CN107182948A - Greater wax moth pilot scale culture new method - Google Patents

Abstract

Greater wax moth has broad application prospects in the fields of medicine, agriculture and pharmacy. The invention provides a new indoor large-scale breeding method for the greater wax moth. Put the cocooned honeycomb paper into the breeding box, and after the wax moth breaks the cocoon, it mates in the breeding box, and then the female moth lays eggs in the origami seam. After two days, all moths were killed and removed, and the larvae were allowed to grow naturally. After raising for one month, divide them into two boxes according to the size of the worms, and then raise them to the last instar larvae with artificial feed. Compared with the prior art, the improvement of the present invention lies in that the laying of eggs by the greater wax moth is actually carried out under the condition of the activities of bees, and the laying eggs are many and concentrated. Only two days before the collection, the difference in age of eggs was small. Raising in two stages is beneficial for individual size and density control and disinfection of rearing boxes.

Description

A New Method of Large-scale Culture of Melonella mellonella

technical field

The invention belongs to the insect breeding technology, and in particular relates to a new method for large-scale indoor breeding of the wax moth moth through natural honeycomb and artificial compound feed.

Background technique

The wax moth belongs to the order Lepidoptera, the moth family, the wax moth subfamily, and the wax moth is an insect. Under natural conditions, the greater wax moth mainly harms bee colonies and destroys the honeycomb, and is a major pest in beekeeping. In recent years, it has been gradually used in high-quality bait processing, experimental insects, multiplication of natural enemy insects, and development of biological pesticides. Greater wax moth has broad application prospects in the fields of medicine, agriculture and pharmacy. At present, there are few enterprises producing the wax moth in my country, and the scale is small. The main reason is that the spawning conditions of the wax moth are harsh, and the spawning is relatively hidden, so it is difficult to obtain a large number of eggs. Secondly, C. wax moth has a unique feeding behavior. It often spins silk to wrap itself and the spleen together. It is difficult to separate the spleen from C. mellonella. It is difficult to clean the breeding environment, which is not conducive to disease control. Thirdly, different ages of the greater wax moth are reared in the same box and bite each other, which seriously affects the output of adults. Currently, in the production of C. mellonella in my country, the oviposition technique mainly involves enclosing adults of C. mellonella in a small closed space, usually in a small box, and using origami to collect eggs. In this case, due to the narrow living space of adults, most of the male and female moths are close relatives, so the female moths lay fewer eggs, and the quality of the next generation is seriously degraded. In addition, due to the long egg-laying cycle of the female moth, the ages of the collected eggs are different, and the ages of the hatched larvae are also inconsistent, resulting in different sizes of the wax moth. Reasons such as above seriously limit the large-scale production of wax moth.

Contents of the invention

In order to overcome the above-mentioned technical deficiencies, the present invention provides a new method for large-scale indoor breeding of the wax moth. The present invention separates the male and female of the wax moth at the pupal stage, puts the male and female moths in a feeding box at a ratio of 1:2, and puts origami and natural nests on the pupae before they emerge. After laying eggs, lay a layer of artificial feed on the bottom of the box . After the eggs hatch, artificial feed is used for feeding. The technical scheme adopted in the present invention is: first, put the last instar larvae of the greater wax moth into the honeycomb paper to cocoon themselves; then put the cocooned honeycomb paper into the breeding box, put the natural honeycomb at the bottom of the box, and put the box in the honeybee Beside the box, the activity sound of bees is used to induce the smooth progress of the natural breeding behavior of the greater wax moth. After the wax moth breaks its cocoon, it mates in the breeding box, and then the female moth lays eggs in the origami seam. Two days later, all the moths were killed and removed, the bottom of the box was covered with artificial feed, and they were placed in the greenhouse to hatch, allowing the larvae to grow naturally. Finally, after the feed is eaten, the insect excrement is screened out, divided into two boxes according to the size of the insect body, and artificial feed is used to raise the last instar larvae. Compared with the prior art, the improvement of the present invention lies in that the laying eggs of the greater wax moth is actually realized under the imitation ecological conditions, and the laying eggs are many and concentrated. Only two days before the collection, the difference in age of eggs was small. Raising in two stages is beneficial for individual size and density control and disinfection of rearing boxes.

detailed description:

Listed examples below, the present invention is further described:

Example 1: Put the last instar larvae of the greater wax moth into the honeycomb paper to cocoon themselves; use a stereoscope to separate the male and female, prepare a plastic breeding box with a length, width and height of 40*50*30cm, and seal the box with an automatic With ventilation holes. Choose female cocoon 20, male cocoon 10 and put into raising box, put natural honeycomb at the bottom of the box, place folding fan paper at the bottom of the box, box is placed on the side of the bee box, and utilizes the active sound of bee to induce the smooth progress of the natural breeding behavior of the wax moth. After the wax moth breaks its cocoon, it mates in the breeding box, and then the female moth lays eggs in the origami seam. After two days, all moths were put to death and removed, and the artificial feed was covered at the bottom of the box. The artificial feed formula honeycomb 10%, honey 10%, flour 50%, corn flour 20%, beeswax 5%, yeast powder 5%. Put them in the greenhouse to hatch and let the larvae grow naturally. Finally, after the feed pre-stored at the bottom of the box is eaten, the insect droppings are screened out, divided into two boxes according to the size of the insect body, and artificial feed is used to raise the last instar larvae. In the end, 5,000 pieces of the late stage wax moth were obtained.

Claims (7)

1. The present invention provides a new method for laying eggs under ecologically imitative conditions and cultivating the wax moth with artificial feed. 2. according to claim 1, lay eggs under a kind of imitation ecology condition, it is characterized in that a box bee colony is placed in the spawning room, utilize the activity of bee colony to induce the greater wax moth to lay eggs. 3. According to claim 2, it is characterized in that the male and female are separated by a stereoscope at the pupal stage, and the male and female are paired at a ratio of 1:2. 4. According to claim 1, the new method for cultivating wax moth by artificial feed is characterized in that artificial feed formula honeycomb 10%, honey 10%, flour 50%, corn flour 20%, beeswax 5%, yeast powder 5%. 5. According to claim 1, the new method for cultivating the greater wax moth with artificial feed is characterized in that the eggs of the first two days are collected. 6. According to claim 1, the new method for cultivating the greater wax moth with artificial feed is characterized in that the artificial feed is directly placed at the bottom of the box, and the hatching allows the larvae of the greater wax moth to grow directly on the artificial feed. 7. According to claim 6, the wax moth grows on the artificial feed, which is characterized in that after one month of feeding, it is reared in boxes according to the size of the insect body.