CN106719443B - Method for breeding brown sandflies by utilizing aphids - Google Patents

Abstract

The invention discloses a method for using aphids to breed brown lacewings, which optimizes the breeding conditions of brown lacewings and significantly improves the egg laying amount, egg hatching rate, larval predation and adult emergence rate of the brown lacewings. , which provided technical support for the large-scale reproduction of Lacewings.

Description

A kind of method of using aphids to breed brown flies

technical field

The invention relates to a method for breeding natural enemy insects, in particular to a method for breeding brown lacewings by using aphids, and belongs to the technical field of biological control of agricultural pests.

Background technique

The main insects used for biological control of Neuroptera are Lacewings. Lacewings are predatory insects with a wide distribution and a wide variety of about 1,300 species. Lacewings can prey on aphids, scale insects, red spiders, leafhoppers, psyllids, whiteflies and other pests, and can effectively inhibit the growth of pest populations in forests, nurseries, orchards, and farmland. As early as the 1960s, the United States used common lacewings to control cotton Spodoptera frugiperda. At present, the factory production and reproduction of lacewings have been successfully implemented. According to the research of the United States, in developed countries such as the United States and the United Kingdom, various types of lacewings have been sold. There are few reports and studies on the use of Lacewings in the family Neuroptera for biological control. Lacewings also have great potential in biological control. The main reasons are in three aspects: first, both adults and larvae have a large predation. , can prey on many kinds of pests, such as the eggs and adults of aphids, mites, scale insects, psyllids and other small soft insects; second, the larvae are sensitive, fast, and have strong predation ability; third, the adult lifespan Long and strong reproductive ability, diapause under low temperature conditions, and continue to lay eggs for reproduction after the conditions are suitable. Neuroptera is a very good resource for biological control, but Lacewings are still blank in this regard. The inventor, after a lot of research experiments during his Ph.D., proposed the use of aphids to feed the Lacewings ( Micromus angulatus Stephens). The laboratory method, this breeding method is not ideal in terms of egg production, egg hatching rate, larval predation, adult emergence rate, etc. The inventor further optimized the breeding method and proposed the use of aphids for breeding. The method of the brown flies provides technical support for the large-scale reproduction of the brown flies.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide a method for breeding brown lacewings by using aphids, and the soybean aphid ( Aphis glycines Matsumura) is used to reproduce the micromus angulatus Stephens.

In order to achieve the above object, the present invention proposes the following technical scheme: a method for utilizing aphids to breed lacewings, which is characterized in that, comprising the following steps: (1) feeding the aphids, (2) breeding the lacewings, and (3) packing;

Preferably, described aphid feeding comprises the following steps:

(1) Select soybean seedlings with 2-3 true leaves as the host plant of soybean aphid ( Aphis glycines Matsumura);

(2) The soybean seedlings with 2-3 true leaves were moved to the aphid breeding room, and each soybean seedling was inoculated with 3-6 adult soybean aphids. The temperature in the aphid breeding room was kept at 24-25 °C, and the humidity was 70%-80 %;

Preferably, described lacewing breeding comprises the following steps:

(a) The female brown lacewing adults to be laid eggs are moved into the rearing box, and the soybean aphids raised in step (1) are put into the rearing box together with the branches and leaves of the soybean seedlings, and the amount of each brown lacewing adult feeding the soybean aphid is: 30-80 heads/day;

(b) After the female lacewing adults start to lay eggs, move the collected eggs into an empty rearing box, and when the eggs hatch, put the soybean aphids raised in step (1) together with the branches and leaves of soybean seedlings into the rearing box, The amount of soybean aphid fed to each brown lacewing larva is 20-80 heads/day;

(c) After 8-13 days of rearing, the larvae of brown lacewings emerge into adults;

(d) The steps (a), (b) and (c) are carried out in a greenhouse. The greenhouse is set to 5 cycles for 24 hours a day, and the first cycle maintains a temperature of 24°C, a humidity of 45%, and a light intensity of 15Lx 2 hours, the second cycle maintains temperature 25℃, humidity 40%, light intensity 30Lx 4 hours, the third cycle maintains temperature 26℃, humidity 35%, light intensity 40Lx 6 hours, the fourth cycle maintains temperature 24℃, Humidity 45%, light intensity 15Lx 4 hours, the fifth cycle maintains temperature 23°C, humidity 55%, light intensity 0Lx 8 hours;

Preferably, the rearing box is a transparent plastic box, with a length of 20-24cm, a width of 12-15cm and a height of 7-10cm; half of the box cover is reserved, and the other half is covered with gauze, and the aperture of the gauze is 0.05-0.15 mm;

Preferably, the sub-packing comprises the following steps: The brown lacewing adults are sub-packed using a rearing box, and the brown lacewing larvae, eggs and pupae are sub-packed in glass tubes;

Preferably, the brown lacewing is Micromus angulatus Stephens;

The purpose of the breeding of the present invention is to prevent and control aphids on greenhouse flowers.

The beneficial effects of the invention are as follows: the optimized breeding conditions of the invention significantly improve the egg laying quantity, egg hatching rate, larval predation rate and adult emergence rate of P. Provided technical support.

Detailed ways

The technical solutions of the embodiments of the present invention are described below:

Example 1

1. Test method: (the test was repeated three times)

(1) Move 50 trays of soybean seedlings with 2-3 true leaves to the aphid breeding room. Each soybean seedling is inoculated with 5 adult soybean aphids. The temperature in the aphid breeding room is kept at 24°C and the humidity is 75%. Backup after 7 days;

(2) The female Lacewings hornbeam adults to be laid eggs are moved into the rearing box, which is a transparent plastic box, 20 cm long, 12 cm wide, and 7 cm high; half of the box cover is reserved, and the other half is covered with gauze, the yarn The aperture of the net is 0.15mm; the soybean aphid raised in step (1) is then put into the rearing box together with a small amount of soybean seedling branches and leaves, and the amount of soybean aphid fed to each brown lacewing adult is 30-45 head/day, and the preparation 30 rearing boxes, each of which contains 1 adult female Lacewings lacewings to be laid;

(3) After the female P. japonica adults started to lay eggs, the collected eggs were moved into the empty feeding box, and the number of eggs laid by each female P. japonica adults was counted; after 3.8 days of breeding, the eggs began to hatch ( Count the hatching rate of eggs), put the soybean aphids raised in step (1) together with the branches and leaves of soybean seedlings into the rearing box, and the amount of soybean aphid fed to each larvae of the brown lacewing is 20-70 heads/day, and the statistics 1- The daily prey amount of the 3rd instar larvae;

(4) After 9.2 days of rearing, the larvae of the brown lacewing began to emerge into adults, and the adult emergence rate was counted;

(5) The steps (2), (3) and (4) are carried out in a greenhouse. The greenhouse is set to 5 cycles 24 hours a day, and the first cycle maintains a temperature of 24°C, a humidity of 45%, and a light intensity of 15Lx 2 hours, the second cycle maintains temperature 25℃, humidity 40%, light intensity 30Lx 4 hours, the third cycle maintains temperature 26℃, humidity 35%, light intensity 40Lx 6 hours, the fourth cycle maintains temperature 24℃, Humidity 45%, light intensity 15Lx 4 hours, the fifth cycle maintains temperature 23°C, humidity 55%, light intensity 0Lx 8 hours;

(6) Parallel control settings, adjust step (5) to: "Steps (2), (3), (4) are carried out in the greenhouse, and the greenhouse is set to 4 cycles 24 hours a day, and the first cycle is maintained. Temperature 25.5°C, humidity 45%, light intensity 20Lx 3 hours, the second cycle keeps temperature 27°C, humidity 35%, light intensity 40Lx 10 hours, and the third cycle keeps temperature 26°C, humidity 45%, light intensity 20Lx 3 In the fourth cycle, the temperature was kept at 25°C, the humidity was 60%, and the light intensity was 0Lx 8 hours", and then the above steps (1), (2), (3), (4), (5) were repeated to obtain control 1; Adjust step (5) to: "steps (2), (3), (4) are carried out in a greenhouse, the temperature in the greenhouse is 25°C, the humidity is 50%, 12 hours of light, 12 hours of darkness", and then repeat the above steps (1), (2), (3), (4), (5), control 2 was obtained.

Table 1 The number of eggs laid by P. hornbeam under different breeding conditions

Note: In Table 1, the number of eggs laid by female adults of P. hornbeam is the average of three repeated experiments.

The data in Table 1 shows that: the optimization of the breeding conditions of the present invention increases the egg production of P. hornbeam.

Table 2 Hatching rates of Lacewings hornwort eggs under different breeding conditions

Note: The hatching rate of the eggs of P. hornbeam in Table 2 is the average of three repeated experiments

The data in Table 2 shows that the optimization of the breeding conditions of the present invention can significantly improve the hatching rate of the eggs of P. hornbeam.

Table 3 Predation amount of brown lacewing larvae under different breeding conditions

Note: In Table 3, the prey amount of the 1-3 instar larvae of the horned lacewing is the average of three repeated experiments

The data in Table 3 show that: the optimization of the breeding conditions of the present invention can increase the predation amount of the larvae of Lacewings.

Table 4 Emergence rate of P. hornbeam under different breeding conditions

breeding conditions Feather rate (%) this invention 98.3 Control 1 90.6 Control 2 69.5

Note: The eclosion rate of P. hornwort in Table 4 is the average of three repeated experiments

The data in Table 4 show that: the optimization of the breeding conditions of the present invention can significantly improve the emergence rate of the brown lacewing.

Claims (5)

1. a method for utilizing aphids to breed brown lacewings, is characterized in that, comprises the following steps: (1) aphid breeding, (2) brown lacewings breeding, (3) packing; Described aphid breeding may further comprise the steps: (1) Select soybean seedlings with 2-3 true leaves as the host plant of soybean aphid ( Aphis glycines Matsumura); (2) The soybean seedlings with 2-3 true leaves were moved to the aphid breeding room, and each soybean seedling was inoculated with 3-6 adult soybean aphids. The temperature in the aphid breeding room was kept at 24-25 °C, and the humidity was 70%-80 %; Described lacewing breeding comprises the following steps: (a) The female brown lacewing adults to be laid eggs are moved into the rearing box, and the soybean aphids raised in step (1) are put into the rearing box together with the branches and leaves of the soybean seedlings, and the amount of each brown lacewing adult feeding the soybean aphid is: 30-80 heads/day; (b) After the female lacewing adults start to lay eggs, move the collected eggs into an empty rearing box, and when the eggs hatch, put the soybean aphids raised in step (1) together with the branches and leaves of soybean seedlings into the rearing box, The amount of soybean aphid fed to each brown lacewing larva is 20-80 heads/day; (c) After 8-13 days of rearing, the larvae of brown lacewings emerge into adults; (d) The steps (a), (b) and (c) are carried out in a greenhouse. The greenhouse is set to 5 cycles for 24 hours a day, and the first cycle maintains a temperature of 24°C, a humidity of 45%, and a light intensity of 15Lx 2 hours, the second cycle maintains temperature 25℃, humidity 40%, light intensity 30Lx 4 hours, the third cycle maintains temperature 26℃, humidity 35%, light intensity 40Lx 6 hours, the fourth cycle maintains temperature 24℃, The humidity was 45%, the light intensity was 15Lx 4 hours, and the temperature was kept at 23°C, the humidity was 55%, and the light intensity was 0Lx 8 hours in the fifth cycle. 2. the method for utilizing aphids to breed brown flies according to claim 1, is characterized in that, described rearing box is transparent plastic box, length 20-24cm, width 12-15cm, height 7-10cm; , the other half is covered with gauze, and the aperture of the gauze is 0.05-0.15mm. 3. the method that utilizes aphids to breed brown flies according to claim 1, it is characterised in that the packaging comprises the following steps: The brown flies adults are packed in a rearing box, and the brown flies larvae, eggs and pupa are packed in glass tubes . 4. The method according to claim 1, wherein the brown flies is Micromus angulatus Stephens. 5. The purposes of the lacewing lacewing according to claim 4 for preventing and controlling aphids on greenhouse flowers.