CN106353490A - Testing unit for cell control type pressure relief model - Google Patents
Testing unit for cell control type pressure relief model Download PDFInfo
- Publication number
- CN106353490A CN106353490A CN201610809076.8A CN201610809076A CN106353490A CN 106353490 A CN106353490 A CN 106353490A CN 201610809076 A CN201610809076 A CN 201610809076A CN 106353490 A CN106353490 A CN 106353490A
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- culture dish
- assay device
- flexible slide
- threaded rod
- slide glass
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The invention provides a testing unit for cell control type pressure relief model. The testing unit is characterized in that a positioning ring foot is arranged at the position of the bottom center of a flexibly slide, a positioning supporting seat is arranged at the position of the bottom center in a culture dish, and the positioning ring foot sleeves the positioning supporting seat; stress positioning holes are symmetrically arranged in positions around the top surface of the flexible slide, a threaded sleeve communicating with a cover is arranged at the position of the center on the cover, a threaded rod is mounted in the threaded sleeve and extends one half in the culture dish to be connected to one end of a rotating sleeve, the rotating sleeve is capable of rotating relative to the threaded rod, the other end of the rotating sleeve is fixedly connected to the position of the center of a support, pressured columns are vertically arranged at end portions of the periphery of the support, and the pressured columns are abutted against pressured holes to be used for bending the flexible slide. By the arrangement, the flexible slide can be taken out from the testing unit, direct observation under instruments of a microscope and the like is facilitated, and the testing unit is convenient and rapid and easy to operate.
Description
Technical field
The invention belongs to brain injury field tests, it is related to controlling decompression and in particular to one kind subtracts for cell controlling
The assay device of pressing mold type.
Background technology
How levels of thyroid hormone in craniocerebral trauma patients mortality rate, up to 30%～60%, reduces mortality rate and improves the prognosis of patient and be
The important topic that neurosurgical doctor pays special attention to.Standard large trauma craniotomy has obtained extensively should in such patient
With.
Decompressive craniectomy (decompressive craniectomuy, dc) is the treatment intractable cranium of severe traumatic head injury
The effective ways of high pressure patient.In art, controlling decompression is effectively reduced the correlations such as acute encephalocele in art, postoperative cerebral infarction simultaneously
Send out the incidence rate of disease.
Complicated by postoperative cerebral infarction be craniotomy on patients with severe craniocerebral injury after one of severe complication, see child is quite a few,
Mortality rate and the disability rate of patient can substantially be increased, inquire into how to reduce severe cranial brain injury cerebral infarction incidence rate and improvement
The prognosis of patient has important research meaning.Using controlling decompression retrospective comparative study operative treatment child heavy type cranium in art
Brain injury patient, respond well.
Based on above-mentioned clinical experience, controlling decompression method has clinically had more application, but in clinical practice
In journey, being operated, still nobody carries out science to the controlling decompression of encephalic operation to the more experience relying on medical personnel
System experimentation, provides certain law to guide for clinical, does not also have to be tested for controlling decompression in prior art
Special purpose device so that research worker is difficult to the controlling decompression test of system.
Content of the invention
In view of the shortcomings of the prior art, it is an object of the invention to, provide and a kind of reduce pressure mould for cell controlling
The assay device of type, solves the technical problem being difficult in prior art systematically be directed to cell being controlled property decompression test.
In order to solve above-mentioned technical problem, the present invention adopts the following technical scheme that and is achieved:
A kind of assay device for cell controlling pressure relief model, including the culture dish with Packed lid, also wraps
Include flexible slide glass, the bottom center position of described flexible slide glass is provided with positioning ring foot, in the bottom in described culture dish
Heart position is provided with positioning support base, and centring ring foot cover is on positioning support base；
The top surface surrounding positional symmetry of described flexible slide glass is provided with stress location hole, the centre bit on described lid
Install the screw shell being equipped with insertion lid, threaded rod is installed in screw shell, threaded rod stretches into the one end within culture dish
It is connected with one end of rotational sleeve, rotational sleeve can rotate with respect to threaded rod, and the other end of rotational sleeve is fixed in support
Center, the end of support surrounding is provided with vertical pressured column, pressured column withstand on pressurization positioning in the hole be used for bending soft
Property slide glass；
Described culture dish near bottom side wall on be communicated with the quick effuser of culture fluid；
Air inlet pipe and escape pipe are communicated with described lid；
Feed tube and drain pipe are also inserted with described lid.
The present invention also has a following distinguishing feature:
End in culture dish for the described feed tube is provided with the feed liquor water dropper planar spiraling, on feed liquor water dropper all
Even it is provided with multiple dripping holes；End in culture dish for the described drain pipe be provided with planar spiral go out liquid suction nozzle,
Go out and multiple imbibing holes are evenly arranged with liquid suction nozzle.
Described feed liquor water dropper mutually nested with going out liquid suction nozzle in approximately the same plane, described feed liquor water dropper and go out liquid and inhale
The center of head arranges a through hole, and threaded rod passes through from through hole.
Described lid and culture dish are all made using transparent material.
Described transparent material is glass or transparent plastic.
Described transparent plastic is lucite.
Described flexible slide glass is made using transparent plastics.
The thickness of described flexible slide glass is 0.2mm～2mm.
Described threaded rod stretches out and is provided with rotating cap on one end of lid.
Described stress location hole is four, and described support is cross frame.
The present invention compared with prior art, has the following technical effect that
The assay device of the present invention, can set up the test model of a set of controlling decompression, be trained by changing for cell
The nutrient composition content of nutrient solution, oxygen concentration and to cell apply external tension, the controlling of cell can be reduced pressure into
The test of row system, is easy to the controlling reduced pressure that experimenter finds optimum, provides beneficial finger for clinical practice operation
Lead.
The feed liquor water dropper of the present invention spirals with going out the plane that liquid suction nozzle is similar mosquito incense shape, multiple dripping holes and imbibing hole,
Enable when dropping liquid and imbibition closer to cell, it is to avoid cell is caused with the unwanted external interference of test institute.
The flexible slide glass of the present invention can take out from assay device, is easy to directly observe under the instruments such as microscope, side
Just fast it is easy to operate.
Brief description
Fig. 1 is the overall structure diagram of the present invention.
Fig. 2 is feed liquor water dropper and the surface structure schematic diagram going out drop head.
Fig. 3 is feed liquor water dropper and the lower surface configuration schematic diagram going out drop head.
Fig. 4 is the structural representation of support.
Fig. 5 is the top surface structure schematic diagram of flexible slide glass.
Fig. 6 is the bottom surface structure schematic diagram of flexible slide glass.
Fig. 7 is the use state schematic diagram of the present invention.
The implication of each label of in figure is: 1- lid, 2- culture dish, 3- flexibility slide glass, and 4- positions ring foot, and 5- positioning supports
Seat, 6- stress location hole, 7- screw shell, 8- threaded rod, 9- rotational sleeve, 10- support, 11- pressured column, 12- culture fluid is fast
Fast effuser, 13- air inlet pipe, 14- escape pipe, 15- feed tube, 16- drain pipe, 17- feed liquor water dropper, 18- dripping hole, 19- goes out
Liquid suction nozzle, 20- imbibing hole, 21- through hole, 22- rotating cap, 23- culture fluid, 24- cell.
With reference to embodiments explanation is further explained in detail to the particular content of the present invention.
Specific embodiment
The specific embodiment of the present invention given below, implements in detail below it should be noted that the invention is not limited in
Example, all equivalents done on the basis of technical scheme each fall within protection scope of the present invention.
Embodiment:
Defer to technique scheme, as shown in Figures 1 to 7, the present embodiment provides one kind for cell controlling decompression mould
The assay device of type, including the culture dish 2 with Packed lid 1, also includes flexible slide glass 3, the bottom of described flexible slide glass 3
Face center is provided with positioning ring foot 4, and the bottom centre position in described culture dish 2 is provided with positioning support base 5, positioning
Ring foot 4 is enclosed within positioning support base 5；
The top surface surrounding positional symmetry of described flexible slide glass 3 is provided with stress location hole 6, on described lid 1 in
Heart position is provided with the screw shell 7 of insertion lid, is provided with threaded rod 8 in screw shell 7, and threaded rod 8 stretches in culture dish 2
The one end in portion is connected with one end of rotational sleeve 9, and rotational sleeve 9 can rotate with respect to threaded rod 8, rotational sleeve 9 another
End is fixed in the center of support 10, and the end of support 10 surrounding is provided with vertical pressured column 11, and pressured column 11 withstands on and adds
It is used for, in pressure location hole 6, the flexible slide glass 3 that bends；
Described culture dish 2 near bottom side wall on be communicated with the quick effuser of culture fluid 12；
Air inlet pipe 13 and escape pipe 14 are communicated with described lid 1；
Feed tube 15 and drain pipe 16 are also inserted with described lid 1.
End in culture dish 2 for the described feed tube 15 is provided with the feed liquor water dropper 17 planar spiraling, and enters drop
It is evenly arranged with multiple dripping holes 18 on 17；End in culture dish 2 for the described drain pipe 16 is provided with planar disk
Revolve goes out liquid suction nozzle 19, goes out and is evenly arranged with multiple imbibing holes 20 on liquid suction nozzle 19.
Feed liquor water dropper 17 is mutually nested with going out liquid suction nozzle 19 to be easy to have better access to flexible slide glass 3 in approximately the same plane,
And do not affect cell 23.Described feed liquor water dropper 17 and one through hole 21 of center setting going out liquid suction nozzle 19, threaded rod 8
Pass through from through hole 21.
Lid 1 and culture dish 2 are all made using transparent material, are easy to observe, and transparent material is glass or transparent moulds
Material, transparent plastic is preferably lucite.
Flexible slide glass 3 is made using transparent plastics, meets flexible bending and the demand recovered, and can be in microscope
Lower directly observation.The thickness of flexible slide glass 3 is 0.2mm～2mm, can be according to the flexible load of test some row thickness of Demand Design
Piece 3, for simulating different extraneous expansionary forces, is convenient for some row controlling decompression tests.
Threaded rod 8 stretches out and is provided with rotating cap 22 on one end of lid 1, for selecting threaded rod 8, and then extrudes flexible load
Piece 3.
Stress location hole 6 is four, and described support 10 is cross frame, corresponds, and applies to flexible slide glass 3 surrounding
Uniform pressure, location hole 6 can also be replaced with annular positioning groove, and pressured column 11 be could alternatively be corresponding annular protrusion,
Make pressurized uniform.
Culture dish 2 is used for holding culture fluid 23, and flexible slide glass 3 is used for attached cell 24.
The shape of flexible slide glass 3 to select as needed, circular.
The work process of the present invention is as described below:
When the assay device of the present invention uses, flexible slide glass 3 is placed in culture dish 2, by positioning ring foot 4 and positioning
Location and installation is realized in the cooperation of support base 5.Normal culture fluid is poured in culture dish 2, then cultured cells in culture fluid,
Cell attachment, on flexible slide glass 3, closes the lid 1 so that pressured column 11 and stress location hole 6 coordinate but do not produce pressure, makes
Obtain culture dish 2 to seal.According to EXPERIMENTAL DESIGN, during test, by the quick effuser of culture fluid 12, normal culture fluid is discharged, lead to
Crossing feed tube 15 and feed liquor water dropper 17 provides various nutritional labelings not enough culture fluid to cell, multiple dripping hole 18 dropping liquids, to the greatest extent
The extra interference that cell is produced may be reduced.There is provided the gas of different oxygen by air inlet pipe 13 and escape pipe 14 to cell
Body.
By rotating cap 22 rotate threaded rod 8 so that threaded rod 8 vertically feeds in screw shell 7, threaded rod 8 turn
Move to 9 one pressure of rotational sleeve, this pressure passes to pressured column 11 by support 10, thus the periphery to flexible slide glass 3
Pressurizeed in position so that flexible slide glass 3 produces symmetrical deformation, by controlling the amount of feeding of threaded rod 8, to different outer of cell
Boundary's tension force, is then gradually reduced pressure, simulation controlling decompression according to test requirements document.
After test is carried out to a certain extent, need by feed liquor water dropper 17 and go out the cooperation of liquid suction nozzle 19 gradually by low battalion
The culture fluid of foster component content is replaced by the culture fluid of normal nutrition composition.By the above-mentioned simulation to various conditions, can be right
The controlling decompression of cell carries out systemic experiment.After test completes operation, by flexible slide glass 3 together with cell attached thereto
24 take out together, can be observed under the microscope.
Claims (10)
1. a kind of assay device for cell controlling pressure relief model, including the culture dish with Packed lid (1) (2),
It is characterized in that: also include flexible slide glass (3), the bottom center position of described flexible slide glass (3) is provided with positioning ring foot
(4), the bottom centre position in described culture dish (2) is provided with positioning support base (5), and positioning ring foot (4) is enclosed within positioning
In support seat (5)；
The top surface surrounding positional symmetry of described flexible slide glass (3) is provided with stress location hole (6), on described lid (1)
Center is provided with the screw shell (7) of insertion lid, is provided with threaded rod (8) in screw shell (7), and threaded rod (8) is stretched
Enter the internal one end of culture dish (2) to be connected with one end of rotational sleeve (9), rotational sleeve (9) can turn with respect to threaded rod (8)
Dynamic, the other end of rotational sleeve (9) is fixed in the center of support (10), and the end of support (10) surrounding is provided with vertical
Pressured column (11), pressured column (11) withstands in pressurization location hole (6) and is used for the flexible slide glass (3) that bends；
Described culture dish (2) near bottom side wall on be communicated with the quick effuser of culture fluid (12)；
Air inlet pipe (13) and escape pipe (14) are communicated with described lid (1)；
Feed tube (15) and drain pipe (16) are also inserted with described lid (1).
2. assay device as claimed in claim 1 it is characterised in that: end in culture dish (2) for the described feed tube (15)
Portion is provided with the feed liquor water dropper (17) planar spiraling, and feed liquor water dropper (17) is evenly arranged with multiple dripping holes (18)；Institute
End in culture dish (2) for the drain pipe (16) stated be provided with planar spiral go out liquid suction nozzle (19), go out liquid suction nozzle
(19) it is evenly arranged with multiple imbibing holes (20) on.
3. assay device as claimed in claim 2 it is characterised in that: described feed liquor water dropper (17) and go out liquid suction nozzle (19) phase
Mutually be nested in approximately the same plane, described feed liquor water dropper (17) and go out liquid suction nozzle (19) center arrange a through hole
(21), threaded rod (8) passes through from through hole (21).
4. assay device as claimed in claim 1 it is characterised in that: described lid (1) and culture dish (2) are all using transparent
Material make.
5. assay device as claimed in claim 4 it is characterised in that: described transparent material be glass or transparent plastic.
6. assay device as claimed in claim 5 it is characterised in that: described transparent plastic be lucite.
7. assay device as claimed in claim 1 it is characterised in that: described flexible slide glass (3) adopts transparent plastics system
Become.
8. assay device as claimed in claim 1 it is characterised in that: the thickness of described flexible slide glass (3) be 0.2mm～
2mm.
9. assay device as claimed in claim 1 it is characterised in that: described threaded rod (8) stretches out on one end of lid (1)
It is provided with rotating cap (22).
10. assay device as claimed in claim 1 it is characterised in that: described stress location hole (6) be four, described
Support (10) is cross frame.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610809076.8A CN106353490B (en) | 2016-09-07 | 2016-09-07 | Test device for cell control decompression model |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610809076.8A CN106353490B (en) | 2016-09-07 | 2016-09-07 | Test device for cell control decompression model |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106353490A true CN106353490A (en) | 2017-01-25 |
| CN106353490B CN106353490B (en) | 2023-05-26 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610809076.8A Active CN106353490B (en) | 2016-09-07 | 2016-09-07 | Test device for cell control decompression model |
Country Status (1)
| Country | Link |
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| CN (1) | CN106353490B (en) |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2314343B (en) * | 1996-06-18 | 2000-08-23 | Liau Ming Yi | Method and apparatus for cultivating anchorage dependent monolayer cells |
| CN2818484Y (en) * | 2005-07-15 | 2006-09-20 | 中国人民解放军第四军医大学 | Cell tractive tension controller |
| JP2008092935A (en) * | 2006-10-13 | 2008-04-24 | Otake:Kk | Culturing apparatus in which pump function is provided in hermetically sealed culture container |
| CN201737949U (en) * | 2010-07-12 | 2011-02-09 | 中国人民解放军第三军医大学第一附属医院 | Cell culturing device capable of periodically converting positive pressure and negative pressure |
| CN201815216U (en) * | 2010-09-15 | 2011-05-04 | 李锦记（新会）食品有限公司 | Vacuum brewing material suction solid-gas separator |
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2016
- 2016-09-07 CN CN201610809076.8A patent/CN106353490B/en active Active
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| CN106353490B (en) | 2023-05-26 |
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