CN107509735A - Synergistic mite killing composition and application thereof - Google Patents

Abstract

The invention discloses a synergistic acaricidal composition. The synergistic acaricidal composition is composed of asvermectin and pyrenzafen; 50:1. The present invention also provides an acaricide prepared by using the above-mentioned synergistic acaricidal composition, and the acaricide contains the synergistic acaricidal composition with a mass percentage ≥ 0.1%. The present invention also simultaneously provides the application of the above-mentioned synergistic acaricidal composition: preventing and controlling agricultural pest mites.

Description

Synergistic acaricidal composition and its application

technical field

The invention relates to an acaricidal composition, in particular to a synergistic acaricidal composition containing asvermectin and pyrazafen and its application.

Background technique

Tenvermectin (tenvermectin) is a new type of insecticidal and acaricidal compounds of biological origin, which belongs to macrolides, and has the characteristics of broad spectrum, high efficiency, low toxicity, small dose, and safe use. Asvermectin has a good control effect on Lepidoptera pests on fruit trees and vegetables, such as leafminer, diamondback moth, cotton bollworm and mites such as spider mites and panonychia.

Cyenopymfen (cyenopymfen) is a new type of pyrazole acaricide developed by Nissan Chemical Company. Its mechanism of action is novel. Enzymes, inhibit the normal function of mitochondria, to achieve the role of prevention and treatment. It has no cross-resistance with the acaricides used today, and can effectively control various pest mites on fruits, citrus, tea, and vegetables.

At present, some old domestic acaricide varieties (such as dicofol and dimeform, etc.) have been banned from use due to their safety and resistance problems, while acaricides with relatively high safety and better control effect (such as pyrimidine Varieties such as acaridamine, pyridaben and tetrafenazin) have also become increasingly prominent due to long-term use. Although new acaricides have been developed and produced in recent years (such as spirodiclofen, cyflufenofen, etc.), since mites are easily resistant to pests, long-term use of a single acaricide species is likely to cause The rapid development of resistance requires the alternating use of acaricides with different mechanisms of action. As acaricides with novel mechanism of action and biological acaricides with excellent activity, pyrifen and asvermectin can provide new options for the alternate use of acaricide varieties.

Contents of the invention

The technical problem to be solved in the present invention is to provide a novel low-toxicity, high-efficiency and long-lasting effect, delaying the generation of harmful mite drug resistance, can effectively prevent and treat agricultural Acaricidal composition for resistant pest mites).

In order to solve the above technical problems, the present invention provides a synergistic acaricidal composition, the synergistic acaricidal composition is composed of asvermectin and pyrenzafen; 1:50 to 50:1.

As an improvement of the synergistic acaricidal composition of the present invention: the mass ratio of asvermectin to pyraclofen is 1:20-20:1.

As a further improvement of the synergistic acaricidal composition of the present invention: the mass ratio of asvermectin to pyrifen is 1:10-5:1; more preferably: 0.1-5:1.

The present invention also simultaneously provides an acaricide prepared from the synergistic acaricidal composition, the acaricide contains the synergistic acaricidal composition with a mass percentage ≥ 0.1%.

As an improvement of the acaricide of the present invention: the acaricide contains a synergistic acaricide composition with a mass percentage of 1% to 80%.

As a further improvement of the acaricide of the present invention: the acaricide is emulsifiable concentrate, suspension concentrate, microemulsion, water emulsion, wettable powder, water dispersible granule, dispersible oil suspension concentrate.

The present invention also simultaneously provides the application of the above-mentioned synergistic acaricidal composition: preventing and controlling agricultural pest mites.

As an improvement in the use of the synergistic acaricidal composition of the present invention: it is used to prevent and control harmful mites that have developed resistance to propargite and abamectin.

The acaricidal composition of the present invention contains two active ingredients, one active ingredient is asvermectin, and the other active ingredient is pyrimectin. The present invention combines the two, and can more effectively prevent and control the harm of resistant pest mites. It can reduce the amount of pesticides, significantly improve the acaricidal effect, reduce environmental pollution, solve the problem that harmful mites are prone to resistance to a single agent, and prolong the service life of the agent. It is an important means of comprehensive pest management. There is no report and application of the mixture of asvermectin and pyrazafen to prevent and control agricultural pest mites.

The acaricide provided by the present invention, in addition to the above-mentioned composition, may further include pesticide-acceptable carriers and adjuvants usually used for preparing insecticidal and acaricidal compositions. The carrier can be solid or liquid.

Suitable solid carriers include: minerals, plants, synthetic fillers and inorganic salts. The minerals include silicates, carbonates, sulfates and oxides. Silicates such as kaolin, sepiolite, nacrite, montmorillonite, mica, vermiculite, pyrophyllite, talc. Carbonates such as: calcium carbonate, dolomite. Sulfates such as ammonium sulfate, sodium sulfate, calcium sulfate. Oxides such as quicklime, magnesium lime, diatomaceous earth. Vegetables such as: citrus residue, corn cob, chaff powder, rice husk, soybean straw powder, tobacco powder, walnut shell, sawdust. Synthetic fillers such as precipitated calcium carbonate hydrate, precipitated calcium carbonate, white carbon black. Inorganic salts such as potassium chloride, sodium chloride.

Liquid carriers include water and organic solvents. When the active ingredient is water emulsion or suspoemulsion, the organic solvent plays the role of solubilization and antifreeze. Suitable organic solvents include aromatics such as benzene, xylene, toluene, alkylbenzenes, alkylnaphthalenes, and chlorinated aromatics; chlorinated aliphatics such as vinyl chloride, chloroform, methylene chloride, chloroform, carbon tetrachloride and polychloroethanes; aliphatic hydrocarbons such as petroleum distillates, cyclohexane, light mineral oils and paraffins. Alcohols such as methanol, ethanol, isopropanol, butanol, isobutanol, ethylene glycol, propylene glycol, glycerol, fatty alcohols, etc.; ethers such as methyl glycol ether, ethyl glycol ether, petroleum ether. Ketones such as acetone, methyl ethyl ketone, methyl isobutyl ketone, cyclohexanone and isophorone, N-methyl-pyrrolidone; special solvents also include dimethylformamide, dimethyl sulfoxide, polyethylene glycol , has been nitrile; oil phase carrier such as vegetable oil and methylated vegetable oil. The above-mentioned carriers may be used alone, or in combination, or in combination with water.

The auxiliary agent of the present invention may include one or more of surfactants, defoamers, thickeners, suspending agents and antifreezing agents as required, and may also include other auxiliary agents commonly used in this industry as required.

Surfactants can be emulsifiers, dispersants or wetting agents; can be ionic or nonionic. Suitable surfactants include: sodium and calcium salts of polyacrylic acid and lignosulfonic acid; polycondensation products of fatty acids or fatty amines having at least 12 carbon atoms in the molecule with ethylene oxide and/or propylene oxide; glycerol, Fatty acid esters of 1-lauryl alcohol, 1-myristyl alcohol, sorbitol, sucrose or pentaerythritol; and their polycondensation products with ethylene oxide and/or propylene oxide; sulfate salts or Sulfonates; alkali or alkaline earth metal salts, preferably sodium, of sulfuric or sulfonic acids containing at least 10 carbon atoms in the molecule, such as sodium lauryl sulfate, sodium secondary alkyl sulfate, ricinium sulfonate Sodium salt of sesame oil, sodium alkylarylsulfonate and sodium dodecylbenzenesulfonate; polymer of ethylene oxide, copolymer of ethylene oxide and propylene oxide.

Emulsifiers include nonionic emulsifiers and commercially available emulsifiers. Nonionic emulsifiers are preferably polyoxyethylene fatty acid esters, polyoxyethylene fatty alcohol ethers and polyoxyethylene fatty ammonia, such as ethoxylated castor oil, polyoxyethylene (n ₂₀ ) phenethylphenol ether oleate, Alkylaryl polyoxyethylene polyoxypropylene ether, alkylaryl formaldehyde resin polyoxyethylene ether, tristyrylphenol polyoxyethylene (n ₂₀ ), ether phosphorylated triethanolamine salt, diphenylethylphenol polyoxyethylene Oxyethylene ether (n ₁₈ ) formaldehyde condensate, diphenylphenol polyoxyethylene (n ₁₈ ) polyoxypropylene ether (n ₂₀ ), etc. Commercially available emulsifiers include: Agricultural Milk 0201B, Agricultural Milk 0203B, Agricultural Milk 100 ^# , Agricultural Milk 500 ^# , Agricultural Milk 600 ^# , Agricultural Milk 600-2 ^# , Agricultural Milk 1601, Agricultural Milk 2201, Agricultural Milk NP-10 , Nongru NP-15, Nongru 507 ^# , Nongru OX-635, Nongru OX-622, Nongru OX-653, Nongru OX-667, Nongru CS-7, Ningru 36 ^# .

The dispersant of the present invention includes: sodium lignosulfonate, pull-open powder, calcium lignosulfonate, methylnaphthalenesulfonic acid formaldehyde condensate, naphthalenesulfonic acid formaldehyde condensate, sodium methylenenaphthalenesulfonate, oil Sodium acid methyl amino ethyl sulfonate, epoxy polyether, p-tert-butyl ether, dibutyl naphthalene sulfonic acid formaldehyde condensate, phosphates such as sodium hexametaphosphate, alkylphenol polyoxyethylene phosphate, alkanes Alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, N-methyl-fatty acyl-taurine sodium alkylphenol polyoxyethylene ether formaldehyde condensate, aralkylphenol polyoxyethylene ether phosphate ester alkylphenol poly Oxyethylene ether, castor oil ethylene oxide adduct, ethylene oxide-propylene oxide block copolymer alkylphenol polyoxyethylene ether formaldehyde condensate, alkylphenol polyoxyethylene polyoxypropylene ether and methyl cellulose.

The wetting agent described in the present invention includes: sodium lauryl sulfate, sodium alkyl alcohol polyoxyethylene ether sulfate, octylphenol polyoxyethylene ether sulfate, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate , Sodium Alkyl Sulfonate, Sodium Dodecyl Benzene Sulfonate, Sodium Alkyl Naphthalene Sulfonate, Alkyl Succinate Sulfonate, Sodium Lauryl Polyoxyethylene Ether Sulfonate, Sodium Alkylamide Taurate , fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene ether, polyoxyethylene polyoxypropylene block polymer, sorbitan fatty acid ester polyoxyethylene ether, etc.

The thickener of the present invention can be artificially synthesized, such as carboxymethyl alcohol, polyvinyl alcohol or polyvinyl acetate; it can also be a natural water-soluble polymer, such as xanthan gum, gelatin, gum arabic , polyvinylpyrrolidone, aluminum magnesium silicate, polyvinyl alcohol, polyethylene glycol, phenolic resin, shellac, carboxymethyl cellulose and sodium alginate, etc.

The defoaming agent described in the present invention is preferably: foam enemy, silicones, C _{8 ~ 10} fatty alcohols, C _{10 ~ 20} saturated fatty acids and amides, etc.

The acaricidal composition of the present invention can be formulated into emulsifiable concentrates, suspension concentrates, microemulsions, water emulsions, wettable powders, water dispersible granules, dispersible oil suspension concentrates and the like. When formulated into preparations, the emulsifiable concentrates, suspensions, microemulsions, water emulsions, wettable powders, water dispersible granules, and dispersible oil suspensions contain more than 0.1% by mass of the acaricidal composition (Following percentages are mass percentages).

When it is a wettable powder, the active ingredient is 0.1%-80%, the wetting agent is 2%-10%, the dispersant is 5%-10%, and the filler is 8-90%.

When it is an emulsifiable concentrate, the active ingredient is 0.1% to 60%, the emulsifier is 1% to 10%, and the solvent is added to 100%.

When it is a suspending agent, the active ingredient is 0.1%-60%, the wetting agent is 1%-5%, the dispersant is 1%-8%, the thickener is 0.1%-1%, the antifreeze agent is 2%-5%, and water is added to 100%.

When it is a microemulsion, the active ingredient is 0.1%~40%, the emulsifier is 1%~12%, the cosolvent is 0%~10%, the stabilizer is 0%~5%, the solvent is 0%~40%, and the water is made up to 100%. .

When it is water emulsion, active ingredient 0.1%~40%, emulsifier 1%~20%, cosolvent 0%~15%, stabilizer 0%~5%, solvent 10%~40%, water to 100% .

For water dispersible granules, active ingredient 0.1%-80%, surfactant 0%-2%, wetting agent 1%-15%, dispersant 1%-15%, disintegrant 1%-10%, Stabilizer 0% ~ 5%, filling up to 100%.

When it is a dispersible oil suspension concentrate, the active ingredient is 0.1% to 60%, the wetting agent is 1% to 5%, the dispersant is 1% to 8%, the thickener is 0.1% to 1%, and the antifreeze agent is 1% to 3%. The oil phase carrier is made up to 100%.

The agricultural acaricidal composition of the present invention can be directly made into a finished preparation form, that is, each material in the composition has been mixed, and is directly used for acaricide; ) mixed directly. The concentrates of the present invention are generally mixed with water to obtain the desired concentration of active substance.

The present invention also includes the use of the composition of the present invention prepared as an acaricidal preparation to control agricultural and forestry pests. Specifically suitable for the control of a variety of important agricultural pests such as cotton spider mites, vegetable spider mites, hawthorn spider mites, citrus panonychia, citrus rust mite, apple panonychia, apple spider mites, two-spotted spider mites, gall mites , Acacia mites, etc. For fruit trees such as apples, pears, citrus, litchi, etc., cereals such as wheat, rice, etc., beans such as soybeans, kidney beans, etc., cotton, tea trees, vegetables such as cabbage, cauliflower, cabbage, rape, tomato, pepper, flowers such as rose etc.

The application site of the agricultural acaricide described in the present invention is farmland, orchard or warehouse.

The agricultural acaricidal composition of the present invention can be applied by common methods, such as pouring, spraying and spraying. The application rate varies with climatic conditions or crop status (about 450-750L/ha), and the application time can be before or after the pest mite damage. The duration of the protective effect is usually related to the content of the individual compounds in the composition, but also to external factors, such as climate, which can be mitigated by using appropriate formulations.

The agricultural acaricidal composition of the present invention can also be used in combination with other compounds with insecticidal, herbicidal and bactericidal properties, and can also be used in combination with nematicides, protective agents, growth regulators, plant nutrients or soil regulators.

Compared with the prior art, the composition containing two active components provided by the invention has the following advantages:

(1) The combination of the two active ingredients in the composition shows a strong synergistic effect on the harmful mite Tetranychus cinnabarinus within a certain ratio range, and the acaricidal effect of the mixed composition is more obvious than that of its single agent improvement;

(2) The combination of the two active ingredients in the composition shows obvious quick-acting and long-lasting effects on harmful mites such as citrus Panonychus citri and apple Panonychus ulmi within a certain ratio range higher than a single dose;

(3) The composition is composed of active ingredients with different action mechanisms, and the number of action sites is increased, which is beneficial to overcome and delay the generation of insecticide resistance of pest mites, and prolong the service life of pesticides. The composition preparation is used to prevent and control panonychia citrus which has developed resistance to commonly used agents such as propargite and avermectin, and has excellent control effect.

detailed description

The present invention will be further described below in conjunction with specific examples, but the present invention is not limited to these specific implementations. Those skilled in the art will realize that the present invention covers all alternatives, modifications and equivalents as may be included within the scope of the claims.

1. Indoor bioactivity assay

In order to understand the toxicity of asvermectin, pyrazafen and their combination to harmful mites, an indoor bioactivity test was carried out. The test refers to the Indoor Bioassay Test Guidelines for Pesticides and Pesticides (Agricultural Industry Standard NY/T1154.7-2006) and the SOP for the Evaluation of Bioactivity of Pesticides (Pesticide Volume), using the spray method. The test target is Tetranychuscinnabarinus. Cut off the leaves with stalks, insert them into a glass bottle with water, pick up a certain number of adult mites from Tetranychus cinnabarinus, and remove the adult mites after the adult mites lay eggs for 24 hours. After the eggs are all hatched for 48 hours, they are sprayed and dried naturally. Place them in an observation room (25±2°C, humidity 70%-80%, 16h light/d) for cultivation. The experiment was repeated 3 times, and a blank control was set up, and the investigation was carried out after 48 hours. During the investigation, the insects that could not crawl normally were regarded as dead insects. The survey data was analyzed and counted with the professional version of "DPS Data Processing System" V14.50, and the regression line and LC50 value were obtained. The virulence index was calculated by Sun Yunpei's method (Sun et al., 1960), and the co-toxicity coefficient was calculated (Table 1).

Theoretical mixed toxicity index (TTI) = Toxicity index of A × A content in mixed use (%)

Toxicity index of +B × B content in the mixture (%)

If the co-toxicity coefficient is greater than 120, it indicates a synergistic effect; if it is less than 80, it indicates an antagonistic effect; if it is greater than 80 but less than 120, it indicates an additive effect.

Table 1. Indoor toxicity test results of asvermectin and pyrifenin and their mixtures against Tetranychus cinnabarinus

potion _LC50 (mg/L) measured toxicity index theoretical virulence index co-toxicity coefficient Asvermectin (A) 0.0212 3920.28 - - Cyclopyrafen (B) 0.8311 100.00 - - A:B(1:100) 0.4920 168.92 137.82 122.56 A:B(1:50) 0.3450 240.90 174.91 137.73 A:B(1:20) 0.1881 441.84 281.92 156.73 A:B(1:10) 0.1105 752.13 447.30 168.15 A:B(1:5) 0.0662 1255.44 736.71 170.41 A:B(1:1) 0.0231 3597.84 2010.14 178.98 A:B(5:1) 0.0143 5811.89 3283.57 177.00 A:B(10:1) 0.0147 5653.74 3572.98 158.24 A:B(20:1) 0.0135 6156.30 3738.36 164.68 A:B(50:1) 0.0151 5503.97 3845.38 143.13 A:B(100:1) 0.0162 5130.25 3882.46 132.14

The results of indoor toxicity test showed that the mixture of tianvermectin and pyrazafen had a synergistic effect on Tetranychus cinnabarinus in the range of 1:100-100:1 in mass ratio. The synergistic effect is obvious in the range of 1:20-20:1, and the most significant synergistic effect is in the range of mass ratio of 1:10-5:1. It is scientifically reasonable to mix asvermectin and pyrimectin to control the above pests.

2. Preparation of the composition

The percentages in the proportioning ratio of all preparations in the invention are percentages by mass.

One), the preparation of emulsifiable oil:

According to the formula requirements, add the solvent, the original drug and the emulsifier respectively, mix evenly, and if necessary, heat and dissolve in a water bath to obtain a transparent emulsifiable oil.

Example 1 10% emulsifiable concentrate

Asvermectin 5%, fenpyrafen 5%, Nongru 0203B 6%, Nongru 0201B 9%, cyclohexanone 8%, mineral spirits 150 to make up to 100%.

Example 2 20% emulsifiable concentrate

15% asvermectin, 5% pyraclofen, 6% Nongru 2201#, 8% Nongru 0201B, 8% cyclohexanone, and 150 solvent naphtha to make up to 100%.

Two), the preparation of wettable powder

According to the requirements of the formula, the original drug, various additives and fillers are fully mixed, and the processed product is obtained after being pulverized by an ultra-fine pulverizer.

Embodiment 3 60% wettable powder

30% asvermectin, 30% pyrimectin, 1.8% sodium dodecylbenzenesulfonate, 2% p-tert-butyl ether, 10% white carbon black, 10% sodium lignosulfonate, light carbonic acid Calcium supplement to 100%.

Embodiment 4 60% wettable powder

50% asvermectin, 10% pyrimectin, 2% sodium dodecylbenzenesulfonate, 2% p-tert-butyl ether, 5% white carbon black, 10% sodium lignosulfonate, light carbonic acid Calcium supplement to 100%.

Three), the preparation of water dispersible granules

Mix and pulverize the original drug, powdery carrier, wetting and spreading agent, binder, etc., add water and knead, then add to a granulator equipped with a certain size screen for granulation. Then dry and sieve (according to the range of the sieve) to obtain granular products.

Example 5 40% water dispersible granules

10% asvermectin, 30% pyraclofen, 8% sodium N-methyl-oleoyl-taurine, 10% naphtholsulfonic acid formaldehyde condensate, 8% soluble starch, 10% sodium sulfate, kaolin Top up to 100%.

Example 6 50% water dispersible granules

25% asvermectin, 25% pyraclofen, 15% sodium lignin sulfonate, 5% sodium methylnaphthalene sulfonate formaldehyde condensate, 5% epoxy polyether, 10% bentonite, kaolin to 100% .

Four), the preparation of suspending agent

According to the requirements of the formula, use water as the medium, mix the original drug, dispersant, wetting agent, suspending agent and antifreeze evenly, then add it to the sand mill, perform sand grinding to a certain particle size and filter, then add the thickener Shear and mix well.

Example 7 30% suspension concentrate

5% asvermectin, 25% pyrimectin, 5% sodium lignosulfonate, 0.3% white carbon black, 2.0% EFW (a mixture of alkylnaphthalene sulfonate and anionic wetting agent), dihydrogen phosphate Potassium 0.2%, ethylene glycol 4%, tributyl phosphate 0.2%, water to make up to 100%.

Example 8 55% suspension concentrate

50% Orbactin, 5% Betrafenflux, 2.5% Sodium Methyl Naphthalene Sulfonate Formaldehyde Condensate, 2.0% EFW (a mixture of alkylnaphthalene sulfonate and anionic wetting agent), Nongru OX-656 3.0%, glycerol 4%, tributyl phosphate 0.2%, water to make up to 100%.

Five), the preparation of emulsion in water

Mix and stir the original drug, solvent and emulsifier evenly to form a uniform oil phase, mix and stir water and ethylene glycol to form a water phase, and slowly add the oil phase to the water phase under high shear conditions, that is Emulsions in water are available.

Embodiment 9 15% water emulsion

5% asvermectin, 10% pyraclofen, 30% solvent naphtha S-150, 3% tristyrylphenol polyoxyethylene ether phosphate, styrylphenol formaldehyde resin polyoxyethylene polyoxypropylene ether 5 %, propylene glycol 3%, water to make up to 100%.

Embodiment 10 25% water emulsion

Tenvermectin 15%, fenpyrafen 10%, solvent naphtha S-150 30%, tristyrylphenol polyoxyethylene ether phosphate 4%, hydroxyl-terminated polyoxyethylene polyoxypropylene ether block copolymer 5 %, ethylene glycol 3%, water to make up to 100%.

Six), the preparation of microemulsion

Mix and stir the original drug, solvent, and emulsifier evenly to form a uniform oil phase, gradually add water to the oil phase, and stir until uniform and transparent.

Embodiment 11 8% microemulsion

2% asvermectin, 6% pyraclofen, 10% isopropanol, 15% mineral spirits S-150, 2% calcium dodecylbenzenesulfonate, 18% styrylphenyl polyoxyethylene ether , water up to 100%.

Example 12 15% microemulsion

5% asvermectin, 10% pyrimectin, 6% N-octylpyrrolidone, 15% solvent naphtha S-150, 2% calcium dodecylbenzenesulfonate, polyoxyethylene ether of alkylphenol formaldehyde resin 15%, water up to 100%.

Seven), the preparation of dispersible oil suspension

After mixing and stirring the original drug, dispersant, emulsifier, thickener and oil phase carrier, add it to a sand mill and grind it to a certain particle size.

Example 13 10% dispersible oil suspension concentrate

5% asvermectin, 5% pyraclofen, 2% alkylphenol polyoxyethylene ether phosphate, 13% castor oil polyoxyethylene ether, 2% organic bentonite, and 100% methyl oleate.

Example 14 30% dispersible oil suspension concentrate

10% asvermectin, 20% pyraclofen, 3% tristyrylphenol polyoxyethylene ether phosphate, 14% fatty alcohol polyoxyethylene ether, 1.5% organic bentonite, and make up to 100% with methyl oleate %.

3. Drug efficacy test in field plots

Test 1. Field drug efficacy test on citrus whole claw mite (Panonychus citri)

The control effect against Panonychus citrus was determined under field conditions. The test was carried out in accordance with the provisions of "Pesticide Field Efficacy Test Guidelines (1) - Acaricides Control Panonychus citrus GB/T 17980.11-2000", and citrus leaves in each test plot were selected according to the east, south and west. , North 5 azimuth marks, investigate 2 plants in each plot, and investigate the number of live insects on each plant. Investigate the immediate effect of the drug 1 day after the drug, and investigate the persistent effect of the drug 7 days, 14 days, and 21 days after the drug. The application rate is 750L/ha.

Test results: The control effects of the composition preparations with different ratios on Panonychus citrus were better than those of pyrifenate and asvermectin alone. In terms of quick effect, one day after the drug, the control effect of the composition in the range of 1:10 to 5:1 is 76.8% to 80.1%, while the control effect of other compositions is 71.5% to 72.2%. The control effects of single agent were 67.2% and 62.8%. In terms of long-lasting effect, the long-lasting effect of the composition in the ratio of 1:10 to 5:1 is 14 to 21 days, and the control effect is 93.8% to 95.0% after 21 days of medicine, while the two single doses and The persistence period of other proportioning compositions is 7-14 days, and the control effects after 14 days are all lower than 83.1%-88.3%, see Table 2 for details.

The results showed that, when the ratio of the composition was within the range of 1:10 to 5:1, the quick-acting and persistent effects of the agent against Panonychus citrus were significantly higher than those of the two single-dose and other ratio compositions.

Table 2. Field efficacy test results for Panonychus citrus

Remarks: Change the mass proportioning relationship between asvermectin and fenpyrafen (as described in Table 2), and follow the corresponding preparation methods of the suspensions in the examples to obtain the suspensions as described in Table 2 .

Test 2. Field efficacy test on apple claw mite (Panonychus ulmi)

The control effect against Panonychus apple was determined under field conditions. The test was carried out in accordance with the provisions of "Guidelines for Field Efficacy Tests of Pesticides (1) - Acaricides Control Apple Tetranychus GB/T 17980.7-2000". The apple leaves of each test plot were marked with 5 fixed points according to the five orientations of east, south, west and north, and 2 plants were investigated in each plot, and the number of live insects on each plant was investigated. Investigate the immediate effect of the drug 1 day after the drug, and investigate the persistent effect of the drug 7 days, 14 days, and 21 days after the drug. The application rate is 750L/ha.

Test results: The control effects of the composition preparations with different ratios on apple claw mites are better than those of pyrifen and asvermectin alone. In terms of quick effect, one day after the drug, the control effect of the composition in the range of 1:10 to 5:1 is 75.9% to 82.9%, while the control effect of other compositions is 71.7% to 72.1%. The control effects of single agent were 66.3% and 63.5%. In terms of persistent effect, the duration of the composition in the ratio of 1:10 to 5:1 is 14 to 21 days, and the control effect is 93.1% to 94.4% after 21 days of administration, while two single doses and other The duration of the medicine of the proportioning composition is 7-14 days, and the control effect is 83.6%-89.6% 14 days after the medicine. See Table 3 for details.

The results showed that, when the ratio of the composition was within the range of 1:10 to 5:1, the quick-acting and persistent effects of the agent against Panonychus apple were significantly higher than those of the two single-dose and other ratio compositions.

Table 3 field efficacy test results on apple panonychia

Experiment 3. Field efficacy test of Panonychus citrus mites that have developed resistance to both propargite and abamectin

The composition preparation was used as the test agent, and Panonychus citrus, which had developed resistance to propargite and abamectin, was selected as the control object. Commonly used drugs such as propargite and avermectin were used as control drugs. The test method and survey method are the same as test 1.

Test results: The control effect of the composition preparation on Panonychus citrus in the range of 1:10-5:1 is higher than that in other ranges, and the control effect on 1 day, 7 days, 14 days, and 21 days after treatment They are 76.0%-81.7%, 92.1%-94.3%, 94.2%-98.7%, 91.8%-94.1%, which are far higher than the super-large doses of common citrus panonychia control agents such as propargite and abamectin. The next anti-efficacy. The results show that the composition preparation has excellent control effect on controlling Panonychus citrus which has developed resistance to commonly used agents such as propargite and abamectin. See Table 4 for details.

Table 4 Field efficacy test results of resistant Panonychus citrus

Remarks: Change the mass proportioning relationship between asvermectin and fenpyrafen (as described in Table 4), and according to the preparation method of the corresponding composition preparation in the above examples, the test agent as described in Table 4 can be obtained .

The conventional effective concentration of propargite used in the field is about 250mg/L, and the conventional effective concentration of avermectin used in the field is about 10mg/L. Therefore, the above-mentioned Table 4 has been used in a large amount.

The above data shows that the combination of asvermectin and pyraclofen has obvious synergistic effect on the prevention and treatment of Panonychus citrus and Panonychus apple under a specific proportioning range. Efficacy) is better than the single use of asvermectin and pyrimectin.

The above-mentioned indoor detection and field drug efficacy test results show that the beneficial effects produced by the acaricidal composition of the present invention are:

(1) Compared with a single agent, the composition has a significant synergistic effect on harmful mites, and obviously improves the quick-acting and persistent effects on harmful mites;

(2) After the long-lasting effect is improved, the frequency of medication can be reduced, the cost of medication can be reduced, and environmental pollution and pesticide residues can be reduced;

(3) The composition is composed of active ingredients with different action mechanisms, and the number of action sites is increased, which is conducive to overcoming and delaying the generation of pest resistance. Specific composition preparations are used to prevent and control common agents such as propargite and avermectin The resistant Panonychus citrus has excellent control effect. The composition of the invention has no obvious adverse effects on fruit trees, the leaf color and growth are normal, the safety is good, and the safety requirements of pesticide preparations are met.

Finally, it should be noted that the above examples are only some specific embodiments of the present invention. Obviously, the present invention is not limited to the above embodiments, and many variations are possible. All deformations that can be directly derived or associated by those skilled in the art from the content disclosed in the present invention should be considered as the protection scope of the present invention.

Claims (8)

1. The synergistic acaricidal composition is characterized in that: the synergistic acaricidal composition is made up of asvermectin and pyrenzafen; the mass ratio of asvermectin to pyrenzafen is 1:50 ~50:1. 2 . The synergistic acaricidal composition according to claim 1 , characterized in that: the mass ratio of asvermectin to bezafenid is 1:20-20:1. 3 . The synergistic acaricidal composition according to claim 2 , characterized in that: the mass ratio of asvermectin to bezafenid is 1:10-5:1. 4. The acaricide prepared by using the synergistic acaricidal composition according to any one of claims 1 to 3, characterized in that: the acaricide contains a synergistic acaricidal composition with a mass percentage ≥ 0.1% . 5. The acaricide according to claim 4, characterized in that: the acaricide contains a synergistic acaricide composition with a mass percentage of 1%-80%. 6. The acaricide according to claim 5, characterized in that: the acaricide is emulsifiable concentrate, suspension concentrate, microemulsion, water emulsion, wettable powder, water dispersible granule, or dispersible oil suspension concentrate. 7. The use of the synergistic acaricidal composition according to any one of claims 1 to 3, characterized in that: preventing and controlling agricultural pest mites. 8. The use of the synergistic acaricidal composition according to claim 7, characterized in that: it is used to control harmful mites that have developed resistance to propargite and abamectin.