CN104322461B - A kind of method that pink rice borer indoors artificial is raised - Google Patents

Abstract

The present invention relates to a kind of method that pink rice borer indoors artificial is raised, adopt sugarcane, corn and wild rice stem are as artificial feed, in the metastable culturing room of temp. and humidity, be incubated in plastic culture bottle, with sugarcane, corn, wild rice stem is as artificial feed, within 4-5 days, change a feed, change feed method simple, within larva 30-45 days, cocoon can be changed, within 5-8 days, sprout wings, adult is transferred in large blake bottle, mix with 10-20 head adult 1:1, unrestricted choice mating, feed with 1-3% hydromel, collect the pieces of an egg on paraffin paper every day, pieces of an egg can be stored in 15-20 days in 4-10 DEG C of refrigerator, until worm's ovum reach a certain amount of after, test can be directly used in, the present invention can improve the efficiency that pink rice borer is raised greatly, pink rice borer robust growth, growth cycle is short, cocoon is heavy near with natural conditions spoiled cocoon heavy phase, eclosion rate is high, and Adult worms producting eggs amount is large, the incubation rate high of pieces of an egg, have high efficiency, can how in generation, raises continuously, quality of ootheca is excellent, the method is suitable for a large amount of productions of pink rice borer test worm and pieces of an egg.

Description

A kind of indoor artificial breeding method of C.

technical field

The invention relates to an indoor artificial breeding technique of S. chinensis, in particular to a method for indoor artificial breeding of S. chinensis.

Background technique

Sesamiainferens (Walker) belongs to the order Lepidoptera, Noctuidae, and the genus Stemborer. Mainly distributed in Asia, it is an omnivorous insect and an important pest of sugarcane, rice, corn, wheat, rape, sorghum and other grass weeds. As early as the 1950s, my country carried out related research on S. The artificial rearing technique of S. chinensis is a basic method for the study of S. sinensis, and the mature artificial rearing technique is an important technique for carrying out various insect experiments. This method adopts sugarcane, corn and wild rice stem as artificial feed, raises them in a culture room with relatively constant temperature and humidity, and replaces the feed every 4-5 days. The method of changing feed is simple, and the larvae can transform into cocoons in 30-35 days and emerge in 5-8 days. , transfer the adults to a large culture bottle, mix 10-20 adults with a male-to-female ratio of 1:1, freely choose to mate, feed with 1-2% honey water, collect egg masses on wax paper every day, and the egg masses can be stored at 4 Store in -10°C refrigerator for 15-20 days, and use them directly for testing after the eggs reach a certain amount. The invention can greatly improve the feeding efficiency of the large borer for many generations. The large borer grows robustly, has a short growth period, the cocoon weight is similar to the cocoon weight under natural conditions, the eclosion rate is high, the adult eggs are large, and the hatching rate of the egg mass is high. Efficiency, multi-generational continuous feeding, and excellent egg mass quality. This method is suitable for the large-scale reproduction of insects used in the test of C. Studies on toxicology, nutritional physiology and drug resistance physiology of S. moer have provided a large number of experimental insects, which will further provide more scientific basis for the control of S. moer.

Contents of the invention

The purpose of the present invention is to provide a method for indoor artificial breeding of S. moth, which utilizes plant feed to raise S. moth, and obtains a large amount of insect sources through multi-generation breeding. High, can meet the needs of various biological testing test insects.

The present invention is achieved through the following technical solutions:

A kind of method for indoor artificial breeding of C. chinensis comprises the following steps:

(1) Select dead heart seedlings in the sugarcane field, use a thin sickle to cut the stem tip meristem of the seedlings to obtain the larvae and cocoons of the stem borer, and find egg masses from the leaf tips of the sugarcane or trap adults of the stem borer with a black light as the source of insects;

(2) Conditions of the cultivation room: The cultivation chambers of larvae, cocoons, adults and egg masses all use sodium hypochlorite solution with a mass fraction of 0.1-0.2% to spray and disinfect the ground and bench of the cultivation chamber; the daytime temperature is 26±2°C , the humidity is 60-80%, the temperature at night is 22±2°C, the humidity is 80-90%, day:night is 16h:8h, good ventilation is required;

(3) Place the egg mass collected in step (1) in a well-sealed 100mL plastic culture bottle with a filter paper vent, keep the temperature at 26±2°C, and keep the humidity at 80%-90%. After 3- After 4 days, most of the eggs have entered the blackhead stage, put a few thin slices of water bamboo, and after another 1-2 days, wait for the eggs to hatch;

(4) Put the larvae collected in step (1) into 100mL sterilized plastic culture bottles with filter paper vents, and raise 1-3 heads/bottle. The young leaves of sugarcane, corn kernels and Zizania as feed, feeding in rotation; after the larvae cocoons, take out the cocoons in time and store them in a clean culture bottle for 5-7 days until the cocoons emerge;

(5) Mix 10-20 adult worms in step (1) and/or step (4) according to the ratio of male to female at 1:1 and centrally raise them in a 1500-2500mL culture bottle to allow them to mate freely. 3-5 cotton balls in honey water, stick 2 sheets of 5cm×5cm wax paper on the wall of the bottle, for the eggs of the adult Ceratosphaera moth, collect the eggs every day and replace the culture bottle, the eggs are sterilized with 75% ethanol for 30 seconds, and air-dried Surface ethanol, wait for it to hatch;

(6) The larvae hatched in step (3) or step (5) can be used for bioassay experiments, and the eggs or egg masses can be stored in a refrigerator at 4-10°C for 15-20 days, which can make the larvae After the eggs or egg masses reach a certain number, they hatch together again.

The optimal ratio of the type and total amount of feed for the larvae of S. chinensis is young leaves of sugarcane: corn kernels: wild rice stems = 1:1:3;

The volume ratio of honey and water fed to the adults of S. chinensis is honey:water=1-3:100.

The plastic culture bottle in the step (3) or (4) includes a bottle cap and a bottle body, the bottle cap and the bottle body are tightly closed by screwing caps, and a silicone sealing gasket (3) is placed between the bottle cap and the bottle body. The above-mentioned silicone sealing gasket (3) is circular, the diameter of the inner ring is 5.0cm, and the diameter of the outer ring is 5.5cm; a vent (1) with a diameter of 1cm is opened on the bottle cap and a plastic fiber film with a diameter of 1.5cm ( 2) Sealing; the body of the bottle is 4.0-6.0cm high, the upper diameter is 6.0cm, and the bottom diameter is 5.0cm; the bottle cap is provided with a groove with a width slightly wider than the silicone sealing gasket (3), just to seal the silicone The gasket (3) is put in to play the role of fixing the sealing gasket; the bottle cap and the bottle body are processed from PP plastic as a raw material, and the bottle body is transparent as a whole.

The present invention has the following outstanding substantive features and remarkable progress:

1) In the present invention, the ratio of sugarcane: corn: wild rice stem = 1:1:3 is used to feed the large borer, which greatly shortens the growth cycle of the large borer, and the larvae grow robustly. Production.

2) The composition and volume ratio of the honey water fed to the adults of S. chinensis is honey: water = 1-3: 100, which provides sufficient water for the adults of S. spp. A very necessary safeguard.

3) The present invention is to raise S. moth in a well-ventilated constant temperature and humidity culture room, control the length of light, so that S. moth can survive and develop better under controllable environmental conditions, and complete its generations. The development provides a strong guarantee and reduces the experimental error.

Description of drawings

Fig. 1 is the structural representation of special-purpose plastic culture bottle of the present invention;

In the picture: 1-air vent; 2-plastic fiber film; 3-silicone sealing gasket; 4-the groove on the bottle cap.

detailed description

The present invention is further described in detail through the following examples.

Example

(1) The sampling site is in the sugarcane area of Kaiyuan City, Yunnan Province. From April to May, select sugarcane plots that have not yet entered the jointing stage, look for dead heart seedlings from the sugarcane field, and use a thin inverted sawtooth sickle to cut the dead heart seedlings. Obtain a large number of larvae and cocoons from the shoot apical meristem; look for egg masses from the leaf tips of sugarcane; use black light lamps to trap adult larvae from 20:00 in the evening to 6:00 in the morning of the next day as an insect source.

(2) The number of larvae captured is 400-800, and the black light lamp traps about 50 adults; the cultivation chambers of the larvae, cocoons, adults and egg masses of the large borer are all sprayed with 0.1-0.2% sodium hypochlorite solution on the ground and bench of the cultivation chamber Disinfect surfaces. Cultivation room conditions: during the day, the temperature should be 26±2°C, and the humidity should be around 60-80%. At night, the temperature should be 22±2°C, and the humidity should be around 80-90%. During the day: 16h:8h at night, ventilation is required good;

(3) Place the egg mass collected in step (1) in a well-sealed plastic culture bottle of about 100 mL with a filter paper vent to prevent escape of newly hatched eggs. Keep the temperature at 26±2°C and the humidity at 85°C. %-95%, after 3-4 days, most of the eggs have entered the blackhead stage, put 3-4 slices of water bamboo, and after 1-2 days, wait for the eggs to hatch;

(4) Put the larvae collected in step (1) into 100mL sterilized plastic culture bottles with filter paper vents and raise 1-3 heads/bottle. As feed, feed in rotation. After the larvae cocoon, take out the cocoon in time and store it in a clean culture bottle for 5-7 days until the cocoon emerges;

(5) Mix 10-20 adult worms in step (1) and/or step (4) according to the ratio of male to female at 1:1 and centrally raise them in a 1500-2500mL culture bottle to allow them to mate freely. 3-5 cotton balls of honey water, stick 2 sheets of 5cm×5cm wax paper on the wall of the bottle, which is used for laying eggs of adults of the large borer, collect the eggs every day and replace the culture bottle, the eggs are sterilized by 75% ethanol for 30 seconds, Air-dry surface ethanol and allow to incubate.

(6) The hatched larvae can be used in bioassay experiments in time. The eggs or egg masses can be stored in a refrigerator at 4-10°C for 15-20 days. After reaching a certain number, they can be hatched together to facilitate experiments together.

Claims (5)

1. a method for pink rice borer indoors artificial raising, it is characterized in that, step is as follows:

(1) select withered heart seedling in sugarcane field, the shoot apical meristem using thin reaping hook to extract seedling obtains pink rice borer larva and cocoon, finds pieces of an egg or trap pink rice borer adult, as worm source with black light lamp from sugarcane blade tip;

(2) culturing room's condition: the liquor natrii hypochloritis that the culturing room of larva, cocoon, adult and pieces of an egg all utilizes mass fraction to be 0.1-0.2%, carries out surface spray sterilization to the ground of culturing room and stand; Day temperature is at 26 ± 2 DEG C, and humidity is at 60-80%, and evening, temperature was at 22 ± 2 DEG C, humidity at 80-90%, daytime: night is 16h:8h, requires well-ventilated;

(3) pieces of an egg collected in step (1) are placed in the 100mL plastic culture bottle with filter paper ventilating opening of good airproof performance, temperature remains on 26 ± 2 DEG C, humidity remains on 80%-90%, through 3-4 days, worm's ovum major part enters the blackhead phase, put into several pieces wild rice stem thin slices, then through 1-2 days, treat egg hatch;

(4) the pink rice borer larva of collecting in step (1) is placed in the plastic culture bottle that there be filter paper ventilating opening of 100mL through sterilizing, 1-3 head/bottle is raised, and using the young leaflet tablet of sugarcane, iblet and wild rice stem as feed, rotation is fed; After larvaization cocoon, cocoon is taken out in time and concentrates and deposit in 5-7 days in clean blake bottle, sprout wings to cocoon;

(5) the pink rice borer adult in 10-20 head step (1) and/or step (4) is raised in 1500-2500mL blake bottle by female-male proportion 1:1 mixing is concentrated, make its pangamy, 3-5 the cotton mass inhaled and have hydromel is put in bottle, bottle wall pastes 2 5cm × 5cm paraffin paper, for pink rice borer Adult worms producting eggs, collect worm's ovum every day and change blake bottle, worm's ovum was through 75% ethanol disinfection process 30 seconds, air-dry surperficial ethanol, treats that it is hatched;

(6) the pink rice borer larva hatched in step (3) or step (5) namely surveys experiment for raw.

2. the method for a kind of pink rice borer indoors artificial raising according to claim 1, it is characterized in that, the feed kind of described pink rice borer larva and the ratio of total amount are the young leaflet tablet of sugarcane: iblet: wild rice stem=1:1:3.

3. a kind of pink rice borer indoors artificial according to claim 1 method of raising, is characterized in that, the feed volume ratio of hydromel of described pink rice borer adult is honey: water=1-3:100.

4. the method for a kind of pink rice borer indoors artificial raising according to claim 1, it is characterized in that, described worm's ovum or pieces of an egg preserve 15-20 days in 4-10 DEG C of refrigerator.

5. the method for a kind of pink rice borer indoors artificial raising according to claim 1, it is characterized in that, the plastic culture bottle that described step (3) or (4) are selected comprises bottle cap and body, bottle cap and body cover tightly in spiral cover mode, a silica gel sealing packing ring (3) is padded between bottle cap and body, described silica gel sealing packing ring (3) is annular, and inner circle collar extension footpath is 5.0cm, and outer toroid bore is 5.5cm; Bottle cap opened the ventilating opening (1) of a diameter 1cm and seal with the plastic optical fibre film (2) of diameter 1.5cm; Described body, high 4.0-6.0cm, top bore 6.0cm, base diameter 5.0cm; Described bottle cap is provided with a groove, width is slightly wider than silica gel sealing packing ring (3), is just put into by silica gel sealing packing ring (3), plays the effect of fixing seal packing ring; Described bottle cap and body form using PP plastics as Raw material processing, body whole clearing.