CN106359314A - Method for mass propagation of harmonia axyridis by utilizing formula of alternative food and application - Google Patents

Abstract

The present invention relates to a method and application of a large-scale propagation of Heterochromia using a formula of alternate food, and discloses a method of mass-propagating Heterochromia using the eggs of Mediterranean mealylorrhizae combined with pig liver pulp as the basic feed. In 18 Under the condition of ‑32℃, they can develop normally, gain weight, prolong the spawning period and lifespan, and increase the number of eggs laid, up to 5004 eggs, far exceeding the previous artificial feed or other natural prey. The food in the method is easy to preserve and has high utilization rate, and it is easy to use with other artificial feed or natural prey at the same time to further reduce the cost. The invention is easy to use and has high yield.

Description

Method and use for mass breeding of ladybugs with an alternate food formula

technical field

The present invention relates to the method and application of a large number of propagation of heterochromia ladybugs with a formula of alternate food.

Background technique

As an excellent pest hunter, Heterochromia has the advantages of a wide range of predation, a large number of prey, a long lifespan, and strong adaptability. It has broad prospects in the biological control market of pests. The feeding and breeding of natural enemies is the key to biological control. Scientists have also conducted a lot of research on the mass reproduction of Heterochromia and artificial feed. However, after research on various artificial feeds, it was found that due to nutritional deficiencies and other reasons, problems such as low food intake, unhealthy individual development, long pre-oviposition period, no oviposition or low oviposition occurred, and it was necessary to add feeding stimulation factors. and gonadotropins. And the physical properties of the artificial feed will also affect the feeding. For example, the feed based on pig liver-sucrose is fluid, which is not easy for ladybugs to feed and is also easy to agglomerate and mold. The powder made after high temperature drying such as bee pupa affects the loss. Therefore, finding a feed that is easy to obtain, suitable for feeding, and can make the individual develop normally and lay eggs is the key to the large-scale reproduction of the heterochromia.

Contents of the invention

Contents of the invention

The task of the present invention is to provide a method and application of multiplying the heterochromia ladybug with a formula of alternating food. The technical scheme adopted in the present invention is: the method for multiplying the heterochromia in large quantities by using a formula of alternate food in the present invention, the formula of which alternate food is to combine the eggs of Mediterranean mealylosia moth and pig liver pulp.

There are 3 processing modes for the Mediterranean mealworm ovum of the present invention:

1. Fresh eggs (stored directly in a sealed box and in a refrigerator at 4°C, and used up within 3 days);

2. Ultraviolet treatment of eggs (put the plastic plate in the refrigerator for 2 minutes, take it out and place it in the air, sprinkle the eggs of the Mediterranean mealworm after the surface is moistened, spread it evenly on the plastic plate in a single layer, and put it on the plastic plate. 30-40 minutes on a 30cm countertop under a 20W UV lamp, then collect the eggs, store them in a sealed box in a refrigerator at 4°C, and use them up within 7 days);

3. Eggs are frozen (after UV treatment, store them in a refrigerator at -18°C to -20°C or take fresh eggs and store them in a refrigerator at -18°C to -20°C for more than 24 hours, and frozen eggs can be stored for 2 months).

The pig liver slurry of the present invention is made in the following way: fresh pig liver, sucrose and honey are mixed in a ratio of 5:1:1 by weight, and 1% of the total weight of olive oil is added; fresh pig liver is cut into pieces, mixed with honey, sucrose Put them together in a blender and stir until the pork liver is beaten to a pulp and the sucrose is completely melted, then add olive oil and stir well. After the pig liver pulp is prepared, pack it in a 100ml plastic box with a cover, store it in a refrigerator at -18°C to -20°C, and keep it for 1 to 2 months; On the corrugated paper, distribute the pork liver pulp evenly in the grooves of the corrugated paper, then dry it in a 45°C oven for 2 hours, then store it in a -18°C ~ -20°C refrigerator, and cut it into small pieces when using Just feed the heterochromatic ladybug with the card.

The method for multiplying the heterochromia in large quantities with a formula of alternating food of the present invention is to raise and make it mass-propagate the heterochromia with the mediterranean mealybug ova and pig liver pulp:

When feeding, in the 1st and 2nd instars of the ladybug, only the eggs of the Mediterranean mealybug were fed, and the 3rd, 4th and adult stages were fed with the eggs and pig liver pulp cards to provide sufficient feeding amount.

According to the method for multiplying the heterochromia in large quantities by using a formula of alternating food, the conditions for breeding the heterochromia are: 18-32°C, 50-85% relative humidity, and more than 8 hours of light.

In response to the needs of the prior art, the inventor has screened out the eggs of the Mediterranean mealybug moth plus pig liver pulp through a large number of tests, which can be used as feed for the mass reproduction of the heterochromia, and the feed can make the heterochromia normal growth and development, And prolong the spawning period, life expectancy, and increase the amount of eggs laid. The heterochromatia bred by this method has strong individuality and strong predation ability, and the hatching rate of the offspring exceeds 90%. The basic feed is based on the eggs of the Mediterranean mealybug moth and pig liver pulp, which is easy to store, has a high utilization rate, has no residue, is easy to raise indoors, and is not affected by the environment. It can also further reduce costs by adding other auxiliary feeds, which has significant economic benefits. , Social benefits.

detailed description

The present invention is further described below.

The method for multiplying the heterochromia in large quantities by using a formula of alternate food of the present invention, the formula of the alternate food is that the eggs of the mediterranean leaf spot borer cooperate with pig liver pulp.

There are 3 processing modes for the Mediterranean mealybug borer ovum of the present invention:

1. Fresh eggs (store directly in a sealed box and store in a refrigerator at 4°C, and use up within 3 days);

2. Eggs treated with ultraviolet rays (put the plastic plate in the refrigerator for 2 minutes, take it out and place it in the air, sprinkle the eggs of Mediterranean mealylosia moth on the surface after the surface is moistened, spread it evenly on the plastic plate in a single layer, and place it on the plastic plate. 30-40 minutes on a 30cm countertop under a 20W UV lamp, then collect the eggs, store them in a sealed box in a refrigerator at 4°C, and use them up within 7 days);

3. Eggs are frozen (after ultraviolet treatment, store them in a refrigerator at -18°C to -20°C or take fresh eggs and store them in a refrigerator at -18°C to -20°C for more than 24 hours, and frozen eggs can be stored for 2 months).

The pig liver slurry of the present invention is made in the following way: fresh pig liver, sucrose and honey are mixed in a ratio of 5:1:1 by weight, and 1% of the total weight of olive oil is added; fresh pig liver is cut into pieces, mixed with honey, sucrose Put them together in a blender and stir until the pork liver is beaten to a pulp and the sucrose is completely melted, then add olive oil and stir well. After the pig liver pulp is prepared, pack it in a 100ml plastic box with a cover, store it in a refrigerator at -18°C to -20°C, and keep it for 1 to 2 months; On the corrugated paper, distribute the pork liver pulp evenly in the grooves of the corrugated paper, then dry it in a 45°C oven for 2 hours, then store it in a -18°C ~ -20°C refrigerator, and cut it into small pieces when using Just feed the heterochromatic ladybug with the card.

The method for multiplying the heterochromia in large quantities with a formula of alternating food of the present invention is to raise and make it mass-propagate the heterochromia with the mediterranean mealybug ova and pig liver pulp:

When feeding, the 1st and 2nd instars of Heterochromia were only fed with the eggs of Trichomonas medidae, and the 3rd, 4th instars and adult stages were fed with the eggs of Trichomonas medidae and pig liver pulp, so as to provide sufficient feeding amount.

The daily amount of eggs of different ages of ladybug H. heterochromia is: 0.02g for the 1st instar, 0.025g for the 2nd instar, 0.025g for the 3rd instar, 0.06g for the 4th instar, 0.06g for adults when laying eggs, and 0.06g for adults when they are not laying eggs. 0.02g for adults, 7-10g for an average ladybug. The 3rd, 4th instar and adult Heterochromia basically eat about 0.02g of pig liver pulp every day.

Tests have shown that in the method for multiplying Heterochromia in large quantities with a formula of alternating food, the conditions for breeding Heterochromia are: 18-32°C, 50-85% relative humidity, and more than 8 hours of light.

The three treatments (fresh eggs + pig liver pulp; UV-treated eggs + pig liver pulp; frozen eggs + pig liver pulp) could make the ladybug grow normally.

Tests show that the survival rates of eggs, 1st, 2nd, 3rd, 4th, and pupal stages are 82% (damaged when picking eggs), 82%, 82%, 80%, 80%, 78%, and eclosion rate 75%.

Tests have shown that the developmental duration of female and male heterochromia is 17.44 days and 18.23 days respectively. After mating, the average lifespan of female adults is 137 days, the oviposition period is 106 days, and the average oviposition is 2775;

The body weight of newly emerged adults is female: 0.042g, male: 0.029g.

At 20-32°C, Heterochromia can complete development normally: the developmental period of female and male Heterochromia is 17.44 days and 18.23 days respectively, the egg-laying period is long, an average of 106 days, and the life span is also long, 137 days , the amount of eggs laid is 1-2 times more than other artificial feeds or even natural prey (aphids) in the past, with an average of 2775 eggs and a maximum of 4994 eggs.

The method of raising and making it a large number of multicolored ladybugs by coordinating pig liver pulp with the ovum of Trichomoner mediterraneae, the formula of the alternate food (the ovum of Trichomoner mediterranea cooperating with pig liver pulp) can be combined with other artificial feeds such as: Honey, pollen and other formula feeds or natural prey such as: aphids, moth eggs are also used to raise and reproduce Heterochromia in large numbers.

According to the method for mass-propagating ladybugs by using the eggs of the mealybug borer as the base feed and adding pig liver pulp, the ladybugs produced by the method can be used as natural enemies of pests and mites in crop production.

The above descriptions are only examples of the present invention, and all equivalent changes and modifications made according to the scope of the patent application of the present invention shall fall within the scope of the present invention.

Claims (4)

1. the method for a large number of multicolored ladybugs breeding with a formula of alternate food, it is characterized in that, the formula of described alternate food is Mediterranean mealiasis moth ovum and pig liver slurry; There are 3 ways to deal with the eggs of Mediterranean mealybug borer: a. Fresh eggs are directly stored in a sealed box and placed in a refrigerator at 4°C, and used up within 3 days; b. Ultraviolet treatment of eggs: put the plastic plate in the refrigerator for 2 minutes, take it out and place it in the air. After the surface is moistened, sprinkle the Mediterranean mealylosia moth eggs, make it evenly spread on the plastic plate in a single layer, and put it on the plastic plate. 30-40 minutes on a 30cm countertop under a 20W UV lamp, then collect the eggs, store them in a sealed box in a refrigerator at 4°C, and use them up within 7 days; c. Frozen eggs: after ultraviolet treatment, store them in a refrigerator at -18°C to -20°C or take fresh eggs and store them in a refrigerator at -18°C to -20°C for more than 24 hours; Pork liver pulp is made in this way: Fresh pork liver, sucrose and honey are mixed in a ratio of 5:1:1 by weight, and 1% of the total weight of olive oil is added; fresh pork liver is cut into pieces and put together with honey and sucrose Stir in a blender until the pork liver is beaten into a slurry and the sucrose is completely melted, then add olive oil and stir well; after the pork liver slurry is prepared, pack it in a 100ml plastic box with a cover, and store it at -18℃～-20℃ Store in the refrigerator; when in use, take out the pork liver pulp and thaw it in advance, brush the pig liver pulp on the corrugated paper, so that the pig liver pulp is evenly distributed in the grooves of the corrugated paper, and then dry it in a drying oven at 45°C for 2 hours. Then store it in the refrigerator at -18℃～-20℃, cut it into small pieces and feed it to ladybugs; Use the above-mentioned Mediterranean mealybug ova and pig liver slurry to feed and make it to breed a large number of ladybugs. When feeding, only feed the Mediterranean mealybug eggs in the 1st and 2nd instars of the Mediterranean mealybug, 3, 4 Infancy and adult stages were fed with Mediterranean mealybug eggs and pig liver paste cards; The conditions for the reproduction of Heterochromia are: 18-32°C, 50-85% relative humidity, and more than 8 hours of light. 2. according to claim 1, with a kind of formula that alternates food to multiply the method for Heterochromia in a large number, it is characterized in that, the amount that each age stage of described Heterochromia gets food every day is : 0.02g for 1st instar, 0.025g for 2nd instar, 0.025g for 3rd instar, 0.06g for 4th instar, 0.06g for adults when laying eggs, 0.02g for adults when not laying eggs, and an average of 7-10g for 1 Heterochromia; 3. The food intake of the 4th instar and adult Heterochromia is about 0.02g per day. 3. the method for multiplying heterochromia in large quantities with a kind of formula of alternate food according to claim 1, it is characterized in that, the formula of described alternate food is mixed with honey, pollen and other formula artificial feed or aphids, moths The eggs of the natural prey are also used to raise and reproduce the Heterochromia in large numbers. 4. according to claim 1, the method for a large number of breeding of ladybugs with a formula that alternates food, is characterized in that, the ladybirds produced by the method are used as pests, natural enemies of pest mites in the production of agricultural crops superior.