CN104585236B - KN 2 and application of the complex preparation in tobacco mosaic virus (TMV) is prevented containing KN 2 - Google Patents

Abstract

The invention relates to the technical field of pesticides, and discloses the application of KN-2 and a compound preparation containing KN-2 in preventing and treating tobacco mosaic virus. The invention discloses that KN-2 can induce TMV to produce specific disease resistance, and effectively prevent and treat tobacco mosaic virus disease. The compound preparation SKGM-7 containing KN-2 can effectively prevent and control the harm and loss of tobacco virus disease. The use of KN‑2 or SKGM‑7 compound formulation can not only effectively prevent and treat tobacco virus disease (TMV), but also greatly improve the yield and quality of tobacco leaves, resulting in considerable economic benefits.

Description

Application of KN-2 and the compound preparation containing KN-2 in the prevention and treatment of tobacco mosaic virus

technical field

The invention relates to the technical field of pesticides, in particular to the application of KN-2 and a compound preparation containing KN-2 in preventing and treating tobacco mosaic virus.

Background technique

There are many types of tobacco virus diseases, and the transmission route is also relatively complicated. At present, tobacco mosaic virus (TMV) is the main disease in my country's tobacco, and the incidence rate is about 70% of the whole virus diseases. Tobacco virus The host range of TMV is very wide. Nearly 10 families and more than 100 kinds of plants can be infected with TMV to cause disease. Among them, Solanaceae, Brassicaceae, and Compositae are the most affected. These crops and plants often cause disease after being infected. serious loss. Tobacco common mosaic virus disease is usually transmitted by contact during various agricultural operations, especially in the seedling shed where the seedlings are concentrated. After the tobacco seedlings and tobacco plants are infected with the virus disease, the leaves appear dark green or light green. Embedded mottled, so called mosaic disease, tobacco plants in the early stage of disease, leaves mosaic, plants dwarf, with the development of the disease, leaves twisted not long, appear various necrotic spots, tobacco plants are only about half of normal tobacco plants , Tobacco leaves basically lose their utilization value. Tobacco virus diseases are therefore the most important diseases of tobacco production and other susceptible crops.

At present, there are basically three categories of pesticides commonly used to prevent and treat plant virus diseases. The first class of drugs mainly inhibits the synthesis and replication of viral nucleic acids and inhibits virus proliferation. This class of drugs includes morpholine hydrochloride, Ningnanmycin, etc. The main mechanism of action of drugs is to promote chlorophyll synthesis, promote cell growth, promote cell division, and promote root growth. Such drugs include: brassinolide, indole acetic acid, gibberellic acid, etc.; To produce broad-spectrum disease resistance, such agents include amino oligosaccharins, mixed fatty acids, lentinan and so on. These drugs have a certain effect on viral diseases, but because the targets of inducing pathogens are too wide, they lack specificity and specialization, and the effect of preventing and treating viral diseases is also limited. The control effect of these medicines on tobacco virus disease is generally below 50%. The above-mentioned medicines are crossed and used in combination, and the control effect will not exceed 60%. According to statistics, at present, the loss of tobacco and other crops caused by tobacco virus disease (TMV) in my country is as high as more than 30 billion yuan. At present, there is an urgent need to find better medicaments for preventing and treating tobacco virus disease in order to control and restore the tobacco common disease. Economic loss of crops due to mosaic virus disease (TMV).

KN-2 is a foliar fertilizer containing 3.6% active sodium silicomomolybdate. The ability of crops to resist frost, drought and pests and diseases. The dilution factor when spraying is required to be 4000-5000 times.

Contents of the invention

The invention provides a new application of KN-2 in preventing and treating tobacco mosaic virus disease. At the same time, it provides a compound preparation containing KN-2 and its application in preventing and treating tobacco mosaic virus disease.

In recent years, we have done a lot of work on the screening of tobacco virus disease prevention and control agents. On the basis of careful analysis, summary and collection of domestic and foreign plant virus prevention and control agents, and inspired by the successful experience of human "cocktail therapy" against HIV , we screened the formulations of existing viral disease prevention and control agents. The main drugs, active ingredients and concentrations used in each test are shown in Table 1.

Table 1: Types of medicaments screened for tobacco virus disease control formulations

Table 2: Types and manufacturers of formulations for the prevention and treatment of tobacco virus disease

Note: Nos. 4, 7, and 8 do not have registration certificates because they are foliar fertilizers.

These agents include virus inhibitors, plant growth regulators, foliar fertilizers, organic silicon synergists, etc., as well as KN-2 produced by Belgium Runkang Company. We diluted KN-2 2500 times and sprayed it on the tobacco leaf plants infected with TMV, and sprayed it every ten days. The relative control effect was 71.9% 20 days after the last spraying, which was higher than that of the control drug Kangkai-1 ( 42.07%), 14% Mansuke Zinc (39.23%), and 8% Ningnanmycin (43.36%). It is proved that KN-2 can be used in the prevention and treatment of tobacco mosaic virus disease, and its use at 2500 times has a good control effect on tobacco mosaic virus disease. KN-2 can induce TMV to produce specific disease resistance, but other mechanisms are unknown. Therefore, the invention protects the application in the prevention and treatment of tobacco mosaic virus disease.

The invention also protects the application of the compound preparation of KN-2 in preventing and treating tobacco mosaic virus disease.

Preferably, the main component of the compound preparation is KN-2, and may also include a mixture of two or more of plant virus inhibitors, plant growth regulators, foliar fertilizers or organic silicon synergists.

More preferably, the plant virus inhibitor is Virus Guangguang, Jiatai, Junkeduke or mixed fat·copper sulfate, and the plant growth regulator or foliar fertilizer is Kangkai-1, Mansuke, Amino Oligosaccharins, lentinan or hypersensitive protein particles.

The present invention dilutes single agents such as virus inhibitors, plant growth regulators, and foliar fertilizers with KN-2 and then mixes them, and then screens the compound preparations of tobacco mosaic virus disease, and screens out the compounds that can effectively prevent and treat tobacco mosaic leaves. Viral antiviral formula preparation SKGM-7. SKGM-7 is prepared by mixing 2500 times of KN-2 dilution, 600 times of virus light and light dilution, and 3000 times of 14% Mansuko zinc dilution in equal amounts of 1:1:1. The mass ratio of KN-2, virus light, and 14% triclosan in the antiviral compound preparation SKGM-7 was 6:25:5. Among them, KN-2 induces TMV to produce specific disease resistance; virus light prevents viral DNA transcription and protein synthesis, and inhibits the synthesis of viral nucleic acid polymerase; Mansuke zinc can promote rooting and enhance plant disease resistance. The mixture of the three can effectively prevent and treat the harm of tobacco virus disease.

Tobacco virus disease prevention and treatment agent KN-2 of the present invention and compound preparation SKGM-7 can effectively prevent and treat the harm and the loss of tobacco virus disease, use KN-2 or SKGM-7 compound preparation, not only can effectively prevent and treat tobacco virus disease (TMV ), and the yield and quality of tobacco leaves are greatly improved. The ratio of input to output is 1:23.0 by using the compound preparation of the present invention to prevent and treat tobacco virus disease, that is, the cost of medicine for three times of medication is 90 yuan/mu, and the economic income of tobacco leaves can be increased by 2077 yuan. If the whole country uses medicament of the present invention by 5,000,000 mu of tobacco (national tobacco planting area is 45,000,000 mu), then the economic income of tobacco farmers can increase by 7.55 billion yuan. If the scope of application is further expanded, the medicament formula of the present invention can be used on other crops infected with tobacco virus disease (TMV) to prevent and control virus diseases, and can also produce greater economic benefits.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

The dilution mentioned in the present invention, unless otherwise specified, all refers to dilution with water, and the dilution times are volume multiples.

Example 1:

The field control experiment was carried out in the smoke field of Longtan Sishe, Puwei Township, Puwei Township, Miyi County, Panzhihua City. The experimental area is located in the flue-cured tobacco planting area in the deep hills of southwest Sichuan. The altitude is 1800 meters, the terrain of the test site is flat, the soil texture is yellow loam, the pH value is 5.7, weakly acidic, and the organic matter content is 3.0%. The previous work is leisure.

Transplanted and planted on May 6, the planting density was 1100 plants/mu, and the tobacco was growing well. The cultivation conditions of the experimental plots were basically the same.

Virus inoculation of tobacco strains in the field: Virus inoculation was carried out on all tobacco strains tested 3 days before spraying. Tobacco mosaic (TMV) poisonous leaves identified indoors were ground into juice with corundum, and the leaves of tobacco plants were frictionally inoculated with sterilized cotton swabs dipped in the poisonous juice.

Design dosage according to Table 2, according to plot area, accurately measure dosage and be made into medicinal liquid, according to mu medicinal liquid amount 45L, utilize HD-400 manual sprayer (pressure 45Pa, injection rate 710ml/min) to carry out stem and leaf spray. The use concentration of the antiviral compound preparation SKGM-7 is that each single dose is prepared according to the dilution factor in Table 1, and then mixed uniformly in equal amounts of 1:1:1 to form the compound preparation SKGM-7.

Table 3: Types and concentrations of compound preparations for field tobacco virus disease control

Table 4: Active ingredients and mechanism of action

Time and frequency of spraying: Spray for the first time after transplanting in the field, and once every ten days, for a total of three times. Spray once on May 15th, May 25th and June 4th.

Investigation time and frequency: 10 days and 20 days after the last spraying, the incidence level was investigated. The evaluation of control effect shall be based on the second time.

investigation method:

Investigate all the tobacco plants in each plot, record the incidence according to the following grading method, and calculate the disease index and control effect. During spraying, observe whether the test medicament affects the normal growth and growth situation of the tobacco plants.

Tobacco Virus Disease Severity Grading Standard (National Standard GB/T23222-2008) Grading survey in units of strains:

Grade 0: The whole plant is disease-free;

Grade 1: Heart leaves with bright veins or slightly mosaic leaves, no obvious dwarfing of diseased plants;

Grade 3: One-third of the leaves are mosaic but not deformed, or the diseased plants are dwarfed to more than three-quarters of the normal plant height;

Grade 5: One-third to one-half of the leaves are mosaic, or a few leaves are deformed, or the main veins are black, or the diseased plants are dwarfed to two-thirds to three-quarters of the normal plant height;

Grade 7: One-half to two-thirds of the leaves are mosaic, or deformed or the main lateral veins are necrotic, or the diseased plants are dwarfed to one-half to two-thirds of the normal plant height;

Grade 9: The leaves and mosaic of the whole plant are severely deformed or necrotic, or the diseased plant is dwarfed to more than half of the normal plant height.

Drug efficacy calculation method

Survey of agronomic traits and yield value

The investigation of agronomic traits is mainly to measure the plant height and the number of effective leaves of all tobacco plants in each plot and treatment before the topping of the tobacco plants, and calculate the average value of each treatment. The determination of yield and output value needs to harvest the tobacco plants of each treatment in each plot, tie them up and bake them separately, weigh them separately, and price them in grades, and then calculate and convert the yield per mu and yield value per mu of each treatment, and compare the obtained data with the blank Calculate the percentage increase of output and output value of each treatment.

2. Test results

The various survey data and effects of preventing and treating tobacco mosaic disease (TMV) in the field are shown in Table 4, and the disease index of each treatment was investigated 20 days after application. The disease index of the two treatments of agent KN-2 and the three control agents were 15.29, 18.79, 38.72, 40.63 and 37.86 respectively, and the disease index of the blank control area was 66.86. Based on this, the control effects of the two treatments of the antiviral compound preparation were calculated to be 77.13% and 71.90%, respectively. The control effect of the control drug Kangkai-1 was 42.07%, the control effect of the control drug 14% Mansuke zinc was 39.23%; the control effect of the control drug 8% Ningnanmycin was 43.36%. The results showed that the disease prevention effects of the two treatments of the antiviral compound preparation SKGM-7 and KN2 both reached more than 70%, which was about 30% higher than that of several control agents, and the relative control effect was increased by 59.61%.

Table 5: Survey on the effectiveness of various antiviral agents and compound preparations in the prevention and treatment of tobacco virus disease (TMV)

3. Survey of agronomic traits and economic benefits

The average plant height and average leaf number surveyed 30 days after the last spraying also showed that the SKGM-7 formula increased the plant height by 41.33% and the leaf number by 31.35% compared with the blank control. Each mu of tobacco leaves can increase the output by more than 30%, and the output value can be increased by more than 50%. The test results show that the two formulations of the antiviral compound SKGM-7 and the plant virus-inducing resistance agent KN-2 are very effective, and can completely control the occurrence and spread of tobacco virus diseases in the field. At the same time, our formula has also fully considered the cost of prevention and control and the practical problems of large-scale promotion and application. The ratio of input to output is 1:23.0, that is, the cost of chemicals is 10 yuan/mu, and the economic income of tobacco leaves can be increased by 230 yuan.

Table 6: Survey form of agronomic traits, yield and output value of each treatment tobacco

4. Demonstration and promotion

The compound preparation SKGM-7 and KN-2 of the prevention and treatment of tobacco virus disease of the present invention are through two years of field tests and demonstration promotions in each main tobacco area of Panzhihua City and Sichuan Province. The demonstration promotion area is about 20,000 mu, all of which prove this The invented compound preparations SKGM-7 and KN-2 for the prevention and treatment of tobacco virus disease have remarkable effect on the prevention and treatment of tobacco virus disease (TMV), and have achieved good economic and social benefits. It is proved that the compound preparations SKGM-7 and KN-2 for preventing and treating tobacco virus diseases of the present invention have stable control effects.

The above embodiments are intended to illustrate that the present invention can be implemented or used by those skilled in the art. It will be obvious to those skilled in the art to modify the above embodiments, so the present invention includes but is not limited to the above embodiments. Any method, process, or product that conforms to the claims or the description of the specification, and conforms to the principles, novelty, and creative features disclosed herein falls within the protection scope of the present invention.

Claims (9)

1. Applications of the 1.KN-2 in tobacco mosaic virus disease is prevented.
2. 2. application according to claim 1, it is characterised in that KN-2 is diluted to 2500 times of liquid and is imposed on crop.
3. 3. a kind of complex preparation containing KN-2, it is characterised in that the active ingredient of the complex preparation is KN-2, and plant One or both of viral inhibitors, plant growth regulator, foliar fertilizer or organosilicon synergist any of the above ratio are mixed Close.
4. 4. the complex preparation according to claim 3 containing KN-2, it is characterised in that the active ingredient of the complex preparation Can zinc for KN-2, viral light light and 14% full element；

   The virus light light is 30% morpholine biguanidine hydrochloride and 1% ribavirin wettable powder.
5. 5. the complex preparation of KN-2 according to claim 4, it is characterised in that the KN-2, viral light light, 14% full element Can zinc mass ratio be 6：25：5.
6. 6. such as application of the claim 3-5 any one of them complex preparation in tobacco mosaic virus disease is prevented.
7. 7. application according to claim 6, it is characterised in that the main component of the complex preparation is KN-2, Yi Jizhi Two or more mixing in thing viral inhibitors, plant growth regulator, foliar fertilizer or organosilicon synergist.
8. 8. application according to claim 7, it is characterised in that the plant virus inhibitor is viral light light, sows safe, bacterium Gram poison gram or mixed fat copper sulphate, the plant growth regulator or foliar fertilizer are Kang Kai -1, full element can zinc, amino-oligosaccharide, Lentinan or super quick protein particle；

   The virus light light is 30% morpholine biguanidine hydrochloride and 1% ribavirin wettable powder.
9. 9. application according to claim 8, it is characterised in that KN-2 is diluted to 2500 times of liquid, viral light light is diluted to 600 times of liquid, 14% full element can zinc be diluted to 3000 times of liquid, three presses 1:1:1 volume ratio is imposed on crop after mixing.