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MX365435B - Metodos de modificacion de celulas huesped. - Google Patents

Metodos de modificacion de celulas huesped.

Info

Publication number
MX365435B
MX365435B MX2015004649A MX2015004649A MX365435B MX 365435 B MX365435 B MX 365435B MX 2015004649 A MX2015004649 A MX 2015004649A MX 2015004649 A MX2015004649 A MX 2015004649A MX 365435 B MX365435 B MX 365435B
Authority
MX
Mexico
Prior art keywords
methods
host cell
cell modification
host cells
nucleic acid
Prior art date
Application number
MX2015004649A
Other languages
English (en)
Other versions
MX2015004649A (es
Inventor
Wacker Michael
Kowarik Michael
Fernandez Fabiana
Original Assignee
Glaxosmithkline Biologicals Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glaxosmithkline Biologicals Sa filed Critical Glaxosmithkline Biologicals Sa
Publication of MX2015004649A publication Critical patent/MX2015004649A/es
Publication of MX365435B publication Critical patent/MX365435B/es

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    • C—CHEMISTRY; METALLURGY
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/40—Systems of functionally co-operating vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

La presente invención se refiere a métodos novedosos de inserción de secuencias de ácido nucleico en células huésped. En la presente también se describen células huésped genéticamente estables que comprenden secuencias de ácido nucleico insertadas, y métodos para utilizar tales células huésped en la generación de proteínas.
MX2015004649A 2012-10-12 2013-10-11 Metodos de modificacion de celulas huesped. MX365435B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261713281P 2012-10-12 2012-10-12
PCT/EP2013/071328 WO2014057109A1 (en) 2012-10-12 2013-10-11 Methods of host cell modification

Publications (2)

Publication Number Publication Date
MX2015004649A MX2015004649A (es) 2016-01-12
MX365435B true MX365435B (es) 2019-06-03

Family

ID=49326691

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015004649A MX365435B (es) 2012-10-12 2013-10-11 Metodos de modificacion de celulas huesped.

Country Status (16)

Country Link
US (2) US20150267207A1 (es)
EP (2) EP3527663B1 (es)
JP (1) JP6329554B2 (es)
KR (1) KR20150068998A (es)
CN (2) CN110423716A (es)
AU (1) AU2013328615B2 (es)
BR (1) BR112015008052A2 (es)
CA (1) CA2887133C (es)
EA (1) EA201590488A1 (es)
ES (2) ES2905107T3 (es)
IL (1) IL238024A0 (es)
MX (1) MX365435B (es)
SG (2) SG10201800777PA (es)
TR (1) TR201904022T4 (es)
WO (1) WO2014057109A1 (es)
ZA (1) ZA201502433B (es)

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US9849169B2 (en) 2013-01-17 2017-12-26 Arsanis Biosciences Gmbh MDR E. coli specific antibody
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JP2016533719A (ja) 2013-10-11 2016-11-04 グリコヴァキシン アーゲー 宿主細胞改変方法
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JP6682451B2 (ja) * 2014-04-17 2020-04-15 グラクソスミスクライン バイオロジカルズ ソシエテ アノニム 改変型宿主細胞およびその使用
CN107429241A (zh) * 2014-08-14 2017-12-01 北京百奥赛图基因生物技术有限公司 Dna敲入系统
AU2016224006B8 (en) 2015-02-26 2020-03-19 Vaxnewmo Llc Acinetobacter O-oligosaccharyltransferases and uses thereof
TWI715617B (zh) 2015-08-24 2021-01-11 比利時商葛蘭素史密斯克藍生物品公司 對抗腸道外病原性大腸桿菌之免疫保護之方法及組合物
GB201518668D0 (en) 2015-10-21 2015-12-02 Glaxosmithkline Biolog Sa Immunogenic Comosition
GB201610599D0 (en) 2016-06-17 2016-08-03 Glaxosmithkline Biologicals Sa Immunogenic Composition
AR109621A1 (es) 2016-10-24 2018-12-26 Janssen Pharmaceuticals Inc Formulaciones de vacunas contra glucoconjugados de expec
CN106801029B (zh) * 2017-01-22 2020-05-01 山东大学 一株重组大肠杆菌及其在制备抗志贺氏菌的糖疫苗中的应用
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GB201712678D0 (en) 2017-08-07 2017-09-20 Glaxosmithkline Biologicals Sa Process for the manipulation of nucleic acids
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EP3806881A4 (en) 2018-06-16 2022-07-27 Vaxnewmo Llc GLYCOSYLATED COMP-PILIN VARIANTS, METHOD OF MANUFACTURE AND USES THEREOF
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TWI751513B (zh) 2019-03-18 2022-01-01 美商詹森藥物公司 E. coli O-抗原多醣生物結合物、其製備方法及其使用方法
EP3770269A1 (en) 2019-07-23 2021-01-27 GlaxoSmithKline Biologicals S.A. Quantification of bioconjugate glycosylation
BR112022000969A2 (pt) 2019-08-09 2022-06-14 Glaxosmithkline Biologicals Sa Enzimas pglb oligossacariltransferase mutadas
JP2023530154A (ja) * 2020-06-18 2023-07-13 グラクソスミスクライン バイオロジカルズ ソシエテ アノニム 赤痢菌４価（Ｓｈｉｇｅｌｌａ４Ｖ）バイオコンジュゲート
EP4171625A2 (en) 2020-06-25 2023-05-03 GlaxoSmithKline Biologicals SA Klebsiella pneumoniae o-antigen vaccine
CN119454928A (zh) 2020-09-17 2025-02-18 杨森制药公司 多价疫苗组合物及其用途
US12138302B2 (en) 2020-10-27 2024-11-12 Pfizer Inc. Escherichia coli compositions and methods thereof
JP2022099419A (ja) * 2020-12-23 2022-07-05 トヨタ自動車株式会社 形質転換用プラスミド及びこれを用いた形質転換体の製造方法、並びに形質転換方法
EP4291673A2 (en) 2021-02-11 2023-12-20 GlaxoSmithKline Biologicals SA Mutated pglb oligosaccharyltransferase enzymes
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CN113528410A (zh) * 2021-06-23 2021-10-22 云舟生物科技（广州）有限公司 一种用于快速高效诱导表达外源蛋白的大肠杆菌工程菌及其制备方法与应用
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Also Published As

Publication number Publication date
AU2013328615A1 (en) 2015-04-30
ES2905107T3 (es) 2022-04-07
EP2906681B1 (en) 2019-01-09
IL238024A0 (en) 2015-05-31
KR20150068998A (ko) 2015-06-22
US20190218561A1 (en) 2019-07-18
CA2887133A1 (en) 2014-04-17
SG11201502638YA (en) 2015-05-28
US20150267207A1 (en) 2015-09-24
AU2013328615B2 (en) 2017-04-20
CN110423716A (zh) 2019-11-08
ES2720040T3 (es) 2019-07-17
MX2015004649A (es) 2016-01-12
EA201590488A1 (ru) 2015-09-30
JP6329554B2 (ja) 2018-05-23
EP2906681A1 (en) 2015-08-19
CN104837984A (zh) 2015-08-12
JP2015533497A (ja) 2015-11-26
EP3527663B1 (en) 2021-12-01
WO2014057109A1 (en) 2014-04-17
ZA201502433B (en) 2017-11-29
CA2887133C (en) 2022-05-03
TR201904022T4 (tr) 2019-04-22
BR112015008052A2 (pt) 2017-11-28
EP3527663A1 (en) 2019-08-21
SG10201800777PA (en) 2018-02-27

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