CN108207809A - A kind of indoors artificial cultural method of carpocapsa pononella - Google Patents

Abstract

The invention discloses a kind of indoors artificial cultural method of carpocapsa pononella, the growth course of carpocapsa pononella is " ovum --- larva --- pupa --- adult ", and Population breeding is realized in the oviposition of adult post-coitum.Indoors artificial cultural method includes：Establish the interior space of the controllable suitable carpocapsa pononella growth of the environment such as a temperature, humidity and illumination；From field acquisition band worm apple, carpocapsa pononella worm sources are obtained, interior is taken back and is cultivated, form the indoor carpocapsa pononella population of subculture.The indoors artificial cultural method of the carpocapsa pononella of technical solution of the present invention, cultivation operating method is simple, and breeding environment is easily-controllable, and long-term cultivation can establish stable indoor carpocapsa pononella population, sufficient worm sources are provided, while the study on prevention for carpocapsa pononella provides condition for follow-up carpocapsa pononella correlative study.

Description

A kind of indoor artificial breeding method of codling moth

technical field

The invention relates to an indoor artificial cultivation method of codling moth.

Background technique

The codling moth Cydia pomonella (L.) is native to Europe and is a world-renowned important invasive pest that seriously harms apples and pears, and is also an important foreign quarantine object in my country. It has now spread to Xinjiang, Gansu, Inner Mongolia, Ningxia, Heilongjiang, Jilin and other provinces and regions in my country, posing a serious threat to the safe production of apples in our country.

Codling moth control research requires a large number of adults and larvae. The most primitive source of these adults and larvae is mostly wild capture, but codling moth mostly grows in apples. After pupation, adults have a short lifespan and are difficult to capture, and capture It will die due to changes in the environment, which seriously hinders the research team's research on codling moths. In order to solve these problems, people began to study the indoor artificial breeding of codling moth to establish an indoor codling moth population and provide insect sources for follow-up research.

Contents of the invention

The purpose of the present invention is to provide an indoor artificial breeding method of codling moth, which is simple, can establish an indoor codling moth population and provide insect sources for follow-up research.

The technical solution of the present invention is an indoor artificial cultivation method of codling moth, the growth process of the codling moth is "egg-larvae-pupa-adult", and the adults lay eggs after mating to realize reproduction. Indoor artificial cultivation method comprises the following steps,

(1) Establishment of codling moth breeding environment

Choose a separate breeding room. The breeding room is equipped with windows that can be opened and closed, and a separate sealable breeding room is established in the breeding room. Air conditioners, humidifiers, lighting, ultraviolet lights, temperature sensors, and humidity are installed in the breeding room. Sensors, and set up a breeding platform and racks in the breeding room, on which are placed net bags, corrugated paper, egg paper, centrifuge tubes, absorbent cotton, toothpicks, egg cartons, artificial climate boxes, egg-laying boxes, Purified water, sodium hypochlorite disinfection water, honey water, special feed, petri dishes, and all the above items are sterilized and then placed in the breeding room, and the spawning box is placed in the artificial climate box;

(2) Collection of codling moth eggs

Put the moth fruits collected from the field with codling moth larvae into mesh bags, and place them in a breeding box to raise them. Keep the temperature T (25 ± 1) °C, humidity RH (70 ± 5)% and Photoperiod L:D=16:8h, put some corrugated paper into the net bag at the same time, lure the larvae to cocoon and pupate, after the pupae emerge into adults, collect them into the spawning box, supply honey water with a concentration of 10%, and produce Single-sided egg-covered paper in the egg box lures adults to lay eggs, and the egg paper is replaced every 2-3 days according to the amount of eggs laid by adults, and the date of replacement egg paper is recorded, and then the replaced egg paper is placed in the egg paper with built-in moist absorbent cotton box, and put the egg carton into the artificial climate box to wait for the eggs to hatch. Before the eggs hatch, that is, the blackhead stage of the eggs, they should be disinfected with sodium hypochlorite water with a concentration of 1‰, rinsed with pure water for 2-3 times, and the larvae will emerge from the eggs;

(3) Indoor breeding of codling moth

Feed in sterilized centrifuge tubes, fill each centrifuge tube with special feed, connect the larvae to the centrifuge tube, plug the tube mouth with sterilized absorbent cotton, and put the larvae hatched on the same day into the same centrifuge tube, and Record the date, and then check the centrifuge tube once a week to remove dead insects in time to prevent bacteria and viruses from infecting other healthy larvae in the centrifuge tube. When the mature larvae pupate, collect the pupae and put them in a culture dish with moist absorbent cotton, and culture them The dishes are placed in the spawning box placed in the artificial climate box, and the temperature T (25 ± 1) °C, humidity RH (70 ± 5)%, and photoperiod L: D = 16: 8h are kept in the artificial climate box all the time. Its adults emerge, mate, lay eggs, and collect the eggs of the next generation, so that they are continuously reared to form an indoor codling moth population.

Preferably, the bored fruit collected from the field with codling moth larvae needs to be disinfected. The disinfection method is to use a disposable cloth towel soaked in sodium hypochlorite disinfectant to wipe the surface of the bored fruit, and then wipe it with a disposable cloth towel soaked in purified water. The surface of the bored fruit is sufficient. Both the disposable cloth towel soaked in sodium hypochlorite disinfectant and the disposable cloth soaked in purified water need to be wrung out to ensure that the disposable cloth is moist and free of dripping liquid, so as to prevent sodium hypochlorite disinfectant and purified water from entering the moth. internal.

Preferably, the volume of the centrifuge tube is 5mL, and the amount of special feed put into the centrifuge tube is 2mL.

Preferably, the petri dish is made of special feed for codling moth.

The technical scheme of the present invention relates to an indoor artificial breeding method for codling moths. The special feed raw materials mainly include whole wheat flour, soybean meal, yeast, sucrose, and agar, and are prepared by mixing, fermenting, cooking, and sterilizing.

Beneficial effects of the technology of the present invention:

The indoor artificial breeding method of codling moth according to the technical scheme of the present invention has simple breeding operation method, easy control of the breeding environment, and long-term breeding can establish an indoor codling moth population, which can provide insect sources for subsequent research on codling moth, and provide fruit for apples. Provide conditions for the prevention and control of silverfish moth.

Detailed ways

In order to facilitate those skilled in the art to understand the technical solution of the present invention, the technical solution of the present invention will be further described in conjunction with specific examples or implementation modes.

A kind of indoor artificial culture method of codling moth, comprises the following steps,

(1) Establishment of codling moth breeding environment

Choose a separate breeding room with windows that can be opened and closed, and build a separate sealable breeding room in the breeding room. Air conditioners, humidifiers, lighting, ultraviolet lights, temperature sensors, and humidity sensors are installed in the breeding room. It is used to control the temperature, humidity and light time in the breeding room in real time, and set up and operate the breeding platform and racks in the breeding room, and prepare sterilized mesh bags, corrugated paper, egg paper, centrifuge tubes, absorbent cotton, toothpicks in the breeding room , egg carton, artificial climate box, spawning box, purified water, sodium hypochlorite disinfection water, honey water, special feed, petri dish, and prepare multiple copies of the above items;

(2) Collect codling moth eggs

Pick bored fruit with codling moth larvae in the wild, and wipe the bored fruit surface with a disposable cloth towel soaked in sodium hypochlorite disinfectant, and then wipe the bored fruit surface again with a disposable cloth soaked in pure water to remove the bored fruit Put them into a net bag and place them in a breeding box for breeding. Keep the temperature in the artificial climate box at 24-26°C, humidity at 65-75%, and a photoperiod of 16 hours with light and 8 hours with darkness. Put some corrugated paper in the mesh bag , to lure the larvae to cocoon and pupate. After the pupae emerge into adults, they are collected into the artificial climate box and supplied with honey water with a concentration of 10%. The single-sided egg-covered paper in the egg-laying box lures adults to lay eggs. According to the amount of eggs laid by adults Replace the egg paper every 2-3 days, and record the date of replacing the egg paper. Then put the egg paper into the egg carton with moist absorbent cotton, and put the egg carton into the artificial climate box to wait for the eggs to hatch. Before the eggs hatch, the egg blackheads will appear. Regularly disinfect with sodium hypochlorite water with a concentration of 1‰ and rinse with pure water for 2-3 times until the eggs hatch and larvae emerge;

(3) Raising codling moth indoors

Use sterilized centrifuge tubes with a volume of 5mL to raise them, put 2mL of special feed in each centrifuge tube, connect the larvae to the centrifuge tube, plug the tube mouth with sterilized absorbent cotton, and receive the larvae hatched on the same day into the same centrifuge tube. In the centrifuge tube, and record the date, then check the centrifuge tube once a week to remove dead insects in time to prevent bacteria and viruses from infecting other healthy larvae in the centrifuge tube. When the mature larvae pupate, collect the pupae and put them in a petri dish with built-in moist absorbent cotton , and put the petri dish into the spawning box. This process is carried out in the artificial climate box, and the temperature T (25 ± 1) ° C, humidity RH (70 ± 5) %, and photoperiod L: D are always maintained in the artificial climate box. =16:8h, wait until the adults emerge, mate, lay eggs, and collect the eggs of the next generation, so that the indoor codling moth population is formed by continuous feeding.

The technical solution of the present invention has exemplarily described the invention above in conjunction with the embodiments. Obviously, the specific implementation of the present invention is not limited by the above-mentioned method, as long as various non-substantial improvements made by the method concept and technical solution of the present invention are adopted, Or directly applying the concept and technical solution of the invention to other occasions without improvement is within the protection scope of the present invention.

Claims (4)

1. an indoor artificial cultivation method of codling moth, the growth process of described codling moth is "egg-larvae-pupa-adult", and lays eggs after mating by adults to realize population reproduction, which is characterized in that , the indoor artificial breeding method comprises the following steps: (1) Establishment of codling moth breeding environment Choose a separate breeding room. The breeding room is equipped with windows that can be opened and closed, and a separate sealable breeding room is established in the breeding room. Air conditioners, humidifiers, lighting, ultraviolet lights, temperature sensors, and humidity are installed in the breeding room. Sensors, and set up a breeding platform and racks in the breeding room, on which are placed net bags, corrugated paper, egg paper, centrifuge tubes, absorbent cotton, toothpicks, egg cartons, artificial climate boxes, egg-laying boxes, Purified water, sodium hypochlorite disinfection water, honey water, special feed, petri dishes, and all the above items are sterilized and then placed in the breeding room, and the spawning box is placed in the artificial climate box; (2) Collection of codling moth eggs Put the bored fruits with codling moth larvae collected from the field into mesh bags, and place them in an artificial climate box for rearing, keeping the temperature T (25±1)°C and humidity RH (70±5) in the artificial climate box %, photoperiod L: D = 16: 8h, put some corrugated paper in the mesh bag at the same time, lure the larvae to cocoon and pupate, and collect them into the spawning box after the pupae emerge into adults, and supply honey water with a concentration of 10% , One-sided egg-covered paper in the spawning box to induce adult worms to lay eggs, replace the egg paper every 2-3 days according to the amount of adult eggs laid, and record the date of replacing the egg paper, and then put the replaced egg paper into the built-in moist absorbent cotton Put the egg carton into the artificial climate box and wait for the eggs to hatch. Before the eggs hatch, that is, the blackhead stage of the eggs, disinfect them with sodium hypochlorite water with a concentration of 1‰, rinse them with pure water for 2-3 times, and wait for the eggs to hatch into larvae. ; (3) Indoor breeding of codling moth Feed in sterilized centrifuge tubes, fill each centrifuge tube with special feed, connect the larvae to the centrifuge tube, plug the tube mouth with sterilized absorbent cotton, and put the larvae hatched on the same day into the same centrifuge tube, and Record the date, and then check the centrifuge tube once a week to remove dead insects in time to prevent bacteria and viruses from infecting other healthy larvae in the centrifuge tube. When the mature larvae pupate, collect the pupae and put them in a culture dish with moist absorbent cotton, and culture them The dishes are placed in the spawning box placed in the artificial climate box, and the temperature T (25 ± 1) °C, humidity RH (70 ± 5)%, and photoperiod L: D = 16: 8h are kept in the artificial climate box all the time. Its adults emerge, mate, lay eggs, and collect the eggs of the next generation, so that they are continuously reared to form an indoor codling moth population. 2. the indoor artificial breeding method of a kind of codling moth according to claim 1, it is characterized in that, described adopting from the bore fruit with codling moth larva in the field needs to be sterilized, and disinfection method is to adopt to soak sodium hypochlorite Wipe the surface of the bored fruit with a disposable cloth towel soaked in disinfectant water, and then wipe the surface of the bored fruit again with a disposable cloth towel soaked in purified water. It needs to be wrung out to ensure that the disposable cloth towel is moist and free of dripping liquid, so as to prevent sodium hypochlorite disinfection water and pure water from entering the inside of the bored fruit. 3. the indoor artificial breeding method of a kind of codling moth according to claim 1, is characterized in that, described centrifuge tube volume is 5mL, and the amount of putting into special feed in centrifuge tube is 2mL. 4. The indoor artificial cultivation method of a kind of codling moth according to claim 1, characterized in that, the petri dish is made of special feed for codling moth.