Fatma Aly Ahmed
Ain Shams University, Botany/chemistry, Department Member
- Name: Fatma Aly Ahmed Aly Nationality : Egyptian Current Position : Former Chairman of Ecology and Dry Lands Agricu... moreName: Fatma Aly Ahmed Aly Nationality : Egyptian
Current Position : Former Chairman of Ecology and Dry Lands Agriculture Division, Desert Research Center, Cairo, Egypt from 14 Dec. 2016 till 14 Dec. 2022.
Ex Vice President of DRC for Research, Studies and Projects, Ministry of Agriculture and Land Reclamation (28 Nov. 2013 till 27 Aug 2015).edit
Originally from Mexico, today, prickly pears (Opuntia ficus-indica (L.) Mill.) can be found worldwide, but they are most common in Africa., Australia, and the countries of the Mediterranean. The Cactaceae family, which has more than 1500... more
Originally from Mexico, today, prickly pears (Opuntia ficus-indica (L.) Mill.) can be found worldwide, but they are most common in Africa., Australia, and the countries of the Mediterranean. The Cactaceae family, which has more than 1500 species of cactus, includes prickly pears. Additionally, consumers are becoming more and more interested in it because it contains bioactive substances like carotenoids, sterols, polyunsaturated fatty acids, polyphenols, and vitamins that are linked to important biological and functional activities such as effects on antioxidants, inflammation, and hypoglycemia as it prospers in dry and semiarid areas, this plant is very effective in preventing desertification and can be exploited in fields and gardens as a fence. Although it is frequently used today to make culinary products like jams and juices, it is also eaten as a fruit. If one wishes to create novel products with health-promoting properties for food, cosmetics, or pharmaceutical fields, as a feasible wellspring of phytochemicals for practical food sources, natural additions, or nutritional supplements, prickly pears are affordable.
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This study was conducted to investigate the ability of hesperidin (HES) to form metal-complexes (HES-M) by chelation with transition metals such iron(III), copper(II), zinc(II), and silver(I), in addition to screen the antioxidant... more
This study was conducted to investigate the ability of hesperidin (HES) to form metal-complexes (HES-M) by chelation with transition metals such iron(III), copper(II), zinc(II), and silver(I), in addition to screen the antioxidant activity (DPPH free radical scavenging and phosphomolybdenum reduction potential methods PRP),
anti-Alzheimer activity [(acetylcholinesterase (AChE)] inhibition assay) and coagulant activity (prothrombin time PT assay). The results indicated that HES could form a 1:1 complex with the studied metals in methanolic solution. The complex has been synthesized and characterized by physicochemical methods. Complexing of hesperidin with metals leads to increase in the antioxidant activity by 15.4-33%. Also, the total antioxidant capacities of HES-M complexes were increased by 13.8-251%. Cu-complex revealed the most increasing antioxidant activity by 33% for
DPPH scavenging activity and 251% for PRP activity. The inhibition capacity of the HES, as well as of the HESM complexes on the enzyme AChE, was revealed that only HES-Zn complex has higher anti-Alzheimer activity (IC50 1.18 mg/mL). HES and HES-M complexes led to accelerate the clotting time by 28.9-67.3%, where HES-Zn complex was the most accelerating clotting time by 67.3%. The high increase in total antioxidant activity of hesperidin after chelating with copper (251%) opens the door for further research.
anti-Alzheimer activity [(acetylcholinesterase (AChE)] inhibition assay) and coagulant activity (prothrombin time PT assay). The results indicated that HES could form a 1:1 complex with the studied metals in methanolic solution. The complex has been synthesized and characterized by physicochemical methods. Complexing of hesperidin with metals leads to increase in the antioxidant activity by 15.4-33%. Also, the total antioxidant capacities of HES-M complexes were increased by 13.8-251%. Cu-complex revealed the most increasing antioxidant activity by 33% for
DPPH scavenging activity and 251% for PRP activity. The inhibition capacity of the HES, as well as of the HESM complexes on the enzyme AChE, was revealed that only HES-Zn complex has higher anti-Alzheimer activity (IC50 1.18 mg/mL). HES and HES-M complexes led to accelerate the clotting time by 28.9-67.3%, where HES-Zn complex was the most accelerating clotting time by 67.3%. The high increase in total antioxidant activity of hesperidin after chelating with copper (251%) opens the door for further research.
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The history for the use of Aloe vera for medicinal purposes starts from about 3000 years. Medicinally, this plant may be able to treat skin cancer as well as sunburns, burns, and small wounds. More than 104 compounds from various parts of... more
The history for the use of Aloe vera for medicinal purposes starts from about 3000 years. Medicinally, this plant may be able to treat skin cancer as well as sunburns, burns, and small wounds. More than 104 compounds from various parts of this plant, including minerals, vitamins, amino acids, enzymes, sterols, anthraquinone, flavonoids, terpenoids, coumarins, polysaccharides, sugars, and polyphenols, have been isolated thus far, according to scientific reports on phytochemical analysis of this plant. The biological activities of these compounds are diverse and include anthelmintic, hepatoprotective, antidiabetic, diuretic, antibacterial, antiviral, antioxidant, antiseptic, anti-inflammatory, anticancer, and cosmetic effects for medical therapy. This article mainly emphasizes therapeutic, medicinal and food uses of A. vera.
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n this study, GC/MS of volatile oils and lipid constituents were explored for four Apiaceae family members: Deverra tortuosa (Desf.) DC., Daucus syrticus L., Anethum graveolens L. and Petroselinum crispum (Mill.). The potential of using... more
n this study, GC/MS of volatile oils and lipid constituents were explored for four Apiaceae family members: Deverra tortuosa (Desf.) DC., Daucus syrticus L., Anethum graveolens L. and Petroselinum crispum (Mill.). The potential of using the ethanolic extracts of these plants in the synthesis of green silver nanoparticles (AgNPs) was investigated. Additionally, the effect of these synthesized nanoparticles on some strains of bacteria and fungi was studied. About the lipoidal matter, the data show that, D. tortuosa and D. syrticus had 32 and 13 compounds, respectively, while A. graveolens and P. crispum had 24 ,19 compounds, respectively. The Analysis of volatile oils by GC/MS allowed the identification of 31 compounds in D. tortuosa and D. syrticus had 23 compounds, while A. graveolens had 18 compounds and P. crispum had 11 compounds. The data showed that, the size of AgNPs differed according to the plant used in their synthesis as follows: 34.0, 42.7, 37.3, and 41.0 nm for D. tortuosa, D. syrticus, A. graveolens and P. crispum, respectively. It was observed that, AgNPs from P. crispum had the highest effect against Bacillus subtilis (22 mm), while the best activity against Escherichia coli was 20 mm from AgNPs of D. tortuosa ethanolic extract. AgNPs of A. graveolens ethanolic extract had the best effect against Aspergillus versicolor with inhibition zone of 40 mm, followed by D. syrticus (38 mm).
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Salvia officinalis is one of the most important medicinal and aromatic plants in terms of nutritional and medicinal value because it contains a variety of vital active ingredients. Terpenoid compounds, particularly monoterpenes (C10) and... more
Salvia officinalis is one of the most important medicinal and aromatic plants in terms of nutritional and medicinal value because it contains a variety of vital active ingredients. Terpenoid compounds, particularly monoterpenes (C10) and sesquiterpenes, are the most important and abundant among these active substances (C15). Terpenes play a variety of roles and have beneficial biological properties in plants. With these considerations, the current study sought to clone theNAD+-dependent farnesol dehydrogenase (SoFLDH, EC: 1.1.1.354) gene from S. officinalis. Functional analysis revealed that, SoFLDH has an open reading frame of 2,580 base pairs that encodes 860 amino acids.SoFLDH has two conserved domains and four types of highly conserved motifs: YxxxK, RXR, RR (X8) W, TGxxGhaG. However, SoFLDH was cloned from Salvia officinalis leaves and functionally overexpressed in Arabidopsis thaliana to investigate its role in sesquiterpenoid synthases. In comparison to the transgenic plants,...
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Euphorbia dendroides can act as a free radical scavenger, so it protects against acute hepatic damage induced by CCl 4 in rats. Also, it increases the Tm of salmon testis DNA from 68°C to about 81°C. In addition to that, it has shown good... more
Euphorbia dendroides can act as a free radical scavenger, so it protects against acute hepatic damage induced by CCl 4 in rats. Also, it increases the Tm of salmon testis DNA from 68°C to about 81°C. In addition to that, it has shown good antibacterial effect against S. aureus, E. coli, B. subtilis and antifungal effect against A. niger. Along with showing good anticancer effects against HepG2 cells with IC 50 = 9.5 mg/mL, HCT-116 cells with IC 50 = 13.6 mg/mL and MCF-7 with IC 50 = 20.9 mg/mL. The docking study was carried out to explore how genistein, the main compound isolated from the extract of E. dendroides, binds to both VEGFR-2 and DNA active sites. Additionally, our compound displayed very well in Silico predicted ADMET profile. Genistein has better absorption (92.893) compared to sorafenib (84.731) and doxorubicin (62.37), as well as not showing any hepatotoxic effects.
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The authors have requested that this preprint be removed from Research Square.
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Euphorbia dendroides can act as a free radical scavenger, so it protects against acute hepatic damage induced by CCl 4 in rats. Also, it increases the Tm of salmon testis DNA from 68°C to about 81°C. In addition to that, it has shown good... more
Euphorbia dendroides can act as a free radical scavenger, so it protects against acute hepatic damage induced by CCl 4 in rats. Also, it increases the Tm of salmon testis DNA from 68°C to about 81°C. In addition to that, it has shown good antibacterial effect against S. aureus, E. coli, B. subtilis and antifungal effect against A. niger. Along with showing good anticancer effects against HepG2 cells with IC 50 = 9.5 mg/mL, HCT-116 cells with IC 50 = 13.6 mg/mL and MCF-7 with IC 50 = 20.9 mg/mL. The docking study was carried out to explore how genistein, the main compound isolated from the extract of E. dendroides, binds to both VEGFR-2 and DNA active sites. Additionally, our compound displayed very well in Silico predicted ADMET profile. Genistein has better absorption (92.893) compared to sorafenib (84.731) and doxorubicin (62.37), as well as not showing any hepatotoxic effects.
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IAJPS 2015, Volume 2 (5): 961- 966. Abstract: In this study, the primary phytochemical and biological screening of the aerial parts of Trichodesma ehrenbergii Schweinf. ex Boiss. (Family; Boraginaceae)has been done. Phytochemical analysis... more
IAJPS 2015, Volume 2 (5): 961- 966. Abstract: In this study, the primary phytochemical and biological screening of the aerial parts of Trichodesma ehrenbergii Schweinf. ex Boiss. (Family; Boraginaceae)has been done. Phytochemical analysis showed the presence of phenols, flavonoids, alkaloids, terpenoids, steroids, tannins, saponins, and sugars in different extracts of the aerial parts of T. ehrenbergii .The concentration of phenols, flavonoids, alkaloids and tannins and saponins were found in the range 138±0.8mg/g (as gallic acid equivalent), 123±0.6mg/g (as rutin equivalent), 1.3±0.6mg/g, 2.7±0.2mg/g and 6.7±0.2mg/g, respectively. Combined and free amino acids analysis showed the presence of 17 amino acids as free and protein amino acids. Glutamic acid (29.919ppm) and lysine (72.640ppm) represented as the major components of free and protein amino acids, respectively. The antimicrobial activity of methanol extract of the aerial parts of T. ehrenbergii against 6 bacterial strainsand 5 fungal strains by a pour plate technique method was determined. Keywords: Trichodesma ehrenbergii, Gebel Elba, Phytochemical screening, Primary and Secondary metabolites, Eco-physiological analysis, Amino acids, Antimicrobial Activity.
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This study was carried out on a cactus pear orchard grown in calcareous soil at El-Hammam region, Matrouh Governorate, over two consecutive seasons in 2020 and 2021. This work aimed to find the best mineral fertilization treatment for... more
This study was carried out on a cactus pear orchard grown in calcareous soil at El-Hammam region, Matrouh Governorate, over two consecutive seasons in 2020 and 2021. This work aimed to find the best mineral fertilization treatment for increasing the yield of plants, as it has yet to be studied under the conditions of this region. The study was a randomized complete block experiment. The fertilization treatments were as the following:-(control, 100 g N + 40 g P + 70 g K/plant, 100 g N + 40 g P + 90 g K/plant, 100 g N + 60 g P + 70 g K/plant, 100 g N + 60 g P + 90 g K/plant, 120 g N + 40 g P + 70 g K/plant, 120 g N + 40 g P + 90 g K/plant, 120 g N + 60 g P + 70 g K/plant and 120 g N + 60 g P + 90 g K/plant). Increasing nitrogen, phosphorus, and potassium fertilization rates improved vegetative growth, fruit quantity, and quality traits over control. The highest fertilization rate (120 g N + 60 g P + 90 g K/plant) significantly recorded the best physical characteristics (fruit weight, fruit length, fruit width, fruit volume, juice weight, peel weight, peel thickness, pulp weight/fruit, number of seeds/fruit, and seeds weight/fruit). Furthermore, fruits had suitable chemical parameters (total soluble solids, total acidity content, total soluble solids/acid ratio, and ascorbic acid content). The most useful chemical fertilization amount was the basal dressing of 120 g N, 60 g P, and 90 g K/plant in this area.
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The chemical investigation of the chloroform and ethyl acetate extracts of the aerial parts of Forsskaolea viridis Ehrenb. ex Webb (Family: Urticaceae) led to isolation eight compounds for the first time, from the plant where two phenolic... more
The chemical investigation of the chloroform and ethyl acetate extracts of the aerial parts of
Forsskaolea viridis Ehrenb. ex Webb (Family: Urticaceae) led to isolation eight compounds for the first time,
from the plant where two phenolic compounds, identified as ρ-coumaric and caffeic acids as well as six
flavonoid compounds identified as 5-hydroxy-6,7,3í,4í-tetramethoxy flavone, chrysoeriol, acacetin, chrysoeriol-
7-O-fl-D-glucoside, kaempferol-3-O-(2î-O-Eóp-coumaroyl)-fl-D-glucoside and isovetixin. The chemical
structure of the isolated compounds was established by spectroscopic methods including UV, MS, 1H-NMR,
and 13C-NMR. Antimicrobial, antioxidant and cytotoxic activities of the ethyl acetate and chloroform extracts
were evaluated. The ethyl acetate extract exhibited strong antimicrobial activity (12-30 mm) against the tested
strains. The ethyl acetate and chloroform extracts showed fair antioxidant and cytotoxicity.
Forsskaolea viridis Ehrenb. ex Webb (Family: Urticaceae) led to isolation eight compounds for the first time,
from the plant where two phenolic compounds, identified as ρ-coumaric and caffeic acids as well as six
flavonoid compounds identified as 5-hydroxy-6,7,3í,4í-tetramethoxy flavone, chrysoeriol, acacetin, chrysoeriol-
7-O-fl-D-glucoside, kaempferol-3-O-(2î-O-Eóp-coumaroyl)-fl-D-glucoside and isovetixin. The chemical
structure of the isolated compounds was established by spectroscopic methods including UV, MS, 1H-NMR,
and 13C-NMR. Antimicrobial, antioxidant and cytotoxic activities of the ethyl acetate and chloroform extracts
were evaluated. The ethyl acetate extract exhibited strong antimicrobial activity (12-30 mm) against the tested
strains. The ethyl acetate and chloroform extracts showed fair antioxidant and cytotoxicity.
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This study was carried out on a cactus pear orchard grown in calcareous soil at El-Hammam region, Matrouh Governorate, over two consecutive seasons in 2020 and 2021. This work aimed to find the best mineral fertilization treatment for... more
This study was carried out on a cactus pear orchard grown in calcareous soil at El-Hammam region, Matrouh Governorate, over two consecutive seasons in 2020 and 2021. This work aimed to find the best mineral fertilization treatment for increasing the yield of plants, as it has yet to be studied under the conditions of this region. The study was a randomized complete block experiment. The fertilization treatments were as the following:-(control, 100 g N + 40 g P + 70 g K/plant, 100 g N + 40 g P + 90 g K/plant, 100 g N + 60 g P + 70 g K/plant, 100 g N + 60 g P + 90 g K/plant, 120 g N + 40 g P + 70 g K/plant, 120 g N + 40 g P + 90 g K/plant, 120 g N + 60 g P + 70 g K/plant and 120 g N + 60 g P + 90 g K/plant). Increasing nitrogen, phosphorus, and potassium fertilization rates improved vegetative growth, fruit quantity, and quality traits over control. The highest fertilization rate (120 g N + 60 g P + 90 g K/plant) significantly recorded the best physical characteristics (fruit weight, fruit length, fruit width, fruit volume, juice weight, peel weight, peel thickness, pulp weight/fruit, number of seeds/fruit, and seeds weight/fruit). Furthermore, fruits had suitable chemical parameters (total soluble solids, total acidity content, total soluble solids/acid ratio, and ascorbic acid content). The most useful chemical fertilization amount was the basal dressing of 120 g N, 60 g P, and 90 g K/plant in this area.
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The purpose of the present study was to assess fresh juice and peels of cactus pear Opuntia ficus-indica (yellow cultivar) and Opuntia littoralis (red cultivar),local origin for their Physicochemical properties and bioactive compounds as... more
The purpose of the present study was to assess fresh juice and peels of cactus pear Opuntia ficus-indica (yellow cultivar) and Opuntia littoralis (red cultivar),local origin for their Physicochemical properties and bioactive compounds as well as jelly candies prepared by using six formulas of prickly pear juice and peels were studied .The obtained results showed that, prickly pear juices (red and yellow cultivars) were the highest T.S.S (12.3 and 11.9%, respectively) and PH values (5.2 and 5.7, respectively), while the lowest values were recorded for the peels. On the contrary, the peels possessed the highest total phenolic contents (87.72 and 76.15 mg Gallic acid/100 g FW) and flavonoids (18.182 and 13.55 mg Querectin/100 g FW) for red and yellow, respectively. Whereas, red prickly pear showed lower content of carotenoids and higher content of betalains than yellow prickly pear (either for juice or peels). On the other hand, results showed that jelly candies produced from prickly pear are found to be important source of total polyphenols, flavonoids, carotenoids, and betalains and have low microbial load during cold storage for 4weeks. Sensorial evaluation showed that red peels, red juice and yellow juice jelly candies were the most accepted by panelists with the same values which considered "Like very Much " .Therefore, prickly pear fruits can be used for food manufacturing especially children's foods which are considered to be healthy, attractive colors, have no side effects and are appropriate in sensorial properties which leads to an increase in the add value.
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Salvia officinalis is one of the most important medicinal and aromatic plants in terms of nutritional and medicinal value because it contains a variety of vital active ingredients. Terpenoid compounds, particularly monoterpenes (C10) and... more
Salvia officinalis is one of the most important medicinal and aromatic plants in terms of nutritional and medicinal value because it contains a variety of vital active ingredients. Terpenoid compounds, particularly monoterpenes (C10) and sesquiterpenes, are the most important and abundant among these active substances (C15). Terpenes play a variety of roles and have beneficial biological properties in plants. With these considerations, the current study sought to clone theNAD+-dependent farnesol dehydrogenase (SoFLDH, EC: 1.1.1.354) gene from S. officinalis. Functional analysis revealed that, SoFLDH has an open reading frame of 2,580 base pairs that encodes 860 amino acids.SoFLDH has two conserved domains and four types of highly conserved motifs: YxxxK, RXR, RR (X8) W, TGxxGhaG. However, SoFLDH was cloned from Salvia officinalis leaves and functionally overexpressed in Arabidopsis thaliana to investigate its role in sesquiterpenoid synthases. In comparison to the transgenic plants, the wild-type plants showed a slight delay in growth and flowering formation. To this end, a gas chromatography-mass spectrometry analysis revealed that SoFLDH transgenic plants were responsible for numerous forms of terpene synthesis, particularly sesquiterpene. These results provide a base for further investigation on SoFLDH gene role and elucidating the regulatory mechanisms for sesquiterpene synthesis in S. offcinalis. And our study paves the way for the future metabolic engineering of the biosynthesis of useful terpene compounds in S. offcinalis.
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Cactus pear pulp and peel are rich sources of important nutrients and bioactive compounds, which are degraded during dehydration. This work aims to determine the chemical characteristics of fresh juice and peel of Opuntia ficus-indica... more
Cactus pear pulp and peel are rich sources of important nutrients and bioactive compounds, which are degraded during dehydration. This work aims to determine the chemical characteristics of fresh juice and peel of Opuntia ficus-indica (yellow cultivar) and Opuntia littoralis (red cultivar), using them in cookies production and evaluating the quality of cookies. Results showed higher TSS, polyphenols, flavonoids and betalains for the red cultivar (either juice or peels), while the yellow cultivar possessed higher pH and carotenoids. The major phenolic acids of all samples are gallic followed by chlorogenic. While the predominant flavonoids are catechins followed by naringenin. Yellow and red juice cookies possessed higher moisture. Yellow or red peel cookies owned higher crude fiber. Yellow and red peel cookies recorded the best microbiological quality. Peel cookies had higher weight and hardness compared to juice cookies which had higher diameters and spread ratio. All produced cookies, are organoleptically accepted. The results suggested that, fresh prickly pear juices and peels could be used in the production of delicious and healthy cookies.
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Neotorularia aculeolata belongs to the family Cruciferae that has several uses in the Egyptian folk medicine for many years. Nothing could be traced about the chemical composition of the plant. Extraction, isolation and purification of... more
Neotorularia aculeolata belongs to the family Cruciferae that has several uses in the Egyptian folk medicine for many years. Nothing could be traced about the chemical composition of the plant. Extraction, isolation and purification of the air-dried plant material using different chromatographic techniques (PC, TLC & CC) provided seven flavonoids. Identification of the isolated compounds using different chemical and physical techniques (UV, 1H-NMR and C 13 NMR spectroscopy) allowed to characterize these compounds as kaempferol, kaempferol-7-O-rhamnoglucoside {Kaempferol-7-neohesperidoside}, quercetin, rutin, quercetin-3-O- β-D-glucoside-7-O-α-L-rhamnoside-3`-methylether, quercetin-3,7-di-O-α-L-rhamnoside-3`-methylether and myricetin.
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Aerial parts of Varthemia candicans were collected seasonally for one year (winter, spring, summer and autumn) from Wadi Habbes (rocky habitat) and Sand Dunes habitat, West Marsa Matrouh, Egypt. The results of the plant photochemical... more
Aerial parts of Varthemia candicans were collected seasonally for one year (winter, spring, summer and autumn) from Wadi Habbes (rocky habitat) and Sand Dunes habitat, West Marsa Matrouh, Egypt. The results of the plant photochemical analysis cleared out that the amount of soluble carbohydrates in the study habitats during the wet seasons (winter and autumn) was higher than that of the dry season, however the content of insoluble carbohydrates recorded a reverse trend. HPLC analysis of free sugars detected the occurrence of glucoronic acid, raffinose, glucose, galactose, fructose and fucose. The most abundant free sugar in WH was glucoronic acid but in SD was raffinose. Also, HPLC analysis of combined sugars detected the presence of glucose, mannose, fructose and maltose with the commonness of maltose in the two study habitats. The amount of soluble amino acids and soluble proteins were greater in SD habitat than WH habitat during all seasons, except autumn season. Free amino acids ...
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Two Phlomis species occur in Egypt: P. aurea (Decne.), endemic to Sinai region and P. floccosa (Don.) in the western Mediterranean coast. The carbohydrate composition of plants varied according to the species, microhabitat, and organ.... more
Two Phlomis species occur in Egypt: P. aurea (Decne.), endemic to Sinai region and P. floccosa (Don.) in the western Mediterranean coast. The carbohydrate composition of plants varied according to the species, microhabitat, and organ. Soluble sugars (SS) were in the order: leaves > flowers > stem in the two species, with higher levels in P. aurea than P. floccosa. By contrast, insoluble sugars (IS), being comparable in the two species, were in the order stem > flowers > leaves. Uronic acids constituted the major sugar fraction in the two species, with higher levels in P. aurea than P. floccosa and different organ allocation in the two species. By contrast, monosaccharide and disaccharide fractionations varied in the two species. Whereas arabinose and xylose were the predominant monosaccharides in SS of P. aurea, fructose and mannose predominated in P. floccosa. Likewise, whereas IS of P. aurea lacks galactose, with high levels of xylose and arabinose the reverse was true for P. floccosa. Disaccharides within SS of P. aurea were highest in flowers and least in leaves with the predominance of sucrose over maltose, but the reverse was true in P. floccosa. As for IS, sucrose surpassed maltose in both species. Stachyose was the only oligosaccharide detected, with contrasting distribution in the two species. Sugar alcohols in SS were higher in the foliage of the two species than the flowers with the predominance of mannitol over sorbitol, but in the case of IS, only mannitol exists, with contrasting organ allocation in the two species.
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The present research was carried out for two main targets, the first one is phytochemical and biological screening of the aerial parts of Silybum marianum. The second one is the possibility of using Silybum marianum ethanolic extract as... more
The present research was carried out for two main targets, the first one is phytochemical and biological screening of the aerial parts of Silybum marianum. The second one is the possibility of using Silybum marianum ethanolic extract as natural preservative for extending minced beef shelf-life during refrigeration and frozen storage. The chemical analysis of dry matter revealed that total ash content was 13.04% of D.M, crude fibers, total phenolic compounds, total flavonoids and total tannins were (24.59%, 9.16%, 0.84% and (0.08%), respectively. The highest values of antioxidant activity were recorded in ethyl acetate and ethyl alcohol 70%extacts, respectively for successive and selective solvents with values of 82.45% and 58.85% for successive solvents, 86.84% and 70.50% for selective solvents, respectively. Antimicrobial activity showed that, S. marianum L. Gaertn. had effect on gram positive, gram negative bacteria and yeast but had no effect on different strains of studied fungi. S. marianum had an influence on microorganisms growth in minced beef, the highest effect was 1.0% for 60 days (56 CFU). The most effective concentrations on lipid peroxidation prevention in minced beef were 1.5% for 3 days and 1.5 for 60 days.
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The present research was carried out for two main targets, the first one is phytochemical and biological screening of the aerial parts of Silybum marianum. The second one is the possibility of using Silybum marianum ethanolic extract as... more
The present research was carried out for two main targets, the first one is phytochemical and biological screening of the aerial parts of Silybum marianum. The second one is the possibility of using Silybum marianum ethanolic extract as natural preservative for extending minced beef shelf-life during refrigeration and frozen storage. The chemical analysis of dry matter revealed that total ash content was 13.04% of D.M, crude fibers, total phenolic compounds, total flavonoids and total tannins were (24.59%, 9.16%, 0.84% and (0.08%), respectively. The highest values of antioxidant activity were recorded in ethyl acetate and ethyl alcohol 70%extacts, respectively for successive and selective solvents with values of 82.45% and 58.85% for successive solvents, 86.84% and 70.50% for selective solvents, respectively. Antimicrobial activity showed that, S. marianum L. Gaertn. had effect on gram positive, gram negative bacteria and yeast but had no effect on different strains of studied fungi. S. marianum had an influence on microorganisms growth in minced beef, the highest effect was 1.0% for 60 days (56 CFU). The most effective concentrations on lipid peroxidation prevention in minced beef were 1.5% for 3 days and 1.5 for 60 days.
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Phlomis aurea Decne. and Phlomis floccosa D. Don. exhibit different ecological and geographical distribution range in Egypt. Phlomis aurea is endemic to Saint Katherine Protectorate (SKP), South Sinai with narrow geographic distribution,... more
Phlomis aurea Decne. and Phlomis floccosa D. Don. exhibit different ecological and geographical distribution range in Egypt. Phlomis aurea is endemic to Saint Katherine Protectorate (SKP), South Sinai with narrow geographic distribution, but wide ecological range. Phlomis floccosa has wide geographical and ecological range within the coastal western Mediterranean coast of Egypt. Unlike P. floccosa, P. aurea has wide altitudinal range which increases its resistance to climate changes. Here, we assessed the effect of microhabitats on growth parameters and plant vigor of P. aurea and P. floccosa. The maximum EC of the soil in the various microhabitats supporting P. floccosa (950 µS/cm) is substantially higher than that of P. aurea (110 µS/cm). Both Phlomis species had low number of associated species which may be related to habitat aridity. The results indicate that the microhabitats supporting both species are the best environments for future conservation of these species under the impact of climate change.
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Phlomis aurea Decne. and Phlomis floccosa D. Don. exhibit different ecological and geographical distribution range in Egypt. Phlomis aurea is endemic to Saint Katherine Protectorate (SKP), South Sinai with narrow geographic distribution,... more
Phlomis aurea Decne. and Phlomis floccosa D. Don. exhibit different ecological and geographical distribution range in Egypt. Phlomis aurea is endemic to Saint Katherine Protectorate (SKP), South Sinai with narrow geographic distribution, but wide ecological range. Phlomis floccosa has wide geographical and ecological range within the coastal western Mediterranean coast of Egypt. Unlike P. floccosa, P. aurea has wide altitudinal range which increases its resistance to climate changes. Here, we assessed the effect of microhabitats on growth parameters and plant vigor of P. aurea and P. floccosa. The maximum EC of the soil in the various microhabitats supporting P. floccosa (950 µS/cm) is substantially higher than that of P. aurea (110 µS/cm). Both Phlomis species had low number of associated species which may be related to habitat aridity. The results indicate that the microhabitats supporting both species are the best environments for future conservation of these species under the impact of climate change.
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Present study focused on evaluation of the effect of national nancomposite product on serum biochemical profile and programmed cell death (apoptosis) by using Annexin V-FITC and PI test of breast carcinoma female rats. Basic nano-curcumin... more
Present study focused on evaluation of the effect of national nancomposite product on serum biochemical profile and programmed cell death (apoptosis) by using Annexin V-FITC and PI test of breast carcinoma female rats. Basic nano-curcumin was tested for its protective effect on breast cancer female rats (n= 30) divided in two main groups (NTBR) and (TBR) which in turn classified into (TBR-Control Positive and TBR-basic nano-curcumin protective groups). Basic nano-curcumin was formulated and tested for both IC50 (IC50 = 1.0 µg) and LD5o (35 mg/kg by wt.) by using in vitro and in vivo techniques, respectively. Breast cells apoptosis was determined by Annexin V-FITC, which is a fluorescent probe binds to phosphotidylserine (PS) in a Ca 2+ dependent pathway. Breast Cells were stained with Annexin V and PI. The apoptotic cells [Annexin (+) and PI (-)] were differentiated from necrotic cells [Annexin (-) and PI (+)] using flow cytometry technique. The anti-proliferative effect of basic nano-curcumin appeared much earlier due to its nano form. Also, Liver [ALT (40U/l), AST (85 U/l)] and kidney [BUN (19 mg/dl), Creatinine (0.5 mg/dl)] functions were decreased within normal ranges. keyword: Basic nano-curcumin, Annexin, BUN, Creatinine, AST, ALT.
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Atriplex canescens (fourwing saltbush), is an attractive plant for erosion control and reclamation of marginal lands due to its excellent adaptability. A greenhouse pot experiment was carried out at the greenhouse of Environment and... more
Atriplex canescens (fourwing saltbush), is an attractive plant for erosion control and reclamation of marginal lands due to its excellent adaptability. A greenhouse pot experiment was carried out at the greenhouse of Environment and Bio-agriculture Department, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt during 2015/2016 seasons, to study the effect of NaCl concentrations in irrigation water on growth, cations, anions and antioxidant enzymes activities of A. canescens. Three-months-old, uniform sized seedlings of A. canescens were irrigated with solution containing 0, 150, 300, 450 and 600 mM NaCl for 3 months. The results showed that, the addition of 150mM NaCl significantly increased fresh weight of A. canescens plants compared to control plants. Furthermore, the addition of both 300,450 and 600mM NaCl significantly reduced fresh weight of plant, compared with the control. The higher concentrations of NaCl in irrigation water reduced the dry weight. Additionally, the calcium (Ca2+), magnesium (Mg2+), and phosphorus (P3+), concentrations were decreased with increasing NaCl level. However, sodium (Na+) and chloride (Cl−) concentrations, and antioxidant enzymes activities were increased by increasing NaCl concentrations in irrigation water.
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Objective: The objective of this study is to isolate and evaluate the antioxidant, antimicrobial, and antitumor activity of the methanolic constituents of Forsskaolea viridis. Methods: The isolation and purification of phytochemical... more
Objective: The objective of this study is to isolate and evaluate the antioxidant, antimicrobial, and antitumor activity of the methanolic constituents of Forsskaolea viridis. Methods: The isolation and purification of phytochemical constituents were performed in the present study using various chromatographic methods (thin-layer [TLC], preparative paper [PPC], and column chromatography [CC]) where, the structures of isolated compounds were determined using different spectroscopic methods (Mass spectroscopy [Ms], ultraviolet [UV], proton and carbon nuclear magnetic resonance [ 1 H-NMR and 13 C-NMR]) and confirmed by data reported in the literature. The biological activity of the methanolic extract was performed as in vitro studies by diffusion agar technique, 2,2-Diphenyl-1-picrylhydrazyl hydrate (DPPH), and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay for antimicrobial, antioxidant, and antitumor activity, respectively. Results: The chemical studies led to the isolation of five flavonoids and two phenolic compounds for the first time from this plant. The methanolic extract exhibited potent antimicrobial activity (13-26 mm) against some of the tested strains while it showed fair antioxidant and cytotoxicity activity. Conclusion: It can be concluded from the results that the methanolic extract of F. viridis has potential antimicrobial activity while fair antioxidant and antitumor activity.
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S OME nutritional vitamin, flavonoid and phenolic compounds of Forsskaolea viridis aerial parts were extracted and investigated for the first time by using high performance liquid chromatography (HPLC). A comparative analysis of... more
S OME nutritional vitamin, flavonoid and phenolic compounds of Forsskaolea viridis aerial parts were extracted and investigated for the first time by using high performance liquid chromatography (HPLC). A comparative analysis of fat-soluble vitamins (A, D, E and K) and water-soluble vitamins (C and B complex) were carried out. Results of this study revealed that, the presence of vitamin A (retinol) as the highest concentrated fat soluble vitamins (332.591μg/100g), and the presence of water-soluble vitamins, in which vitamin C (ascorbic acid) represented relatively high concentration (234.21μg/100g), followed by vitamin B12 (cobalamin) (386.06 μg/100g) and vitamin B2 (riboflavin) (112.16 μg/100g) in the aerial parts of Forsskaolea viridis. The outlined results of HPLC investigation of methanolic extract showed the presence of 18 phenolic and 26 flavonoid known compounds, which were identified for the first time from the aerial parts of Forsskaolea viridis. The high concentration of vitamins, flavonoid compounds and phenolic acids contents in the plant reflected to the medicinal importance of the plant as anticancer, antioxidant and antimicrobial activity which play an important role in human health and plant growth.
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The aim of this study was to evaluate the antibacterial activity of eight essential oils against some multi-drug resistant Gram negative bacteria (three different isolates of each Acinetobacter baumannii and Klebsiella pneumoniae). The... more
The aim of this study was to evaluate the antibacterial activity of eight essential oils against some multi-drug resistant Gram negative bacteria (three different isolates of each Acinetobacter baumannii and Klebsiella pneumoniae). The hydrodistilled essential oils of the fresh aerial part of some medicinal plants belonging to family Lamiaceae namely: Origanum majorana L. , Origanum majorana L. , Origanum syriacum L., Thymus capitatus L., Thymus vulgaris L., Salvia fruticosa Mill., Mentha virdis L. and Lavandula officinalis L. were subjected to Gas Chromatography−Mass spectrometry analysis. This revealed 25, 22, 22, 23, 23, 27, 19 and 31 compounds had been identified in the essential oils, respectively. The major components were identified as terpinen-4-ol (21.99%), terpinen-4-ol (23.56%), cymene (27.98%), carvacrol (41.55%), m-thymol (23.97%), eucalyptol (54.84%), carvone (47.79%) and eucalyptol (36.42%), respectively. Antibacterial activity was carried out and the activities were correlated to chemical composition of analyzed essential oils. We found five essential oils were the most effective. The study concluded that, some essential oils of Egyptian medicinal plants belonging to family Lamiaceae can be used to treat infections caused by multi-drug resistant gram negative bacteria.
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Periploca angustifolia L is a wild plant is grown at the northwestern coast of Egypt. Traditional uses of this species drive us to evaluate the phytochemical and pharmaco-toxicological aspects of the ethanolic extract of the plant.... more
Periploca angustifolia L is a wild plant is grown at the northwestern coast of Egypt. Traditional uses of this species drive us to evaluate the phytochemical and pharmaco-toxicological aspects of the ethanolic extract of the plant. Phytochemical screening revealed the presence of various bioactive secondary metabolites as flavonoids, phenolics, coumarins, terpenes, tannins, and sterols compounds which might be responsible for their medicinal attributes. Alkaloids, cardiac glycosides and volatile oils were not detected. The safety of ethanolic extract of Periploca angustifolia L is evidenced by the high LD50 value of the extract (>5g/kg). In addition, there wasn't significant modification in the general behavior of the animals and deaths after 72 hours post-administration. The extract (500 mg kg −1) orally induced a diuretic effect in rats by increasing urine output. Diuretic activity of was assessed in rats with furosemide as a standard drug using Lipschitzs test. Diuretic potency of ethanolic extract of Periploca angustifolia L was found to be 77% of furosemide as a standard drug using Lipschitzs test. Administration of ethanolic extract increased the renal excretion of Na + , K + levels without altering both ratio of Na + / K + and Cl-/ Na + + K + in serum and urine of treated rats. In addition, there was no alkalization of urine and without alteration in Na + /K + ratio i.e. aldosterone secretion index. The present findings sustained the traditional use of P. angustifolia as a diuretic.
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Background: In traditional North Africa, medicine decoctions of the leaves of Periploca angustifolia are used to treat diarrhea, inflammation, ulcers, edema and diabetes. The aim of the study was to evaluate the phytochemical,... more
Background: In traditional North Africa, medicine decoctions of the leaves of Periploca angustifolia are used to treat diarrhea, inflammation, ulcers, edema and diabetes. The aim of the study was to evaluate the phytochemical, anti-inflammatory, anti-ulcerogenic, and hypoglycemic activities of an ethanolic extract of P. angustifolia L. in rats. Methods: An extract of air-dried powdered P. angustifolia plant was obtained using 96% ethanol. The extract was concentrated and the total phenolic and flavonoids contents were estimated colorimetrically. The phenolic and flavonoid compounds were quantified and identified using high performance liquid chromatography (HPLC). The anti-inflammatory, anti-ulcerogenic and hypoglycemic activities of the extract were evaluated in three rat models respectively: formaldehyde-induced paw edema, ethanol induced gastric damage and alloxan induced hyperglycemia. Results: The total flavonoids and total phenolics constituted 15% and 2.69% of the extract, respectively and are expressed as quercetin equivalent and μg/mg gallic acid equivalent (GAE). Coumarin, resorcinol, isorhamnetin, quercetin, and naphthalene were isolated from the ethanolic extract of P. angustifolia. Oral administration of the ethanolic extract at 500 mg/kg body weight (b.wt.) significantly reduced paw inflammation, gastric lesions, ulcer index scores and blood glucose levels in normal and diabetic rats.
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In this study, the proximate analysis and primary phytochemical screening of the aerial parts of Forsskaolea viridis Ehrenb. ex Webb (Family: Urticaceae) has been done. Proximate analysis showed that the percentage of total ash was (16.77... more
In this study, the proximate analysis and primary phytochemical screening of the aerial parts of Forsskaolea viridis Ehrenb. ex Webb (Family: Urticaceae) has been done. Proximate analysis showed that the percentage of total ash was (16.77 ± 0.22), crude fibers (10.82 ± 0.41), total lipids (6.27 ± 0.07) and the chemical and physical properties of lipids were determined, total protein was (8.81± 0.45), total carbohydrates was (34.72± 0.41) and nutritive value of the plant determined. Phytochemical screening showed the presence of phenols, flavonoids, alkaloids, terpenoids, steroids, tannins, saponins, and sugars in the aerial parts of F.viridis.
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This study is conducted in the area from Shalateen-Halayeb in South East of Egypt. This study aimed to maintain and organize the uses of wild medicinal plants and also, to maximize the economic returns of these plants in this region... more
This study is conducted in the area from Shalateen-Halayeb in South East of Egypt. This study aimed to maintain and organize the uses of wild medicinal plants and also, to maximize the economic returns of these plants in this region through the definition and identification of the important species and the best methods for sustainable use of these plants including their distribution, density, productivity and their active materials. Forty six stands representing 13 localities were selected to study the distribution, habitats, density, productivity, chemical constituents, and active materials of the wild medicinal plants in the study area. These plants are distributed in eight habitats. The highest value of density of the medicinal plants was recorded in Zygophyllum simplex, followed by Rumex vesicarius and Chenopodium murale. Rhizophora mucronata had the highest value of productivity, followed by Avicenna marina and Lycium shawii, respectively. The ash contents ranged from 3.34% in Balanities aegyptiaca to 32.86% in Salvadora persica. For tannins, which is present in all plant samples under investigations, the highest value was observed in R. mucronata (5.178%), while the lowest value was detected in Plantago afra (0.007%). Saponins were detected in sixteen species. These Plants contained phenolic contents which ranged from 5.284% as highest value in R. mucronata to 0.039 in Leptadenia pyrotechnica. The highest concentration of alkaloid (10.943%) was detected in Z. simplex while the lowest one was detected in R. mucronata with percentages of 0.02%. Nine plant species contained oil with different ranges of concentrations.
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amples of the 26 plants for the study were freshly obtained from the southeastern of Egypt, packed in paper bags and brought to the laboratory for further processing. 70% ethanolic extracts were prepared and subjected to phytochemical... more
amples of the 26 plants for the study were freshly obtained from the southeastern of Egypt, packed in paper bags and brought to the laboratory for further processing. 70% ethanolic extracts were prepared and subjected to phytochemical analysis of their secondary metabolites, both qualitatively (alkaloids, terpenoids, tannins, saponins, flavonoids and phenolics) and quantitatively (alkaloids, phenolics and flavonoids), in addition to elemental analysis. The qualitative evaluation showed the presence of all investigated phytocompound classes in most of the studied plants. Quantitative analysis revealed that the highest phenolics content (20.44 g %) was in Acacia tortilis Forssk. and the highest flavonoids content (14.26 g %) was in Matthiola arabica Boiss. Zygophyllum simplex L. highly shared with 10.94% in alkaloids pool. Elemental profile for each plant was attained. The interest in the current investigation of these 26 medicinal plants was motivated by exploring new biologically active compounds resource.
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A pot experiment was carried out at the Experimental Farm of the Environment and Bio Agriculture Dept., Fac. of Agric., Al-Azhar University, Cairo, Egypt on 2016 to study the effect of salinity levels on growth and physiological... more
A pot experiment was carried out at the Experimental Farm of the Environment and Bio Agriculture Dept., Fac. of Agric., Al-Azhar University, Cairo, Egypt on 2016 to study the effect of salinity levels on growth and physiological characteristics of Atriplex canescens. Five NaCl concentrations (0, 150, 300, 450 and 600 mM) were tested under chemical and organic fertilization conditions. The seedlings were grown for 3 months after transplanting. Plant height, chlorophyll and leaf relative water content were measured at 30, 60 and 90 days. Proline, total carbohydrates, plant fresh and dry weight and protein contents were also measured to determine plants tolerance to salinity. The results revealed that low levels of salinity caused a slight inhibition on growth, but the higher concentrations reduced the shoot length, protein content and plant fresh and dry weight. Additionally, leaf chlorophyll content was declined by increasing NaCl concentrations. Proline and carbohydrate contents were increased with increasing NaCl concentration compared to control treatment. Potassium concentrations were decreased with increasing salinity since Na + and Cl-were increased.
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The high percentage of water content in aerial parts of Convolvulus althaeoides and Convolvulus stachydifolius var. villosus observed in leaf which can be affected the metabolic processes in plant. The percentage of total carbohydrates... more
The high percentage of water content in aerial parts of Convolvulus althaeoides and Convolvulus stachydifolius var. villosus observed in leaf which can be affected the metabolic processes in plant. The percentage of total carbohydrates and total lipid of Convolvulus althaeoides and Convolvulus stachydifolius var. villosus plants reached its maximum values in leaf decreasing gradually in flower to reach the lowest percentage in stem. Investigation of the free sugars showed that, galactose have maximum values in flower, galactose and glucose in leaf, glucose and galactose in stem in both plant respectively. The highest amount of total nitrogen and protein content was recorded in winter. The percentage of total nitrogen of Convolvulus althaeoides and Convolvulus stachydifolius var. villosus plants reached its maximum values in flower decreasing gradually in leaf to reach the lowest percentage stem; amino acid analyzer showed that, plants contains 15 free amino acids, respectively. Successive extraction showed that, methanol extracts have maximum values in flower, water extract have maximum values in leaf and stem respectively. Antimicrobial studies by used 6 bacterial strain and 5 fungal strains showed that, Convolvulus althaeoides and Convolvulus stachydifolius var. villosus plants can be used as antimicrobial agents, no Anti-virulence activity in both plant under investigation. [Ahmed FA, Sharaf AMA, Sofy MR and Elhaw MH. Phytochemical Analysis and Biological Activity of Convolvulus althaeoides and Convolvulus stachydifolius var. villosus. Rep Opinion 2014;6(7):36-44]. (ISSN: 1553-9873). http://www.sciencepub.net/report. 6
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The percentage of total phenolic acids, flavonoids, tannins, saponins and alkaloids of Convolvulus althaeoides and Convolvulus stachydifolius var. villosus aerial parts (flower, leaf and stem) were calculated by specificity methods.... more
The percentage of total phenolic acids, flavonoids, tannins, saponins and alkaloids of Convolvulus althaeoides and Convolvulus stachydifolius var. villosus aerial parts (flower, leaf and stem) were calculated by specificity methods. Chromatographic methods revealed the separation of twenty eight biologically active constituents from Convolvulus althaeoides and Convolvulus stachydifolius var. villosus. Identification of the chemical composition as well as the physico-chemical properties of the twenty eight active substances was carried out using ultraviolet, 1 H-NMR and 13 C-NMR spectral data , the separated compounds are; Gallic acid, Vitexin, Robinin (kamferol 3-O-Robinoside-7-O-rhamnoside, 7-hydroxyl flavone, 5-hydroxyl flavone, Alizarin,
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This study investigated the in vitro and in vivo anti-hepatocellular carcinoma activity of pseurotin A isolated from the n-butanol extract of Aspergillus fumigatus Fr. AUMC 8002 (nBE-AF).The in vitro anticancer activity of nBE-AF was... more
This study investigated the in vitro and in vivo anti-hepatocellular carcinoma activity of pseurotin A isolated from the
n-butanol extract of Aspergillus fumigatus Fr. AUMC 8002 (nBE-AF).The in vitro anticancer activity of nBE-AF was measured
against a human hepatocellular carcinoma cell line (HepG2) using a sulforhodamine-B (SRB) assay. The intraperitoneal median
lethal dose (LD50) of nBE-AF was determined in rats. Hepatocellular carcinoma was induced in rats by a single intraperitoneal
injection of diethylnitrosamine (DEN) (200 mg/kg b.wt.) followed by subcutaneous injections of carbon tetrachloride (CCl4) (3 ml/
kgb.wt.) weekly for 6 weeks. After administration of these carcinogens, 1/10 and 1/20 LD50 doses of nBE-AF were administered
intraperitoneally daily. NBE-AF exhibited significant cytotoxic activity against HepG2 cells. Administration of DEN and CCl4
significantly elevated the serum levels of liver function and tumour markers and significantly downregulated tumour necrosis
factor-α gene expression. Moreover, DEN and CCl4 decreased immunohistochemical Bax expression and increased Bcl-2
expression in the liver. Co-treatment with nBE-AF mitigated the DEN+CCl4-induced alterations in a dose-dependent manner.
Histopathological evaluation of the liver substantiated the above biochemical results. These results confirmed that nBE-AF,
via its major isolated secondary metabolite, pseurotin A, exerted an anti-hepatocarcinogenic effect and could be used as a
chemopreventive agent for hepatocellular carcinoma.awareness of the role of mycobacteria in infections associated with
exposure to aquarium fish
n-butanol extract of Aspergillus fumigatus Fr. AUMC 8002 (nBE-AF).The in vitro anticancer activity of nBE-AF was measured
against a human hepatocellular carcinoma cell line (HepG2) using a sulforhodamine-B (SRB) assay. The intraperitoneal median
lethal dose (LD50) of nBE-AF was determined in rats. Hepatocellular carcinoma was induced in rats by a single intraperitoneal
injection of diethylnitrosamine (DEN) (200 mg/kg b.wt.) followed by subcutaneous injections of carbon tetrachloride (CCl4) (3 ml/
kgb.wt.) weekly for 6 weeks. After administration of these carcinogens, 1/10 and 1/20 LD50 doses of nBE-AF were administered
intraperitoneally daily. NBE-AF exhibited significant cytotoxic activity against HepG2 cells. Administration of DEN and CCl4
significantly elevated the serum levels of liver function and tumour markers and significantly downregulated tumour necrosis
factor-α gene expression. Moreover, DEN and CCl4 decreased immunohistochemical Bax expression and increased Bcl-2
expression in the liver. Co-treatment with nBE-AF mitigated the DEN+CCl4-induced alterations in a dose-dependent manner.
Histopathological evaluation of the liver substantiated the above biochemical results. These results confirmed that nBE-AF,
via its major isolated secondary metabolite, pseurotin A, exerted an anti-hepatocarcinogenic effect and could be used as a
chemopreventive agent for hepatocellular carcinoma.awareness of the role of mycobacteria in infections associated with
exposure to aquarium fish
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The current study was to deal the isolation and identification of secondary metabolites from the stem of Ficus cordata Thunb. subsp. salicifolia (Vahl). The hespirdin was the highest concentration of flavonoid and E-vanillic acid was the... more
The current study was to deal the isolation and identification of secondary metabolites from the stem of Ficus cordata Thunb. subsp. salicifolia (Vahl). The hespirdin was the highest concentration of flavonoid and E-vanillic acid was the highest concentration of phenolic acid in the stem extract by using HPLC. The ethanolic extract of the plant stem was fractionated and separated to obtain the isolated compounds by different chromatographic techniques. Structures of these compounds were elucidated by R f-values, UV, 1 H-NMR, 13 C-NMR spectral analysis and compared with the literature data. Seven secondary metabolites were isolated from stem of Ficus cordata Thunb. subsp. salicifolia (Vahl); five flavonoid compounds were identified as 5, 7 dihydroxy flavanone, pinocembrin, rutin, apigenin, quercetin, luteolin-7-O-glucoside and two phenolic compounds were identified as gallic acid and ferulic acid.
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Eleven phenolic compounds [(1) ferulic acid, (2) Quercitrin, (3) Astralgalin, (4) Cynaroside, (5) Epicatechin, (6) Gallic acid, (7) Ellgaic acid, (8) Quercetin, (9) Kaempferol, (10) Luteolin and (11) 6-Hydroxy-4'methoxy aurone] were... more
Eleven phenolic compounds [(1) ferulic acid, (2) Quercitrin, (3) Astralgalin, (4) Cynaroside, (5) Epicatechin, (6)
Gallic acid, (7) Ellgaic acid, (8) Quercetin, (9) Kaempferol, (10) Luteolin and (11) 6-Hydroxy-4'methoxy
aurone] were isolated and identified from the 70% methanolic extract of T.ehrenbergii Schewienf .ex Boiss. aerial
parts by using different chromatographic methods. The acute toxicity examination for aqueous methanolic extract
of T.ehrenbergii aerial parts clearly indicated that, the extract is non-toxic and safe in rats up to 4500 mg/kg b.wt.
So, two specific doses (450 and 225 mg/kg) were used for hepatoprotective activity of T.ehrenbergii aerial parts
extract. Results show a potent hepatoprotective activity. It alleviated significantly the toxic effect of carbon
tetrachloride in rats by improvement of different biochemical parameters compared with carbon tetrachloride
treated group. The methanolic extract showed a potent cytotoxicity against liver carcinoma cell line (HEPG-2) at
concentration 38.4 μg/ml and lung carcinoma cell (A-549) at concentration 55.6 μg/ml
Gallic acid, (7) Ellgaic acid, (8) Quercetin, (9) Kaempferol, (10) Luteolin and (11) 6-Hydroxy-4'methoxy
aurone] were isolated and identified from the 70% methanolic extract of T.ehrenbergii Schewienf .ex Boiss. aerial
parts by using different chromatographic methods. The acute toxicity examination for aqueous methanolic extract
of T.ehrenbergii aerial parts clearly indicated that, the extract is non-toxic and safe in rats up to 4500 mg/kg b.wt.
So, two specific doses (450 and 225 mg/kg) were used for hepatoprotective activity of T.ehrenbergii aerial parts
extract. Results show a potent hepatoprotective activity. It alleviated significantly the toxic effect of carbon
tetrachloride in rats by improvement of different biochemical parameters compared with carbon tetrachloride
treated group. The methanolic extract showed a potent cytotoxicity against liver carcinoma cell line (HEPG-2) at
concentration 38.4 μg/ml and lung carcinoma cell (A-549) at concentration 55.6 μg/ml
The high percentage of water content of Lycium shawii Roem. & Shult. leaves and stem observed in winter season affected the metabolic processes in plant. The percentage of total carbohydrates of L. shawii plant reached its maximum values... more
The high percentage of water content of Lycium shawii Roem. & Shult. leaves and stem observed in winter season affected the metabolic processes in plant. The percentage of total carbohydrates of L. shawii plant reached its maximum values in summer decreasing gradually in spring to reach the lowest percentage leaves in autumn. Investigation of the free sugars showed that, plant leaves contained arabinose, ribose, fructose, galactose, glucose and sucrose, while plant stem was deficient in ribose and sucrose. The highest amount of total nitrogen and protein content was recorded in winter. Amino acid analyzer showed that, L. shawii plant contains 16 and 15 free amino acids, in addition to 15 and 16 amino acids in protein hydrolysates in leaves and stem, respectively. GLC analysis revealed that, L. shawii plant contains 10 fatty acids: 8 saturated and 2 unsaturated as well as 10 hydrocarbons beside three sterols for both leaves & stem. The percentage of total flavonoids, tannins, saponins and alkaloids increased in plant leaves than stem. Chromatographic methods revealed the separation of nine biologically active constituents from L. shawii leaves. Identification of the chemical composition as well as the physico-chemical properties of the nine active substances was carried out using ultraviolet, 1 H-NMR, 13 C-NMR spectral data and mass spectroscopy, the separated compounds are; 2, 3 dihydroxy benzoic acid, quercetin, gallic acid, rutin, p-coumaric acid, ferulic acid, quercetin 3-methoxy glucoside, quercetin 3, 7 diglucoside and quercetin 3-O-β-glucoside.
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There is currently an upsurge of interest in phytochemicals as a new source of natural antioxidants to be used in foods and pharmaceutical preparations to replace synthetic antioxidants, which are being restricted due to their potential... more
There is currently an upsurge of interest in phytochemicals as a new source of natural antioxidants to be used in foods and pharmaceutical preparations to replace synthetic antioxidants, which are being restricted due to their potential health risks and toxicity. The objective of the present study was to evaluate the phytochemical composition as well as the antioxidant activity of the ethanolic extracts of Ficus cordata Thunb. subsp. salicifolia (Vahl) leaves & stem on testicular tissue that has been exposed to oxidative stress by lead acetate in albino rats. Phytochemical screening revealed the presence of various bioactive secondary metabolites as flavonoids, phenolics, coumarins, terpenes, tannins, and sterols compounds which might be responsible for their medicinal attributes. Anthraquinones and volatile oils were not detected in leaves and stem. The quantitative estimation of total flavonoids and total phenolics present in higher amount in stem than leaves. LDR 50 R was calculated for leaves and stem extracts and was 3600 & 4600 mg/kg b.w., respectively. The oral administration of stem extract with a dose (460 mg kgP −1 P) reducing the oxidative stress in testicular tissue by increasing superoxide dismutase (SOD) and glutathione peroxidase (GPx).