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WO2026017636A1 - Compositions comprising combination of enzymes - Google Patents

Compositions comprising combination of enzymes

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Publication number
WO2026017636A1
WO2026017636A1 PCT/EP2025/070123 EP2025070123W WO2026017636A1 WO 2026017636 A1 WO2026017636 A1 WO 2026017636A1 EP 2025070123 W EP2025070123 W EP 2025070123W WO 2026017636 A1 WO2026017636 A1 WO 2026017636A1
Authority
WO
WIPO (PCT)
Prior art keywords
sequence identity
seq
mannanase
identity
protease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/EP2025/070123
Other languages
French (fr)
Inventor
Lisbeth Kalum
Iasminy DA SILVA BRASIL
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Publication of WO2026017636A1 publication Critical patent/WO2026017636A1/en
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38645—Preparations containing enzymes, e.g. protease or amylase containing cellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/244—Endo-1,3(4)-beta-glucanase (3.2.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/2488—Mannanases
    • C12N9/2494—Mannan endo-1,4-beta-mannosidase (3.2.1.78), i.e. endo-beta-mannanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01003—Triacylglycerol lipase (3.1.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/21—Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21)
    • C12Y301/21001—Deoxyribonuclease I (3.1.21.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01006—Endo-1,3(4)-beta-glucanase (3.2.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01078—Mannan endo-1,4-beta-mannosidase (3.2.1.78), i.e. endo-beta-mannanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01151—Xyloglucan-specific endo-beta-1,4-glucanase (3.2.1.151), i.e. endoxyloglucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01155—Xyloglucan-specific exo-beta-1,4-glucanase (3.2.1.155), i.e. exoxyloglucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
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  • Detergent Compositions (AREA)

Abstract

The present invention relates to enzyme compositions with improved cleaning and/or anti-redeposition effect.

Description

COMPOSITIONS COMPRISING COMBINATION OF ENZYMES
Reference to sequence listing
This application contains a Sequence Listing in computer readable form. The computer readable form is incorporated herein by reference.
FIELD OF THE INVENTION
The present invention relates to enzyme compositions comprising at least two enzymes and detergent compositions comprising said enzyme compositions providing the same or better wash performance compared to the individual enzymes.
BACKGROUND OF THE INVENTION
The ability of a detergent to remove stains from the surface of textiles is an obvious care- about for the consumer and various surfactant ingredients play a role in that process. However, there is a desire to improve the performance of the detergent compositions, lower the wash temperature and preferably at the same time reduce the amount of non-biological ingredients in the detergent composition. One reason for aiming at replacing classic detergent ingredients - such as polymers and surfactants - with enzymes is that some of the surfactant ingredients in detergents are derived from petrochemical resources and face scrutiny due to environmental concerns, most of all for not being sustainable because they are from a non-renewable source and are poorly biodegradable or even persistent in the environment. Another reason is compaction of detergent compositions: making compact detergent compositions reduces production cost, eases transportation, and ultimately means less burden on the environment, but reduction of surfactants also calls for the development of sustainable solutions that ensures continued performance of the detergent compositions.
SUMMARY OF THE INVENTION
Compaction of the detergents, improvement of performance, and the desire to replace detergent ingredients with active ingredients that are more sustainable calls for combining different enzymes to obtain synergistic effects that are even more effective than the ones on the market today, both in terms of stability of the enzymes, the ability to replace non-biological detergent ingredients with enzymes, improved stain removal and good performance at lower wash temperature and/or shorter wash cycles than traditionally used today. The inventors of the present invention have identified several new enzyme compositions that are useful for the above purposes. Accordingly, the present invention discloses new combinations of enzymes useful for textile and hard surface cleaning, detergent compositions comprising said enzyme compositions as well as methods for use of said enzyme compositions.
DEFINITIONS
In accordance with this detailed description, the following definitions apply. Note that the singular forms "a," "an," and "the" include plural references unless the context clearly dictates otherwise. Unless defined otherwise or clearly indicated by context, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
AEP (active enzyme protein): Enzyme protein which has a catalytic activity. There is various way to determine AEP. For example, AEP can be calculated by dividing total activities by the enzyme’s specific activity. The amount of enzyme is throughout the application considered to be active enzyme protein.
Amino acids: The polypeptides of the present invention comprise amino acids selected from the 20 natural amino acids. To represent the 20 natural amino acids the following nomenclature is used: alanine - ala - A, arginine - arg - R, asparagine - asn - N, aspartic acid - asp - D, cysteine - cys - C, glutamine - gin - Q, glutamic acid - glu - E, glycine - gly - G, histidine
- his - H, isoleucine - ile - 1, leucine - leu - L, lysine - lys - K, methionine - met - M, phenylalanine
- phe - F, proline - pro - P, serine - ser - S, threonine - thr - T, tryptophan - trp - W, tyrosine - tyr
- Y and valine - val - V.
Anti-pilling: The term "anti-pilling" denotes removal of pills from the textile surface and/or prevention of formation of pills on the textile surface.
Bacterial: The term “bacterial” in relation to polypeptide (such as an enzyme) refers to a polypeptide encoded by and thus directly derivable from the genome of a bacteria, where such bacteria has not been genetically modified to encode said polypeptide, e.g. by introducing the encoding sequence in the genome by recombinant DNA technology. In the context of the present invention, the term “bacterial enzyme” or “polypeptide having enzyme activity obtained from a bacterial source” or “polypeptide is of bacterial origin” thus refers to a enzyme encoded by and thus directly derivable from the genome of a bacterial species, where the bacterial species has not been subjected to a genetic modification introducing recombinant DNA encoding said enzyme. A sequence encoding a bacterial polypeptide having enzyme activity may also be referred to as a wildtype enzyme (or parent enzyme). Bacterial polypeptide having enzyme activity includes recombinant produced wild types. In a further aspect, the invention provides polypeptides having enzyme activity, wherein said polypeptides are substantially homologous to a bacterial enzyme. In the context of the present invention, the term “substantially homologous” denotes a polypeptide having enzyme activity which is at least 80%, preferably at least 85%, more preferably at least 90%, more preferably at least 95%, even more preferably at least 96%, 97%, 98%, and most preferably at least 99% identical to the amino acid sequence of a selected bacterial enzyme.
Biosurfactants: Biosurfactants are active compounds that are produced at the microbial cell surface or excreted and reduce surface and interfacial tension. Microbial surfactants offer several advantages over synthetic ones, such as low toxicity and high biodegradability, and often remain active at extreme pH and salinity. Biosurfactants are produced by bacteria, yeasts, and filamentous fungi and are generally classified into low molecular-mass molecules (lipopeptides, glycolipids) and high molecular-mass polymers (polymeric and particulate surfactants). Biosurfactants include but are not limited to rhamnolipids - in particular mono rhamnolipids - and sophorolipids.
Color clarification: During washing and wearing loose or broken fibers can accumulate on the surface of the fabrics. One consequence can be that the colors of the fabric appear less bright or less intense because of the surface contaminations. Removal of the loose or broken fibers from the textile will partly restore the original colors and looks of the textile. By the term "color clarification", as used herein, is meant the partial restoration the visual appearance of the initial colors of textile.
Detergent adjunct ingredient: The detergent adjunct ingredient is different to the proteases of this invention. The precise nature of these additional adjunct components, and levels of incorporation thereof, will depend on the physical form of the composition and the nature of the operation for which it is to be used. Suitable adjunct materials include, but are not limited to the components described below such as surfactants, builders, flocculating aid, chelating agents, dye transfer inhibitors, enzymes, enzyme stabilizers, enzyme inhibitors, in particular reversible protease inhibitors, catalytic materials, bleach activators, hydrogen peroxide, sources of hydrogen peroxide, preformed peracids, s, s, brighteners, suds suppressors, dyes, perfumes, structure elasticizing agents, fabric softeners, carriers, hydrotropes, builders and co-builders, fabric hueing agents, anti-foaming agents, dispersants, processing aids, solvents, and/or pigments.
Detergent composition: The term “detergent composition” refers to compositions that find use in the removal of undesired compounds from items to be cleaned, such as textiles. The detergent composition may be used to e.g., clean textiles for both household cleaning and industrial cleaning. The terms encompass any materials/compounds selected for the particular type of cleaning composition desired and the form of the product (e.g., liquid, gel, powder, granulate, paste, bar, or spray compositions) and includes, but is not limited to, detergent compositions (e.g., liquid and/or solid laundry detergents and fine fabric detergents; fabric fresheners; fabric softeners; laundry boosters; and textile and laundry pre-spotters/pre- treatment). In addition to containing the enzyme composition of the invention, the detergent formulation may contain one or more additional enzymes (such as proteases, amylases, lipases, cutinases, cellulases, endoglucanases, xyloglucanases, pectinases, pectin lyases, xanthanases, peroxidases, haloperoxygenases, catalases and mannanases, or any mixture thereof), and/or detergent adjunct ingredients such as surfactants, builders, chelators or chelating agents, bleach system or bleach components, polymers (as set forth herein), fabric conditioners, foam boosters, suds suppressors, dyes, perfume, tannish inhibitors, optical brighteners, bactericides, fungicides, soil suspending agents, anti-corrosion agents, enzyme inhibitors or stabilizers, enzyme activators, bluing agents and fluorescent dyes, antioxidants, and solubilizers. The terms “detergent” and “detergent composition” may be used interchangeably.
Detergent load is the amount of detergent used in a wash cycle.
Enzyme detergency benefit: The term “enzyme detergency benefit” is defined herein as the advantageous effect an enzyme may add to a detergent compared to the same detergent without the enzyme. Important detergency benefits which can be provided by enzymes are stain removal with no or very little visible soils after washing and/or cleaning, prevention or reduction of redeposition of soils released in the washing process (an effect that also is termed antiredeposition), restoring fully or partly the whiteness of textiles which originally were white but after repeated use and wash have obtained a greyish or yellowish appearance (an effect that also is termed whitening). Also included is the maintenance of whiteness, e.g., the prevention of greying or dullness. Textile care benefits, which are not directly related to catalytic stain removal or prevention of redeposition of soils, are also important for enzyme detergency benefits. Examples of such textile care benefits are prevention or reduction of dye transfer from one fabric to another fabric or another part of the same fabric (an effect that is also termed dye transfer inhibition or anti-backstaining), removal of protruding or broken fibers from a fabric surface to decrease pilling tendencies or remove already existing pills or fuzz (an effect that also is termed anti-pilling), improvement of the fabric-softness, colour clarification of the fabric and removal of particulate soils which are trapped in the fibers of the fabric or garment. Enzymatic bleaching is a further enzyme detergency benefit where the catalytic activity generally is used to catalyze the formation of bleaching components such as hydrogen peroxide or other peroxides.
Fabric care: The term fabric care, also referred to as textile care, refers to treatments that retains or partly or fully restores the properties of the textile, e.g. by color clarification, antipilling or prevention of formation of pills on the textile surface. Fragment: The term “fragment” means a polypeptide having one or more (e.g., several) amino acids absent from the amino and/or carboxyl terminus of a mature polypeptide or domain; wherein the fragment has protease activity.
Fungal: In the context of the present invention the term “fungal” in relation to polypeptide (such as an enzyme) refers to a polypeptide encoded by and thus directly derivable from the genome of a fungus, where such fungus has not been genetically modified to encode said polypeptide, e.g. by introducing the encoding seguence in the genome by recombinant DNA technology. In the context of the present invention, the term “fungal enzyme” or “polypeptide having enzyme activity obtained from a fungal source” thus refers to a enzyme encoded by and thus directly derivable from the genome of a fungal species, where the fungal species has not been subjected to a genetic modification introducing recombinant DNA encoding said enzyme. Thus, the nucleotide seguence encoding the fungal polypeptide having enzyme activity is a seguence naturally in the genetic background of a fungal species. The fungal polypeptide having enzyme activity encoding by such seguence may also be referred to a wildtype enzyme (or parent enzyme). In a further aspect, the invention provides polypeptides having enzyme activity, wherein said polypeptides are substantially homologous to a fungal enzyme. In the context of the present invention, the term “substantially homologous” denotes a polypeptide having enzyme activity which is at least 80%, preferably at least 85%, more preferably at least 90%, more preferably at least 95%, even more preferably at least 96%, 97%, 98%, and most preferably at least 99% identical to the amino acid seguence of a selected fungal enzyme. The polypeptides being substantially homologous to a fungal enzyme may be included in the detergent of the present invention and/or be used in the methods of the present invention.
Host cell: The term "host cell" means any cell type that is susceptible to transformation, transfection, transduction, or the like with a nucleic acid construct or expression vector comprising a polynucleotide of the present invention. The term “host cell” encompasses any progeny of a parent cell that is not identical to the parent cell due to mutations that occur during replication.
Improved stability: The term “improved stability” means an enzyme having better stability in the presence of protease relative to the stability of a reference enzyme/parent enzyme, and includes, for example, proteolytic stability, in-detergent storage stability, improved stability during production of the detergent composition, as well as in-wash stability and during rinse. Improved stability may also include stability in enzyme blends, e.g. co-granulates or liguid enzyme formulations with protease.
Improved wash performance: The term “improved wash performance” is defined herein as an enzyme displaying an increased wash performance in a detergent composition relative to the wash performance of same detergent composition without the enzyme e.g. by increased stain removal, increased color clarification and/or anti-pilling effect, when evaluating the fresh samples and/or after the samples have been stored under the same conditions. The term “improved wash performance” includes wash performance in laundry but also in, e.g., hard surface cleaning such as automated dish wash (ADW)
Isolated: The term “isolated” means a substance in a form or environment that does not occur in nature. Non-limiting examples of isolated substances include (1) any non-naturally occurring substance, (2) any substance including, but not limited to, any enzyme, variant, nucleic acid, protein, peptide or cofactor, that is at least partially removed from one or more or all of the naturally occurring constituents with which it is associated in nature; (3) any substance modified by the hand of man relative to that substance found in nature; or (4) any substance modified by increasing the amount of the substance relative to other components with which it is naturally associated (e.g., recombinant production in a host cell; multiple copies of a gene encoding the substance; and use of a stronger promoter than the promoter naturally associated with the gene encoding the substance). An isolated substance may be present in a fermentation broth sample; e.g. a host cell may be genetically modified to express the polypeptide of the invention. The fermentation broth from that host cell will comprise the isolated polypeptide.
Laundering: The term “laundering” relates to both household laundering and industrial laundering and means the process of treating textiles with a solution containing a detergent composition and optionally one or more enzymes. The laundering process can for example be carried out using e.g. a household or an industrial washing machine or can be carried out by hand.
Mature polypeptide: The term “mature polypeptide” means a polypeptide in its final form following translation and any post-translational modifications, such as N-terminal processing, C-terminal truncation, glycosylation, phosphorylation, etc.
Sequence identity: The relatedness between two amino acid sequences or between two nucleotide sequences is described by the parameter “sequence identity”. For purposes of the present invention, the sequence identity between two amino acid sequences is determined using the Needleman-Wunsch algorithm (Needleman and Wunsch, 1970, J. Mol. Biol. 48: 443-453) as implemented in the Needle program of the EMBOSS package (EMBOSS: The European Molecular Biology Open Software Suite, Rice et al., 2000, Trends Genet. 16: 276-277), preferably version 5.0.0 or later. The parameters used are gap open penalty of 10, gap extension penalty of 0.5, and the EBLOSUM62 (EMBOSS version of BLOSUM62) substitution matrix. The output of Needle labeled “longest identity” (obtained using the -nobrief option) is used as the percent identity and is calculated as follows:
(Identical Residues x 100)/(Length of Alignment - Total Number of Gaps in Alignment) For purposes of the present invention, the sequence identity between two deoxyribonucleotide sequences is determined using the Needleman-Wunsch algorithm (Needleman and Wunsch, 1970, supra) as implemented in the Needle program of the EMBOSS package (EM-BOSS: The European Molecular Biology Open Software Suite, Rice et al., 2000, supra), prefer-ably version 5.0.0 or later. The parameters used are gap open penalty of 10, gap extension penalty of 0.5, and the EDNAFULL (EMBOSS version of NCBI NLIC4.4) substitution matrix. The output of Needle labeled “longest identity” (obtained using the -nobrief option) is used as the percent identity and is calculated as follows:
(Identical Deoxyribonucleotides x 100)/(Length of Alignment - Total Number of Gaps in Alignment).
Textile: The term “textile” means any textile material including yarns, yarn intermediates, fibers, non-woven materials, natural materials, synthetic materials, and any other textile material, fabrics made of these materials and products made from fabrics (e.g., garments and other articles). The textile or fabric may be in the form of knits, wovens, denims, non-wovens, felts, yarns, and toweling. The textile may be cellulose based such as natural cellulosics, including cotton, flax/linen, jute, ramie, sisal or coir or manmade cellulosics (e.g. originating from wood pulp) including viscose/rayon, cellulose acetate fibers (tricell), lyocell or blends thereof. The textile or fabric may also be non-cellulose based such as natural polyamides including wool, camel, cashmere, mohair, rabbit and silk or synthetic polymers such as nylon, aramid, polyester, acrylic, polypropylene and spandex/elastane, or blends thereof as well as blends of cellulose based and non-cellulose based fibers. Examples of blends are blends of cotton and/or rayon/viscose with one or more companion material such as wool, synthetic fiber (e.g. polyamide fiber, acrylic fiber, polyester fiber, polyvinyl chloride fiber, polyurethane fiber, polyurea fiber, aramid fiber), and/or cellulose-containing fiber (e.g. rayon/viscose, ramie, flax/linen, jute, cellulose acetate fiber, lyocell). Fabric may be conventional washable laundry, for example stained household laundry. When the term fabric or garment is used it is intended to include the broader term textiles as well. In the context of the present invention, the term “textile” also covers fabrics. In the context of the present invention, the term “textile” is used interchangeably with fabric and cloth.
Variant: The term “variant” means a polypeptide having same activity as the parent enzyme comprising an alteration, i.e., a substitution, insertion, and/or deletion, at one or more (e.g., several) positions. A substitution means replacement of the amino acid occupying a position with a different amino acid; a deletion means removal of the amino acid occupying a position; and an insertion means adding an amino acid adjacent to and immediately following the amino acid occupying a position. In the context of the present invention, a variant of an identified enzyme has the same enzymatic activity as the parent. A variant may have least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity or at least 99% sequence identity to the parent enzyme.
Wash cycle: The term “wash cycle” is defined herein as a washing operation wherein textiles are immersed in the wash liquor, mechanical action of some kind is applied to the textile in order to release stains and to facilitate flow of wash liquor in and out of the textile and finally the superfluous wash liquor is removed. After one or more wash cycles, the textile is generally rinsed and dried.
Wash liquor: The term “wash liquor” is defined herein as the solution or mixture of water and detergent components optionally including one or more enzymes.
Wash performance: The term “wash performance” is used as detergent composition’s, enzyme’s or polymer’s capability to remove stains present on the object to be cleaned or maintain color and whiteness of textile during wash. The improvement in the wash performance may be quantified by calculating the so-called delta REM as described in Experimental section.
Weight percentage is abbreviated w/w%, wt% or w%. The abbreviations are used interchangeably.
Whiteness: The term “Whiteness” is defined herein as a broad term with different meanings in different regions and for different consumers. Whiteness can be on white textiles or be used interchangely as brightness for colored textiles. Loss of whiteness or brightness can e.g. be due to greying, yellowing, or removal of optical brighteners/hueing agents. Greying and yellowing can be due to soil redeposition, stain redeposition, dirt/mud redeposition, pollution particles, body soils, colouring from e.g. iron and copper ions or dye transfer. Loss of whiteness might include one or several issues from the list below: colourant or dye effects; incomplete stain removal (e.g. body soils, sebum etc.); redeposition (greying, yellowing or other discolourations of the object) (removed soils reassociate with other parts of textile, soiled or unsoiled); chemical changes in textile during application; and clarification or brightening of colours. SEQUENCE OVERVIEW
SEQ ID NO:1 is a protease
SEQ ID NO:2 is a protease
SEQ ID NO:3 is a protease
SEQ ID NO:4 is a lipase
SEQ ID NO:5 is an amylase
SEQ ID NO:6 is a mannanase
SEQ ID NO:7 is a mannanase
SEQ ID NO:8 is a xyloglucanase
SEQ ID NO:9 is a cellulase
SEQ ID NO: 10 is a pectinase
SEQ ID NO:11 is a DNase
SEQ ID NO:12 is a cellulase
SEQ ID NO: 13 is a protease
SEQ ID NO: 14 is a protease
SEQ ID NO: 15 is a protease
DETAILED DESCRIPTION OF THE INVENTION
Compaction of the detergents, improvement of performance, such as anti-redeposition, and the desire to replace detergent ingredients with active ingredients that are more sustainable calls for use of new enzyme combinations that are even more effective than the ones on the market today, both in terms of stability of the enzymes, the ability to replace non-biological detergent ingredients with enzymes and good performance at lower wash temperature, improved stain removal and/or whiteness, and/or shorter wash cycles than traditionally used today. The inventors of the present invention have identified several new enzyme compositions that are useful for the above purposes.
Accordingly, the present invention concerns a detergent composition comprising at least one surfactant a mannanase or a combination of mannanases and a further enzyme selected from the group consisting of a. a xyloglucanase having at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8 b. a cellulase having at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 9, and c. a cellulase having at least 80% sequence identity to SEQ ID NO: 12, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 12.
In a further embodiment, the invention concerns a detergent composition comprising at least one surfactant, a mannanase selected from the group consisting of a. a mannanase having at least 80% sequence identity to SEQ ID NO: 7, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity SEQ ID NO: 7, b. a mannanase having at least 80% sequence identity to SEQ ID NO: 6 at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity SEQ ID NO: 6, and c. a blend of a mannanase having at least 80% sequence identity to SEQ ID NO: 7, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity SEQ ID NO: 7, and a mannanase having at least 80% sequence identity to SEQ ID NO: 6, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity SEQ ID NO: 6, and a further enzyme selected from the group consisting of a. a xyloglucanase having at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8 b. a cellulase having at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 9, and c. a cellulase having at least 80% sequence identity to SEQ ID NO: 12, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 12.
In a particular embodiment the invention concerns a detergent composition, wherein said composition comprises a mannanase having at least 80% sequence identity to SEQ ID NO: 7, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity SEQ ID NO: 7, and a further enzyme selected form the group consisting of a. a xyloglucanase having at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8 b. a cellulase having at least 80% sequence identity to SEQ ID NO: 12, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 12.
In a further particular embodiment invention concerns the use the detergent composition of any of the above embodiments above for the improvement of prevention of redeposition during laundering.
In another embodiment, the invention concerns an enzyme compositions of the present invention comprises at least two, such as two, three, four, five, six, seven, eight or nine enzymes selected from the group of enzymes having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 1 ; at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 2; at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 3; at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 4; at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5; at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 6; at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7; at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8; at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 9; at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10; at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 11 ; at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 12; at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 13; and at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 14.
In a preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14 and a lipase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 4.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, and an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5. In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, and a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2 , SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, and a cellulase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 9.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2 , SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, and a DNase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 11.
In another preferred embodiment the enzyme composition comprises a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8, and a cellulase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 9.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2 , SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5 and a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2 , SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5 and a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2 , SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 6, and a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2 , SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 6, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7, a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8, and pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2 , SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, a lipase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 4, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 6, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7, and a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2 , SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, a lipase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 4, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 6, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7, and a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2 , SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, a lipase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 4, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 6, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7, a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8, a cellulase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 9, and a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2 , SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, a lipase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 4, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 6, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7, a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8, a cellulase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 9, a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least
91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least
94% sequence identity at least 95% sequence identity at least 96% sequence identity at least
97% sequence identity at least 98% sequence identity at least 99% sequence identity or even
100% sequence identity to SEQ ID NO: 10, and a DNase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 11.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2 , SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 6, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7, a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8, a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10, and a DNase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 11.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2 , SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 6, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7, a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8, a cellulase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 9, a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10, and a DNase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 11.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2 , SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, a lipase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 4, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 6, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7, a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8, a cellulase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 9, a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10, and a DNase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 11 .
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2 , SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, a lipase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 4, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 6, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7, a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8, a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10, and a DNase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 11.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2 , SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, and a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 6.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2 , SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 6, a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8, and pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2 , SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, a lipase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 4, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 6, and a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, a lipase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 4, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 6, and a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, a lipase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 4, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 6, a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8, a cellulase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 9, and a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, a lipase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 4, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 6, a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8, a cellulase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 9, a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10, and a DNase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 11.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 6, a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8, a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10, and a DNase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 11.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 6, a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8, a cellulase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 9, a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10, and a DNase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 11 .
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, a lipase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 4, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 6, a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8, a cellulase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 9, a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10, and a DNase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 11.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, a lipase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 4, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 6, a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8, a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10, and a DNase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 11.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, and a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7, a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8, and pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, a lipase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 4, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7, and a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, a lipase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 4, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7, and a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, a lipase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 4, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7, a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8, a cellulase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 9, and a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, a lipase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 4, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7, a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8, a cellulase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 9, a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10, and a DNase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 11.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7, a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8, a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10, and a DNase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 11.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7, a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8, a cellulase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 9, a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10, and a DNase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 11 .
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, a lipase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 4, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7, a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8, a cellulase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 9, a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10, and a DNase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 11.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 13 or SEQ ID NO: 14, a lipase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 4, an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 5, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7, a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 8, a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10, and a DNase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 11.
In another preferred embodiment the enzyme composition comprises a cellulase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:9 or SEQ ID NO: 12 and a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:8.
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO:1, or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:6 or SEQ ID NO: 7, a lipase at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to to SEQ ID NO:4, and an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:5;
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a lipase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least
91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least
94% sequence identity at least 95% sequence identity at least 96% sequence identity at least
97% sequence identity at least 98% sequence identity at least 99% sequence identity or even
100% sequence identity to SEQ ID NO:4, and an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:5; and In another preferred embodiment the enzyme composition comprises a protease having at least 80% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO: 14, a lipase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:4, a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:8, and an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:5.
In another preferred embodiment the enzyme composition comprises a cellulase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:9 and a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:8;
In another preferred embodiment the enzyme composition comprises a protease having at least 80% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO: 14, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:6, a mannanase at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7, and an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:5;
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO:1, or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:6, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7, a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10, a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:8 and an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:5;
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:6, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7, a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10, a lipase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:4 and an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:5;
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:6, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7, a lipase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:4, a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:8 and an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:5;
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:6, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7, a lipase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:4, a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10, a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:8, a cellulase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:9, and an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least
91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least
94% sequence identity at least 95% sequence identity at least 96% sequence identity at least
97% sequence identity at least 98% sequence identity at least 99% sequence identity or even
100% sequence identity to SEQ ID NO:5;
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:6, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7, a lipase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least
91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least
94% sequence identity at least 95% sequence identity at least 96% sequence identity at least
97% sequence identity at least 98% sequence identity at least 99% sequence identity or even
100% sequence identity to SEQ ID NO:4, a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10, a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:8, a cellulase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:9, a DNase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:11 , and an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91% sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:5;
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:6, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7, a lipase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:4, a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 10, a xyloglucanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:8, a DNase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:11 , and an amylase having at least 80% sequence identity to SEQ ID NO:5;
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:6, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7, a pectinase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:9, a DNase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:11 , and an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:5;
In another preferred embodiment the enzyme composition comprises a protease having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:6, a mannanase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO: 7, a lipase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least
91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least
94% sequence identity at least 95% sequence identity at least 96% sequence identity at least
97% sequence identity at least 98% sequence identity at least 99% sequence identity or even
100% sequence identity to SEQ ID NO:4, and an amylase having at least 60% sequence identity, such as at least 70% sequence identity, at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 91 % sequence identity, at least 92% sequence identity, at least 93% sequence identity at least 94% sequence identity at least 95% sequence identity at least 96% sequence identity at least 97% sequence identity at least 98% sequence identity at least 99% sequence identity or even 100% sequence identity to SEQ ID NO:5.
In an embodiment, the enzymes of the present invention may comprise one or more conservative amino acid substitutions that do not alter their physico-chemical properties, such as thermal stability of the polypeptide, alter the substrate specificity, change the pH optimumsignificantly. Examples of conservative substitutions are within the groups of basic amino acids (arginine, lysine and histidine), acidic amino acids (glutamic acid and aspartic acid), polar amino acids (glutamine and asparagine), hydrophobic amino acids (leucine, isoleucine and valine), aromatic amino acids (phenylalanine, tryptophan and tyrosine), and small amino acids (glycine, alanine, serine, threonine and methionine). Amino acid substitutions that do not generally alter specific activity are known in the art and are described, for example, by H. Neurath and R.L. Hill, 1979, In, The Proteins, Academic Press, New York. Common substitutions are Ala/Ser, Val/lle, Asp/Glu, Thr/Ser, Ala/Gly, Ala/Thr, Ser/Asn, Ala/Val, Ser/Gly, Tyr/Phe, Ala/Pro, Lys/Arg, Asp/Asn, Leu/lle, Leu/Val, Ala/Glu, and Asp/Gly.
The enzyme composition may be in a solid form, such as a granulate composition, or in a liquid form. When the enzyme composition is in a liquid form, the enzymes may be stabilized using conventional stabilizing agents, e.g. a polyol such as propylene glycol or glycerol, a sugar or sugar alcohol, lactic acid, boric acid, or a boric acid derivative, e.g. an aromatic borate ester, or a phenyl boronic acid derivative such as 4-formylphenyl boronic acid, and the composition may be formulated as described in, for example, WO92/19709 and WO92/19708.
The enzyme composition of the present invention may form part of a detergent composition comprising at least one surfactant. Said detergent composition comprises in a preferred embodiment comprising 0.05-20 wt% rhamnolipid, such as 1-15 wt% rhamnolipid, such as 2-10 wt% rhamnolipid, such as 3-9 wt% rhamnolipid, such as 4-8 wt% rhamnolipid. Preferably at least 70 wt%, such as 85 wt%, 90 wt% or 95 wt% of the rhamnolipid is mono rhamnolipid.
In particular the detergent composition is useful for laundry, textile cleaning or hard surface cleaning. When the enzyme composition is applied in a wash cycle, the total concentration of the enzymes in the wash liquor is typically from about 0.2 ppm to 20 ppm in solution. The water temperatures in a wash cycle typically range from 5°C to 90°C, or in the range of 10°C to 80°C, or in the range of 10°C to 70°C, or in the range of 10°C to 60°C, or in the range of 10°C to 50°C, or in the range of 15°C to 40°C, or in the range of 20°C to 30°C. The water to fabric ratio is typically from about 1 :1 to about 30:1. Accordingly, the present invention relates to a method for removal of a stain on a textile during a wash cycle comprising contacting the textile with a detergent composition comprising the enzyme composition of the invention, completing the wash cycle at least one time at the above indicated temperture, optionally followed by rinsing the textile.
Liquid enzyme formulations
The enzymes of the enzyme composition may be formulated as a liquid enzyme formulation, which is generally a pourable composition, though it may also have a high viscosity. The physical appearance and properties of a liquid enzyme formulation may vary a lot - for example, they may have different viscosities (gel to water-like), be colored, not colored, clear, hazy, and even with solid particles like in slurries and suspensions. The minimum ingredients are the enzymes (protease, lipase and other enzymes present) and a solvent system to make it a liquid.
The solvent system may comprise water, polyols (such as glycerol, (mono, di, or tri) propylene glycol, (mono, di, or tri) ethylene glycol, sugar alcohol (e.g. sorbitol, mannitol, erythritol, dulcitol, inositol, xylitol or adonitol), polypropylene glycol, and/or polyethylene glycol), ethanol, sugars, sugar alcohols, sorbitol, mannitol, fructose, sucrose, trehalose, erythritol, dulcitol, inositol, xylitol, adonitol, lactic acid as well as various polymers (e.g. PVP). Additionally, salts may be added to increase the ionic strength. Usually, the solvent system also includes a preservation agent and/or other stabilizing agents such as ethylenediaminetetraacetic acid (EDTA), boric acid, or a boric acid derivative, e.g. an aromatic borate ester, or a phenyl boronic acid derivative such as 4-formylphenyl boronic acid, and the composition may be formulated as described in, for example, WO92/19709 and WO92/19708.
The optimal pH depends on the specific enzyme but is typically in the range of pH 4-9. In some cases, surfactants like nonionic surfactant (e.g., alcohol ethoxylates) can improve the physical stability of the enzyme formulations.
In one embodiment the enzyme composition comprises the enzymes and further:
(i) a polyol, preferably selected from glycerol, (mono, di, or tri) propylene glycol, (mono, di, or tri) ethylene glycol, polyethylene glycol, sugar alcohols, sorbitol, mannitol, erythritol, dulcitol, inositol, xylitol and adonitol;
(ii) optionally a surfactant, preferably selected from anionic and nonionic surfactants, (iii) optionally a salt, divalent cation (such as Mg++ or Ca++), polymer, or enzyme inhibitor;
(iv) optionally having a pH in the range of pH 4-9; and
(v) water.
Slurries or dispersions of enzymes are typically prepared by dispersing small particles of enzymes (e.g., spray-dried particles) in a liquid medium in which the enzyme is sparingly soluble, e.g., a liquid nonionic surfactant or a liquid polyethylene glycol. Powder can also be added to aqueous systems in an amount so not all go into solution (above the solubility limit). Another format is crystal suspensions which can also be aqueous liquids (see for example WO2019/002356). Another way to prepare such dispersion is by preparing water-in-oil emulsions, where the enzyme is in the water phase, and evaporate the water from the droplets. Such slurries/suspension can be physically stabilized (to reduce or avoid sedimentation) by addition of rheology modifiers, such as fumed silica or xanthan gum, typically to get a shear thinning rheology.
Granular enzyme formulations
The enzymes comprised in the enzyme composition of the present invention may also be formulated as a solid/granular enzyme formulation. Non-dusting granulates may be produced, e.g. as disclosed in US 4,106,991 and US 4,661 ,452, and may optionally be coated by methods known in the art. Examples of waxy coating materials are poly(ethylene oxide) products (polyethyleneglycol, PEG) with mean molar weights of 1000 to 20000; ethoxylated nonylphenols having from 16 to 50 ethylene oxide units; ethoxylated fatty alcohols in which the alcohol contains from 12 to 20 carbon atoms and in which there are 15 to 80 ethylene oxide units; fatty alcohols; fatty acids; and mono- and di- and triglycerides of fatty acids. Examples of film-forming coating materials suitable for application by fluid bed techniques are given in GB 1483591.
Proteases may be formulated as a granule for example as a co-granule that combines one or more enzymes or benefit agents (such as MnTACN or other bleaching components). Each enzyme will then be present in more granules securing a more uniform distribution of enzymes in the detergent. This also reduces the physical segregation of different enzymes due to different particle sizes. Methods for producing multi-enzyme co-granulate for the detergent industry are disclosed in the IP.com disclosure IPCGM000200739D.
An embodiment of the invention relates to an enzyme granule/particle comprising proteases. The granule is composed of a core, and optionally one or more coatings (outer layers) surrounding the core. Typically, the granule/particle size, measured as equivalent spherical diameter (volume based average particle size), of the granule is 20-2000 pm, particularly 50-1500 pm, 100-1500 pm or 250-1200 pm.
The core may include additional materials such as fillers, fibre materials (cellulose or synthetic fibers), stabilizing agents, solubilising agents, suspension agents, viscosity regulating agents, light spheres, plasticizers, salts, lubricants and fragrances. The core may include binders, such as synthetic polymer, wax, fat, or carbohydrate. The core may comprise a salt of a multivalent cation, a reducing agent, an antioxidant, a peroxide decomposing catalyst and/or an acidic buffer component, typically as a homogenous blend. The core may consist of an inert particle with the enzyme absorbed into it, or applied onto the surface, e.g., by fluid bed coating. The core may have a diameter of 20-2000 pm, particularly 50-1500 pm, 100-1500 pm or 250- 1200 pm. The core can be prepared by granulating a blend of the ingredients, e.g., by a method comprising granulation techniques such as crystallization, precipitation, pan-coating, fluid bed coating, fluid bed agglomeration, rotary atomization, extrusion, prilling, spheronization, size reduction methods, drum granulation, and/or high shear granulation. Methods for preparing the core can be found in Handbook of Powder Technology; Particle size enlargement by C. E. Capes; Volume 1 ; 1980; Elsevier. These methods are well-known in the art and have also been described in international patent application WO2015/028567, pages 3-5, which is incorporated by reference.
The core of the enzyme granule/particle may be surrounded by at least one coating, e.g., to improve the storage stability, to reduce dust formation during handling, or for coloring the granule. The optional coating(s) may include a salt coating, or other suitable coating materials, such as polyethylene glycol (PEG), methyl hydroxy-propyl cellulose (MHPC) and polyvinyl alcohol (PVA). Examples of enzyme granules with multiple coatings are shown in WO 93/07263 and WO 97/23606.
Such coatings are well-known in the art, and have earlier been described in, for example, WO00/01793, W02001/025412, and WO2015/028567, which are incorporated by reference.
In one aspect, the present invention provides a granule, which comprises:
(a) a core comprising a protease according to the invention; and
(b) optionally a (salt) coating consisting of one or more layer(s) surrounding the core.
Another aspect of the invention relates to a layered granule, comprising:
(a) a (non-enzymatic) core;
(b) a coating surrounding the core, wherein the coating comprises a protease; and (c) optionally a (salt) coating consisting of one or more layer(s) surrounding the enzyme containing coating.
Encapsulated enzyme formulations
The enzymes may also be formulated as an encapsulated enzyme formulation (an ‘encapsulate’). This is particularly useful for separating the enzyme from other ingredients when the enzyme is added into, for example, a (liquid) cleaning composition, such as the detergent compositions described below.
Physical separation can be used to solve incompatibility between the enzyme(s) and other components. Incompatibility can arise if the other components are either reactive against the enzyme, or if the other components are substrates of the enzyme. Other enzymes can be substrates of proteases.
The enzyme may be encapsulated in a matrix, preferably a water-soluble or water dispersible matrix (e.g., water-soluble polymer particles), for example as described in WO 2016/023685. An example of a water-soluble polymeric matrix is a matrix composition comprising polyvinyl alcohol. Such compositions are also used for encapsulating detergent compositions in unit-dose formats.
The enzyme may also be encapsulated in core-shell microcapsules, for example as described in WO 2015/144784, or as described in the IP.com disclosure IPCOM000239419D.
Such core-shell capsules can be prepared using a number of technologies known in the art, e.g., by interfacial polymerization using either a water-in-oil or an oil-in-water emulsion, where polymers are crosslinked at the surface of the droplets in the emulsion (the interface between water and oil), thus forming a wall/membrane around each droplet/capsule.
Formulation of enzyme in co-granule
The enzymes may be formulated as a granule for example as a co-granule that combines one or more enzymes. Each enzyme will then be present in more granules securing a more uniform distribution of enzymes in the detergent. This also reduces the physical segregation of different enzymes due to different particle sizes. Methods for producing multi-enzyme co-granulates for the detergent industry are disclosed in the IP.com disclosure IPCOM000200739D.
Another example of formulation of enzymes by the use of co-granulates are disclosed in WO 2013/188331 , which relates to a detergent composition comprising (a) a multi-enzyme co- granule; (b) less than 10 wt% zeolite (anhydrous basis); and (c) less than 10 wt% phosphate salt (anhydrous basis), wherein said enzyme co-granule comprises from 10 wt% to 98 wt% moisture sink component and the composition additionally comprises from 20 wt% to 80 wt% detergent moisture sink component.
WO 2013/188331 also relates to a method of treating and/or cleaning a surface, preferably a fabric surface comprising the steps of (i) contacting said surface with the detergent composition as claimed and described herein in an aqueous wash liquor, (ii) rinsing and/or drying the surface.
The multi-enzyme co-granule may comprise a proteases and (a) one or more enzymes selected from the group consisting of lipases, xyloglucanases, perhydrolases, peroxidases, proteases, laccases and mixtures thereof; and (b) one or more enzymes selected from the group consisting of hemicellulases, care cellulases, cellulases, cellobiose dehydrogenases, xylanases, phospho lipases, esterases, cutinases, pectinases, mannanases, pectate lyases, keratinases, reductases, oxidases, phenoloxidases, ligninases, pullulanases, tannases, pentosanases, lichenases glucanases, arabinosidases, hyaluronidase, chondroitinase, amylases, DNAse, and mixtures thereof.
Purity of enzyme in formulations
The enzymes used in the enzyme compositions of the present invention may be purified to any desired degree of purity. This includes high levels of purification, as achieved for example by using methods of crystallization - but also none or low levels of purification, as achieved for example by using crude fermentation broth, as described in W02001/025411 , or in WO2009/152176.
Microorganisms
The enzyme formulations, as well as the detergent formulations described below, may comprise one or more microorganisms or microbes. Generally, any microorganism(s) may be used in the enzyme/detergent formulations in any suitable amount(s)/concentration(s). Microorganisms may be used as the only biologically active ingredient, but they may also be used in conjunction with one or more of the enzymes described above.
The purpose of adding the microorganism(s) may, for example, be to reduce malodor as described in WO 2012/112718. Other purposes could include in-situ production of desirable biological compounds, or inoculation/population of a locus with the microorganism(s) to competitively prevent other non-desirable microorganisms form populating the same locus (competitive exclusion).
The term “microorganism” generally means small organisms that are visible through a microscope. Microorganisms often exist as single cells or as colonies of cells. Some microorganisms may be multicellular. Microorganisms include prokaryotic (e.g., bacteria and archaea) and eukaryotic (e.g., some fungi, algae, protozoa) organisms. Examples of bacteria may be Gram-positive bacteria or Gram-negative bacteria. Example forms of bacteria include vegetative cells and endospores. Examples of fungi may be yeasts, molds and mushrooms. Example forms of fungi include hyphae and spores. Herein, viruses may be considered microorganisms.
Microorganisms may be recombinant or non-recombinant. In some examples, the microorganisms may produce various substances (e.g., enzymes) that are useful for inclusion in detergent compositions. Extracts from microorganisms or fractions from the extracts may be used in the detergents. Media in which microorganisms are cultivated or extracts or fractions from the media may also be used in detergents. In some examples, specific of the microorganisms, substances produced by the microorganisms, extracts, media, and fractions thereof, may be specifically excluded from the detergents. In some examples, the microorganisms, or substances produced by, or extracted from, the microorganisms, may activate, enhance, preserve, prolong, and the like, detergent activity or components contained with detergents.
Generally, microorganisms may be cultivated using methods known in the art. The microorganisms may then be processed or formulated in various ways. In some examples, the microorganisms may be desiccated (e.g., lyophilized). In some examples, the microorganisms may be encapsulated (e.g., spray drying). Many other treatments or formulations are possible. These treatments or preparations may facilitate retention of microorganism viability over time and/or in the presence of detergent components. In some examples, however, microorganisms in detergents may not be viable. The processed/formulated microorganisms may be added to detergents prior to, or at the time the detergents are used.
In one embodiment, the microorganism is a species of Bacillus, for example, at least one species of Bacillus selected from the group consisting of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus atrophaeus, Bacillus pumilus, Bacillus megaterium, or a combination thereof. In a preferred embodiment, the aforementioned Bacillus species are on an endospore form, which significantly improves the storage stability.
Detergent compositions
In one embodiment, the invention is directed to detergent compositions comprising the enzyme composition in combination with one or more additional cleaning composition components. In one embodiment, the detergent composition comprises at least two polypeptides with an amino acid sequence having at least 60% identity, such as 70%, 80%, 90%, 95%, 96%, 97%, 98%, 99% or even 100% identity to the amino acid sequence set forth in SEQ ID NO:1 to SEQ ID NO: 14. In one embodiment the detergent composition is in solid form. In another embodiment, the detergent composition is in a liquid or gel form. In another embodiment a bar form. In one embodiment the detergent may be wrapped in water soluble PVOH film. The choice of additional components is within the skill of the artisan and includes conventional ingredients, including the exemplary nonlimiting components set forth below.
Liquid detergent composition
The liquid detergent composition may comprise a microcapsule, and thus form part of any detergent composition in any form, such as liquid and powder detergents, and soap and detergent bars.
In one embodiment, the invention is directed to liquid detergent compositions comprising a microcapsule, as described above, in combination with one or more additional cleaning composition components.
The microcapsule, as described above, may be added to the liquid detergent composition in an amount corresponding to from 0.1% to 5% (w/w) active enzyme protein; preferably from 0. 1% to 4%, such as from 0.1% to 3%, such as from 0.1% to 2.5%, such as from 0.1% to 2%, such as from 0.1% to 1.5%, such as from 0.1% to 1%, and most preferably from 0.01% to 1% (w/w) active enzyme protein.
The liquid detergent composition has a physical form, which is not solid (or gas). It may be a pourable liquid, a paste, a pourable gel or a non-pourable gel. It may be either isotropic or structured, preferably isotropic. It may be a formulation useful for washing in automatic washing machines or for hand washing. It may also be a personal care product, such as a shampoo, toothpaste, or a hand soap.
The liquid detergent composition may be aqueous, typically containing at least 20% by weight and up to 95% water, such as up to 70% water, up to 50% water, up to 40% water, up to 30% water, or up to 20% water. Other types of liquids, including without limitation, alkanols, amines, diols, ethers and polyols may be included in an aqueous liquid detergent. An aqueous liquid detergent may contain from 0-30% organic solvent. A liquid detergent may even be non-aqueous, wherein the water content is below 10%, preferably below 5%.
Detergent ingredients can be separated physically from each other by compartments in water dissolvable pouches. Thereby negative storage interaction between components can be avoided. Different dissolution profiles of each of the compartments can also give rise to delayed dissolution of selected components in the wash solution.
The detergent composition may take the form of a unit dose product. A unit dose product is the packaging of a single dose in a non-reusable container. It is increasingly used in detergents for laundry. A detergent unit dose product is the packaging (e.g., in a pouch made from a water-soluble film) of the amount of detergent used for a single wash.
Pouches can be of any form, shape and material which is suitable for holding the composition, e.g., without allowing the release of the composition from the pouch prior to water contact. The pouch is made from water soluble film which encloses an inner volume. Said inner volume can be divided into compartments of the pouch. Preferred films are polymeric materials preferably polymers which are formed into a film or sheet. Preferred polymers, copolymers or derivates thereof are selected polyacrylates, and water-soluble acrylate copolymers, methyl cellulose, carboxy methyl cellulose, sodium dextrin, ethyl cellulose, hydroxyethyl cellulose, hydroxypropyl methyl cellulose, maltodextrin, polymethacrylates, most preferably polyvinyl alcohol copolymers and, hydroxypropyl methyl cellulose (HPMC). Preferably the level of polymer in the film for example PVA is at least about 60%. Preferred average molecular weight will typically be about 20,000 to about 150,000. Films can also be a blend composition comprising hydrolytically degradable and water-soluble polymer blends such as polyactide and polyvinyl alcohol (known under the Trade reference M8630 as sold by Chris Craft In. Prod. Of Gary, Ind., US) plus plasticizers like glycerol, ethylene glycerol, Propylene glycol, sorbitol and mixtures thereof. The pouches can comprise a solid laundry cleaning composition or part components and/or a liquid cleaning composition or part components separated by the water-soluble film. The compartment for liquid components can be different in composition than compartments containing solids (see e.g., US 2009/0011970).
Detergent ingredients
The choice of detergent components may include, for textile care, the consideration of the type of textile to be cleaned, the type and/or degree of soiling, the temperature at which cleaning is to take place, and the formulation of the detergent product. Although components mentioned below are categorized by general header according to a particular functionality, this is not to be construed as a limitation, as a component may comprise additional functionalities as will be appreciated by the skilled artisan.
Any detergent components known in the art for use in detergents may also be utilized. Other optional detergent components include anti-corrosion agents, anti-shrink agents, anti-soil redeposition agents, anti-wrinkling agents, bactericides, binders, corrosion inhibitors, disintegrants/disintegration agents, dyes, enzyme stabilizers (including boric acid, borates, and/or polyols such as propylene glycol), fabric conditioners including clays, fillers/processing aids, fluorescent whitening agents/optical brighteners, foam boosters, foam (suds) regulators, perfumes, soil-suspending agents, softeners, suds suppressors, tarnish inhibitors, and wicking agents, either alone or in combination. Any ingredient known in the art for use in detergents may be utilized. The choice of such ingredients is well within the skill of the artisan and includes conventional ingredients, including the exemplary non-limiting components set forth below. Surfactants
The cleaning composition may comprise one or more surfactants, which may be anionic and/or cationic and/or non-ionic and/or semi-polar and/or zwitterionic, or a mixture thereof. In a particular embodiment, the detergent composition includes a surfactant system (comprising more than one surfactant) e.g. a mixture of one or more nonionic surfactants and one or more anionic surfactants. In one embodiment the detergent comprises at least one anionic surfactant and at least one non-ionic surfactant, the weight ratio of anionic to nonionic surfactant may be from 20:1 to 1:20. In one embodiment the amount of anionic surfactant is higher than the amount of non-ionic surfactant e.g. the weight ratio of anionic to non-ionic surfactant may be from 10:1 to 1.1 : 1 or from 5:1 to 1.5: 1. The amount of anionic to non-ionic surfactant may also be equal and the weight ratios 1 :1. In one embodiment the amount of non-ionic surfactant is higher than the amount of anionic surfactant and the weight ratio may be 1 : 10 to 1 : 1.1. Preferably the weight ratio of anionic to non-ionic surfactant is from 10: 1 to 1 : 10, such as from 5: 1 to 1 :5, or from 5: 1 to 1 : 1.2. Preferably, the weight fraction of non- ionic surfactant to anionic surfactant is from 0 to 0.5 or 0 to 0.2 thus non-ionic surfactant can be present or absent if the weight fraction is 0, but if non-ionic surfactant is present, then the weight fraction of the nonionic surfactant is preferably at most 50% or at most 20% of the total weight of anionic surfactant and non-ionic surfactant. Light duty detergent usually comprises more nonionic than anionic surfactant and there the fraction of non-ionic surfactant to anionic surfactant is preferably from 0.5 to 0.9. The total weight of surfactant(s) is typically present at a level of from about 0.1% to about 60% by weight, such as about 1% to about 40%, or about 3% to about 20%, or about 3% to about 10%. The surfactant(s) is chosen based on the desired cleaning application, and may include any conventional surfactant(s) known in the art. When included therein the detergent will usually contain from about 1 % to about 40% by weight of an anionic surfactant, such as from about 5% to about 30%, including from about 5% to about 15%, or from about 15% to about 20%, or from about 20% to about 25% of an anionic surfactant. Non-limiting examples of anionic surfactants include sulfates and sulfonates, typically available as sodium or potassium salts or salts of monoethanolamine (MEA, 2-aminoethan-1-ol) or triethanolamine (TEA, 2,2',2"-nitrilotriethan-1-ol); in particular, linear alkylbenzenesulfonates (LAS), isomers of LAS such as branched alkylbenzenesulfonates (BABS) and phenylalkanesulfonates; olefin sulfonates, in particular alphaolefinsulfonates (AOS); alkyl sulfates (AS), in particular fatty alcohol sulfates (FAS), i.e., primary alcohol sulfates (PAS) such as dodecyl sulfate (SLS); alcohol ethersulfates (AES or AEOS or FES, also known as alcohol ethoxysulfates or fatty alcohol ether sulfates); paraffin sulfonates (PS) including alkane-1 -sulfonates and secondary alkanesulfonates (SAS); ester sulfonates, including sulfonated fatty acid glycerol esters and alpha-sulfo fatty acid methyl esters (alpha-SFMe or SES or MES); alkyl- or alkenylsuccinic acids such as dodecenyl/tetradecenyl succinic acid (DTSA); diesters and monoesters of sulfosuccinic acid; fatty acid derivatives of amino acids. Anionic surfactants may be added as acids, as salts or as ethanolamine derivatives.
When included therein the detergent will usually contain from about 0,1% to about 40% by weight of a cationic surfactant, for example from about 0.5% to about 30%, in particular from about 1% to about 20%, from about 3% to about 10%, such as from about 3% to about 5%, from about 8% to about 12% or from about 10% to about 12%. Non-limiting examples of cationic surfactants include alkyldimethylethanolamine quat (ADMEAQ), cetyltrimethylammonium bromide (CTAB), dimethyldistearylammonium chloride (DSDMAC), and alkylbenzyldimethylammonium, alkyl quaternary ammonium compounds, alkoxylated quaternary ammonium (AQA) compounds, ester quats, and combinations thereof.
When included therein the detergent will usually contain from about 0.2% to about 40% by weight of a nonionic surfactant, for example from about 0.5% to about 30%, in particular from about 1% to about 20%, from about 3% to about 10%, such as from about 3% to about 5%, from about 8% to about 12%, or from about 10% to about 12%. Non-limiting examples of nonionic surfactants include alcohol ethoxylates (AE or AEO) e.g. the AEO-series such as AEO-7, alcohol propoxylates, in particular propoxylated fatty alcohols (PFA), ethoxylated and propoxylated alcohols, alkoxylated fatty acid alkyl esters, such as ethoxylated and/or propoxylated fatty acid alkyl esters (in particular methyl ester ethoxylates, MEE), alkylpolyglycosides (APG), alkoxylated amines, fatty acid monoethanolamides (FAM), fatty acid diethanolamides (FADA), ethoxylated fatty acid monoethanolamides (EFAM), propoxylated fatty acid monoethanolamides (PFAM), polyhydroxyalkyl fatty acid amides, or N-acyl N-alkyl derivatives of glucosamine (glucamides, GA, or fatty acid glucamides, FAGA), as well as products available under the trade names SPAN and TWEEN, and combinations thereof.
When included therein the detergent will usually contain from about 0.01 to about 10 % by weight of a semipolar surfactant. Non-limiting examples of semipolar surfactants include amine oxides (AO) such as alkyldimethylamine oxides, in particular N-(coco alkyl)-N,N-dimethylamine oxide and N-(tallow-alkyl)-N,N-bis(2-hydroxyethyl)amine oxide, and combinations thereof.
When included therein the detergent will usually contain from about 0.01 % to about 10 % by weight of a zwitterionic surfactant. Non-limiting examples of zwitterionic surfactants include betaines such as alkyldimethylbetaines, sulfobetaines, and combinations thereof.
Additional bio-based surfactants may be used e.g. wherein the surfactant is a sugar-based non-ionic surfactant which may be a hexyl-p-D-maltopyranoside, thiomaltopyranoside or a cyclic- maltopyranoside, such as described in EP2516606 B1. Other biosurfactants may include rhamnolipids and sophorolipids. Hydrotropes
A hydrotrope is a compound that solubilises hydrophobic compounds in aqueous solutions (or oppositely, polar substances in a non-polar environment). Typically, hydrotropes have both hydrophilic and a hydrophobic character (so-called amphiphilic properties as known from surfactants); however, the molecular structure of hydrotropes generally do not favor spontaneous self-aggregation, see e.g. review by Hodgdon and Kaier (2007), Current Opinion in Colloid & Interface Science 12:121-128. Hydrotropes do not display a critical concentration above which self-aggregation occurs as found for surfactants and lipids forming miceller, lamellar or other well defined meso-phases. Instead, many hydrotropes show a continuous-type aggregation process where the sizes of aggregates grow as concentration increases. However, many hydrotropes alter the phase behavior, stability, and colloidal properties of systems containing substances of polar and non-polar character, including mixtures of water, oil, surfactants, and polymers. Hydrotropes are classically used across industries from pharma, personal care, food, to technical applications. Use of hydrotropes in detergent compositions allow for example more concentrated formulations of surfactants (as in the process of compacting liquid detergents by removing water) without inducing undesired phenomena such as phase separation or high viscosity.
The detergent may contain 0-10% by weight, for example 0-5% by weight, such as about 0.5 to about 5%, or about 3% to about 5%, of a hydrotrope. Any hydrotrope known in the art for use in detergents may be utilized. Non-limiting examples of hydrotropes include sodium benzenesulfonate, sodium p-toluene sulfonate (STS), sodium xylene sulfonate (SXS), sodium cumene sulfonate (SCS), sodium cymene sulfonate, amine oxides, alcohols and polyglycolethers, sodium hydroxynaphthoate, sodium hydroxynaphthalene sulfonate, sodium ethylhexyl sulfate, and combinations thereof.
Builders and Co-Builders
The detergent composition may contain about 0-65% by weight, such as about 5% to about 50% of a detergent builder or co-builder, or a mixture thereof. The builder and/or co-builder may particularly be a chelating agent that forms water-soluble complexes with Ca and Mg. Any builder and/or co-builder known in the art for use in cleaning detergents may be utilized.
Non-limiting examples of builders include zeolites, diphosphates (pyrophosphates), triphosphates such as sodium triphosphate (STP or STPP), carbonates such as sodium carbonate, soluble silicates such as sodium metasilicate, layered silicates (e.g., SKS-6 from Clariant), ethanolamines such as 2-aminoethan-1-ol (MEA), diethanolamine (DEA, also known as 2,2'- iminodiethan-1-ol), triethanolamine (TEA, also known as 2,2',2"-nitrilotriethan-1-ol), and (carboxymethyl)inulin (CM I), and combinations thereof. The detergent composition may also contain from about 0-50% by weight, such as about 5% to about 30%, of a detergent co-builder. The detergent composition may include a co-builder alone, or in combination with a builder, for example a zeolite builder. Non-limiting examples of co-builders include or copolymers thereof, such as poly(acrylic acid) (PAA) or copoly(acrylic acid/maleic acid) (PAA/PMA). According to the present invention, these components can be included in lower levels than in currently available detergent compositions. Further non-limiting examples include citrate, chelators such as aminocarboxylates, aminopolycarboxylates and phosphonates, and alkyl- or alkenylsuccinic acid. Additional specific examples include 2,2’,2”-nitrilotriacetic acid (NTA), ethylenediaminetetraacetic acid (EDTA), diethylenetriaminepentaacetic acid (DTPA), iminodisuccinic acid (IDS), ethylenediamine-N,N’-disuccinic acid (EDDS), methylglycinediacetic acid (MGDA), glutamic acid-N,N -di acetic acid (GLDA), 1-hydroxyethane-1 ,1-diylbis(phosphonic acid (HEDP), ethylenediaminetetramethylenetetrakis(phosphonic acid)
(EDTMPA),diethylenetriaminepentamethylenepentakis(phosphonic acid) (DTMPA or DTPMPA), N- (2-hydroxyethyl)iminodiacetic acid (EDG), aspartic acid-N-monoacetic acid (ASMA), aspartic acid- N,N-diacetic acid (ASDA), aspartic acid-N-monopropionic acid (ASMP), iminodisuccinic acid (IDA), N-(2-sulfomethyl)aspartic acid (SMAS), N-(2-sulfoethyl)aspartic acid (SEAS), N-(2- sulfomethyl)glutamicacid (SMGL), N-(2-sulfoethyl)glutamicacid (SEGL), N-methyliminodiaceticacid (MIDA), a-alanine-N,N-diacetic acid (a-ALDA), serine-N,N-diacetic acid (SEDA), isoserine-N,N- diacetic acid (ISDA), phenylalanine-N,N-diacetic acid (PH DA), anthranilic acid-N,N-diacetic acid (ANDA), sulfanilicacid-N,N-diaceticacid (SLDA) , taurine-N,N-diaceticacid (TLIDA) and sulfomethyl- N,N-diacetic acid (SMDA), N-(2-hydroxyethyl)ethylenediamine-N,N’,N”-triacetic acid (HEDTA), diethanolglycine (DEG), aminotrimethylenetris(phosphonic acid) (ATMP), and combinations and salts thereof. Further exemplary builders and/or co-builders are described in, e.g., WO 09/102854 and US 5977053.
Bleaching Systems
The cleaning composition may contain 0-50% by weight, such as 1-40%, such as 1-30%, such as about 1% to about 20%, of a bleaching system. Any oxygen-based bleaching system comprising components known in the art for use in cleaning detergents may be utilized. Suitable bleaching system components include sources of hydrogen peroxide; peracids and sources of peracids (bleach activators); and bleach catalysts or boosters.
Sources of hydrogen peroxide: Suitable sources of hydrogen peroxide are inorganic persalts, including alkali metal salts such as sodium percarbonate and sodium perborates (usually mono- or tetrahydrate), and hydrogen peroxide— urea (1/1).
Sources of peracids: Peracids may be (a) incorporated directly as preformed peracids or (b) formed in situ in the wash liquor from hydrogen peroxide and a bleach activator (perhydrolysis) or (c) formed in situ in the wash liquor from hydrogen peroxide and a perhydrolase and a suitable substrate for the latter, e.g., an ester: a) Suitable preformed peracids include, but are not limited to, peroxycarboxylic acids such as peroxybenzoic acid and its ring-substituted derivatives, peroxy-a-naphthoic acid, peroxyphthalic acid, peroxylauric acid, peroxystearic acid, e-phthalimidoperoxycaproic acid [phthalimidoperoxyhexanoic acid (PAP)], and o-carboxybenzamidoperoxycaproic acid; aliphatic and aromatic diperoxydicarboxylic acids such as diperoxydodecanedioic acid, diperoxyazelaic acid, diperoxysebacic acid, diperoxybrassylic acid, 2- decyldiperoxybutanedioic acid, and diperoxyphthalic, -isophthalic and -terephthalic acids; perimidic acids; peroxymonosulfuric acid; peroxydisulfuric acid; peroxyphosphoric acid; peroxysilicic acid; and mixtures of said compounds. It is understood that the peracids mentioned may in some cases be best added as suitable salts, such as alkali metal salts (e.g., Oxone®) or alkaline earth-metal salts. b) Suitable bleach activators include those belonging to the class of esters, amides, imides, nitriles or anhydrides and, where applicable, salts thereof. Suitable examples are tetraacetylethylenediamine (TAED), sodium 4-[(3,5,5-trimethylhexanoyl)oxy]benzene-1- sulfonate (ISONOBS), sodium 4-(dodecanoyloxy)benzene-1-sulfonate (LOBS), sodium 4- (decanoyloxy)benzene-l -sulfonate, 4-(decanoyloxy)benzoic acid (DOBA), sodium 4- (nonanoyloxy) benzene- 1 -sulfonate (NOBS), and/or those disclosed in WO98/17767. A particular family of bleach activators of interest was disclosed in EP624154 and particularly preferred in that family is acetyl triethyl citrate (ATC). ATC or a short chain triglyceride like triacetin has the advantage that they are environmentally friendly. Furthermore, acetyl triethyl citrate and triacetin have good hydrolytical stability in the product upon storage and are efficient bleach activators. Finally, ATC is multifunctional, as the citrate released in the perhydrolysis reaction may function as a builder.
Bleach catalysts and boosters
The bleaching system may also include a bleach catalyst or booster.
Some non-limiting examples of bleach catalysts that may be used in the compositions of the present invention include manganese oxalate, manganese acetate, manganese-collagen, cobalt-amine catalysts and manganese triazacyclononane (MnTACN) catalysts; particularly preferred are complexes of manganese with 1 ,4,7-trimethyl-1 ,4,7-triazacyclononane (Me3- TACN) or 1 ,2,4,7-tetramethyl-1 ,4,7-triazacyclononane (Me4-TACN), in particular Me3-TACN, such as the dinuclear manganese complex [(Me3-TACN)Mn(O)3Mn(Me3-TACN)](PF6)2, and [2,2',2"-nitrilotris(ethane-1,2-diylazanylylidene-KN-methanylylidene)triphenolato- K30]manganese(lll). The bleach catalysts may also be other metal compounds, such as iron or cobalt complexes.
In some embodiments, where a source of a peracid is included, an organic bleach catalyst or bleach booster may be used having one of the following formulae:
(iii) and mixtures thereof; wherein each R1 is independently a branched alkyl group containing from 9 to 24 carbons or linear alkyl group containing from 11 to 24 carbons, preferably each R1 is independently a branched alkyl group containing from 9 to 18 carbons or linear alkyl group containing from 11 to 18 carbons, more preferably each R1 is independently selected from the group consisting of 2-propylheptyl, 2-butyloctyl, 2-pentylnonyl, 2-hexyldecyl, dodecyl, tetradecyl, hexadecyl, octadecyl, isononyl, isodecyl, isotridecyl and isopentadecyl.
Other exemplary bleaching systems are described, e.g. in W02007/087258, W02007/087244, W02007/087259, EP1867708 (Vitamin K) and W02007/087242. Suitable photobleaches may for example be sulfonated zinc or aluminium phthalocyanines.
Polymers and dispersants
Generally, detergent compositions may contain 0-10% by weight, such as 0.5-5%, 2-5%, 0.5-2% or 0.2-1 % of a polymer. Any polymer known in the art for use in detergents may be utilized. The polymer may function as a co-builder as mentioned above, or may provide anti-redeposition, fiber protection, soil release, dye transfer inhibition, grease cleaning and/or anti-foaming properties. Some polymers may have more than one of the above-mentioned properties and/or more than one of the below-mentioned motifs. Exemplary polymers include poly(vinyl alcohol) (PVA), poly(vinylpyrrolidone) (PVP), poly(ethyleneglycol) or poly(ethylene oxide) (PEG), ethoxylated poly(ethyleneimine), carboxymethyl inulin (CMI), and silicones, copolymers of terephthalic acid and oligomeric glycols, copolymers of poly(ethylene terephthalate) and poly(oxyethene terephthalate) (PET-POET), PVP, poly(vinylimidazole) (PVI), poly(vinylpyridine-/V- oxide) (PVPO or PVPNO) and polyvinylpyrrolidone-vinylimidazole (PVPVI). Further exemplary polymers include polyethylene oxide and polypropylene oxide (PEO-PPO), diquaternium ethoxy sulfate, styrene/acrylic copolymer and perfume capsules Other exemplary polymers are disclosed in, e.g., WO 2006/130575. Salts of the above-mentioned polymers are also contemplated.
The detergent compositions of the present invention can also contain dispersants. In particular powdered detergents may comprise dispersants. Suitable water-soluble organic materials include the homo- or co-polymeric acids or their salts, in which the polycarboxylic acid comprises at least two carboxyl radicals separated from each other by not more than two carbon atoms. Suitable dispersants are for example described in Powdered Detergents, Surfactant science series volume 71 , Marcel Dekker, Inc.
According to the present invention, however, certain of the above polymers, namely, a polyacrylic acid, a modified polyacrylic acid polymer, a modified polyacrylic acid copolymer, a maleic acid-acrylic acid copolymer, carboxymethyl cellulose, cellulose gum, methyl cellulose, and/or combinations thereof, can be included in lower levels than in currently available detergent compositions, or even more preferably, excluded altogether.
Fabric hueing agents
The detergent compositions of the present invention may also include fabric hueing agents such as dyes or pigments, which when formulated in detergent compositions can deposit onto a fabric when said fabric is contacted with a wash liguor comprising said detergent compositions and thus altering the tint of said fabric through absorption/reflection of visible light. Fluorescent whitening agents emit at least some visible light. In contrast, fabric hueing agents alter the tint of a surface as they absorb at least a portion of the visible light spectrum. Suitable fabric hueing agents include dyes and dye-clay conjugates and may also include pigments. Suitable dyes include small molecule dyes and polymeric dyes. Suitable small molecule dyes include small molecule dyes selected from the group consisting of dyes falling into the Colour Index (C.l.) classifications of Direct Blue, Direct Red, Direct Violet, Acid Blue, Acid Red, Acid Violet, Basic Blue, Basic Violet and Basic Red, or mixtures thereof, for example as described in W02005/03274, W02005/03275, W02005/03276 and EP1876226 (hereby incorporated by reference). The detergent composition preferably comprises from about 0.00003 wt% to about 0.2 wt%, from about 0.00008 wt% to about 0.05 wt%, or even from about 0.0001 wt% to about 0.04 wt% fabric hueing agent. The composition may comprise from 0.0001 wt% to 0.2 wt% fabric hueing agent, this may be especially preferred when the composition is in the form of a unit dose pouch. Suitable hueing agents are also disclosed in, e.g. WO 2007/087257 and W02007/087243.
Dye Transfer Inhibiting Agents
The detergent compositions of the present invention may also include one or more dye transfer inhibiting agents. Suitable polymeric dye transfer inhibiting agents include, but are not limited to, polyvinylpyrrolidone polymers, polyamine /V-oxide polymers, copolymers of /V- vinylpyrrolidone and /V-vinylimidazole, polyvinyloxazolidones and polyvinylimidazoles or mixtures thereof. When present in a subject composition, the dye transfer inhibiting agents may be present at levels from about 0.0001 % to about 10%, from about 0.01 % to about 5% or even from about 0.1 % to about 3% by weight of the composition. Fluorescent whitening agent
The detergent compositions of the present invention will preferably also contain additional components that may tint articles being cleaned, such as fluorescent whitening agent or optical brighteners. Where present the brightener is preferably at a level of about 0.01% to about 0.5%. Any fluorescent whitening agent suitable for use in a laundry detergent composition may be used in the composition of the present invention. The most commonly used fluorescent whitening agents are those belonging to the classes of diaminostilbene-sulfonic acid derivatives, diarylpyrazoline derivatives and bisphenyl-distyryl derivatives. Examples of the diaminostilbenesulfonic acid derivative type of fluorescent whitening agents include the sodium salts of: 4,4'-bis- (2-diethanolamino-4-anilino-s-triazin-6-ylamino) stilbene-2,2'-disulfonate, 4,4'-bis-(2,4-dianilino- s-triazin-6-ylamino) stilbene-2.2'-disulfonate, 4,4'-bis-(2-anilino-4-(/V-methyl-/\/-2-hydroxy- ethylamino)-s-triazin-6-ylamino) stilbene-2,2'-disulfonate, 4,4'-bis-(4-phenyl-1 ,2,3-triazol-2- yl)stilbene-2,2'-disulfonate and sodium 5-(2/7-naphtho[1 ,2-d][1 ,2,3]triazol-2-yl)-2-[(E)-2- phenylvinyl]benzenesulfonate. Preferred fluorescent whitening agents are Tinopal DMS and Tinopal CBS available from Ciba-Geigy AG, Basel, Switzerland. Tinopal DMS is the disodium salt of 4,4'-bis-(2-morpholino-4-anilino-s-triazin-6-ylamino) stilbene-2,2'-disulfonate. Tinopal CBS is the disodium salt of 2,2'-bis-(phenyl-styryl)-disulfonate. Also preferred are fluorescent whitening agents is the commercially available Parawhite KX, supplied by Paramount Minerals and Chemicals, Mumbai, India. Tinopal CBS-X is a 4.4'-bis-(sulfostyryl)-biphenyl disodium salt also known as Disodium Distyrylbiphenyl Disulfonate. Other fluorescers suitable for use in the invention include the 1 -3-diaryl pyrazolines and the 7-alkylaminocoumarins.
Suitable fluorescent brightener levels include lower levels of from about 0.01 , from 0.05, from about 0.1 or even from about 0.2 wt % to upper levels of 0.5 or even 0.75 wt%.
Soil release polymers
The detergent compositions of the present invention may also include one or more soil release polymers which aid the removal of soils from fabrics such as cotton and polyester based fabrics, in particular the removal of hydrophobic soils from polyester based fabrics. The soil release polymers may for example be nonionic or anionic terephthalte based polymers, polyvinyl caprolactam and related copolymers, vinyl graft copolymers, polyester polyamides see for example Chapter 7 in Powdered Detergents, Surfactant science series volume 71 , Marcel Dekker, Inc. Another type of soil release polymers are amphiphilic alkoxylated grease cleaning polymers comprising a core structure and a plurality of alkoxylate groups attached to that core structure. The core structure may comprise a polyalkylenimine structure or a polyalkanolamine structure as described in detail in WO 2009/087523 (hereby incorporated by reference). Furthermore, random graft co-polymers are suitable soil release polymers. Suitable graft co-polymers are described in more detail in WO 2007/138054, WO 2006/108856 and WO 2006/113314 (hereby incorporated by reference).
Anti-redeposition agents
The detergent compositions of the present invention may also include one or more antiredeposition agents such as carboxymethylcellulose (CMC), polyvinyl alcohol (PVA), polyoxyethylene and/or polyethyleneglycol (PEG), homopolymers of acrylic acid, copolymers of acrylic acid and maleic acid. The cellulose based polymers described under soil release polymers above may also function as anti-redeposition agents.
According to the present invention, however, certain of the above polymers, namely, a polyacrylic acid, a modified polyacrylic acid polymer, a modified polyacrylic acid copolymer, a maleic acid-acrylic acid copolymer, carboxymethyl cellulose, cellulose gum, methyl cellulose, and/or combinations thereof, can by introduction of cellulases in combination with mannanases be included in lower levels than in currently available detergent compositions, or excluded altogether, thus improving the sustainability profile of the detergent composition.
Rheology Modifiers
The detergent compositions of the present invention may also include one or more rheology modifiers, structurants or thickeners, as distinct from viscosity reducing agents. The rheology modifiers are selected from the group consisting of non-polymeric crystalline, hydroxyfunctional materials, polymeric rheology modifiers which impart shear thinning characteristics to the agueous liguid matrix of a liguid detergent composition. The rheology and viscosity of the detergent can be modified and adjusted by methods known in the art, for example as shown in EP 2169040.
Other suitable adjunct materials include, but are not limited to, anti-shrink agents, anti-wrinkling agents, bactericides, binders, carriers, dyes, enzyme stabilizers, fabric softeners, fillers, foam regulators, hydrotropes, perfumes, pigments, sod suppressors, solvents, and structurants for liguid detergents and/or structure elasticizing agents.
Additional Enzymes
The detergent additive as well as the detergent composition may comprise one or more enzymes in addition to the enzyme compositions of the present invention, such as - but not limited to - the enzymes described below. Typically, if the enzyme composition of the present invention is used in a laundry detergent protease and amylase will be added if not present in the enzyme composition. In general, the properties of the selected enzyme(s) should be compatible with the selected detergent, (/.e., pH-optimum, compatibility with other enzymatic and non-enzymatic ingredients, etc.), and the enzyme(s) should be present in effective amounts.
Cellulases
The term “cellulase” means one or more (e.g., several) enzymes that hydrolyze a cellulosic material and include endoglucanase(s) (e.g. EC 3.2.1.4), cellobiohydrolase(s), beta- glucosidase(s), or combinations thereof. Suitable cellulases include mono-component and mixtures of enzymes of bacterial or fungal origin. Chemically modified or protein engineered mutants are also contemplated. The cellulase may for example be a mono-component or a mixture of mono-component endo-1 ,4-beta-glucanase also referred to as endoglucanase.
WO 2023/061928 discloses cellulases with improved stability in the presence of proteases.
DNases (deoxyribonuclease)
The term “DNase” means a polypeptide with DNase activity that catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA backbone, thus degrading DNA.
Mannanases
Mannanase belongs to the glycoside hydrolase (GH) family 5 or family 26. These enzymes are involved in the hydrolysis of mannans, which are polysaccharides found in plant cell walls and are composed predominantly of mannose residues. Suitable mannanases are described in WO 1999/064619, WO 2018/206300 and WO 2023/247348.
Xanthanases
Xanthan lyase (EC 4.2.2.12) is an enzyme that catalyzes the chemical reaction of cleaving the beta-D-mannosyl-beta-D-1 ,4-glucuronosyl bond on the polysaccharide xanthan. This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on polysaccharides.
Xyloglucanase
Xyloglucanase enzymes (GH44) are involved in the hydrolysis of xyloglucan, a hemicellulose polysaccharide found in plant cell walls. Xyloglucanase enzymes play a crucial role in breaking down the complex structure of xyloglucan, making them important in various industrial and biological applications. Proteases
Suitable proteases may be of any origin, but are preferably of bacterial or fungal origin, optionally in the form of protein engineered or chemically modified mutants. The protease may be an alkaline protease, such as a serine protease or a metalloprotease. A serine protease may for example be of the S1 family, such as trypsin, or the S8 family such as a subtilisin. A metalloprotease may for example be a thermolysin, e.g. from the M4 family, or another metalloprotease such as those from the M5, M7 or M8 families.
The term "subtilases" refers to a sub-group of serine proteases according to Siezen et al., Protein Eng. 4 (1991) 719-737 and Siezen et al., Protein Sci. 6 (1997) 501-523. Serine proteases are a subgroup of proteases characterized by having a serine in the active site, which forms a covalent adduct with the substrate. The subtilases may be divided into six subdivisions, the Subtilisin family, the Thermitase family, the Proteinase K family, the Lantibiotic peptidase family, the Kexin family and the Pyrolysin family.
Although proteases suitable for detergent use may be obtained from a variety of organisms, including fungi such as Aspergillus, detergent proteases have generally been obtained from bacteria and in particular tromBacillus. Examples of Bacillus species from which subtilases have been derived include Bacillus lentus, Bacillus alkalophilus, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus pumilus and Bacillus gibsonii. Particular subtilisins include subtilisin lentus, subtilisin Novo, subtilisin Carlsberg, subtilisin BPN’, subtilisin 309, subtilisin 147 and subtilisin 168 and e.g. protease PD138 (described in WO 93/18140). Other useful proteases are e.g. those described in WO 01/16285 and WO 02/16547.
Examples of trypsin-like proteases include the Fusarium protease described in WO 94/25583 and WO 2005/040372, and the chymotrypsin proteases derived from Cellumonas described in WO 2005/052161 and WO 2005/052146.
Examples of metalloproteases include the neutral metalloproteases described in WO 2007/044993 such as those derived from Bacillus amyloliquefaciens, as well as e.g. the metalloproteases described in WO 2015/158723 and WO 2016/075078.
Examples of useful proteases are the protease variants described in WO 89/06279 WO 92/19729, WO 96/34946, WO 98/20115, WO 98/20116, WO 99/11768, WO 01/44452, WO 03/006602, WO 2004/003186, WO 2004/041979, WO 2007/006305, WO 2011/036263, WO 2014/207227, WO 2016/087617 and WO 2016/174234.
Suitable commercially available protease enzymes include those sold under the trade names Alcalase®, Duralase™, Durazym™, Relase®, Relase® Ultra, Savinase®, Savinase® Ultra, Primase™, Polarzyme®, Kannase®, Liquanase®, Liquanase® Ultra, Ovozyme®, Coronase®, Coronase® Ultra, Blaze®, Blaze Evity® 100T, Blaze Evity® 125T, Blaze Evity® 150T, Blaze Evity® 200T, Neutrase®, Everlase®, Esperase®, Progress® Uno, Progress® In and Progress® Excel (Novozymes A/S), those sold under the tradename Maxatase™, Maxacai™, Maxapem®, Purafect® Ox, Purafect® OxP, Puramax®, FN2™, FN3™, FN4ex™, Excellase®, Excellenz™ P1000, Excellenz™ P1250, Eraser™, Preferenz® P100, Purafect Prime, Preferenz P110™, Effectenz P1000™, Purafect®, Effectenz P1050™, Purafect® Ox, Effectenz ™ P2000, Purafast™, Properase®, Opticlean™ and Optimase® (Danisco/DuPont), BLAP (sequence shown in Figure 29 of US 5352604) and variants hereof (Henkel AG), and KAP (Bacillus alkalophilus subtilisin) from Kao.
Lipases and Cutinases
Suitable lipases and cutinases include those of bacterial or fungal origin. Chemically modified or protein engineered mutant enzymes are included. Examples include lipase from Thermomyces, e.g. from T. lanuginosus (previously named Humicola lanuginosa) as described in EP258068 and EP305216, cutinase from Humicola, e.g. H. insolens (WO96/13580), lipase from strains of Pseudomonas (some of these now renamed to Burkholderia), e.g. P. alcaligenes or P. pseudoalcaligenes (EP218272), P. cepacia (EP331376), P. sp. strain SD705 (W095/06720 & W096/27002), P. wisconsinensis (WO96/12012), GDSL-type Streptomyces lipases (W010/065455), cutinase from Magnaporthe grisea (WO10/107560), cutinase from Pseudomonas mendocina (US5,389,536), lipase from Thermobifida fusca (W011/084412), Geobacillus stearothermophilus lipase (W011/084417), lipase from Bacillus subtilis (W011/084599), and lipase from Streptomyces griseus (WO11/150157) and S. pristinaespiralis (W012/137147).
Other examples are lipase variants such as those described in EP407225, WO92/05249, WO94/01541 , WO94/25578, WO95/14783, WO95/30744, WO95/35381 , WO95/22615,
W096/00292, W097/04079, W097/07202, WO00/34450, WO00/60063, W001/92502,
W007/87508 and WO09/109500.
Preferred commercial lipase products include include Lipolase 100T/L, Lipex 100T/L, Lipex 105T, Lipex Evity 100L, Lipex Evity 200L (all Novozymes A/S), Preferenz® L 100 (DuPont).
Still other examples are lipases sometimes referred to as acyltransferases or perhydrolases, e.g. acyltransferases with homology to Candida antarctica lipase A (WO10/111143), acyltransferase from Mycobacterium smegmatis (WO05/56782), perhydrolases from the CE 7 family (WO09/67279), and variants of the M. smegmatis perhydrolase in particular the S54V variant used in the commercial product Gentle Power Bleach from Huntsman Textile Effects Pte Ltd (W010/100028). Amylases
Suitable amylases include an alpha-amylase or a glucoamylase and may be of bacterial or fungal origin. Chemically modified or protein engineered mutants are included. Amylases include, for example, alpha-amylases obtained from Bacillus, e.g., a special strain of Bacillus licheniformis, described in more detail in GB 1 ,296,839.
Suitable amylases include amylases having SEQ ID NO:2 in WO 95/10603 or variants having 90% sequence identity to SEQ ID NO:3 thereof. Preferred variants are described in WO 94/02597, WO 94/18314, WO 97/43424 and SEQ ID NO:4 of WO 99/019467, such as variants with substitutions in one or more of the following positions: 15, 23, 105, 106, 124, 128, 133, 154, 156, 178, 179, 181 , 188, 190, 197, 201 , 202, 207, 208, 209, 211 , 243, 264, 304, 305, 391 , 408, and 444.
Different suitable amylases include amylases having SEQ ID NO:6 in WO 02/010355 or variants thereof having 90% sequence identity to SEQ ID NO:6. Preferred variants of SEQ ID NO:6 are those having a deletion in positions 181 and 182 and a substitution in position 193.
Other amylases which are suitable are hybrid alpha-amylase comprising residues 1-33 of the alpha-amylase derived from B. amyloliquefaciens shown in SEQ ID NO:6 of WO 2006/066594 and residues 36-483 of the B. licheniformis alpha-amylase shown in SEQ ID NO:4 of WO 2006/066594 or variants having 90% sequence identity thereof.
Other examples are amylase variants such as those described in WO2011/098531 , WO2013/001078 and WO2013/001087.
Commercially available amylases are Duramyl™, Termamyl™, Fungamyl™, Stainzyme TM, Stainzyme Plus™, Natalase™, Liquozyme X and BAN™ Amplify; Amplify Prime; (from Novozymes A/S), and Rapidase™ , Purastar™/Effectenz™, Powerase, Preferenz S1000, Preferenz S100 and Preferenz S110 (from Genencor International Inc./DuPont).
Peroxidases/Oxidases
Suitable peroxidases/oxidases include those of plant, bacterial or fungal origin. Chemically modified or protein engineered mutants are included. Examples of useful peroxidases include peroxidases from Coprinus, e.g., from C. cinereus, and variants thereof as those described in WO 93/24618, WO 95/10602, and WO 98/15257. Commercially available peroxidases include Guardzyme™ (Novozymes A/S).
A suitable peroxidase is preferably a peroxidase enzyme comprised by the enzyme classification EC 1.11.1.7, as set out by the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology (IUBMB), or any fragment derived therefrom, exhibiting peroxidase activity. Suitable peroxidases also include a haloperoxidase enzyme, such as chloroperoxidase, bromoperoxidase and compounds exhibiting chloroperoxidase or bromoperoxidase activity. Haloperoxidases are classified according to their specificity for halide ions. Chloroperoxidases (E.C. 1.11.1.10) catalyze formation of hypochlorite from chloride ions. The haloperoxidase may be a chloroperoxidase. Preferably, the haloperoxidase is a vanadium haloperoxidase, i.e., a vanadate-containing haloperoxidase. In a preferred method the vanadate-containing haloperoxidase is combined with a source of chloride ion.
Haloperoxidases have been isolated from many different fungi, in particular from the fungus group dematiaceous hyphomycetes, such as Caldariomyces, e.g., C. fumago, Alternaria, Curvularia, e.g., C. verruculosa and C. inaequalis, Drechslera, Ulocladium and Botrytis.
Haloperoxidases have also been isolated from bacteria such as Pseudomonas, e.g., P. pyrrocinia and Streptomyces, e.g., S. aureofaciens.
The haloperoxidase may be derivable from Curvularia sp., in particular Curvularia verruculosa or Curvularia inaequalis, such as C. inaequalis CBS 102.42 as described in WO 95/27046; or C. verruculosa CBS 147.63 or C. verruculosa CBS 444.70 as described in WO 97/04102; or from Drechslera hartlebii as described in WO 01/79459, Dendryphiella salina as described in WO 01/79458, Phaeotrichoconis crotalarie as described in WO 01/79461 , or Geniculosporium sp. as described in WO 01/79460.
Suitable oxidases include, in particular, any laccase enzyme comprised by the enzyme classification EC 1.10.3.2, or any fragment derived therefrom exhibiting laccase activity, or a compound exhibiting a similar activity, such as a catechol oxidase (EC 1.10.3.1), an o- aminophenol oxidase (EC 1.10.3.4), or a bilirubin oxidase (EC 1.3.3.5).
Preferred laccase enzymes are enzymes of microbial origin. The enzymes may be derived from plants, bacteria or fungi (including filamentous fungi and yeasts).
Suitable examples from fungi include a laccase derivable from a strain of Aspergillus, Neurospora, e.g., N. crassa, Podospora, Botrytis, Collybia, Pomes, Lentinus, Pleurotus, Trametes, e.g., T. villosa and T. versicolor, Rhizoctonia, e.g., R. solani, Coprinopsis, e.g., C. cinerea, C. comatus, C. friesii, and C. plicatilis, Psathyrella, e.g., P. condelleana, Panaeolus, e.g., P. papilionaceus, Myceliophthora, e.g., M. thermophila, Schytalidium, e.g., S. thermophilum, Polyporus, e.g., P. pinsitus, Phlebia, e.g., P. radiata (WO 92/01046), or Coriolus, e.g., C. hirsutus (JP 2238885).
Suitable examples from bacteria include a laccase derivable from a strain of Bacillus.
A laccase derived from Coprinopsis or Myceliophthora is preferred; in particular a laccase derived from Coprinopsis cinerea, as disclosed in WO 97/08325; or from Myceliophthora thermophila, as disclosed in WO 95/33836. Laundry soap bars
The proteases of the invention may be added to laundry soap bars and used for hand washing laundry, fabrics and/or textiles. The term laundry soap bar includes laundry bars, soap bars, combo bars, syndet bars and detergent bars. The types of bar usually differ in the type of surfactant they contain, and the term laundry soap bar includes those containing soaps from fatty acids and/or synthetic soaps. The laundry soap bar has a physical form which is solid and not a liquid, gel or a powder at room temperature. The term solid is defined as a physical form which does not significantly change over time, i.e. if a solid object (e.g. laundry soap bar) is placed inside a container, the solid object does not change to fill the container it is placed in. The bar is a solid typically in bar form but can be in other solid shapes such as round or oval.
The laundry soap bar may contain one or more additional enzymes, protease inhibitors such as peptide aldehydes (or hydrosulfite adduct or hemiacetal adduct), boric acid, borate, borax and/or phenylboronic acid derivatives such as 4-formylphenylboronic acid, one or more soaps or synthetic surfactants, polyols such as glycerine, pH controlling compounds such as fatty acids, citric acid, acetic acid and/or formic acid, and/or a salt of a monovalent cation and an organic anion wherein the monovalent cation may be for example Na+, K+ or NH4+ and the organic anion may be for example formate, acetate, citrate or lactate such that the salt of a monovalent cation and an organic anion may be, for example, sodium formate.
The laundry soap bar may also contain complexing agents like EDTA and HEDP, perfumes and/or different type of fillers, surfactants e.g. anionic synthetic surfactants, builders, polymeric soil release agents, detergent chelators, stabilizing agents, fillers, dyes, colorants, dye transfer inhibitors, alkoxylated polycarbonates, suds suppressers, structurants, binders, leaching agents, bleaching activators, clay soil removal agents, anti-redeposition agents, polymeric dispersing agents, brighteners, fabric softeners, perfumes and/or other compounds known in the art.
The laundry soap bar may be processed in conventional laundry soap bar making equipment such as, but not limited to, mixers, plodders, e.g., a two-stage vacuum plodder, extruders, cutters, logo-stampers, cooling tunnels and wrappers. The invention is not limited to preparing the laundry soap bars by any single method. The premix of the invention may be added to the soap at different stages of the process. For example, the premix containing a soap, proteases, optionally one or more additional enzymes, a protease inhibitor, and a salt of a monovalent cation and an organic anion may be prepared, and the mixture is then plodded. The proteases and optional additional enzymes may be added at the same time as the protease inhibitor for example in liquid form. Besides the mixing step and the plodding step, the process may further comprise the steps of milling, extruding, cutting, stamping, cooling and/or wrapping. Formulation of detergent products
The detergent composition of the invention may be in any convenient form, e.g., a bar, a homogenous tablet, a tablet having two or more layers, a pouch having one or more compartments, a regular or compact powder, a granule, a paste, a gel, or a regular, compact or concentrated liquid. Pouches can be configured as single or multi compartments. It can be of any form, shape and material which is suitable for hold the composition, e.g., without allowing the release of the composition to release of the composition from the pouch prior to water contact. The pouch is made from water soluble film which encloses an inner volume. Said inner volume can be divided into compartments of the pouch. Preferred films are polymeric materials preferably polymers which are formed into a film or sheet. Preferred polymers, copolymers or derivates thereof are selected polyacrylates, and water-soluble acrylate copolymers, methyl cellulose, carboxy methyl cellulose, sodium dextrin, ethyl cellulose, hydroxyethyl cellulose, hydroxypropyl methyl cellulose, maltodextrin, poly methacrylates, most preferably polyvinyl alcohol copolymers and, hydroxypropyl methyl cellulose (HPMC). Preferably the level of polymer in the film for example PVA is at least about 60%. Preferred average molecular weight will typically be about 20,000 to about 150,000. Films can also be of blended compositions comprising hydrolytically degradable and water-soluble polymer blends such as polylactide and polyvinyl alcohol (known under the Trade reference M8630 as sold by MonoSol LLC, Indiana, USA) plus plasticisers like glycerol, ethylene glycerol, propylene glycol, sorbitol and mixtures thereof. The pouches can comprise a solid laundry cleaning composition or part components and/or a liquid cleaning composition or part components separated by the water- soluble film. The compartment for liquid components can be different in composition than compartments containing solids: US2009/0011970 A1.
Detergent ingredients can be separated physically from each other by compartments in water dissolvable pouches or in different layers of tablets. Thereby negative storage interaction between components can be avoided. Different dissolution profiles of each of the compartments can also give rise to delayed dissolution of selected components in the wash solution.
A liquid or gel detergent, which is not unit dosed, may be aqueous, typically containing at least 20% by weight and up to 95% water, such as up to about 70% water, up to about 65% water, up to about 55% water, up to about 45% water, up to about 35% water. Other types of liquids, including without limitation, alkanols, amines, diols, ethers and polyols may be included in an aqueous liquid or gel. An aqueous liquid or gel detergent may contain from 0-30% organic solvent. A liquid or gel detergent may be non-aqueous.
Sequences
Embodiments of the invention
The invention is further summarized in the following embodiments. The embodiments are indicated as 1 , 2 and so forth.
1 . A composition comprising two or more enzymes selected from the group consisting of a. A protease having at least 80% sequence identity to SEQ ID NO:1 ; b. A protease having at least 80% sequence identity to SEQ ID NO:2; c. A protease having at least 80% sequence identity to SEQ ID NO:3; d. A lipase having at least 80% sequence identity to SEQ ID NO:4; e. An amylase having at least 80% sequence identity to SEQ ID NO:5; f. A mannanase having at least 80% sequence identity to SEQ ID NO:6 g. A mannanase having at least 80% sequence identity to SEQ ID NO:7; h. A xyloglucanase having at least 80% sequence identity to SEQ ID NO:8; i. A cellulase having at least 80% sequence identity to SEQ ID NO:9; j. A pectinase having at least 80% sequence identity to SEQ ID NO: 10; k. A DNase having at least 80% sequence identity to SEQ ID NO:11 ; l. A cellulase having at least 80% sequence identity to SEQ ID NO:12; m. A protease having at least 80% sequence identity to SEQ ID NO: 13; and n. A protease having at least 80% sequence identity to SEQ ID NO:14.
2. A composition according to embodiment 1 , wherein the composition is selected from the group consisting of a. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14 and an amylase having at least 80% sequence identity to SEQ ID NO:5; b. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14 and a lipase having at least 80% sequence identity to SEQ ID NO:4; c. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14 and DNase having at least 80% sequence identity to SEQ ID NO:11 ; d. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14 and a cellulase having at least 80% sequence identity to SEQ ID NO:9; e. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14 and a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8; f. A protease having at least 80% sequence identity to any of SEQ ID NO:3, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:1 , or SEQ ID NO:2, a pectinase having at least 80% sequence identity to SEQ ID NO: 10, and an amylase having at least 80% sequence identity to SEQ ID NO:5: g. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ I D NO:6 or SEQ I D NO: 7, and an amylase having at least 80% sequence identity to SEQ ID NO:5: h. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, an amylase having at least 80% sequence identity to SEQ ID NO:5, and a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8; i. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6 or SEQ ID NO: 7, a pectinase having at least 80% sequence identity to SEQ ID NO: 10, a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8 and an amylase having at least 80% sequence identity to SEQ ID NO:5; j. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6 or SEQ ID NO: 7, a pectinase having at least 80% sequence identity to SEQ ID NO: 10, a lipase having at least 80% sequence identity to SEQ ID NO:4 and an amylase having at least 80% sequence identity to SEQ ID NO:5; k. A protease having at least 80% sequence identity to any of SEQ I D NO: 1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6 or SEQ ID NO: 7, a lipase having at least 80% sequence identity to SEQ ID NO:4, a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8 and an amylase having at least 80% sequence identity to SEQ ID NO:5; l. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6 or SEQ ID NO: 7, a lipase having at least 80% sequence identity to SEQ ID NO:4, a pectinase having at least 80% sequence identity to SEQ ID NO: 10, a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8, a cellulase having at least 80% sequence identity to SEQ ID NO:9, and an amylase having at least 80% sequence identity to SEQ ID NO:5; m. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6 or SEQ ID NO: 7, a lipase having at least 80% sequence identity to SEQ ID NO:4, a pectinase having at least 80% sequence identity to SEQ ID NO: 10, a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8, a cellulase having at least 80% sequence identity to SEQ ID NO:9, a DNase having at least 80% sequence identity to SEQ ID NO:11 , and an amylase having at least 80% sequence identity to SEQ ID NO:5; n. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6 or SEQ ID NO: 7, a lipase having at least 80% sequence identity to SEQ ID NO:4, a pectinase having at least 80% sequence identity to SEQ ID NO: 10, a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8, a DNase having at least 80% sequence identity to SEQ ID NO:11 , and an amylase having at least 80% sequence identity to SEQ ID NO:5; o. A protease having at least 80% sequence identity to any of SEQ I D NO: 1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6 or SEQ ID NO: 7, a pectinase having at least 80% sequence identity to SEQ ID NO: 10, a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8, a cellulase having at least 80% sequence identity to SEQ ID NO:9, a DNase having at least 80% sequence identity to SEQ ID NO:11 , and an amylase having at least 80% sequence identity to SEQ ID NO:5; p. A cellulase having at least 80% sequence identity to SEQ ID NO:9 or SEQ ID NO: 12 and a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8. q. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6 or SEQ ID NO: 7, a lipase having at least 80% sequence identity to SEQ ID NO:4, and an amylase having at least 80% sequence identity to SEQ ID NO:5; r. A protease having at least 80% sequence identity to any of SEQ I D NO: 1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a lipase having at least 80% sequence identity to SEQ ID NO:4, and an amylase having at least 80% sequence identity to SEQ ID NO:5; and s. A protease having at least 80% sequence identity to any of SEQ I D NO: 1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a lipase having at least 80% sequence identity to SEQ ID NO:4, a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8, and an amylase having at least 80% sequence identity to SEQ ID NO:5. A composition according to embodiment 2, wherein the composition is selected from the group consisting of a. A cellulase having at least 80% sequence identity to SEQ ID NO:9 and a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8; b. A protease having at least 80% sequence identity to any of SEQ I D NO: 1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6, a mannanase having at least 80% sequence identity to SEQ ID NO: 7, and an amylase having at least 80% sequence identity to SEQ ID NO:5: c. A protease having at least 80% sequence identity to any of SEQ I D NO: 1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6, a mannanase having at least 80% sequence identity to SEQ ID NO: 7, a pectinase having at least 80% sequence identity to SEQ ID NO: 10, a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8 and an amylase having at least 80% sequence identity to SEQ ID NO:5; d. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6, a mannanase having at least 80% sequence identity to SEQ ID NO: 7, a pectinase having at least 80% sequence identity to SEQ ID NO: 10, a lipase having at least 80% sequence identity to SEQ ID NO:4 and an amylase having at least 80% sequence identity to SEQ ID NO:5; e. A protease having at least 80% sequence identity to any of SEQ I D NO: 1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6, a mannanase having at least 80% sequence identity to SEQ ID NO: 7, a lipase having at least 80% sequence identity to SEQ ID NO:4, a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8 and an amylase having at least 80% sequence identity to SEQ ID NO:5; f. A protease having at least 80% sequence identity to any of SEQ I D NO: 1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6, a mannanase having at least 80% sequence identity to SEQ ID NO: 7, a lipase having at least 80% sequence identity to SEQ ID NO:4, a pectinase having at least 80% sequence identity to SEQ ID NO: 10, a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8, a cellulase having at least 80% sequence identity to SEQ ID NO:9, and an amylase having at least 80% sequence identity to SEQ ID NO:5; g. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6, a mannanase having at least 80% sequence identity to SEQ ID NO: 7, a lipase having at least 80% sequence identity to SEQ ID NO:4, a pectinase having at least 80% sequence identity to SEQ ID NO: 10, a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8, a cellulase having at least 80% sequence identity to SEQ ID NO:9, a DNase having at least 80% sequence identity to SEQ ID NO:11 , and an amylase having at least 80% sequence identity to SEQ ID NO:5; h. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6, a mannanase having at least 80% sequence identity to SEQ ID NO: 7, a lipase having at least 80% sequence identity to SEQ ID NO:4, a pectinase having at least 80% sequence identity to SEQ ID NO: 10, a xyloglucanase having at least 80% sequence identity to SEQ ID N0:8, a DNase having at least 80% sequence identity to SEQ ID NO:11 , and an amylase having at least 80% sequence identity to SEQ ID NO:5; i. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6, a mannanase having at least 80% sequence identity to SEQ ID NO: 7, a pectinase having at least 80% sequence identity to SEQ ID NO: 10, a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8, a cellulase having at least 80% sequence identity to SEQ ID NO:9, a DNase having at least 80% sequence identity to SEQ ID NO:11 , and an amylase having at least 80% sequence identity to SEQ ID NO:5; and j. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6, a mannanase having at least 80% sequence identity to SEQ ID NO: 7, a lipase having at least 80% sequence identity to SEQ ID NO:4, and an amylase having at least 80% sequence identity to SEQ ID NO:5.
4. A detergent composition comprising the enzyme composition according to any of embodiments 1 to 3 and at least one surfactant.
5. The detergent composition according to embodiment 4 comprising 0.05-20 wt% rhamnolipid, such as 1-15 wt% rhamnolipid, such as 2-10 wt% rhamnolipid, such as 3-9 wt% rhamnolipid, such as 4-8 wt% rhamnolipid.
6. The detergent composition according to embodiment 5, wherein at least 90 wt% of the rhamnolipid is mono rhamnolipid.
7. The detergent composition according to any of embodiments 4 to 6, further comprising one or more additional enzymes selected from the group consisting of proteases, amylases, deoxyribonucleases, xyloglucanases, pectinases, pectin lyases, xanthan lyases, xanthan endoglucanases, peroxidases, haloperoxygenases, cellulases, licheninase, lipases, cutinases, catalases, oxidase, arabinose, galactanase and mannanases.
8. A method for removal of a stain on a textile during a wash cycle comprising contacting the textile with the detergent composition of any of embodiments 4 to 7.
9. A washing method for textile comprising: a. Exposing a textile to a wash liquor, said wash liquor comprising detergent composition according to any of embodiments 4 to 7; b. completing at least one wash cycle, and c. optionally rinsing the textile. 10. The washing method according to embodiment 9, wherein the temperature of the wash liquor is in the range of 5°C to 90°C, or in the range of 10°C to 80°C, or in the range of 10°C to 70°C, or in the range of 10°C to 60°C, or in the range of 10°C to 50°C, or in the range of 15°C to 40°C, or in the range of 20°C to 30°C.
11. Use of the detergent composition according to any of embodiments 1 to 7 for removal of a stain on textile in a wash liquor, wherein the temperature of the wash liquor is in the range of 5°C to 90°C, or in the range of 10°C to 80°C, or in the range of 10°C to 70°C, or in the range of 10°C to 60°C, or in the range of 10°C to 50°C, or in the range of 15°C to 40°C, or in the range of 20°C to 30°C.
Detergent compositions The below mentioned ranges of detergent components are generally useful.
Composition 1 : Liquid detergent
Composition 2: Unit Dose
Composition 3: Powder detergent
In one embodiment, the invention is directed to an ADW (Automatic Dish Wash) compositions comprising an enzyme of the present invention in combination with one or more additional ADW composition components. Surfactant ingredients can be obtained from BASF, Ludwigshafen, Germany (Lutensol®); Shell Chemicals, London, UK; Stepan, Northfield, III, USA; Huntsman, Huntsman, Salt Lake City, Utah, USA; Clariant, Sulzbach, Germany (Praepagen®).
Sodium tripolyphosphate can be obtained from Rhodia, Paris, France.
Zeolite can be obtained from Industrial Zeolite (UK) Ltd, Grays, Essex, UK.
Citric acid and sodium citrate can be obtained from Jungbunzlauer, Basel, Switzerland.
NOBS is sodium nonanoyloxybenzenesulfonate, supplied by Eastman, Batesville, Ark., USA.
TAED is tetraacetylethylenediamine, supplied under the Peractive® brand name by Clariant GmbH, Sulzbach, Germany.
Sodium carbonate and sodium bicarbonate can be obtained from Solvay, Brussels, Belgium.
Polyacrylate, polyacrylate/maleate copolymers can be obtained from BASF, Ludwigshafen, Germany.
Repel-O-Tex® can be obtained from Rhodia, Paris, France.
Texcare® can be obtained from Clariant, Sulzbach, Germany. Sodium percarbonate and sodium carbonate can be obtained from Solvay, Houston, Tex., USA.
Na salt of Ethylenediamine-N,N'-disuccinic acid, (S,S) isomer (EDDS) was supplied by Octel, Ellesmere Port, UK.
Hydroxy ethane di phosphonate (HEDP) was supplied by Dow Chemical, Midland, Mich., USA.
Enzymes Savinase®, Savinase® Ultra, Stainzyme® Plus, Lipex®, Lipolex®, Lipoclean®, Celluclean®, Carezyme®, Natalase®, Stainzyme®, Stainzyme® Plus, Termamyl®, Termamyl® ultra, and Mannaway® can be obtained from Novozymes, Bagsvaerd, Denmark.
Enzymes Purafect®, FN3, FN4 and Optisize can be obtained from Genencor International Inc., Palo Alto, California, US.
Direct violet 9 and 99 can be obtained from BASF DE, Ludwigshafen, Germany.
Solvent violet 13 can be obtained from Ningbo Lixing Chemical Co., Ltd. Ningbo, Zhejiang, China. Brighteners can be obtained from Ciba Specialty Chemicals, Basel, Switzerland.
All percentages and ratios are calculated by weight unless otherwise indicated. All percentages and ratios are calculated based on active concentration of the total composition unless otherwise indicated.
It should be understood that every maximum numerical limitation given throughout this specification includes every lower numerical limitation, as if such lower numerical limitations were expressly written herein. Every minimum numerical limitation given throughout this specification will include every higher numerical limitation, as if such higher numerical limitations were expressly written herein. Every numerical range given throughout this specification will include every narrower numerical range that falls within such broader numerical range, as if such narrower numerical ranges were all expressly written herein.
EXAMPLES
Example 1 : Stain removal and whiteness Impact of cellulases and mannanases are tested in a tergotometer, which is medium scale laboratory washing equipment for up to 16 stirred beakers of 1 L capacity. Below is described the ingredients and methods used in the test.
Preparation of Model detergent 1 :
Table 1 : Model detergent 1 Detergent Model 1 is produced by mixing water, propylene glycol, triethanolamine and sodium hydroxide. Then topped palm kernel fatty acid, Na-LAS, SLES and AEO is added in portions and stirred for few hours. Trisodium citrate dihydrate, DTPMP Na7 and 2-phenoxyethanol is added and pH is adjusted within target after overnight stirring at room temperature.
Stains in this example:
Table 2: Stained swatches In addition, 5x5 cm pre-washed CN42 (knitted cotton interlock, double jersey) is used for measuring redeposition of soil (whiteness tracer)
Enzymes in this example:
As background to all treatments are added the following commercial enzymes:
• Protease: 1.3 mg AEP/L wash liquor SEQ ID NO: 15, and • 0.1 mg AEP/L wash liquor SEQ ID NO: 5.
Both enzymes are available from Novonesis A/S.
Enzymes tested for synergistic effect:
Table 3: Enzymes tested in Example 1
Conditions for evaluation of wash performance in Terqotometer (TOM):
Table 4: Wash performance conditions, Example 1
The level of stain removal is measured by Remission at 460nm (REM460). A higher REM460 corresponds to a higher level of stain removal. Under similar wash parameters an enzyme reaches a maximum level of stain removal even at increasing dosage level of this enzyme. If combination with another enzyme makes it possible to raise the bar to even higher REM460 levels, then this is considered synergistic effects.
Results REM460 for three stains which responds very well to mannanase activity is showing synergistic effect with different cellulases. Enzyme dosage is given as mg active enzyme protein (AEP)/L wash liquor. The dosage level given for Mannanase A and B is at a level where maximum stain removal is observed under the given wash conditions. To all treatments are added background enzymes
Table 5: Results, Example 1
Surprisingly both Mannanase A and B show synergistic effect together with Cellulase A and B as well as with Xyloglucanase.
Example 2: Prevention of redeposition A redeposition test was used to evaluate synergism between cellulase, xyloglucanase and mannanase. Soil which is released into the wash liquor during the washing process may redeposit on fabric resulting in discoloration or greying. To simulate this phenomenon then white fabric was added to a 500 ml detergent liquor and chocolate ice cream was added as soil. The ability of cellulases and mannanases to prevent deposition of the ice cream on the white fabric were tested then using a tergotometer. A detailed of description of the ingredient and protocol is found below.
Materials and methods Model detergent 1 as in Example 1
White fabric applied in this example:
Table 6: Swatches
CN-42 fabric was prewashed and cut in 5x5 cm Enzymes in this example:
Mannanase, cellulase and xyloglucanase were included in the redeposition test. Dose levels were as stated in result table.
Table 7: Enzymes tested in Example 2 Conditions for evaluation of wash performance in Tergotometer (TOM):
Table 8: Wash performance conditions, Example 2
The level of redeposition was measured using a colorimeter where the Y component corresponds to the brightness or lightness of a color as perceived by the human eye (CIE 1931 XYZ color space). A higher Y value appear brighter and whiter and hence less redeposition. Results
Table 9: Results, Example 2
Cellulase A had no impact on redeposition when added alone, however surprisingly showed improved brightness in combination with Mannanase A. Xyloglucanase improved brightness when tested alone but showed even higher brightness in combination with Mannanase A.

Claims

1. A detergent composition comprising at least one surfactant, a mannanase or a blend of mannanases and a further enzyme selected from the group consisting of a. a xyloglucanase having at least 80% sequence identity to SEQ ID NO: 8, b. a cellulase having at least 80% sequence identity to SEQ ID NO: 9, and c. a cellulase having at least 80% sequence identity to SEQ ID NO: 12.
2. The detergent composition according to claim 1, wherein the mannanase is selected from the group consisting of a. a mannanase having at least 80% sequence identity to SEQ ID NO: 7 b. a mannanase having at least 80% sequence identity to SEQ ID NO: 6, and c. a mannanase having at least 80% sequence identity to SEQ ID NO: 7 and a mannanase having at least 80% sequence identity to SEQ ID NO: 6
3. The detergent composition according to claim 1 or 2, wherein said composition comprises a mannanase having at least 80% sequence identity to SEQ ID NO: 7 and a further enzyme selected from the group consisting of a. a xyloglucanase having at least 80% sequence identity to SEQ ID NO: 8, and b. a cellulase having at least 80% sequence identity to SEQ ID NO: 12.
4. Use of the detergent composition according to any of claims 1 to 3 for the improvement of prevention of redeposition during laundering.
5. A composition comprising two or more enzymes selected from the group consisting of a. A protease having at least 80% sequence identity to SEQ ID NO:1 ; b. A protease having at least 80% sequence identity to SEQ ID NO:2; c. A protease having at least 80% sequence identity to SEQ ID NO:3; d. A lipase having at least 80% sequence identity to SEQ ID NO:4; e. An amylase having at least 80% sequence identity to SEQ ID NO:5; f. A mannanase having at least 80% sequence identity to SEQ ID NO:6 g. A mannanase having at least 80% sequence identity to SEQ ID NO:7; h. A xyloglucanase having at least 80% sequence identity to SEQ ID NO:8; i. A cellulase having at least 80% sequence identity to SEQ ID NO:9; j. A pectinase having at least 80% sequence identity to SEQ ID NO: 10; k. A DNase having at least 80% sequence identity to SEQ ID NO:11 ; l. A cellulase having at least 80% sequence identity to SEQ ID NO: 12; m. A protease having at least 80% sequence identity to SEQ ID NO: 13; and n. A protease having at least 80% sequence identity to SEQ ID NO:14.
6. A composition according to claim 5, wherein the composition is selected from the group consisting of a. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14 and an amylase having at least 80% sequence identity to SEQ ID NO:5; b. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14 and a lipase having at least 80% sequence identity to SEQ ID NO:4; c. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14 and DNase having at least 80% sequence identity to SEQ ID NO:11 ; d. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14 and a cellulase having at least 80% sequence identity to SEQ ID NO:9; e. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14 and a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8; f. A protease having at least 80% sequence identity to any of SEQ ID NO:3, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:1 , or SEQ ID NO:2, a pectinase having at least 80% sequence identity to SEQ ID NO: 10, and an amylase having at least 80% sequence identity to SEQ ID NO:5: g. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ I D NO:6 or SEQ I D NO: 7, and an amylase having at least 80% sequence identity to SEQ ID NO:5: h. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, an amylase having at least 80% sequence identity to SEQ ID NO:5, and a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8; i. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6 or SEQ ID NO: 7, a pectinase having at least 80% sequence identity to SEQ ID NO: 10, a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8 and an amylase having at least 80% sequence identity to SEQ ID NO:5; j. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6 or SEQ ID NO: 7, a pectinase having at least 80% sequence identity to SEQ ID NO: 10, a lipase having at least 80% sequence identity to SEQ ID NO:4 and an amylase having at least 80% sequence identity to SEQ ID NO:5; k. A protease having at least 80% sequence identity to any of SEQ I D NO: 1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6 or SEQ ID NO: 7, a lipase having at least 80% sequence identity to SEQ ID NO:4, a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8 and an amylase having at least 80% sequence identity to SEQ ID NO:5; l. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6 or SEQ ID NO: 7, a lipase having at least 80% sequence identity to SEQ ID NO:4, a pectinase having at least 80% sequence identity to SEQ ID NO: 10, a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8, a cellulase having at least 80% sequence identity to SEQ ID NO:9, and an amylase having at least 80% sequence identity to SEQ ID NO:5; m. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6 or SEQ ID NO: 7, a lipase having at least 80% sequence identity to SEQ ID NO:4, a pectinase having at least 80% sequence identity to SEQ ID NO: 10, a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8, a cellulase having at least 80% sequence identity to SEQ ID NO:9, a DNase having at least 80% sequence identity to SEQ ID NO:11 , and an amylase having at least 80% sequence identity to SEQ ID NO:5; n. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6 or SEQ ID NO: 7, a lipase having at least 80% sequence identity to SEQ ID NO:4, a pectinase having at least 80% sequence identity to SEQ ID NO: 10, a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8, a DNase having at least 80% sequence identity to SEQ ID NO:11 , and an amylase having at least 80% sequence identity to SEQ ID NO:5; o. A protease having at least 80% sequence identity to any of SEQ I D NO: 1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6 or SEQ ID NO: 7, a pectinase having at least 80% sequence identity to SEQ ID NO: 10, a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8, a cellulase having at least 80% sequence identity to SEQ ID NO:9, a DNase having at least 80% sequence identity to SEQ ID NO:11 , and an amylase having at least 80% sequence identity to SEQ ID NO:5; p. A cellulase having at least 80% sequence identity to SEQ ID NO:9 or SEQ ID NO: 12 and a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8. q. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6 or SEQ ID NO: 7, a lipase having at least 80% sequence identity to SEQ ID NO:4, and an amylase having at least 80% sequence identity to SEQ ID NO:5; r. A protease having at least 80% sequence identity to any of SEQ I D NO: 1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a lipase having at least 80% sequence identity to SEQ ID NO:4, and an amylase having at least 80% sequence identity to SEQ ID NO:5; and s. A protease having at least 80% sequence identity to any of SEQ I D NO: 1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a lipase having at least 80% sequence identity to SEQ ID NO:4, a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8, and an amylase having at least 80% sequence identity to SEQ ID NO:5.
7. A composition according to claim 6, wherein the composition is selected from the group consisting of a. A cellulase having at least 80% sequence identity to SEQ ID NO:9 and a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8; b. A protease having at least 80% sequence identity to any of SEQ I D NO: 1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6, a mannanase having at least 80% sequence identity to SEQ ID NO: 7, and an amylase having at least 80% sequence identity to SEQ ID NO:5: c. A protease having at least 80% sequence identity to any of SEQ I D NO: 1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6, a mannanase having at least 80% sequence identity to SEQ ID NO: 7, a pectinase having at least 80% sequence identity to SEQ ID NO: 10, a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8 and an amylase having at least 80% sequence identity to SEQ ID NO:5; d. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6, a mannanase having at least 80% sequence identity to SEQ ID NO: 7, a pectinase having at least 80% sequence identity to SEQ ID NO: 10, a lipase having at least 80% sequence identity to SEQ ID NO:4 and an amylase having at least 80% sequence identity to SEQ ID NO:5; e. A protease having at least 80% sequence identity to any of SEQ I D NO: 1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6, a mannanase having at least 80% sequence identity to SEQ ID NO: 7, a lipase having at least 80% sequence identity to SEQ ID NO:4, a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8 and an amylase having at least 80% sequence identity to SEQ ID NO:5; f. A protease having at least 80% sequence identity to any of SEQ I D NO: 1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6, a mannanase having at least 80% sequence identity to SEQ ID NO: 7, a lipase having at least 80% sequence identity to SEQ ID NO:4, a pectinase having at least 80% sequence identity to SEQ ID NO: 10, a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8, a cellulase having at least 80% sequence identity to SEQ ID NO:9, and an amylase having at least 80% sequence identity to SEQ ID NO:5; g. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6, a mannanase having at least 80% sequence identity to SEQ ID NO: 7, a lipase having at least 80% sequence identity to SEQ ID NO:4, a pectinase having at least 80% sequence identity to SEQ ID NO: 10, a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8, a cellulase having at least 80% sequence identity to SEQ ID NO:9, a DNase having at least 80% sequence identity to SEQ ID NO:11 , and an amylase having at least 80% sequence identity to SEQ ID NO:5; h. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6, a mannanase having at least 80% sequence identity to SEQ ID NO: 7, a lipase having at least 80% sequence identity to SEQ ID NO:4, a pectinase having at least 80% sequence identity to SEQ ID NO: 10, a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8, a DNase having at least 80% sequence identity to SEQ ID NO:11 , and an amylase having at least 80% sequence identity to SEQ ID NO:5; i. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6, a mannanase having at least 80% sequence identity to SEQ ID NO: 7, a pectinase having at least 80% sequence identity to SEQ ID NO: 10, a xyloglucanase having at least 80% sequence identity to SEQ ID NO:8, a cellulase having at least 80% sequence identity to SEQ ID NO:9, a DNase having at least 80% sequence identity to SEQ ID NO:11 , and an amylase having at least 80% sequence identity to SEQ ID NO:5; and j. A protease having at least 80% sequence identity to any of SEQ ID NO:1 , or SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, or SEQ ID NO:14, a mannanase having at least 80% sequence identity to SEQ ID NO:6, a mannanase having at least 80% sequence identity to SEQ ID NO: 7, a lipase having at least 80% sequence identity to SEQ ID NO:4, and an amylase having at least 80% sequence identity to SEQ ID NO:5.
8. A detergent composition comprising the enzyme composition according to any of claims 5 to 7 and at least one surfactant.
9. The detergent composition according to any of claims 1 to 3 or claim 8 comprising 0.05- 20 wt% rhamnolipid, such as 1-15 wt% rhamnolipid, such as 2-10 wt% rhamnolipid, such as 3-9 wt% rhamnolipid, such as 4-8 wt% rhamnolipid.
10. The detergent composition according to claim 9, wherein at least 90 wt% of the rhamnolipid is mono rhamnolipid.
11 . The detergent composition according to any of claims 1 to 3 or 8 to 10, further comprising one or more additional enzymes selected from the group consisting of proteases, amylases, deoxyribonucleases, xyloglucanases, pectinases, pectin lyases, xanthan lyases, xanthan endoglucanases, peroxidases, haloperoxygenases, cellulases, licheninase, lipases, cutinases, catalases, oxidase, arabinose, galactanase and mannanases.
12. A method for removal of a stain on a textile during a wash cycle comprising contacting the textile with the detergent composition of any of claims 1 to 3 or 8 to 11 , wherein the temperature of the wash liquor is in the range of 5°C to 90°C, or in the range of 10°C to 80°C, or in the range of 10°C to 70°C, or in the range of 10°C to 60°C, or in the range of 10°C to 50°C, or in the range of 15°C to 40°C, or in the range of 20°C to 30°C.
13. A washing method for textile comprising: a. Exposing a textile to a wash liquor, said wash liquor comprising detergent composition according to any of claims 1 to 3 or 8 to 11 ; b. completing at least one wash cycle, and c. optionally rinsing the textile.
14. The washing method according to claim 13, wherein the temperature of the wash liquor is in the range of 5°C to 90°C, or in the range of 10°C to 80°C, or in the range of 10°C to 70°C, or in the range of 10°C to 60°C, or in the range of 10°C to 50°C, or in the range of 15°C to 40°C, or in the range of 20°C to 30°C.
15. Use of the detergent composition according to any of claims 1 to 3 or 8 to 11 for removal of a stain on textile in a wash liquor, wherein the temperature of the wash liquor is in the range of 5°C to 90°C, or in the range of 10°C to 80°C, or in the range of 10°C to 70°C, or in the range of 10°C to 60°C, or in the range of 10°C to 50°C, or in the range of 15°C to 40°C, or in the range of 20°C to 30°C.
PCT/EP2025/070123 2024-07-17 2025-07-14 Compositions comprising combination of enzymes Pending WO2026017636A1 (en)

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