CN105494269B

CN105494269B - A kind of feeding method of sugarcane two-spotted borer

Info

Publication number: CN105494269B
Application number: CN201511006336.XA
Authority: CN
Inventors: 魏吉利; 黄诚华; 潘雪红; 商显坤; 王伯辉
Original assignee: Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences
Current assignee: Guangxi Zhuang Nationality Autonomous Region Academy of Agricultural Sciences
Priority date: 2015-12-29
Filing date: 2015-12-29
Publication date: 2021-04-27
Anticipated expiration: 2035-12-29
Also published as: CN105494269A

Abstract

本发明公开了一种甘蔗二点螟的饲养方法，在养虫盒中加入玉米棒；将二点螟卵块放入第一培养皿中，然后将第一培养皿放入养虫盒中；待二点螟卵块在第一培养皿中初孵化成幼虫，保持供应玉米棒至幼虫化蛹；将蛹取出放至第二培养皿中保湿，并将第二培养皿放入养虫笼中，待蛹羽化；在养虫笼中放入甘蔗苗及蜂蜜水，待成虫于甘蔗苗叶上产卵；将附着有卵块的甘蔗苗叶剪下，放入第一培养皿中后，循环前述步骤，这种饲养方法成活率和繁殖率更高，用玉米饲养的世代数相比以往增加了1～2代，操作简单，成本低廉，因此可在室内饲养，可为虫害研究提供稳定充足的虫源。The invention discloses a feeding method for the sugarcane locust borer. The corn cob is added to the worm-raising box; the worm-bearing worm egg mass is put into a first culture dish, and then the first culture dish is put into the worm-raising box; In the first petri dish, the egg mass of the two-point borer was hatched into larvae, and the corn cob was kept supplied until the larvae pupated; the pupae were taken out and placed in the second petri dish to keep moisture, and the second petri dish was put into the worm cage. Pupae emerge; put sugarcane seedlings and honey water in the worm cage, and wait for adults to lay eggs on the sugarcane seedling leaves; cut off the sugarcane seedling leaves with egg masses attached, put them into the first petri dish, and cycle the aforementioned steps, This feeding method has higher survival rate and reproduction rate, and the number of generations raised by corn is increased by 1 to 2 generations compared with the past. The operation is simple and the cost is low. Therefore, it can be raised indoors, which can provide a stable and sufficient insect source for insect pest research. .

Description

Method for raising sugarcane dibbler

Technical Field

The invention relates to a method for raising Diatra nubilalis.

Background

The sugarcane dibotrys is an important stem-boring pest of sugarcane, is widely distributed in various sugarcane-planting countries, is distributed in various sugarcane areas in China, and harms millet, chyle, sorghum, corn and other crops besides sugarcane. The Diaphlus pyralis damages sugarcane by larvae, dead seedlings are formed in the seedling stage, the sugarcane is lack of seedlings and broken ridges, the stem borer nodes are caused in the growth period, the sugarcane yield is influenced, and the Diaphlus pyralis is a main stem borer pest on the sugarcane, so that the Diaphlus pyralis becomes one of main targets for preventing and controlling the sugarcane stem borers. However, no better method for breeding the sugarcane dibbler exists at present, and no sufficient insect source provides a basis for pest control.

The method for breeding the stem borers by using the sugarcane is introduced to be close to the field, the temperature, humidity and feed of the method are close to the field conditions, the feed is the sugarcane, but the detailed description of whether the feed is a sugarcane seedling or a sugarcane stem or other steps such as acquisition, preparation, replacement and the like of the feed are not introduced. The method also indicates that after three years of breeding, specific numbers and steps can be omitted for proving, the method also uses sugarcane seedlings and sugarcane stalks for comparative tests, the survival rate and the reproduction rate of the larvae of the Diatra nubilalis are lower than those of the Diatra nubilalis bred by corns, the generation number of the Diatra nubilalis bred by the corns is increased by 1-2 generations, more insect sources are provided, the cost is low, the operation is simple, and therefore, the Diatra nubilalis bred by the corns can be subcultured indoors.

Disclosure of Invention

Based on the above, there is a need for a method for raising the chilo suppressalis, which can be subcultured indoors, has high survival rate and reproduction rate, and is low in cost.

According to one aspect of the invention, the invention provides a method for raising sugarcane dibbler, which comprises the following steps:

adding corn cobs into the insect breeding box;

putting the egg blocks of the chilo suppressalis into a first culture dish, and then putting the first culture dish into an insect breeding box;

when the egg mass of the Diatra medinalis is primarily hatched into larvae in a first culture dish, keeping supplying the corn cobs until the larvae pupate;

taking the pupae out, putting the pupae into a second culture dish for moisturizing, and putting the second culture dish into an insect cage until the pupae eclosion;

putting sugarcane seedlings and honey water into the insect rearing cage, and allowing adults to lay eggs on sugarcane seedling leaves;

and cutting off the sugarcane seedlings with the attached egg blocks, putting the sugarcane seedlings into a first culture dish, and then circulating the steps.

In one embodiment, the corn cob is fresh corn cob, stored in a refrigerator at 4 deg.C, peeled off, and cut into pieces before being placed in the insect-keeping box.

In one embodiment, the concentration of the hydromel is 10%.

The method for breeding the chilo suppressalis has the advantages of higher survival rate and reproduction rate, 1-2 generations of generations increased compared with the conventional method for breeding the chilo suppressalis by using corns, simplicity in operation and low cost, so that the chilo suppressalis can be bred indoors, and a stable and sufficient insect source can be provided for insect pest research.

Detailed Description

To facilitate an understanding of the invention, the invention will now be described more fully with reference to the following specific examples. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, this embodiment is provided so that this disclosure will be thorough and complete.

The method for raising the chilo suppressalis of the embodiment comprises the following steps:

s1, adding corn cobs into the insect breeding box;

s2, placing the egg blocks of the Diatra medinalis into a first culture dish, and then placing the first culture dish into an insect box;

s3, hatching egg masses of the Diatra walker in a first culture dish to form larvae, and keeping supplying corn cobs until the larvae pupate;

s4, taking out the pupae, putting the pupae into a second culture dish for moisturizing, and putting the second culture dish into an insect cage until the pupae eclosion;

s5, placing sugarcane seedlings and honey water in an insect rearing cage, and allowing adults to lay eggs on sugarcane seedling leaves;

s6, cutting the sugarcane seedlings with the attached egg blocks, and placing the sugarcane seedlings into a first culture dish.

S7, the above steps S1 to S6 are repeated.

Specifically, the breeding method of the embodiment is carried out in a laboratory, the indoor temperature is kept at 25-28 ℃, and the relative humidity is 75-85%.

Specifically, the corn cobs used in this example are fresh, peeled and sweet corn cobs that are safe and non-toxic (no pesticide is sprayed and no pest is damaged), and are brought back to the laboratory to be stored in a refrigerator at 4 ℃ for later use, when used, the corn cobs can be taken out and peeled, and then cut into 5 cm-long sections with a knife.

Specifically, in step S1, fresh corn cobs are cut into segments and placed into sterilized insect boxes, and one insect box holds a segment of corn cobs.

Specifically, in step S2, the darkened egg masses of the chilo suppressalis are placed in first culture dishes with four layers of gauze, and four darkened egg masses of the chilo suppressalis are placed in one first culture dish.

Specifically, in step S2, a wet cotton ball is placed in the first culture dish for moisturizing.

Specifically, in step S3, the first culture dish is placed in a previously prepared insect box, and then the box cover is closed, and the first hatched larva after hatching automatically climbs into the corn cob for eating. And after all the larvae which are hatched into the corn cobs, taking out the dead leaves of the egg shells and the first culture dish from the larva breeding box, and only remaining the corn cobs of the larvae of the Diatra. Treat that the corn-on-cob is got by the larva and eats the back, the larva can climb out by oneself, prepare 4 insect feeding boxes of putting the corn-on-cob this moment, put into 4 insect feeding boxes that have the maize section with the brush gently with the larva respectively, roughly 30 larvae are put into to every insect feeding box, raise under same humiture condition. Raising until the larva climbs out by itself, replacing the larva raising box again, moving the larva into the larva raising box with fresh corn section, adjusting the corn section to 3cm in length at the moment, and starting pupation when the larva is fed to the end age.

Specifically, during pupation, most Diaochinia nubilalis can drill out corn cobs and pupate in a box, and in the pupation period, the pupae are taken out by a plastic small spoon when the occurrence of the pupae is observed every day; a small part of larvae are left in the corn for pupation, and the pupae can be taken out after pupation.

Specifically, in step S4, the pupae are placed in second culture dishes with four layers of gauze, 30 pupae are placed in each second culture dish, the wet cotton balls are placed in the second culture dishes for moisture preservation and are placed in insect cages, water is added to the wet cotton balls every day for moisture preservation, the second culture dishes are replaced every two days, and four pupae culture dishes can be placed in each insect cage for emergence.

Specifically, in step S5, a can bottle filled with sugarcane seedlings is placed in an insect cage with pupas, water is filled in the can bottle to keep the sugarcane seedlings fresh, adults mate in the cage after eclosion and lay eggs on sugarcane seedling leaves, a culture dish paved with four layers of gauze is placed in the insect cage, honey water with the concentration of 10% is added into the culture dish, wet cotton balls are placed in the culture dish for moisturizing, the adults are supplemented with nutrition, the honey water is added every day, and the culture dish is replaced every two days.

Specifically, in step S6, the egg pieces on the leaves of the sugarcane seedlings are cut off with leaves every day and cut into strips with a length of 8cm, the two ends of the leaves of the sugarcane seedlings are moisturized with wet cotton and placed into culture dishes paved with four layers of gauze, wet cotton balls are placed into the culture dishes, the culture dishes are placed into insect-raising boxes, four eggs can be placed into each culture dish, water is added into the wet cotton balls every day for moisturizing, so that the leaves of the sugarcane seedlings with the egg pieces keep tender green and are kept black. After the larvae are hatched, the steps from S1 to S6 are repeated, and the operation of the embodiment is circulated, so that the larvae can be subcultured.

Identification of feeding effect: in this embodiment, after 12 generations of indoor continuous breeding, the main life parameters of each insect state are measured in the 1 st, 4 th, 7 th and 10 th generations, specifically as follows:

vital parameters	Generation 1	Generation 4	7 th generation	Generation 10
					Hatching rate/%	93.26	91.48	88.10	99.45
Survival rate of larvae/%)	42.72	55.00	50.22	49.53
					Eclosion Rate/%	87.72	92.42	88.59	81.00
Egg laying amount per granule for single female	29.20	49.51	47.58	37.40

The results show that the difference of life parameters among generations is small, the multiplication times are high, and the method can completely meet the requirement of indoor subculture feeding of the Diatra sacchari and can obtain a large number of test insects with good growth and development in a short period.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims

1. the raising method of a kind of sugarcane two-spotted borer, is characterized in that, comprises the steps: in worm raising box, add corn cob;

Putting the egg mass of A. chinensis into the first petri dish, and then putting the first petri dish into the worm-keeping box; when the egg mass of P. chinensis is initially hatched into larvae in the first petri dish, maintaining the supply of the cob until the larvae pupate;

The pupa is taken out and placed in the second petri dish to keep moisture, and the second petri dish is put into the insect cage, until the pupa emerges;

Put sugarcane seedlings and honey water in the worm cage, and wait for adults to lay eggs on the sugarcane seedling leaves;

Cut off the sugarcane seedling leaves with egg masses attached, put them into the first petri dish, and repeat the above steps; during the feeding process, keep the indoor temperature at 25-28° C. and the relative humidity at 75-85%.

2. the raising method of a kind of sugarcane two-spotted borer according to claim 1, is characterized in that, described corn cob is fresh corn cob, and is stored in 4 ℃ of refrigerators, and described corn cob is peeled during use, and is placed on the cob. The corn cobs were cut into sections before being put into the insect box.

3. the raising method of a kind of sugarcane two-spotted borer according to claim 1, is characterized in that, the concentration of described honey water is 10%.