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MX350713B - Variantes de subtilisina y polinucleotidos que las codifican. - Google Patents

Variantes de subtilisina y polinucleotidos que las codifican.

Info

Publication number
MX350713B
MX350713B MX2014009711A MX2014009711A MX350713B MX 350713 B MX350713 B MX 350713B MX 2014009711 A MX2014009711 A MX 2014009711A MX 2014009711 A MX2014009711 A MX 2014009711A MX 350713 B MX350713 B MX 350713B
Authority
MX
Mexico
Prior art keywords
polynucleotides encoding
subtilisin variants
variants
encoding same
subtilisin
Prior art date
Application number
MX2014009711A
Other languages
English (en)
Other versions
MX2014009711A (es
Inventor
Besenmatter Werner
Malten Marco
Benie Astrid
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Publication of MX2014009711A publication Critical patent/MX2014009711A/es
Publication of MX350713B publication Critical patent/MX350713B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente invención se refiere a una variante de subtilisina que tiene actividad proteasa, caracterizada porque comprende las sustituciones que corresponden a Y217X y N218Z de SEQ ID NO: 2, en donde X y Z se seleccionan del grupo que consiste de K, H, R, D, E, S y T, en donde cuando X es ya sea K, h o R entonces Z también se selecciona de K, H o R, y en donde cuando X es ya sea D, E, S o T, entonces Z también se selecciona de D, E, S o T, en donde la variante tiene un rendimiento de lavado mejorado en manchas de huevo en comparación con la proteasa con SEQ ID NO: 2, y en donde la variante tiene al menos 85% de identidad de secuencia respecto del polipéptido maduro de SEQ ID NO: 2.
MX2014009711A 2012-02-17 2013-02-14 Variantes de subtilisina y polinucleotidos que las codifican. MX350713B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP12155956 2012-02-17
PCT/EP2013/052964 WO2013120948A1 (en) 2012-02-17 2013-02-14 Subtilisin variants and polynucleotides encoding same

Publications (2)

Publication Number Publication Date
MX2014009711A MX2014009711A (es) 2014-09-08
MX350713B true MX350713B (es) 2017-09-14

Family

ID=47754445

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014009711A MX350713B (es) 2012-02-17 2013-02-14 Variantes de subtilisina y polinucleotidos que las codifican.

Country Status (6)

Country Link
US (1) US10093911B2 (es)
EP (1) EP2814956B1 (es)
JP (1) JP2015509364A (es)
CN (1) CN104114698A (es)
MX (1) MX350713B (es)
WO (1) WO2013120948A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170114091A1 (en) * 2014-03-28 2017-04-27 Novozymes A/S Resolubilization of protein crystals at low ph
CN106661566A (zh) 2014-07-04 2017-05-10 诺维信公司 枯草杆菌酶变体以及编码它们的多核苷酸
EP3359634A1 (en) * 2015-10-09 2018-08-15 Novozymes A/S Laundry method, use of polypeptide and detergent composition
FR3047488B1 (fr) 2016-02-05 2020-02-28 Laboratoires Anios Compositions detergentes pour le nettoyage dans l'industrie cosmetique et pharmaceutique.
WO2018118917A1 (en) * 2016-12-21 2018-06-28 Danisco Us Inc. Protease variants and uses thereof
CN119286828B (zh) * 2024-12-11 2025-02-25 四川大学 一种中性蛋白酶突变体及其应用

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