Summary of the invention
The object of this invention is to provide a kind of efficiently in the indoor method of utilizing rice moth egg to carry out amount reproduction Trichogramma pintoi; Another object of the present invention is to provide a kind of rice moth feed efficiently.
In order to achieve the above object, the present invention adopts following technical scheme:
A rice moth culturing feed for indoor propagation Trichogramma pintoi, rice moth culturing feed divides A feed and B feed, and its preparation raw material composition and parts by weight are respectively:
Raw material composition and the parts by weight of A feed:
5 parts, 50 parts of brown shorts, 40 parts of corn flour, 10 parts, flour and water;
Raw material composition and the parts by weight of B feed:
5 parts, 50 parts of brown shorts, 40 parts of corn flour, 5 parts, flour, 3 parts of fish meal, 2 parts of dusty yeasts and water.
Described A feed and the preparation method of B feed be respectively:
(1) preparation of A feed: first the brown shorts of A feedstuff composition, corn flour, flour are put into disinfection cabinet and heat, temperature is sterilized at keeping 60-90 ℃ 4-5 hour, kill germ, insect, evil mite and ovum thereof in feed, again brown shorts and water are fully mixed thoroughly, then added corn flour and flour fully to mix thoroughly again and obtain A feed;
(2) preparation of B feed: first the brown shorts of B feedstuff composition, corn flour, flour, fish meal are put into disinfection cabinet and heat, temperature is sterilized at keeping 60-90 ℃ 4-5 hour, kill germ, insect, evil mite and ovum thereof in feed, again brown shorts and water are fully mixed thoroughly, then added corn flour, flour, fish meal fully to mix thoroughly again and obtain B feed together with dusty yeast.
A kind of method of indoor propagation Trichogramma pintoi, in mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, to be returned indoor collection Trichogramma pintoi as honeybee kind provenance by the ovum collection of Trichogramma, breed in a large number as vector using rice moth egg indoor, propagation method comprises the following steps:
(1) breeding of rice moth: when rice moth inoculation, at the thick A feed of plastic crate middle berth one deck 1.1-1.2 cm, on feed, evenly spill rice moth kind ovum, free-and-easy one deck just covers the flour bisque of ovum grain again, is placed in rice moth and raises indoor feeding, and the larva after hatching starts feeding after eating out thickly dotted aperture, within first 15 days, throw in A feed in inoculation, throw in afterwards B feed, within every 3 days, throw a defective material, input amount is that the larva appetite of 3 days is until larvae pupation; Pupa is collected adult after sprouting wings and is placed in the cage of laying eggs and lays eggs, and collects rice moth egg every day, and part is directly used in the breeding of rice moth, and remainder kills and performs mark after embryo and put into the refrigerator of 3-5 ℃ and preserve with ultraviolet light, is made into rice moth egg card before numerous honeybee, for subsequent use;
(2) field bee kind: in mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, gather by the ovum of Trichogramma, return indoor bottle and collect Trichogramma pintoi as honeybee kind provenance, in the bottle of collecting honeybee kind, access rice moth egg card, parasitism is bred into oophagous trichogrammae breeding kind of honeybee card, for subsequent use;
(3) breeding of oophagous trichogrammae: 1 oophagous trichogrammae breeding kind of honeybee card is put into breeding bee tube numerous honeybee is indoor, clog the mouth of pipe with cotton plug, on honeybee card, snap into and in breeding bee tube, allow Trichogramma at 8 rice moth eggs of transfer room access when the bee eclosion of 60-70 %, put into the filter paper bar nursing of sweeping 10 % hydromels simultaneously, when access ovum card, the other end of the mouth of pipe is to light, and anti-kind of honeybee escapes from, after having inoculated, all breeding bee tubes that accessed ovum card are being palletized and covering shading with black cloth, then move into that numerous honeybee is indoor to lay eggs, after 8-14 hour, in transfer room, change to 8 new rice moth egg cards with same inoculation method, every breeding kind of honeybee card can connect 16 rice moth egg cards, postvaccinal rice moth egg card is standing and is being put in capsule and moves into that numerous honeybee is indoor continues to breed 4-5 days, then the refrigerator of putting into 3-5 ℃ carries out short-term preservation, during with honeybee, shift to an earlier date 4-7 days and take out and be placed in the indoor cultivation of numerous honeybee from refrigerator, the available expansion of honeybee card after cultivation is numerous or for biological control of insect pests.
Described rice moth receptacle control indoor temperature is 25-28 ℃, and relative moisture is 60-85 %.
It is 25-28 ℃ that indoor temperature is controlled in described numerous honeybee chamber, and relative moisture is 70-85 %.
Described breeding bee tube is the Boiling tube of diameter 30 mm × length 20 cm.
Described rice moth egg card is that the sticky rice moth egg of the strip label card of 2 cm × 4 cm is made.
Advantage of the present invention and beneficial effect have:
(1) Trichogramma pintoi that the present invention breeds is the extensive natural enemy insect of a class host, can be used in the biological control of multiple agriculture and forestry injurious insect.
(2) in rice moth of the present invention breeding, in larvae feeds, added fish meal, dusty yeast makes rice moth adult eclosion more neat, egg laying amount is higher.
(3) make the parasitic rate of oophagous trichogrammae higher with the numerous honeybee of Boiling tube, mean parasitized rate reaches more than 90%.
(4) the present invention is without large-scale instrument, and propagating materials used is easily obtained, simple to operate, practical.
(5) the present invention is environmentally friendly, pollution-free, is suitable for application and promotes, and has good social benefit.
Embodiment
Below in conjunction with specific embodiment, the present invention is further described.
embodiment 1:
One, the rice moth culturing feed of breeding Trichogramma pintoi:
Raw material composition and the parts by weight of A feed:
5 parts, 50 parts of brown shorts, 40 parts of corn flour, 10 parts, flour and water;
Raw material composition and the parts by weight of B feed:
5 parts, 50 parts of brown shorts, 40 parts of corn flour, 5 parts, flour, 3 parts of fish meal, 2 parts of dusty yeasts and water.
Described A feed and the preparation method of B feed be respectively:
(1) preparation of A feed: first the brown shorts of A feedstuff composition, corn flour, flour are put into disinfection cabinet and heat, temperature is sterilized 5 hours at keeping 60 ℃, kill germ, insect, evil mite and ovum thereof in feed, again brown shorts and water are fully mixed thoroughly, then added corn flour and flour fully to mix thoroughly again and obtain A feed;
(2) preparation of B feed: first the brown shorts of B feedstuff composition, corn flour, flour, fish meal are put into disinfection cabinet and heat, temperature is sterilized 5 hours at keeping 60 ℃, kill germ, insect, evil mite and ovum thereof in feed, again brown shorts and water are fully mixed thoroughly, then added corn flour, flour, fish meal fully to mix thoroughly again and obtain B feed together with dusty yeast.
Two, the method for indoor propagation Trichogramma pintoi:
In mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, using being returned indoor collection Trichogramma pintoi as honeybee kind provenance by the ovum collection of Trichogramma, to breed in a large number as vector using rice moth egg indoor, propagation method comprises the following steps:
(1) breeding of rice moth: when rice moth inoculation, at the thick A feed of plastic crate middle berth one deck 1.1cm, on feed, evenly spill rice moth kind ovum, free-and-easy one deck just covers the flour bisque of ovum grain again, be placed in rice moth and raise indoor feeding, controlling indoor temperature is 25 ℃, relative moisture is 70 %, larva after hatching starts feeding after eating out thickly dotted aperture, within first 15 days, throw in A feed in inoculation, throw in afterwards B feed, within every 3 days, throw a defective material, input amount is that the larva appetite of 3 days is until larvae pupation; The rear collection of pupa emergence adult is placed in the cage of laying eggs and lays eggs, collect rice moth egg every day, part is directly used in the breeding of rice moth, remainder kills and performs mark after embryo and put into the refrigerator of 3 ℃ and preserve with ultraviolet light, the sticky rice moth egg of strip label card with 2 cm × 4 cm before numerous honeybee is made into rice moth egg card, for subsequent use;
(3) field bee kind: in mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, gather by the ovum of Trichogramma, return indoor bottle and collect Trichogramma pintoi as honeybee kind provenance, in the bottle of collecting honeybee kind, access rice moth egg card, parasitism is bred into oophagous trichogrammae breeding kind of honeybee card, for subsequent use;
(4) breeding of oophagous trichogrammae: at the indoor breeding bee tube of 1 oophagous trichogrammae breeding kind of honeybee card being put into diameter 30mm × long 20cm of numerous honeybee, clog the mouth of pipe with cotton plug, on honeybee card, snap into and in breeding bee tube, allow Trichogramma at 8 rice moth eggs of transfer room access when 60% bee eclosion, put into the filter paper bar nursing of sweeping 10 % hydromels simultaneously, when access ovum card, the other end of the mouth of pipe is to light, and anti-kind of honeybee escapes from, after having inoculated, all breeding bee tubes that accessed ovum card are being palletized and covering shading with black cloth, then move into that numerous honeybee is indoor to lay eggs, controlling indoor temperature is 25 ℃, relative moisture is 70 %, after 8 hours, in transfer room, change to 8 new rice moth egg cards with same inoculation method, every breeding kind of honeybee card can connect 16 rice moth egg cards, postvaccinal rice moth egg card is standing and is being put in capsule and moves into that numerous honeybee is indoor to be continued to breed 4 days, mean parasitized rate reaches 90.6%, then the refrigerator of parasitic rice moth egg card being put into 3 ℃ carries out short-term preservation, during with honeybee, within 7 days, take out and be placed in the indoor cultivation of numerous honeybee from refrigerator in advance, the available expansion of honeybee card after cultivation is numerous or for biological control of insect pests.
embodiment 2:
One, the rice moth culturing feed of breeding Trichogramma pintoi:
Raw material composition and the parts by weight of A feed:
5 parts, 50 parts of brown shorts, 40 parts of corn flour, 10 parts, flour and water;
Raw material composition and the parts by weight of B feed:
5 parts, 50 parts of brown shorts, 40 parts of corn flour, 5 parts, flour, 3 parts of fish meal, 2 parts of dusty yeasts and water.
Described A feed and the preparation method of B feed be respectively:
(1) preparation of A feed: first the brown shorts of A feedstuff composition, corn flour, flour are put into disinfection cabinet and heat, temperature is sterilized 5 hours at keeping 70 ℃, kill germ, insect, evil mite and ovum thereof in feed, again brown shorts and water are fully mixed thoroughly, then added corn flour and flour fully to mix thoroughly again and obtain A feed;
(2) preparation of B feed: first the brown shorts of B feedstuff composition, corn flour, flour, fish meal are put into disinfection cabinet and heat, temperature is sterilized 5 hours at keeping 70 ℃, kill germ, insect, evil mite and ovum thereof in feed, again brown shorts and water are fully mixed thoroughly, then added corn flour, flour, fish meal fully to mix thoroughly again and obtain B feed together with dusty yeast.
Two, the method for indoor propagation Trichogramma pintoi:
In mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, using being returned indoor collection Trichogramma pintoi as honeybee kind provenance by the ovum collection of Trichogramma, to breed in a large number as vector using rice moth egg indoor, propagation method comprises the following steps:
(1) breeding of rice moth: when rice moth inoculation, at the thick A feed of plastic crate middle berth one deck 1.1cm, on feed, evenly spill rice moth kind ovum, free-and-easy one deck just covers the flour bisque of ovum grain again, be placed in rice moth and raise indoor feeding, controlling indoor temperature is 26 ℃, relative moisture is 75 %, larva after hatching starts feeding after eating out thickly dotted aperture, within first 15 days, throw in A feed in inoculation, throw in afterwards B feed, within every 3 days, throw a defective material, input amount is that the larva appetite of 3 days is until larvae pupation; The rear collection of pupa emergence adult is placed in the cage of laying eggs and lays eggs, collect rice moth egg every day, part is directly used in the breeding of rice moth, remainder kills and performs mark after embryo and put into the refrigerator of 4 ℃ and preserve with ultraviolet light, the sticky rice moth egg of strip label card with 2 cm × 4 cm before numerous honeybee is made into rice moth egg card, for subsequent use;
(3) field bee kind: in mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, gather by the ovum of Trichogramma, return indoor bottle and collect Trichogramma pintoi as honeybee kind provenance, in the bottle of collecting honeybee kind, access rice moth egg card, parasitism is bred into oophagous trichogrammae breeding kind of honeybee card, for subsequent use;
(4) breeding of oophagous trichogrammae: at the indoor breeding bee tube of 1 oophagous trichogrammae breeding kind of honeybee card being put into diameter 30mm × long 20cm of numerous honeybee, clog the mouth of pipe with cotton plug, on honeybee card, snap into and in breeding bee tube, allow Trichogramma at 8 rice moth eggs of transfer room access when the bee eclosion of 65 %, put into the filter paper bar nursing of sweeping 10 % hydromels simultaneously, when access ovum card, the other end of the mouth of pipe is to light, and anti-kind of honeybee escapes from, after having inoculated, all breeding bee tubes that accessed ovum card are being palletized and covering shading with black cloth, then move into that numerous honeybee is indoor to lay eggs, controlling indoor temperature is 26 ℃, relative moisture is 75 %, after 10 hours, in transfer room, change to 8 new rice moth egg cards with same inoculation method, every breeding kind of honeybee card can connect 16 rice moth egg cards, postvaccinal rice moth egg card is standing and is being put in capsule and moves into that numerous honeybee is indoor to be continued to breed 5 days, mean parasitized rate reaches 92.1%, then the refrigerator of parasitic rice moth egg card being put into 4 ℃ carries out short-term preservation, during with honeybee, within 5 days, take out and be placed in the indoor cultivation of numerous honeybee from refrigerator in advance, the available expansion of honeybee card after cultivation is numerous or for biological control of insect pests.
embodiment 3:
One, the rice moth culturing feed of breeding Trichogramma pintoi:
Raw material composition and the parts by weight of A feed:
5 parts, 50 parts of brown shorts, 40 parts of corn flour, 10 parts, flour and water;
Raw material composition and the parts by weight of B feed:
5 parts, 50 parts of brown shorts, 40 parts of corn flour, 5 parts, flour, 3 parts of fish meal, 2 parts of dusty yeasts and water.
Described A feed and the preparation method of B feed be respectively:
(1) preparation of A feed: first the brown shorts of A feedstuff composition, corn flour, flour are put into disinfection cabinet and heat, temperature is sterilized 4 hours at keeping 90 ℃, kill germ, insect, evil mite and ovum thereof in feed, again brown shorts and water are fully mixed thoroughly, then added corn flour and flour fully to mix thoroughly again and obtain A feed;
(2) preparation of B feed: first the brown shorts of B feedstuff composition, corn flour, flour, fish meal are put into disinfection cabinet and heat, temperature is sterilized 4 hours at keeping 90 ℃, kill germ, insect, evil mite and ovum thereof in feed, again brown shorts and water are fully mixed thoroughly, then added corn flour, flour, fish meal fully to mix thoroughly again and obtain B feed together with dusty yeast.
Two, the method for indoor propagation Trichogramma pintoi:
In mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, using being returned indoor collection Trichogramma pintoi as honeybee kind provenance by the ovum collection of Trichogramma, to breed in a large number as vector using rice moth egg indoor, propagation method comprises the following steps:
(1) breeding of rice moth: when rice moth inoculation, at the thick A feed of plastic crate middle berth one deck 1.2 cm, on feed, evenly spill rice moth kind ovum, free-and-easy one deck just covers the flour bisque of ovum grain again, be placed in rice moth and raise indoor feeding, controlling indoor temperature is 28 ℃, relative moisture is 80 %, larva after hatching starts feeding after eating out thickly dotted aperture, within first 15 days, throw in A feed in inoculation, throw in afterwards B feed, within every 3 days, throw a defective material, input amount is that the larva appetite of 3 days is until larvae pupation; The rear collection of pupa emergence adult is placed in the cage of laying eggs and lays eggs, collect rice moth egg every day, part is directly used in the breeding of rice moth, remainder kills and performs mark after embryo and put into the refrigerator of 5 ℃ and preserve with ultraviolet light, the sticky rice moth egg of strip label card with 2 cm × 4 cm before numerous honeybee is made into rice moth egg card, for subsequent use;
(3) field bee kind: in mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, gather by the ovum of Trichogramma, return indoor bottle and collect Trichogramma pintoi as honeybee kind provenance, in the bottle of collecting honeybee kind, access rice moth egg card, parasitism is bred into oophagous trichogrammae breeding kind of honeybee card, for subsequent use;
(4) breeding of oophagous trichogrammae: at the indoor breeding bee tube of 1 oophagous trichogrammae breeding kind of honeybee card being put into diameter 30mm × long 20cm of numerous honeybee, clog the mouth of pipe with cotton plug, on honeybee card, snap into and in breeding bee tube, allow Trichogramma at 8 rice moth eggs of transfer room access when 70% bee eclosion, put into the filter paper bar nursing of sweeping 10 % hydromels simultaneously, when access ovum card, the other end of the mouth of pipe is to light, and anti-kind of honeybee escapes from, after having inoculated, all breeding bee tubes that accessed ovum card are being palletized and covering shading with black cloth, then move into that numerous honeybee is indoor to lay eggs, controlling indoor temperature is 28 ℃, relative moisture is 80 %, after 12 hours, in transfer room, change to 8 new rice moth egg cards with same inoculation method, every breeding kind of honeybee card can connect 16 rice moth egg cards, postvaccinal rice moth egg card is standing and is being put in capsule and moves into that numerous honeybee is indoor to be continued to breed 4 days, mean parasitized rate reaches 91.3%, then the refrigerator of parasitic rice moth egg card being put into 5 ℃ carries out short-term preservation, during with honeybee, within 6 days, take out and be placed in the indoor cultivation of numerous honeybee from refrigerator in advance, the available expansion of honeybee card after cultivation is numerous or for biological control of insect pests.