CN112970760A - Ternary compound bactericide and application thereof in prevention and treatment of wheat stem basal rot - Google Patents
Ternary compound bactericide and application thereof in prevention and treatment of wheat stem basal rot Download PDFInfo
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- CN112970760A CN112970760A CN202110209528.XA CN202110209528A CN112970760A CN 112970760 A CN112970760 A CN 112970760A CN 202110209528 A CN202110209528 A CN 202110209528A CN 112970760 A CN112970760 A CN 112970760A
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- 241000209140 Triticum Species 0.000 title claims abstract description 54
- 235000021307 Triticum Nutrition 0.000 title claims abstract description 52
- 150000001875 compounds Chemical class 0.000 title claims abstract description 36
- 230000002265 prevention Effects 0.000 title claims abstract description 12
- 230000000844 anti-bacterial effect Effects 0.000 title claims description 33
- 239000003899 bactericide agent Substances 0.000 title claims description 32
- 238000011282 treatment Methods 0.000 title claims description 13
- 239000003814 drug Substances 0.000 claims abstract description 17
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000010949 copper Substances 0.000 claims abstract description 12
- 229910052802 copper Inorganic materials 0.000 claims abstract description 12
- 239000005821 Propamocarb Substances 0.000 claims abstract description 11
- WZZLDXDUQPOXNW-UHFFFAOYSA-N propamocarb Chemical compound CCCOC(=O)NCCCN(C)C WZZLDXDUQPOXNW-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000005782 Fluopicolide Substances 0.000 claims description 11
- GBOYJIHYACSLGN-UHFFFAOYSA-N fluopicolide Chemical compound ClC1=CC(C(F)(F)F)=CN=C1CNC(=O)C1=C(Cl)C=CC=C1Cl GBOYJIHYACSLGN-UHFFFAOYSA-N 0.000 claims description 11
- 239000001963 growth medium Substances 0.000 claims description 9
- 229940079593 drug Drugs 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 2
- 239000002609 medium Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 23
- 201000010099 disease Diseases 0.000 abstract description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 8
- 235000016709 nutrition Nutrition 0.000 abstract description 4
- 230000035764 nutrition Effects 0.000 abstract description 4
- 239000002253 acid Substances 0.000 abstract description 2
- 230000000855 fungicidal effect Effects 0.000 abstract 4
- 239000000417 fungicide Substances 0.000 abstract 4
- 208000035240 Disease Resistance Diseases 0.000 abstract 1
- 239000005783 Fluopyram Substances 0.000 abstract 1
- KVDJTXBXMWJJEF-UHFFFAOYSA-N fluopyram Chemical compound ClC1=CC(C(F)(F)F)=CN=C1CCNC(=O)C1=CC=CC=C1C(F)(F)F KVDJTXBXMWJJEF-UHFFFAOYSA-N 0.000 abstract 1
- 239000003864 humus Substances 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 9
- 206010059866 Drug resistance Diseases 0.000 description 6
- 235000013339 cereals Nutrition 0.000 description 4
- 230000003385 bacteriostatic effect Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000001988 toxicity Effects 0.000 description 3
- 231100000419 toxicity Toxicity 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- 241001451172 Fusarium pseudograminearum Species 0.000 description 1
- 241000233639 Pythium Species 0.000 description 1
- 241000813090 Rhizoctonia solani Species 0.000 description 1
- 208000032023 Signs and Symptoms Diseases 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/12—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
本发明涉及农业技术领域，公开了一种三元复配杀菌剂及其在小麦茎基腐病防治中的应用，所述的三元复配杀菌剂包括氟吡菌胺、霜霉威和腐植酸铜三种有效成分，且三种成份的质量份数比为6:(6～12):(4～9)。本发明的三元复配杀菌剂在小麦茎基腐病防治中的应用，不仅能提高小麦茎基腐病的防效；同时还能增加小麦营养、提高小麦的生长势，缓解小麦茎基腐病抗药性的发生，该三元复配杀菌剂在田间表现出一定的增产效果，小麦增产量达609.65kg/667m2，增产率达18.97％；对小麦茎基腐病的防效达到80.07％，且用药最佳时间在发病初期，对小麦茎基腐病的防控具有较高的参考作用，不影响小麦产量。The invention relates to the technical field of agriculture, and discloses a ternary compound fungicide and its application in the prevention and control of wheat stem base rot. The ternary compound fungicide comprises fluopyram, propamocarb and humus There are three active components of copper acid, and the mass ratio of the three components is 6:(6-12):(4-9). The application of the ternary compound fungicide of the invention in the prevention and control of wheat stem base rot can not only improve the control effect of wheat stem base rot, but also can increase the nutrition of wheat, improve the growth potential of wheat, and relieve wheat stem base rot. The occurrence of disease resistance, the ternary compound fungicide showed a certain yield increase effect in the field, the wheat yield increased by 609.65kg/667m 2 , and the yield increase rate was 18.97%; the control effect on wheat stem rot reached 80.07% , and the best time to use the medicine is in the early stage of the disease, which has a high reference effect on the prevention and control of wheat stem rot, and does not affect the wheat yield.
Description
Technical Field
The invention relates to the technical field of agriculture, in particular to a ternary compound bactericide and application thereof in prevention and treatment of wheat stem basal rot.
Background
Wheat stem basal rot is one of the important diseases of wheat, occurs all over the world, belongs to global diseases and tends to be aggravated year by year. The disease occurs in China Hebei, Heilongjiang, Shaanxi and other areas, and the incidence rate is as high as 30.45-80.02%. At present, disease-resistant varieties can be planted, and agricultural control, biological control and chemical control can be used for controlling wheat stem basal rot. The chemical control is widely applied in production, but the long-term use of the agents can cause the wheat to generate drug resistance, which increases the difficulty for the control of the wheat stem basal rot, so that a safe and effective measure is urgently needed to deal with the wheat stem basal rot.
Disclosure of Invention
The invention aims to solve the technical problem of overcoming the defects in the prior art and provides a ternary compound bactericide, which adopts the ternary compound of fluopicolide, propamocarb and copper humate, and can improve the control effect of wheat stem base rot; meanwhile, the nutrition of the wheat can be increased, the growth vigor of the wheat can be improved, and the drug resistance of the wheat stem rot can be relieved.
In order to solve the technical problem, the invention provides a ternary compound bactericide which comprises three effective components, namely fluopicolide, propamocarb and copper humate, wherein the mass part ratio of the three effective components is 6 (6-12) to 4-9.
The application of the ternary compound bactericide in the prevention and treatment of the stem base rot of wheat is to prepare a ternary compound bactericide solution from fluopicolide, propamocarb and copper humate according to the mass part ratio of 6 (6-12) to (4-9) and spray the ternary compound bactericide solution at a seedling stage, a tillering stage and a jointing stage, and the result shows that the ternary compound bactericide shows a certain yield increase effect in the field, and the yield increase of wheat reaches 609.65kg/667m2The yield is increased by 18.97%; the control effect on the wheat stem basal rot reaches 80.07%, the optimal medication time is in the initial stage of disease occurrence, the ternary compound bactericide has a higher reference effect on the control of the wheat stem basal rot, the wheat yield is not influenced, and the ternary compound bactericide has a good development and utilization value.
The application of the drug-containing culture medium obtained by culturing the ternary compound bactericide in the prevention and treatment of wheat stem basal rot is as follows: the preparation method comprises the steps of preparing fluopicolide, propamocarb and copper humate into a ternary compound bactericide liquid medicine according to the mass part ratio, sucking 2mL of the ternary compound bactericide liquid medicine, placing the ternary compound bactericide liquid medicine into a culture dish with the diameter of 9cm, adding a dissolved PDA culture medium, and uniformly mixing to prepare a medicine-containing culture medium.
The ternary compound bactericide can improve the control effect of the wheat stem basal rot, the control effect of the wheat stem basal rot reaches 80.07 percent, and the EC is50A value of 0.81 mg/kg; meanwhile, the nutrition of the wheat can be increased, the growth vigor of the wheat is improved, the occurrence of drug resistance of the wheat stem basal rot is relieved, and a theoretical basis is provided for effectively preventing and treating the wheat stem basal rot and delaying the generation of the drug resistance.
Drawings
FIG. 1 shows the growth of hyphae in different combinations.
Detailed Description
Example 1
A ternary compound bactericide comprises 2 parts of fluopicolide, 3 parts of propamocarb and 3 parts of copper humate in parts by mass.
Example 2
A ternary compound bactericide comprises three effective components, namely fluopicolide, propamocarb and copper humate, in parts by mass of 3 parts, 3 parts and 2 parts respectively.
Example 3
A ternary compound bactericide comprises 2 parts of fluopicolide, 2 parts of propamocarb and 3 parts of copper humate in parts by mass.
Example 4
A ternary compound bactericide comprises 2 parts of fluopicolide, 4 parts of propamocarb and 2 parts of copper humate in parts by mass.
The rhizoctonia solani (Fusarium pseudograminearum) used in the above examples was provided by the plant protection laboratory of the institute of science and technology, anhui, fluopicolide, pythium, and nitrohumic acid copper, respectively, from bayer, kyushai chemical ltd, and kyushan agricultural science and technology development ltd.
1. Indoor toxicity determination:
1.1, preparing a drug-containing culture medium:
2mL of the ternary complex bactericide of examples 1 to 4 was pipetted into a petri dish with a diameter of 9cm, and then added into the dissolved PDA culture medium and mixed well to prepare four kinds of media containing the drug, namely A (2-3-3), B (3-3-2), C (2-2-3) and D (2-4-2).
1.2, determining the toxicity of the ternary compound bactericide:
placing the medicated culture medium A, B, C, D and control group (CK) into 6mm fungus cake punched by puncher, culturing in incubator (27 + -1 deg.C, humidity 65%), repeating for 3 times, measuring hypha with cross method on days 1, 3, 5, and 7 with sterilized water as control, and calculating antibacterial rate and EC50Value (concentration for 50% of maximum effect, concentration of half maximum effect, meaning the concentration that causes 50% of the maximum effect) and a toxicity regression equation.
As shown in figure 1, the growth conditions of hyphae of different combinations have better bacteriostatic effects compared with those of a control group, wherein the colony of the combination A (2-3-3) is the smallest, the bacteriostatic rate can reach 69.01%, and the bacteriostatic rates of the combination B (3-3-2), the combination C (2-2-3) and the combination D (2-4-2) are 62.43%, 58.21% and 50.16% respectively.
As can be seen from the results of the indoor toxicity test of different medicament treatments on the wheat stem basal rot (Table 1), the EC of the compound combination A (2-3-3)50A value of 0.81mg/kg, EC for the combination of B (3-3-2), C (2-2-3) and D (2-4-2)50Values were 0.76, 1.07 and 1.47mg/kg, respectively.
TABLE 1
| Combination of | Regression equation of virulence | Correlation coefficient | EC50(mg/kg) | X2 |
| A(2-3-3) | Y＝0.652X+4.062 | 0.968 | 0.81 | 1.56 |
| B(3-3-2) | Y＝0.223X+5.480 | 0.989 | 0.76 | 1.26 |
| C(2-2-3) | Y＝1.037X+3.872 | 0.898 | 1.07 | 2.18 |
| D(2-4-2) | Y＝1.223X+4.017 | 0.869 | 1.47 | 2.33 |
| CK | / | / | / | / |
2. The field control effect of the wheat stem base rot is as follows:
the Sixian county town of Sixian city of Onhui province, which has perennial wheat stem root rot, is selected as a test point, 33 degrees of 35 '22.06' in northern latitude, 118 degrees of 03 '10.87' in east longitude, the soil pH is 6-7, the fertility is uniform, and the land is smooth. The wheat variety is Nicotiana-19, the sowing is carried out in 2018 in 10 months and 8 days, and the wheat field cultivation management is the same as the conventional management.
Five treatment areas are arranged in the field and respectively used as a first test area (2-3-3), a second test area (3-3-2), a third test area (2-2-3), a fourth test area (2-4-2) and a comparison area (sprayed with sterilized water), and the area of each test area is 20m2Repeating the treatment for 3 times, arranging the treatment blocks in a random block mode, and arranging protection lines at the edge of each treatment block. The spray control of the wheat stem basal rot is carried out in three stages: seedling stage, tillering stage and jointing stage.
The disease index (%) and the control effect (%) were calculated according to the disease classification of the stem base rot of wheat (shown in table 2) by investigating 10 wheat plants per spot by a checkerboard sampling method (after spraying for 20 days).
TABLE 2
| Rank of | Symptoms and |
| Level | |
| 0 | The whole plant has no obvious symptoms |
| Level 1 | The 1 st leaf sheath of the plant is browned, and the browned area is less than 20 percent of the length of the |
| Stage | |
| 2 | The 1 st leaf sheath of the plant is browned, and the browned area accounts for about 20 to 50 percent of the length of the leaf sheath |
| Grade 3 | The 1 st leaf sheath of the plant is browned, the browned area accounts for more than 50 percent of the length of the leaf sheath, and the 2 nd leaf sheath begins to be browned |
| 4 stage | The 2 nd leaf sheath of the plant is browned, and the browned area accounts for less than 20 percent of the length of the leaf |
| Grade 5 | The 2 nd leaf sheath of the plant is obviously withered, and the browning area accounts for about 20 to 50 percent of the length of the leaf |
| Grade 6 | The 2 nd leaf sheath of the plant is totally yellow, and the 3 rd leaf tip gradually fades to green |
| Stage 7 | The 3 rd leaf of the plant is obviously browned |
The results are shown in table 3, the spraying control effects of the medicaments at different periods are different, the control effect difference of each combination is obvious, and the control effect at the seedling stage is 67.83-80.07%; the prevention effect in the tillering stage is 51.01-63.39%; the prevention effect in the jointing stage is 40.02 to 50.09 percent. The ternary compound bactericide can effectively control the wheat stem base rot.
TABLE 3
3. Wheat yield and yield constitutive factors:
and (3) adopting a diagonal sampling method in the harvest period, taking 30 wheat plants in each treatment area, repeating the steps for 3 times, investigating and recording the ear number, the ear grain number and the thousand grain weight, and obtaining the yield and the yield increase rate.
The results are shown in Table 4, and compared with the CK control group, the compound bactericide combining A (2-3-3), B (3-3-2), C (2-2-3) and D (2-4-2) has different increases in spike number, spike grain number, thousand grain weight and yield, and the yield is increased by 14.77-18.97%.
TABLE 4
The above results show that: the ternary compound bactericide disclosed by the invention can improve the control effect of the wheat stem basal rot, the control effect of the wheat stem basal rot reaches 80.07%, and meanwhile, the ternary compound bactericide can also increase the nutrition of wheat, improve the growth vigor of wheat, relieve the occurrence of drug resistance of the wheat stem basal rot and provide a theoretical basis for effectively preventing and treating the wheat stem basal rot and delaying the generation of drug resistance.
The foregoing is only the preferred embodiment of the present invention. It should be noted that, for those skilled in the art, without departing from the principle of the present invention, several modifications and equivalents may be made to the technical solution of the present invention, and the technical effects of the present invention may be achieved, and shall also be regarded as falling within the protection scope of the present invention.
Claims (4)
1. The ternary compound bactericide is characterized by comprising three effective components of fluopicolide, propamocarb and copper humate in parts by mass of 6 (6-12) and 4-9.
2. The application of the ternary complex bactericide as claimed in claim 1 in the prevention and treatment of wheat stem basal rot.
3. The application of the drug-containing culture medium obtained by culturing the ternary compound bactericide according to claim 1 in the prevention and treatment of wheat stem basal rot.
4. The use of claim 3, wherein said drug-containing medium is prepared by: the preparation method comprises the steps of preparing fluopicolide, propamocarb and copper humate into a ternary compound bactericide liquid medicine according to the mass part ratio, sucking 2mL of the ternary compound bactericide liquid medicine, placing the ternary compound bactericide liquid medicine into a culture dish with the diameter of 9cm, adding a dissolved PDA culture medium, and uniformly mixing to prepare a medicine-containing culture medium.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1494377A (en) * | 2001-03-08 | 2004-05-05 | �ݶ�ũ�ƹɷ�����˾ | Fungicide compositions based on pyridylethylbenzamide and propamocarb derivative |
| CN102919256A (en) * | 2012-11-28 | 2013-02-13 | 河北省农林科学院植物保护研究所 | Fluopicolide-containing compound bactericide and application thereof |
| CN103004819A (en) * | 2012-11-30 | 2013-04-03 | 王学权 | Fluopicolide-containing bactericide composition |
| CN106664887A (en) * | 2017-01-19 | 2017-05-17 | 恩施土家族苗族自治州农业科学院 | Selenium-enriched potato planting method capable of preventing late blight by using seed potatoes with selenium-enriched coatings |
-
2021
- 2021-02-24 CN CN202110209528.XA patent/CN112970760A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1494377A (en) * | 2001-03-08 | 2004-05-05 | �ݶ�ũ�ƹɷ�����˾ | Fungicide compositions based on pyridylethylbenzamide and propamocarb derivative |
| CN102919256A (en) * | 2012-11-28 | 2013-02-13 | 河北省农林科学院植物保护研究所 | Fluopicolide-containing compound bactericide and application thereof |
| CN103004819A (en) * | 2012-11-30 | 2013-04-03 | 王学权 | Fluopicolide-containing bactericide composition |
| CN106664887A (en) * | 2017-01-19 | 2017-05-17 | 恩施土家族苗族自治州农业科学院 | Selenium-enriched potato planting method capable of preventing late blight by using seed potatoes with selenium-enriched coatings |
Non-Patent Citations (3)
| Title |
|---|
| 商鸿生等: "《玉米病虫害防治方法》", 30 August 2007 * |
| 王巍等: "《北方农药新品种实用手册》", 30 April 2019 * |
| 马奇祥等: "《常用农药使用简明手册》", 31 January 2001 * |
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