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CN205420413U - Many functional type laboratory photobioreactor system - Google Patents

Many functional type laboratory photobioreactor system Download PDF

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Publication number
CN205420413U
CN205420413U CN201520952702.XU CN201520952702U CN205420413U CN 205420413 U CN205420413 U CN 205420413U CN 201520952702 U CN201520952702 U CN 201520952702U CN 205420413 U CN205420413 U CN 205420413U
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reactor
steam line
laboratory
gas supply
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余江
冉宗信
王萍
苏子艺
王月圆
孔璇
张宁
刘权震
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Sichuan University
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Sichuan University
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Abstract

The utility model relates to a many functional type laboratory photobioreactor system. Including air feed part, reation kettle, auxiliary device part, auxiliary device includes the mounting bracket of reation kettle bottom and the illumination device on the mounting bracket, the air feed part comprises gas storage steel cylinder, air pump, air feed control system, gas supply line, and gas storage steel cylinder passes through the steel bottle branch pipe and is connected with the gas supply line, the air pump passes through air branch pipe and is connected with the gas supply line, the gas supply line inserts among the reation kettle, air feed control system is including setting up motorised valve and the flowmeter on steel bottle branch pipe, air branch pipe, gas supply line, last thermometer, gaseous detector, the air -blowing balloon of being provided with of reation kettle. The utility model discloses equipment is simply easily operated, and detachability is high, easily transport, and the ration regulation and control to the various parameters of ventilating of environment index 's such as temperature, pH regulation and control and laboratory indispensability can be realized to the while. Can extensively be applicable to cultivation and experimental study that ordinary laboratory is used for little algae. (B /)

Description

一种多功能型实验室光生物反应器系统A multifunctional laboratory photobioreactor system

技术领域 technical field

本实用新型涉及用于实验室微藻培养的多功能型实验室光生物反应器装置。 The utility model relates to a multifunctional laboratory photobioreactor device for laboratory microalgae cultivation.

背景技术 Background technique

实验室光生物反应器主要应用于实验室微藻培养,在给定条件下对微藻生长特性或者培养条件的研究,在特定情况下对培养基进行生化处理的研究,以及通过培养微藻产能,生产新型生物能源的实验研究等。 Laboratory photobioreactors are mainly used in the cultivation of microalgae in the laboratory, the research on the growth characteristics or culture conditions of microalgae under given conditions, the research on the biochemical treatment of the medium under certain circumstances, and the production of microalgae by cultivating microalgae. , Experimental research on the production of new bioenergy, etc.

在现有的实验室光生物反应器中,大多都具有设备精密度高,生物培养体系安全高效,性能稳定,安全稳固性好等优点。然而另一方面,这些光生物反应器大多为培养体系和条件控制一体化控制装置,这些装置多采用具有高技术含量的电子控调设备生产组装而成,因此,这一类光生物反应装置成品其价格昂贵,对于一般小型实验室来说因购买能力有限而难以配备。并且这样一体式的实验室光生物反应器还具有体积较大,可拆卸性低,不易搬运等特点,在使用中不是很便捷。 Most of the existing laboratory photobioreactors have the advantages of high equipment precision, safe and efficient biological culture system, stable performance, and good safety and stability. However, on the other hand, most of these photobioreactors are integrated control devices for culture system and condition control, and these devices are mostly produced and assembled by electronic control and adjustment equipment with high technical content. It is expensive, and it is difficult to equip small laboratories due to limited purchasing power. Moreover, such an integrated laboratory photobioreactor also has the characteristics of large volume, low detachability, and difficult handling, which is not very convenient in use.

同时,实验室微藻培养过程中,不仅需要给予试验温度、反应釜内的液体PH值、光照强度与时间等条件的设立与控制,同时还应当对通气浓度、体积、速率和方式等条件进行精确调控。但现有的光生物反应器只限于温度、PH值、光照等环境因素的调节和控制,少有针对例如通气浓度、体积、速率和方式等条件进行精确调控,因此造成试验条件不完备。 At the same time, during the cultivation of microalgae in the laboratory, it is necessary not only to set up and control conditions such as test temperature, liquid pH value in the reactor, light intensity and time, but also to control conditions such as aeration concentration, volume, rate and method. Precise regulation. However, the existing photobioreactors are only limited to the adjustment and control of environmental factors such as temperature, pH value, and light, and seldom carry out precise adjustments on conditions such as ventilation concentration, volume, rate, and method, thus resulting in incomplete test conditions.

实用新型内容 Utility model content

为了解决上述问题,本实用新型提供了一种多功能型实验室光生物反应器。 In order to solve the above problems, the utility model provides a multifunctional laboratory photobioreactor.

本实用新型的实验室光生物反应器包括供气部分、反应釜、辅助装置部分。所述的供气部分通过供气管道4连接到反应釜9。辅助装置包括反应釜底部的安装架14以及安装架上的光照装置15,光照装置用于为反应釜提供光照。供气部分由储气钢瓶12、空气泵13、供气控制系统、供气管道4构成,储气钢瓶通过钢瓶支管1与供气管道连接。空气泵13通过空气支管2与供气管道连接,用于提供自然空气。供气管道4插入到反应釜中。供气控制系统包括设置在钢瓶支管、空气支管、供气管道上的电动阀8与流量计7。所述的反应釜上设置有温度计16、气体检测仪10、鼓气气球6。 The laboratory photobioreactor of the utility model includes an air supply part, a reaction kettle and an auxiliary device part. The gas supply part is connected to the reactor 9 through the gas supply pipeline 4 . The auxiliary device includes a mounting frame 14 at the bottom of the reactor and a lighting device 15 on the mounting frame, and the lighting device is used to provide light for the reactor. The air supply part is composed of a gas storage cylinder 12, an air pump 13, an air supply control system, and an air supply pipeline 4, and the gas storage cylinder is connected with the gas supply pipeline through a steel cylinder branch pipe 1. The air pump 13 is connected with the air supply pipeline through the air branch pipe 2, and is used to provide natural air. The gas supply pipe 4 is inserted into the reactor. The gas supply control system includes an electric valve 8 and a flow meter 7 arranged on the steel cylinder branch pipe, the air branch pipe and the gas supply pipeline. The reaction kettle is provided with a thermometer 16, a gas detector 10, and an inflating balloon 6.

如上所述的多功能型实验室光生物反应器,具体为,所述的供气控制系统还包括有自动控制器11,钢瓶支管1与空气支管2上均设置有电动阀8与流量计7。自动控制器分别连接电动阀、流量计,用于通过测得钢瓶支管与空气支管中的气体流量并控制电动阀。供气管道4上设置有气压表5,用于测量反应釜内的气压。 The above-mentioned multifunctional laboratory photobioreactor, specifically, the air supply control system also includes an automatic controller 11, and electric valves 8 and flowmeters 7 are provided on the steel cylinder branch pipe 1 and the air branch pipe 2 . The automatic controller is respectively connected with the electric valve and the flow meter, and is used to measure the gas flow in the steel cylinder branch pipe and the air branch pipe and control the electric valve. The air supply pipeline 4 is provided with a barometer 5 for measuring the air pressure in the reactor.

如上所述的多功能型实验室光生物反应器,具体为,所述的反应釜为封闭式玻瓶反应釜,反应釜顶部为软胶瓶塞3,供气管道4、温度计16、气体检测仪10、鼓气气球6均从软胶瓶塞插入到反应釜中。 The multifunctional laboratory photobioreactor as described above, specifically, the reactor is a closed glass bottle reactor, the top of the reactor is a soft rubber stopper 3, the gas supply pipeline 4, a thermometer 16, a gas detection Instrument 10 and inflating balloon 6 are all inserted into the reactor from the soft rubber stopper.

如上所述的多功能型实验室光生物反应器,具体为,所述的安装架14包括底座与底座上的钢化玻璃板,反应釜设置在钢化玻璃上方,在钢化玻璃板下方安装光照装置15。所述的光照装置为灯。 The multifunctional laboratory photobioreactor as described above, specifically, the mounting frame 14 includes a base and a tempered glass plate on the base, the reaction kettle is arranged above the tempered glass, and an illumination device 15 is installed under the tempered glass plate . The lighting device is a lamp.

本实用新型的有益效果如下: The beneficial effects of the utility model are as follows:

本实用新型结构简单,易于拆卸与搬运,同时在生物培养方面实现了除一般实验室光生物反应器具有pH、光照、温度等环境条件外,还能够实现对对生物培养过程中通气二氧化碳浓度、体积、速率、时间间隔的调控,可测定pH、温度、通气二氧化碳浓度、气压、光照强度等指标,实现对样品的随时采样和加料的便利性,可模拟自然环境,同时避免了传统密闭式光生物反应器反应体积过大,占地面积大和生产成本高的缺点,结合了传统开放式光生物反应器生产成本低,占地面积较小的优点,可广泛适用于普通小型实验室用于微藻的培养和实验研究,起到了简单易安装,操作性强的效果,适合现今实验室装置的市场需求。 The utility model has a simple structure and is easy to disassemble and carry. At the same time, in addition to the environmental conditions such as pH, light, and temperature of the general laboratory photobioreactor, it can also realize the adjustment of the concentration of carbon dioxide and the concentration of aerated carbon dioxide during the biological cultivation process. The regulation of volume, rate and time interval can measure pH, temperature, ventilation carbon dioxide concentration, air pressure, light intensity and other indicators, realize the convenience of sampling and feeding samples at any time, simulate the natural environment, and avoid the traditional closed light The bioreactor has the shortcomings of large reaction volume, large floor area and high production cost, combined with the advantages of low production cost and small floor space of the traditional open photobioreactor, it can be widely used in ordinary small laboratories for micro The cultivation and experimental research of algae has the effect of simple installation and strong operability, which is suitable for the market demand of today's laboratory equipment.

附图说明 Description of drawings

图1是本实用新型的结构图。 Fig. 1 is a structural diagram of the utility model.

具体实施方式 detailed description

下面结合附图和实例对本实用新型进一步说明。 Below in conjunction with accompanying drawing and example the utility model is further described.

本实用新型的实验室光生物反应器包括供气部分、反应釜、辅助装置部分。所述的供气部分通过供气管道4连接到反应釜9。 The laboratory photobioreactor of the utility model includes an air supply part, a reaction kettle and an auxiliary device part. The gas supply part is connected to the reactor 9 through the gas supply pipeline 4 .

供气部分由储气钢瓶12、空气泵13、供气控制系统、供气管道4构成,储气钢瓶通过钢瓶支管1与供气管道连接。空气泵13通过空气支管2与供气管道连接,用于提供自然空气。供气管道4插入到反应釜中。供气控制系统包括设置在钢瓶支管、空气支管、供气管道上的电动阀8与流量计7。 The air supply part is composed of a gas storage cylinder 12, an air pump 13, an air supply control system, and an air supply pipeline 4, and the gas storage cylinder is connected with the gas supply pipeline through a steel cylinder branch pipe 1. The air pump 13 is connected with the air supply pipeline through the air branch pipe 2, and is used to provide natural air. The gas supply pipe 4 is inserted into the reactor. The gas supply control system includes an electric valve 8 and a flow meter 7 arranged on the steel cylinder branch pipe, the air branch pipe and the gas supply pipeline.

供气控制系统还包括有自动控制器11,钢瓶支管1与空气支管2上均设置有电动阀8与流量计7。自动控制器分别连接电动阀、流量计,用于通过测得钢瓶支管与空气支管中的气体流量并控制电动阀。供气管道4上设置有气压表5,用于测量反应釜内的气压。此处的供气控制系统可以为微电脑芯片或具备自动控制能力的其它电子装置,对电动阀的控制主要是开启时间的控制与开启流量大小的控制,供气控制系统至少安装有时间继电器,在每隔一个设定的时间后,自动开启电动阀,对反应釜内进行间歇式供气。 The gas supply control system also includes an automatic controller 11 , and electric valves 8 and flowmeters 7 are provided on the steel cylinder branch pipe 1 and the air branch pipe 2 . The automatic controller is respectively connected with the electric valve and the flow meter, and is used to measure the gas flow in the steel cylinder branch pipe and the air branch pipe and control the electric valve. The air supply pipeline 4 is provided with a barometer 5 for measuring the air pressure in the reactor. The air supply control system here can be a microcomputer chip or other electronic devices with automatic control capabilities. The control of the electric valve is mainly the control of the opening time and the control of the opening flow. The air supply control system is at least equipped with a time relay. After every set time, the electric valve is automatically opened to supply air intermittently to the reactor.

储气钢瓶的出口设置减压阀。在实验过程中,减压阀处于开启状态。 The outlet of the gas storage cylinder is provided with a pressure reducing valve. During the experiment, the pressure reducing valve was kept open.

辅助装置包括反应釜底部的安装架14以及安装架上的光照装置15,光照装置用于为反应釜提供光照。所述的安装架14包括底座与底座上的钢化玻璃板,反应釜设置在钢化玻璃上方,在钢化玻璃板下方安装光照装置15。采用钢化玻璃,使安装架具备承载能力的同时,又有极高的透光度。底座采用铝合金钢架,材质更轻,易于搬运。所述的光照装置为日光灯或LED灯,并采用旋钮开关控制,调节光的照度,具体光度可采用照度计定量测定。 The auxiliary device includes a mounting frame 14 at the bottom of the reactor and a lighting device 15 on the mounting frame, and the lighting device is used to provide light for the reactor. The mounting frame 14 includes a base and a tempered glass plate on the base, the reaction kettle is arranged above the tempered glass, and an illumination device 15 is installed under the tempered glass plate. The use of toughened glass enables the mounting frame to have a load-bearing capacity and at the same time have extremely high light transmittance. The base is made of aluminum alloy steel frame, which is lighter in material and easy to carry. The illumination device is a fluorescent lamp or an LED lamp, and is controlled by a rotary switch to adjust the illuminance of the light. The specific illuminance can be quantitatively measured by an illuminance meter.

所述的反应釜上设置有温度计16、气体检测仪10、鼓气气球6。反应釜为封闭式玻瓶反应釜,反应釜顶部为软胶瓶塞3,软胶瓶塞上设有四个孔洞,用于插入供气管道4、温度计16、气体检测仪10、鼓气气球6,并保证气密性。气体检测仪主要用于检测反应釜内液体的pH值,当然也可以根据需要检测其它参数。软胶瓶塞3 The reaction kettle is provided with a thermometer 16, a gas detector 10, and an inflating balloon 6. The reaction kettle is a closed glass bottle reaction kettle, the top of the reaction kettle is a soft rubber stopper 3, and there are four holes on the soft rubber stopper for inserting the gas supply pipeline 4, thermometer 16, gas detector 10, and inflating balloon 6, and ensure airtightness. The gas detector is mainly used to detect the pH value of the liquid in the reactor, and of course other parameters can be detected as needed. Soft rubber stopper 3

另外瓶口安置一个固定钢圈实现对气球的固定。连接气球的通气管设置为可伸缩管,气球设置为可拆卸式,既能满足持续性通气实验的要求也能满足间歇式通气测定气体去除率的实验要求。反应釜最下端设置一个出样口,出样口为玻璃旋钮式可实现对样品的微量取样。气体检测仪(PH计)、温度计分别对反应釜内液体的pH和温度进行检测,从而获得微生物培养过程中的基本参数。 In addition, a fixed steel ring is placed on the bottle mouth to realize the fixing of the balloon. The ventilation tube connected to the balloon is set as a telescopic tube, and the balloon is set as a detachable type, which can meet the requirements of the continuous ventilation experiment and the experimental requirements of the intermittent ventilation to measure the gas removal rate. A sample outlet is set at the bottom of the reaction kettle, and the sample outlet is a glass knob type, which can realize micro-sampling of the sample. The gas detector (PH meter) and thermometer detect the pH and temperature of the liquid in the reactor respectively, so as to obtain the basic parameters in the process of microbial cultivation.

上面已结合附图对本实用新型的具体实施方式进行实例性的描述,显然本实用新型不限于此,在本实用新型范围内进行的各种改型均没有超出本实用新型的应用范围。 The specific implementation of the utility model has been described above in conjunction with the accompanying drawings. Obviously, the utility model is not limited thereto, and various modifications within the scope of the utility model are within the scope of application of the utility model.

Claims (4)

1. a Mobyneb laboratory photo-bioreactor system, including supply part, reactor, auxiliary device part;Described supply part is connected to reactor (9) by steam line (4);Auxiliary device includes the installing rack (14) bottom reactor and the illumination apparatus (15) on installing rack, and illumination apparatus is for providing illumination for reactor;Supply part is made up of gas storage cylinder (12), air pump (13), supply control system, steam line (4), and gas storage cylinder is connected with steam line by steel cylinder arm (1);Air pump (13) is connected with steam line by air arm (2), is used for providing natural air;Steam line (4) is inserted in reactor;Supply control system includes electrodynamic valve (8) and the effusion meter (7) being arranged on steel cylinder arm, air arm, steam line;Thermometer (16), gas detector (10), air-blowing balloon (6) it is provided with on described reactor.
2. a kind of Mobyneb laboratory photo-bioreactor system as claimed in claim 1, it is characterized in that, described supply control system also includes automatic controller (11), and steel cylinder arm (1) and air arm (2) are provided with electrodynamic valve (8) and effusion meter (7);Automatic controller connects electrodynamic valve, effusion meter respectively, is used for the gas flow by recording in steel cylinder arm and air arm and controls electrodynamic valve;Air gauge (5) it is provided with, for measuring the air pressure in reactor on steam line (4).
3. a kind of Mobyneb laboratory photo-bioreactor system as claimed in claim 1, it is characterized in that, described reactor is closed glass bottle reactor, reactor top is flexible glue bottle stopper (3), and steam line (4), thermometer (16), gas detector (10), air-blowing balloon (6) are all inserted into reactor from flexible glue bottle stopper.
4. a kind of Mobyneb laboratory photo-bioreactor system as claimed in claim 1, it is characterized in that, described installing rack (14) includes base and the armorplate glass on base, reactor is arranged on above safety glass, installs illumination apparatus (15) below armorplate glass;Described illumination apparatus is lamp.
CN201520952702.XU 2015-11-26 2015-11-26 Many functional type laboratory photobioreactor system Expired - Fee Related CN205420413U (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107164217A (en) * 2017-07-07 2017-09-15 苏州富园生物科技有限公司 A kind of efficient microalgae apparatus for photoreaction
CN107904144A (en) * 2017-12-06 2018-04-13 山西农业大学 Microalgae column reactor is aerated disturbing flow device
CN108046491A (en) * 2017-12-13 2018-05-18 中南民族大学 Portable accurately controls the device of light degradation organic matter

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107164217A (en) * 2017-07-07 2017-09-15 苏州富园生物科技有限公司 A kind of efficient microalgae apparatus for photoreaction
CN107904144A (en) * 2017-12-06 2018-04-13 山西农业大学 Microalgae column reactor is aerated disturbing flow device
CN108046491A (en) * 2017-12-13 2018-05-18 中南民族大学 Portable accurately controls the device of light degradation organic matter

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