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FI903443A7 - FRAMSTAELLNING AV LACKAS GENOM RECOMBINANTORGANISMER. - Google Patents

FRAMSTAELLNING AV LACKAS GENOM RECOMBINANTORGANISMER.

Info

Publication number
FI903443A7
FI903443A7 FI903443A FI903443A FI903443A7 FI 903443 A7 FI903443 A7 FI 903443A7 FI 903443 A FI903443 A FI 903443A FI 903443 A FI903443 A FI 903443A FI 903443 A7 FI903443 A7 FI 903443A7
Authority
FI
Finland
Prior art keywords
laccase
enzyme
isolated
gene
produced
Prior art date
Application number
FI903443A
Other languages
Finnish (fi)
Other versions
FI903443A0 (en
Inventor
Markku Saloheimo
Marja-Leena Niku-Paavola
Merja Penttilae
Jonathan Knowles
Anne Kantelinen
Original Assignee
Valtion Teknillinen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Valtion Teknillinen filed Critical Valtion Teknillinen
Priority to FI903443A priority Critical patent/FI903443A7/en
Publication of FI903443A0 publication Critical patent/FI903443A0/en
Priority to PCT/FI1991/000214 priority patent/WO1992001046A1/en
Publication of FI903443A7 publication Critical patent/FI903443A7/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y110/00—Oxidoreductases acting on diphenols and related substances as donors (1.10)
    • C12Y110/03—Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
    • C12Y110/03002—Laccase (1.10.3.2)
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
    • C12N9/0061—Laccase (1.10.3.2)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Paper (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

This invention relates to production of a ligninolytic oxidative enzyme, laccase, by recombinant DNA technology in suitable hosts. For that purpose the laccase gene is isolated from a suitable donor organism, the gene is transformed to a suitable host, the recombinant organism obtained is cultivated in conditions allowing expression of laccase and the laccase so produced is isolated. Laccase can be used to improve for instance the pulping process due to the ability of the enzyme to degrade and modify lignin. A suitable donor organism for the invention is Phlebia. A preferable host for laccase expression for the purposes of the invention is the species Trichoderma. According to the invention also enzyme preparates can be produced by Trichoderma which besides laccase include also other enzymes useful in for instance pulp and paper processing.
FI903443A 1990-07-06 1990-07-06 FRAMSTAELLNING AV LACKAS GENOM RECOMBINANTORGANISMER. FI903443A7 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
FI903443A FI903443A7 (en) 1990-07-06 1990-07-06 FRAMSTAELLNING AV LACKAS GENOM RECOMBINANTORGANISMER.
PCT/FI1991/000214 WO1992001046A1 (en) 1990-07-06 1991-07-08 Laccase production by recombinant organisms

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FI903443A FI903443A7 (en) 1990-07-06 1990-07-06 FRAMSTAELLNING AV LACKAS GENOM RECOMBINANTORGANISMER.

Publications (2)

Publication Number Publication Date
FI903443A0 FI903443A0 (en) 1990-07-06
FI903443A7 true FI903443A7 (en) 1992-01-07

Family

ID=8530767

Family Applications (1)

Application Number Title Priority Date Filing Date
FI903443A FI903443A7 (en) 1990-07-06 1990-07-06 FRAMSTAELLNING AV LACKAS GENOM RECOMBINANTORGANISMER.

Country Status (2)

Country Link
FI (1) FI903443A7 (en)
WO (1) WO1992001046A1 (en)

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