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CA2502981A1 - Transgenic glyphosate tolerant sugar beet event h7-1 - Google Patents

Transgenic glyphosate tolerant sugar beet event h7-1 Download PDF

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Publication number
CA2502981A1
CA2502981A1 CA002502981A CA2502981A CA2502981A1 CA 2502981 A1 CA2502981 A1 CA 2502981A1 CA 002502981 A CA002502981 A CA 002502981A CA 2502981 A CA2502981 A CA 2502981A CA 2502981 A1 CA2502981 A1 CA 2502981A1
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CA
Canada
Prior art keywords
seq
nucleotide sequence
primer
sugar beet
parts
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002502981A
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French (fr)
Other versions
CA2502981C (en
Inventor
Josef Kraus
Elke Sauerbrey
Reinhard Nehls
Andreas Loock
Rudolf Jansen
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KWS SAAT SE and Co KGaA
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Individual
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Priority claimed from US10/376,763 external-priority patent/US7335816B2/en
Application filed by Individual filed Critical Individual
Publication of CA2502981A1 publication Critical patent/CA2502981A1/en
Application granted granted Critical
Publication of CA2502981C publication Critical patent/CA2502981C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention relates to sugar beet-like glyphosate tolerant vegetables, vegetable material and seeds. The aim of said invention is to prepare a transgenic event for a sugar beet having a high-degree tolerance with respect to glyphosate and preserving other important agronomic properties in terms of growth, yield, quality and a pathogenic resistance, etc.

Claims (14)

1. Glyphosate tolerant sugar beet plant, characterized in that a) the sugar beet plant is obtained from seed deposited with the NCIMB and having the accession number NCIMB
41158 or NCIMB 41159, and/or b) a DNA fragment of between 630-700 bp, preferably 664 bp, can be amplified from the genomic DNA of said sugar beet plant, parts or seeds thereof, using polymerase chain reaction with a first primer having the nucleotide sequence of SEQ ID NO: 1 and a second primer having the nucleotide sequence of SEQ ID NO: 2, and/or c) a DNA fragment of 3500-3900, preferably 3706 bp, can be amplified from the genomic DNA of said sugar beet plant, parts or seeds thereof, using polymerase chain reaction with a first primer having the nucleotide sequence of SEQ ID NO: 3 and a second primer having the nucleotide sequence of SEQ ID NO: 4, and/or d) a DNA fragment of 270-300 bp, preferably 288 bp, can be amplified from the genomic DNA of said sugar beet plant, parts or seeds thereof, using polymerase chain reaction with a first primer having the nucleotide sequence of SEQ ID NO: 7 and a second primer having the nucleotide sequence of SEQ ID NO: 8, and/or e) a DNA fragment of 710-790 bp, preferably 751 bp, can be amplified from the genomic DNA of said sugar beet plant, parts or seeds thereof, using polymerase chain reaction with a first primer having the nucleotide sequence of SEQ ID NO: 9 and a second primer having the nucleotide sequence of SEQ ID NO: 10, and/or f) a DNA fragment of 990-1100 bp, preferably 1042 bp, can be amplified from the genomic DNA of said sugar beet plant, parts or seeds thereof, using polymerase chain reaction with a first primer having the nucleotide sequence of SEQ ID NO: 14 and a second primer having the nucleotide sequence of SEQ ID NO: 16.
2. Glyphosate tolerant sugar beet plant according to claim 1, characterized in that said 3706 by DNA fragment has the nucleotide sequence of SEQ ID NO: 6 or shows at least 95 %, preferably at least 99 %, more preferably at least 99,9 %, identity with the nucleotide sequence of SEQ ID
NO: 6.
3. Glyphosate tolerant sugar beet plant according to claim 1, characterized in that said 664 by DNA fragment has the nucleotide sequence of SEQ ID NO: 13 or shows at least 95 %, preferably at least 99 %, more preferably at least 99,9 %, identity with the nucleotide sequence of SEQ ID
NO: 13.
4. Glyphosate tolerant sugar beet plant according to claim 1, characterized in that said 288 by DNA fragment has the nucleotide sequence of SEQ ID NO: 11 or shows at least 95 %, preferably at least 99 %, more preferably at least 99,9 %, identity with the nucleotide sequence of SEQ ID
NO: 11.
5. Glyphosate tolerant sugar beet plant according to claim 1, characterized in that said 751 by DNA fragment has the nucleotide sequence of SEQ ID NO: 12 or shows at least 95 %, preferably at least 99 %, more preferably at least 99,9 %, identity with the nucleotide sequence of SEQ ID
NO: 12.
6. Glyphosate tolerant sugar beet plant according to claim 1, characterized in that said 1042 by DNA fragment has the nucleotide sequence of SEQ ID NO: 17 or shows at least 95 %, preferably at least 99 %, more preferably at least 99,9 %, identity with the nucleotide sequence of SEQ ID NO: 17.
7. Seed of a plant according to one of claims 1 to 6.
8. Cell, tissue or parts from the plant according to one of claims 1 to 6.
9. Seed deposited with the NCIMB and having the accession number NCIMB 41158 or NCIMB 41159.
10. Plant, and parts thereof, obtained from seed according to claim 9.
11. Method for identification of a glyphosate tolerant sugar beet plant, characterized in that the method comprises the step (s) of a) amplifying a DNA fragment of between 630-700 bp, preferably 664 bp, from the genomic DNA of said sugar beet plant, parts or seeds thereof, using polymerase chain reaction with a first primer having the nucleotide sequence of SEQ ID NO: 1 and a second primer having the nucleotide sequence of SEQ ID NO: 2, and/or b) amplifying a DNA fragment of 3500-3900, preferably 3706 bp, from the genomic DNA of said sugar beet plant, parts or seeds thereof, using polymerase chain reaction with a first primer having the nucleotide sequence of SEQ
ID NO: 3 and a second primer having the nucleotide sequence of SEQ ID NO: 4, and/or c) amplifying a DNA fragment of 270-300 bp, preferably 288 bp, from the genomic DNA of said sugar beet plant, parts or seeds thereof, using polymerase chain reaction with a first primer having the nucleotide sequence of SEQ
ID NO: 7 and a second primer having the nucleotide sequence of SEQ ID NO: 8, and/or d) amplifying a DNA fragment of 710-790 bp, preferably 751 bp, from the genomic DNA of said sugar beet plant, parts or seeds thereof, using polymerase chain reaction with a first primer having the nucleotide sequence of SEQ
ID NO: 9 and a second primer having the nucleotide sequence of SEQ ID NO: 10, and/or e) amplifying a DNA fragment of 990-1100 bp, preferably 1042 bp, from the genomic DNA of said sugar beet plant, parts or seeds thereof, using polymerase chain reaction with a first primer having the nucleotide sequence of SEQ
ID NO: 14 and a second primer having the nucleotide sequence of SEQ ID NO: 16.
12.Test kit for identifying a transgenic glyphosate tolerant sugar beet plant, its cells, tissue or parts, comprising at least one primer pair with a first and a second primer for polymerase chain reaction, the first primer recognizing a sequence within the foreign DNA
incorporated into the genome of said plant, and the second primer recognizing a sequence within the 3' or 5' flanking regions of said DNA, whereby the plant is a plant according to one of claims 1 to 6, or 10.
13. Test kit for identifying a transgenic glyphosate tolerant sugar beet plant, its cells, tissue or parts, comprising at least one primer pair with a first and a second primer for polymerase chain reaction, the first primer recognizing a sequence within the foreign DNA
incorporated into the genome of said plant, and the second primer recognizing a sequence within the 3' or 5' flanking regions of said DNA, characterized in that a) the first primer has the nucleotide sequence of SEQ ID
NO: 1 and the second primer has the nucleotide sequence of SEQ ID NO: 2, and/or b) the first primer has the nucleotide sequence of SEQ ID
NO: 7 and the second primer has the nucleotide sequence of SEQ ID NO: 8, and/or c) the first primer has the nucleotide sequence of SEQ ID
NO: 9 and the second primer has the nucleotide sequence of SEQ ID NO: 10, and/or d) the first primer has the nucleotide sequence of SEQ ID
NO: 14 and the second primer has the nucleotide sequence of SEQ ID NO: 16.
14. Test kit according to claim 12 or 13, characterized in that the first and second primer recognizes a nucleotide sequence which forms part of the nucleotide sequence of SEQ ID NO: 5.
CA2502981A 2003-02-20 2004-02-17 Transgenic glyphosate tolerant sugar beet event h7-1 Expired - Lifetime CA2502981C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP03003866.5 2003-02-20
EP03003866 2003-02-20
US10/376,763 US7335816B2 (en) 2003-02-28 2003-02-28 Glyphosate tolerant sugar beet
US10/376,763 2003-02-28
PCT/EP2004/001469 WO2004074492A1 (en) 2003-02-20 2004-02-17 Glyphosate tolerant sugar beet

Publications (2)

Publication Number Publication Date
CA2502981A1 true CA2502981A1 (en) 2004-09-02
CA2502981C CA2502981C (en) 2012-09-11

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA2502981A Expired - Lifetime CA2502981C (en) 2003-02-20 2004-02-17 Transgenic glyphosate tolerant sugar beet event h7-1

Country Status (12)

Country Link
EP (1) EP1597373B1 (en)
JP (1) JP5586122B2 (en)
CN (1) CN102391988A (en)
CA (1) CA2502981C (en)
DK (1) DK1597373T3 (en)
EA (1) EA011923B1 (en)
ES (1) ES2391090T3 (en)
PL (1) PL214713B1 (en)
PT (1) PT1597373E (en)
RS (1) RS53441B (en)
SI (1) SI1597373T1 (en)
WO (1) WO2004074492A1 (en)

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