CN101785445B - Scleroderma guani breeding method - Google Patents
Scleroderma guani breeding method Download PDFInfo
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- CN101785445B CN101785445B CN2010101347656A CN201010134765A CN101785445B CN 101785445 B CN101785445 B CN 101785445B CN 2010101347656 A CN2010101347656 A CN 2010101347656A CN 201010134765 A CN201010134765 A CN 201010134765A CN 101785445 B CN101785445 B CN 101785445B
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- 238000009395 breeding Methods 0.000 title claims abstract description 26
- 241000467911 Sclerodermus guani Species 0.000 title abstract 14
- 241000382353 Pupa Species 0.000 claims abstract description 29
- 230000001488 breeding effect Effects 0.000 claims abstract description 22
- 230000032669 eclosion Effects 0.000 claims abstract description 6
- 241000256844 Apis mellifera Species 0.000 claims description 156
- 230000013011 mating Effects 0.000 claims description 14
- 235000013601 eggs Nutrition 0.000 claims description 9
- 235000013311 vegetables Nutrition 0.000 claims description 9
- 230000008144 egg development Effects 0.000 claims description 8
- 239000002689 soil Substances 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 235000012907 honey Nutrition 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 3
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 238000009400 out breeding Methods 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 2
- 238000005516 engineering process Methods 0.000 abstract description 3
- 230000002265 prevention Effects 0.000 abstract description 2
- 241000560543 Osmia Species 0.000 abstract 7
- 102000002322 Egg Proteins Human genes 0.000 description 5
- 108010000912 Egg Proteins Proteins 0.000 description 5
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 5
- 235000011613 Pinus brutia Nutrition 0.000 description 5
- 241000018646 Pinus brutia Species 0.000 description 5
- 210000004681 ovum Anatomy 0.000 description 5
- 241000244206 Nematoda Species 0.000 description 4
- 241000124033 Salix Species 0.000 description 4
- 241000607479 Yersinia pestis Species 0.000 description 4
- 239000004927 clay Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000007789 sealing Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241001481710 Cerambycidae Species 0.000 description 2
- 241000218641 Pinaceae Species 0.000 description 2
- 241000018650 Pinus massoniana Species 0.000 description 2
- 235000011610 Pinus tabuliformis Nutrition 0.000 description 2
- ILRRQNADMUWWFW-UHFFFAOYSA-K aluminium phosphate Chemical compound O1[Al]2OP1(=O)O2 ILRRQNADMUWWFW-UHFFFAOYSA-K 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 230000000384 rearing effect Effects 0.000 description 2
- 230000008961 swelling Effects 0.000 description 2
- 241001609695 Anoplophora glabripennis Species 0.000 description 1
- 241001042196 Batocera horsfieldi Species 0.000 description 1
- 241001300233 Dendroctonus valens Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241001442207 Monochamus alternatus Species 0.000 description 1
- 241001197774 Semanotus Species 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 230000017448 oviposition Effects 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
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- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
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Abstract
The invention relates to a biological prevention and control technology, in particular to a scleroderma guani breeding method which solves the problem that the scleroderma guani breeding in large quantities is limited due to limited alternative host source of the present scleroderma guani. The scleroderma guani breeding method includes the following steps: a. the scleroderma guani and an alternative host are purchased for storage, and the alternative host is an osmia panzer; b. the osmia panzer is bred into a mature larva or a pupa; c. the mature larva or the pupa of the osmia panzer is bred into the scleroderma guani, so as to lead the scleroderma guani to carry out eclosion to form the adult scleroderma guani or the pupa. In the invention, the alternative hosts namely the osmia panzer and a male bee of the scleroderma guani are obtained through a large number of tests; the osmia panzer and the male bee are utilized to breed the scleroderma guani, which solves the problem that the scleroderma guani breeding in large quantities is limited due to limited alternative host source of the present scleroderma guani; meanwhile, the breeding method of the osmia panzer is provided, which can realize batch breeding of the osmia panzer, thereby obtaining a great quantity of alternative hosts on the basis of saving the cost and breeding a great quantity of adult scleroderma guani.
Description
Technical field
The present invention relates to the biological control technology, relate in particular to the mating system of natural enemy, be specially the mating system of a kind of swollen leg honeybee.
Background technology
Trunk borer is the important worm kind of in the forestry pest, the trunk borer in China every year causes huge economy and ecological loss to forest ecological environment, trunk borer generally is to bore moth to endanger in the host, and life cycle is very irregular, general chemical prevention and control method poor effect.
National quarantine property forestry pest pine nematode has caused huge threat from importing China abroad into to pinaceae plant, and in a single day pine tree catches an illness will soon be withered.Pine nematode is propagated by Monochamus alternatus, and at present China's pine nematode epidemic situation has been diffused into more than ten provinces and cities such as Guangdong, Hunan, Zhejiang, and only the trees cut down because of pine nematode of Zhejiang Province are just up to 200,000,000 strains; Red turpentine beetle also is national quarantine property forestry pest, pinaceae plants such as serious harm Chinese pine, lethal is very strong, import China into from the U.S. last century after, nearly ten million strain of Chinese pine causes death; Willow is an important species of China, and national willow planting area reaches 100,000,000 mu, and is also more than other national willow planting area summation of the world, based on the trunk borer of anoplophora glabripennis willow caused grave danger, area takes place every year be about 9,000,000 mu; Trunk borer harm such as red-edge longicorn, semanotus bifasciatus, mountain longicorn, batocera horsfieldi are also very big in addition.
The control of trunk borer is a world-famous puzzle always, trunk borer is sought hidden life, most of the time all bores moth and endangers in pin main body in its whole life, and general chemical control effect is very limited, and a large amount of simultaneously chemical pesticides that use also can cause serious environmental to pollute.Utilize natural enemy control trunk borer to have free from environmental pollution, can be for a long time, the advantage of lasting, stable control disaster, the present natural enemy of going up normal use of producing has swollen leg honeybee, generally adopts roundheaded borer or pupa to be alternative host and breeds swollen leg honeybee in a large number, is used for the control of trunk borer; Yet because the source of roundheaded borer or pupa is limited, it is higher to obtain cost, has limited breeding in a large number and using of swollen leg honeybee.
Summary of the invention
The present invention provides the mating system of a kind of swollen leg honeybee that can obtain to substitute host, low production cost in a large number and can breed on a large scale for the alternative host who the solves existing swollen leg honeybee problem that the swollen leg honeybee of limited restriction breeds in a large number of originating.
The present invention adopts following technical scheme to realize: the mating system of swollen leg honeybee, and is realized by following steps: a, buy swell leg honeybee and alternative host and preserve, described alternative host is the wall honeybee; B, carry out breeding of wall honeybee, make it breed into mature larva or pupa; C, the mature larva that utilizes the wall honeybee or pupa are raised swollen leg honeybee, and making swollen leg bee eclosion is adult or pupa.Selection wall honeybee host as an alternative records through a large amount of experiments, at first swollen leg honeybee and wall honeybee are carried out the low temperature preservation, after then the wall honeybee being bred into mature larva or pupa, the leg bee eclosion that then will swell is adult or pupa, and the wall honeybee breeds into mature larva or pupa is that those skilled in the art can draw by test of many times.
Breeding by following steps of wall honeybee realizes: a, buy nest pipe and hollow nest pipe that the wall bee imago is housed and preserve; Honeybee is warmed up in b, the wall honeybee cocoon taking-up that will be equipped with before booth vegetable is bloomed in the wall bee imago nest pipe; C, wall bee imago cocoon-break fly out to gather to enter in the hollow nest pipe and to lay eggs behind the pollen that booth vegetable blooms and seal; D, when becoming mature larva or pupa, preserves the egg development for the treatment of the wall honeybee.Growth characteristics according to the wall honeybee are bred the wall honeybee in booth, provide condition for the wall honeybee breeds needed temperature, light and feed, and it is better to breed effect.
Breeding as follows of wall honeybee realizes: a, buy nest pipe that the wall bee imago is housed and hollow nest pipe and the wall honeybee cocoon that will be equipped with in the wall bee imago nest pipe takes out; B, wall honeybee cocoon and hollow nest pipe are put into numerous beehive, be placed with feed in the casing of numerous beehive and be covered with the moistening soil of sterilizing in tank floor; C, wall bee imago cocoon-break fly out to take in to enter in the hollow nest pipe and to lay eggs behind the feed and seal; D, when becoming mature larva or pupa, preserves the egg development for the treatment of the wall honeybee, the wall honeybee is bred the restriction that is not subjected to the plant florescence by numerous beehive, be not subjected to the restriction of weather condition, intensification degree height, the speed of breeding is fast, can produce in enormous quantities at any time according to extermination of disease and insect pest demand, make breeding of swollen leg honeybee realize batch production, mass production.
Described feed be by parts by weight be 10 parts of honey, 10 portions of sugar and 20 parts of water fully mix the back add 3-6 part pollen stir evenly make or be that 20 parts of honey are put into 5 parts of water and stirred by parts by weight, and then add 2-3 part bean powder and 1-2 part pollen and fully stir to dry and make, record the quality of breeding that above-mentioned feed helps to improve the wall honeybee by test of many times.
Described alternative host is the honeybee drone, because the average weight of drone much larger than the average weight of worker bee, is about the twice of worker bee body weight, swollen leg honeybee bee hatching rate height, the bee yield of therefore breeding generation significantly increase.
The present invention draws alternative host's wall honeybee and the honeybee drone of swollen leg honeybee by a large amount of tests, thereby breed alternative host that swollen leg honeybee the overcome existing swollen leg honeybee problem that the swollen leg honeybee of limited restriction breeds in a large number of originating by wall honeybee and honeybee drone, the mating system that has proposed the wall honeybee simultaneously can realize that the mass of wall honeybee breeds, thereby can obtain to substitute host and a large amount of adults that breeds out swollen leg honeybee on cost-effective basis in a large number.
Embodiment
The mating system of swollen leg honeybee, is realized by following steps: a, buy swell leg honeybee and alternative host and preserve, described alternative host is the wall honeybee; B, carry out breeding of wall honeybee, make it breed into mature larva or pupa; C, the mature larva that utilizes the wall honeybee or pupa are raised swollen leg honeybee, and making swollen leg bee eclosion is adult or pupa.
Buying swollen leg honeybee and alternative host preserves, described alternative host is the wall honeybee, be specially: buy to select for use when swelling the leg honeybee and grow the apterergate healthy and strong, that fertility is strong, preserve stored refrigerated under the temperature that should place 2-5 ℃ when swelling leg honeybee kind honeybee and wall honeybee as kind of a honeybee.
Utilize mature larva or the pupa of wall honeybee to raise swollen leg honeybee, making swollen leg bee eclosion is adult or pupa, specifically comprise: wall honeybee mature larva or pupa are got the 1-3 head put into the shell of breeding that housing has the hole, then a plurality of shells of breeding are put into the box of breeding that has end cap, the leg honeybee kind that then will swell honeybee is put into and breeds box, swollen leg honeybee kind honeybee and wall honeybee are in the ratio inoculation of 1.2-2.0, the swollen leg honeybee kind honeybee in inoculation back can seek and substitute host and parasitic, to breed box then puts into incubator and cultivates, or put on the indoor cultivation frame and cultivate, be swollen leg bee imago or pupa until breeding, will be when the indoor cultivation frame is cultivated to the indoor shading treatment of carrying out, to improve the egg laying amount of swollen leg honeybee, numerous honeybee temperature is controlled at 23-30 ℃, and humidity is controlled at 50%-80%.A wall honeybee can breed out 30-60 chieftain honeybee.
Breeding by following steps of wall honeybee realizes: a, buy nest pipe and hollow nest pipe that the wall bee imago is housed and preserve; Honeybee is warmed up in b, the wall honeybee cocoon taking-up that will be equipped with before booth vegetable is bloomed in the wall bee imago nest pipe; C, wall bee imago cocoon-break fly out to gather to enter in the hollow nest pipe and to lay eggs behind the pollen that booth vegetable blooms and seal; D, when becoming mature larva or pupa, preserves the egg development for the treatment of the wall honeybee.
Buy nest pipe and hollow nest pipe that the wall bee imago is housed and preserve, be specially: the nest length of tube that the wall bee imago is housed is 180-200mm, and 7-10 nest chamber arranged in the nest pipe, and each nest is indoor a wall bee imago; The length of hollow nest pipe is 180-200mm, can build 7-10 nest chamber in each hollow nest pipe.
Before booth vegetable is bloomed, honeybee is warmed up in the taking-up of the wall honeybee cocoon in the nest pipe, specifically comprise: booth vegetable was bloomed preceding 2-3 days, the wall honeybee cocoon that will take out in the nest pipe was put under 12-16 ℃ the temperature warm honeybee 1-2 days, and humidity is controlled at 60%-80%, replenishes illumination with electricity-saving lamp again.
Wall bee imago cocoon-break flies out to gather to enter in the hollow nest pipe and to lay eggs behind the pollen that booth vegetable blooms and seals, specifically comprise: in the nest pipe support, put into hollow nest pipe, the nest pipe support is put into plastic greenhouse, nest pipe support opening one end is placed towards south, the soil water that water will about 30cm * 30cm before the nest pipe support waters, and is convenient to the wall honeybee and gets mudding nest chamber; Wall honeybee cocoon is placed in the tray and with plank covers, prevent sun direct projection, the sidewall of tray leaves opening, is convenient to wall bee imago cocoon-break and flies out, and wall honeybee cocoon is 1 with the quantity ratio of nest pipe: 1-2, after wall honeybee cocoon-break flies out, gather pollen and be stored in the nest pipe on the nest pipe support, and give birth to an ovum, seal with earth then, then produce second ovum and seal up, completely seal until the nest pipe is produced with earth.
When becoming mature larva or pupa, preserves the egg development for the treatment of the wall honeybee, specifically comprise: the hollow nest pipe that will produce full ovum is placed on indoor the preservation, indoor temperature is controlled at 20-30 ℃, humidity is controlled at 30%-70%, according to different wall honeybee kinds and different indoor temperatures, extract every day and check wall honeybee developmental state, when the wall honeybee develop into mature larva or cocoon pupate after, take out and under the moisture condition of 1-5 ℃ temperature, 50%-80%, preserve, allow the wall honeybee of other undeveloped mature continue to grow, after whole growths are finished, preserve under these conditions; After also can waiting the egg development of giving birth at last in the hollow nest pipe to become mature larva, preserve mature larva or the pupa taking-up of the wall honeybee in the hollow nest pipe stand-by under these conditions.
Breeding as follows of wall honeybee realizes: a, buy nest pipe that the wall bee imago is housed and hollow nest pipe and the wall honeybee cocoon that will be equipped with in the wall bee imago nest pipe takes out; B, wall honeybee cocoon and hollow nest pipe are put into numerous beehive, be placed with feed in the casing of numerous beehive and be covered with the moistening soil of sterilizing in tank floor; C, wall bee imago cocoon-break enter in the hollow nest pipe and the sealing clay of laying eggs after flying out and taking in feed, and then give birth to ovum sealing clay again, live the nest mouth of pipe with mudding then until hollow nest pipe is all produced to expire; D, when becoming mature larva or pupa, preserves the egg development for the treatment of the wall honeybee.Described feed is to be that 10 parts of honey, 10 portions of sugar and 20 parts of water fully mix back (or 5, or 6) part pollen that adds 3 and stir evenly and make by parts by weight; Described feed is to be that 20 parts of honey are put into 5 parts of water and stirred by parts by weight, and then adds 2 (or 3) part bean powderes and 1 (or 2) part pollen and fully stir to dry and make, and the unit of parts by weight is gram, two, jin or kilogram.
Wall honeybee cocoon and hollow nest pipe are put into numerous beehive, be placed with feed in the casing of numerous beehive and be covered with the moistening soil of sterilizing in tank floor, specifically comprise: hollow nest pipe is placed on the nest pipe support, the nest pipe support is put into numerous beehive again, thickness of soil is 8-12cm, and humidity is 80%-100%, and soil adopts the airtight fumigating system sterilization of aluminum phosphate, aluminum phosphate dosage by every cubic metre of 10-20g was fumigated 3-7 days under 20-30 ℃ temperature, took out the soil that abundant airing promptly is sterilized then.
Wall bee imago cocoon-break enters in the hollow nest pipe and the sealing clay of laying eggs after flying out and taking in feed, and then give birth to ovum sealing clay again, live the nest mouth of pipe with mudding then until hollow nest pipe is all produced to expire, specifically comprise: temperature was controlled at 22-30 ℃ when the wall honeybee laid eggs, and humidity is controlled at 30%-70%.
Described alternative host is the honeybee drone, the mating system of honeybee drone adopts existing method to breed, its main method is to put into the drone nest base frame of some in common numerous beehive, can obtain a large amount of honeybee drones, the ratio of worker bee nest base frame and drone nest base frame is 10 in the general numerous beehive: 5-8.
Below the instrument of breeding used in the above steps is elaborated:
Numerous beehive: the long 100cm of numerous beehive, wide 80cm, high 70cm is made by wooden frame, rear wall and lower wall are made by plank, and all the other each walls cover with gauze, prevent that the wall honeybee from running out of, the casing top is equipped with electricity-saving lamp, is used for replenishing light source daytime, is placed with the feed dish in numerous beehive.
Breed box: breeding box is flat cuboid zoarium, breeds on the end cap of box and has the hole, is fixed with gauze on the hole, is used to observe the situation of breeding of swollen leg honeybee and prevents that swollen leg honeybee from escaping.
Breed shell: breed that shell is made up of a housing and shell cap sleeve together.
Rearing tray: rearing tray is a square structure, and long is 10cm, and wide is 8cm, and height is 1cm, is placed with long 6cm in the dish, the wood chip of wide 1cm.
The nest pipe support: the nest pipe support is provided with 2 nest pipe lattice, the height of each nest pipe lattice is 12cm, wide is 10cm, dark is 25cm, nest pipe lattice bottom surface is far from ground 30cm, the baffle plate of 20-30cm is extended in the front side of nest pipe support, the nest pipe can be put into nest pipe lattice like this, simultaneously because the direct projection of the sun has been avoided in the effect of baffle plate.
Claims (6)
1. the mating system of a swollen leg honeybee is characterized in that: realized by following steps: a, buy swollen leg honeybee and alternative host preserves, described alternative host is the wall honeybee; B, carry out breeding of wall honeybee, make it breed into mature larva or pupa; C, the mature larva that utilizes the wall honeybee or pupa are raised swollen leg honeybee, and making swollen leg bee eclosion is adult or pupa.
2. the mating system of swollen leg honeybee according to claim 1 is characterized in that: breeding by following steps of wall honeybee realizes: a, buy nest pipe and hollow nest pipe that the wall bee imago is housed and preserve; Honeybee is warmed up in b, the wall honeybee cocoon taking-up that will be equipped with before booth vegetable is bloomed in the wall bee imago nest pipe; C, wall bee imago cocoon-break fly out to gather to enter in the hollow nest pipe and to lay eggs behind the pollen that booth vegetable blooms and seal; D, when becoming mature larva or pupa, preserves the egg development for the treatment of the wall honeybee.
3. the mating system of swollen leg honeybee according to claim 1 is characterized in that: breeding as follows of wall honeybee realizes: a, buy nest pipe that the wall bee imago is housed and hollow nest pipe and the wall honeybee cocoon that will be equipped with in the wall bee imago nest pipe takes out; B, wall honeybee cocoon and hollow nest pipe are put into numerous beehive, be placed with feed in the casing of numerous beehive and be covered with the moistening soil of sterilizing in tank floor; C, wall bee imago cocoon-break fly out to take in to enter in the hollow nest pipe and to lay eggs behind the feed and seal; D, when becoming mature larva or pupa, preserves the egg development for the treatment of the wall honeybee.
4. the mating system of swollen leg honeybee according to claim 3 is characterized in that: described feed is that 10 parts of honey, 10 portions of sugar and 20 parts of water fully mix the back and add 3-6 part pollen and stir evenly and make by parts by weight.
5. the mating system of swollen leg honeybee according to claim 3 is characterized in that: described feed is to be that 20 parts of honey are put into 5 parts of water and stirred by parts by weight, and then adds 2-3 part bean powder and 1-2 part pollen and fully stir to dry and make.
6. the mating system of swollen leg honeybee according to claim 3 is characterized in that: the long 100cm of numerous beehive, wide 80cm, high 70cm is made by wooden frame, and numerous beehive rear wall and lower wall are made by plank, all the other each walls are covered with gauze, and the casing top is provided with and is provided with the feed dish in electricity-saving lamp and the casing.
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| CN2010101347656A CN101785445B (en) | 2010-03-26 | 2010-03-26 | Scleroderma guani breeding method |
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| CN2010101347656A CN101785445B (en) | 2010-03-26 | 2010-03-26 | Scleroderma guani breeding method |
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| CN101785445A CN101785445A (en) | 2010-07-28 |
| CN101785445B true CN101785445B (en) | 2011-10-26 |
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| CN102524184B (en) * | 2011-12-22 | 2013-08-07 | 太原美林农业科技有限公司 | Preparation method of composite natural enemy spray |
| CN102885008A (en) * | 2012-10-31 | 2013-01-23 | 河北大学 | Artificial propagation method of Coccygomimus disparis |
| CN103004704A (en) * | 2013-01-05 | 2013-04-03 | 北京市西山试验林场 | Method for breeding Scleroderma guani Xiao et Wu by using barley pests as intermediate hosts |
| CN104996362B (en) * | 2015-06-25 | 2018-01-26 | 中国林业科学研究院森林生态环境与保护研究所 | A method of artificially breeding Scleroderma aureus and its application in preventing and controlling dry-boring pests |
| CN104920308B (en) * | 2015-06-25 | 2017-08-25 | 中国林业科学研究院森林生态环境与保护研究所 | A kind of swollen leg honeybee of the lucky fourth of artificial breeding larch and the method applied to preventing and treating mountain longicorn |
| CN104996236A (en) * | 2015-07-24 | 2015-10-28 | 贵州师范大学 | Method for preventing and treating dendroctonus valens LeConte by using scleroderma guani Xiao et Wus |
| CN105794727B (en) * | 2016-03-24 | 2018-06-01 | 镇远县醉美果业有限公司 | A kind of wall bee goes out cocoon method |
| CN106417190A (en) * | 2016-09-28 | 2017-02-22 | 天津拓华生物科技有限责任公司 | Method for pretreating sleroderma-guani alternate hosts |
| CN109043242A (en) * | 2018-07-24 | 2018-12-21 | 山东省农业科学院植物保护研究所 | A kind of man-made feeds and preparation method of Osmia. excavataAlfken |
| CN109874750B (en) * | 2019-03-22 | 2021-09-03 | 遵义市林业科学研究所 | Artificial breeding method of Cereus |
| CN110663443B (en) * | 2019-11-25 | 2021-04-06 | 澧县民丰林业科技有限公司 | Cultivation method for increasing yield and improving quality of oil tea |
| CN113661963A (en) * | 2021-07-27 | 2021-11-19 | 济南市林场 | Method for breeding tubificidae swollen legs by taking pupae prepearation of Ceratodon cupreum as host and application |
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