CN105165734B - A kind of method of Adoxophyes spp cocoon honeybee biological control - Google Patents

Abstract

The invention discloses a method for indoor breeding of the brown-banded moth wasps. At 25° C. of temperature, light: under the condition of darkness=14h:10h, put into the insect cage with sleeves of 55cm * 55cm * 55cm by the brown scalloped wasp, feed with 15% honey water, every 3 Every day, take out the feed with worms from the breeding tray with 5-6 instar rice moth larvae and spread it on the bottom of the plastic box, so that the height of the feed with worms is about 1.5cm, and put the plastic box without a cover into the parasitic bee insect cage Among them, the parasitic wasps take the rice moth in the feed as their host. After 3 days, the plastic box was taken out, and the lid was covered. After 12 days, the next generation of parasitoids began to emerge, and the emergence time continued for more than 7 days. The invention has the advantages of simple operation and high efficiency, and is easy to realize the large-scale reproduction of the coronis.

Description

A kind of method of large-scale reproduction of the brown banded moth Braconis

technical field

The invention relates to the field of biological control of agricultural and forestry pests, in particular to a method for the large-scale propagation of the brown-banded moth wasps.

Background technique

Coconut weaver moth Opisina arenosella Walker is an important pest on palm plants, mainly damaging palm plants such as coconut, palmetto, Middle East date palm, areca nut, and king palm. In August 2013, Wanning City, Hainan Province first discovered the damage caused by the coconut weaver moth, and in December 2013, it was found to be infested by the insect in Guangxi. invade our country. This pest has occurred in India, Thailand, Indonesia and other Southeast Asian countries and regions, and has caused significant economic losses to local palm plants. After it invaded our country, it also attracted the attention of relevant departments. In 2014, it was included in the list of invasive alien pests by the State Forestry Administration.

For taller palm plants such as coconuts, it is difficult to implement chemical control, and the larvae form moth tunnels intertwined with feces and silk on the back of the leaves, sneaking and harming in the moth tunnels, and the chemical control effect is poor. At the same time, chemical control pollutes the environment, which can easily lead to drug resistance of the coconut weaver moth. Therefore, according to the classical biological control theory, it is an effective way to use natural enemies to control the weaver moth. Bracon adoxophyesi Mimanikawa is an ectoparasitic wasp of middle and advanced larvae of the coconut weaver moth. It lays eggs outside the larvae of the coconut weaver moth, and first paralyzes the host when laying eggs. The bee larvae live outside the body of the host larvae, and when they mature, they cocoon and pupate near the body of the host. Poly parasitism. The results of the research show that the adult period of the brown scalloped moth is about 60 days, and it has a strong ability to paralyze the host. One female wasp can paralyze about 6 senior larvae of the coconut moth in one day, and the total egg production of each female is up to 386- 485 grains, short development cycle (25°C, about 12 days to reproduce 1 generation), very easy to reproduce. However, due to the fact that the larvae of its host coconut moth are easily infected with germs and die during the feeding process, and at the same time, the larvae are sneaking under the moth tunnel intertwined with insect feces and silk, the process of raising and changing leaves is heavy, labor-intensive and time-consuming, and it is difficult to achieve large-scale breeding. There are certain difficulties in using it as a host to raise a large number of B.

Contents of the invention

(1) Technical problems to be solved

In order to overcome the deficiencies of the existing technology, a method for the large-scale reproduction of Braconis sativa is proposed, using rice moth larvae as the breeding host instead of the natural host coconut moth, which has good parasitic effect and high reproductive efficiency.

(2) Technical solution

The present invention is realized through the following technical solutions: the present invention proposes a method for the large-scale propagation of Braconidia spp., which is characterized in that the method comprises the following steps:

(1) Host reproduction: spread a layer of feed with a thickness of 1.5 cm in the feed tray. The feed ingredients include 70 parts of corn flour, 20 parts of wheat bran, 7 parts of white sugar, and 3 parts of yeast. Sprinkle rice moth eggs, and then use a sieve to sprinkle a layer of feed that just covers the eggs. After the hatched larvae eat densely packed small holes, feed once every 3 days. The amount of input is the food intake of larvae for 3 days. Take 5- The 6th instar rice moth larvae are used as breeding hosts;

(2) Collection of bee species: During the peak period of damage caused by the cocoon moth, the larvae of the cocoon moth parasitized by the cocoon moth were collected back to the room to collect the cocoons of the cocoons of the cocoons. Bee is put into the insect cage with sleeve, and the insect cage specification is 55cm * 55cm * 55cm, temperature 25 ℃, light: under the condition of dark=14h: 10h, be 15% honey water feeding with mass percentage, standby;

(3) Brown banded moth wasp multiplication: take out the feed with worms from the rearing tray with 5-6 instar rice moth larvae, spread out the feed with worms, remove worm droppings and dropable worms with a 20-mesh sieve Feed, then tiled at the bottom of the plastic box, so that the worm feed height is about 1.5cm, this plastic box without a cover is inserted in the insect cage of the brown banded moth described in step (2), and the brown banded moth The wasp drilled into the nest of the rice moth larvae to parasitize the rice moth larvae. After 3 days, the plastic box was taken out and the lid was closed. After 12 days, the next generation of parasitoids began to emerge. The eclosion time lasted for more than 7 days. Feed for 15% honey water;

(4) Preservation of the bee species: in the step (3), the ratio of the number of the larvae of the brown banded moth and the host rice moth larvae is 1:1, and the time of receiving the bee is 1 day, and the 4-day-old cocoons of the brown banded hornet Place them in a 15°C incubator for 30 days to preserve the bee species.

(5) Rejuvenation of the bee species: after the artificial propagation of the brown banded moth wasp in step (3) for 5-6 generations, the coconut trees harmed by the coconut weaver moth are selected outdoors, and the bees are released. The coconut weaver moth that parasitizes the wasps is brought back indoors, and the wasps emerge into adults, which is the rejuvenated bee species in the wild.

A more preferred scheme, the rice moth larvae reared to 5-6 instars in the step (1) are transferred to an incubator at 15° C. and can be stored for 100 days.

A more preferred scheme, the lid of the described step (3) is provided with an opening with a length of 10 cm and a width of 3 cm, and the opening is glued with a 100-order nylon mesh.

In a more preferred scheme, in the step (3), the plastic box is replenished with worm-carrying feed every 3 days, and the ratio of the number of the larvae to the larvae of the larvae and the larvae is 1:3.

(3) Beneficial effects

The present invention has the following beneficial effects:

The method for large-scale reproduction of a brown scalloped wasp mentioned in the present invention is based on the fact that the brown stippled wasp can parasitize the coconut weaver moth larvae that sneak and harm under the moth tunnels intertwined with insect feces and silk, and like to drill into the gaps , can drill into the habit of parasitic larvae in the nest made of rice moth larvae from the mouth of the nest, and put the rice moth larvae in the nest and feed directly into the beekeeping cage for the parasitism of the brown banded moth larvae, and the parasitic effect very good. In the process of receiving bees, it is only necessary to take out the rice moth feed with insects, spread it out (to create more gaps, so that the brown scalloped moth can drill into the gaps to search for the host), pass through a 20-mesh sieve, and remove the insect droppings and residues. The feed that falls is then tiled at the bottom of the plastic box, making the feed with worms highly about 1.5cm (the height of the nest being constructed by the rice moth larvae is about 1.5cm, making the rice moth nest roughly a single-layer distribution). The process of receiving bees in the invention is simple to operate, saves labor and time-consuming steps of taking out rice moth larvae from the silk-interwoven feed for receiving bees, and truly realizes an efficient process of multiplying bees.

detailed description

In order to make the object, technical solution and advantages of the invention clearer, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

A method for large-scale reproduction of the brown scalloped wasp, the method comprising the following steps:

1. Host reproduction:

Spread a layer of feed with a thickness of 1.5 cm in the feed pan. The feed ingredients include 70 parts of corn flour, 20 parts of wheat bran, 7 parts of white granulated sugar, and 3 parts of yeast in parts by weight. When preparing the feed, mix corn flour and wheat bran Put it into a drying box, keep the temperature at 80°C and heat for 4 hours to kill the germs, pests, mites and their eggs in the feed, sprinkle the rice moth eggs evenly on the feed, and then use a sieve to sprinkle a layer of feed that just covers the eggs , After the hatched larvae eat out of the densely packed small holes, they feed once every 3 days, and the input amount is the food intake of the larvae for 3 days.

The brown banded moth wasp can parasitize 3-7 instar rice moth larvae. Introduce a pair of 10-day-old male and female adults of Braconia japonica into the finger tube. According to the weight of rice moth larvae, put 21 3rd-instar larvae, 18 4th-instar larvae, 6 5th-instar larvae, and 6-instar larvae. 4 larvae and 3 7th-instar larvae were removed. After 3 days, the adult parasitoid wasps were removed. Each treatment was repeated 10 times. The parasitism rate, lethality rate, and number of offspring bees of the wasp to larvae of different ages were recorded. The results are shown in Table 1:

Table 1 The parasitic ability of Braconis spp. to larvae of different ages

It can be seen from Table 1 that the paralysis and lethality rate of the 3-7 instar rice moth larvae to the 3-7 age rice moth larvae of the brown banded moth wasp is 100%. , the number of progeny bees produced by the 7th instar larvae as hosts (26±2 heads) was slightly more than the number of progeny bees produced by the progeny with 5-6 instar larvae as hosts (20±5 heads, 21±1 heads), However, if the 7th instar mature larvae are used as the host, some rice moths will have developed into prepupa and pupae in the feed containing 7th instar mature larvae due to the uneven development of rice moths. The parasitic wasps will emerge into rice moth adults, and if the rice moth escapes to the outside, it will cause harm to stored grain. Therefore, the 5-6 instar rice moth larvae should be used as hosts to carry out a large number of breeding of Braconis spp., and this process begins to raise about 40 days from the rice moth eggs at 25°C.

The 5-6 instar rice moth larvae are suitable intermediate hosts for large-scale propagation of the brown sash moth. Part of the rice moth larvae died), and the larvae can be stored for up to 100 days in an incubator at 15°C, which significantly improves the effective utilization rate of the rice moth larvae and meets the needs of the large-scale reproduction of the brown scalloped moth for the host insect source .

2. Collection of bee species:

During the peak period of damage caused by the coconut moth, the larvae of the coconut moth parasitized by the brown moth were collected and brought back to the room to collect the brown moth as a source of bee seeds. In the insect cage, the size of the insect cage is 55cm * 55cm * 55cm, the temperature is 25° C., light: under the condition of darkness=14h: 10h, it is 15% honey water to feed with the mass percentage, for subsequent use.

3. The increase in the number of brown-banded moths:

Take out the feed with worms from the breeding tray with 5-6 instar rice moth larvae, spread out the feed with worms, remove worm excrement and dropable feed with a 20 mesh sieve, and then spread it flat on the bottom of the plastic box to make the feed with worms spread out. The height of the worm feed is about 1.5cm. Put this plastic box without a cover into the insect cage of the brown-banded moth wasp described in step 2, and the brown-banded moth wasp drills into the nest of the rice moth larvae to parasitize the rice moth larvae After 3 days, take out the plastic box and cover the box lid. There is an opening with a length of 10 cm and a width of 3 cm on the box cover. The opening is glued with a 100-mesh nylon mesh for ventilation. For more than 7 days, the braconids were fed with 15% honey water after emergence.

4. Preservation of bee species:

In the step 3, the ratio of the number of the cocoons of the brown banded moth and the host rice moth larvae is 1: 1, and the bee receiving time is shortened to 1 day, and the 4-day-old cocoon of the brown banded cocoon is placed in a 15° C. incubator for 30 days, Used to preserve bee species.

5. Rejuvenation of bee species:

In step 3, after 5-6 generations of the artificial propagation of the brown scalloped wasp, the coconut trees harmed by the cocoon moth are selected outdoors, and the bees are released. After 10 days, the coconut weaver moth parasitized by the cocoon cocoon is collected in the wild , Take it back to the room, and the bee emerges into an adult, which is the rejuvenated bee species in the wild.

The field collection is to collect the larvae of the cocoa moth parasitized by the brown banded moth Braconis in the field, and cultivate them until they develop into adults.

6. Release in the wild:

The plastic box for the breeding of the brown scalloped wasp is hung on the tree through the wire, and the wire is coated with Vaseline. The parasitic wasps in the plastic box emerged continuously from the rice moth feed, and the emergence time lasted about 7 days. In order to ensure the control effect and prevent the influence of unfavorable factors in nature on the release of braconids, the method of increasing the amount of bees released is adopted. According to the occurrence of coconut moths, the amount of bees released is determined according to the ratio of insects and bees to 1:3, and the time interval for releasing bees for 7 days. Since the brown banded moth only parasitizes the middle-aged and advanced coconut moth larvae, it takes about 26 days for the coconut moth to develop from the newly hatched larvae to the middle-aged larvae, so the brown banded moth should be released 4 times in a row. , can realize the continuous control of the larvae of the coconut weaver moth, and reduce or eliminate the use of pesticides to achieve the purpose of preventing and controlling the coconut weaver moth.

Claims (5)

1. a kind of method of Adoxophyes spp cocoon honeybee biological control, it is characterised in that this method comprises the following steps：

(1) host breeds：The thick feeds of one layer of 1.5cm are spread in feed dish, feed ingredient is calculated by weight includes corn 70 parts of powder, 20 parts of wheat bran, 7 parts of white granulated sugar, 3 parts of yeast, it is uniform on feed to be sprinkled with rice moth egg, then just covered with sieving free-and-easy one layer The feed of lid ovum grain, the larva after hatching eats out every 3 days after thickly dotted aperture and throws a defective material, and input amount is larva 3 days Appetite, take 5-6 age rice moth larvas as host egg；

(2) honeybee kind is gathered：Moth is knitted in coconut to cause harm peak period, is gathered back moth larvae is knitted by the parasitic coconut of Adoxophyes spp cocoon honeybee Adoxophyes spp cocoon honeybee is put into the insect cage with sleeve by indoor collection Adoxophyes spp cocoon honeybee as honeybee kind introduces a collection, insect cage rule Lattice are 55cm × 55cm × 55cm, 25 DEG C of temperature, illumination: under the conditions of dark=14h: 10h, with the honeybee that mass percent is 15% Mulse is fed, standby；

(3) Adoxophyes spp cocoon honeybee increases numerous：Band worm feed is taken out from the rearing tray for giving birth to 5-6 age rice moth larvas, makes band worm feed Scatter, remove frass and the feed that can drop with 20 mesh sieves, be then laid in plastic casing bottom, make the band worm feed level be about 1.5cm, this plastic casing not with cover is inserted in step (2) the Adoxophyes spp cocoon honeybee insect cage, Adoxophyes spp cocoon honeybee pierces rice The cell endoparasitism rice moth larva of moth larvae, plastic casing is taken out, cover lid after 3 days, and parasitic wasp of future generation starts after 12 days Sprout wings, emergence time continues more than 7 days, after cocoon bee eclosion, fed with mass percent for 15% hydromel；

(4) honeybee kind rejuvenation：Adoxophyes spp cocoon honeybee manually increased after numerous 5-6 generations in step (3), and outdoor selection is knitted moth harm by coconut Honeybee is put in coco, progress, after 10 days, and moth is knitted in collection field by the parasitic coconut of Adoxophyes spp cocoon honeybee, brings back interior, bee eclosion is The honeybee kind of adult, as field rejuvenation.

2. a kind of method of Adoxophyes spp cocoon honeybee biological control according to claim 1, it is characterised in that：The step (1) raising to the rice moth larva in 5-6 ages is transferred to 15 DEG C of incubators and preserved 100 days in.

3. a kind of method of Adoxophyes spp cocoon honeybee biological control according to claim 1, it is characterised in that：The step (3) Adoxophyes spp cocoon honeybee and rice moth larva quantity ratio are 1: 1 in, connect the honeybee time and shorten to 1 day, by the age in days of Adoxophyes spp cocoon honeybee 4 Cocoon is placed in 15 DEG C of incubators and refrigerated 30 days, to preserve honeybee kind.

4. a kind of method of Adoxophyes spp cocoon honeybee biological control according to claim 1, it is characterised in that：The step (3) lid described in is provided with the wide 3cm of long 10cm opening, and opening is stained with 100 mesh nylon wires.

5. a kind of method of Adoxophyes spp cocoon honeybee biological control according to claim 1, it is characterised in that：The step (3) every 3 days supplement band worm feeds in plastic casing, Adoxophyes spp cocoon honeybee is 1: 3 with rice moth larva quantity ratio.