The indoor feeding method of a kind of bright red hair spot moth and device
Technical field
The present invention relates to raising method and the raising device thereof of insect, specifically a kind of bright red hair spot moth indoor feeding method and put.
Background technology
Bright red hair spot moth
phaudaflammanswalker(Lepidoptera Lepidoptera: Leaf skeletonizer moth Zygaenidae) be a kind of defoliator on banyan.In recent years, in the tropical country such as India, Vietnam, Burma, Malaysia, Philippine and China Guangxi, Guangdong, Hainan etc., southern provinces and regions occur and cause harm in outburst trend, by minor pests rise to primary pest (Liu Junyan etc. the investigation method of banyan defoliator bright red hair spot chrysalis. Chinese plant protection guide, 2014).Be injured the whole strain blades such as serious garden plants, street tree, hedgerow all by food light, and only surplus limb, has a strong impact on urban look, image and ecological benefits.
Research shows, the main affecting factors affecting insect population outburst is host, ecotope (as shortage effective natural enemies) that worm source radix (comprise and survive the winter and get over summer radix) and favourable population quantity increase (Meng Qingfan. the progress of forest vegetation diversity and pest population management. World Forestry studies 2003).First, species richness and many angle value of landscape ecology system are low, and the plantation of the single seeds of large area provides abundant foodstuff to pest population and impels its amount reproduction, is the one of the main reasons causing its population to be broken out.Because in landscape ecology system, the seeds of greening are limited, and the large area of single seeds is planted and provided abundant foodstuff to insect, contributes to the Fast-propagation of insect (especially phytophagy kind) population.Secondly, local weather conditions can be adapted to and form the basis that certain summer radix of surviving the winter is pest population outburst.Be mainly reflected in the tolerance (comprise the duration of limit high/low temperature and high/low temperature) of pest population to local limit high/low temperature, tolerance is strong, and survival rate is high, and population radix is high.Finally, because the species diversity of landscape ecology system is lower, diversified habitat and abundant food source etc. can not be formed and can hold more caste, natural enemy, predacious arthropod, insectivorous birds and other animal species, do not regulate by the species diversity in its own system or resist causing harm of insect.Seeds selected by urban afforestation space and artificial greening are limited, narrow, the impact of managing and protecting the factors such as the plant nutrition being not good at causing is bad of the artificial disturbance underground space that is serious, arboreal growth in city, be difficult to build the higher garden landscape of species richness, cause urban green space system to be difficult to set up the ecological functions of stable equilibrium.So that can not attract more to enrich Natural Enemies, thus make pest population lack the suppression of effective natural enemies to cause its population explosive growth, have this scholar propose natural enemy hypothesis (Naturalenemyhypothesis) (Meng Qingfan. the progress of forest vegetation diversity and pest population management. World Forestry studies 2003).Therefore, grasping the impact on bright red hair spot moth population existence and breeding of main and crucial biology (as host, natural enemy) and abiotic factor (temperature), is resolve the prerequisite of its population outburst and basic.
But, understand fully that biology (as host, natural enemy) and the impact of abiotic factor (temperature) on bright red hair spot moth population existence and breeding but need a large amount of, that worm state development progress is more consistent indoor population as supplying examination worm source.At present, yet there are no the report of the technology of bright red hair spot moth indoor feeding both at home and abroad.Given this, work out a set of bright red hair spot moth indoors artificial breeding technology, to realize the continuously rearing trying the long-term scale of worm, thus providing sufficient worm source for probing into its outburst mechanism, just seeming particularly important.
Summary of the invention
The object of this invention is to provide indoor feeding method and the device of a kind of bright red hair spot moth, it is large that this raising method and device can raise out quantity, and the bright red hair spot moth that worm state development progress is more consistent, as biological (as host, natural enemy) and abiotic factor (temperature) on bright red hair spot moth population existence and the worm source of the research of impact of breeding.
To achieve these goals, the technical solution used in the present invention is:
The indoor feeding method of bright red hair spot moth, it comprises the raising step of larva, pupa, adult and ovum, and specifically bright red hair spot moth newly hatched larvae is raised in larva raising device, is foodstuff, until it is pupated with Ficus microcarpa; Male and female pupa is separated in square plastic magazine after differentiating, after sprouting wings, pairing is placed in adult mating device; The pairing of the adult of post-coitum is placed in Adult worms producting eggs device and treats that it is laid eggs; Collect the filter paper producing in Adult worms producting eggs device and have ovum grain, put into the clean square plastic magazine that bottom is covered with moisturizing filter paper, be placed in climatic cabinate, every day notes moisturizing, to its hatching; After larvae hatch, larva is carried out continuously rearing according to above-mentioned step cycle.
Newly hatched larvae to the detailed process of pupating is: using Ficus microcarpa as foodstuff, and be placed on the filter in larva raising device, filter can be used as the platform and isolation larva, foodstuff and excreta of putting foodstuff, avoid polypide and foodstuff to be subject to excremental pollution, and finally cause larva Infective microbial death.With sterile writing brush picking newly hatched larvae on foodstuff, be placed in climatic cabinate after building plastic box cover and raise; Temperature in described climatic cabinate is 25 DEG C-30 DEG C, the photoperiod is 14L:10D and humidity is 60% ± 10%; Within every two days, change a foodstuff, pick up filter simultaneously, the excreta of cleaning plastic box bottom, raising to cocoon to adult at box inwall pupates.
Pupate to sprout wings detailed process be: with sterile tweezers, cocoon is taken out from larva raising device, after strip off cocoon, distinguishes male and female pupa; Respectively male and female pupa is put into two square plastic magazines of having sterilized, place wet cotton balls moisturizing in box, and square plastic magazine is placed in climatic cabinate, until it is sprouted wings; Temperature in described climatic cabinate is 25 DEG C-30 DEG C, the photoperiod is 14L:10D and humidity is 60% ± 10%.
Sprout wings to mating detailed process and be: the female male worm just sprouted wings is placed in adult mating device, lets alone pangamy, 2 dark after dates take out adults, and become to be opposite in Adult worms producting eggs device and lay eggs, and check in Adult worms producting eggs device whether filter paper has ovum grain every day; If any, the filter paper more renewed.
Hatching to larva detailed process is: collect the filter paper producing and have ovum grain, puts into the clean square plastic magazine that bottom is covered with moisturizing filter paper, is placed in climatic cabinate, to its hatching; Temperature in described climatic cabinate is 25 DEG C-30 DEG C, the photoperiod is 14L:10D and humidity is 60% ± 10%, and the larva after hatching continues to carry out continuously rearing according to above-mentioned steps circulation; For studying the worm source that the impact on bright red hair spot moth population existence and breeding of biology (as host, natural enemy) and abiotic factor (temperature) provides worm state development progress more consistent.
Described larva raising device, comprise: the square box that the mouth the be made of plastics wide end is narrow and lid, lid is placed on square box, lid has box cover hole, box cover hole is provided with cap bore net, ensure there is no gap between cap bore net and lid, so not only can increase the permeability of whole plastic casing but also larva escape can be prevented; In square box, middle and lower part is provided with filter.
Described filter is made up of stainless steel, and its width of mesh is 10 orders.
Described cap bore net is gauze, and its width of mesh is 10-15 order.
Described adult mating device, comprise gauze and the framework in Fang Tizhuan, gauze is arranged on six of the framework in Fang Tizhuan, net wherein on a frame is can switch, be convenient to put into and take out adult, be hung with in adult mating device and the dactylethrae that weight content is the hydromel of 10% is housed, an aperture is bored in dactylethrae lid, strip absorbent cotton is through this hole, staying long outside dactylethrae lid is 0.5cm-1cm, remaining strip absorbent cotton is all filled in dactylethrae, by the function of the self-service water suction of degreasing sliver, make the degreasing sliver outside pipe moistening for a long time, supply Adult supplement nutrient.The width of mesh of described gauze is 25-30 order.Weight content be 10% hydromel refer to and get honey and cold water or clear water by weight, its Homogeneous phase mixing is obtained.
Described Adult worms producting eggs device, comprise culture dish, vermiculite and disposal plastic cup, vermiculite is placed in culture dish, vermiculite is coated with filter paper, disposal plastic cup back-off covers vermiculite, offer through hole bottom described disposal plastic cup, through hole is that the cotton balls plug of the hydromel of 10% blocks up by being moistened with weight content.
Outstanding substantive distinguishing features of the present invention and significant progress are:
1, the present invention worm source that worm state development progress can be provided more consistent on bright red hair spot moth population existence and the impact of breeding for research biological (as host, natural enemy) and abiotic factor (temperature).And the raising device simple structure related to, with low cost, operate simple and easy, recycle.
2, the square plastic magazine adopting the mouth wide end narrow in the present invention is as the raising device of bright red hair spot moth larvae, square stainless steel cloth is embedded in the middle and lower part in square plastic magazine, this device can be used as the platform putting foodstuff, larva, foodstuff and excreta can also be isolated simultaneously, avoid polypide and foodstuff to be subject to excremental pollution, solve in lepidopterous larvae indoor feeding because polypide contacts the problem of excreta and Infective microbial death.
3, adopt dactylethrae to provide the method for hydromel in imago breeding method of the present invention, hydromel can be avoided to be polluted, nutrition can also be provided to adult incessantly for a long time, both extend the life-span of adult, turn improve the egg laying amount of female worm.In addition, adopt the plastic cup method of laying eggs, be conducive to collecting the ovum grain/block just produced in time, avoid ovum grain/block to produce in the place of the not easily collecting such as adult mating cage inwall.This device is also applicable to the imago breeding link of other lepidopterous insects such as Noctuidae, Pyralidae.
Accompanying drawing explanation
Fig. 1 is the structural representation of bright red hair spot moth larvae raising device of the present invention;
Fig. 2 is the structural representation of bright red hair spot moth adult mating device of the present invention;
Fig. 3 is the structural representation of bright red hair spot moth Adult worms producting eggs device of the present invention;
Title in figure and sequence number are:
Square box 1, lid 2, box cover hole 3, cap bore net 4, filter 5, gauze 6, framework 7, strip absorbent cotton 8, dactylethrae 9, culture dish 10, vermiculite 11, disposal plastic cup 12, through hole 13, cotton balls 14.
Embodiment
Introduce the present invention in order to more detailed, below in conjunction with drawings and Examples, the present invention will be further described.
Embodiment 1
The indoor feeding method of bright red hair spot moth, it comprises the raising step of larva, pupa, adult and ovum, and specifically bright red hair spot moth newly hatched larvae is raised in larva raising device, is foodstuff, until it is pupated with Ficus microcarpa; Male and female pupa is separated in square plastic magazine after differentiating, after sprouting wings, pairing is placed in adult mating device; The pairing of the adult of post-coitum is placed in Adult worms producting eggs device and treats that it is laid eggs; Collect the filter paper producing in Adult worms producting eggs device and have ovum grain, put into the clean square plastic magazine that bottom is covered with moisturizing filter paper, be placed in climatic cabinate, every day notes moisturizing, to its hatching; After larvae hatch, larva is carried out continuously rearing according to above-mentioned step cycle.
Newly hatched larvae to the detailed process of pupating is: using Ficus microcarpa as foodstuff, and be placed on the filter in larva raising device, filter can be used as the platform and isolation larva, foodstuff and excreta of putting foodstuff, avoid polypide and foodstuff to be subject to excremental pollution, and finally cause larva Infective microbial death.With sterile writing brush picking newly hatched larvae on foodstuff, be placed in climatic cabinate after building plastic box cover and raise; Temperature in described climatic cabinate is 25 DEG C, the photoperiod is 14L:10D and humidity is 60% ± 10%; Within every two days, change a foodstuff, pick up filter simultaneously, the excreta of cleaning plastic box bottom, pupates until larva cocoons at box inwall.
Pupate to sprout wings detailed process be: with sterile tweezers, cocoon is taken out from larva raising device, after strip off cocoon, distinguishes male and female pupa; Respectively male and female pupa is put into two square plastic magazines of having sterilized, place wet cotton balls moisturizing in box, and square plastic magazine is placed in climatic cabinate, until it is sprouted wings; Temperature in described climatic cabinate is 25 DEG C, the photoperiod is 14L:10D and humidity is 60% ± 10%.
Sprout wings to mating detailed process and be: the female male worm just sprouted wings is placed in adult mating device, lets alone pangamy, 2 dark after dates take out adults, and become to be opposite in Adult worms producting eggs device and lay eggs, and check in Adult worms producting eggs device whether filter paper has ovum grain every day; If any, the filter paper more renewed.
Hatching to larva detailed process is: collect the filter paper producing and have ovum grain, puts into the clean square plastic magazine that bottom is covered with moisturizing filter paper, is placed in climatic cabinate, to its hatching; Temperature in described climatic cabinate is 25 DEG C, the photoperiod is 14L:10D and humidity is 60% ± 10%, and the larva after hatching continues to carry out continuously rearing according to above-mentioned steps circulation; For studying the worm source that biological and abiotic factor provides worm state development progress more consistent on bright red hair spot moth population existence and the impact of breeding.
Embodiment 2
The indoor feeding method of bright red hair spot moth, it comprises the raising step of larva, pupa, adult and ovum, and specifically bright red hair spot moth newly hatched larvae is raised in larva raising device, is foodstuff, until it is pupated with Ficus microcarpa; Male and female pupa is separated in square plastic magazine after differentiating, after sprouting wings, pairing is placed in adult mating device; The pairing of the adult of post-coitum is placed in Adult worms producting eggs device and treats that it is laid eggs; Collect the filter paper producing in Adult worms producting eggs device and have ovum grain, put into the clean square plastic magazine that bottom is covered with moisturizing filter paper, be placed in climatic cabinate, every day notes moisturizing, to its hatching; After larvae hatch, larva is carried out continuously rearing according to above-mentioned step cycle.
Newly hatched larvae to the detailed process of pupating is: using Ficus microcarpa as foodstuff, and be placed on the filter in larva raising device, filter can be used as the platform and isolation larva, foodstuff and excreta of putting foodstuff, avoid polypide and foodstuff to be subject to excremental pollution, and finally cause larva Infective microbial death.With sterile writing brush picking newly hatched larvae on foodstuff, be placed in climatic cabinate after building plastic box cover and raise; Temperature in described climatic cabinate is 26 DEG C, the photoperiod is 14L:10D and humidity is 60% ± 10%; Within every two days, change a foodstuff, pick up filter simultaneously, the excreta of cleaning plastic box bottom, pupates until larva cocoons at box inwall.
Pupate to sprout wings detailed process be: with sterile tweezers, cocoon is taken out from larva raising device, after strip off cocoon, distinguishes male and female pupa; Respectively male and female pupa is put into two square plastic magazines of having sterilized, place wet cotton balls moisturizing in box, and square plastic magazine is placed in climatic cabinate, until it is sprouted wings; Temperature in described climatic cabinate is 26 DEG C, the photoperiod is 14L:10D and humidity is 60% ± 10%.
Sprout wings to mating detailed process and be: the female male worm just sprouted wings is placed in adult mating device, lets alone pangamy, 2 dark after dates take out adults, and become to be opposite in Adult worms producting eggs device and lay eggs, and check in Adult worms producting eggs device whether filter paper has ovum grain every day; If any, the filter paper more renewed.
Hatching to larva detailed process is: collect the filter paper producing and have ovum grain, puts into the clean square plastic magazine that bottom is covered with moisturizing filter paper, is placed in climatic cabinate, to its hatching; Temperature in described climatic cabinate is 26 DEG C, the photoperiod is 14L:10D and humidity is 60% ± 10%, and the larva after hatching continues to carry out continuously rearing according to above-mentioned steps circulation; For studying the worm source that biological and abiotic factor provides worm state development progress more consistent on bright red hair spot moth population existence and the impact of breeding.
Embodiment 3
The indoor feeding method of bright red hair spot moth, it comprises the raising step of larva, pupa, adult and ovum, and specifically bright red hair spot moth newly hatched larvae is raised in larva raising device, is foodstuff, until it is pupated with Ficus microcarpa; Male and female pupa is separated in square plastic magazine after differentiating, after sprouting wings, pairing is placed in adult mating device; The pairing of the adult of post-coitum is placed in Adult worms producting eggs device and treats that it is laid eggs; Collect the filter paper producing in Adult worms producting eggs device and have ovum grain, put into the clean square plastic magazine that bottom is covered with moisturizing filter paper, be placed in climatic cabinate, every day notes moisturizing, to its hatching; After larvae hatch, larva is carried out continuously rearing according to above-mentioned step cycle.
Newly hatched larvae to the detailed process of pupating is: using Ficus microcarpa as foodstuff, and be placed on the filter in larva raising device, filter can be used as the platform and isolation larva, foodstuff and excreta of putting foodstuff, avoid polypide and foodstuff to be subject to excremental pollution, and finally cause larva Infective microbial death.With sterile writing brush picking newly hatched larvae on foodstuff, be placed in climatic cabinate after building plastic box cover and raise; Temperature in described climatic cabinate is 27 DEG C, the photoperiod is 14L:10D and humidity is 60% ± 10%; Within every two days, change a foodstuff, pick up filter simultaneously, the excreta of cleaning plastic box bottom, pupates until larva cocoons at box inwall.
Pupate to sprout wings detailed process be: with sterile tweezers, cocoon is taken out from larva raising device, after strip off cocoon, distinguishes male and female pupa; Respectively male and female pupa is put into two square plastic magazines of having sterilized, place wet cotton balls moisturizing in box, and square plastic magazine is placed in climatic cabinate, until it is sprouted wings; Temperature in described climatic cabinate is 27 DEG C, the photoperiod is 14L:10D and humidity is 60% ± 10%.
Sprout wings to mating detailed process and be: the female male worm just sprouted wings is placed in adult mating device, lets alone pangamy, 2 dark after dates take out adults, and become to be opposite in Adult worms producting eggs device and lay eggs, and check in Adult worms producting eggs device whether filter paper has ovum grain every day; If any, the filter paper more renewed.
Hatching to larva detailed process is: collect the filter paper producing and have ovum grain, puts into the clean square plastic magazine that bottom is covered with moisturizing filter paper, is placed in climatic cabinate, to its hatching; Temperature in described climatic cabinate is 27 DEG C, the photoperiod is 14L:10D and humidity is 60% ± 10%, and the larva after hatching continues to carry out continuously rearing according to above-mentioned steps circulation; For studying the worm source that biological and abiotic factor provides worm state development progress more consistent on bright red hair spot moth population existence and the impact of breeding.
Embodiment 4
The indoor feeding method of bright red hair spot moth, it comprises the raising step of larva, pupa, adult and ovum, and specifically bright red hair spot moth newly hatched larvae is raised in larva raising device, is foodstuff, until it is pupated with Ficus microcarpa; Male and female pupa is separated in square plastic magazine after differentiating, after sprouting wings, pairing is placed in adult mating device; The pairing of the adult of post-coitum is placed in Adult worms producting eggs device and treats that it is laid eggs; Collect the filter paper producing in Adult worms producting eggs device and have ovum grain, put into the clean square plastic magazine that bottom is covered with moisturizing filter paper, be placed in climatic cabinate, every day notes moisturizing, to its hatching; After larvae hatch, larva is carried out continuously rearing according to above-mentioned step cycle.
Newly hatched larvae to the detailed process of pupating is: using Ficus microcarpa as foodstuff, and be placed on the filter in larva raising device, filter can be used as the platform and isolation larva, foodstuff and excreta of putting foodstuff, avoid polypide and foodstuff to be subject to excremental pollution, and finally cause larva Infective microbial death.With sterile writing brush picking newly hatched larvae on foodstuff, be placed in climatic cabinate after building plastic box cover and raise; Temperature in described climatic cabinate is 30 DEG C, the photoperiod is 14L:10D and humidity is 60% ± 10%; Within every two days, change a foodstuff, pick up filter simultaneously, the excreta of cleaning plastic box bottom, pupates until larva cocoons at box inwall.
Pupate to sprout wings detailed process be: with sterile tweezers, cocoon is taken out from larva raising device, after strip off cocoon, distinguishes male and female pupa; Respectively male and female pupa is put into two square plastic magazines of having sterilized, place wet cotton balls moisturizing in box, and square plastic magazine is placed in climatic cabinate, until it is sprouted wings; Temperature in described climatic cabinate is 30 DEG C, the photoperiod is 14L:10D and humidity is 60% ± 10%.
Sprout wings to mating detailed process and be: the female male worm just sprouted wings is placed in adult mating device, lets alone pangamy, 2 dark after dates take out adults, and become to be opposite in Adult worms producting eggs device and lay eggs, and check in Adult worms producting eggs device whether filter paper has ovum grain every day; If any, the filter paper more renewed.
Hatching to larva detailed process is: collect the filter paper producing and have ovum grain, puts into the clean square plastic magazine that bottom is covered with moisturizing filter paper, is placed in climatic cabinate, to its hatching; Temperature in described climatic cabinate is 30 DEG C, the photoperiod is 14L:10D and humidity is 60% ± 10%, and the larva after hatching continues to carry out continuously rearing according to above-mentioned steps circulation; For studying the worm source that biological and abiotic factor provides worm state development progress more consistent on bright red hair spot moth population existence and the impact of breeding.
As shown in the figure: described larva raising device, comprise: the square box 1 that the mouth the be made of plastics wide end is narrow and lid 2, lid 2 is placed on square box 1, lid 2 has box cover hole 3, box cover hole 3 is provided with cap bore net 4, and in square box 1, middle and lower part is provided with filter 5.
Described filter 5 is made up of stainless steel, and its width of mesh is 10 orders.
Described cap bore net 4 is gauze, and its width of mesh is 10-15 order.
Described adult mating device, comprise gauze 6 and the framework 7 in Fang Tizhuan, gauze 6 is arranged on six of the framework 7 in Fang Tizhuan, wherein one side is can switch, is convenient to put into and take out adult, is hung with the dactylethrae 9 that weight content is the hydromel of 10% is housed in adult mating device, an aperture is bored in dactylethrae lid, strip absorbent cotton 8 is through this hole, and staying long outside dactylethrae lid is 0.5cm-1cm, and remaining strip absorbent cotton 8 is all filled in dactylethrae 9.
Described Adult worms producting eggs device, comprise culture dish 10, vermiculite 11 and disposal plastic cup 12, vermiculite 11 is placed in culture dish 10, vermiculite 11 is coated with filter paper, disposal plastic cup 12 back-off covers vermiculite 11, offer through hole 13 bottom described disposal plastic cup 12, through hole 13 is that the cotton balls 14 of the hydromel of 10% is filled in stifled by being moistened with weight content.
Above-mentioned explanation is not limitation of the present invention, and the present invention is also not limited to above-mentioned example, those skilled in the art, and in essential scope of the present invention, the change made, remodeling, interpolation or replacement, all should belong to protection scope of the present invention.