omayma ragab
Benha University, Clinical biochemistry and molecular biology, Department Member
Abstract Prolonged exposure to different occupational or environmental toxicants triggered oxidative stress and inflammatory reactions mediated lung damage. This study was designed to explore the influence and protective impact of flavone... more
Abstract
Prolonged exposure to different occupational or environmental toxicants triggered oxidative stress and inflammatory
reactions mediated lung damage. This study was designed to explore the influence and protective impact of flavone on lung
injury in rats intoxicated with nicotine (NIC) and exposed to radiation (IR). Forty rats were divided into four groups; group
I control, group II flavone; rats were administered with flavone (25 mg/kg/day), group III NIC + IR; rats were injected
intraperitoneally with NIC (1 mg/kg/day) and exposed to γ-IR (3.5 Gy once/week for 2 weeks) while group IV NIC + IR +
flavone; rats were injected with NIC, exposed to IR and administered with flavone. Redox status parameters and histopathological changes in lung tissue were evaluated. Nuclear factor-kappa B (NF-κB), forkhead box O-class1 (FoxO1) and
nucleotide-binding domain- (NOD-) like receptor pyrin domain-containing-3 (NLRP3) gene expression were measured in
lung tissues. Moreover, nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and phosphatidylinositol three kinase (PI3K) were
measured using ELISA kits. Our data demonstrates, for the first time, that flavone protects the lung from NIC/IR-associated
cytotoxicity, by attenuating the disrupted redox status and aggravating the antioxidant defence mechanism via activation of
the PI3K/Nrf2. Moreover, flavone alleviates pulmonary inflammation by inhibiting the inflammatory signaling pathway
FOXO1/NF-κB/NLRP3- Inflammasome. Collectively, the obtained results exhibited a notable efficiency of flavone in
alleviating lung injury induced by NIC and IR via modulating PI3K/Nrf2 and FoxO1/NLRP3 Inflammasome.
Keywords
Nicotine, radiation, lung, PI3K/Nrf2, FoxO1/NLRP3, flavone
Prolonged exposure to different occupational or environmental toxicants triggered oxidative stress and inflammatory
reactions mediated lung damage. This study was designed to explore the influence and protective impact of flavone on lung
injury in rats intoxicated with nicotine (NIC) and exposed to radiation (IR). Forty rats were divided into four groups; group
I control, group II flavone; rats were administered with flavone (25 mg/kg/day), group III NIC + IR; rats were injected
intraperitoneally with NIC (1 mg/kg/day) and exposed to γ-IR (3.5 Gy once/week for 2 weeks) while group IV NIC + IR +
flavone; rats were injected with NIC, exposed to IR and administered with flavone. Redox status parameters and histopathological changes in lung tissue were evaluated. Nuclear factor-kappa B (NF-κB), forkhead box O-class1 (FoxO1) and
nucleotide-binding domain- (NOD-) like receptor pyrin domain-containing-3 (NLRP3) gene expression were measured in
lung tissues. Moreover, nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and phosphatidylinositol three kinase (PI3K) were
measured using ELISA kits. Our data demonstrates, for the first time, that flavone protects the lung from NIC/IR-associated
cytotoxicity, by attenuating the disrupted redox status and aggravating the antioxidant defence mechanism via activation of
the PI3K/Nrf2. Moreover, flavone alleviates pulmonary inflammation by inhibiting the inflammatory signaling pathway
FOXO1/NF-κB/NLRP3- Inflammasome. Collectively, the obtained results exhibited a notable efficiency of flavone in
alleviating lung injury induced by NIC and IR via modulating PI3K/Nrf2 and FoxO1/NLRP3 Inflammasome.
Keywords
Nicotine, radiation, lung, PI3K/Nrf2, FoxO1/NLRP3, flavone
Research Interests:
Abstract Prolonged exposure to different occupational or environmental toxicants triggered oxidative stress and inflammatory reactions mediated lung damage. This study was designed to explore the influence and protective impact of flavone... more
Abstract
Prolonged exposure to different occupational or environmental toxicants triggered oxidative stress and inflammatory
reactions mediated lung damage. This study was designed to explore the influence and protective impact of flavone on lung
injury in rats intoxicated with nicotine (NIC) and exposed to radiation (IR). Forty rats were divided into four groups; group
I control, group II flavone; rats were administered with flavone (25 mg/kg/day), group III NIC + IR; rats were injected
intraperitoneally with NIC (1 mg/kg/day) and exposed to γ-IR (3.5 Gy once/week for 2 weeks) while group IV NIC + IR +
flavone; rats were injected with NIC, exposed to IR and administered with flavone. Redox status parameters and histopathological changes in lung tissue were evaluated. Nuclear factor-kappa B (NF-κB), forkhead box O-class1 (FoxO1) and
nucleotide-binding domain- (NOD-) like receptor pyrin domain-containing-3 (NLRP3) gene expression were measured in
lung tissues. Moreover, nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and phosphatidylinositol three kinase (PI3K) were
measured using ELISA kits. Our data demonstrates, for the first time, that flavone protects the lung from NIC/IR-associated
cytotoxicity, by attenuating the disrupted redox status and aggravating the antioxidant defence mechanism via activation of
the PI3K/Nrf2. Moreover, flavone alleviates pulmonary inflammation by inhibiting the inflammatory signaling pathway
FOXO1/NF-κB/NLRP3- Inflammasome. Collectively, the obtained results exhibited a notable efficiency of flavone in
alleviating lung injury induced by NIC and IR via modulating PI3K/Nrf2 and FoxO1/NLRP3 Inflammasome.
Keywords
Nicotine, radiation, lung, PI3K/Nrf2, FoxO1/NLRP3, flavone
Prolonged exposure to different occupational or environmental toxicants triggered oxidative stress and inflammatory
reactions mediated lung damage. This study was designed to explore the influence and protective impact of flavone on lung
injury in rats intoxicated with nicotine (NIC) and exposed to radiation (IR). Forty rats were divided into four groups; group
I control, group II flavone; rats were administered with flavone (25 mg/kg/day), group III NIC + IR; rats were injected
intraperitoneally with NIC (1 mg/kg/day) and exposed to γ-IR (3.5 Gy once/week for 2 weeks) while group IV NIC + IR +
flavone; rats were injected with NIC, exposed to IR and administered with flavone. Redox status parameters and histopathological changes in lung tissue were evaluated. Nuclear factor-kappa B (NF-κB), forkhead box O-class1 (FoxO1) and
nucleotide-binding domain- (NOD-) like receptor pyrin domain-containing-3 (NLRP3) gene expression were measured in
lung tissues. Moreover, nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and phosphatidylinositol three kinase (PI3K) were
measured using ELISA kits. Our data demonstrates, for the first time, that flavone protects the lung from NIC/IR-associated
cytotoxicity, by attenuating the disrupted redox status and aggravating the antioxidant defence mechanism via activation of
the PI3K/Nrf2. Moreover, flavone alleviates pulmonary inflammation by inhibiting the inflammatory signaling pathway
FOXO1/NF-κB/NLRP3- Inflammasome. Collectively, the obtained results exhibited a notable efficiency of flavone in
alleviating lung injury induced by NIC and IR via modulating PI3K/Nrf2 and FoxO1/NLRP3 Inflammasome.
Keywords
Nicotine, radiation, lung, PI3K/Nrf2, FoxO1/NLRP3, flavone
Research Interests:
Purpose: Oral diethylnitrosamine (DEN) is a known hepatocarcinogen that damages the liver and causes cancer. DEN damages the liver through reactive oxygen species-mediated inflammation and biological process regulation. Materials and... more
Purpose: Oral diethylnitrosamine (DEN) is a known hepatocarcinogen that damages the liver and causes cancer.
DEN damages the liver through reactive oxygen species-mediated inflammation and biological process
regulation.
Materials and methods: Gallic acid-coated zinc oxide nanoparticles (Zn-GANPs) were made from zinc oxide (ZnO)
synthesized by irradiation dose of 50 kGy utilizing a Co-60 γ-ray source chamber with a dose rate of 0.83 kGy/h
and gallic acid from pomegranate peel. UV–visible (UV) spectrophotometry verified Zn-GANP synthesis. TEM,
DLS, and FTIR were utilized to investigate ZnO-NPs’ characteristics.
Rats were orally exposed to DEN for 8 weeks at 20 mg/kg five times per week, followed by intraperitoneal injection of Zn-GANPs at 20 mg/kg for 5 weeks. Using oxidative stress, anti-inflammatory, liver function, histologic, apoptotic, and cell cycle parameters for evaluating Zn-GANPs treatment.
Results: DEN exposure elevated inflammatory markers (AFP and NF-κB p65), transaminases (AST, ALT), γ-GT,
globulin, and total bilirubin, with reduced protein and albumin levels. It also increased MDA levels, oxidative
liver cell damage, and Bcl-2, while decreasing caspase-3 and antioxidants like GSH, and CAT. Zn-GANPs
significantly mitigated these effects and lowered lipid peroxidation, AST, ALT, and γ-GT levels, significantly
increased CAT and GSH levels (p<0.05). Zn-GANPs caused S and G2/M cell cycle arrest and G0/G1 apoptosis.
These results were associated with higher caspase-3 levels and lower Bcl-2 and TGF-β1 levels. Zn-GANPs enhance
and restore the histology and ultrastructure of the liver in DEN-induced rats.
Conclusion: The data imply that Zn-GANPs may prevent and treat DEN-induced liver damage and carcinogenesis.
DEN damages the liver through reactive oxygen species-mediated inflammation and biological process
regulation.
Materials and methods: Gallic acid-coated zinc oxide nanoparticles (Zn-GANPs) were made from zinc oxide (ZnO)
synthesized by irradiation dose of 50 kGy utilizing a Co-60 γ-ray source chamber with a dose rate of 0.83 kGy/h
and gallic acid from pomegranate peel. UV–visible (UV) spectrophotometry verified Zn-GANP synthesis. TEM,
DLS, and FTIR were utilized to investigate ZnO-NPs’ characteristics.
Rats were orally exposed to DEN for 8 weeks at 20 mg/kg five times per week, followed by intraperitoneal injection of Zn-GANPs at 20 mg/kg for 5 weeks. Using oxidative stress, anti-inflammatory, liver function, histologic, apoptotic, and cell cycle parameters for evaluating Zn-GANPs treatment.
Results: DEN exposure elevated inflammatory markers (AFP and NF-κB p65), transaminases (AST, ALT), γ-GT,
globulin, and total bilirubin, with reduced protein and albumin levels. It also increased MDA levels, oxidative
liver cell damage, and Bcl-2, while decreasing caspase-3 and antioxidants like GSH, and CAT. Zn-GANPs
significantly mitigated these effects and lowered lipid peroxidation, AST, ALT, and γ-GT levels, significantly
increased CAT and GSH levels (p<0.05). Zn-GANPs caused S and G2/M cell cycle arrest and G0/G1 apoptosis.
These results were associated with higher caspase-3 levels and lower Bcl-2 and TGF-β1 levels. Zn-GANPs enhance
and restore the histology and ultrastructure of the liver in DEN-induced rats.
Conclusion: The data imply that Zn-GANPs may prevent and treat DEN-induced liver damage and carcinogenesis.
Research Interests:
Background Melissa officinalis (MO) is a well-known medicinal plant species used in the treatment of several diseases; it is widely used as a vegetable, adding flavour to dishes. This study was designed to evaluate the therapeutic... more
Background Melissa officinalis (MO) is a well-known medicinal plant species used in the treatment of several
diseases; it is widely used as a vegetable, adding flavour to dishes. This study was designed to evaluate the therapeutic
effect of MO Extract against hyperthyroidism induced by Eltroxin and γ-radiation.
Methods Hyperthyroidism was induced by injecting rats with Eltroxin (100 µg/kg/ day) for 14 days and exposure
to γ-radiation (IR) (5 Gy single dose). The hyperthyroid rats were orally treated with MO extract (75 mg/kg/day) at
the beginning of the second week of the Eltroxin injection and continued for another week. The levels of thyroid
hormones, liver enzymes and proteins besides the impaired hepatic redox status and antioxidant parameters were
measured using commercial kits. The hepatic gene expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and
its inhibitor Kelch-like ECH-associated protein-1(Keap-1) in addition to hepatic inflammatory mediators including
tumor necrosis factor-α (TNF- α), Monocyte chemoattractant protein-1 (MCP-1) and fibrogenic markers such as
transforming growth factor-beta1 (TGF-β1) were determined.
Results MO Extract reversed the effect of Eltroxin + IR on rats and attenuated the thyroid hormones. Moreover, it
alleviated hyperthyroidism-induced hepatic damage by inhibiting the hepatic enzymes’ activities as well as enhancing
the production of proteins concomitant with improving cellular redox homeostasis by attenuating the deranged
redox balance and modulating the Nrf2/Keap-1 pathway. Additionally, MO Extract alleviated the inflammatory
response by suppressing the TNF- α and MCP-1 and prevented hepatic fibrosis via Nrf2-mediated inhibition of the
TGF-β1/Smad pathway.
Conclusion Accordingly, these results might strengthen the hepatoprotective effect of MO Extract in a rat model of
hyperthyroidism by regulating the Nrf-2/ Keap-1 pathway.
diseases; it is widely used as a vegetable, adding flavour to dishes. This study was designed to evaluate the therapeutic
effect of MO Extract against hyperthyroidism induced by Eltroxin and γ-radiation.
Methods Hyperthyroidism was induced by injecting rats with Eltroxin (100 µg/kg/ day) for 14 days and exposure
to γ-radiation (IR) (5 Gy single dose). The hyperthyroid rats were orally treated with MO extract (75 mg/kg/day) at
the beginning of the second week of the Eltroxin injection and continued for another week. The levels of thyroid
hormones, liver enzymes and proteins besides the impaired hepatic redox status and antioxidant parameters were
measured using commercial kits. The hepatic gene expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and
its inhibitor Kelch-like ECH-associated protein-1(Keap-1) in addition to hepatic inflammatory mediators including
tumor necrosis factor-α (TNF- α), Monocyte chemoattractant protein-1 (MCP-1) and fibrogenic markers such as
transforming growth factor-beta1 (TGF-β1) were determined.
Results MO Extract reversed the effect of Eltroxin + IR on rats and attenuated the thyroid hormones. Moreover, it
alleviated hyperthyroidism-induced hepatic damage by inhibiting the hepatic enzymes’ activities as well as enhancing
the production of proteins concomitant with improving cellular redox homeostasis by attenuating the deranged
redox balance and modulating the Nrf2/Keap-1 pathway. Additionally, MO Extract alleviated the inflammatory
response by suppressing the TNF- α and MCP-1 and prevented hepatic fibrosis via Nrf2-mediated inhibition of the
TGF-β1/Smad pathway.
Conclusion Accordingly, these results might strengthen the hepatoprotective effect of MO Extract in a rat model of
hyperthyroidism by regulating the Nrf-2/ Keap-1 pathway.
Research Interests:
Introduction: The clinical use of 5-fluorouracil (5-FU), a routinely used chemotherapy medication, has a deleterious impact ontheliver. Therefore, it is necessary to find a less harmful alternative to minimize liver damage. This study... more
Introduction: The clinical use of 5-fluorouracil (5-FU), a routinely used chemotherapy medication, has a deleterious impact
ontheliver. Therefore, it is necessary to find a less harmful alternative to minimize liver damage. This study was designed to
see how5-fluorouracil nanogel influenced 5-FU-induced liver damage in rats. Methods: To induce liver damage, male albino
rats were injected intraperitoneally with 5-FU (12.5 mg/kg) three doses/week for 1 month. The histopathological ex
amination together with measuring the activities of serum alanine and aspartate aminotransferase enzymes (ALT and AST)
were used to evaluate the severity of liver damage besides, hepatic oxidative stress and antioxidant markers were also
measured. The hepatic gene expression of heme oxygenase-1 (HO-1), nuclear factor erythroid 2-related factor 2 (Nrf2)
and its inhibitor Kelch-like ECH-associated protein-1(Keap-1) in addition to hepatic inflammatory mediators including
tumor necrosis factor-α (TNF- α) and interleukins (IL-1β, IL-6) were detected. Results: 5-Fu nanogel effectively attenuated
5-FU-induced liver injury by improving the hepatic structure and function (ALT and AST) besides the suppression of the
hepatic inflammatory mediators (TNF- α, IL-1β and IL-6). Additionally, 5-FU nanogel alleviated the impaired redox status
and restored the antioxidant system via maintaining the cellular homeostasis Keap-1/Nrf2/HO-1 pathway. Conclusion:
Consequently, 5-Fu nanogel exhibited lower liver toxicity compared to 5-FU, likely due to the alleviation of hepatic
inflammation and the regulation of the cellular redox pathway.
Keywords
hepatotoxicity, 5-fluorouracil, 5-FU nanogel, Nrf-2, HO-1
ontheliver. Therefore, it is necessary to find a less harmful alternative to minimize liver damage. This study was designed to
see how5-fluorouracil nanogel influenced 5-FU-induced liver damage in rats. Methods: To induce liver damage, male albino
rats were injected intraperitoneally with 5-FU (12.5 mg/kg) three doses/week for 1 month. The histopathological ex
amination together with measuring the activities of serum alanine and aspartate aminotransferase enzymes (ALT and AST)
were used to evaluate the severity of liver damage besides, hepatic oxidative stress and antioxidant markers were also
measured. The hepatic gene expression of heme oxygenase-1 (HO-1), nuclear factor erythroid 2-related factor 2 (Nrf2)
and its inhibitor Kelch-like ECH-associated protein-1(Keap-1) in addition to hepatic inflammatory mediators including
tumor necrosis factor-α (TNF- α) and interleukins (IL-1β, IL-6) were detected. Results: 5-Fu nanogel effectively attenuated
5-FU-induced liver injury by improving the hepatic structure and function (ALT and AST) besides the suppression of the
hepatic inflammatory mediators (TNF- α, IL-1β and IL-6). Additionally, 5-FU nanogel alleviated the impaired redox status
and restored the antioxidant system via maintaining the cellular homeostasis Keap-1/Nrf2/HO-1 pathway. Conclusion:
Consequently, 5-Fu nanogel exhibited lower liver toxicity compared to 5-FU, likely due to the alleviation of hepatic
inflammation and the regulation of the cellular redox pathway.
Keywords
hepatotoxicity, 5-fluorouracil, 5-FU nanogel, Nrf-2, HO-1
Research Interests:
3 Abstract Fluoride and aluminum are ubiquitous toxic metals with adverse reproductive efects. The citrus favonoid hesperidin has protective activities but poor solubility and bioavailability. Nanoparticulate delivery systems can improve... more
3
Abstract
Fluoride and aluminum are ubiquitous toxic metals with adverse reproductive efects. The citrus favonoid hesperidin has
protective activities but poor solubility and bioavailability. Nanoparticulate delivery systems can improve favonoid efectiveness. We conducted this study to prepare a pH-responsive chitosan-based nanogel for hesperidin delivery and evaluate its
efectiveness against sodium fuoride (NaF) and aluminum chloride (AlCl3) induced testicular toxicity in mice. The nanogel
was synthesized using 2 kGy gamma irradiation, enabling a size under 200 nm and enhanced hesperidin release at pH 6
matching testicular acidity. Male mice received 200 mg/kg AlCl3 and 10 mg/kg NaF daily for 30 days. Hesperidin nanogel
at 20 mg/kg was administered orally either prophylactically (pretreatment) or after intoxication (posttreatment). The results
showed that AlCl3+NaF induced severe oxidative stress, hormonal disturbance, apoptosis, and endoplasmic reticulum stress,
evidenced by signifcant changes in the studied parameters and testicular histological damage. Hesperidin nanogel administration signifcantly inhibited oxidative stress markers, restored luteinizing hormone (LH), follicle-stimulating hormone
(FSH), and testosterone levels, and alleviated tissue damage compared to the intoxicated group. It also downregulated the
expression level of pro-apoptotic genes Bax, caspase-3, caspase-9, and P38MAPK, while upregulating the expression level
of the anti-apoptotic BCL2 gene. Endoplasmic reticulum stress sensors PERK, ATF6, and IRE-α were also downregulated
by the nanogel. The chitosan-based nanogel enhanced the delivery and efcacy of poorly bioavailable hesperidin, exhibiting remarkable protective efects against AlCl3 and NaF reproductive toxicity. This innovative nanosystem represents a
promising approach to harnessing bioactive phytochemicals with delivery challenges, enabling protective efects against
chemical-induced testicular damage.
Keywords Hesperidin · Nanogel · Aluminum · Fluoride · Testicular damage
Abstract
Fluoride and aluminum are ubiquitous toxic metals with adverse reproductive efects. The citrus favonoid hesperidin has
protective activities but poor solubility and bioavailability. Nanoparticulate delivery systems can improve favonoid efectiveness. We conducted this study to prepare a pH-responsive chitosan-based nanogel for hesperidin delivery and evaluate its
efectiveness against sodium fuoride (NaF) and aluminum chloride (AlCl3) induced testicular toxicity in mice. The nanogel
was synthesized using 2 kGy gamma irradiation, enabling a size under 200 nm and enhanced hesperidin release at pH 6
matching testicular acidity. Male mice received 200 mg/kg AlCl3 and 10 mg/kg NaF daily for 30 days. Hesperidin nanogel
at 20 mg/kg was administered orally either prophylactically (pretreatment) or after intoxication (posttreatment). The results
showed that AlCl3+NaF induced severe oxidative stress, hormonal disturbance, apoptosis, and endoplasmic reticulum stress,
evidenced by signifcant changes in the studied parameters and testicular histological damage. Hesperidin nanogel administration signifcantly inhibited oxidative stress markers, restored luteinizing hormone (LH), follicle-stimulating hormone
(FSH), and testosterone levels, and alleviated tissue damage compared to the intoxicated group. It also downregulated the
expression level of pro-apoptotic genes Bax, caspase-3, caspase-9, and P38MAPK, while upregulating the expression level
of the anti-apoptotic BCL2 gene. Endoplasmic reticulum stress sensors PERK, ATF6, and IRE-α were also downregulated
by the nanogel. The chitosan-based nanogel enhanced the delivery and efcacy of poorly bioavailable hesperidin, exhibiting remarkable protective efects against AlCl3 and NaF reproductive toxicity. This innovative nanosystem represents a
promising approach to harnessing bioactive phytochemicals with delivery challenges, enabling protective efects against
chemical-induced testicular damage.
Keywords Hesperidin · Nanogel · Aluminum · Fluoride · Testicular damage
Research Interests:
Prolonged exposure to environmental aluminum-containing substances is associated with the development of Alzheimer's disease (AD). AD is a brain disorder associated with a gradual weakening in neurocognitive functions. Mesenchymal stem... more
Prolonged exposure to environmental aluminum-containing substances is associated with the development of Alzheimer's disease (AD). AD is a brain disorder associated with a gradual weakening in neurocognitive functions. Mesenchymal stem cells (MSCs) transplant as a promising and safe approach is used to treat AD through countless mechanisms. Therefore, this study aims to elucidate how MSCs improve biochemical and histopathological approaches associated with the AD model in rats. MSCs treatment restores the redox status impairment through a notable decline in the malondialdehyde (MDA) levels along with antioxidant enrichment. The anti-inflammatory effect of MSCs through conversion of microglial cells from M1 to M2 and inhibition of pro-inflammatory mediator’s release work in with de-activated GSK-3β. Additionally, the alleviation of autophagy and lysosomal clearance of Aβ and tau aggregates was accompanied by a down-regulation of the mTOR. Moreover, MSCs upregulate the expression of SIRT1 together with a limited expression of miR-134 thereby, improve neurite outgrowth and synaptic loss. Overall, the obtained data confirm the novelty of MSCs in the treatment of AD not only by their antioxidant, anti-inflammatory effect but also by restoring the neural integrity, neurogenesis, improving the neurocognitive function, and modulation of the signal pathways linked to the Aβ hypothesis. Key words: Aluminium, Alzheimer’s disease, mesenchymal stem cells, amyloid β, SIRT1, miR-134. Abbreviations: Al: Aluminium; AD: Alzheimer’s disease; Aβ: amyloid β; MSCs: mesenchymal stem cells; SIRT: Sirtuin; AlCl3: Aluminum chloride; MDA: malondialdehyde; CAT: catalase; H2O2: hydrogen peroxide; GSH: glutathione; IL-1β: Interleukin- 1β; ANOVA: One-way analysis of variance; LSD: Least Significant Difference; SE: standard error; STAT3: signal transducer and activator of transcription; PI3K: phosphatidylinositol 3 kinase; GSK3β: glycogen synthase kinase 3 beta;
Research Interests:
The use of 5-fluorouracil (5-FU) is associated with multifaceted challenges and poor pharmacokinetics. Accordingly, our study was designed to prepare 5-FU nanogel as a new form of the colon cancer chemotherapeutic drug 5-FU using... more
The use of 5-fluorouracil (5-FU) is associated with multifaceted challenges and poor pharmacokinetics. Accordingly, our study was designed to prepare 5-FU nanogel as a new form of the colon cancer chemotherapeutic drug 5-FU using polyacrylic acid and gelatin hybrid nanogel as efficient drug carriers. Alongside the in vivo chemotherapeutic evaluation, the anti-proliferative and anti-apoptotic efficacy were carried out for 5-FU nanogel against 1,2-dimethylhydrazine (DMH, 20 mg/kg) and γ-radiation (4 Gy)-prompted colon dysplasia in rats compared to 5-FU. The morphology and size of 5-FU nanogel were characterized by transmission electron microscopy (TEM) and dynamic light scattering (DLS) in addition to cytotoxicity assay. The expression of phosphoinositide-3-kinase (PI3K)/Akt, mammalian target of rapamycin (mTOR); Toll-like receptor2 (TLR2)/nuclear factor kappa B), adenosine monophosphate (AMP)-activated protein kinase (AMPK) and its downstream autophagy-related genes in addition to apoptotic markers were measured in colon tissues. Results: 5-FU nanogel reduced the levels of the TLR2/ NF-κβ as well as the expression of PI3K/AKT/mTOR. Moreover, it promoted autophagy through the activation of the AMPK and its downstream targets which consequently augmented the intrinsic and extrinsic apoptotic pathways. Conclusion: Collectively, these data might strengthen the therapeutic potential of 5-FU nanogel which can be used as an antitumor product for colon cancer.
Keywords 5-fluorouracil nanogel · Colon cancer · Autophagy · TLR2 · NF-κβ · PI3K · AKT · mTO
Keywords 5-fluorouracil nanogel · Colon cancer · Autophagy · TLR2 · NF-κβ · PI3K · AKT · mTO
Research Interests:
Nonalcoholic fatty liver disease (NAFLD) is the most prevalent chronic hepatic disorder. The naturally occurring phytosterol; β-sitosterol has antiobesogenic and anti-diabetic properties. The purpose of this study was to explore the role... more
Nonalcoholic fatty liver disease (NAFLD) is the most prevalent chronic hepatic disorder. The naturally occurring phytosterol; β-sitosterol has antiobesogenic and anti-diabetic properties. The purpose of this study was to explore the role of β-sitosterol in preventing hepatic steatosis induced by a high-fat diet (HFD) in rats. In the current study, to induce NAFLD in the female Wister rats, an HFD was administered to them for 8 weeks. The pathogenic severity of steatosis in rats receiving an HFD diet was dramatically decreased by oral administration of β-sitosterol. After administering β-sitosterol to HFD-induced steatosis for three weeks, several oxidative stress-related markers were then assessed. We showed that β-sitosterol reduced steatosis and the serum levels of triglycerides, transaminases (ALT and AST) and inflammatory markers (IL-1β and iNOS) compared to HFD-fed rats. Additionally, β-sitosterol reduced endoplasmic reticulum stress by preventing the overexpression of inositol-requiring enzyme-1 (IRE1α), X-box binding protein 1(sXBP1) and C/EBP homologous protein (CHOP) genes which, showing a function in the homeostatic regulation of protein folding. Also, it was found that the expression of the lipogenic factors; peroxisome proliferator-activated receptor (PPAR-α), sterol regulatory element binding protein (SREBP-1c) and carnitine palmitoyltransferase-1(CPT-1), which are involved in the regulation of the fatty acid oxidation process, may be regulated by β-sitosterol. It can be concluded that β-sitosterol may prevent NAFLD by reducing oxidative stress, endoplasmic reticulum stress and inflammatory responses, which supports the possibility of using β-sitosterol as an alternative therapy for NAFLD. Together, β-sitosterol may be an option for NAFLD prevention. Keywords NAFLD, β-sitosterol, Endoplasmic reticulum stress, PPAR-α, SREBP-1c, CPT-1
Research Interests:
The purpose of this study was to demonstrate the neuroprotective effect of Melissa officinalis extract (MEE) against brain damage associated with hypothyroidism induced by propylthiouracil (PTU) and/or γ-radiation (IR) in rats.... more
The purpose of this study was to demonstrate the neuroprotective effect of Melissa officinalis extract (MEE) against brain damage associated with hypothyroidism induced by propylthiouracil (PTU) and/or γ-radiation (IR) in rats. Hypothyroidism induction and/or exposure to IR resulted in a significant decrease in the serum levels of T3 and T4 associated with increased levels of lipid peroxidation end product, malondialdehyde (MDA), and nitrites (NO) in the brain tissue homogenate. Also, hypothyroidism and /or exposure to IR markedly enhance the endoplasmic reticulum stress by upregulating the gene expressions of the protein kinase RNA-like endoplasmic reticulum kinase (PERK), activated transcription factor 6 (ATF6), endoplasmic reticulum-associated degradation (ERAD), and CCAAT/enhancer-binding protein homologous protein (CHOP) in the brain tissue homogenate associated with a proapoptotic state which indicated by the overexpression of Bax, BCl2, and caspase-12 that culminates in brain damage. Meanwhile, the PTU and /or IR-exposed rats treated with MEE reduced oxidative stress and ERAD through ATF6. Also, the MEE treatment prevented the Bax and caspase-12 gene expression from increasing. This treatment in hypothyroid animals was associated with neuronal protection as indicated by the downregulation in the gene expressions of the microtubule-associated protein tau (MAPT) and amyloid precursor protein (APP) in the brain tissue. Furthermore, the administration of MEE ameliorates the histological structure of brain tissue. In conclusion, MEE might prevent hypothyroidism-induced brain damage associated with oxidative stress and endoplasmic reticulum stress. Keywords Hypothyroidism · Melissa officinalis · γ-radiation · Brain damage · Endoplasmic reticulum stress · ERAD
Research Interests:
Prolonged exposure to environmental aluminum-containing substances is associated with the development of Alzheimer's disease (AD). AD is a brain disorder associated with a gradual weakening in neurocognitive functions. Mesenchymal stem... more
Prolonged exposure to environmental aluminum-containing substances is associated with the development of Alzheimer's disease (AD). AD is a brain disorder associated with a gradual weakening in neurocognitive functions. Mesenchymal stem cells (MSCs) transplant as a promising and safe approach is used to treat AD through countless mechanisms. Therefore, this study aims to elucidate how MSCs improve biochemical and histopathological approaches associated with the AD model in rats. MSCs treatment restores the redox status impairment through a notable decline in the malondialdehyde (MDA) levels along with antioxidant enrichment. The anti-inflammatory effect of MSCs through conversion of microglial cells from M1 to M2 and inhibition of pro-inflammatory mediator’s release work in with de-activated GSK-3β. Additionally, the alleviation of autophagy and lysosomal clearance of Aβ and tau aggregates was accompanied by a down-regulation of the mTOR. Moreover, MSCs upregulate the expression of SIRT1 together with a limited expression of miR-134 thereby, improve neurite outgrowth and synaptic loss. Overall, the obtained data confirm the novelty of MSCs in the treatment of AD not only by their antioxidant, anti-inflammatory effect but also by restoring the neural integrity, neurogenesis, improving the neurocognitive function, and modulation of the signal pathways linked to the Aβ hypothesis. Key words: Aluminium, Alzheimer’s disease, mesenchymal stem cells, amyloid β, SIRT1, miR-134.
Research Interests:
This research evaluates the biochemical effect of adding 7% olive cake as feed additives on lipid profile, fatty acids, antioxidants concentrations in serum and/or egg yolk and on gene expression of fatty acid synthase and acetyl CoA... more
This research evaluates the biochemical effect of adding 7% olive cake as feed additives on lipid profile, fatty acids, antioxidants concentrations in serum and/or egg yolk and on gene expression of fatty acid synthase and acetyl CoA carboxylase in laying hens. The experiment was applied on 42 Commercial Mandarah strain laying hens which divided equally into control and olive cake (7%) group. Addition of olive cake (7%) led to a significant increase in serum and egg yolk HDL-C and a significant decrease in TAG, cholesterol, LDL-C and VLDL concentrations. SOD, GSH and TAC concentrations were significantly increased in serum and egg yolk in olive cake group, while MDA and NO were significantly decreased. Hens fed 7% olive cake recorded a significant increase in the concentration of oleic, linoleic and linolenic with a significant decrease in stearic in egg yolk. In breast muscle the concentration of oleic acid was significantly increased while saturated concentrations fatty acids were significantly decreased in olive cake group. Gene expression of FAS and ACC was significantly increased in the olive cake group. Keywords | Olive cake, FAS, ACC, Antioxidants, Fatty acids, Egg yolk
Research Interests:
In this study, potential protective and curative effect of probiotics on acetic acid-induced ulcerative colitis were evaluated. Ulcerative colitis was induced in rats by 2 ml of 3% glacial acetic acid (v/v) administered intracolonially... more
In this study, potential protective and curative effect of probiotics on acetic acid-induced ulcerative colitis were evaluated. Ulcerative colitis was induced in rats by 2 ml of 3% glacial acetic acid (v/v) administered intracolonially for 3 successive days. Forty male albino rats were divided into Five equal groups. Group I: (Control group) rats received no drugs. Group II: Early UC-induced group, Group III: Late UC-induced group, Group IV: (Early UC + probiotics protected) rats received probiotics (135 mg/kg b. wt/day) orally for 21 days prior to acetic acid administration then sacrificed. Group V: (Late UC + probiotics treated) rats first administered with acetic acid then after 3 days probiotics was received for 21 days. A significant increase in L-MDA and Myeloperoxidase (MOP) and up-regulation of IL-1β, caspase-3 with down-regulation in Bcl-2 gene expression as well as marked decrease in GSH and CAT were observed in colon tissue of UC-induced rats. However, administration of probiotics to UC-induced rats caused a marked improvement and reverse all previous parameters near the average range. On conclusion, Probiotics protect colonic mucosa against ulcerative colitis and may be effective in enhances the healing of ulcerated colon via anti-inflammatory and anti-apoptotic activities and regenerating endogenous antioxidant mechanism.
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The initiation and progression of nonalcoholic fatty liver disease (NAFLD) include imbalanced lipid metabolism, insulin resistance (IR) and oxidative stress. Consequantly, grape seeds proanthocyanidins extract (GSPE) have lipolytic... more
The initiation and progression of nonalcoholic fatty liver disease (NAFLD) include imbalanced lipid metabolism, insulin resistance (IR) and oxidative stress. Consequantly, grape seeds proanthocyanidins extract (GSPE) have lipolytic properties and showed very positive effects against the NAFLD metabolic disruptions. The present study aim to investigate the antioxidant and anti-inflammation effects of treatment of GSPE alone or in combination with antiplatelet drug (Clopidogrel) against high fat diet (HFD)-induced NAFL, in which 32 male albino rats were assigned into four equal groups of 8 rats: 1. Normal control group (G1) of normal standard-feeding rats for 12 weeks, 2. NAFL group (G2) of HFD-induced NAFL rats during 6 weeks, followed by normal feeding for another 6 weeks, 3. GSPE treated group (G3) of the NAFL rats followed by GSPE treatment for another 6 weeks, and 4. GSPE and clopidogrel co-treated group (G4) of the NAFL rats followed by GSPE and clopidogrel co-treatment for other 6 weeks. The results showed that treatment with GSPE alone or with clopidogrel significantly decreased the serum levels of total cholesterol, triglycerides (TG) and ferritin (G3,G4) that were significantly increased due to NAFL induction (G2), and significantly downregulated the liver tissue inflammatory mediators VEGF, PDGFα, and MAPK gene expressions (G3,4)that were significantly upregulated by NAFL induction (G2). In conclusion, treatment with GSPE alone or with antiplatelet alleviates the hepatic oxidative stress and inflammation associated with HFD-induction of NAFL in rats.
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This study was designed to investigate the anti-diabetic effects of selenium nanoparticles (SeNPs) at dose 2 mg/kg body weight in streptozotocin (STZ) induced diabetes in rats at dose 50 mg/kg body weight. Were administered orally in... more
This study was designed to investigate the anti-diabetic effects of selenium nanoparticles (SeNPs) at dose 2 mg/kg body weight in streptozotocin (STZ) induced diabetes in rats at dose 50 mg/kg body weight. Were administered orally in normal and experimentally induced diabetic rats for 35 days and glibenclamide (Glib) at the dose rate of 20 mg/kg was used as a reference drug. 48 waster male rats divided into 6 equal groups each of 8 rats. Blood samples and pancreatic tissue were collected at the end of experimental. Animals either treated with SeNPs its results demonstrated that SeNPs could significantly decreased blood glucose levels, enhanced serum insulin concentration, Result showed decrease liver function as in alanine aminotransferase (ALT) and aspartate aminotransferase (AST), decrease cardiac function creatine kinase-MB (CK-MB). total cholesterol, triglyceride and low density lipoprotein (LDL) levels significantly decreased and high density lipoprotein (HDL) significantly inc...
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Alzheimer's disease (AD) is a brain disorder associated with a gradual weakening in neurocognitive functions, neuroinflammation, and impaired signaling pathways. Resveratrol (RSV) has neuroprotective properties, but with low... more
Alzheimer's disease (AD) is a brain disorder associated with a gradual weakening in neurocognitive functions, neuroinflammation, and impaired signaling pathways. Resveratrol (RSV) has neuroprotective properties, but with low bioavailability, and low solubility in vivo. Selenium (Se) is an essential micronutrient for brain function. Thus, this study aimed to evaluate the role of formulated RSV-Se nanoparticles (RSV-SeNPs) on neurochemical and histopathological approaches associated with the AD model in rats induced by Aluminum chloride (AlCl3) at a dose of 100 mg/kg/day for 60 days. RSV-SeNPs supplementation attenuates the impaired oxidative markers and mitochondrial dysfunction. The ameliorative effect of RSV-SeNPs on cholinergic deficits was associated with clearance of amyloid β (Aβ). Furthermore, activation of phosphatidylinositol 3 kinase (PI3K) deactivates glycogen synthase kinase 3 beta (GSK-3β)-mediated tau hyperphosphorylation. Additionally, RSV-SeNPs downregulate signal transducer and activator of transcription (STAT3) expression as well as interleukin-1β (IL-1β) levels, therefore alleviating neuroinflammation in AD. Moreover, RSV-SeNPs upregulate the expression of Sirtuin-1 (SIRT1) and lower that of microRNA-134, consequently increasing neurite outgrowth. Eventually, the obtained results showed that nano-formulation of resveratrol with selenium maximized the therapeutic potential of RSV against Alzheimer's disease not only by their antioxidant but also by anti-inflammatory effect improving the neurocognitive function and modulating the signaling pathways.
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CITATIONS 0 READS 7 3 authors: Some of the authors of this publication are also working on these related projects: 13. Coordinator of BIOSENSORS Agricult
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ABSTRACT Background and aim of the work: Antioxidants can reduce damage produced by low doses of radiation on living cells. This study was designed to investigate the effects of fermented soy milk (FSM) and low dose of gamma radiation on... more
ABSTRACT Background and aim of the work: Antioxidants can reduce damage produced by low doses of radiation on living cells. This study was designed to investigate the effects of fermented soy milk (FSM) and low dose of gamma radiation on carcinogenic effect of N-methyl-N-nitrosourea (MNU). Material and methods: Female rats were divided into 8 groups: group (1): control, group (2): injected with MNU, group (3): whole body exposed to low dose of gamma radiation (0.5 Gy), group (4): given FSM orally, group(5): given FSM and MNU, group (6): received MNU and exposed to gamma radiation,, group (7): given FSM, MNU and exposed to gamma radiation. Results: Fermented soy milk exerted significant, ameliorative effect on glutathione peroxidase, superoxide dismutase and catalase activities, lipid peroxidation and TNF-α levelin rats injected with MNU. Combined treatment of FSM and low dose of gamma radiation markedly elevated GSH level, ameliorated MNU effect on cell cycle phases Go/1, S, G2/M and induce apoptosis via activation of caspase-3.Conclusion: FSM consumption with exposure to low doses of gamma radiation reduced carcinogenesis and oxidative stress effects induced by MNU in the mammary tissues. [Omayma, A. Ragab; El-sonbaty, S. M.; Aziza, S. A. and Aboelftouh, A. E. Effect of Probiotic Fermented Soy Milk and Gamma Radiation on Nitrosourea-Induced Mammary Carcinogenesis Nat Sci 2013;11(11):35-42]. (ISSN: 1545-0740). http://www.sciencepub.net/nature. 7 1. Introduction: Breast cancer is the most frequently diagnosed cancer and the leading cause of cancer death in female worldwide. The significance of nutrition in protecting living organisms from the toxic effects of environmental carcinogens has gained increasing attention due to less toxicity and high efficacy against various diseases. The intake of soy and soy-based products is associated with a lower risk of several types of cancers, including breast cancer. There are many functional ingredients contained in soy foods such as soy protein, isoflavones, saponins, phytic acid, phytosterol, and phenolic acid. The chemopreventive effects of soybean and soy containing food products may be related to genistiein, daidzein and glycitein(1). Human have always been exposed to various natural sources of ionizing radiation emitted by the isotopes present in the earth&amp;#39;s crust, air, water and biosphere, and also originating from the outer space. In some parts of the globe the level of this natural background radiation is significantly higher than the world average with no adverse health effects. Today, people can be additionally exposed to &amp;quot; man-made &amp;quot; radiation delivered at high doses (e.g., during radiotherapy and radiation accidents as well as after detonations of nuclear weapons) or low doses (e.g.,
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The present study was conducted to evaluate the safety and / or the toxicity of acute dual propolis and ginger treatment. Different doses 1, 2, 3, 4 and 5 g/kg b.wt (w/w) of the dual composite were administrated as an aqueous solution via... more
The present study was conducted to evaluate the safety and / or the toxicity of acute dual propolis and ginger treatment. Different doses 1, 2, 3, 4 and 5 g/kg b.wt (w/w) of the dual composite were administrated as an aqueous solution via an oral gavage to female albino rats. No adverse effects or mortality were observed during the 72-hrs observation period. In addition, necropsy examination indicated no abnormalities in the main organs after administration of any dose. Further, histopathological, hematological indices (hemoglobin, red blood cells, hematocrite, and white blood cells) and plasma parameters included Alanine amino transferase (ALT) and aspartate amino transferase (AST) , total protein (TP) total cholesterol, triglycerides (TG), creatinine and urea, malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were assessed in the plasma of all rats after treatment with the limited oral dose 5 g/kg b.wt (w/w). There were no changes in h...
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A B S T R A C T The aim of the present study was to elucidate the possible biochemical changes in lipid metabolism and organ function after treatment with sildenafil in normal and hyperlipidemic albino rats. Albino rats were grouped into... more
A B S T R A C T The aim of the present study was to elucidate the possible biochemical changes in lipid metabolism and organ function after treatment with sildenafil in normal and hyperlipidemic albino rats. Albino rats were grouped into seven groups (n=10/group) with blood sampling on days 30, 45 and 60 of the experiment for biochemical analysis. Group-i and -ii were fed on normal and cholesterol (1%) and fat (2%)-enriched diets, respectively. Group-iii and -iv were fed on normal and cholesterol (1%) and fat (2%)-enriched diets, respectively received sildenafil (5.625 mg/kg b. wt., orally, daily) after 30 days from the start of the experiment. Group-v received same diet like group-ii and treated with sildenafil at the start of the experiment. Group-vi and -vii were fed same diet like group-ii but received atorvastatin (1.8 mg/kg b.wt, orally, daily) and ezetimibe (1 mg/kg b.wt.), respectively after 30 days from the start of the experiment. Sildenafil significantly decreased the ser...
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Sildenafil citrate (Viagra) is an oral medication used to treat male impotence by the inhibition of phosphodiesterase-5 in the corpus cavernosum and subsequent facilitation of penile erection. The side effects of sildenafil citrate have... more
Sildenafil citrate (Viagra) is an oral medication used to treat male impotence by the inhibition of phosphodiesterase-5 in the corpus cavernosum and subsequent facilitation of penile erection. The side effects of sildenafil citrate have been reported. The present study was designed to investigate the effect of long term administration of sildenafil citrate on some characteristics (sperm count, sperm malformations) and testis architecture of male rats. The results indicated that the oral administration of 50 mg and 100 mg of suldinafil citrate (Viagra) for 8 weeks caused a significant decreased and a significant increased in sperm count and sperm malformations respectively. The results were showed the histopathological changes in testis of male rats treated with 50 mg and 100 mg sildenafil citrate..
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This study was performed to investigate the effect of toxoplasmosis on several biochemical parameters in aborted women (aborted due to toxoplasma) 43 women were classified into four groups. The first group contains 10 women with full term... more
This study was performed to investigate the effect of toxoplasmosis on several biochemical parameters in aborted women (aborted due to toxoplasma) 43 women were classified into four groups. The first group contains 10 women with full term of pregnancy and normal delivers kept as control group. The second group composed of 16 women aborted one time the third group contain 10 women aborted for two time. Fourth group contain 7 women aborted for three time. Blood sample were collected from all women. The obtained result reveled that a significant increase of serum toxoplasma IgG, IgM and cortisol, on aborted groups (G2, G3 and G4) compared with control group (G1). While, there was none significance difference in cancer antigen 125 (CA125) in serum compared with control group. Also, Results showed a significant increase of immunoglobulin IgG, IgE, IgA, IL2 and IL6 and on aborted groups (G2, G3 and G4) compared with control group (G1). While, there was no significant difference of IgM between in group G4 compared with control group (G1).
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The hepatoprotective effect of basic curcumin and bis (tetrachlorocuprate -lysine) combined with ascorbate against ferric nitrilotriacetate (Fe-NTA) and chloroform induced hepatic carcinogenesis in rats was evaluated. One hundred rats... more
The hepatoprotective effect of basic curcumin and bis (tetrachlorocuprate -lysine) combined with ascorbate against ferric nitrilotriacetate (Fe-NTA) and chloroform induced hepatic carcinogenesis in rats was evaluated. One hundred rats were divided into five equal groups. Normal control group, carcinogenic (Fe-NTA: 9 mg Fe/kg b.wt. i.p.) and chloroform (150 mg/ kg b.wt. orally)-induced group, curcumin group (400 mg/kg. b.wt. orally), tetrachlorocuprate-lysine (25 mg/kg. b. wt. s.c.) and ascorbate (500 mg/kg. b. wt. orally) group and a mixture group (composed of curcumin, tetrachlorocuprate -lysine and ascorbate). Blood samples and liver tissue specimens were collected at the end of experiment (4 months) for determination of the following parameters: activities of serum malondialdehyde (MDA), reduced glutathione (GSH), tissue catalase, myeloperoxidase (MPO), Tumor necrosis factor-α (TNFα ), interleukin-1, nuclear factor kappaB65 (NFkB65) and caspase-3. The obtained results showed that injection of carcinogen caused a significant elevation in the MDA, MPO and immunological markers levels, and significant reduction in the reduced glutathione, and catalase compared to the control group. Compared to carcinogenic group, rats administered with the treated compounds resulted in a significant reduction of MDA, MPO and immunological markers levels, and increased in reduced glutathione and catalase activity. These results concluded that basic curcumin, tetrachlorocuprate-lysine and ascorbate exert chemopreventative effect against hepatocellular carcinoma.
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Expansion of efficacious theranostic systems is of pivotal significance for medicine and human healthcare. Magnetic nanoparticles (MNPs) are known as drug delivery system and magnetic resonance imaging (MRI) contrast agent. MNPs as drug... more
Expansion of efficacious theranostic systems is of pivotal significance for medicine and human healthcare. Magnetic nanoparticles (MNPs) are known as drug delivery system and magnetic resonance imaging (MRI) contrast agent. MNPs as drug carriers have attracted significant attention because of the delivery of drugs loaded onto MNPs to solid tumors, maintaining them in the target site by an external electromagnetic field, and subsequently releasing drugs in a controlled manner. On the other hand, it is believed that MNPs possess high potential as MRI contrast agents. The aim of this work was to payload curcumin into dextran coated MNPs and investigate their potential as theranostic systems for controlled drug delivery and MRI imaging. MNPs were synthesized as a core and coated with dextran as polymeric shell to provide steric stabilization. Curcumin as anticancer drug was selected to be loaded into NPs. To characterize the synthesized NPs, various techniques (e.g., DLS, FESEM, FT-IR, XRD, and VSM) were utilized. In vitro drug release of curcumin was evaluated at 37˚C at the pH value of 5.4 and 7.4.The feasibility of employment of dextran coated MNPs as MRI contrast agents were also studied. Formulations prepared from dextran coated MNPs showed high loading (13%) and encapsulation efficiency (95%). In vitro release study performed in the phosphate-buffered saline (PBS, pH= 7.4, 5.4) revealed that the dextran coated MNPs possess sustained release behavior at least for 4 days with the high extent of drug release in acidic media. Vibrating sample magnetometer (VSM) analysis proved the superparamagnetic properties of the dextran coated MNPs with relatively high-magnetization value indicating that they were sufficiently sensitive to external magnetic fields as magnetic drug carriers. Furthermore, dextran coated MNPs exhibited high potential as T2 contrast agents for MRI. Based on our findings, we propose the dextran coated MNPs as promising nanosystem for the delivery of various drugs such as curcumin and MRI contrast agent.
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ABSTRACT The immunosuppressive drug cyclosporine A (CsA) has been successfully used in several diseases with immunological basis and in transplant patients. Nephrotoxicity is the main secondary effect of CsA treatment.The present study... more
ABSTRACT The immunosuppressive drug cyclosporine A (CsA) has been successfully used in several diseases with immunological basis and in transplant patients. Nephrotoxicity is the main secondary effect of CsA treatment.The present study was designed to investigate the possible protective effect of dietary fish oil (F.O.) on CsA-induced nephrotoxicity in rats. Eighty male rats were divided into four equal groups. Group 1 rats received no drugs and served as control, group 2 normal rats were treated with (dietary fish oil) omega-3 fatty acids 270 mg/kg b.w oral dose daily, group 3 rats treated with CsA (25 mg/kg body weight, orally for 21 days) to induce nephrotoxicity, groups 4 rats received dietary fish oil for 21 days before, 21 days concurrently during CsA administration and 21 days later after nephrotoxicity induction. Blood samples for serum separation and kidney tissue specimens were collected three times at weekly interval from the last dose of CsA administration. Serum glucose, total Protein, albumin, lipid profile(total cholesterol, triacylglycerols and phospholipids), renal function tests( urea, uric acid and creatinine), electrolytes(sodium and potassium), inorganic phosphorus and haptoglobin levels, lactate dehydrogenase (LDH) and gamma glutamyl transferase(GGT) activities were determined. Moreover, kidney tissue malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide (NO), total antioxidant capacity(TAO) levels, antioxidant enzymes(catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities were also determined. The results revealed that, CsA-induced nephrotoxicity caused significant increase in serum glucose, renal functions tests, haptoglobin, lipid profiles and serum marker enzymes (LDH and GGT) with significant decrease in serum total protein, albumin and electrolytes concentrations which were reversed upon treatment with dietary fish oil. Also, CsA administration induced significant elevation in lipid peroxidation(MDA) along with significant decrease in antioxidant enzyme activities, non enzymatic antioxidant, total antioxidant capacity and nitric oxide level in the rat kidney. Meanwhile, Dietary fish oil administration improved renal function, by bringing about a significant decrease in peroxidative levels and increase in antioxidant status. These results indicate the renoprotective potential and usefulness of dietary fish oil, as an excellent source of antioxidants, in modulating CsA-induced nephrotoxicity.
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ABSTRACT A B S T R A C T Cyclosporine A (CsA) is a potent and effective immunosuppressive agent, but its use is frequently accompanied by severe renal toxicity. CsA-induced nephrotoxicity results from increased production of free radical... more
ABSTRACT A B S T R A C T Cyclosporine A (CsA) is a potent and effective immunosuppressive agent, but its use is frequently accompanied by severe renal toxicity. CsA-induced nephrotoxicity results from increased production of free radical species in the kidney. The present study was designed to investigate the possible protective effect of green tea extract (GTE) on CsA-induced nephrotoxicity in rats. Eighty male rats were divided into four equal groups. Group 1 rats not receive drugs or green tea and served as control, group 2 normal rats administrated with green tea extract (3% w/v) orally, group 3 rats supplied with CsA (25 mg/kg body weight, orally for 21 days) to induce nephrotoxicity, groups 4 rats received green tea extract for 21 days before, 21 days concurrently during CsA administration and 21 days later after nephrotoxicity induction. Blood samples for serum separation and kidney tissue specimens were collected three times at weekly interval from the last dose of CsA administration. Serum glucose, total cholesterol, triacylglycerols and phospholipids, urea, uric acid and creatinine, sodium and potassium, inorganic phosphorus, total Protein, albumin, and haptoglobin levels, lactate dehydrogenase (LDH) and gamma glutamyl transferase (GGT) activities were also determined. Moreover, kidney tissue malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide (NO), total antioxidant capacity (TAO) levels, antioxidant enzymes catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GP x) activities were also determined. The results revealed that CsA-induced nephrotoxicity caused asignificant increase in serum glucose, renal functions tests, lipid profiles and serum LDH and GGT activities with asignificant decrease in serum total protein, albumin and electrolytes concentrations which were reversed upon treatment with green tea extract. Also, CsA administration induced asignificant elevation in lipid peroxidation (MDA) along with a significant decrease in antioxidant enzyme activities, non-enzymatic antioxidant, total antioxidant capacity and nitric oxide level in the rat kidney. Meanwhile, green tea extract administration improved renal functions, by bringing about a a significant decrease in peroxidative levels and increase in antioxidant status. These results indicate the nephroprotective potential effect and usefulness of green tea extract, as an excellent source of antioxidants, in modulating CsA-induced nephrotoxicity.
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Adiponectin is an adipocytokine produced mainly from liver and adipose tissue and expressed in various tissues with multifunctional effects. Little is known about adiponectin expression and regulation in cattle and that is the purpose of... more
Adiponectin is an adipocytokine produced mainly from liver and adipose tissue and expressed in various tissues with multifunctional effects. Little is known about adiponectin expression and regulation in cattle and that is the purpose of this study. Using northern ...
The main objective of the present study is to investigate the ameliorative role of ferulic acid (FA) against acrylamide (ACR)-induced neurotoxicity in rats. Forty male Wister albino rats were divided into four equal groups, Group I... more
The main objective of the present study is to investigate the ameliorative role of ferulic acid (FA) against acrylamide (ACR)-induced neurotoxicity in rats. Forty male Wister albino rats were divided into four equal groups, Group I (control): received one ml per day physiological saline; Group II (FA): received FA 100 mg/kg body weight per day for 21 days; Group III (ACR): administrated 50 mg/kg body weight per day acrylamide for 21 days; Group IV (ACR+ FA): ACR was administrated as group III then FA was given as group II. ACR induced a significant decrease in Brain Derived Neurotrophic Factor (BDNF), Epinephrine (EPI), Norepinephrine (NE) and Serotonin (SER) in the brain tissue and significantly increases the level of β-amyloid (Aβ) and Acetylcholinestrase (AChE) in the brain tissue and in serum Interleukine-6 (IL-6) levels. FA has a potent antioxidant and anti-inflammatory properties that has protective effect against ACR-induced neurotoxicity. FA decreases the levels of Aβ, IL-6, and AChE and also increased the level of BDNF, EPI, NE and SER in the ACR treated group. Histopathological investigation of brain tissues after FA treatment partially supported the beneficial effect of FA. From the obtained data it can be concluded that, FA has a beneficial effect and anti-inflammatory agent against ACR induced-neurotoxicity.
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This study designed to investigate the anti-inflammatory effect of zinc oxide nanoparticles (ZnONPs) on inflammatory mediators in dextran sulfate sodium salt induced ulcerative colitis(UC) in rats .twinty-four albino rats divided into 4... more
This study designed to investigate the anti-inflammatory effect of zinc oxide nanoparticles (ZnONPs) on inflammatory mediators in dextran sulfate sodium salt induced ulcerative colitis(UC) in rats .twinty-four albino rats divided into 4 equal of eight rats each. Group 1: (normal control) received no drugs , group 2: (ulcerative colitis) rats received dextran sulfate sodium salt 3% in drinking water, group 3: (ZnONPs) rats administered ZnONPs (5mg/kg body weight) orally for 3weeks, group 4: (ZnONPs +UC) rats with ulcerative colitis orally treated with ZnONPs (5mg/kg body weight) daily for 3 weeks. The obtained results revealed that administration of ZnONPs to rats with ulcerative colitis have no change on serum total cholesterol and TG concentrations and markedly increased the reduced HDL-C level. On the other hand, elevated level of COX-2, IL-6, MDA and TNF-α in UC rats were significantly reduced, with significant increase of the reduced level of GSH. Results suggest that ZnONPs mod...
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Background: Anthracyclines such as doxorubicin are highly effective chemotherapy agents used to treat many common malignancies. However, their use is limited by cardiotoxicity. Rivaroxaban, a direct Factor Xa inhibitor, is now approved in... more
Background: Anthracyclines such as doxorubicin are highly effective chemotherapy agents used to treat many common malignancies. However, their use is limited by cardiotoxicity. Rivaroxaban, a direct Factor Xa inhibitor, is now approved in United States and elsewhere for the prevention of venous thrombo-embolism. Coenzyme Q is mitochondrial respiratory chain element. Ginger has staring potential for treating cardiovascular disorders (atherosclerosis and hypertension). Murraya koenigii is known as ‘curry patta’ has anti-oxidant, anti-tumor, anti-inflammatory effects. Aim of study: In order to evaluate the protective effect of rivaroxban, ubiquinone separated and combined in the form of liposomes, combined curry and ginger on the myocardial infarcted rat model induced by doxorubicin. In vivo study was carried out, free doxorubicin; different liposomal preparations were assessed by different measurements. Materials and methods: The tested groups received respectively, group (1) Control. (2) Group B:received doxorubicin. (3) Group Q: liposomal ubiquinone and doxorubicin.(4) Group R: liposomal rivaroxban and doxorubicin. (5) Group QR: liposomal rivaroxban and ubiquinone encapusulated on the same liposome and doxorubicin. (6) Group D: blend of ginger, curry and doxorubicin. Particles size, zeta potential and TEM were measured for liposomal preparations. Several parameters have been measured to assess the cardiotoxicity (Albumin, lactate dehydrogenase enzyme (LDH), Aspartate Aminotransferase (AST), Anticardiolipin IgG, Creatine kinase-MB (CK MB), C-reactive protein (CRP), Tumor necrosis factor (TNF α)) and histopathological studies. Results: Rats with free doxorubicin caused significantly elevated levels of all parameters measured compared to control group, contol group vs. dox. treated group was as follow at p<0.05 (albumin 4.435 ± 0.085 vs. 2.910 ± 0.10, anticardiolipin 9.4 ± 0.256 vs.19.31 ± 0.4391, AST 64.60 ± 1.79 vs. 244.8 ± 9.86, CK 14.04 ± 1.794 vs. 73.25 ± 1.240, CRP4.904 ± 0.45 vs. 23.96 ± 0.95, LDH 212 ± 10.1 vs. 754 ± 16.4, TNF α 34.62 ± 1.05 vs.126.6 ± 6.25). In addition there is a significant variation of other groups compared to group B. Conclusionc There is a marked cardiotoxicity of doxorubicin could be decreased by the administration of antithrombic rivaroxban, respiratory chain element ubiquinone, herbals like ginger and curry.
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In the present study, the potential therapeutic effect of bee venom administration diabetic rats was evaluated. Thirty male albino rats were divided into three equal groups of 10 rats each. Group Ι:(Control group): received no drugs.... more
In the present study, the potential therapeutic effect of bee venom administration diabetic rats was evaluated. Thirty male albino rats were divided into three equal groups of 10 rats each. Group Ι:(Control group): received no drugs. Group II:(Diabetic rats group): rats received a single dose of Streptozotocin (STZ) (50mg/kg-b.wt i.p). Group III:(Diabetic rats + bee venom treated group): rats are treated with + bee venom 0.5 mg /kg body weight /day, i.p) for 21 days after diabetes induction. Blood samples and pancreatic tissue were collected at the 22th day from the onset of bee venom administration. The obtained results showed that, STZ-induced diabetic rats exhibited a significant increase in serum glucose, triglycerides, total cholesterol, LDL-cholesterol, malondialdehyde (MDA), advanced glycation end products (AGEs) and glycated hemoglobin (HbA1c) with marked decrease in HDL-cholesterol, insulin levels, Catalase (CAT), glutathione peroxidase (GSH-px), superoxide dismutase (SOD)....
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This study was performed to investigate the effect of oral supplementation of curcumin, garlic extract and olive oil on lipid profile, nitric oxide, adiponectin, endothelin-1, blood glucose and some inflammatory markers in normal,... more
This study was performed to investigate the effect of oral supplementation of curcumin, garlic extract and olive oil on lipid profile, nitric oxide, adiponectin, endothelin-1, blood glucose and some inflammatory markers in normal, diabetic and hyperlipidemic rats supplementing high fat and cholesterol-enriched diet. Forty female adult albino rats were divided into four equal groups of 10 rats each. Group (1): negative control received normal diet only, group (2): rats fed on normal diet and received curcumin orally, group (3): positive control received hyperlipidemic diet, group (4): rats fed on hyperlipidemic diet and received curcumin (350 mg/ 1 kg b.w.) orally. The obtained results revealed that, curcumin supplementations to hyperlipidemic rats showed a significant increase in serum HDL-cholesterol, nitric oxide, adiponectin and Endothelin-1 concentrations and significantly decrease in serum total cholesterol, triacylglycerols, LDLcholesterol, Fasting blood glucose, Glycated Hemo...
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Cadmium (Cd) is a well-known hazardous environmental contaminant. Non-steroidal anti-inflammatory drugs (NSAIDs) have been reported to have a deleterious effect on liver and kidney. Therefore, in this study, we investigated whether NSAIDS... more
Cadmium (Cd) is a well-known hazardous environmental contaminant. Non-steroidal anti-inflammatory drugs (NSAIDs) have been reported to have a deleterious effect on liver and kidney. Therefore, in this study, we investigated whether NSAIDS could potentiate the toxic effect of Cd on liver and kidney. Twenty male Wister rats were divided randomly into 4 groups. Group 1:the control was injected saline daily. Group 2: received cadmium (5mg/kg) daily. Group 3: served as piroxicam were injected a single dose (10 mg/kg, i.p.). Group 4: received both Cd+Px. After 14 days, all rats were euthanized and blood samples, stomach, liver, and kidney tissues were collected. Treatment with Cd or Px alone significantly increased the liver and kidney function markers. However, when Cd and Px were given in a combination, Px potentiated the Cd-induced cellular damage in liver and kidney when compared to their individual treatment in liver and kidney functions and histopathological examination. Their combination could also enhance the apoptosis.
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The preventive effect of Spirulina platensis on hepatotoxicity induced by diethylnitrosamine (DEN) in albino rats was evaluated. Forty male albino rats were divided into four equal groups: group (1): normal control, group (2): hepatotoxic... more
The preventive effect of Spirulina platensis on hepatotoxicity induced by diethylnitrosamine (DEN) in albino rats was evaluated. Forty male albino rats were divided into four equal groups: group (1): normal control, group (2): hepatotoxic rats administrated DEN orally (20 mg/kg b.wt/ day), group (3): rats receive spirulina orally and group (4): Hepatotoxic rats treated with spirulina at a dose of (800 mg / kg bw/day). All animals were sacrificed at the end of experiment (12 weeks). The hepatotoxic effect of DEN was evidenced by elevation of serum Aspartate Aminotransferase, Alanine Aminotransferase, Alkaline Phosphatase, γ-Glutamyl transferase activities, blood Urea concentration and liver Malondialdehyde level. However serum albumin concentration and antioxidants biomarkers in liver tissue were markedly decreased. Thus, the present study indicated that, Spirulina platensis successfully prevented the hepatoxic effects of DEN via enhancement of antioxidant defense system, suppression of oxidative stress and inflammation against oxygen free radicals.
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Acute Pancreatitis (AP) is a multifactorial disease. It was characterized by severe inflammation and acinar cell destruction. Thus, the present study was initiated to evaluate the role the of Cinnamic acid nanoparticles (CA-NPs) as a... more
Acute Pancreatitis (AP) is a multifactorial disease. It was characterized by severe inflammation and acinar cell destruction. Thus, the present study was initiated to evaluate the role the of Cinnamic acid nanoparticles (CA-NPs) as a modulator for the redox signaling pathway involved in the development of pancreatitis. AP in rats was induced by L-arginine and exposure to gamma radiation. The pancreatic injury was evaluated using biochemical and histological parameters. Upon the oral administration of CA-NPs, both the severity of acute pancreatitis and the serum levels of amylase and lipase were decreased. Furthermore, the malondialdehyde (MDA) levels of the pancreatic tissue were significantly reduced and the depletion of glutathione was considerably restored. The injury and apoptosis of pancreatic tissues were markedly improved by the reduction of the caspase-3levels. Additionally, the alleviation of pancreatic oxidative damage by CA-NPs was accompanied by a down-regulation of the NLRP3, NF- κB, and ASK1/MAPK signaling pathways. Collectively, the current findings showed that CA-NPs could protect the pancreatic acinar cell from injury not only by its antioxidant, anti-inflammatory effect but also by modulation of the redox-sensitive signal transduction pathways contributed to acute pancreatitis severity. Accordingly, cinnamic acid nanoparticles have therapeutic potential for the management of acute pancreatitis.
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Acute pancreatitis (AP) is a localized inflammation of the pancreatic gland that often leads to local and systemic complications. Cinnamic acids have been identified as interesting compounds with antioxidant, anti-inflammatory and... more
Acute pancreatitis (AP) is a localized inflammation of the pancreatic gland that often leads to local and systemic complications. Cinnamic acids have been identified as interesting compounds with antioxidant, anti-inflammatory and cytotoxic properties. A pre-clinical study using L-arginine induced AP in the rat model was attempted to evaluate the antioxidant effect of Cinnamic acid. The result confirmed that the AP condition was developed in response to the injection of L-arginine causing significant changes in different pancreatic enzymes; amylase and lipase in addition to the oxidative stress biomarkers. The treatment with Cinnamic acid caused a marked effect on these investigated parameters. The findings of the present study demonstrated that Cinnamic acid provided effective protection against AP induced by L-arginine in rats since this compound was able to ameliorate serum enzymes released from pancreas, enzymatic and non-enzymatic antioxidant defense system and to prevent the lipid peroxidation in these tissues. More detail study about Cinnamic acid treatment reversing or reducing acute pancreatitis is needed.
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Objective: The present study was designed to investigate the anti-colitic effect of Proanthocyanidins (Pcs) on acetic acid -induced ulcerative colitis in rats. Methods: Forty male rats (220-250 g) were divided into five equal groups.... more
Objective: The present study was designed to investigate the anti-colitic effect of Proanthocyanidins (Pcs) on acetic acid -induced ulcerative colitis in rats. Methods: Forty male rats (220-250 g) were divided into five equal groups. Group Ι:(Control normal group) rats received no drugs. Group Π: (Early UC-induced group) rats received 3% acetic acid (2ml/rat) intracolonially and sacrificed 3 days later from induction. Group III:(Late UC –induced group) rats received acetic acid similar to group 2 and sacrificed after 21 days. Group IV:(Early UC+Pcs protected group) rats received Pcs (200 mg/kg body weight/day) orally for 21 days prior to acetic acid administration for UC induction. Group V:(Late UC+Pcs treated group) treated with Pcs as in group III for 21 days after UC induction. Results: A significant increase in L-MDA and MOP with marked decrease in GSH and CAT activity in colon of acetic acid -induced colitis in rats as compared with control group. However, a significant depletion of colon tissue L-MDA, MOP and marked increase in GSH and CAT activity were observed after Pcs treatment compared to ulcerated untreated rats. The qPCR results revealed a significant upregulation of mRNA gene expression levels of TNF-α, COX-2 and a significant downregulation in TGF-β1 in colon of acetic acid -induced UC in rats. The expression levels of TNF-α, COX-2 was significantly downregulated and a significant upregulated in TGF-β1 in colon tissues after administration of Pcs. Conclusions: Proanthocyanidins protects rats colon mucosa damage against acetic acid -induced ulcerative colitis via anti-inflammatory and anti-oxidative mechanisms.
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Hepatocellular carcinoma (HCC) is one of the main causes leading to cancer mortality. Luteolin derived from celery and has anti-cancer activity. Cisplatin is used as a chemotherapeutic agent for the treatment of HCC. A major problem with... more
Hepatocellular carcinoma (HCC) is one of the main causes leading to cancer mortality. Luteolin derived from celery and has anti-cancer activity. Cisplatin is used as a chemotherapeutic agent for the treatment of HCC. A major problem with Cisplatin is the development of Cisplatin chemo resistance. This study aims to evaluate the role of Luteolin in HCC treatment and as a chemo-sensitizer for Cisplatin treatment of HCC in rats. Sixty rats were divided in to sex groups, 10 rats each, group 1: normal control group, group 2: cisplatin (1.5 mg/Kg b.wt) for four weeks and group 3: received the Diethyl nitrosamine (DEN) (20 mg/kg b.wt), group 4: received DEN as in group 3 and then treated with cisplatin, group 5: received DEN as in group 3 and then treated with Luteolin (0.2 mg/kg b.wt) and group 6: received DEN as in group 3 and then treated with cisplatin and Luteolin. After 10 weeks animals were sacrificed and liver tissue were removed for Histopathological and tissue parameters examination. A significant increase in serum serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) activity, a significant decrease in serum albumin concentration. Also a significant increases in liver transforming growth factor-beta 1 (TGF-b1), B-cell lymphoma 2 (Bcl-2) and nuclear factor kappa β (NF-kβ) concentrations and a significant decrease in Caspase.3 activity were recorded in DEN-treated rats while treatment with Luteolin and Cisplatin reduced the severity of HCC and enhanced histopathological findings, these result suggesting the efficacy of Luteolin supplementation as an anti-HCC.
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Tobacco smoking and hepatic damage causes oxidative stress in humans and underlay numerous chronic degenerative diseases. Heavy smoking yields toxins which induce necroinflammation and increase the severity of hepatic lesions.... more
Tobacco smoking and hepatic damage causes oxidative stress in humans and underlay numerous chronic degenerative diseases. Heavy smoking yields toxins which induce necroinflammation and increase the severity of hepatic lesions. Accordingly, the main object of this study to investigate the effect of chrysin against thioacetamide (TAA)-induced liver injury in rats exposed to tobacco smoke in combination. The study was carried out by investigating the oxidative stress biomarkers (glutathione peroxidase, SOD, catalase, paroxynase-1 and NAD (P) H: quinoneoxidoreductase 1 activities), inflammatory markers (IL-6 and IL-8 levels and gene expressions of NF-κB and VEGF) as well as histopathogical examination. It was found a significant reduction in activities of antioxidant enzymes concomitant with cytokines elevation; (IL-6 and IL-8) in rats exposed to tobacco smoke in combination with (TAA) treatment compared with normal control. Additionally, the transcript levels of (NF-κB and VEGF) genes were significantly higher in the rats exposed to tobacco smoke in combination with (TAA) treatment compared with normal control. However, treatment with chrysin, the levels of these parameters were significantly decreased which demonstrates the ameliorative effect of chrysin against (TAA) induced liver injury in rats exposed to tobacco smoke in combination. Histological investigations revealed that chrysin gave more preventive effect against thioacetamide (TAA)-induced liver injury in rats exposed to tobacco smoke. In conclusion, chrysin modulated the damage induced by exposure to tobacco smoking and
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The discovery of Cisplatin triggered the development of novel drugs containing other metals. Extremely diverse structural Chemistry and interaction of metal with ligand resulted in exploration of novel metal complexes with drug potential.... more
The discovery of Cisplatin triggered the development of novel drugs containing other metals. Extremely diverse structural Chemistry and interaction of metal with ligand resulted in exploration of novel metal complexes with drug potential. The present work carried on 80 female rats which divided into four groups, after 12 weeks the mammary tumor appeared the possible anti-cancer effect of (Cadmium-N Acetyl Cysteine) which has been synthesized and study its effect on (Cd44, Cyclin D1, p21, caspase3, caspase 9 and cell cycle) were investigated on bearing solid mammary tumor induced by DMBA in rats. key words: Metal complexCD44-Cyclin D1-Caspase-ligand. 2018) , 45 2 232 ): (3 4 3 BVMJ ( ) http://www.bvmj.bu.edu.eg (
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One of the main methods of modern cancer treatment is drug therapy but, it is found to be difficult to cure effectively. N-acetylcysteine (NAC), which is a membrane-permeable metal-binding compound, might have anti-cancer activity in the... more
One of the main methods of modern cancer treatment is drug therapy but, it is found to be difficult to cure effectively. N-acetylcysteine (NAC), which is a membrane-permeable metal-binding compound, might have anti-cancer activity in the presence of different metals. In the present study synthesis of NAC with (Cu, Zn, Cd, Fe, Mg, Mn, Cr, Ni, Co, Se and Ag) by metals investigation and the formation of these complexes by IR, NMR. The effect of cytotoxicity of these complexes on Breast, Liver, Prostate and Ovary human cancer cell lines were evaluated. The best complexes were seen in cadmium N acetyl cysteine complex in breast, ovary, prostate and liver cell line.
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Mesenchymal stem cells (MSCs) are recruited to the stroma of cancers. They interact with cancer cells to promote invasion and metastasis or to suppress tumor growth. The unique tumor-homing capacity of MSCs makes them a promising vehicle... more
Mesenchymal stem cells (MSCs) are recruited to the stroma of cancers. They interact with cancer cells to promote invasion and metastasis or to suppress tumor growth. The unique tumor-homing capacity of MSCs makes them a promising vehicle to deliver various anticancer agents. (http://www.bvmj.bu.edu.eg) (bvmj,34(1):369-374,Sept.,2018)
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Nanoparticles combined with natural products could be an efficient tool in cell base cancer gene therapy. Guided treatments with nanoparticles and radiotherapy are a new approach in cancer therapy. The current study evaluated the... more
Nanoparticles combined with natural products could be an efficient tool in cell base cancer gene therapy. Guided treatments with nanoparticles and radiotherapy are a new approach in cancer therapy. The current study evaluated the beneficial antitumor effects of SeNPs, γ-radiation together with selenium nanoparticles (SeNPs) against mammary gland cancer in female Rats. (http://www.bvmj.bu.edu.eg) (bvmj, 35(1): 362-368, March, 2018)
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Polycystic ovary syndrome (PCOS) is a set of symptoms due to elevated androgens\in females. Its Signs include irregular or no menstrual periods, heavy periods, excess body and facial hair, acne, pelvic pain, difficulty getting pregnant,... more
Polycystic ovary syndrome (PCOS) is a set of symptoms due to elevated androgens\in females. Its Signs include irregular or no menstrual periods, heavy periods, excess body and facial hair, acne, pelvic pain, difficulty getting pregnant, and patches of thick, darker, velvety skin. It could be a result from the combination of genetic and environmental factors. Many drugs used for treating this disease. Several hormones have been measured to evaluate PCOS (progesterone, Estradiol, Follicular Stimulating Hormone (FSH), Luteinizing hormone (LH), Total Testosterone, AntiMullerian Hormone (AMH), Thyroid Stimulating Hormone (TSH), Prolactin and histopathological studies. Groups were classified as follow: Group (1) Control, Group (2) Dehydroepiandrosterone (DHEA only), Group (3) (DHEA and flaxseed), Group (4) (DHEA and alfalfa), Group (5) (DHEA and Soya), Group (6) (DHEA and Cabergoline), and Group (7) (DHEA and Leuproline). Results: DHEA in a dose of (1mg/kg) induced PCOS and it was clear by significant elevation of (LH/ FSH ratio, total testosterone, AMH, TSH, Prolactin) and a significant decrease in progesterone and estradiol. Leuproline was the best choice to decrease the symptoms of PCOS followed by cabergoline and the least but still effective were the herbals (Soya, flaxseed, and alfalfa). In conclusion, DHEA could be used for PCOS induction, Leuproline, cabergoline and phytoestrogen containing herbals could be used for treating PCOS symptoms.
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The aim of this study was transforming doxorubin by encapsulating it in mesoporous silica compared to free doxorubicin. In vivo study, free doxorubicin, mesoporous silica preparations were assessed by different measurements like particles... more
The aim of this study was transforming doxorubin by encapsulating it in mesoporous silica compared to free doxorubicin. In vivo study, free doxorubicin, mesoporous silica preparations were assessed by different measurements like particles size, zeta potential and TEM. Several parameters have been measured to assess the cardiotoxicity (Albumin, lactate dehydrogenase (LDH), Aspartate Aminotransferase (AST), Anticardiolipin IgG, Creatine kinase-MB (CK MB), C-reactive protein (CRP), Tumor necrosis factor (TNF α)). The experimental animals were divided into seven groups as follow: group (1) Control. (2) Group B: received doxorubicin. (3) Group C: mesoporous doxorubicn. Rats injected with free doxorubicin caused significantly elevated levels of all parameters measured compared to control group (albumin 4.435 ± 0.085 vs 2.910 ± 0.10, anticardiolipin 9.4 ± 0.256 vs 19.31 ± 0.4391, AST 64.60 ± 1.79 vs 244.8 ± 9.86, CK 14.04± 1.794 vs 73.25± 1.240, CRP 4.904±0.45 vs 23.96 ± 0.95, LDH 212±10.1 vs 754±16.4, TNF α 34.62±1.05 vs 126.6±6.25). In addition there was a significant variation of mesoporous silica results compared to free doxorubicin. Conclusion: There was a marked cardiotoxicity of doxorubicin could be decreased by transforming it into mesoporous form.
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The present study was designed to evaluate the suspected anticancer activity of Solenostemma argel (200 mg/kg body weight/ day) either alone or combined with cisplatin (0.64 mg/kg body weight/day) for 4 weeks against lung cancer induced... more
The present study was designed to evaluate the suspected anticancer activity of Solenostemma argel (200 mg/kg body weight/ day) either alone or combined with cisplatin (0.64 mg/kg body weight/day) for 4 weeks against lung cancer induced by DMBA (20 mg/kg bw) for 8 weeks in male Wistar rats. At the end of experiment, blood and Lung samples were collected to assess biochemical and biophysical markers as well histopathological investigations. Biochemical parameters:, serum Tumor suppressor protein (P53) and (P16), Transforming Growth Factor-beta (TGF-β), interleukin1beta (IL-1β), interleukin-6 (IL-6), Carcino Embryonic antigen (CEA). Lung induced nitric oxide synthase (i-Nos), cyclin dependent kinase-2 (CDK2). The level of significance between mean values was set at p < 0.05. The results of this study also showed that administration of olenostemma argel to lung carcinoma induced rats demonstrated a significant improvement in biochemical parameters and life span as compared to DMBA control rats. The histopathological examinations of this study revealed damage and degeneration in the lung of DMBA treated rats. Also, lung of Argel and Cisplatin rats showed significant improvement and protection against DMBA harmful effect.
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The present study was designed to elucidate the protective effect of subsequent pretreatment with Fructus Piperis Longi extract in liver and brain tissue of experimental rats exposed to Hepatic encephalopathy induced by intraperitoneal... more
The present study was designed to elucidate the protective effect of subsequent pretreatment with Fructus Piperis Longi extract in liver and brain tissue of experimental rats exposed to Hepatic encephalopathy induced by intraperitoneal administration of TAA. Plf extract (50 mg/kg) was force-fed to rats every day, 7 days before administration of Thioacetamide. Thioacetamide 200 mg/kg was intraperitoneally administered to rats twice per week for four weeks. Administration of Plfext was extended during Thioacetamide. After 4 weeks, animals were sacrificed. Biochemical parameters in serum and homogenized liver and brain were tested. A significant increase in serum transglutaminase, alkaline phosphatase and gamma glutamyl transferase activity and plasma ammonia, a significant decrease in serum albumin and total protein was recorded in the liver of Thioacetamide-treated rats. Significant increases in liver and brain lipid peroxides associated to significant decreases of liver and brain reduced glutathione concentration and catalase activities were also recorded. Administration of Plfext treatment reduced the severity of liver fibrosis and oxidative damage which was substantiated by improvement of liver function detected by a decrease in serum aspartate amino transaminase, alanine aminotransferase, alkaline phosphatase, gamma glutamyl transferase activities and increase in total protein and Albumin. It was concluded that treatment of the ethanolic extract of Fructus Piperis Longi enhance Liver and brain parameter and enhanced antioxidant activities of liver and brain.