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CN106459885A - 表达阿维菌素类似物的重组微生物及其用途 - Google Patents

表达阿维菌素类似物的重组微生物及其用途 Download PDF

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Publication number
CN106459885A
CN106459885A CN201480077055.0A CN201480077055A CN106459885A CN 106459885 A CN106459885 A CN 106459885A CN 201480077055 A CN201480077055 A CN 201480077055A CN 106459885 A CN106459885 A CN 106459885A
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avm
streptomycete
genes
analog
activity
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CN106459885B (zh
Inventor
黄隽
王继栋
陈安良
邓爱文
林甲壇
余贞
李美红
李娜
王海彬
郑玲辉
白骅
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Shenzhen Tianwei biopharmaceutical Co.,Ltd.
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Zhejiang Hisun Pharmaceutical Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
    • C12P19/62—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
    • C12P19/623—Avermectin; Milbemycin; Ivermectin; C-076
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Saccharide Compounds (AREA)

Abstract

本发明涉及一种表达阿维菌素或其类似物的重组微生物及其构建方法。还涉及利用所述重组微生物生产阿维菌素或其类似物的方法，以及利用本发明方法获得的阿维菌素或其类似物。此外本发明还涉及所得阿维菌素或其类似物用作杀虫剂的用途。利用本发明的重组微生物生产阿维菌素或其类似物具有众多优点，例如至少包括以下一种：稳定性好、得率高，工艺简单，环境友好，极大地节省生产成本。

Description

PCT国内申请，说明书已公开。

Claims (12)

  1. PCT国内申请，权利要求书已公开。
CN201480077055.0A 2014-03-10 2014-04-28 表达阿维菌素类似物的重组微生物及其用途 Active CN106459885B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201410085431 2014-03-10
CN2014100854312 2014-03-10
PCT/CN2014/076372 WO2015135242A1 (zh) 2014-03-10 2014-04-28 表达阿维菌素类似物的重组微生物及其用途

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CN106459885A true CN106459885A (zh) 2017-02-22
CN106459885B CN106459885B (zh) 2019-07-23

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CN201480077055.0A Active CN106459885B (zh) 2014-03-10 2014-04-28 表达阿维菌素类似物的重组微生物及其用途
CN201580013257.3A Pending CN106795196A (zh) 2014-03-10 2015-03-10 十六元大环内酯类化合物及其应用

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US (1) US10334850B2 (zh)
EP (1) EP3118305B1 (zh)
JP (1) JP6346673B2 (zh)
CN (2) CN106459885B (zh)
AU (1) AU2014386509B2 (zh)
CA (1) CA2940016A1 (zh)
CL (1) CL2016002223A1 (zh)
EA (1) EA033293B1 (zh)
IL (1) IL247596B (zh)
MX (1) MX2016011367A (zh)
UA (1) UA116835C2 (zh)
WO (1) WO2015135242A1 (zh)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110305818A (zh) * 2019-08-15 2019-10-08 齐鲁制药（内蒙古）有限公司 一种阿维菌素菌种选育方法
CN110540944A (zh) * 2018-05-28 2019-12-06 浙江海正药业股份有限公司 一种天维菌素产生菌及其应用
CN114931144A (zh) * 2022-06-21 2022-08-23 台州科技职业学院 一种大环内酯类化合物及其在防治农林害虫和害螨中的应用

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* Cited by examiner, † Cited by third party
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WO2017052232A1 (ko) * 2015-09-22 2017-03-30 주식회사 팜한농 밀베마이신을 생산하는 재조합 미생물 및 이를 이용한 밀베마이신 생산
CN110832066B (zh) * 2017-03-13 2023-03-03 浙江海正药业股份有限公司 一种伊维菌素B1b产生菌及其应用
CN108660101B (zh) * 2017-04-01 2021-07-09 浙江海正药业股份有限公司 表达天维菌素b的重组微生物、制备方法及其用途
CN107494559B (zh) * 2017-08-29 2020-09-29 浙江农林大学 防治柑桔潜叶蛾的杀虫组合物及其用途
CN107509735B (zh) * 2017-08-29 2020-09-11 浙江农林大学 增效杀螨组合物及其用途
CN110540556B (zh) * 2018-05-28 2023-04-28 深圳市天维生物药业有限公司 一种分离和提纯天维菌素的方法
CN110540567B (zh) 2018-05-28 2024-07-30 深圳市天维生物药业有限公司 一种天维菌素b的晶型及其制备方法和用途

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DE4031039A1 (de) * 1989-10-03 1991-04-11 Ciba Geigy Ag 13(alpha)-zuckerderivate von milbemycinen, deren herstellung und verwendung gegen ekto- und endoparasiten am nutztier

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US4134973A (en) * 1977-04-11 1979-01-16 Merck & Co., Inc. Carbohydrate derivatives of milbemycin and processes therefor
EP0235085A1 (de) * 1986-02-20 1987-09-02 Ciba-Geigy Ag 13-Beta-Zuckerderivate von Milbemycinen, deren Herstellung und Verwendung gegen Ekto-und Endoparasiten am Nutztier oder an der Nutzpflanze
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JP2888586B2 (ja) * 1990-03-05 1999-05-10 社団法人北里研究所 エバーメクチンの特定成分を選択生産するための微生物およびその選択的製造法
US8796230B2 (en) * 2010-01-22 2014-08-05 University Of Southern California Ivermectin antagonizes ethanol inhibition in P2X4 receptors
CN103834605B (zh) * 2012-11-21 2018-09-21 中国科学院上海有机化学研究所 一种阿维菌素产生菌及其制备方法和应用
CN104910228B (zh) * 2014-03-10 2018-12-07 浙江农林大学 十六元大环内酯类化合物及其应用

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4031039A1 (de) * 1989-10-03 1991-04-11 Ciba Geigy Ag 13(alpha)-zuckerderivate von milbemycinen, deren herstellung und verwendung gegen ekto- und endoparasiten am nutztier

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
周海娇 等: "只产阿维菌素B组分的基因工程菌的构建", 《沈阳药科大学学报》 *
朱浩君 等: "Avermectin C5-O-甲基转移酶基因的克隆、序列分析及其基因置换", 《中国抗生素杂志》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110540944A (zh) * 2018-05-28 2019-12-06 浙江海正药业股份有限公司 一种天维菌素产生菌及其应用
CN110540944B (zh) * 2018-05-28 2022-08-02 深圳市天维生物药业有限公司 一种天维菌素产生菌及其应用
CN110305818A (zh) * 2019-08-15 2019-10-08 齐鲁制药（内蒙古）有限公司 一种阿维菌素菌种选育方法
CN114931144A (zh) * 2022-06-21 2022-08-23 台州科技职业学院 一种大环内酯类化合物及其在防治农林害虫和害螨中的应用

Also Published As

Publication number Publication date
CN106795196A (zh) 2017-05-31
EP3118305A4 (en) 2018-03-21
EA033293B1 (ru) 2019-09-30
EP3118305B1 (en) 2019-08-14
AU2014386509B2 (en) 2018-07-05
US20170013837A1 (en) 2017-01-19
EP3118305A1 (en) 2017-01-18
JP2017512465A (ja) 2017-05-25
IL247596A0 (en) 2016-11-30
EA201691754A1 (ru) 2016-12-30
AU2014386509A1 (en) 2016-09-22
CL2016002223A1 (es) 2017-04-07
CN106459885B (zh) 2019-07-23
JP6346673B2 (ja) 2018-06-20
IL247596B (en) 2019-05-30
US10334850B2 (en) 2019-07-02
UA116835C2 (uk) 2018-05-10
MX2016011367A (es) 2017-02-02
CA2940016A1 (en) 2015-09-17
WO2015135242A1 (zh) 2015-09-17

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Effective date of registration: 20210719

Address after: 518000 805, Changsheng building, 4014 Huaqiang North Road, Changcheng community, Yuanling street, Futian District, Shenzhen City, Guangdong Province

Patentee after: Shenzhen Tianwei biopharmaceutical Co.,Ltd.

Address before: Jiaojiang District of Taizhou City, Zhejiang province 318000 road outside No. 46

Patentee before: ZHEJIANG HISUN PHARMACEUTICAL Co.,Ltd.