CN104756960B - Large-speckle small yellow bee breeding method - Google Patents

Abstract

The invention discloses a method for raising A. scutellariae, which comprises the following steps: collecting healthy larvae of Leafroller larvae from a paddy field, raising them indoors until they become adults, or directly collecting adults from the field; allowing the male and female adults to mate, Transfer the mated females to the rice seedlings placed in insect cages to lay eggs, and directly feed on the rice leaves after the eggs hatch; the rice leaf rollers larvae collected from the field and parasitized by the yellow wasp or Put the pupae of the wasp into the finger tube and bring it back to the laboratory, and feed it with honey water after the adults of the yellow wasp emerge; pair the male and female adults of the yellow wasp, and then put the adult yellow wasp after mating The female wasps were transferred to the insect cages with rice seedlings and rice leaf roller larvae, and let them freely parasitize and feed on the rice leaf roller larvae on the rice leaves. After the leaf roller larvae, the parasitic rice leaf roller larvae will be retracted into the finger tube, and kept moist and cultivated until the mature larvae of the rice leaf roller climb away from the rice leaf roller larvae; The cooked larvae are kept moist and cultivated until they pupate and emerge, and the eclosion adults of V. chinensis can be used to parasitize the larvae of the rice leaf roller; this method is suitable for artificially raising V. It has a high survival rate and does not carry other diseases or miscellaneous bacteria, providing guidance for the biological control of the rice leaf roller.

Description

Breeding method of large spotted yellow bee

technical field

The technology belongs to the field of biological feeding, specifically relates to a feeding method of the yellow wasp, and can be used in the field of biological control of pests.

Background technique

At present, the means of controlling the migratory pest rice leaf roller is mainly chemical control, but excessive and indiscriminate application of pesticides often lead to environmental pollution, killing of natural enemies, pest resistance and re-emergence. Most pesticide users ignore the existence of a large number of natural enemies of pests in the field, especially the existence of parasitic wasps. For example, the total parasitism rate of parasitoid wasps in Xinjiang in 1978 was between 33-90%. In the first half of the year, the highest parasitism rate of the rice leaf roller was 39.22% (Huang Xiuzhi et al., 2013). In the past three decades, many parasitic and predatory natural enemies have been identified, and more than 100 species of parasitoids have been identified in Asia (He and Chen 1990; Ma et al. 2002; Guizhou Agricultural science and technology Committee 1986; Lou et al. 2014). However, few of these parasitic wasps have been used to control rice leaf rollers in the field. They are only naturally occurring groups in the ecosystem and have not yet been specially used by humans for the control of rice leaf rollers. superior. The breeding of the rice leaf roller parasitoid is rarely touched. Therefore, studying a method of artificial breeding of dominant parasitoids of C. sativa may provide technical guidance for the biological control of C. sativa.

Contents of the invention

One of the purposes of the present invention is to solve the above-mentioned lack of a breeding method for the parasitic wasp of the leaf roller rice leaf roller - the yellow wasp, that is, to provide a method for artificially raising the yellow wasp.

A kind of method for raising yellow hornets, comprising the following steps:

(1) Collection of rice leaf roller: collect healthy rice leaf roller larvae from paddy fields, raise them indoors to adults, or directly collect rice leaf roller adults from the field;

(2) Raising rice leaf rollers: put the male and female adults into the insect cage to mate, transfer the mated females to the rice seedlings in the insect cage to lay eggs, and drive out the female adults after 3 days after laying eggs Let the eggs hatch freely on the leaves to become larvae; the rice leaf roller larvae directly eat the rice leaves, continue to grow and develop until they are mature, collect the mature rice leaf roller larvae into the finger tube, and wait for them to pupate; After the pupa emerges, directly release the adults of the rice leaf roller to the rice seedlings that have been placed in the insect cage to make them lay eggs, so that the population of the rice leaf roller can continue to reproduce indoors;

(3) Collecting V. cerevisiae: Put the larvae of Leafroller larvae or pupae of V. cerevisiae collected from the field and parasitized by C. A rubber plug with air holes on the tube plug; feed with 5%-10% honey water after the adults of the yellow wasp emerge;

(4) Breeding of the yellow wasp: use sterilized absorbent cotton dipped in 5%-10% honey water to feed the adults of the yellow wasp, then make the adults of the yellow wasp paired with each other, and the female of the yellow wasp after mating The adults were transferred to insect cages with rice seedlings, and the rice seedlings in the insect cages were fed with 3rd instar rice leaf roller larvae, so that the female adults of B. , after 3 days of parasitism, the parasitic rice leaf roller larvae were retracted into the finger tube, kept moist and cultivated, and the rice leaf roller larvae were replaced with fresh rice leaves every day until the mature larvae of the rice leaf roller crawled away rice leaf roller larvae; collect the mature larvae of the rice leaf roller; collect the mature larvae of the rice leaf roller, keep them moisturized, and wait for them to pupate; Propagation of a generation of P. cerevisiae population;

During the feeding process of each stage of Leafroller oryzae and the feeding process of each stage of P. eczema, the indoor breeding temperature is set to be 25°C to 27°C, and the humidity is between 70% to 80%.

The beneficial effect of the present invention is that: the present invention provides a method suitable for artificially raising the yellow wasp, which has a high survival rate and does not carry other diseases or miscellaneous bacteria. The biological control of borer provides guidance.

detailed description

The content of the present invention will be described in detail below in conjunction with specific embodiments. It should be noted that the technical features or combinations of technical features described in the following embodiments should not be regarded as isolated, and they can be combined with each other to achieve better technical effects.

Example 1

1. Breeding utensils

1.1 insect cage

Large insect cage (length × width × height = 1m × 1m × 1.5m), with stainless steel as the support, five sides are gauze with good ventilation and light transmission, two doors in the front, and plastic partitions on the bottom. The material and structure of the small experimental cage (length * width * height = 30cm * 30cm * 50cm) are consistent with those of the large insect cage.

1.2 potted rice pot

The specification is a plastic barrel with a diameter of 20cm and a height of 25cm.

1.3 Other materials

Finger tubes, plastic stoppers, label paper, rubber bands, blackout cloth (black cloth), sterilized cotton, pins, honey mixture (diluted to 10%), trays, insect nets.

2. Plant rice

The rice variety is Yesizhan and it is potted. Select the rice seeds, remove the empty, deflated, miscellaneous, and rotten grains, spread the selected seeds on the tray, add water until the water surface just covers all the seeds, soak for a day or two until the seeds show white buds, and then After more than ten hours, when the buds are about 0.2 cm long, spread the germinated seeds in the seedling trays, cover the seeds with thin soil, and water until the soil is submerged. Make the seeds grow. After the seedlings grow to a height of 20 cm, the seedlings are transplanted into the potted pots, and then all the potted plants are put into insect cages. One week after transplanting, fertilize the rice. retain.

3. Collect and raise rice leaf roller

Choose a rice field that has not been sprayed with pesticides, collect healthy rice leaf roller larvae from it, use an insect net to sweep and catch rice leaf roller adults, and raise the collected larvae indoors until they become adults. The collected adults or indoor rearing The adults were directly put into small experimental cages to mate, and then the mated females were transferred to the planted rice seedlings to lay eggs freely. After 3 days, the adults were driven out, and the eggs were allowed to hatch freely on the leaves. The hatched rice leaf roller larvae are grown to the 3rd instar on the potted rice, and are kept for use, waiting to pick up the adults of the yellow wasp.

4. Collect and raise the large spotted yellow wasp

The larvae of the rice leaf roller and the pupae of the rice leaf roller parasitized by the yellow wasp were collected from the field, put into the finger tube, and plugged with a rubber plug with air holes (parasitized by the rice leaf roller The larvae need to be replaced with fresh rice leaves every day, and when the mature larvae of the larvae climb away from the larvae of the rice leaf roller, they pupate on the same day or the next day; the pupae of the collected larvae do not need to be fed on the rice leaves), etc. The pupae of the large spotted yellow wasp emerges as an adult, and is fed with 10% diluted honey water dipped in sterilized absorbent cotton.

Pair the male and female of the large spotted yellow wasp, and transfer the paired large spotted yellow wasp to the insect cage containing the rice seedlings that raise the 3rd instar rice leaf roller larvae, and let them naturally parasitize the rice on the rice. Leaf roller larvae, after being parasitized for 3 days, retract the rice leaf roller larvae parasitized by A. The mature larvae of the yellow wasp crawled away from the larvae of the rice leaf roller. Collect the mature larvae of the wasps and wait for them to pupate.

The eclosion's adult wasp continues to let it parasitize the 3rd instar larvae of the rice leaf roller, thus starting the reproduction of the next generation of wasp. In the adult stage of the large spotted yellow wasp, 5%-10% honey water should be fed in time and placed on the rice seedlings with the 3rd instar larvae of the rice leaf roller.

5. Experiment and results of parasitism rate of different instar larvae of rice leaf roller

Place 15 pots of healthy potted rice in each insect cage, and every three pots is a group, so that each group of rice does not contact each other. Use label paper to number each group of rice pots as 1, 2, 3, 4, and 5, and connect the reared rice leaf rollers of each instar larvae to the corresponding numbered rice leaves according to the order of each instar (ie: 1 No. 1 is the first instar larva, No. 2 is the second instar larva, No. 3 is the third instar larva, No. 4 is the fourth instar larva, and No. 5 is the fifth instar larva). Each cage is a replicate, a total of three replicates. Put 15 rice leaf roller larvae on every three pots of rice, and put 10 wasps per cage (the ratio of male to female is about 1:3). Close the cage door to prevent the large spotted yellow bee from running out and other insects from interfering with the experiment, while keeping the rice growth ventilated and light-transmitting. After 3 days, the insect cage was opened to drive out the large spotted yellow wasp, and the larvae of all instars of the rice leaf roller participating in the experiment were collected back to the room one by one, and they were fed with rice leaves in the finger tube, and fresh rice was replaced every day. Leaves, keep room temperature 25-27 ℃, keep 75-80% humidity in the finger tube. The test results showed that the parasitism rate of the larvae of Leafroller larvae of different ages by the large spotted yellow wasp: the parasitism rate of the second instar was 16.67%, the parasitization rate of the third instar was 30.23%, the parasitism rate of the fourth instar was 15.56%, and the parasitism rate of the fifth instar was 16.67%. The rate was 2.78%, and no data were found that the first instar larvae developed to pupate or gave birth to V. The adult parasitism rate of the 3rd-instar Leafroller larvae was the highest. Under suitable temperature and humidity, the ratio of the successful parasitization to the normal pupation of the mature larvae from the host to the normal pupation of the wasp is very high, and both pupation and eclosion can be normal.

Although some embodiments of the present invention have been given herein, those skilled in the art should understand that the embodiments herein can be changed without departing from the spirit of the present invention. The above-mentioned embodiments are only exemplary, and the embodiments herein should not be used as limitations on the scope of rights of the present invention.

Claims (1)

1. a method for raising a large spotted yellow bee, is characterized in that, comprises the following steps: (1) Collection of rice leaf roller: collect healthy rice leaf roller larvae from paddy fields, raise them indoors to adults, or directly collect rice leaf roller adults from the field; (2) Raising rice leaf rollers: put the male and female adults into the insect cage to mate, transfer the mated females to the rice seedlings in the insect cage to lay eggs, and drive out the female adults after 3 days after laying eggs Let the eggs hatch freely on the leaves to become larvae; the rice leaf roller larvae directly eat the rice leaves, continue to grow and develop until they are mature, collect the mature rice leaf roller larvae into the finger tube, and wait for them to pupate; After the pupa emerges, directly release the adults of the rice leaf roller to the rice seedlings that have been placed in the insect cage to make them lay eggs, so that the population of the rice leaf roller can continue to reproduce indoors; (3) Collecting V. cerevisiae: Put the larvae of Leafroller larvae or pupae of V. cerevisiae collected from the field and parasitized by C. A rubber plug with air holes on the tube plug; feed with 5%-10% honey water after the adults of the yellow wasp emerge; (4) Breeding of the wasp: use sterilized absorbent cotton dipped in 5%-10% honey water to feed the adults of the wasp, and then pair the adults of the wasp with males and females, and the females of the wasp after mating The adults were transferred to the insect cages with the rice seedlings, and the rice seedlings in the insect cages were raised with the 3rd instar larvae of the rice leaf roller, so that the female adults of the wasp naturally parasitized the 3rd instar larvae of the rice leaf roller , after 3 days of parasitism, the parasitic rice leaf roller larvae were retracted into the finger tube, kept moist and cultivated, and the rice leaf roller larvae were replaced with fresh rice leaves every day until the mature larvae of the rice leaf roller crawled away rice leaf roller larvae; collect the mature larvae of the rice leaf roller; collect the mature larvae of the rice leaf roller, keep them moisturized, and wait for them to pupate; Breeding of a generation of large spotted yellow wasps; During the feeding process of each stage of Leafroller oryzae and the feeding process of each stage of P. eczema, the indoor breeding temperature is set to be 25°C to 27°C, and the humidity is between 70% to 80%.