CN102017929A - Method for artificially feeding ladybirds or lacewings by using lepidoptera insect larvae - Google Patents
Method for artificially feeding ladybirds or lacewings by using lepidoptera insect larvae Download PDFInfo
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- CN102017929A CN102017929A CN2009100924899A CN200910092489A CN102017929A CN 102017929 A CN102017929 A CN 102017929A CN 2009100924899 A CN2009100924899 A CN 2009100924899A CN 200910092489 A CN200910092489 A CN 200910092489A CN 102017929 A CN102017929 A CN 102017929A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
本发明了提供了一种人工饲养瓢虫和/或草蛉的方法，该方法包括以下步骤：(1)鳞翅目昆虫成虫产卵后孵化出鳞翅目昆虫幼虫，利用饲料饲喂鳞翅目昆虫幼虫；(2)以鳞翅目昆虫幼虫为饲料饲喂瓢虫幼虫和/或草蛉幼虫获得瓢虫成虫和/或草蛉成虫，所述草蛉选自大草蛉和日本通草蛉中的一种；(3)以鳞翅目昆虫幼虫为饲料饲喂瓢虫成虫和/或大草蛉成虫，而日本通草蛉的成虫则以蜂蜜和啤酒干酵母粉饲喂。与传统技术相比，本发明的方法以鳞翅目昆虫幼虫为饲料大规模饲养瓢虫、草蛉，代替了传统的以植物培养的蚜虫来饲养天敌昆虫瓢虫、草蛉的做法，该方法更加省时省力、简便易行。The present invention provides a method for artificially feeding ladybugs and/or lacewings, the method comprising the following steps: (1) Lepidoptera insect larvae are hatched after the adult lepidoptera insects lay eggs, and the lepidoptera insect larvae are fed with feed (2) feed ladybug larvae and/or lacewing larvae with Lepidoptera insect larvae to obtain ladybug adults and/or lacewing adults, and the lacewings are selected from the group consisting of Lacewing lacewing and Lacewing japonica (3) adult ladybugs and/or adult lacewings were fed on lepidopteran larvae, while adults of lacewings were fed on honey and brewer's dry yeast powder. Compared with the traditional technology, the method of the present invention uses the Lepidoptera insect larvae as feed to raise ladybugs and lacewings on a large scale, replacing the traditional method of raising natural enemy insects ladybugs and lacewings with plant-cultivated aphids. More time-saving and labor-saving, simple and easy to operate.
Description
Technical field
The present invention relates to extermination of disease and insect pest field.The present invention relates to a kind of lepidopterous larvae that adopts is the method that feed is raised ladybug or Chrysopa.
Background technology
Ladybug and Chrysopa are important predator insects, be mainly used in anti-eliminate aphis, mite class, scale insect, aleyrodid etc. relatively are difficult to the agriculture and forestry injurious insect prevented and treated.At present, the heavy industrialization breeding technology of ladybug or Chrysopa is unusual shortcoming also, still is in the very elementary stage, and it is immature that its key reason is to prepare the technology of artificial feed.And adopt natural feed such as aphid to carry out raising need plantation plant, and on plant, inoculate aphid, treat that aphid breeds certain density after, offer the predation of ladybug or Chrysopa again.Like this, feed and cost of labor are all very high, and need the plantation and the growing space of bigger aphid host plant.In addition, killing each other etc. of ladybug, Chrysopa also limited the large-scale industrialized production of ladybug and Chrysopa, becomes the limiting factor that a large amount of natural enemy insects are provided with low cost.
Summary of the invention
Therefore, the object of the present invention is to provide a kind of extensive artificial feeding ladybug simple and easy to do, with low cost and/or the method for Chrysopa; And the method for using it for the biological control of agriculture and forestry injurious insect.
On the one hand, the invention provides the method for a kind of artificial feeding ladybug and/or Chrysopa, for example, the method for a kind of artificial feeding ladybug or chrysopa septempunctata (Chrysopa septempunctata), this method may further comprise the steps:
(1) hatches the lepidopterous insects larva after the lepidopterous insects adult lays eggs, adopt the feed lepidopterous insects larva of feeding, this feed is counted by weight and is comprised: 200 parts in 10 parts of casein, 20 parts in wheat germ powder, 20 parts of tomatoes, 3 parts of potassium sorbates, 0.2 part of vitamin C, 0.7 part of rapeseed oil, 2 parts in agar and water;
(2) obtain grandis adults or chrysopa septempunctata adult with feed ladybug larva or chrysopa septempunctata larva of lepidopterous insects larva; And
(3) with lepidopterous insects larva feed grandis adults or chrysopa septempunctata adult, after laying eggs, adult hatches ladybug larva or chrysopa septempunctata larva.
Preferably, described step (3) may further comprise the steps:
I) under 25～27 ℃, relative moisture are 60～80% condition, with 2 age feed grandis adults or chrysopa septempunctata adult of lepidopterous insects larva, treat that adult lays eggs after 10～20 days;
II) collect egg hatch and go out ladybug larva or chrysopa septempunctata larva after, be to feed with the lepidopterous insects larva that just incubates under 60～80% the condition at 25～27 ℃, relative moisture with it; And
III) 3 or 4 age the ladybug larva or the chrysopa septempunctata larva with 2 age the lepidopterous insects larva feed.
Again for example, the method for a kind of artificial feeding expert on Japan Chrysopa (Chrysopa nipponensis), this method may further comprise the steps:
1) hatches the lepidopterous insects larva after the lepidopterous insects adult lays eggs, adopt the feed lepidopterous insects larva of feeding, this feed is counted by weight and is comprised: 200 parts in 10 parts of casein, 20 parts in wheat germ powder, 20 parts of tomatoes, 3 parts of potassium sorbates, 0.2 part of vitamin C, 0.7 part of rapeseed oil, 2 parts in agar and water;
2) obtain expert on Japan Chrysopa adult with the lepidopterous insects larva expert on Japan Chrysopa larva of feeding; And
3) with honey and the beer dried yeast powder expert on Japan Chrysopa adult of feeding, after laying eggs, adult hatches expert on Japan Chrysopa larva.
Preferably, described step 3) may further comprise the steps:
I) under 25～27 ℃, relative moisture are 60～80% condition,, treat that adult lays eggs after 10～20 days with honey and the beer dried yeast powder expert on Japan Chrysopa adult of feeding;
After ii) collecting egg hatch and going out expert on Japan Chrysopa larva, be to raise under 60～80% the condition to feed at 25～27 ℃, relative moisture with the lepidopterous insects larva that just incubates with it; And
Iii) 3 or 4 age expert on Japan Chrysopa larva with 2 age the lepidopterous insects larva feed.
Preferably, in described step (1), with feed and the hydromel lepidopterous insects adult of feeding, this feed is counted by weight and is comprised: 200 parts in 10 parts of casein, 20 parts in wheat germ powder, 20 parts of tomatoes, 3 parts of potassium sorbates, 0.2 part of vitamin C, 0.7 part of rapeseed oil, 2 parts in agar and water; The volumetric concentration of hydromel is 5～20%, is preferably 10%.
Preferably, in described step (2), the ladybug larva that just incubates, chrysopa septempunctata larva or expert on Japan Chrysopa larva adopt the lepidopterous insects larva that just incubates to feed, 3 or 4 age ladybug larva, chrysopa septempunctata larva or expert on Japan Chrysopa larva adopt 2 age the lepidopterous insects larva feed.
Preferably, described lepidopterous insects is a beet armyworm.
Further preferably, said method comprising the steps of:
A. the raising of lepidopterous insects
Adopt the feed lepidopterous larvae of feeding, and adopt this feed and the hydromel lepidoptera imago of feeding, treat to hatch larva after this adult lays eggs, adopt this feed larva of feeding once more, grow up to adult after pupating, this feed is counted by weight and is comprised: 200 parts in 10 parts of casein, 20 parts in wheat germ powder, 20 parts of tomatoes, 3 parts of potassium sorbates, 0.2 part of vitamin C, 0.7 part of rapeseed oil, 2 parts in agar and water; The volumetric concentration of described hydromel is 5～20%, is preferably 10%.
B. the raising of ladybug, chrysopa septempunctata or expert on Japan Chrysopa
A. imago breeding: at 25～27 ℃, under 60～80% the relative moisture condition, in dependent insect cage, raise adult, the adult of ladybug or chrysopa septempunctata add every day 2 age lepidopterous larvae, with the feed adult of expert on Japan Chrysopa of honey and beer dried yeast powder, treat to lay eggs after 10～20 days;
B. the collection of ovum and preservation: adopt egg holder or paper slip to put into the adult dependent insect cage, adult lays eggs on the groove or paper slip of egg holder, changes egg holder or paper slip, collects ovum and is stored in them in 10～12 ℃ of incubators or directly 25～27 ℃ of hatchings;
C. the raising of larva: culture dish or jar in raise newly hatched larvae, just to incubate the feed larva of ladybug, chrysopa septempunctata or expert on Japan Chrysopa of lepidopterous insects larva; Advanced age ladybug, chrysopa septempunctata or expert on Japan Chrysopa larva transfer in the breathable plastic box with 2 age the lepidopterous insects larva feed 20～40 lepidopterous larvaes of feeding every every day;
D. the processing of pupa: after treating that it pupates, beat easily down and put into culture dish, concentrate and put into the adult dependent insect cage, raise the back of sprouting wings; And
E. preservation of adult and release: store adult down at 12 ℃, the added body volume concentrations is 10% hydromel weekly, and storage life 3～5 months is treated to carry out when the field needs the field and discharged.
On the other hand, the present invention also provides a kind of method of pest control, and this method comprises that the ladybug, chrysopa septempunctata or the expert on Japan Chrysopa that utilize the method for the invention to raise are discharged into the field and/or the woods.
Preferably, described insect is selected from one or more in aphid, mite class, scale insect and the aleyrodid.
Compare with conventional art, method of the present invention is that feed is raised ladybug or Chrysopa on a large scale with the lepidopterous insects larva, the way that has replaced traditional aphid to raise natural enemy insect ladybug, Chrysopa with the plant cultivation, and this method is more time saving and energy saving, simple and easy to do.
Embodiment
Followingly the present invention is described with reference to specific embodiment.It will be appreciated by those skilled in the art that these embodiment only are used to illustrate purpose of the present invention, the scope that it does not limit the present invention in any way.
Embodiment
1. the raising of lepidopterous insects larva
Adopt the artificial feed prescription of inventor's invention, the prescription of described feed comprises by weight: 200 parts in 10 parts of casein, 20 parts in wheat germ powder, 20 parts of tomatoes, 3 parts of potassium sorbates, 0.2 part of vitamin C, 0.7 part of rapeseed oil, 2 parts in agar and water;
Its concrete preparation steps is as follows:
A. 20 parts tomato is broken into pasty state, add 200 parts water, the agar powder of 2 parts of weight and rapeseed oil, the limit is stirred and heated to boiling, and agar is dissolved fully;
B. in addition load weighted casein and wheat germ powder potassium sorbate vitamin etc. is put into a container mixing together; With
C. dissolving in the steps A is good tomato agar mixture is poured in the container of B while hot, and the material mixing among the step B in the tomato agar mixture, is solid mixture after the cooling, cuts apart to store the lepidopterous larvae of feeding again.
Raise beet armyworm with these artificial feed, larva is on average gone through 14 days phases, average pupa weighs 0.105 gram (not finding the population degradation phenomena more than 32 generations) beet armyworm of feeding, the beet armyworm adult feed above-mentioned feed in addition volumetric concentration be 10% hydromel, after adult lays eggs, ovum collecting is sterilized, wait to hatch, be transferred to after the hatching and add the artificial feed raising in the culture dish, along with the constantly additional artificial feed of the growth of larva, treat to change in the square box after it entered for 4 ages and raise, after pupating, sterilization is dried, and changes mate and oviposit to be sprouted wings in the adult dependent insect cage again over to.
2. the raising of grandis adults
In the dependent insect cage that lucite is made, raise ladybug,
Adopt the beet exigua larvae of 2 age ends to raise, at 25～27 ℃, under the condition of relative moisture 60～80% with imago breeding in dependent insect cage, the in addition sufficient beet exigua larvae every day artificial feed of beet exigua larvae in addition, beet exigua larvae can continue to get the food growth, every ladybug is approximately got the food beet exigua larvae of 20 2 age ends, and adult eclosion mate and oviposit after one week is laid eggs after 10～20 days in a large number.
3. the store collected of ovum
Put into egg holder or paper slip in the ladybug dependent insect cage, ladybug is liked laying eggs at the groove of egg holder, often changes the egg holder, and ladybug is preferred laying eggs in fresh egg holder.Adult lays eggs on egg holder or paper slip, after the collection, will directly hatch and raise or in storing 10 days under 8～12 ℃, take out when needing and hatch.Collect a large amount of ovum and can concentrate extensive raising.
4. the raising of larva
The airtight raising in culture dish or plastic tank of low age ladybug larva, add the beet exigua larvae and the feed thereof of just incubating, this feed is raised lepidopterous larvae for the artificial feed of inventor's invention, purpose is for allowing beet exigua larvae get anthropophagy worker's feed, the length of side is eaten on the limit, make it that superior food environment be arranged, obtain more sufficient lepidopterous larvae as food so that predator is better, the prescription of described artificial feed of the present invention comprises by weight: 10 parts of casein, 20 parts in wheat germ powder, 20 parts of tomatoes, 3 parts of potassium sorbates, 0.2 part of vitamin C, 0.7 part of rapeseed oil, 200 parts in 2 parts in agar and water;
Its concrete preparation steps is as follows:
A. 20 parts tomato is broken into pasty state, add 200 parts water, the agar powder of 2 parts of weight and rapeseed oil, the limit is stirred and heated to boiling, and agar is dissolved fully;
B. in addition load weighted casein and wheat germ powder potassium sorbate vitamin etc. is put into a container mixing together; With
C. dissolving in the steps A is good tomato agar mixture is poured in the container of B while hot, and the material mixing among the step B in the tomato agar mixture, is solid mixture after the cooling, cuts apart to store the lepidopterous larvae of feeding again.
The larva of every ladybug can add the beet armyworm newly hatched larvae more than 20, and 3 days can be without supplement feed.The ladybug larva was raised two days again at the beginning of replenishing 1 age Mo or 2 ages after 3 days, ladybug entered at the beginning of 3 age Mos or 4 ages, change in the big plastics square box and raise, increase the space, add again 2 age beet exigua larvae, predation every every day can reach 30～40 2 age beet exigua larvae, can change additional beet exigua larvae in per two days.Basically larva raising just can be pupated in 8～10 days.
3, the ladybug larva in 4 ages is raised in big plastic casing, and plastic casing is ventilated coated with gauze, raises with the beet exigua larvae of 2 age ends.As long as the generation of seldom killing each other of sufficient feed and space is arranged.
5. the management of pupa
The ladybug larvae pupation is beaten easily down two days later, puts into culture dish, puts into the dependent insect cage of adult again and treats directly to raise with the beet exigua larvae after pupa is sprouted wings.
6. the storage of adult
Under 12 ℃, the adult time-to-live of ladybug can reach 3-5 month, and is middle every additional 10% hydromel of 2 weeks.
One week of the adult eclosion back mate and oviposit of ladybug is laid eggs after 20 days in a large number.At 25～27 ℃, under the condition of relative moisture 60～80%, to hatch 3 days, incubation rate is 72.4%, 8～10 days larval phases,, pupation rate reaches 50～80%, 4～5 days pupa time.Eclosion rate 78%, adult survival 3～5 months.
Claims (10)
1. the method for artificial feeding ladybug or chrysopa septempunctata, this method may further comprise the steps:
(1) hatches the lepidopterous insects larva after the lepidopterous insects adult lays eggs, adopt the feed lepidopterous insects larva of feeding, this feed is counted by weight and is comprised: 200 parts in 10 parts of casein, 20 parts in wheat germ powder, 20 parts of tomatoes, 3 parts of potassium sorbates, 0.2 part of vitamin C, 0.7 part of rapeseed oil, 2 parts in agar and water;
(2) obtain grandis adults or chrysopa septempunctata adult with feed ladybug larva or chrysopa septempunctata larva of lepidopterous insects larva; And
(3) with lepidopterous insects larva feed grandis adults or chrysopa septempunctata adult, after laying eggs, adult hatches ladybug larva or chrysopa septempunctata larva.
2. method according to claim 1 is characterized in that, described step (3) may further comprise the steps:
I) under 25～27 ℃, relative moisture are 60～80% condition, with 2 age feed grandis adults or chrysopa septempunctata adult of lepidopterous insects larva, treat that adult lays eggs after 10～20 days;
II) collect egg hatch and go out ladybug larva or chrysopa septempunctata larva after, be to feed with the lepidopterous insects larva that just incubates under 60～80% the condition at 25～27 ℃, relative moisture with it; And
III) 3 or 4 age the ladybug larva or the chrysopa septempunctata larva with 2 age the lepidopterous insects larva feed.
3. the method for an artificial feeding expert on Japan Chrysopa, this method may further comprise the steps:
1) hatches the lepidopterous insects larva after the lepidopterous insects adult lays eggs, adopt the feed lepidopterous insects larva of feeding, this feed is counted by weight and is comprised: 200 parts in 10 parts of casein, 20 parts in wheat germ powder, 20 parts of tomatoes, 3 parts of potassium sorbates, 0.2 part of vitamin C, 0.7 part of rapeseed oil, 2 parts in agar and water;
2) obtain expert on Japan Chrysopa adult with the lepidopterous insects larva expert on Japan Chrysopa larva of feeding; And
3) with honey and the beer dried yeast powder expert on Japan Chrysopa adult of feeding, after laying eggs, adult hatches expert on Japan Chrysopa larva.
4. method according to claim 3 is characterized in that, described step 3) may further comprise the steps:
I) under 25～27 ℃, relative moisture are 60～80% condition,, treat that adult lays eggs after 10～20 days with honey and the beer dried yeast powder expert on Japan Chrysopa adult of feeding;
After ii) collecting egg hatch and going out expert on Japan Chrysopa larva, be to raise under 60～80% the condition at 25～27 ℃, relative moisture, feed with the lepidopterous insects larva that just incubates with it; And
Iii) 3 or 4 age expert on Japan Chrysopa larva with 2 age the lepidopterous insects larva feed.
5. according to each described method in the claim 1 to 4, it is characterized in that, in described step (1), with feed and the hydromel lepidopterous insects adult of feeding, this feed is counted by weight and is comprised: 200 parts in 10 parts of casein, 20 parts in wheat germ powder, 20 parts of tomatoes, 3 parts of potassium sorbates, 0.2 part of vitamin C, 0.7 part of rapeseed oil, 2 parts in agar and water; The volumetric concentration of described hydromel is 5～20%, is preferably 10%.
6. according to each described method in the claim 1 to 5, it is characterized in that, in described step (2), the ladybug larva that just incubates, chrysopa septempunctata larva or expert on Japan Chrysopa larva adopt the lepidopterous insects larva that just incubates to feed, 3 or 4 age ladybug larva, chrysopa septempunctata larva or expert on Japan Chrysopa larva adopt 2 age the lepidopterous insects larva feed.
7. according to each described method in the claim 1 to 6, it is characterized in that described lepidopterous insects is a beet armyworm.
8. the method for an artificial feeding ladybug, chrysopa septempunctata or expert on Japan Chrysopa, this method may further comprise the steps:
A. the raising of lepidopterous insects
Adopt the feed lepidopterous larvae of feeding, and adopt this feed and the hydromel lepidoptera imago of feeding, treat to hatch larva after this adult lays eggs, adopt this feed larva of feeding once more, grow up to adult after pupating, this feed is counted by weight and is comprised: 200 parts in 10 parts of casein, 20 parts in wheat germ powder, 20 parts of tomatoes, 3 parts of potassium sorbates, 0.2 part of vitamin C, 0.7 part of rapeseed oil, 2 parts in agar and water; The volumetric concentration of hydromel is 5～20%, is preferably 10%.
B. the raising of ladybug, chrysopa septempunctata or expert on Japan Chrysopa
A. imago breeding: at 25～27 ℃, under 60～80% the relative moisture condition, in dependent insect cage, raise adult, the adult of ladybug or chrysopa septempunctata add every day 2 age lepidopterous larvae feed, with honey and the beer dried yeast powder expert on Japan Chrysopa of feeding, treat to lay eggs after 10～20 days;
B. the collection of ovum and preservation: adopt egg holder or paper slip to put into the adult dependent insect cage, adult lays eggs on the groove or paper slip of egg holder, changes egg holder or paper slip, collects ovum and is stored in them in 10～12 ℃ of incubators or directly 25～27 ℃ of hatchings;
C. the raising of larva: in culture dish or jar, raise newly hatched larvae, just to incubate the feed larva of ladybug, chrysopa septempunctata or expert on Japan Chrysopa of lepidopterous insects larva, the ladybug at advanced age, chrysopa septempunctata or expert on Japan Chrysopa larva transfer to feed in the breathable plastic box 2 age the lepidopterous insects larva, 20～40 lepidopterous larvaes of feeding every every day;
D. the processing of pupa: after treating that it pupates, beat easily down and put into culture dish, concentrate and put into the adult dependent insect cage, raise the back of sprouting wings; And
E. preservation of adult and release: store adult down at 12 ℃, the added body volume concentrations is 10% hydromel weekly, and storage life 3～5 months is treated to carry out when the field needs the field and discharged.
9. the method for a pest control, this method comprise and will be discharged into the field and/or the woods according to ladybug, chrysopa septempunctata or the expert on Japan Chrysopa that each described method in the claim 1 to 8 is raised.
10. method according to claim 9 is characterized in that, described insect is selected from one or more in aphid, mite class, scale insect and the aleyrodid.
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| CN2009100924899A CN102017929B (en) | 2009-09-10 | 2009-09-10 | Method for artificially feeding ladybirds or lacewings by using lepidoptera insect larvae |
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| CN2009100924899A CN102017929B (en) | 2009-09-10 | 2009-09-10 | Method for artificially feeding ladybirds or lacewings by using lepidoptera insect larvae |
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| CN1285260C (en) * | 2005-01-26 | 2006-11-22 | 北京市农林科学院 | Artificial progagation method for ladybird beetle and lacewing fly |
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