosphoric acid change citric acid into, the amount of citric acid changes 0.025g into, mixing time changes 6.5min into, pH value changes pH=5.5 into, time of repose changes 55min into, obtains 1000g hemorrhage finished product.All the other are all with the description to embodiment 1.
Embodiment 5:
With step B) in the amount of fungus garden of Odontotermes formosanus compositions powder body change 15.5g into, mixing time changes 5min into;
With step C) in the amount of collagen protein change 6g into, mixing time changes 9min into, hydrogen peroxide (H
2O
2) amount change 9g into, mixing time changes 35min into then;
With step D) in mixing time with so that mixing time respectively change 9min into, the amount of deionized water changes 929.48g into;
With step e) in the amount of phosphoric acid change 0.02g into, mixing time changes 10min into, pH value changes pH=6 into, time of repose changes 60min into, obtains 1000g hemorrhage finished product.All the other are all with the description to embodiment 1.
It is primary raw material that the foregoing description adopts fungus garden of Odontotermes formosanus compositions powder body; The preparation hemorrhage; Can yet be regarded as is a kind of innovation, because Coptotermes formosanus Shtrari. is classified as one of worldwide five major pests (Ticks, glossina palpalis, mythimna separata, mosquito, Coptotermes formosanus Shtrari.) by UNESCO, the applicant develops Coptotermes formosanus Shtrari.; Change harmful to treasure, service is human.The saying that " Chinese medicine effects a permanent cure " just arranged since ancient times, fungus garden of Odontotermes formosanus are simply important in China's Chinese medicine system, are rich in each seed amino acid, linoleic acid, oleic acid, stearic acid and human body necessary macroelement calcium and trace element; Very high medical value is arranged; A large amount of zooperies prove that fungus garden of Odontotermes formosanus has tangible antiinflammatory, detumescence, analgesic activity, and the reduction glutamate pyruvate transaminase is arranged; The liver protecting is avoided the damage of poisonous substance, and promotes the repair function behind the hepatocyte acute injury.Clinically be used for human experimentation and also show, take the fungus garden of Odontotermes formosanus capsule to rheumatoid and rheumatic arthritis, senile osteoporosis is sick, insomnia and constipation and other diseases are all effective in cure, and effects such as the defying age of being good for the stomach and raise immunity are arranged.
Hemorrhage of the present invention is except can being applied in general hemostasis situation; Coagulation factor deficiency (hemophilia A or hemophilia B) and light moderate thrombocytopenia can also be applied to and, all satisfied haemostatic effect can be obtained through special circumstances such as aspirin or heparin therapies.This hemorrhage is prepared with natural biologic material, and it is good to have a haemostatic effect, and excellent antiinflammatory, pain relieving effect are arranged, and can not produce untoward reaction to the patient, and non-stimulated.
Claims (10)
1. hemorrhage, it is characterized in that comprising in the finished product liquor of every 1000g weight: fungus garden of Odontotermes formosanus compositions powder body 10-16g, collagen protein 4-6g, ternary acid 0.02-0.04g and antibacterial 7-9g, surplus is a water.
2. hemorrhage according to claim 1 is characterized in that described fungus garden of Odontotermes formosanus compositions powder body is the mixture of termite population powder body and termite nest fungus comb powder body.
3. hemorrhage according to claim 2, the weight ratio that it is characterized in that described termite population powder body and termite nest fungus comb powder body is 1: 15-20.
4. according to claim 1 or 2 or 3 described hemorrhages, it is characterized in that described Coptotermes formosanus Shtrari. is an Odontotermes formosanus Shiraki, described fungus garden is the fungus garden that contains termitomyces.
5. hemorrhage according to claim 1 is characterized in that described collagen protein is a medical collagen, and described antibacterial is that mass percent concentration is the hydrogen peroxide of 26-28%.
6. hemorrhage according to claim 1 is characterized in that described ternary acid is the phosphoric acid or the citric acid of SILVER REAGENT, and described water is that purity is deionized water or the distilled water of 0.08-0.12 μ s/cm.
7. the method for preparing of a hemorrhage as claimed in claim 1 is characterized in that may further comprise the steps:
A) produce fungus garden of Odontotermes formosanus compositions powder body; The termite nest fungus comb dewatered drying that contains termite population that will from Coptotermes formosanus Shtrari. ant road, dig out earlier, pulverize and sieve, sterilization then, reuse dissolution with solvents, filtration and distillation and reclaim solvent; Obtain fungus garden of Odontotermes formosanus compositions powder body, for use;
B) fungus garden of Odontotermes formosanus compositions powder body is added to the water stirs, obtain fungus garden of Odontotermes formosanus composition powder liquid solution;
C) collagen protein powder is added to the water stirs, add antibacterial again and behind 30-35min, stir then, obtain collagen solution;
D) fungus garden of Odontotermes formosanus composition powder liquid solution is joined in the collagen solution, stir, add entry again and then stir, obtain mixed liquor;
E) ternary acid joined in the mixed liquor stir, the pH value of mixed liquor is adjusted to 5-6, through leave standstill, filtration and fill, obtain the finished product hemorrhage.
8. the method for preparing of hemorrhage according to claim 7 is characterized in that steps A) described in the used device of dewatered drying be the constant temperature drying case, bake out temperature is 80-100 ℃; Drying time is 240-300min; The used device of described pulverizing is a ball mill, described sieving to crossing 100 mesh sieves, and described sterilization is for adopting the co-60 radiation sterilization; The time of radiation sterilization is 180-240min; Described solvent is water or ethanol, describedly is filtered into that to adopt the aperture be the membrane filtration of 10-20 μ m, and described distillation is distilling under reduced pressure.
9. the method for preparing of hemorrhage according to claim 7 is characterized in that step C) described in the collagen protein powder mixing time that stirs that is added to the water be 5-10min, the described mixing time that stirs then is 30-35min.
10. the method for preparing of hemorrhage according to claim 7 is characterized in that step D) described in stir with so that the mixing time that stirs respectively be 5-10min; Step e) mixing time that stirs described in is 5-10min, and the described time of leaving standstill is 40-60min, and described being filtered into adopted 100 purpose strainer filterings.
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| CN103635202B (en) * | 2011-04-27 | 2016-09-28 | 比奥马普公司 | Hemostatic composition |
| CN105617363A (en) * | 2016-02-29 | 2016-06-01 | 苏州市贝克生物科技有限公司 | Traditional Chinese medicine hemostatic powder and preparation method thereof |
| JP7039559B2 (en) | 2016-08-12 | 2022-03-22 | ビオムプ、フランス、エスアエス | Hemostatic flowable agent |
| EP3752211B1 (en) | 2018-02-15 | 2023-12-27 | Dilon Technologies Inc. | Multi-dimensional hemostatic product and method for producing the same |
| CN108310016A (en) * | 2018-05-07 | 2018-07-24 | 胡彬 | A kind of preparation method of external application cream for arthralgia |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1139553A (en) * | 1995-06-30 | 1997-01-08 | 中国林业科学研究院森林保护研究所 | Use of a pharmaceutical composition comprising a termite population and its host colony |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1139553A (en) * | 1995-06-30 | 1997-01-08 | 中国林业科学研究院森林保护研究所 | Use of a pharmaceutical composition comprising a termite population and its host colony |
Non-Patent Citations (1)
| Title |
|---|
| 李扬缜，等.《白蚁菌圃治疗痔疮出血124例》.《安徽中医临床杂志》.1998,第10卷(第5期),276-277. * |
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