CN106353490B - Test device for cell control decompression model - Google Patents
Test device for cell control decompression model Download PDFInfo
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- CN106353490B CN106353490B CN201610809076.8A CN201610809076A CN106353490B CN 106353490 B CN106353490 B CN 106353490B CN 201610809076 A CN201610809076 A CN 201610809076A CN 106353490 B CN106353490 B CN 106353490B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention provides a test device for a cell control decompression model, wherein a positioning ring foot is arranged at the central position of the bottom surface of a flexible slide glass, a positioning support seat is arranged at the central position of the bottom in a culture dish, and the positioning ring foot is sleeved on the positioning support seat; the top surface of flexible slide glass be provided with the atress locating hole around the position symmetry, the central point on the lid be provided with the screw sleeve that link up the lid, install the threaded rod in the screw sleeve, the threaded rod stretches into the inside one end of culture dish and rotates telescopic one end and link to each other, rotates the sleeve and can rotate for the threaded rod, rotates telescopic other end rigid coupling in the central point of support, support tip all around is provided with vertical pressurization post, the pressurization post top is used for the bending flexible slide glass in the pressurization locating hole. The flexible slide glass can be taken out from the test device, is convenient to directly observe under the microscope and other instruments, is convenient and quick, and is easy to operate.
Description
Technical Field
The invention belongs to the field of brain injury testing, relates to controlled decompression, and in particular relates to a test device for a cell controlled decompression model.
Background
The mortality rate of patients with severe craniocerebral injury can reach 30% -60%, and how to reduce the mortality rate and improve the prognosis of patients is an important topic of particular concern for neurosurgeons. Standard traumatic large bone flap decompression has been widely used in such patients.
Deboning and decompression (decompressive craniectomuy, DC) are effective methods for treating patients with refractory craniocerebral trauma. The controlled decompression in the operation can effectively reduce the incidence rate of related complications such as acute cerebral bulging in the operation, cerebral infarction after the operation and the like.
The postoperative concurrent cerebral infarction is one of serious complications after craniotomy of severe craniocerebral injury, is not rare in children, can obviously increase the mortality and disability rate of patients, and has important research significance in discussing how to reduce the incidence of cerebral infarction after craniocerebral injury operation and improve the prognosis of patients. The postoperative controlled decompression retrospective control study is adopted to treat the patients with severe craniocerebral injury of children, and the effect is good.
Based on the clinical experience, the controlled decompression method has been applied in clinic, but in the clinical application process, the controlled decompression method is operated by the experience of medical staff, no scientific system experiment is carried out on the controlled decompression of the intracranial operation by the staff, a certain regularity guide is provided for clinic, and a special device capable of carrying out the experiment on the controlled decompression is not provided in the prior art, so that the controlled decompression experiment of the system is difficult to be carried out by researchers.
Disclosure of Invention
The invention aims to provide a test device for a cell controlled decompression model, which solves the technical problem that the prior art is difficult to systematically perform controlled decompression test on cells.
In order to solve the technical problems, the invention adopts the following technical scheme:
the test device for the cell control decompression model comprises a culture dish with a sealed cover, and further comprises a flexible slide, wherein a positioning ring foot is arranged at the center of the bottom surface of the flexible slide, a positioning support seat is arranged at the center of the bottom in the culture dish, and the positioning ring foot is sleeved on the positioning support seat;
the flexible slide glass is characterized in that stress positioning holes are symmetrically formed in the periphery of the top surface of the flexible slide glass, a threaded sleeve penetrating through the cover is arranged in the center of the cover, a threaded rod is arranged in the threaded sleeve, one end of the threaded rod extending into the culture dish is connected with one end of a rotating sleeve, the rotating sleeve can rotate relative to the threaded rod, the other end of the rotating sleeve is fixedly connected to the center of a support, vertical pressurizing columns are arranged at the periphery of the support, and the pressurizing columns are propped against the pressurizing positioning holes and used for bending the flexible slide glass;
the side wall of the culture dish close to the bottom is communicated with a culture solution rapid outflow pipe;
the cover is communicated with an air inlet pipe and an air outlet pipe;
the cover is also inserted with a liquid inlet pipe and a liquid outlet pipe.
The invention also has the following distinguishing technical characteristics:
the end part of the liquid inlet pipe in the culture dish is provided with a liquid inlet drop head which spirals in a plane, and a plurality of liquid inlet holes are uniformly formed in the liquid inlet drop head; the end part of the liquid outlet pipe in the culture dish is provided with a liquid outlet suction head which spirals in a plane, and a plurality of liquid suction holes are uniformly formed in the liquid outlet suction head.
The liquid inlet drop head and the liquid outlet suction head are mutually nested in the same plane, a through hole is arranged at the center positions of the liquid inlet drop head and the liquid outlet suction head, and the threaded rod penetrates through the through hole.
The cover and the culture dish are made of transparent materials.
The transparent material is glass or transparent plastic.
The transparent plastic is organic glass.
The flexible slide is made of transparent plastic.
The thickness of the flexible slide glass is 0.2 mm-2 mm.
One end of the threaded rod extending out of the cover is provided with a rotary cap.
The number of the stressed positioning holes is four, and the bracket is a cross bracket.
Compared with the prior art, the invention has the following technical effects:
the test device can establish a set of test model of controlled decompression aiming at cells, can carry out systematic test on the controlled decompression of the cells by changing the nutrient content, the oxygen concentration and the external tension applied to the cells, is convenient for an experimenter to find the optimal controlled decompression condition, and provides beneficial guidance for clinical practice operation.
The liquid inlet drop head and the liquid outlet suction head are in a plane spiral similar to a mosquito-repellent incense shape, and the plurality of liquid inlet holes and the plurality of liquid suction holes enable liquid to be closer to cells during liquid inlet and liquid suction, and avoid external interference to the cells, which is not needed for a test.
The flexible slide glass can be taken out from the test device, is convenient to directly observe under the microscope and other instruments, is convenient and quick, and is easy to operate.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
Fig. 2 is a schematic diagram of the upper surface structures of the liquid inlet and outlet drippers.
Fig. 3 is a schematic view of the lower surface structures of the liquid inlet and outlet drippers.
Fig. 4 is a schematic structural view of the bracket.
Fig. 5 is a schematic top surface structure of a flexible slide.
Fig. 6 is a schematic view of the bottom surface structure of a flexible carrier sheet.
Fig. 7 is a schematic view of the use state of the present invention.
The meaning of each reference numeral in the figures is: the culture dish comprises a 1-cover, a 2-culture dish, a 3-flexible slide glass, a 4-positioning ring foot, a 5-positioning support seat, a 6-stressed positioning hole, a 7-threaded sleeve, an 8-threaded rod, a 9-rotating sleeve, a 10-support, an 11-pressurizing column, a 12-culture solution rapid outflow pipe, a 13-air inlet pipe, a 14-air outlet pipe, a 15-liquid inlet pipe, a 16-liquid outlet pipe, a 17-liquid inlet drop head, a 18-liquid drop hole, a 19-liquid outlet suction head, a 20-liquid suction hole, a 21-through hole, a 22-rotating cap, a 23-culture solution and 24-cells.
The following examples illustrate the invention in further detail.
Detailed Description
The following specific embodiments of the present invention are provided, and it should be noted that the present invention is not limited to the following specific embodiments, and all equivalent changes made on the basis of the technical solutions of the present application fall within the protection scope of the present invention.
Examples:
according to the technical scheme, as shown in fig. 1 to 7, the embodiment provides a test device for a cell control decompression model, which comprises a culture dish 2 with a sealed cover 1, and further comprises a flexible slide 3, wherein a positioning ring foot 4 is arranged at the central position of the bottom surface of the flexible slide 3, a positioning support seat 5 is arranged at the central position of the bottom in the culture dish 2, and the positioning ring foot 4 is sleeved on the positioning support seat 5;
the flexible slide glass 3 is characterized in that stress positioning holes 6 are symmetrically formed in the periphery of the top surface of the flexible slide glass 3, a threaded sleeve 7 penetrating through the cover is arranged in the center of the cover 1, a threaded rod 8 is arranged in the threaded sleeve 7, one end of the threaded rod 8 extending into the culture dish 2 is connected with one end of a rotating sleeve 9, the rotating sleeve 9 can rotate relative to the threaded rod 8, the other end of the rotating sleeve 9 is fixedly connected with the center of a support 10, vertical pressurizing columns 11 are arranged at the periphery of the support 10, and the pressurizing columns 11 are propped against the inside of the pressurizing positioning holes 6 and are used for bending the flexible slide glass 3;
the side wall of the culture dish 2 close to the bottom is communicated with a culture solution rapid outflow pipe 12;
the cover 1 is communicated with an air inlet pipe 13 and an air outlet pipe 14;
the cover 1 is also inserted with a liquid inlet pipe 15 and a liquid outlet pipe 16.
The end part of the liquid inlet pipe 15 in the culture dish 2 is provided with a liquid inlet drop head 17 which spirals in a plane, and a plurality of liquid inlet holes 18 are uniformly formed in the liquid inlet drop head 17; the end of the liquid outlet pipe 16 in the culture dish 2 is provided with a liquid outlet suction head 19 which spirals in a plane, and a plurality of liquid suction holes 20 are uniformly arranged on the liquid outlet suction head 19.
The droplet inlet head 17 and the liquid outlet head 19 are nested in the same plane, which facilitates better access to the flexible slide 3 and does not affect the cells 23. The center of the liquid inlet head 17 and the liquid outlet head 19 is provided with a through hole 21, and the threaded rod 8 passes through the through hole 21.
The cover 1 and the culture dish 2 are made of transparent materials, the transparent materials are glass or transparent plastics, and the transparent plastics are preferably organic glass, so that the observation is convenient.
The flexible slide 3 is made of transparent plastic, meets the requirements of flexible bending and recovery, and can be directly observed under a microscope. The thickness of the flexible slide glass 3 is 0.2 mm-2 mm, and the flexible slide glass 3 with certain column thickness can be designed according to test requirements and used for simulating different external expansion forces, so that a plurality of column controlled decompression tests can be conveniently carried out.
The threaded rod 8 is provided with a turning cap 22 at the end protruding from the cover 1 for selecting the threaded rod 8 and thus squeezing the flexible slide 3.
The four stress positioning holes 6 are arranged, the supports 10 are cross supports, the four sides of the flexible slide 3 are uniformly pressed in a one-to-one correspondence manner, the positioning holes 6 can be replaced by annular positioning grooves, and the pressurizing columns 11 can be replaced by corresponding annular protrusions, so that the stress is uniform.
The culture dish 2 is used for containing culture solution 23, and the flexible slide 3 is used for attaching cells 24.
The shape of the flexible carrier sheet 3 is selected according to the need, preferably circular.
The working process of the invention is as follows:
when the test device is used, the flexible slide glass 3 is placed in the culture dish 2, and positioning and installation are realized through the matching of the positioning ring feet 4 and the positioning support seats 5. The normal culture solution is poured into the culture dish 2, then cells are cultured in the culture solution, the cells are attached to the flexible slide 3, the cover 1 is covered, the pressurizing column 11 is matched with the stress positioning hole 6 but does not generate pressure, and the culture dish 2 is sealed. According to the experimental design, during the test, the normal culture solution is discharged through the culture solution quick outflow pipe 12, various nutrient-insufficient culture solutions are provided for cells through the liquid inlet pipe 15 and the liquid inlet drop head 17, and the plurality of liquid dropping holes 18 drop the liquid, so that the extra interference to the cells is reduced as much as possible. The cells are supplied with gases of different oxygen contents through the gas inlet pipe 13 and the gas outlet pipe 14.
The threaded rod 8 is rotated by the rotating cap 22, so that the threaded rod 8 vertically feeds in the threaded sleeve 7, the rotation of the threaded rod 8 gives pressure to the rotating sleeve 9, the pressure is transmitted to the pressurizing column 11 through the bracket 10, the peripheral part of the flexible slide 3 is pressurized, symmetrical deformation is generated on the flexible slide 3, different external tensions of cells are given by controlling the feeding amount of the threaded rod 8, then the pressure is gradually reduced according to test requirements, and the controlled depressurization is simulated.
After the test is performed to a certain extent, the culture solution with low nutrient content needs to be gradually replaced by the culture solution with normal nutrient content through the cooperation of the liquid inlet nozzle 17 and the liquid outlet nozzle 19. By simulating the above-described various conditions, a systematic test of controlled decompression of cells can be performed. After the test is completed, the flexible slide 3 is taken out together with the cells 24 attached thereto, and can be observed under a microscope.
Claims (7)
1. A test device for a cell-controlled decompression model, comprising a culture dish (2) with a sealed lid (1), characterized in that: the culture dish is characterized by further comprising a flexible slide glass (3), wherein a positioning ring foot (4) is arranged at the center of the bottom surface of the flexible slide glass (3), a positioning support seat (5) is arranged at the center of the bottom in the culture dish (2), and the positioning ring foot (4) is sleeved on the positioning support seat (5);
the flexible slide glass is characterized in that stress positioning holes (6) are symmetrically formed in the periphery of the top surface of the flexible slide glass (3), a threaded sleeve (7) penetrating through the cover is arranged in the center of the cover (1), a threaded rod (8) is arranged in the threaded sleeve (7), one end of the threaded rod (8) extending into the culture dish (2) is connected with one end of a rotating sleeve (9), the rotating sleeve (9) can rotate relative to the threaded rod (8), the other end of the rotating sleeve (9) is fixedly connected to the center of a support (10), vertical compression columns (11) are arranged at the periphery of the support (10), and the compression columns (11) are propped against the inside of the compression positioning holes (6) and are used for bending the flexible slide glass (3);
the side wall of the culture dish (2) close to the bottom is communicated with a culture solution rapid outflow pipe (12);
an air inlet pipe (13) and an air outlet pipe (14) are communicated with the cover (1);
a liquid inlet pipe (15) and a liquid outlet pipe (16) are also inserted into the cover (1);
the end part of the liquid inlet pipe (15) in the culture dish (2) is provided with a liquid inlet drop head (17) which spirals in a plane, and a plurality of liquid inlet holes (18) are uniformly formed in the liquid inlet drop head (17); the end part of the liquid outlet pipe (16) in the culture dish (2) is provided with a liquid outlet suction head (19) which spirals in a plane, and a plurality of liquid suction holes (20) are uniformly formed in the liquid outlet suction head (19);
the thickness of the flexible slide glass (3) is 0.2 mm-2 mm;
one end of the threaded rod (8) extending out of the cover (1) is provided with a rotary cap (22).
2. The test device of claim 1, wherein: the liquid inlet drop head (17) and the liquid outlet suction head (19) are mutually nested in the same plane, a through hole (21) is arranged at the center of the liquid inlet drop head (17) and the center of the liquid outlet suction head (19), and the threaded rod (8) passes through the through hole (21).
3. The test device of claim 1, wherein: the cover (1) and the culture dish (2) are made of transparent materials.
4. A test device as claimed in claim 3, wherein: the transparent material is glass or transparent plastic.
5. The test device of claim 4, wherein: the transparent plastic is organic glass.
6. The test device of claim 1, wherein: the flexible slide glass (3) is made of transparent plastic.
7. The test device of claim 1, wherein: the number of the stressed positioning holes (6) is four, and the bracket (10) is a cross bracket.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610809076.8A CN106353490B (en) | 2016-09-07 | 2016-09-07 | Test device for cell control decompression model |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610809076.8A CN106353490B (en) | 2016-09-07 | 2016-09-07 | Test device for cell control decompression model |
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| Publication Number | Publication Date |
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| CN106353490A CN106353490A (en) | 2017-01-25 |
| CN106353490B true CN106353490B (en) | 2023-05-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610809076.8A Active CN106353490B (en) | 2016-09-07 | 2016-09-07 | Test device for cell control decompression model |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1168921A (en) * | 1996-06-18 | 1997-12-31 | 廖明一 | Method and apparatus for culturing attached cells |
| CN2818484Y (en) * | 2005-07-15 | 2006-09-20 | 中国人民解放军第四军医大学 | Cell tractive tension controller |
| JP2008092935A (en) * | 2006-10-13 | 2008-04-24 | Otake:Kk | Culturing apparatus in which pump function is provided in hermetically sealed culture container |
| CN201737949U (en) * | 2010-07-12 | 2011-02-09 | 中国人民解放军第三军医大学第一附属医院 | Cell culturing device capable of periodically converting positive pressure and negative pressure |
| CN201815216U (en) * | 2010-09-15 | 2011-05-04 | 李锦记（新会）食品有限公司 | Vacuum brewing material suction solid-gas separator |
-
2016
- 2016-09-07 CN CN201610809076.8A patent/CN106353490B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1168921A (en) * | 1996-06-18 | 1997-12-31 | 廖明一 | Method and apparatus for culturing attached cells |
| CN2818484Y (en) * | 2005-07-15 | 2006-09-20 | 中国人民解放军第四军医大学 | Cell tractive tension controller |
| JP2008092935A (en) * | 2006-10-13 | 2008-04-24 | Otake:Kk | Culturing apparatus in which pump function is provided in hermetically sealed culture container |
| CN201737949U (en) * | 2010-07-12 | 2011-02-09 | 中国人民解放军第三军医大学第一附属医院 | Cell culturing device capable of periodically converting positive pressure and negative pressure |
| CN201815216U (en) * | 2010-09-15 | 2011-05-04 | 李锦记（新会）食品有限公司 | Vacuum brewing material suction solid-gas separator |
Non-Patent Citations (1)
| Title |
|---|
| 机械压力及细胞内钙释放通道阻滞对髁突软骨细胞骨架影响的研究;张昊,王美青,王景杰;口腔医学研究(第03期);全文 * |
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| CN106353490A (en) | 2017-01-25 |
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