CN206132771U - A test device that is used for controlled decompression model of cell - Google Patents
A test device that is used for controlled decompression model of cell Download PDFInfo
- Publication number
- CN206132771U CN206132771U CN201621043761.6U CN201621043761U CN206132771U CN 206132771 U CN206132771 U CN 206132771U CN 201621043761 U CN201621043761 U CN 201621043761U CN 206132771 U CN206132771 U CN 206132771U
- Authority
- CN
- China
- Prior art keywords
- slide glass
- flexible slide
- culture dish
- assay device
- threaded rod
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 230000006837 decompression Effects 0.000 title abstract description 15
- 238000012360 testing method Methods 0.000 title abstract description 15
- 239000011521 glass Substances 0.000 claims abstract description 38
- 238000003556 assay Methods 0.000 claims description 18
- 239000007788 liquid Substances 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 239000012531 culture fluid Substances 0.000 claims description 14
- 229920003023 plastic Polymers 0.000 claims description 8
- 239000012780 transparent material Substances 0.000 claims description 6
- 229920005479 Lucite® Polymers 0.000 claims description 3
- 238000003780 insertion Methods 0.000 claims description 3
- 230000037431 insertion Effects 0.000 claims description 3
- 239000004926 polymethyl methacrylate Substances 0.000 claims description 3
- 238000005452 bending Methods 0.000 abstract description 3
- 230000008878 coupling Effects 0.000 abstract 1
- 238000010168 coupling process Methods 0.000 abstract 1
- 238000005859 coupling reaction Methods 0.000 abstract 1
- 238000010586 diagram Methods 0.000 description 6
- 210000002683 foot Anatomy 0.000 description 6
- 206010019196 Head injury Diseases 0.000 description 3
- 208000029028 brain injury Diseases 0.000 description 3
- 206010008118 cerebral infarction Diseases 0.000 description 3
- 208000026106 cerebrovascular disease Diseases 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 238000007428 craniotomy Methods 0.000 description 2
- 230000000991 decompressive effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000005213 imbibition Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 230000002980 postoperative effect Effects 0.000 description 2
- 238000004393 prognosis Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000004088 simulation Methods 0.000 description 2
- FGRBYDKOBBBPOI-UHFFFAOYSA-N 10,10-dioxo-2-[4-(N-phenylanilino)phenyl]thioxanthen-9-one Chemical compound O=C1c2ccccc2S(=O)(=O)c2ccc(cc12)-c1ccc(cc1)N(c1ccccc1)c1ccccc1 FGRBYDKOBBBPOI-UHFFFAOYSA-N 0.000 description 1
- 208000023442 Cephalocele Diseases 0.000 description 1
- 241000255925 Diptera Species 0.000 description 1
- 208000002403 Encephalocele Diseases 0.000 description 1
- AUYYCJSJGJYCDS-LBPRGKRZSA-N Thyrolar Chemical class IC1=CC(C[C@H](N)C(O)=O)=CC(I)=C1OC1=CC=C(O)C(I)=C1 AUYYCJSJGJYCDS-LBPRGKRZSA-N 0.000 description 1
- 208000030886 Traumatic Brain injury Diseases 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 239000005495 thyroid hormone Substances 0.000 description 1
- 229940036555 thyroid hormone Drugs 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
- 230000000472 traumatic effect Effects 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a test device that is used for controlled decompression model of cell, wherein, the bottom surface central point of flexible slide glass puts and is provided with the position circle enough, the culture dish in bottom central point put and be provided with the location support seat, the sufficient cover of position circle is on the location support seat, flexible slide glass the top surface all around symmetry be provided with the atress locating hole, the lid on central point put and be provided with the screw sleeve who has a perfect understanding the lid, install the threaded rod in the screw sleeve, the one end that the threaded rod stretched into the inside one end of culture dish and rotating sleeve links to each other, rotating sleeve can rotate for the threaded rod, rotating sleeve's other end rigid coupling is put at the central point of support, support tip all around is provided with vertical pressurization post, the pressurization post top flexible slide glass that is used for bending in the pressurization locating hole. The utility model discloses a flexible slide glass takes out in can following test device, the direct observation under instruments such as microscope of being convenient for, convenient and fast, easily operation.
Description
Technical field
This utility model belongs to brain injury field tests, is related to controlling decompression, and in particular to one kind is for cell control
The assay device of property pressure relief model.
Background technology
Up to 30%～60%, the prognosis for how reducing mortality rate and improving patient is levels of thyroid hormone in craniocerebral trauma patients mortality rate
The important topic that neurosurgical doctor pays special attention to.Standard large trauma craniotomy has obtained extensively should in such patient
With.
Decompressive craniectomy (decompressive craniectomuy, DC) is the intractable cranium for the treatment of severe traumatic head injury
The effective ways of high pressure patient.Controlling decompression in art is effectively reduced the correlation such as acute encephalocele in art, postoperative cerebral infarction simultaneously
Send out the incidence rate of disease.
Complicated by postoperative cerebral infarction is one of severe complication after craniotomy on patients with severe craniocerebral injury, is seen child is quite a few,
The mortality rate and disability rate of patient can substantially be increased, how discussion reduces severe cranial brain injury cerebral infarction incidence rate and improvement
The prognosis of patient has important research meaning.Using the retrospective comparative study operative treatment child heavy type cranium of controlling decompression in art
Brain injury patient, works well.
Based on above-mentioned clinical experience, controlling decompression method has clinically had more application, but in clinical practice
Being operated, still controlling decompression of the nobody to encephalic operation carries out science to the experience of more dependence medical personnel in journey
System experimentation, provides certain law and guides for clinic, also no in prior art to be tested for controlling decompression
Special purpose device so that research worker is difficult to the controlling decompression test of system.
The content of the invention
In view of the shortcomings of the prior art, the purpose of this utility model is, there is provided one kind subtracts for cell controlling
The assay device of pressing mold type, solves to be difficult to systematically ask for the technology of cell being controlled property decompression test in prior art
Topic.
In order to solve above-mentioned technical problem, this utility model is adopted the following technical scheme that and is achieved：
A kind of assay device for cell controlling pressure relief model, including the culture dish with Packed lid, also wraps
Flexible slide glass is included, the bottom center position of described flexible slide glass is provided with positioning ring foot, in the bottom in described culture dish
Heart position is provided with positioning support base, and centring ring foot cover is on positioning support base；
The top surface surrounding positional symmetry of described flexible slide glass is provided with stress location hole, the centre bit on described lid
The screw shell for being equipped with insertion lid is installed, threaded rod is installed in screw shell, threaded rod stretches into the one end inside culture dish
It is connected with one end of rotational sleeve, rotational sleeve can be rotated relative to threaded rod, and the other end of rotational sleeve is fixed in support
Center, the end of support surrounding is provided with vertical pressured column, and pressured column withstands on soft for bending in pressurization location hole
Property slide glass；
Described culture dish is communicated with the quick effuser of culture fluid on the side wall of bottom；
Air inlet pipe and escape pipe are communicated with described lid；
Feed tube and drain pipe are also inserted with described lid.
This utility model also has following distinguishing feature：
End of the described feed tube in culture dish is provided with the feed liquor water dropper for planar spiraling, on feed liquor water dropper
It is even to be provided with multiple dripping holes；End of the described drain pipe in culture dish be provided with planar spiral go out liquid suction nozzle,
Go out on liquid suction nozzle and be evenly arranged with multiple imbibing holes.
Described feed liquor water dropper is mutually nested with liquid suction nozzle is gone out in approximately the same plane, and described feed liquor water dropper is inhaled with liquid is gone out
The center of head arranges a through hole, and threaded rod is passed through from through hole.
Described lid and culture dish is made using transparent material.
Described transparent material is glass or transparent plastic.
Described transparent plastic is lucite.
Described flexible slide glass is made using transparent plastics.
The thickness of described flexible slide glass is 0.2mm～2mm.
Described threaded rod stretches out and be provided with one end of lid rotating cap.
Described stress location hole is four, and described support is cross frame.
This utility model compared with prior art, has the following technical effect that：
Assay device of the present utility model, can set up the test model of a set of controlling decompression, by changing for cell
Become nutrient composition content, oxygen concentration and the external tension to cell applying of culture fluid, the controlling of cell can be subtracted
Pressure carries out the test of system, is easy to experimenter to find the controlling reduced pressure of optimum, provides beneficial for clinical practice operation
Guidance.
Feed liquor water dropper of the present utility model spirals with the plane that liquid suction nozzle is similar mosquito incense shape is gone out, multiple dripping holes and imbibition
Hole so that can be closer to cell when dropping liquid and imbibition, it is to avoid the unwanted external interference of test institute is caused to cell.
Flexible slide glass of the present utility model can take out from assay device, be easy to directly be seen under the instruments such as microscope
Examine, it is convenient and swift, it is easy to operate.
Description of the drawings
Fig. 1 is overall structure diagram of the present utility model.
Fig. 2 is feed liquor water dropper and goes out the surface structure schematic diagram of drop head.
Fig. 3 is feed liquor water dropper and goes out the lower surface configuration schematic diagram of drop head.
Fig. 4 is the structural representation of support.
Fig. 5 is the top surface structure schematic diagram of flexible slide glass.
Fig. 6 is the bottom surface structure schematic diagram of flexible slide glass.
Fig. 7 is use state schematic diagram of the present utility model.
The implication of each label is in figure：1- lids, 2- culture dishs, 3- flexibility slide glasses, 4- positioning ring foots, 5- positioning is supported
Seat, 6- stress location holes, 7- screw shells, 8- threaded rods, 9- rotational sleeves, 10- supports, 11- pressured columns, 12- culture fluid is fast
Fast effuser, 13- air inlet pipe, 14- escape pipes, 15- feed tubes, 16- drain pipes, 17- feed liquor water droppers, 18- dripping holes, 19- goes out
Liquid suction nozzle, 20- imbibing holes, 21- through holes, 22- rotating caps, 23- culture fluid, 24- cells.
With reference to embodiments explanation is further explained in detail to particular content of the present utility model.
Specific embodiment
Specific embodiment of the utility model given below, it should be noted that this utility model is not limited to following tool
Body embodiment, all equivalents done on the basis of technical scheme each fall within protection domain of the present utility model.
Embodiment：
Above-mentioned technical proposal is deferred to, as shown in Figures 1 to 7, the present embodiment provides a kind of for cell controlling decompression mould
The assay device of type, including the culture dish 2 with Packed lid 1, also including flexible slide glass 3, the bottom of described flexible slide glass 3
Face center is provided with positioning ring foot 4, and the bottom centre position in described culture dish 2 is provided with positioning support base 5, positioning
Ring foot 4 is enclosed within positioning support base 5；
The top surface surrounding positional symmetry of described flexible slide glass 3 is provided with stress location hole 6, on described lid 1 in
Heart position is provided with the screw shell 7 of insertion lid, screw shell 7 and is provided with threaded rod 8, and threaded rod 8 is stretched in culture dish 2
The one end in portion is connected with one end of rotational sleeve 9, and rotational sleeve 9 can be rotated relative to threaded rod 8, rotational sleeve 9 it is another
End is fixed in the center of support 10, and the end of the surrounding of support 10 is provided with vertical pressured column 11, and pressured column 11 withstands on and adds
For the flexible slide glass 3 that bends in pressure location hole 6；
Described culture dish 2 is communicated with the quick effuser 12 of culture fluid on the side wall of bottom；
Air inlet pipe 13 and escape pipe 14 are communicated with described lid 1；
Feed tube 15 and drain pipe 16 are also inserted with described lid 1.
End of the described feed tube 15 in culture dish 2 is provided with the feed liquor water dropper 17 for planar spiraling, and enters drop
Multiple dripping holes 18 are evenly arranged with 17；End of the described drain pipe 16 in culture dish 2 is provided with planar disk
What is revolved goes out liquid suction nozzle 19, goes out on liquid suction nozzle 19 and is evenly arranged with multiple imbibing holes 20.
Feed liquor water dropper 17 is mutually nested with liquid suction nozzle 19 is gone out in approximately the same plane, is easy to have better access to flexible slide glass 3,
And do not affect cell 23.Described feed liquor water dropper 17 arranges a through hole 21, threaded rod 8 with the center for going out liquid suction nozzle 19
Pass through from through hole 21.
Lid 1 and culture dish 2 are made using transparent material, are easy to observation, and transparent material is glass or transparent modeling
Material, transparent plastic is preferably lucite.
Flexible slide glass 3 is made using transparent plastics, meets flexible bending and the demand recovered, and can be in microscope
It is lower directly to observe.The thickness of flexible slide glass 3 is 0.2mm～2mm, can be according to flexible load of some row thickness of test Demand Design
Piece 3, for simulating different extraneous expansionary forces, is convenient for some row controlling decompression tests.
Threaded rod 8 stretches out and rotating cap 22 is provided with one end of lid 1, for selecting threaded rod 8, and then extrudes flexible load
Piece 3.
Stress location hole 6 is four, and described support 10 is cross frame, is corresponded, and is applied to the surrounding of flexible slide glass 3
Uniform pressure, location hole 6 can also be replaced with annular positioning groove, and pressured column 11 could alternatively be into corresponding annular protrusion,
So that pressurized uniform.
Culture dish 2 is used to hold culture fluid 23, and attached cell 24 is used on flexible slide glass 3.
The shape of flexible slide glass 3 as needed selecting, circular.
The course of work of the present utility model is as described below：
When assay device of the present utility model is used, flexible slide glass 3 is placed in culture dish 2, by positioning the He of ring foot 4
Location and installation is realized in the cooperation of positioning support base 5.Normal culture fluid is poured in culture dish 2, is then cultivated in culture fluid
Cell, cell attachment closes the lid 1 on flexible slide glass 3 so that pressured column 11 coordinates but do not produce pressure with stress location hole 6
Power so that culture dish 2 is sealed.According to EXPERIMENTAL DESIGN, during test, normal culture fluid is arranged by the quick effuser 12 of culture fluid
Go out, provide various nutritional labelings not enough culture fluid to cell by feed tube 15 and feed liquor water dropper 17, multiple dripping holes 18 drip
Liquid, reduces as far as possible the extra interference produced to cell.Different oxygen is provided by air inlet pipe 13 and escape pipe 14 to cell
Gas.
By rotating cap 22 rotate threaded rod 8 so that threaded rod 8 vertically feeds in screw shell 7, threaded rod 8 turn
Move and give rotational sleeve 9 one pressure, the pressure passes to pressured column 11 by support 10, so as to the periphery to flexible slide glass 3
Pressurizeed position so that flexible slide glass 3 produces symmetrical deformation, by the amount of feeding for controlling threaded rod 8, give cell different outer
Boundary's tension force, is then gradually reduced pressure, simulation controlling decompression according to test requirements document.
After test is carried out to a certain extent, need by feed liquor water dropper 17 and go out the cooperation of liquid suction nozzle 19 gradually by low battalion
The culture fluid of foster component content is replaced by the culture fluid of normal nutrition composition.By the above-mentioned simulation to various conditions, can be right
The controlling decompression of cell carries out systemic experiment.Test is completed after operation, by flexible slide glass 3 together with cell attached thereto
24 take out together, can be observed under the microscope.
Claims (10)
1. a kind of assay device for cell controlling pressure relief model, including the culture dish with Packed lid (1) (2),
It is characterized in that：Also include flexible slide glass (3), the bottom center position of described flexible slide glass (3) is provided with positioning ring foot
(4), the bottom centre position in described culture dish (2) is provided with positioning support base (5), and positioning ring foot (4) is enclosed within positioning
In support seat (5)；
The top surface surrounding positional symmetry of described flexible slide glass (3) is provided with stress location hole (6), on described lid (1)
Center is provided with the screw shell (7) of insertion lid, screw shell (7) and is provided with threaded rod (8), and threaded rod (8) is stretched
Enter the internal one end of culture dish (2) to be connected with one end of rotational sleeve (9), rotational sleeve (9) can turn relative to threaded rod (8)
Dynamic, the other end of rotational sleeve (9) is fixed in the center of support (10), and the end of support (10) surrounding is provided with vertical
Pressured column (11), pressured column (11) is withstood in pressurization location hole (6) for the flexible slide glass (3) that bends；
Described culture dish (2) is communicated with the quick effuser of culture fluid (12) on the side wall of bottom；
Air inlet pipe (13) and escape pipe (14) are communicated with described lid (1)；
Feed tube (15) and drain pipe (16) are also inserted with described lid (1).
2. assay device as claimed in claim 1, it is characterised in that：End of the described feed tube (15) in culture dish (2)
Portion is provided with the feed liquor water dropper (17) for planar spiraling, and multiple dripping holes (18) are evenly arranged with feed liquor water dropper (17)；Institute
End of the drain pipe (16) stated in culture dish (2) be provided with planar spiral go out liquid suction nozzle (19), go out liquid suction nozzle
(19) multiple imbibing holes (20) are evenly arranged with.
3. assay device as claimed in claim 2, it is characterised in that：Described feed liquor water dropper (17) and go out liquid suction nozzle (19) phase
Mutually it is nested in approximately the same plane, described feed liquor water dropper (17) and goes out the center of liquid suction nozzle (19) through hole is set
(21), threaded rod (8) is passed through from through hole (21).
4. assay device as claimed in claim 1, it is characterised in that：Described lid (1) and culture dish (2) adopts transparent
Material make.
5. assay device as claimed in claim 4, it is characterised in that：Described transparent material is glass or transparent plastic.
6. assay device as claimed in claim 5, it is characterised in that：Described transparent plastic is lucite.
7. assay device as claimed in claim 1, it is characterised in that：Described flexible slide glass (3) is using transparent plastics system
Into.
8. assay device as claimed in claim 1, it is characterised in that：The thickness of described flexible slide glass (3) be 0.2mm～
2mm。
9. assay device as claimed in claim 1, it is characterised in that：Described threaded rod (8) is stretched out on one end of lid (1)
It is provided with rotating cap (22).
10. assay device as claimed in claim 1, it is characterised in that：Described stress location hole (6) is four, described
Support (10) is cross frame.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621043761.6U CN206132771U (en) | 2016-09-07 | 2016-09-07 | A test device that is used for controlled decompression model of cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621043761.6U CN206132771U (en) | 2016-09-07 | 2016-09-07 | A test device that is used for controlled decompression model of cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206132771U true CN206132771U (en) | 2017-04-26 |
Family
ID=58568943
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621043761.6U Expired - Fee Related CN206132771U (en) | 2016-09-07 | 2016-09-07 | A test device that is used for controlled decompression model of cell |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206132771U (en) |
-
2016
- 2016-09-07 CN CN201621043761.6U patent/CN206132771U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170426 Termination date: 20180907 |