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CN103782969A - Method for breeding trichogramma pintoi indoors and corcyra cephalonica raising fodder - Google Patents

Method for breeding trichogramma pintoi indoors and corcyra cephalonica raising fodder Download PDF

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Publication number
CN103782969A
CN103782969A CN201410074195.4A CN201410074195A CN103782969A CN 103782969 A CN103782969 A CN 103782969A CN 201410074195 A CN201410074195 A CN 201410074195A CN 103782969 A CN103782969 A CN 103782969A
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feed
parts
breeding
rice moth
trichogramma
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CN103782969B (en
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李德伟
邓艳
蒋学建
常明山
吴耀军
黄华艳
赵程劼
秦元丽
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Hunan Academy of Forestry
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Hunan Academy of Forestry
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Abstract

本发明提供了一种室内繁殖暗黑赤眼蜂的方法及米蛾饲养饲料,米蛾饲养饲料分A饲料和B饲料,A饲料的原料成份及重量份数:粗麦麸50份、玉米粉40份、面粉10份和水5份;B饲料的原料成份及重量份数:粗麦麸50份、玉米粉40份、面粉5份、鱼粉3份、酵母粉2份和水5份。繁殖方法以米蛾卵为中间寄主,采用多种饲料配制米蛾不同时期幼虫的配方饲料,通过配方饲料饲养米蛾幼虫使成虫羽化整齐,产卵量高,采用大试管接入卵卡的方式来大量接种繁殖赤眼蜂。本发明不需大型仪器,所用繁殖材料简单易得,操作简单,实用性强,对环境友好、无污染,所生产的暗黑赤眼蜂可用于多种农林害虫的生物防治,具有较好的经济、生态和社会效益。The invention provides a method for indoor propagation of Trichogramma diabloca and feed for rice moth. The feed for rice moth is divided into feed A and feed B. The raw material components and parts by weight of feed A are: 50 parts of coarse wheat bran, 40 parts of corn flour 10 parts of flour and 5 parts of water; the ingredients and parts by weight of feed B: 50 parts of coarse wheat bran, 40 parts of corn flour, 5 parts of flour, 3 parts of fish meal, 2 parts of yeast powder and 5 parts of water. The breeding method takes rice moth eggs as the intermediate host, and adopts a variety of feeds to prepare formula feeds for rice moth larvae at different stages, and feeds rice moth larvae through formula feeds to make adults emerge neatly and have high egg production. The method of inserting egg cards into large test tubes is adopted. To inoculate and breed Trichogramma in large quantities. The present invention does not need large-scale instruments, the propagation materials used are simple and easy to obtain, simple to operate, strong in practicability, friendly to the environment and free from pollution. , ecological and social benefits.

Description

A kind of method of indoor propagation Trichogramma pintoi and rice moth culturing feed
Technical field
The invention belongs to agriculture and forestry injurious insect biological prevention field, relate to a kind of method and rice moth culturing feed of indoor propagation Trichogramma pintoi.
Background technology
Oophagous trichogrammae ( trichogrammaspp.) discovery existing more than 100 year apart from the present, ovum that can parasitic multiple lepidopterous insects, is a class ovum parasite most widely used in whole world biological control of insect pests.China has just carried out application study to oophagous trichogrammae since nineteen thirties, from 1934, Zhu Ruzuo, Hu Yongxi stop to the seventies from having found oophagous trichogrammae in pine tag worm's ovum at the Tang Xixian of Zhejiang Province, and Pu host, large scale artificial breeding and the field bulk storage effects etc. to oophagous trichogrammae such as dragon of stinging have been carried out systematic research.After the eighties, China's oophagous trichogrammae research and application technology have obtained further reinforcement, and oophagous trichogrammae classification, behavior, physiological ecological and artificial host egg's application aspect has been carried out a large amount of work.Along with improving constantly of the development of sustainable agriculture and the requirement of pollution-free green food industry, this biological prevention that is master with natural enemy is more and more accepted by vast farmers.Trichogramma pintoi is the natural enemy that a class has value very much, can be used for the multiple agriculture and forestry injurious insect biological controls such as cotton bollworm, beet webworm, cutworm, steinernema.The institute of agricultural sciences of Yuncheng Prefecture of Shanxi Province to Trichogramma pintoi biological characteristics seek peace parasitic cotton bollworm condition survey observe; Wang Fucheng, a comb instrument produces the research of the Trichogramma pintoi of both sexes filial generation to parthenogenesis; Ma Deying etc. prevent and treat Cotton Boll Worm Populations From Xinjiang Uygur Autonomous Region Preliminary Report on Experiment to Trichogramma pintoi, and have carried out the impact research of term refrigeration on different lines Trichogramma pintoi; Tian Xiaoxia etc. find the parasitic beet webworm ovum of Trichogramma pintoi first in China; Shen Jian, is permitted to build up the Army to wait and has been carried out the impact of ultraviolet processing oriental fruit moth ovum on Trichogramma pintoi parasitism and emergence, impact and the Trichogramma pintoi parasitization research to oriental fruit moth ovum of high temperature stress on Trichogramma pintoi parasitic functional response.And for the artificial propagation techniques of Trichogramma pintoi except coroner all auspicious grade introduced and a kind of method of utilizing oriental fruit moth ovum indoor propagation Trichogramma pintoi, do not seen other report, and the method is difficult to large-scale production and is applied to biological control of insect pests.
Summary of the invention
The object of this invention is to provide a kind of efficiently in the indoor method of utilizing rice moth egg to carry out amount reproduction Trichogramma pintoi; Another object of the present invention is to provide a kind of rice moth feed efficiently.
In order to achieve the above object, the present invention adopts following technical scheme:
A rice moth culturing feed for indoor propagation Trichogramma pintoi, rice moth culturing feed divides A feed and B feed, and its preparation raw material composition and parts by weight are respectively:
Raw material composition and the parts by weight of A feed:
5 parts, 50 parts of brown shorts, 40 parts of corn flour, 10 parts, flour and water;
Raw material composition and the parts by weight of B feed:
5 parts, 50 parts of brown shorts, 40 parts of corn flour, 5 parts, flour, 3 parts of fish meal, 2 parts of dusty yeasts and water.
Described A feed and the preparation method of B feed be respectively:
(1) preparation of A feed: first the brown shorts of A feedstuff composition, corn flour, flour are put into disinfection cabinet and heat, temperature is sterilized at keeping 60-90 ℃ 4-5 hour, kill germ, insect, evil mite and ovum thereof in feed, again brown shorts and water are fully mixed thoroughly, then added corn flour and flour fully to mix thoroughly again and obtain A feed;
(2) preparation of B feed: first the brown shorts of B feedstuff composition, corn flour, flour, fish meal are put into disinfection cabinet and heat, temperature is sterilized at keeping 60-90 ℃ 4-5 hour, kill germ, insect, evil mite and ovum thereof in feed, again brown shorts and water are fully mixed thoroughly, then added corn flour, flour, fish meal fully to mix thoroughly again and obtain B feed together with dusty yeast.
A kind of method of indoor propagation Trichogramma pintoi, in mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, to be returned indoor collection Trichogramma pintoi as honeybee kind provenance by the ovum collection of Trichogramma, breed in a large number as vector using rice moth egg indoor, propagation method comprises the following steps:
(1) breeding of rice moth: when rice moth inoculation, at the thick A feed of plastic crate middle berth one deck 1.1-1.2 cm, on feed, evenly spill rice moth kind ovum, free-and-easy one deck just covers the flour bisque of ovum grain again, is placed in rice moth and raises indoor feeding, and the larva after hatching starts feeding after eating out thickly dotted aperture, within first 15 days, throw in A feed in inoculation, throw in afterwards B feed, within every 3 days, throw a defective material, input amount is that the larva appetite of 3 days is until larvae pupation; Pupa is collected adult after sprouting wings and is placed in the cage of laying eggs and lays eggs, and collects rice moth egg every day, and part is directly used in the breeding of rice moth, and remainder kills and performs mark after embryo and put into the refrigerator of 3-5 ℃ and preserve with ultraviolet light, is made into rice moth egg card before numerous honeybee, for subsequent use;
(2) field bee kind: in mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, gather by the ovum of Trichogramma, return indoor bottle and collect Trichogramma pintoi as honeybee kind provenance, in the bottle of collecting honeybee kind, access rice moth egg card, parasitism is bred into oophagous trichogrammae breeding kind of honeybee card, for subsequent use;
(3) breeding of oophagous trichogrammae: 1 oophagous trichogrammae breeding kind of honeybee card is put into breeding bee tube numerous honeybee is indoor, clog the mouth of pipe with cotton plug, on honeybee card, snap into and in breeding bee tube, allow Trichogramma at 8 rice moth eggs of transfer room access when the bee eclosion of 60-70 %, put into the filter paper bar nursing of sweeping 10 % hydromels simultaneously, when access ovum card, the other end of the mouth of pipe is to light, and anti-kind of honeybee escapes from, after having inoculated, all breeding bee tubes that accessed ovum card are being palletized and covering shading with black cloth, then move into that numerous honeybee is indoor to lay eggs, after 8-14 hour, in transfer room, change to 8 new rice moth egg cards with same inoculation method, every breeding kind of honeybee card can connect 16 rice moth egg cards, postvaccinal rice moth egg card is standing and is being put in capsule and moves into that numerous honeybee is indoor continues to breed 4-5 days, then the refrigerator of putting into 3-5 ℃ carries out short-term preservation, during with honeybee, shift to an earlier date 4-7 days and take out and be placed in the indoor cultivation of numerous honeybee from refrigerator, the available expansion of honeybee card after cultivation is numerous or for biological control of insect pests.
Described rice moth receptacle control indoor temperature is 25-28 ℃, and relative moisture is 60-85 %.
It is 25-28 ℃ that indoor temperature is controlled in described numerous honeybee chamber, and relative moisture is 70-85 %.
Described breeding bee tube is the Boiling tube of diameter 30 mm × length 20 cm.
Described rice moth egg card is that the sticky rice moth egg of the strip label card of 2 cm × 4 cm is made.
Advantage of the present invention and beneficial effect have:
(1) Trichogramma pintoi that the present invention breeds is the extensive natural enemy insect of a class host, can be used in the biological control of multiple agriculture and forestry injurious insect.
(2) in rice moth of the present invention breeding, in larvae feeds, added fish meal, dusty yeast makes rice moth adult eclosion more neat, egg laying amount is higher.
(3) make the parasitic rate of oophagous trichogrammae higher with the numerous honeybee of Boiling tube, mean parasitized rate reaches more than 90%.
(4) the present invention is without large-scale instrument, and propagating materials used is easily obtained, simple to operate, practical.
(5) the present invention is environmentally friendly, pollution-free, is suitable for application and promotes, and has good social benefit.
Embodiment
Below in conjunction with specific embodiment, the present invention is further described.
embodiment 1:
One, the rice moth culturing feed of breeding Trichogramma pintoi:
Raw material composition and the parts by weight of A feed:
5 parts, 50 parts of brown shorts, 40 parts of corn flour, 10 parts, flour and water;
Raw material composition and the parts by weight of B feed:
5 parts, 50 parts of brown shorts, 40 parts of corn flour, 5 parts, flour, 3 parts of fish meal, 2 parts of dusty yeasts and water.
Described A feed and the preparation method of B feed be respectively:
(1) preparation of A feed: first the brown shorts of A feedstuff composition, corn flour, flour are put into disinfection cabinet and heat, temperature is sterilized 5 hours at keeping 60 ℃, kill germ, insect, evil mite and ovum thereof in feed, again brown shorts and water are fully mixed thoroughly, then added corn flour and flour fully to mix thoroughly again and obtain A feed;
(2) preparation of B feed: first the brown shorts of B feedstuff composition, corn flour, flour, fish meal are put into disinfection cabinet and heat, temperature is sterilized 5 hours at keeping 60 ℃, kill germ, insect, evil mite and ovum thereof in feed, again brown shorts and water are fully mixed thoroughly, then added corn flour, flour, fish meal fully to mix thoroughly again and obtain B feed together with dusty yeast.
Two, the method for indoor propagation Trichogramma pintoi:
In mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, using being returned indoor collection Trichogramma pintoi as honeybee kind provenance by the ovum collection of Trichogramma, to breed in a large number as vector using rice moth egg indoor, propagation method comprises the following steps:
(1) breeding of rice moth: when rice moth inoculation, at the thick A feed of plastic crate middle berth one deck 1.1cm, on feed, evenly spill rice moth kind ovum, free-and-easy one deck just covers the flour bisque of ovum grain again, be placed in rice moth and raise indoor feeding, controlling indoor temperature is 25 ℃, relative moisture is 70 %, larva after hatching starts feeding after eating out thickly dotted aperture, within first 15 days, throw in A feed in inoculation, throw in afterwards B feed, within every 3 days, throw a defective material, input amount is that the larva appetite of 3 days is until larvae pupation; The rear collection of pupa emergence adult is placed in the cage of laying eggs and lays eggs, collect rice moth egg every day, part is directly used in the breeding of rice moth, remainder kills and performs mark after embryo and put into the refrigerator of 3 ℃ and preserve with ultraviolet light, the sticky rice moth egg of strip label card with 2 cm × 4 cm before numerous honeybee is made into rice moth egg card, for subsequent use;
(3) field bee kind: in mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, gather by the ovum of Trichogramma, return indoor bottle and collect Trichogramma pintoi as honeybee kind provenance, in the bottle of collecting honeybee kind, access rice moth egg card, parasitism is bred into oophagous trichogrammae breeding kind of honeybee card, for subsequent use;
(4) breeding of oophagous trichogrammae: at the indoor breeding bee tube of 1 oophagous trichogrammae breeding kind of honeybee card being put into diameter 30mm × long 20cm of numerous honeybee, clog the mouth of pipe with cotton plug, on honeybee card, snap into and in breeding bee tube, allow Trichogramma at 8 rice moth eggs of transfer room access when 60% bee eclosion, put into the filter paper bar nursing of sweeping 10 % hydromels simultaneously, when access ovum card, the other end of the mouth of pipe is to light, and anti-kind of honeybee escapes from, after having inoculated, all breeding bee tubes that accessed ovum card are being palletized and covering shading with black cloth, then move into that numerous honeybee is indoor to lay eggs, controlling indoor temperature is 25 ℃, relative moisture is 70 %, after 8 hours, in transfer room, change to 8 new rice moth egg cards with same inoculation method, every breeding kind of honeybee card can connect 16 rice moth egg cards, postvaccinal rice moth egg card is standing and is being put in capsule and moves into that numerous honeybee is indoor to be continued to breed 4 days, mean parasitized rate reaches 90.6%, then the refrigerator of parasitic rice moth egg card being put into 3 ℃ carries out short-term preservation, during with honeybee, within 7 days, take out and be placed in the indoor cultivation of numerous honeybee from refrigerator in advance, the available expansion of honeybee card after cultivation is numerous or for biological control of insect pests.
embodiment 2:
One, the rice moth culturing feed of breeding Trichogramma pintoi:
Raw material composition and the parts by weight of A feed:
5 parts, 50 parts of brown shorts, 40 parts of corn flour, 10 parts, flour and water;
Raw material composition and the parts by weight of B feed:
5 parts, 50 parts of brown shorts, 40 parts of corn flour, 5 parts, flour, 3 parts of fish meal, 2 parts of dusty yeasts and water.
Described A feed and the preparation method of B feed be respectively:
(1) preparation of A feed: first the brown shorts of A feedstuff composition, corn flour, flour are put into disinfection cabinet and heat, temperature is sterilized 5 hours at keeping 70 ℃, kill germ, insect, evil mite and ovum thereof in feed, again brown shorts and water are fully mixed thoroughly, then added corn flour and flour fully to mix thoroughly again and obtain A feed;
(2) preparation of B feed: first the brown shorts of B feedstuff composition, corn flour, flour, fish meal are put into disinfection cabinet and heat, temperature is sterilized 5 hours at keeping 70 ℃, kill germ, insect, evil mite and ovum thereof in feed, again brown shorts and water are fully mixed thoroughly, then added corn flour, flour, fish meal fully to mix thoroughly again and obtain B feed together with dusty yeast.
Two, the method for indoor propagation Trichogramma pintoi:
In mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, using being returned indoor collection Trichogramma pintoi as honeybee kind provenance by the ovum collection of Trichogramma, to breed in a large number as vector using rice moth egg indoor, propagation method comprises the following steps:
(1) breeding of rice moth: when rice moth inoculation, at the thick A feed of plastic crate middle berth one deck 1.1cm, on feed, evenly spill rice moth kind ovum, free-and-easy one deck just covers the flour bisque of ovum grain again, be placed in rice moth and raise indoor feeding, controlling indoor temperature is 26 ℃, relative moisture is 75 %, larva after hatching starts feeding after eating out thickly dotted aperture, within first 15 days, throw in A feed in inoculation, throw in afterwards B feed, within every 3 days, throw a defective material, input amount is that the larva appetite of 3 days is until larvae pupation; The rear collection of pupa emergence adult is placed in the cage of laying eggs and lays eggs, collect rice moth egg every day, part is directly used in the breeding of rice moth, remainder kills and performs mark after embryo and put into the refrigerator of 4 ℃ and preserve with ultraviolet light, the sticky rice moth egg of strip label card with 2 cm × 4 cm before numerous honeybee is made into rice moth egg card, for subsequent use;
(3) field bee kind: in mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, gather by the ovum of Trichogramma, return indoor bottle and collect Trichogramma pintoi as honeybee kind provenance, in the bottle of collecting honeybee kind, access rice moth egg card, parasitism is bred into oophagous trichogrammae breeding kind of honeybee card, for subsequent use;
(4) breeding of oophagous trichogrammae: at the indoor breeding bee tube of 1 oophagous trichogrammae breeding kind of honeybee card being put into diameter 30mm × long 20cm of numerous honeybee, clog the mouth of pipe with cotton plug, on honeybee card, snap into and in breeding bee tube, allow Trichogramma at 8 rice moth eggs of transfer room access when the bee eclosion of 65 %, put into the filter paper bar nursing of sweeping 10 % hydromels simultaneously, when access ovum card, the other end of the mouth of pipe is to light, and anti-kind of honeybee escapes from, after having inoculated, all breeding bee tubes that accessed ovum card are being palletized and covering shading with black cloth, then move into that numerous honeybee is indoor to lay eggs, controlling indoor temperature is 26 ℃, relative moisture is 75 %, after 10 hours, in transfer room, change to 8 new rice moth egg cards with same inoculation method, every breeding kind of honeybee card can connect 16 rice moth egg cards, postvaccinal rice moth egg card is standing and is being put in capsule and moves into that numerous honeybee is indoor to be continued to breed 5 days, mean parasitized rate reaches 92.1%, then the refrigerator of parasitic rice moth egg card being put into 4 ℃ carries out short-term preservation, during with honeybee, within 5 days, take out and be placed in the indoor cultivation of numerous honeybee from refrigerator in advance, the available expansion of honeybee card after cultivation is numerous or for biological control of insect pests.
embodiment 3:
One, the rice moth culturing feed of breeding Trichogramma pintoi:
Raw material composition and the parts by weight of A feed:
5 parts, 50 parts of brown shorts, 40 parts of corn flour, 10 parts, flour and water;
Raw material composition and the parts by weight of B feed:
5 parts, 50 parts of brown shorts, 40 parts of corn flour, 5 parts, flour, 3 parts of fish meal, 2 parts of dusty yeasts and water.
Described A feed and the preparation method of B feed be respectively:
(1) preparation of A feed: first the brown shorts of A feedstuff composition, corn flour, flour are put into disinfection cabinet and heat, temperature is sterilized 4 hours at keeping 90 ℃, kill germ, insect, evil mite and ovum thereof in feed, again brown shorts and water are fully mixed thoroughly, then added corn flour and flour fully to mix thoroughly again and obtain A feed;
(2) preparation of B feed: first the brown shorts of B feedstuff composition, corn flour, flour, fish meal are put into disinfection cabinet and heat, temperature is sterilized 4 hours at keeping 90 ℃, kill germ, insect, evil mite and ovum thereof in feed, again brown shorts and water are fully mixed thoroughly, then added corn flour, flour, fish meal fully to mix thoroughly again and obtain B feed together with dusty yeast.
Two, the method for indoor propagation Trichogramma pintoi:
In mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, using being returned indoor collection Trichogramma pintoi as honeybee kind provenance by the ovum collection of Trichogramma, to breed in a large number as vector using rice moth egg indoor, propagation method comprises the following steps:
(1) breeding of rice moth: when rice moth inoculation, at the thick A feed of plastic crate middle berth one deck 1.2 cm, on feed, evenly spill rice moth kind ovum, free-and-easy one deck just covers the flour bisque of ovum grain again, be placed in rice moth and raise indoor feeding, controlling indoor temperature is 28 ℃, relative moisture is 80 %, larva after hatching starts feeding after eating out thickly dotted aperture, within first 15 days, throw in A feed in inoculation, throw in afterwards B feed, within every 3 days, throw a defective material, input amount is that the larva appetite of 3 days is until larvae pupation; The rear collection of pupa emergence adult is placed in the cage of laying eggs and lays eggs, collect rice moth egg every day, part is directly used in the breeding of rice moth, remainder kills and performs mark after embryo and put into the refrigerator of 5 ℃ and preserve with ultraviolet light, the sticky rice moth egg of strip label card with 2 cm × 4 cm before numerous honeybee is made into rice moth egg card, for subsequent use;
(3) field bee kind: in mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, gather by the ovum of Trichogramma, return indoor bottle and collect Trichogramma pintoi as honeybee kind provenance, in the bottle of collecting honeybee kind, access rice moth egg card, parasitism is bred into oophagous trichogrammae breeding kind of honeybee card, for subsequent use;
(4) breeding of oophagous trichogrammae: at the indoor breeding bee tube of 1 oophagous trichogrammae breeding kind of honeybee card being put into diameter 30mm × long 20cm of numerous honeybee, clog the mouth of pipe with cotton plug, on honeybee card, snap into and in breeding bee tube, allow Trichogramma at 8 rice moth eggs of transfer room access when 70% bee eclosion, put into the filter paper bar nursing of sweeping 10 % hydromels simultaneously, when access ovum card, the other end of the mouth of pipe is to light, and anti-kind of honeybee escapes from, after having inoculated, all breeding bee tubes that accessed ovum card are being palletized and covering shading with black cloth, then move into that numerous honeybee is indoor to lay eggs, controlling indoor temperature is 28 ℃, relative moisture is 80 %, after 12 hours, in transfer room, change to 8 new rice moth egg cards with same inoculation method, every breeding kind of honeybee card can connect 16 rice moth egg cards, postvaccinal rice moth egg card is standing and is being put in capsule and moves into that numerous honeybee is indoor to be continued to breed 4 days, mean parasitized rate reaches 91.3%, then the refrigerator of parasitic rice moth egg card being put into 5 ℃ carries out short-term preservation, during with honeybee, within 6 days, take out and be placed in the indoor cultivation of numerous honeybee from refrigerator in advance, the available expansion of honeybee card after cultivation is numerous or for biological control of insect pests.

Claims (7)

1.一种室内繁殖暗黑赤眼蜂的米蛾饲养饲料,其特征在于:米蛾饲养饲料分A饲料和B饲料,其配制原料成份及重量份数分别为: 1. a rice moth raising feed for indoor breeding Trichogramma diablo, characterized in that: the rice moth raising feed is divided into A feed and B feed, and its preparation raw material composition and parts by weight are respectively: A饲料的原料成份及重量份数: Raw material composition and parts by weight of A feed: 粗麦麸50份、玉米粉40份、面粉10份和水5份; 50 parts of coarse wheat bran, 40 parts of corn flour, 10 parts of flour and 5 parts of water; B饲料的原料成份及重量份数: Raw material composition and parts by weight of B feed: 粗麦麸50份、玉米粉40份、面粉5份、鱼粉3份、酵母粉2份和水5份。 50 parts of coarse wheat bran, 40 parts of corn flour, 5 parts of flour, 3 parts of fish meal, 2 parts of yeast powder and 5 parts of water. 2.根据权利要求1所述的一种室内繁殖暗黑赤眼蜂的米蛾饲养饲料,其特征在于:所述的A饲料和B饲料的制备方法分别是: 2. a kind of rice moth breeding feed for indoor propagation of Trichogramma Diablo according to claim 1, is characterized in that: the preparation method of described A feed and B feed is respectively: (1)A饲料的制备:先将A饲料原料成份的粗麦麸、玉米粉、面粉放入消毒柜中加热,温度保持60-90℃下消毒4-5个小时,杀死饲料中的病菌、害虫、害螨及其卵,再将粗麦麸和水充分拌匀,然后加入玉米粉和面粉再充分拌匀得到A饲料; (1) Preparation of Feed A: Put the coarse wheat bran, cornmeal and flour, which are the raw materials of Feed A, into a disinfection cabinet to heat, and keep the temperature at 60-90°C for 4-5 hours to kill the germs in the feed. , pests, pest mites and their eggs, then fully mix the coarse wheat bran and water, then add corn flour and flour and fully mix to obtain A feed; (2)饲料的制备:先将B饲料原料成份的粗麦麸、玉米粉、面粉、鱼粉放入消毒柜中加热,温度保持60-90℃下消毒4-5个小时,杀死饲料中的病菌、害虫、害螨及其卵,再将粗麦麸和水充分拌匀,然后加入玉米粉、面粉、鱼粉和酵母粉一起再充分拌匀得到B饲料。 (2) Feed preparation: Put the coarse wheat bran, corn flour, flour, and fish meal, which are the raw materials of feed B, into a disinfection cabinet to heat, and keep the temperature at 60-90°C for 4-5 hours to kill the bacteria in the feed. Bacteria, pests, harmful mites and their eggs, then fully mix coarse wheat bran and water, then add corn flour, flour, fish meal and yeast powder and fully mix to obtain B feed. 3.一种室内繁殖暗黑赤眼蜂的方法,其特征在于:在红树林桐花树毛颚小卷蛾成虫产卵高峰期,将被赤眼蜂寄生的卵采集回室内收集暗黑赤眼蜂作为蜂种种源,在室内以米蛾卵作为中间寄主进行大量繁育,繁殖方法包括以下步骤: 3. A method for indoor propagation of Trichogramma, characterized in that: during the peak period of laying eggs of the adult Trichogramma moth in the mangrove tree, the ovum parasitized by Trichogramma is collected back indoors to collect Trichogramma As the bee seed source, a large amount of breeding is carried out indoors with rice moth eggs as intermediate hosts. The breeding method includes the following steps: (1)米蛾的繁殖:米蛾接种时,在塑料筐中铺一层1.1-1.2cm厚的A饲料,在饲料上均匀洒上米蛾种卵,再飘洒一层刚好覆盖卵粒的面粉粉层,置于米蛾饲养室内饲养,待孵化后的幼虫食出密密麻麻的小孔后开始投食,在接种前15天投放A饲料,之后投放B饲料,每3天投一次料,投入量为幼虫3天的食量直至幼虫化蛹;蛹羽化后收集成虫置于产卵笼中产卵,每天收集米蛾卵,部分直接用于米蛾的繁殖,剩余部分用紫光灯杀胚后作好标记放入3-5 ℃的冰箱中保存,繁蜂前制作成米蛾卵卡,备用; (1) Propagation of the rice moth: When the rice moth is inoculated, spread a layer of 1.1-1.2cm thick A feed in the plastic basket, evenly sprinkle the rice moth eggs on the feed, and then sprinkle a layer of flour just covering the eggs Powder layer, placed in rice moth breeding room for feeding, after the hatched larvae ate out of the densely packed small holes, they began to feed. Feed A was fed 15 days before inoculation, and feed B was fed after that. Feed was fed once every 3 days. Food intake for the larvae for 3 days until the larvae pupate; after the pupae emerge, collect the adults and place them in the egg-laying cage to lay eggs, collect the eggs of the rice moth every day, some of them are directly used for the reproduction of the rice moth, and the rest are prepared after killing the embryos with a purple light lamp Mark it and store it in a refrigerator at 3-5°C. Make rice moth egg cards before multiplying the bees and keep them for later use; (2)采集蜂种:在红树林桐花树毛颚小卷蛾成虫产卵高峰期,采集被赤眼蜂寄生的卵,回室内用瓶子收集暗黑赤眼蜂作为蜂种种源,在收集蜂种的瓶子中接入米蛾卵卡,寄生繁育成赤眼蜂繁殖种蜂卡,备用; (2) Collection of bee species: During the peak oviposition period of the adults of the mangrove tung tree, Trichogramma trichognathus, collect the eggs parasitized by Trichogramma, and return to the room to collect Trichogramma diablo as the source of bee species. The rice moth egg card is inserted into the bottle of planting, and the parasitic breeding becomes the Trichogramma breeding species bee card, which is used for standby; (3)赤眼蜂的繁殖:在繁蜂室内将1张赤眼蜂繁殖种蜂卡放入繁蜂管中,用棉花塞塞住管口,待蜂卡上60-70 %的蜂羽化时在接种室接入8张米蛾卵卡到繁蜂管内让赤眼蜂寄生,同时放入扫有10 %蜂蜜水的滤纸条喂养,接入卵卡时管口的另一端对光,防种蜂逃出;接种完成后,将所有已接入卵卡的繁蜂管用托盘装着并用黑布覆盖遮光,然后移入繁蜂室内产卵,8-14小时后在接种室内以同样接种方法换入8张新的米蛾卵卡,每张繁殖种蜂卡可接16张米蛾卵卡,接种后的米蛾卵卡立着放于小盒内并移入繁蜂室内继续繁育4-5天,然后放入3-5 ℃的冰箱中进行短期保存,用蜂时提前4-7天从冰箱拿出置于繁蜂室内培养,培养后的蜂卡可用扩繁或用于害虫生物防治。 (3) Propagation of Trichogramma: Put a Trichogramma breeding seed card into the bee breeding tube in the bee breeding room, plug the tube opening with a cotton plug, and wait until 60-70% of the bees on the bee card emerge Insert 8 rice moth egg cards in the inoculation room into the bee breeding tube to let the Trichogramma parasitize. At the same time, put the filter paper strips with 10% honey water for feeding. When the egg cards are inserted, the other end of the tube mouth is facing the light to prevent Breeding bees escape; after the inoculation is completed, put all the bee breeding tubes that have been inserted into the egg card in trays and cover them with black cloth to shade them, then move them into the bee breeding room to lay eggs, and replace them in the inoculation room with the same inoculation method after 8-14 hours 8 new rice moth egg cards, each breeding bee card can be connected to 16 rice moth egg cards, the inoculated rice moth egg cards are placed in a small box and moved into the bee breeding room to continue breeding for 4-5 days, Then put it in a refrigerator at 3-5°C for short-term storage. When using bees, take them out of the refrigerator 4-7 days in advance and place them in the breeding room for cultivation. The cultivated bee cards can be used for propagation or biological control of pests. 4.根据权利要求3所述一种室内繁殖暗黑赤眼蜂的方法,其特征在于:所述的米蛾饲养室控制室内温度为25-28 ℃,相对湿度为60-85 %。 4. the method for a kind of indoor breeding Trichogramma diablo according to claim 3 is characterized in that: described rice moth breeding room control indoor temperature is 25-28 ℃, and relative humidity is 60-85%. 5.根据权利要求3所述一种室内繁殖暗黑赤眼蜂的方法,其特征在于:所述的繁蜂室控制室内温度为25-28 ℃,相对湿度为70-85 %。 5. according to claim 3, a kind of method for indoor breeding Trichogramma diablo, is characterized in that: the control indoor temperature of described beehive chamber is 25-28 ℃, and relative humidity is 70-85%. 6.根据权利要求3所述一种室内繁殖暗黑赤眼蜂的方法,其特征在于:所述的繁蜂管为直径30 mm×长20 cm的大试管。 6. according to the method for a kind of indoor breeding Trichogramma diablo according to claim 3, it is characterized in that: described numerous bee tube is the big test tube of diameter 30 mm * long 20 cm. 7.根据权利要求3所述一种室内繁殖暗黑赤眼蜂的方法,其特征在于:所述的米蛾卵卡为2 cm×4 cm的长条形标签卡粘米蛾卵制成。 7. according to the described method of a kind of indoor breeding Trichogramma Diablo according to claim 3, it is characterized in that: described rice moth ovum card is the strip label card sticky rice moth egg of 2 cm * 4 cm and is made.
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CN110973074A (en) * 2020-01-10 2020-04-10 夏鹏亮 Cultivation method and device of citrus mealybug and live feed for natural enemy insects
CN111226872A (en) * 2020-03-19 2020-06-05 湖南省林业科学院 A kind of method for indoor mass rearing of Helicoverpa armigera
CN116349656A (en) * 2023-05-26 2023-06-30 云南省烟草公司昆明市公司 Method for large-scale propagation of noctuid black egg bees
CN116349656B (en) * 2023-05-26 2023-08-22 云南省烟草公司昆明市公司 Method for large-scale propagation of noctuid black egg bees
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