CN110036984A - A kind of method of low-coat scale raising bat moth - Google Patents

Abstract

The present invention relates to a low-cost method for raising bat moths on a large scale. A method for low-cost mass breeding of bat moths, comprising the following steps: collecting wild bat moth adults, mating them and laying fertilized eggs; preparing a mixed substrate, placing feed and fertilized eggs in the mixed substrate in a transparent container, and hatching larvae in Feeding and development in the substrate; when 80%-100% of the larvae develop to the 4th instar, they are transferred to the transparent container where the feed and mixed substrate are put in by punching and feeding; when 80%-100% of the larvae develop to the 6th instar, the The method of punching and throwing is transferred to a transparent container for throwing mixed substrate and feed at low temperature until it pupates; the pupae emerge to obtain bat moth adults. The invention adopts wild bat moth adults as the insect source and mixed substrate as the feeding substrate, and achieves large-scale feeding through the method of indoor circulation cultivation, which not only avoids the damage to the grassland ecological environment caused by the large-scale excavation of bat moth pupae and plateau humus, but also The labor and transportation costs of raw material collection are reduced.

Description

A low-cost method for mass rearing bat moths

technical field

The invention relates to a method for raising bat moths, in particular to a method for raising bat moths on a low-cost scale.

Background technique

Cordyceps sinensis is a special and rare traditional Chinese medicine in my country, mainly distributed in alpine meadow areas such as Tibet, Qinghai, Sichuan, Yunnan and Gansu. The formation of Cordyceps sinensis is closely related to altitude, vegetation, temperature and humidity, light, air pressure and rain. The growth conditions are very harsh, and due to the degradation of the plateau ecological environment and artificial over-excavation, the production of Cordyceps sinensis has dropped significantly. Cultivation has become an important trend in the development of the Cordyceps sinensis industry.

Large-scale breeding of insect sources is the primary key point for artificial cultivation of Cordyceps sinensis and improving the yield of Cordyceps sinensis. As the obligate host of Cordyceps sinensis, bat moth insects are raised indoors through generational reproduction to solve the problem of insect sources, and at the same time reduce the cost of feeding, and become a large-scale breeding of bats. Critical steps in moth insects.

Under natural conditions, bat moth larvae live in the soil of alpine meadows. In order to simulate its wild environment during the breeding process, a large amount of alpine meadow soil in the production area is often excavated as a feeding substrate for bat moth larvae. At the same time, the process of collecting insect sources Excavation of wild bat moth pupae is common in China, which increases labor and transportation costs on the one hand, and causes huge damage to the ecological environment of Cordyceps sinensis producing areas on the other hand. The feed used in the larval rearing process is mostly natural roots or tubers such as Polygonum bulbar, fern hemp or carrot, which are directly fed to the larvae. Stay high.

SUMMARY OF THE INVENTION

(1) Technical problems to be solved

In order to solve the problems existing in the prior art, the present invention provides a method for reducing the feeding amount of feed and the cost of large-scale breeding of bat moths by adopting different culture conditions at different growth stages.

(2) Technical solutions

In order to achieve the above-mentioned purpose, the main technical scheme adopted in the present invention includes:

The present invention provides a low-cost method for large-scale breeding of bat moths, which comprises the following steps:

S1 collection of fertilized eggs: collect wild bat moth adults, and make them mate to lay fertilized eggs;

S2 Incubation of fertilized eggs: prepare a mixed matrix, put feed and fertilized eggs in the mixed matrix and hatch to obtain larvae, which are raised in the mixed matrix until 80% to 100% of the larvae develop to the 4th instar;

S3 transfer feeding of larvae: transfer the 4th instar larvae obtained in step S2 to a transparent container where feed and mixed substrate are put in for rearing, until 80% to 100% of the larvae develop to 6th instar or above;

The pupation of S4 larvae: the larvae more than 6 instars obtained in step S3 are transferred to the transparent container that throws mixed substrate and feed and raise, until it pupates;

Emergence of S5 pupa: The pupa emerges to obtain the adult bat moth.

Among them, the feed referred to in this scheme refers to the tissue removed from the living animals and plants, and its water content, protein, fat, etc. have not undergone chemical changes. Among them, wild bat moth adults are preferably collected from alpine meadows, and adults are selected as the source of insects to avoid the damage to the grassland ecological environment caused by artificially excavating a large number of pupae. Abandon the continuous provision of large amounts of feed to the larvae, and instead reduce the food supply to the larvae at the age of 6 years and above and implement low-temperature cultivation to promote the pupation of the old larvae, and avoid the larvae that have been feeding a lot of feed, appearing multiple peeling and leggy And so on, but also save the feed. The breeding method of this scheme adopts indoor self-propagation, which avoids excessive demand for natural sources of bat moths and damage to the ecological environment of the production area of Cordyceps sinensis. Subsequent low-temperature cultivation to pupation and eclosion reduces the developmental speed and increases or maintains the fecundity of the eclosion adults.

In a preferred solution of the method for rearing bat moths, it further includes step S6: the bat moth adults that have emerged in step S5 are interbred to lay fertilized eggs, and the steps after step S1 are continued to cultivate cyclically. Among them, continuous cycle cultivation to achieve large-scale cultivation.

In a preferred solution of the method for raising bat moths, the mixed substrate includes the following raw materials: understory soil and coconut bran.

Among them, the understory soil is the loose soil under the understory humus layer 40cm. In this scheme, the commonly used substrates such as humus soil and peat soil, which are similar to the native environment of bat moth larvae, are discarded, and the mixed substrate is prepared by using understory soil and coconut bran, which has a low organic matter content and is not prone to mold infection; , the organic matter content in the loose forest soil is low, the particles are loose and relatively difficult to compact, and the air permeability and drainage are good; the coconut bran is relatively fluffy, with more fibrous substances and good water retention. It has the advantages of moisture retention, water retention, etc., which can jointly play the role of moisturizing, ventilation and waterlogging prevention.

In the preferred scheme of the method for raising bat moths, the ratio of understory soil to coconut bran is 8:1 to 3 by weight.

Among them, the selected understory soil and coconut bran are sterilized by sun exposure, high temperature sterilization, microwave sterilization or ultraviolet lamp sterilization, etc., and then stirred to form a mixed matrix, the humidity is controlled at 45-60%, and the pH is adjusted to Between 5.5 and 6.5.

In a preferred solution of the method for raising bat moths, in step S2, the feed includes the following components: fern root and ranba seeds; after the fern root and Ranba seeds are mixed, they are placed in the middle and upper layers of the mixed substrate 8～8 At a depth of 15 cm, the weight ratio of the fern hemp tuber root and the burnt seed is 3-5:1.

In a preferred scheme of the method for raising bat moths, the mixed matrix in step S2 is cultured for 15-45 days after feeding the feed, and then used for hatching fertilized eggs.

The temperature of the environment for hatching the fertilized eggs is 12-18° C. and the relative humidity is 60-80%. In step S2, 0.5-3.0 g of feed is put into the mixed matrix for every 4 fertilized eggs;

This scheme abandons the simple use of plant roots or tubers as larvae feed in the prior art, and uses a small amount of feed plant seedlings to cultivate a huge root system to provide rich feed for the larvae. food to improve survival.

In a preferred scheme of the method for rearing bat moths, the fertilized eggs are placed at a depth of 3-8 cm in the mixed substrate.

In a preferred solution of the method for raising bat moths, in step S2, the fertilized eggs are placed on the feed in the mixed matrix, and the surface of the mixed matrix is covered with a moist medium to keep moisture.

Among them, the method of throwing the fertilized eggs is to take the eggs of the bat moth as the insect source, and make an egg card. About 7 days before the eggs hatch, they are put into a nylon mesh of 30-60 meshes and sterilized in alcohol for 20 s or in a sodium hypochlorite solution for 2-3 days. After 10 minutes, put it in the middle and upper layers of the mixed matrix at a depth of 3-8 cm, and put the Ranba seeds at 0.10-0.25g per egg at the egg placement position. The egg card is buckled above the Ranba seeds. Providing sufficient feed for the newly hatched larvae is beneficial to improve the survival rate; using a moist medium to moisturise the surface of the substrate is conducive to the hatching of eggs, improving the hatching rate, and at the same time blocking external sources of infection.

In a preferred scheme of the method for raising bat moths, in step S3, the humidity of the mixed substrate is controlled to be 45-60%, and after the larvae and the feed are put in order, they are placed in an environment with a temperature of 10-15°C and a relative humidity of 50-70% feed under. In step S3, 0.5-3.0 g of feed is put into the mixed matrix according to each larva.

Among them, when the larvae developed to the 4th instar, the method of punching and feeding was adopted, and the feed was placed on the surface layer of the mixed substrate. Punching and casting can effectively reduce the chance of larvae killing each other, and the larvae can also burrow into the substrate faster, reducing the chance of being air-dried or exposed to external sources of infection. The feed is placed on the surface of the substrate, which is convenient for replacement and observation, and avoids the situation that the larvae are infected and died in batches due to the rot of the feed in the substrate.

In a preferred scheme of the method for raising bat moths, in step S4, 0.5-1.0 g of feed is put into the mixed matrix per larva, and the aged larvae are cultivated at low temperature, and the cultivation temperature is 2-13 °C.

In this scheme, when the larvae develop to the age of 6 or more, they are transferred to a new container with mixed substrate and feed to make them pupate. The substrate temperature of the container simulates the ground temperature of the natural environment, and the pre-pupa pupation process of the old larvae is cultivated at low temperature to reduce the development speed. The temperature change range is controlled at 2 to 13 °C, and the culture temperature is gradually decreased monthly and then increased. The monthly temperature change range is 8 °C to 11 °C. The specific monthly temperature adjustment method is but not limited to 13 °C → 9 °C →5°C→9°C→13°C, or 13°C→7°C→2°C→7°C→13°C, cycle until old larvae pupate, and the incubation time is 3 to 5 months.

In the preferred scheme of the method for raising bat moths, the mixed substrates are all packed in transparent containers with a capacity of more than 30L, and several 1-3cm holes are punched on the upper edge of the container to enhance ventilation and reduce the probability of mildew on the substrate. At the same time, the transparent container facilitates visual observation of the humidity, pollution and larval activity in the substrate.

In the preferred solution of the method for rearing bat moths, Ranba seeds are seeds with pearly buds for planting Polygonum globosa, and with pearl buds, they can promote shoots and form root systems more quickly.

(3) Beneficial effects

The beneficial effects of the present invention are:

1. The present invention adopts wild bat moth adults as insect source and preparation mixed matrix as feeding matrix, which not only avoids the damage caused by artificial large-scale excavation of wild bat moth pupae and plateau humus to the ecological environment of Cordyceps sinensis producing areas, but also reduces the amount of harvesting. High cost of digging manpower and transportation etc.

2. The present invention forms a huge root system by placing feed plant seedlings in the mixed substrate, which is beneficial to the feeding of young larvae, saves a lot of feed, and avoids the uneconomical phenomenon caused by a large amount of feed.

3. In the present invention, when the larvae develop to the 4th instar, they are transferred to the transparent container for feeding the substrate and the feed, and the method of punching is adopted to effectively reduce the probability that the larvae kill each other. And the probability of contact with external infection sources, and the feed is placed on the surface of the substrate, which is convenient for replacement and observation, and avoids the rotting of the feed in the substrate and the death of larvae caused by batch infection. The aged larvae are transferred to the transparent containers where the substrate and feed are put in for rearing; they are cultivated at low temperature until they pupate, which reduces the developmental speed and improves or maintains the fecundity of the adults.

Detailed ways

In order to make the objectives, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be described in detail below. Obviously, the described embodiments are only some, but not all, embodiments of the present invention. Based on the embodiments of the present invention, all other implementations obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present invention.

Example 1

A method for low-cost mass breeding of bat moths, comprising the following steps:

Collection of S1 fertilized eggs: A small amount of live wild bat moth adults in the alpine meadow area were collected in the Cordyceps sinensis production area, and the adults were mated in the sarong at dusk in the origin area, and the fertilized eggs were collected and brought back to the laboratory. , Preservation under environmental conditions with relative humidity of 60-75%;

S2 mixed matrix preparation: indoor use of understory soil and coconut bran is formulated into a mixed matrix with a dry weight ratio of 8:2, and the humidity is controlled at 45%. After stirring evenly, put it into a 30L transparent container for use;

S3 Feeding and Cultivation: The feed is placed in the proportion of 1 g feed per 4 fertilized eggs, and the feed includes fern and hemp tubers and Ranba seeds with a weight ratio of 4:1. Cultivated for 30 days at 16°C and under the conditions of sufficient water and light, so that the tuberous roots of fern and the seeds of Ranba germinate and form a huge root system;

S4 Egg retrieval and hatching: take bat moth eggs and make egg cards. About 7 days before hatching, put them into 30-60 mesh nylon nets, sterilize them in alcohol for 20s or sodium hypochlorite solution for 2 minutes, and place them at a depth of 3cm in the mixed matrix , and add 0.2g of Ranba seeds at the placement position, the egg card is buckled upside down above the Ranba seeds, the surface of the substrate is covered with a moist medium to keep moisture, and it is placed in an indoor temperature of 18 ℃ and a relative humidity of 65%. The environment is incubated under the environment;

Transfer and rearing of S5 larvae: Regular sampling, it is found that 80% to 100% of the larvae develop to the 4th instar, transfer to a transparent container with feed and mixed matrix to continue feeding, and the humidity of the mixed matrix is kept at 50%; method, put 1 larva in each hole, and put the feed on the surface of the matrix. The feed includes common species such as carrots, and put it in the amount of 1 g of feed per larva, and place it in an environment with a temperature of 13°C and a relative humidity of 50%.

Pupation of S6 larvae: When it is found that 80% to 100% of the larvae have developed into 6 instar larvae and above, they are transferred to a transparent container where feed and substrate are put in by punching and feeding. The temperature of the culture room simulates the ground temperature of the natural environment , The feed includes common species such as carrots, and can also be fern and hemp tubers and burnt seeds. When the culture temperature drops to 13°C, the culture temperature is gradually lowered monthly and then raised, with a temperature change of 8°C. The specific monthly temperature is adjusted to 13°C→9°C→5°C→9°C→13°C until old age. Larvae pupate and culture for 3 to 5 months.

S7 Emergence: as the temperature gradually increases, the pupa emerges to obtain adult bat moths, and the male and female adults are manually and accurately captured and placed in a sarong for mating to obtain fertilized eggs, and step S1 is repeated for cyclic cultivation.

Example 2

A method for low-cost mass breeding of bat moths, which comprises the following steps:

Collection of S1 fertilized eggs: A small amount of live wild bat moth adults in the alpine meadow area were collected in the Cordyceps sinensis production area, and the adults were mated in the sarong at dusk in the origin area, and the fertilized eggs were collected and brought back to the laboratory. , Preservation under environmental conditions with relative humidity of 60-75%;

S2 mixed matrix preparation: indoor use of understory soil and coconut bran are prepared into a mixed matrix according to the dry weight ratio of 8:1, the humidity is controlled at 45%, and after stirring evenly, it is put into a 30L transparent container for use;

S3 Feeding and Cultivation: The feed is placed in the proportion of 0.5g feed per 4 fertilized eggs, and the feed includes fern and hemp roots and Ranba seeds with a weight ratio of 3:1, placed in the upper layer of the mixed matrix 12cm deep, at 16 Cultivated for 45 days under the conditions of ℃, sufficient water and light, so that the tuberous root of fern and ramie seeds germinate and form a huge root system;

S4 egg retrieval and hatching: take bat moth eggs and make egg cards. About 7d before hatching, put them into 30-60 mesh nylon nets, sterilize them in alcohol for 20s or sodium hypochlorite solution for 2 minutes, and place them at 8cm of the mixed matrix. Add 0.10g of Ranba seeds per egg at the placement position, the egg card is buckled upside down above the Ranba seeds, the surface of the substrate is covered with a moist medium to keep moisture, and incubated in an environment with an indoor temperature of 12°C and a relative humidity of 60%;

Transfer and feeding of S5 larvae: Regular sampling, when 80% to 100% of the larvae are found to have developed to the 4th instar, they are transferred to a transparent container where feed and mixed substrate are placed, and the humidity of the mixed substrate is kept at 45%; The method is to put 1 larva in each hole, and put the feed at a depth of 3cm on the surface of the substrate. The feed includes common species such as carrots, and can also be fern root or Ranba seeds; put 0.5g of feed per larva. Raised in an environment with a temperature of 10°C and a relative humidity of 50%;

Pupation of S6 larvae: When it is found that 80% to 100% of the larvae have grown into aged larvae over 6 years old, they are transferred to a transparent container with feed and mixed substrates by punching and feeding. The feed includes common species such as carrots. , can also be fern root tuber and burnt seeds. The temperature of the culture room simulates the ground temperature of the natural environment. The temperature of the culture room is gradually lowered and then raised monthly. The temperature change range is 9°C month by month. The specific monthly adjusted temperature is 13°C→9°C→4°C→9°C→13°C , until the old larvae pupate, the culture time is 3 to 5 months.

S7 Emergence: as the temperature gradually increases, the pupa emerges to obtain adult bat moths, and the male and female adults are manually and accurately captured and placed in a sarong for mating to obtain fertilized eggs, and step S1 is repeated for cyclic cultivation.

Example 3

A method for low-cost mass breeding of bat moths, comprising the following steps:

Collection of S1 fertilized eggs: A small amount of live wild bat moth adults in the alpine meadow area were collected in the Cordyceps sinensis production area, and the adults were mated in the sarong at dusk in the origin area, and the fertilized eggs were collected and brought back to the laboratory. , Preservation under environmental conditions with relative humidity of 60-75%;

S2 mixed matrix preparation: indoor use of understory soil and coconut bran is formulated into a mixed matrix with a dry weight ratio of 8:3, and the humidity is controlled at 60%. After stirring evenly, put it into a 40L transparent container for later use;

S3 Feeding and Cultivation: The larvae feed is placed in the proportion of 3 g of feed per 4 fertilized eggs, and the feed includes fern root and Ranba seeds with a weight ratio of 5:1, placed in the upper layer of the mixed matrix 10cm deep, at 20 Cultivated for 25 days under the conditions of sufficient moisture and light, so that the tuberous roots and Ranba seeds germinate and form a huge root system;

S4 egg retrieval and hatching: take bat moth eggs, make egg cards, put them into 30-60 mesh nylon nets about 7d before hatching, sterilize them in alcohol for 20s or sodium hypochlorite solution for 3 minutes, and place them at 6cm of the mixed matrix. And add Ranba seeds according to 0.25g per egg in the placement position, the egg card is buckled above the Ranba seeds, the surface of the substrate is covered with a moist medium to keep moisture, and it is placed in an indoor temperature of 18 ℃ and a relative humidity of 80%. The environment is incubated under the environment;

Transfer feeding of S5 larvae: Regular sampling, when 80% to 100% of the larvae are found to have developed to the 4th instar, they are transferred to a transparent container where feed and mixed substrate are placed, and the humidity of the mixed substrate is kept at 55%; method, put 1 larva in each hole, put the feed at a depth of 6 cm on the surface of the substrate, the feed includes common species such as carrots, and put 3 g of feed per fertilized egg, and place it at a temperature of 15 ° C and a relative humidity of 70% raised in an environment;

Pupation of S6 larvae: When it is found that 80% to 100% of the larvae have developed into aged larvae over 6 years old, they are transferred to transparent containers with feed and mixed substrates by punching and feeding. The feed includes common species such as carrots. It can also be fern root tuber and burnt seeds. The temperature of the culture room simulates the ground temperature of the natural environment. The temperature of the culture room is gradually decreased and then increased monthly. The temperature change range from month to month is 8°C. The specific temperature adjustment method is 13°C→9°C→5°C→9°C→13 ℃, until the old larvae pupate, and the culture time is 3 to 5 months.

S7 Emergence: as the temperature gradually increases, the pupa emerges to obtain adult bat moths, and the male and female adults are manually and accurately captured and placed in a sarong for mating to obtain fertilized eggs, and step S1 is repeated for cyclic cultivation.

Example 4

A method for low-cost mass breeding of bat moths, which comprises the following steps:

Collection of S1 fertilized eggs: A small amount of live wild bat moth adults in the alpine meadow area were collected in the Cordyceps sinensis production area, and the adults were mated in the sarong at dusk in the origin area, and the fertilized eggs were collected and brought back to the laboratory. , Preservation under environmental conditions with relative humidity of 60-75%;

S2 mixed matrix preparation: indoor use of understory soil and coconut bran is formulated into a mixed matrix with a dry weight ratio of 8:3, and the humidity is controlled at 50%. After stirring evenly, put it into a 30L transparent container for use;

S3 Feeding and Cultivation: The larvae feed is placed in the proportion of 3 g of feed per 4 fertilized eggs, and the feed includes fern and hemp roots and Ranba seeds with a weight ratio of 4:1, placed in the upper 15cm depth of the mixed matrix, at 17 Cultivated for 45 days under the conditions of ℃, sufficient water and light, so that the tuberous root of fern and ramie seeds germinate and form a huge root system;

S4 egg retrieval and hatching: take bat moth eggs and make egg cards. About 7d before hatching, put them into 30-60 mesh nylon nets, sterilize them in alcohol for 20s or sodium hypochlorite solution for 3 minutes, and place them at 5cm of the mixed matrix. The placement position needs to add 0.18g of each egg with Ranba seeds, the egg card is buckled above the Ranba seeds, the surface of the substrate is covered with a moist medium to keep moisture, and it is placed in an indoor temperature of 15 ℃ and a relative humidity of 70%. Incubate in an environment;

Transfer feeding of S5 larvae: Regular sampling, when 80% to 100% of the larvae are found to have developed to the 4th instar, they are transferred to a transparent container where feed and mixed substrate are placed, and the humidity of the mixed substrate is kept at 55%; method, put 1 larva in each hole, put the feed on the surface layer 2cm, the feed includes common species such as carrots, put 0.5g of feed per larva, and put it in an environment with a temperature of 15°C and a relative humidity of 60%. rearing;

Pupation of S6 larvae: When it is found that 80% to 100% of the larvae have developed into aged larvae over 6 years old, they are transferred to transparent containers with feed and mixed substrates by punching and feeding. The feed includes common species such as carrots. It can also be fern root tuber and burnt seeds. The temperature of the culture room simulates the ground temperature of the natural environment. The temperature of the culture room is gradually decreased and then increased monthly. The temperature change range from month to month is 10 °C. The specific monthly adjusted temperature is 13 °C → 8 °C → 3 °C → 8 °C → 13 °C , until the old larvae pupate, the culture time is 3 to 5 months.

S7 Emergence: as the temperature gradually increases, the pupa emerges to obtain adult bat moths, and the male and female adults are manually and accurately captured and placed in a sarong for mating to obtain fertilized eggs, and step S1 is repeated for cyclic cultivation.

Example 5

A method for low-cost mass breeding of bat moths, which comprises the following steps:

Collection of S1 fertilized eggs: A small amount of live wild bat moth adults in the alpine meadow area were collected in the Cordyceps sinensis production area, and the adults were mated in the sarong at dusk in the origin area, and the fertilized eggs were collected and brought back to the laboratory. , Preservation under environmental conditions with relative humidity of 60-75%;

S2 mixed matrix preparation: indoor use of understory soil and coconut bran are prepared into a mixed matrix according to the dry weight ratio of 8:1, the humidity is controlled at 60%, and after stirring evenly, it is placed in a 35L transparent container for use;

S3 Feeding and Cultivation: The larvae feed is placed at a ratio of 1 g feed per 4 fertilized eggs, and the feed includes fern and hemp roots and Ranba seeds with a weight ratio of 5:1, placed at a depth of 8 cm in the upper layer of the mixed matrix, at 19 Cultivated for 30 days under the conditions of sufficient water and light, so that the tuberous roots and Ranba seeds germinate and form a huge root system;

S4 egg retrieval and hatching: take bat moth eggs and make egg cards. About 7d before hatching, put them into 30-60 mesh nylon nets, sterilize them in alcohol for 20s or sodium hypochlorite solution for 3 minutes, and place them at 7cm of the mixed matrix. The placement position needs to add 0.25g of Ranba seeds per egg, the egg card is buckled above the Ranba seeds, the surface of the substrate is covered with a moist medium to keep moisture, and it is placed in an indoor temperature of 13 ℃ and a relative humidity of 80%. Incubate in an environment;

Transfer and feeding of S5 larvae: Regular sampling, when 80% to 100% of the larvae are found to have developed to the 4th instar, they are transferred to a transparent container where feed and mixed substrate are placed, and the humidity of the mixed substrate is kept at 46%; method, put 1 larva in each hole, put the feed on the surface layer 6cm, the feed includes carrots and other common species, and put 2g of feed per larva, and put it in an environment with a temperature of 11°C and a relative humidity of 68%. ;

Pupation of S6 larvae: When it is found that 80% to 100% of the larvae develop into aged larvae over 6 years old, they are transferred to a transparent container with feed and mixed substrates by punching and feeding. The feed includes common species such as carrots. , can also be fern root tuber and burning ba seeds. The temperature of the culture room simulates the ground temperature of the natural environment. The temperature of the culture room is gradually reduced and then increased monthly. The temperature change range from month to month is 8°C. The specific temperature adjustment method is 13°C→9°C→5°C→9°C→13 ℃, until the old larvae pupate, and the culture time is 3 to 5 months.

S7 Emergence: as the temperature gradually increases, the pupa emerges to obtain adult bat moths, and the male and female adults are manually and accurately captured and placed in a sarong for mating to obtain fertilized eggs, and step S1 is repeated for cyclic cultivation.

Example 6

A method for low-cost mass breeding of bat moths, which comprises the following steps:

Collection of S1 fertilized eggs: A small amount of live wild bat moth adults in the alpine meadow area were collected in the Cordyceps sinensis production area, and the adults were mated in the sarong at dusk in the origin area, and the fertilized eggs were collected and brought back to the laboratory. , Preserved under environmental conditions of relative humidity of 60-75%;

S2 mixed matrix preparation: indoor use of understory soil and coconut bran is formulated into a mixed matrix according to the dry weight ratio of 8:2, the humidity is controlled at 48%, and after stirring evenly, it is placed in a 40L transparent container for use;

S3 Feeding and Cultivation: The larvae feed is placed in an amount of 2g per 4 fertilized eggs, and the feed includes fern and hemp roots and Ranba seeds with a weight ratio of 4:1, and is placed in the upper layer of the mixed matrix 10cm deep, at 20 Cultivated for 25 days under the conditions of sufficient moisture and light, so that the tuberous roots and Ranba seeds germinate and form a huge root system;

S4 egg retrieval and hatching: take bat moth eggs and make egg cards. About 7d before hatching, put them into 30-60 mesh nylon nets, sterilize them in alcohol for 20s or sodium hypochlorite solution for 2 minutes, and place them at 8cm of the mixed matrix. The placement position needs to add 0.15g of Ranba seeds per egg, the egg card is buckled above the Ranba seeds, the surface of the substrate is covered with a moist medium to keep moisture, and it is placed in an indoor temperature of 17 ℃ and a relative humidity of 65%. The environment incubates;

Transfer and feeding of S5 larvae: Regular sampling, when 80% to 100% of the larvae are found to have developed to the 4th instar, they are transferred to a transparent container where feed and mixed substrate are placed, and the humidity of the mixed substrate is kept at 51%; method, put 1 larva in each hole, put the feed on the surface of the substrate 3cm, the feed includes common species such as carrots, and put 2.5g of feed per larva, and place it in an environment with a temperature of 13°C and a relative humidity of 55%. rearing;

Pupation of S6 larvae: When it is found that 80% to 100% of the larvae have developed into aged larvae of 6 instars and above, they are transferred to transparent containers with feed and mixed substrates by punching and feeding. Feeds include carrots and other common species, it can also be fern root tuber and burnt seed. The temperature of the culture room simulates the ground temperature of the natural environment. The temperature of the culture room is gradually reduced and then increased monthly. The temperature change range from month to month is 11°C. The specific temperature adjustment method is 13°C→8°C→2°C→8°C→13 ℃, until the old larvae pupate, and the culture time is 3 to 5 months.

S7 Emergence: as the temperature gradually increases, the pupa emerges to obtain adult bat moths, and the male and female adults are manually and accurately captured and placed in a sarong for mating to obtain fertilized eggs, and step S1 is repeated for cyclic cultivation.

The method for raising bat moths of the present invention was selected as the test group, and the larvae were raised without substrate in the laboratory as the comparative example, the glass petri dish was used as the raising container, and carrots were used as feed. See Table 1 and Table 2 for the cost of feeding human resources:

Table 1 Survival and reproduction of bat moths under different feeding conditions

Table 2 Estimated cost of raising 100,000 larvae

Conclusion: Using the low-cost mass rearing method of the present invention, the hatching rate of bat moth eggs, the survival rate of larvae, the pupation rate of aged larvae, and the pupa emergence rate are all higher than those of the comparative example, and the feeding cost is only 1/2 of the comparative example. The viability and fecundity of the bat moth grown in the environment described in the invention are higher than those of the comparative example, which is beneficial to the survival, pupation and emergence of the bat moth larvae, and is also beneficial to the reduction of the feeding cost.

The above are only specific embodiments of the present invention, but the protection scope of the present invention is not limited to this. Any person skilled in the art can easily think of changes or substitutions within the technical scope disclosed by the present invention. should be included within the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims (10)

1. a kind of method of low-coat scale raising bat moth, which is characterized in that itself the following steps are included:

The collection of S1 insemination ovum: wild bat moth adult is acquired, its mating is made to give birth to insemination ovum；

The hatching of S2 insemination ovum: preparing mixed-matrix, and feed and insemination ovum are launched in mixed-matrix and its hatching is made to obtain children Worm, larva feeding in mixed-matrix is developed, until 80%~100% larvae development to 4 ages；

The transfer of S3 larva is raised: 4 instar larvaes obtained by step S2 are transferred in the transparent vessel for launching feed and mixed-matrix Raising, until 80%~100% larvae development is to 6 more than age；

S4 larva pupates: the 6 ages above larva obtained by step S3 is transferred in the transparent vessel for launching mixed-matrix and feed Raising is cultivated until it is pupated；

The emergence of S5 pupa: pupa, which is sprouted wings, obtains bat moth adult.

2. the method for low-coat scale raising bat moth as described in claim 1, which is characterized in that it further includes step S6: The bat moth adult to sprout wings in step S5 is mated given birth to insemination ovum, and the step after continuing step S1 realizes that circulation is cultivated.

3. a kind of method of low-coat scale raising bat moth as described in claim 1, it is characterised in that: the mixed-matrix Including hayashishita soil and coco bran.

4. the method for low-coat scale raising bat moth as described in claim 1, which is characterized in that in step S2, the feeding Material includes following components: fern fiber crops root tuber and combustion bar seed；

Launched 8~15cm at the middle and upper levels of mixed-matrix at after the fern fiber crops root tuber and combustion bar seed mixing, the fern fiber crops root tuber with The weight ratio that combustion bar seed is launched is 3~5:1.

5. the method for low-coat scale raising bat moth as described in claim 1, it is characterised in that: mixed described in step S2 Matrix cultivates 15~45d after launching feed, is used further to hatching insemination ovum.

6. the method for low-coat scale raising bat moth as described in claim 1, it is characterised in that: ovum of inseminating in step S2 is thrown It is placed on the top of feed in mixed-matrix, surface covers wet medium moisturizing.

7. the method for low-coat scale raising bat moth as claimed in claim 6, it is characterised in that: ovum of inseminating in step S2 is thrown It is placed on the depths 3~8cm of mixed-matrix.

8. the method for low-coat scale raising bat moth as described in claim 1, which is characterized in that mixed-matrix in step S2 It is interior to launch 0.5~3.0g feed by every 4 insemination ovum；

Or 0.5~3.0g feed is launched by every larva in mixed-matrix in step S3；

Or 0.5~1.0g feed is launched by every mature larvae in mixed-matrix in step S4.

9. the method for low-coat scale raising bat moth as described in claim 1, it is characterised in that: in step S4, larva is adopted With low temperature breeding method, the temperature of cultivation is 2~13 DEG C.

10. the method for low-coat scale raising bat moth as claimed in claim 9, it is characterised in that: in step S4, low temperature training It raises an infant worm, cultivates temperature change using monthly gradually reducing again raised mode.