CN103975901B - A kind of Chrysomya megacephala maggot cultivation residue material that utilizes lures the method collecting wild black soldier flies - Google Patents

Abstract

The invention provides a method for luring wild black soldier flies by using the leftover material from the cultivation of Chrysalis maggots. Specifically, it first uses bran and hemoglobin powder to cultivate the black soldier flies, and then uses the remaining materials from the cultivation to trap wild black soldier flies. Black Soldier Fly. The suitable period for trapping wild black soldier flies in the present invention is from April to October, when the temperature is 23-35° C., and the weather is clear and rainless. The effect of the present invention on trapping wild black soldier flies is very remarkable, especially the lysine rich in blood globulin powder is easily decomposed into cadaverine and other substances with strong irritating odor under the action of Chrysophylla maggots and natural microorganisms , It has a strong effect of luring and laying eggs on wild black soldier fly adults. After the wild black soldier fly eggs hatch and grow at room temperature, a large number of black soldier fly provenance can be obtained in a short time. The method is simple, convenient and effective, and can greatly save labor and time for obtaining wild black soldier fly provenance.

Description

A method for trapping wild black soldier flies by using leftover material from Chrysalis maggot breeding

technical field

The invention belongs to the technical field of black soldier fly artificial breeding. More specifically, it relates to a method for trapping wild black soldier flies by using the leftover material from the cultivation of Chrysalis maggots.

Background technique

The black soldier fly, scientific name Hermetiaillucens L., is a holometabolous insect belonging to the class Insecat (Insecat), Diptera (Diptera), Brachycera (Brachycera), Stratiomyidae (Stratiomyidae). Soldier flies ( Hermetia ). Adults have gray and black wings, degenerated mouthparts, body length 15-20mm, and body is mainly black. Eggs are about 1 mm in diameter and long oval. Native to America, it is widely distributed all over the world (between 40 degrees north and south latitude). The growth rate of black soldier fly larvae is relatively fast. Under the condition of about 28°C, the egg period of black soldier fly is 4 days, and the larval growth period takes about 20 days.

Black soldier fly is an important resource insect and environmental protection insect, which can be used to biotransform various organic wastes or livestock and poultry manure, and at the same time produce a large amount of high-quality organic fertilizer and black soldier fly insect protein. Because it is neither a sanitary pest nor an agricultural pest, and its conversion efficiency to organic waste is significantly higher than that of Diptera fly insects, its production technology is relatively simple, and its breeding cost is low, so it has attracted more and more attention in recent years. However, due to the low population density of black soldier flies in the wild, it is difficult for ordinary farmers to obtain a stable and large source of black soldier flies.

The existing technology is mainly to collect black soldier fly prepupa or pupa beside the dung pile of chicken farm or pig farm, and then breed and expand the population, or introduce or purchase provenance from relevant scientific research units. The above prior art has great defects, that is, obtaining the source of black soldier flies has greater uncertainty and instability, because chicken farms or pig farms usually remove the accumulated manure or use it for biogas preparation in about a week, wild Black soldier fly populations are extremely low.

Contents of the invention

The technical problem to be solved by the present invention is to overcome the defects and technical deficiencies in the artificial cultivation and provenance acquisition of existing black soldier flies, and provide a method for efficiently trapping wild black soldier flies by using the leftover material from the cultivation of Chrysophora chinensis.

The above object of the present invention is achieved through the following technical solutions:

The invention provides a method for trapping wild black soldier flies by using the leftover material from the cultivation of golden fly maggots, the steps are as follows:

S1. Obtain the surplus material of Chrysalis maggot cultivation;

S11. Prepare golden fly maggot compost: mix 60-95% bran and 5-40% hemoglobulin powder according to the mass percentage, add water to adjust the humidity to 60-65%;

S12. according to the ratio of inserting 0.5 to 1.2 g of Chrysophalis ova in every 1 kg of Chrysocephala compost, insert the Chrysocephala ovum into the cultivation container equipped with Chrysophyllum macrocephala compost;

S13. After 2 days of inoculation, the eggs of Chrysocephala chinensis began to hatch, and began to eat and transform the compost. After 4 to 5 days of cultivation, Chrysocephala maggots crawled away from the compost by themselves. At this time, the remaining compost in the culture container was It is the leftover material from the cultivation of Chrysalis macrocephala; the humidity of the compost needs to be maintained at 60-65% during the whole process, especially after 4-5 days of cultivation, water needs to be added, and the humidity of the compost should be adjusted to 65-70% (70% is the best). % humidity), because Chrysalis macrocephala likes to pupate in a dry environment, and meanwhile it is beneficial for Chrysophora macrocephala to crawl away from the culture basin by the surface tension of water. Preferably, a larger pot can be covered in the container for raising Chrysophalis maggots, and an appropriate amount of dry bran can be sprinkled into it, so as to facilitate the collection of Chrysophidae maggots separated;

As a solution, there are two breeding pots of different sizes inside and outside the breeding container. The culture material of Chrysalis macrocephala is placed in the small pot inside, and the maggots of Chrysalis macrocephala are inserted in proportion. After cultivating on the 4th night, there will be Chrysocephala maggots crawled away from the compost by themselves and fell into a large basin outside (covered with dry bran). Until the 5th day, the maggots were basically completely separated from the compost. At this time, the remaining compost in the culture container It is the leftover material from the culture of botch fly maggots. The bran is commercially available, and the content of specific components is not strictly limited.

S2. Utilize the leftover material from the culture of the golden fly maggot to lure the wild black soldier fly.

Preferably, the specific method for trapping wild black soldier flies described in S2 by utilizing the remaining material of Chrysophidae maggot cultivation is:

S21. Add water to the remaining material of Chrysalis macrocephala cultivation, and adjust the humidity to 70-75% by mass of water;

S22. Put the leftover material from the cultivation of Chrysalis macrocephala into a container with a cover (it is not completely sealed, there is a gap of 1-3 mm between the cover and the container, so that the wild black soldier flies can lay eggs in the gap, and store them outdoors at 23-35 °C. Stand in a cool and ventilated place for 10-15 days;

S23. Observe that black soldier flies fly to lay eggs or see a large number of black soldier fly larvae growing after removing the cover, that is, the provenance of wild black soldier flies is successfully attracted; move the container indoors to cultivate a strong population.

As a solution, you can put the leftover material of Chrysalis macrocephala breeding into plastic pots, place the plastic pots under the shade of trees, bushes or under the eaves, and cover a piece of wood or plastic board with an area slightly larger than the mouth of the plastic pot (it cannot be completely Sealed to make it easier for wild black soldier flies to lay eggs in the crevices), after the plastic basin was left to stand for 1 to 2 days, 1 to 5 black soldier flies could be found flying to lay eggs; after standing for 10 to 15 days, uncover the wooden board Or plastic board observation, if a large number of black soldier fly larvae are seen growing, that is, the wild black soldier fly provenance has been successfully lured.

Preferably, as described in S11, 80-90% bran and 10-20% hemoglobin powder are mixed.

Preferably, the humidity in S11 is adjusted to a moisture content of 60% by mass.

Preferably, in S12, 0.8-1.0 g of Chrysocephala eggs are added to each 1 kg of Chrysophydia botroe maggot compost.

Preferably, the humidity in S21 is adjusted to a moisture content of 70-72% by mass.

The temperature in the cool and ventilated place mentioned in S22 is 25-28°C.

The cultivation conditions described in S23 are the conventional breeding methods of black soldier flies. In the implementation process of the present invention, the cultivation conditions specifically adopted are: under the conditions of 25-28°C in the room, the cultivation using the formula of 30% bran and 70% water Whenever the culture material turns black, a part of the new culture material is supplemented until more than 95% of the black soldier fly larvae mature and blacken, and the supplementary feed is stopped. Generation Provenance.

Preferably, the suitable time for trapping wild black soldier flies according to the present invention is from April to October every year, when the temperature is 23-35° C., and the weather is clear and rainless.

In order to overcome the problem of the provenance of the existing artificial breeding of black soldier flies, the present invention utilizes bran and hemoglobin powder to breed Chrysalis maggots, obtains the remaining material of Chrysalis maggots, and then uses the remaining materials of Chrysalis maggots to lure Set of breeding wild black soldier flies. The hemoglobulin powder used is rich in lysine, which is easily decomposed into cadaverine and other substances with a strong pungent smell under the action of golden fly maggots and natural microorganisms, which is extremely attractive to wild black soldier fly adults The role of spawning. During the cultivation of golden fly maggots, maintain the humidity of the compost at 60-65%, especially after 4-5 days of cultivation, adjust the humidity of the compost to 65-70% (the best humidity is 70%) by adding water , to realize the self-separation of Chrysalis macrocephala and compost. Simultaneously, put a larger pot on the outer cover of the container for raising the golden maggot, and sprinkle into it an appropriate amount of dry chaff, so as to facilitate the collection of the separated golden maggot.

For the selection of the culture material of Chrysostrum botany, the inventor has carried out a large number of experiments and verifications and found that the bran and hemoglobin powder used have the best effect and cannot be simply replaced. First of all, for bran, the substrates of conventional fly breeding in the field also include chicken manure, pig manure, distiller's grains, etc., but they are not as convenient and easy to get as bran, and the breeding effect of Chrysalis macrocephala is not as good as bran. For blood globulin powder, in theory, protein substances rich in lysine can replace hemoglobulin powder. Currently available protein substances rich in lysine include soybean meal and fish meal, etc., but similarly, the invention After a large number of experiments, people found that the pungent smell it produces is not as strong as that of hemoglobin powder, and the trapping effect is not as good as that of hemoglobin powder.

Chrysomyamegacephala (Fabricius) is a holometabolous insect belonging to Diptera, Calliphoridae, Chrysomya . Adults are fat, with red compound eyes and green or blue body. They are widely distributed all over the world. Larval maggot type, in the wild state, mainly feeds on various waste animal matter or livestock manure, while in captivity, corn meal, fish meal and bran can be used as the main food. The life cycle of Chrysocephalus chinensis is very short. Under the condition of 28 ℃, the growth period of its larvae is 5 days. Because of the large population of Chrysocephala in the natural environment, the provenance of wild Chrysocephala can be obtained more easily and further cultivated into an artificial population.

The trapping method described in the present invention is easy to operate and has good effect. The water, bran, hemoglobin powder, and containers used are very common, low in cost, and do not need special measures such as sterilization. For example, water can It can be tap water, well water, river water, etc.

The present invention has the following beneficial effects:

The invention provides a method for luring wild black soldier flies by using the leftover material of Chrysalis maggot cultivation. The leftover material from golden fly maggot breeding is used to lure and breed wild black soldier flies. The method is very effective in trapping wild black soldier flies. The lysine rich in blood globulin powder is easily decomposed into cadaverine and other substances with strong pungent odor under the action of golden fly maggots and natural microorganisms. , It has a strong effect of luring and laying eggs on wild black soldier fly adults. After the wild black soldier fly eggs hatch and grow at room temperature, a large number of black soldier fly provenance can be obtained in a short time.

The leftover material of Chrysalis maggot breeding is no longer suitable for the growth of larvae of wild Chrysalis maggots or other flies because of the nutrition has been consumed by the raised Chrysalis maggots, but it can still be used by wild black soldier fly larvae, because black soldier fly larvae Soldier fly larvae have far lower nutritional requirements than fly insects, so that the purer wild black soldier fly provenance can be lured. At the same time, the rational control of conditions in the trapping method of the present invention further ensures the acquisition of black soldier flies. The purer source of wild black soldier flies.

The method of the invention is simple, effective and convenient to operate, can greatly save labor and time for obtaining wild black soldier fly provenance, and the trapping process does not need human manipulation, and the whole trapping process can be completed in only 19-20 days, which is different from the prior art In comparison, it is obviously time-saving, trouble-saving and labor-saving. The materials used are easy to obtain and the cost is low.

detailed description

The present invention will be further described below in conjunction with specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the technical field.

Unless otherwise specified, the reagents and materials used in the present invention are commercially available.

Example 1

Choose to lure wild black soldier flies in April of that year, when the temperature is 23-35°C, the weather is clear and there is little rain.

The specific steps are:

S1. Prepare golden fly maggot compost, the formula is: bran 60%+hemoglobulin powder 40%, add tap water, adjust the compost humidity to 60% (percentages are mass percent concentration);

S2. Insert 1.2 g of Chrysophyllum macrocephala eggs into every 1 kg of Chrysalis macrocephala compost, and maintain the humidity of the compost at 60%;

S3. Chrysocephala maggots hatched on the 2nd day of insertion, and began to eat and transform culture materials; after 4 to 5 days of growth, Chrysocephala maggots climbed out of the culture materials and fell into special collection basins. At this time, culture The rest of the compost in the pot is the rest of the culture of Chrysalis macrocephala;

One day before isolation, water was added to maintain the humidity of the compost at 68%.

S4. Add tap water to the remaining material of Chrysalis macrocephala cultivation, and adjust its humidity to 70% (mass percentage concentration);

S5. Place the plastic basin containing the leftover material of Chrysalis macrocephala breeding under the shade of trees, bushes or under the eaves, and cover it with a wooden or plastic board with an area slightly larger than the mouth of the plastic basin (it cannot be completely sealed to facilitate wild black water. Gadflies lay eggs in the crevices), and after the plastic basin was left to stand for 2 days, 3 black soldier flies were found to fly to lay eggs;

After standing still for 10 days, uncover the wooden board or plastic board, and find that a large number of black soldier fly larvae are growing, and bring them back to the room in pots to cultivate and expand the population, and successfully lure the wild black soldier fly provenance.

After observation and identification, the wild black soldier fly worms obtained by trapping are relatively fat, with an average weight of about 0.12g per head.

The trapping process does not need human manipulation, is convenient and simple, and the whole trapping process only takes 14 to 15 days, which is obviously time-saving, trouble-saving and labor-saving compared with the prior art.

Example 2

Choose to lure wild black soldier flies in October of that year, when the temperature is 23-35°C, the weather is clear and there is little rain.

The specific steps are:

S1. Prepare golden fly maggot compost, the formula is: bran 95%+hemoglobulin powder 5%, add tap water to adjust the compost humidity to 65% (percentages are mass percent concentration);

S2. Insert 0.5 g of Chrysophyllum macrocephala ovum into every 1 kg of Chrysalis macrocephala compost, and maintain the humidity of the compost at 65%;

S3. Chrysocephala eggs hatched on the 2nd day after inoculation, and began to feed and transform culture materials. After the golden fly maggot grows for 4 to 5 days, it climbs away from the culture material by itself and falls into a special collection basin. At this time, the remaining culture material in the culture basin is the remaining material for the culture of golden fly maggot;

One day before separation, add water to maintain the humidity of the compost at 70%.

S4. Add tap water to the remaining material of Chrysalis macrocephala cultivation, and adjust its humidity to 75% (mass percentage concentration);

S5. Place the plastic basin containing the leftover material of Chrysalis macrocephala breeding under the shade of trees, bushes or under the eaves, and cover it with a wooden or plastic board with an area slightly larger than the mouth of the plastic basin (it cannot be completely sealed to facilitate wild black water. Gadflies lay eggs in the crevices), and after the plastic basin was left to stand for 2 days, a black soldier fly was found to fly to lay eggs;

After standing still for 15 days, the wooden or plastic boards were uncovered, and a large number of black soldier fly larvae were found to be growing, and they were brought back indoors in pots to cultivate and grow the population, successfully attracting wild black soldier fly larvae.

After observation and identification, the wild black soldier fly larvae obtained by trapping are large in size, with an average weight of about 0.14g per head.

The trapping process does not need human manipulation, is convenient and simple, and the whole trapping process only takes 19-20 days. Compared with the prior art, it is obviously time-saving, trouble-saving and labor-saving.

Example 3

Choose to lure wild black soldier flies in July of that year, when the temperature is 23-35°C, the weather is clear and there is little rain.

The specific steps are:

S1. Prepare golden fly maggot compost, formula is: bran 85%+hemoglobulin powder 15%, add tap water to adjust the compost humidity to 63% (percentages are mass percent concentration);

S2. insert 0.8g of Chrysophora macrocephala ovum into every 1kg of Chrysophora macrocephala compost, and maintain the humidity of compost at 63%;

S3. Chrysocephala eggs hatched on the 2nd day after inoculation, and began to feed and transform culture materials. After the golden fly maggot grows for 4 to 5 days, it climbs off the compost by itself and falls into a special collection basin. This moment, the compost remaining in the culture pot is the culture surplus material of Chrysalis macrocephala.

One day before separation, add water to maintain the humidity of the compost at 70%.

S4. Add tap water to the remaining material of Chrysalis macrocephala cultivation, and adjust its humidity to 72% (mass percentage concentration);

S5. Place the plastic basin containing the leftover material of Chrysalis macrocephala breeding under the shade of trees, bushes or under the eaves, and cover it with a wooden or plastic board with an area slightly larger than the mouth of the plastic basin (it cannot be completely sealed to facilitate wild black water. Gadflies lay eggs in the crevices), and after the plastic basin was left to stand for 1 day, two black soldier flies were found to fly in;

After standing still for 13 days, the wooden or plastic boards were uncovered, and a large number of black soldier fly larvae were found to be growing, and they were brought back indoors in pots to cultivate and grow the population, successfully attracting wild black soldier fly larvae.

After observation and identification, the wild black soldier fly larvae obtained by trapping are large in size, with an average weight of about 0.13g per head.

The trapping process does not need human manipulation, is convenient and simple, and the whole trapping process only takes 17-18 days. Compared with the prior art, it is obviously time-saving, trouble-saving and labor-saving.

Claims (9)

1. a kind of method that utilizes golden fly maggot to cultivate remaining material lures wild black soldier fly, it is characterized in that, step is as follows: S1. Obtain the surplus material of Chrysalis maggot cultivation; S11. Prepare golden fly maggot compost: mix 60-95% bran and 5-40% hemoglobulin powder according to the mass percentage, add water to adjust the humidity to 60-65%; S12. Inject 0.5-1.2 g of Chrysalis macrocephala eggs per 1 kg of Chrysophyllum macrocephala compost, and maintain the humidity of the compost at 60-65%; S13. After cultivating for 4-5 days, adjust the humidity of the compost to 65-70% by replenishing water, and Chrysalis macrocephala crawls away from the compost by itself, and the remaining compost at this time is the remaining material for cultivating Chrysalis macrocephala; S2. Utilize the leftover material of the golden fly maggot breeding to lure wild black soldier flies; S21. Adding water to the remaining material of Chrysalis macrocephala cultivation, adjusting the humidity until the mass percentage of water is 70-75%; S22. Put the remaining material of Chrysalis macrocephala culture into a container with a cover, and put it in a cool and ventilated place outdoors and let it stand for 10-15 days; S23. Observe that black soldier flies fly to lay eggs or uncover black soldier fly larvae growing, that is, successfully attract wild black soldier fly provenance; move the container indoors to cultivate a strong population. 2. The method according to claim 1, characterized in that, the mass percentage of the bran in S11 is 80-90%, and the mass percentage of the blood globulin powder is 10-20%. 3. The method according to claim 1, characterized in that the humidity in S11 is adjusted to 60% by mass of the moisture. 4. The method according to claim 1, characterized in that 0.8-1.0 g of Chrysocephala eggs are inserted into every 1 kg of Chrysophyllum botroe maggot compost in S12. 5. The method according to claim 1, characterized in that, the humidity of the compost described in S13 is adjusted to 68-70%, and dry bran is placed around the outside of the container for raising Chrysophalis macrocephala. 6. The method according to claim 1, characterized in that, the humidity in S21 is adjusted to a mass percentage of water of 70-72%. 7. The method according to claim 1, characterized in that the temperature of the cool and ventilated place in S22 is 25-28°C. 8. The method according to claim 1, characterized in that, the outdoor cool and ventilated place in S22 is the shade of trees, bushes or under the eaves. 9 . The method according to claim 1 , characterized in that, the container with a lid described in S22 is not completely sealed, and there is a gap of 1-3 mm between the lid and the container.