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MX337919B - Polipeptidos que tienen actividad de celobiohidrolasa y polinucleotidos que codifican los mismos. - Google Patents

Polipeptidos que tienen actividad de celobiohidrolasa y polinucleotidos que codifican los mismos.

Info

Publication number
MX337919B
MX337919B MX2013008095A MX2013008095A MX337919B MX 337919 B MX337919 B MX 337919B MX 2013008095 A MX2013008095 A MX 2013008095A MX 2013008095 A MX2013008095 A MX 2013008095A MX 337919 B MX337919 B MX 337919B
Authority
MX
Mexico
Prior art keywords
polypeptides
polynucleotides encoding
cellobiohydrolase activity
encoding same
relates
Prior art date
Application number
MX2013008095A
Other languages
English (en)
Other versions
MX2013008095A (es
Inventor
Nikolaj Spodsberg
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Publication of MX2013008095A publication Critical patent/MX2013008095A/es
Publication of MX337919B publication Critical patent/MX337919B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención se refiere a una construcción de ácido nucleico caracterizada porque comprende un polinucleótido que codifica para un polipéptido que tiene actividad de celobiohidrolasa, en donde el polinucleótido está unido operativamente a una o más secuencias de control heterólogas que dirigen la producción del polipéptido en un anfitrión de expresión, y en donde el polipéptido se selecciona de: (a) un polipéptido que tiene al menos 95% de identidad de secuencia con los aminoácidos 19 a 464s de SEQ ID NO:2, (b) un polipéptido codificado por un polinucleótido que hibrida en condiciones de severidad muy alta con (i) nucleótidos 55 a de 1895 de SEQ ID NO: 1, (ii) la secuencia de ADNc del mismo, o (iii) el complemento de longitud completa de (i) o (ii), en donde las condiciones de severidad muy alta se definen como prehibridación a 42°C en 5XSSPE, 0.3% de SDS, 200 µg/ml de ADN de esperma de salmón cortado y desnaturalizado, y 50% de formamida y lavado tres veces cada una durante 15 minutos usando 2xSSC,0.2% SDS a 70°C; y (c) un polipéptido codificado por un polinucleótido que tiene al menos 95% de identidad de secuencia con los nucleótidos 55 a 1895 de SEQ ID NO: 1; o la secuencia de cDNA del mismo.
MX2013008095A 2011-01-26 2012-01-26 Polipeptidos que tienen actividad de celobiohidrolasa y polinucleotidos que codifican los mismos. MX337919B (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP11152252 2011-01-26
EP11250698 2011-08-04
EP11191773 2011-12-02
PCT/US2012/022653 WO2012103288A1 (en) 2011-01-26 2012-01-26 Polypeptides having cellobiohydrolase activity and polynucleotides encoding same

Publications (2)

Publication Number Publication Date
MX2013008095A MX2013008095A (es) 2013-10-03
MX337919B true MX337919B (es) 2016-03-28

Family

ID=46001705

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013008095A MX337919B (es) 2011-01-26 2012-01-26 Polipeptidos que tienen actividad de celobiohidrolasa y polinucleotidos que codifican los mismos.

Country Status (7)

Country Link
US (2) US9080161B2 (es)
EP (1) EP2668270B1 (es)
CN (1) CN103517986B (es)
BR (1) BR112013018695B1 (es)
DK (1) DK2668270T3 (es)
MX (1) MX337919B (es)
WO (1) WO2012103288A1 (es)

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US20150368629A1 (en) 2015-12-24
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MX2013008095A (es) 2013-10-03
US20130288299A1 (en) 2013-10-31

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