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CN110839558B - A behavioral experimental device and experimental method for animal auditory association matching - Google Patents

A behavioral experimental device and experimental method for animal auditory association matching Download PDF

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Publication number
CN110839558B
CN110839558B CN201911133665.9A CN201911133665A CN110839558B CN 110839558 B CN110839558 B CN 110839558B CN 201911133665 A CN201911133665 A CN 201911133665A CN 110839558 B CN110839558 B CN 110839558B
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CN110839558A (en
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鲍宇
孟志强
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Shenzhen Institute of Advanced Technology of CAS
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Shenzhen Institute of Advanced Technology of CAS
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Priority to PCT/CN2020/129169 priority patent/WO2021098655A1/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K29/00Other apparatus for animal husbandry
    • A01K29/005Monitoring or measuring activity, e.g. detecting heat or mating
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K15/00Devices for taming animals, e.g. nose-rings or hobbles; Devices for overturning animals in general; Training or exercising equipment; Covering boxes
    • A01K15/02Training or exercising equipment, e.g. mazes or labyrinths for animals ; Electric shock devices ; Toys specially adapted for animals
    • A01K15/027Exercising equipment, e.g. tread mills, carousels

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  • Environmental Sciences (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
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  • General Health & Medical Sciences (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
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Abstract

本发明公开了一种动物听觉联想匹配的行为学实验装置及实验方法,该行为学实验装置包括壳体、遮光板、检测组件、翻板、发声组件和控制组件。壳体内设有多个隔板,多个隔板将壳体内的空间分隔出多个腔体,每个腔体内均设有分割板,分割板将腔体分隔为实验腔体和食物腔体,壳体上设有多个伸手口,每个伸手口连通一个实验腔体,分割板上设有取食口以连通实验腔体和食物腔体,遮光板可滑动地设在壳体上,遮光板用于遮挡设有多个伸手口的壳体的侧壁,翻板为多个,每个翻板对应一个取食口设置,发声组件用于朝向实验动物发出声音刺激,该行为学实验装置能够较好地量化分析动物听觉与行为之间的联想匹配关系,从而更为准确地分析脑区颞叶的功能。

Figure 201911133665

The invention discloses a behavioral experimental device and an experimental method for animal auditory association matching. The behavioral experimental device includes a casing, a shading plate, a detection component, a flap, a sounding component and a control component. A plurality of partitions are arranged in the casing, and the plurality of partitions divide the space in the casing into a plurality of cavities. Each cavity is provided with a dividing plate, and the dividing plate divides the cavity into an experimental cavity and a food cavity. The shell is provided with a plurality of hand openings, each of which is connected to an experimental cavity, the dividing plate is provided with a feeding port to connect the experimental cavity and the food cavity, and the shading plate is slidably arranged on the shell to block light. The plate is used to cover the side wall of the casing with a plurality of hand openings, there are multiple flaps, each flap is set corresponding to a feeding port, and the sound-emitting component is used to emit sound stimulation toward the experimental animals. The behavioral experimental device It can better quantitatively analyze the associative matching relationship between animal hearing and behavior, so as to analyze the function of the temporal lobe of the brain more accurately.

Figure 201911133665

Description

Animal auditory association matching behavioristics experimental device and experimental method
Technical Field
The invention relates to the technical field of animal ethology research, in particular to an animal auditory sense association matching ethology experimental device and an animal auditory sense association matching ethology experimental method.
Background
With the development of scientific technology, animals have been widely used in medical biology (including behavioural) testing and research due to great resemblance in neuroanatomy, neurophysiology, immunology and genetic characteristics to humans. Behavioral training is the study of clinical characteristics of animals, and animals complete target tasks in training to achieve the purpose of evaluating certain indexes so as to reveal the essence and the law of life activities, health and diseases. However, it is difficult and complicated to deploy a reasonable behavioral apparatus because it is a large, smart, social, long-lived, non-domesticated, and offensive animal. The previous research on cognitive memory of each visual space focuses on the test of working memory.
At present, the prior art does not provide a device capable of accurately quantifying the matching of the animal auditory sense and the behavior, most of the prior devices can only carry out qualitative analysis, the analysis result is not accurate, the relation between the auditory sense and the behavior of the animal can not be well reflected, and therefore the function of the temporal lobe of the brain area corresponding to the animal can not be well analyzed.
Disclosure of Invention
The invention aims to provide a behavioral experiment device for animal auditory sense association matching, which can better quantitatively analyze association matching relation between animal auditory sense and behaviors so as to more accurately analyze functions of temporal lobes of brain areas.
The invention aims to provide a behavioral experiment method for animal auditory sense association matching, which can better quantitatively analyze association matching relation between animal auditory sense and behavior.
In order to achieve the technical effects, the technical scheme of the invention is as follows:
a behavioral experiment device for auditory association matching of animals, comprising: the device comprises a shell, a plurality of partition plates are arranged in the shell, the plurality of partition plates divide the space in the shell into a plurality of cavities, each cavity is internally provided with a partition plate, the partition plates divide the cavity into an experiment cavity and a food cavity, the shell is provided with a plurality of hand extending ports, each hand extending port is communicated with one experiment cavity, and the partition plates are provided with food taking ports so as to be communicated with the experiment cavity and the food cavity; the shading plate is arranged on the shell in a sliding mode and used for shading the side wall of the shell provided with the plurality of hand stretching openings; a detection component configured to detect whether the light shielding plate closes the hand stretching opening or not and detect whether an arm of the experimental animal passes through the hand stretching opening or not; the food taking device comprises a plurality of turning plates, wherein each turning plate corresponds to one food taking port; the sound production assembly is used for producing sound stimulation towards the experimental animal; the control assembly is electrically connected with the sound production assembly and the detection assembly, and the control assembly is configured to record a first time length when the light shielding plate is detached and the arm of the experimental animal stretches into the hand stretching opening and a second time length when the arm of the experimental animal stretches into the hand stretching opening and the arm of the experimental animal retreats from the hand stretching opening.
In some embodiments, the detection component comprises: the first detection piece is arranged on the side wall of the shell, which is matched with the shading plate, and is configured to detect whether the shading plate seals the hand stretching opening or not; the second detection piece, the second detection piece is a plurality of, every the second detection piece is established on one stretch out the inner wall of hand mouth, the second detection piece is configured to detect whether the arm of laboratory animal passes stretch out the hand mouth.
In some specific embodiments, the control assembly comprises: the first timer records that the first time length from the time when the first detection piece detects that the light shielding plate is separated from the side wall of the shell to the time when the second detection piece detects the arm of the experimental animal for the first time is the first time length; and the second timer records that the time from the first time that the second detection piece detects the arm of the experimental animal to the second time that the second detection piece detects the arm of the experimental animal is the second time.
In some embodiments, the control assembly includes a zero button electrically connected to the first timer, the second timer, and the second detector.
In some embodiments, the detection assembly further comprises: the third detection piece, the third detection piece is a plurality of, every the third detection piece corresponds one get food mouthful and sets up, the third detection piece is used for detecting whether experimental animals' arm passes get food mouthful.
In some specific embodiments, the detection assembly further includes a plurality of indicator lights, the plurality of indicator lights are disposed in one-to-one correspondence with the plurality of third detection members, and each indicator light is electrically connected to the third detection member.
In some embodiments, two sliding grooves are formed in the side wall of the housing, where the hand-stretching opening is formed, and two opposite side edges of the light shielding plate are fitted in the two sliding grooves.
In some embodiments, the number of cavities is at least three.
An animal auditory association matching behavioral experiment method is carried out by adopting the animal auditory association matching behavioral experiment device in the previous item, and comprises the following steps; s1: the sounding component emits prompt sound; s2: removing the light shielding plate fitted on the side wall of the housing; s3: recording a first time length from the removal of the light screen until the arm of the experimental animal extends into the hand extending opening; s4: recording a second time length from the time when the arm of the experimental animal passes through the hand stretching opening to the time when the arm of the experimental animal exits the hand stretching opening.
In some embodiments, in step S3, after the light shielding plate is removed for a first preset time, the arm of the experimental animal still does not pass through the hand stretching opening, and the experiment is stopped; in step S4, the arm of the experimental animal passes through the hand stretching opening within the second preset time, and after the arm of the experimental animal passes through the hand stretching opening for the second preset time, the arm of the experimental animal does not pass through the hand stretching opening again, and the experiment is stopped.
According to the animal auditory sense association matching ethology experiment device provided by the embodiment of the invention, the control component can record the first time length from the removal of the light screen to the arm extending-in mouth of the experimental animal and the second time length from the arm extending-in mouth of the experimental animal to the arm exiting-out mouth of the experimental animal, and the ethology experiment of the animal auditory sense association matching is divided into two time periods, so that the behaviors of the experimental animal are divided and quantitatively judged in a standardized way, and an experimenter can more accurately analyze the functions of the temporal lobe of the brain area.
According to the animal auditory sense association matching behavioristics experiment method, the animal auditory sense association matching behavioristics experiment is divided into the first time period from the removal of the shading plate to the arm extending opening of the experimental animal and the second time period from the arm extending opening of the experimental animal to the arm exiting opening of the experimental animal, so that the aim of performing standardized segmentation and quantitative judgment on the behavior of the experimental animal is fulfilled, the association matching relation between the auditory sense and the behavior of the animal can be better quantitatively analyzed, and the function of the temporal lobe of the brain area can be more accurately analyzed.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
Fig. 1 is a schematic structural diagram of a behavioral experimental apparatus for matching auditory association of animals according to an embodiment of the present invention.
Fig. 2 is a step diagram of a behavioral experimental method for auditory association matching of animals according to an embodiment of the present invention.
Reference numerals:
1. a housing; 11. stretching the hand mouth; 12. an experiment cavity; 13. a food cavity; 2. a visor; 3. a detection component; 31. a first detecting member; 32. a second detecting member; 33. a third detecting member; 4. turning over a plate; 5. a partition plate; 6. a partition plate; 61. a food taking port; 7. a zero button.
Detailed Description
In order to make the technical problems solved, the technical solutions adopted and the technical effects achieved by the present invention clearer, the technical solutions of the present invention are further described below by way of specific embodiments with reference to the accompanying drawings.
In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the invention.
Furthermore, features defined as "second" and "third" may explicitly or implicitly include one or more of the features for distinguishing between descriptive features, non-sequential, non-trivial and non-trivial. In the description of the present invention, "a plurality" means two or more unless otherwise specified.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The following describes a specific structure of an animal auditory sense association matching behavioristics experimental apparatus according to an embodiment of the present invention with reference to fig. 1.
As shown in fig. 1, the behavioral experiment apparatus for matching auditory sense association of animals according to the embodiment of the present invention includes a housing 1, a light shielding plate 2, a detection component 3, a flap 4, a sound production component (not shown), and a control component (not shown). A plurality of clapboards 5 are arranged in the shell 1, the plurality of clapboards 5 divide the space in the shell 1 into a plurality of cavities, each cavity is internally provided with a partition board 6, the cavity is divided into an experiment cavity 12 and a food cavity 13 by the partition boards 6, the shell 1 is provided with a plurality of hand stretching ports 11, each hand stretching port 11 is communicated with one experiment cavity 12, the partition boards 6 are provided with food taking ports 61 to be communicated with the experiment cavity 12 and the food cavity 13, the light shielding boards 2 are slidably arranged on the shell 1, the light shielding boards 2 are used for shielding the side wall of the shell 1 provided with the plurality of hand stretching ports 11, the detection component 3 is configured to detect whether the light shielding boards 2 seal the hand stretching ports 11 or not, detect whether the arms of the experimental animals pass through the hand stretching ports 11 or not, the turning boards 4 are a plurality of turning boards, each turning board 4 is arranged corresponding to one food taking port 61, the sounding component is used for sending, the control assembly is configured to record a first time length from the removal of the light shielding plate 2 to the arm-in hand-stretching opening 11 of the experimental animal and record a second time length from the arm-in hand-stretching opening 11 of the experimental animal to the arm-out hand-stretching opening 11 of the experimental animal.
The behavioral experimental apparatus for animal auditory association matching according to the present invention mainly performs an auditory association matching experiment on an animal. Specifically, the experiment cavities 12 are numbered in sequence before the experiment, the sounding component emits a prompt tone in the experiment process, the times of the prompt tone can stimulate the experimental animal to enable the experimental animal to generate an association matching behavior, the experimental animal can accurately take out food from the food cavity 13 corresponding to the experiment cavity 12 with the same number as the times of the prompt tone, the experimental animal is calibrated to be successfully executed, if the experimental animal stretches an arm into the wrong experiment cavity 12, the animal is calibrated to be failed to be executed, and if the sounding component emits the prompt tone for a period of time, the experimental animal does not take food, and the non-execution behavior of the experimental animal is calibrated. Therefore, the auditory association matching capability of the experimental animal can be preliminarily and qualitatively analyzed according to the specific behavior of the experimental animal. For the accuracy of the experiment, only the food cavity 13 corresponding to the correct experimental cavity 12 is filled with food, that is, only after the animal is successfully executed in the experimental process, the animal can obtain the reward, so that the auditory association matching capability of the experimental animal can be relatively accurately and qualitatively analyzed.
It will be appreciated that the flap 4 is closed to close the access port 61 before the experiment is started and the light screen 2 is mounted to the side wall of the housing 1 provided with the snout 11 to ensure that the experimental animal cannot see the inside of the experimental chamber 12 from the side wall provided with the snout 11.
After the experiment begins, place the laboratory animal in the place ahead that corresponds the lateral wall that is equipped with casing 1 of stretching hand mouth 11, sound generating mechanism sends the prompt tone, waits for the sound production to remove light screen 2 after finishing, waits for the laboratory animal to stretch into the arm and stretch into in the hand mouth 11 and control assembly record demolish light screen 2 to the first time of the arm of laboratory animal passing stretching hand mouth 11. In practical situations, the length of time from the observation of hearing the warning sound to the stretching of the arm into the hand stretching opening 11 of the experimental animal is greatly different according to the different types of the experimental animals, the different domestication degrees, the different characters of the experimental animals and the different health states. In this embodiment, the control assembly can note first duration, removes the length of time that the arm of light screen 2 to the laboratory animal stretches into and stretches into hand mouth 11 promptly, can preliminary analysis go out laboratory animal's reaction ability and the ability that the auditory association matches according to the length of first duration. In addition, the control assembly can also record the second time length from the time when the arm of the experimental animal stretches into the hand stretching opening 11 to the time when the arm of the experimental animal retreats from the hand stretching opening 11, and the second time length can further measure the execution capacity of the experimental animal.
According to the animal auditory association matching ethology experiment device, the control component can record the first time length from the removal of the light screen 2 to the stretching of the arm of the experimental animal into the hand stretching port 11 and the second time length from the stretching of the arm of the experimental animal into the hand stretching port 11 to the exiting of the arm of the experimental animal out of the hand stretching port 11, and the animal auditory association matching ethology experiment is divided into two time periods, so that the standardized division and quantitative judgment of the behavior of the experimental animal are realized, and an experimenter can more accurately analyze the function of the temporal lobe of the brain area.
In some embodiments, as shown in fig. 1, the detecting assembly 3 includes a first detecting member 31 and a second detecting member 32, the first detecting member 31 is disposed on a side wall of the housing 1 where the light shielding plate 2 is fitted, the first detecting member 31 is configured to detect whether the light shielding plate 2 closes the hand-stretching opening 11, the number of the second detecting members 32 is plural, each second detecting member 32 is disposed on an inner wall of one hand-stretching opening 11, and the second detecting member 32 is configured to detect whether an arm of the laboratory animal passes through the hand-stretching opening 11. It can be understood that the time point of the removal of the light shielding plate 2 and the time point of the arm of the experimental animal extending into the hand opening 11 can be accurately determined by arranging the first detecting part 31 and the second detecting part 32, so that the first time length recorded by the control assembly is more accurate, and the experimenter can quantitatively determine the auditory association matching level of the experimental animal more accurately.
In some embodiments, the first detecting member 31 is a light sensor, and the second detecting member 32 is a touch sensor. Therefore, the functions of detecting whether the light shielding plate 2 seals the hand stretching opening 11 and detecting whether the arm of the experimental animal passes through the hand stretching opening 11 can be conveniently realized. Of course, in other embodiments of the present invention, the specific types of the first detecting element 31 and the second detecting element 32 can be selected according to actual needs, and are not limited to the structures of the light sensor and the touch sensor of the present embodiment.
In some specific embodiments, the control assembly includes a first timer and a second timer, the first timer records a first time period from the first detecting member 31 detecting that the light shielding plate 2 is separated from the side wall of the housing 1 to the second detecting member 32 detecting the arm of the laboratory animal for the first time, and the second timer records a second time period from the second detecting member 32 detecting the arm of the laboratory animal for the first time to the second detecting member 32 detecting the arm of the laboratory animal for the second time. It can be understood that the first time length and the second time length can be accurately recorded through the first timer and the second timer, so that the experimental data can be conveniently recorded by an experimenter, and the auditory association matching phenomenon capability and the executive capability level of the experimental animal can be conveniently judged. Here, the first timer may be a physical timing device electrically connected to the first detecting element 31 and the second detecting element 32, or may be a logic program for timing of control logic of the control assembly, and the second timer may be a physical timing device electrically connected to the two third detecting elements 33, or may be a logic program for timing of control logic of the control assembly, and may be specifically selected according to actual needs.
In some embodiments, as shown in fig. 1, the control assembly includes a zero button 7, the zero button 7 being electrically connected to the first timer, the second timer, and the second detection member 32. It is understood that in the actual experiment process, the experiment may be performed on the same experimental animal for a plurality of times, or on a plurality of different experimental animals. The zero setting button 7 can realize zero setting of the first timer, the second timer and the second detection piece 32 after the completion of one experiment, and is convenient for the next experiment. It should be noted that, in this embodiment, the zero button 7 may be an entity button electrically connected to the first timer, the second timer and the second detecting element 32, or may be a logic program used by the control component to zero, and may be specifically selected according to actual needs.
In some embodiments, as shown in fig. 1, the detecting assembly 3 further includes a plurality of third detecting members 33, each third detecting member 33 is disposed corresponding to one feeding port 61, and the third detecting members 33 are used for detecting whether the arm of the experimental animal passes through the feeding ports 61. It can be understood that, the third detecting part 33 is arranged at the food taking opening 61, so that the experimenter can conveniently judge the execution result of the experimental animal, and after the third detecting part 33 is electrically connected with the control component, the control component can automatically record the execution result of the experimental animal according to the result of the third detecting part 33, so that the experimenter is not required to manually record.
In some specific embodiments, the detecting assembly 3 further includes a plurality of indicator lights, the plurality of indicator lights are disposed in one-to-one correspondence with the plurality of third detecting members 33, and each indicator light is electrically connected to the third detecting member 33. From this, the pilot lamp can send the flash of light after the arm of laboratory animal passes to get edible mouthful 61 to further made things convenient for the experimenter to observe laboratory animal's execution result, made things convenient for the experimenter to carry out the experiment record.
In some embodiments, the side wall of the housing 1 provided with the hand-stretching opening 11 is provided with two sliding grooves arranged at intervals, and two opposite side edges of the light shielding plate 2 are fitted in the two sliding grooves. Therefore, the shading plate 2 is convenient for operators to mount or dismount. Of course, in other embodiments of the present invention, the light shielding plate 2 may also be connected to the housing 1 by a screw, a rivet, or other connecting members.
In some embodiments, the number of cavities is at least three. Of course, in the embodiment of the present invention, the number of the cavities may be selected according to actual needs, and is not limited to three in this embodiment.
Example (b):
the following describes a specific structure of an experimental apparatus for animal auditory sense association matching behaviourology according to a specific embodiment of the present invention with reference to fig. 1.
As shown in fig. 1, the behavioral experiment apparatus for matching animal auditory sense association according to the embodiment of the present invention includes a housing 1, a light shielding plate 2, a detection component 3, a turning plate 4, a sound production component and a control component. Be equipped with two baffles 5 in the casing 1, three cavity is separated with the space in the casing 1 to two baffles 5, all be equipped with partition plate 6 in every cavity, partition plate 6 separates the cavity for experiment cavity 12 and food cavity 13, be equipped with three hand mouth 11 of stretching on the casing 1, every experiment cavity 12 of 11 intercommunications of hand mouth, be equipped with on the partition plate 6 and get food mouth 61 with intercommunication experiment cavity 12 and food cavity 13, establish on casing 1 light screen 2 slidable, light screen 2 is used for sheltering from the lateral wall that is equipped with the casing 1 of stretching hand mouth 11. The detection assembly 3 comprises a first detection piece 31, a second detection piece 32 and a third detection piece 33, the first detection piece 31 is arranged on the side wall of the shell 1 matched with the light shielding plate 2, the first detection piece 31 is configured to detect whether the light shielding plate 2 seals the hand stretching opening 11, the number of the second detection pieces 32 is three, each second detection piece 32 is arranged on the inner wall of one hand stretching opening 11, and the second detection piece 32 is configured to detect whether the arm of the experimental animal passes through the hand stretching opening 11. The number of the third detecting members 33 is three, each third detecting member 33 is disposed corresponding to one feeding port 61, and the third detecting members 33 are used for detecting whether the arm of the experimental animal passes through the feeding port 61. The control assembly comprises a first timer and a second timer, the first timer records that the first time length from the time when the first detection piece 31 detects that the light shielding plate 2 is separated from the side wall of the shell 1 to the time when the second detection piece 32 detects the arm of the experimental animal for the first time is the first time length, and the second timer records that the time length from the time when the second detection piece 32 detects the arm of the experimental animal for the first time to the time when the second detection piece 32 detects the arm of the experimental animal for the second time is the second time length.
The experimental process of the experimental device for matching the auditory sense of the animal in the embodiment is as follows: the sounding subassembly sends and demolishs cooperation light screen 2 on the lateral wall of casing 1 after the prompt tone, and first time-recorder begins the timing after first detection piece 31 detects light screen 2 and is demolishd, if the length of time of first time-recorder record is greater than first predetermined length of time after, the experiment stops if the second detection piece 32 has not detected experimental animals' arm yet. In the time period that the duration recorded by the first timer is not more than the first preset duration, if the second detection part 32 detects the arm of the experimental animal for the first time, the first timer stops timing, the second timer starts timing, and if the duration recorded by the second timer is more than the second preset duration, the third detection part 33 does not detect the arm of the experimental animal yet, the experiment stops. And in a time period when the duration recorded by the second timer is not more than a second preset duration, the third detection piece 33 detects the arm of the experimental animal, records the experimental result, and keeps timing for the second timer until the second detection piece 32 detects that the arm of the experimental animal stops the experiment for the second time.
The behavioral experimental method for auditory association matching of animals according to the embodiment of the present invention will be described with reference to fig. 2.
As shown in fig. 2, the behavioral experimental method for matching the animal auditory association is performed by using the above-mentioned behavioral experimental apparatus for matching the animal auditory association, and the behavioral experimental method for matching the animal auditory association comprises the following steps;
s1: the sounding component gives out prompt sound;
s2: removing the light shielding plate 2 fitted on the side wall of the housing 1;
s3: recording the first time length from the removal of the light screen 2 to the extension of the arm of the experimental animal into the hand opening 11;
s4: recording a second time period from the arm of the experimental animal extending into the hand extending port 11 to the arm of the experimental animal exiting the hand extending port 11.
It can be understood that, in the experimental process, because the first time length from the removal of the light screen 2 to the extension of the arm of the experimental animal into the hand opening 11 and the second time length from the extension of the arm of the experimental animal into the hand opening 11 to the exit of the arm of the experimental animal from the hand opening 11 can be recorded, the experimenter can preliminarily analyze the reaction capability and the auditory association matching capability of the experimental animal according to the length of the first time length, and the execution capability of the experimental animal can be further measured according to the length of the second time length. Therefore, the behavioral experiment of the animal auditory sense association matching is divided into two time periods for analysis, the purpose of carrying out standardized division and quantitative judgment on the behaviors of the experimental animals is achieved, the association matching relation between the auditory sense and the behaviors of the animal can be analyzed in a quantitative mode by the experimental method, and the functions of the temporal lobe of the brain area can be analyzed more accurately.
According to the animal auditory association matching behavioural experiment method provided by the embodiment of the invention, the behavioural experiment matched with the animal auditory association is divided into the first time period from the removal of the light screen 2 to the extension of the arm of the experimental animal into the hand-stretching opening 11 and the second time period from the extension of the arm of the experimental animal into the hand-stretching opening 11 to the exit of the arm of the experimental animal from the hand-stretching opening 11, so that the aim of carrying out standardized segmentation and quantitative judgment on the behavior of the experimental animal is fulfilled, the experimental method can better quantitatively analyze the association matching relation between the auditory sense and the behavior of the animal, and the function of the temporal lobe of the brain area is more accurately analyzed.
In some embodiments, in step S2, after the light shielding plate 2 is removed for the first preset time, the arm of the experimental animal still does not pass through the hand stretching opening 11, and the experiment is stopped; in step S3, after the arm of the experimental animal passes through the hand stretching opening 11 for a second preset time, the arm of the experimental animal does not pass through the hand stretching opening again, and the experiment is stopped. It can be understood that, in the experiment process, the experimental animal can accurately take out food from the food cavity 13 corresponding to the experiment cavity 12 with the same number of times of the prompt tone, the execution of the experimental animal is calibrated to be successful, if the experimental animal extends an arm into the wrong experiment cavity 12, the execution of the experimental animal is calibrated to be failed, and if the experimental animal does not carry out the eating operation after the sounding component sends the prompt tone for a period of time, the non-execution behavior of the experimental animal is calibrated. However, if the experimental animal is always waiting for the completion of the food taking operation, the experimental animal may not extend the arm into the hand stretching opening 11 in some cases due to the difference of the auditory association matching ability and the execution ability of the experimental animal, and much time is wasted if the experiment is not stopped. In the embodiment of the invention, the first preset time and the second preset time are set as the nodes for forcibly stopping the experiment, so that the experiment time is saved, and the experiment efficiency is improved.
In the description herein, references to the description of "some embodiments," "other embodiments," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The above description is only a preferred embodiment of the present invention, and for those skilled in the art, the present invention should not be limited by the description of the present invention, which should be interpreted as a limitation.

Claims (10)

1.一种动物听觉联想匹配的行为学实验装置,其特征在于,包括:1. a behavioral experimental device of animal auditory association matching, is characterized in that, comprises: 壳体(1),所述壳体(1)内设有多个隔板(5),多个所述隔板(5)将所述壳体(1)内的空间分隔出多个腔体,每个所述腔体内均设有分割板(6),所述分割板(6)将所述腔体分隔为实验腔体(12)和食物腔体(13),所述壳体(1)上设有多个伸手口(11),每个所述伸手口(11)连通一个所述实验腔体(12),所述分割板(6)上设有取食口(61)以连通所述实验腔体(12)和所述食物腔体(13);A casing (1), a plurality of partitions (5) are arranged in the casing (1), and the plurality of partitions (5) divide the space in the casing (1) into a plurality of cavities , each cavity is provided with a dividing plate (6), the dividing plate (6) divides the cavity into an experimental cavity (12) and a food cavity (13), the shell (1) ) is provided with a plurality of mouths (11), each of the mouths (11) communicates with one of the experimental chambers (12), and the dividing plate (6) is provided with a feeding mouth (61) to communicate with the experimental cavity (12) and the food cavity (13); 遮光板(2),所述遮光板(2)可滑动地设在所述壳体(1)上,所述遮光板(2)用于遮挡设有多个所述伸手口(11)的所述壳体(1)的侧壁;A light-shielding plate (2), the light-shielding plate (2) is slidably arranged on the housing (1), and the light-shielding plate (2) is used for shielding all the places provided with a plurality of the hand openings (11). the side wall of the casing (1); 检测组件(3),所述检测组件(3)被配置为检测所述遮光板(2)是否封闭所述伸手口(11)、检测实验动物的手臂是否穿过所述伸手口(11);a detection component (3), the detection component (3) is configured to detect whether the visor (2) closes the hand-reaching opening (11), and to detect whether the arm of the experimental animal passes through the hand-reaching opening (11); 翻板(4),所述翻板(4)为多个,每个所述翻板(4)对应一个所述取食口(61)设置;a flap (4), the flaps (4) are multiple, and each of the flaps (4) is provided corresponding to one of the food intake ports (61); 发声组件,所述发声组件用于朝向所述实验动物发出声音刺激;a sound-generating component, the sound-generating component is used to emit sound stimulation towards the experimental animal; 控制组件,所述控制组件与所述发声组件及所述检测组件(3)电连接,所述控制组件被配置为记录拆除所述遮光板(2)到所述实验动物的手臂伸入所述伸手口(11)的第一时长以及记录所述实验动物的手臂伸入所述伸手口(11)到所述实验动物的手臂退出所述伸手口(11)的第二时长。A control assembly, the control assembly is electrically connected with the sounding assembly and the detection assembly (3), the control assembly is configured to record the removal of the light shield (2) to the arm of the experimental animal extending into the The first duration of the hand-reaching mouth (11) and the second duration of recording the experimental animal's arm extending into the hand-reaching mouth (11) to the experimental animal's arm exiting the hand-reaching mouth (11). 2.根据权利要求1所述的动物听觉联想匹配的行为学实验装置,其特征在于,所述检测组件(3)包括:2. The behavioral experiment device of animal auditory association matching according to claim 1, is characterized in that, described detection component (3) comprises: 第一检测件(31),所述第一检测件(31)设在所述壳体(1)的配合有所述遮光板(2)的侧壁上,所述第一检测件(31)被配置检测所述遮光板(2)是否封闭所述伸手口(11);A first detection piece (31), the first detection piece (31) is provided on the side wall of the casing (1) on which the light shielding plate (2) is fitted, and the first detection piece (31) being configured to detect whether the visor (2) closes the hand opening (11); 第二检测件(32),所述第二检测件(32)为多个,每个所述第二检测件(32)设在一个所述伸手口(11)的内壁上,所述第二检测件(32)被配置检测所述实验动物的手臂是否穿过所述伸手口(11)。The second detection member (32), there are multiple second detection members (32), each of the second detection members (32) is arranged on the inner wall of the hand opening (11), the second detection member (32) The detection part (32) is configured to detect whether the arm of the experimental animal passes through the hand opening (11). 3.根据权利要求2所述的动物听觉联想匹配的行为学实验装置,其特征在于,所述控制组件包括:3. the behavioral experiment device of animal auditory association matching according to claim 2, is characterized in that, described control assembly comprises: 第一计时器,所述第一计时器记录所述第一检测件(31)检测到所述遮光板(2)与所述壳体(1)的侧壁分离到所述第二检测件(32)第一次检测到所述实验动物的手臂的时长为所述第一时长;A first timer, the first timer records that the first detection member (31) detects that the light shielding plate (2) is separated from the side wall of the housing (1) to the second detection member ( 32) the time length of detecting the arm of the experimental animal for the first time is the first time length; 第二计时器,所述第二计时器记录所述第二检测件(32)第一次检测到所述实验动物的手臂到所述第二检测件(32)第二次检测到所述实验动物的手臂的时长为所述第二时长。A second timer, which records the first detection of the arm of the experimental animal by the second detection piece (32) to the second detection of the experiment by the second detection piece (32) The duration of the animal's arm is the second duration. 4.根据权利要求3所述的动物听觉联想匹配的行为学实验装置,其特征在于,所述控制组件包括归零按钮(7),所述归零按钮(7)与所述第一计时器、第二计时器、第二检测件(32)电连接。4. The behavioral experiment device for animal auditory association matching according to claim 3, wherein the control component comprises a reset button (7), the reset button (7) and the first timer , the second timer, and the second detection element (32) are electrically connected. 5.根据权利要求1所述的动物听觉联想匹配的行为学实验装置,其特征在于,所述检测组件(3)还包括:第三检测件(33),所述第三检测件(33)为多个,每个所述第三检测件(33)对应一个所述取食口(61)设置,所述第三检测件(33)用于检测所述实验动物的手臂是否穿过所述取食口(61)。5. The behavioral experimental device for animal auditory association matching according to claim 1, wherein the detection component (3) further comprises: a third detection part (33), the third detection part (33) There are multiple ones, each of the third detection parts (33) is provided corresponding to one of the feeding openings (61), and the third detection parts (33) are used to detect whether the arm of the experimental animal passes through the Feeding port (61). 6.根据权利要求5所述的动物听觉联想匹配的行为学实验装置,其特征在于,所述检测组件(3)还包括指示灯,所述指示灯为多个,多个所述指示灯与多个所述第三检测件(33)一一对应设置,每个所述指示灯与所述第三检测件(33)电连接。6. The behavioral experiment device of animal auditory association matching according to claim 5, is characterized in that, described detection assembly (3) also comprises indicator light, and described indicator light is a plurality, and a plurality of described indicator lights and A plurality of the third detection pieces (33) are arranged in a one-to-one correspondence, and each of the indicator lights is electrically connected to the third detection piece (33). 7.根据权利要求1所述的动物听觉联想匹配的行为学实验装置,其特征在于,所述壳体(1)的设有所述伸手口(11)的侧壁上设有两个间隔设置的滑槽,所述遮光板(2)的相对设置的两个侧边配合在两个所述滑槽内。7. The behavioral experiment device of animal auditory association matching according to claim 1, wherein two spaced settings are provided on the side wall of the housing (1) that is provided with the hand opening (11). The two opposite sides of the shading plate (2) are matched in the two sliding grooves. 8.根据权利要求1所述的动物听觉联想匹配的行为学实验装置,其特征在于,所述腔体为至少三个。8 . The behavioral experimental device for animal auditory association matching according to claim 1 , wherein there are at least three cavities. 9 . 9.一种动物听觉联想匹配的行为学实验方法,其特征在于,所述动物听觉联想匹配的行为学实验方法采用如权利要求1-8中任一项所述动物听觉联想匹配的行为学实验装置进行,所述动物听觉联想匹配的行为学实验方法包括如下步骤;9. a behavioral experiment method of animal auditory association matching is characterized in that, the behavioral experiment method of described animal auditory association matching adopts the behavioral experiment of animal auditory association matching as described in any one of claims 1-8 The device is carried out, and the behavioral experimental method of the animal auditory association matching includes the following steps; S1:所述发声组件发出提示音;S1: the sounding component emits a prompt sound; S2:拆除配合在所述壳体(1)的侧壁上的所述遮光板(2);S2: remove the light shielding plate (2) fitted on the side wall of the housing (1); S3:记录拆除所述遮光板(2)到所述实验动物的手臂伸入所述伸手口(11)的第一时长;S3: record the first time period from when the light shielding plate (2) is removed to when the arm of the experimental animal extends into the hand opening (11); S4:记录所述实验动物的手臂伸入所述伸手口(11)到所述实验动物的手臂退出所述伸手口(11)的第二时长。S4: Record the second time period from when the arm of the experimental animal extends into the hand-reaching opening (11) to when the experimental animal's arm exits the hand-reaching mouth (11). 10.根据权利要求9所述的动物听觉联想匹配的行为学实验方法,其特征在于,在步骤S3中,拆除所述遮光板(2)第一预设时长后,所述实验动物的手臂仍然没有穿过所述伸手口(11),则实验停止;10. The behavioral experiment method of animal auditory association matching according to claim 9, characterized in that, in step S3, after removing the first preset duration of the visor (2), the arm of the experimental animal is still If it does not pass through the hand opening (11), the experiment stops; 在步骤S4中,所述实验动物的手臂穿过所述伸手口(11)第二预设时长后,所述实验动物的手臂没有再次穿过所述伸手口(11),则实验停止。In step S4, after the arm of the experimental animal passes through the hand extension port (11) for a second preset period of time, the experiment stops if the arm of the experimental animal does not pass through the hand extension port (11) again.
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Publication number Priority date Publication date Assignee Title
CN110839558B (en) * 2019-11-19 2021-07-06 中国科学院深圳先进技术研究院 A behavioral experimental device and experimental method for animal auditory association matching
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Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0816986A2 (en) * 1996-07-03 1998-01-07 Hitachi, Ltd. Method, apparatus and system for recognizing motions
CN1817325A (en) * 2006-02-21 2006-08-16 沈阳医学院 Mouse hearing and vision memory model and intelligent action inspecting system
CN105010164A (en) * 2015-07-14 2015-11-04 湖北第二师范学院 IOT mouse behavior training device
CN204968904U (en) * 2015-09-30 2016-01-20 广西南宁灵康赛诺科生物科技有限公司 A testing arrangement for primate
CN105475163A (en) * 2015-09-30 2016-04-13 广西南宁灵康赛诺科生物科技有限公司 Method for testing memory, judgment and execution capabilities of primate
CN109220875A (en) * 2018-08-23 2019-01-18 浙江大学 A kind of automated system for the training of macaque Auditory behavior
CN109566447A (en) * 2018-12-07 2019-04-05 中国人民解放军军事科学院军事医学研究院 The research system of non-human primate movement and cognitive function based on touch screen
CN110122366A (en) * 2019-07-02 2019-08-16 淮阴师范学院 A kind of learning and memory of little mouse behaviouristics training test device
CN110278884A (en) * 2019-06-14 2019-09-27 中国科学院深圳先进技术研究院 Animal instinct fear behavioral value device and detection method

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7263953B2 (en) * 2005-03-30 2007-09-04 Krishnamurthy Sundararajan Automatic pet trainer
CN104663490B (en) * 2015-03-03 2018-10-09 广西南宁灵康赛诺科生物科技有限公司 The automatic device of a set of evaluation primate laboratory animal memory function and the cognitive ability evaluation method of animal
CN110012844B (en) * 2019-04-10 2021-05-18 中国科学院深圳先进技术研究院 Underwater multifunctional integrated animal experiment equipment
CN110301363B (en) * 2019-06-24 2021-07-09 中国科学技术大学 A kind of animal cognitive behavior test method
CN110833047B (en) * 2019-11-19 2021-08-31 中国科学院深圳先进技术研究院 A behavioral experimental device and experimental method for animal visuospatial cognitive memory
CN110839558B (en) * 2019-11-19 2021-07-06 中国科学院深圳先进技术研究院 A behavioral experimental device and experimental method for animal auditory association matching
CN110786250B (en) * 2019-11-19 2021-08-31 中国科学院深圳先进技术研究院 A behavioral experimental device and experimental method for animal search memory

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0816986A2 (en) * 1996-07-03 1998-01-07 Hitachi, Ltd. Method, apparatus and system for recognizing motions
CN1817325A (en) * 2006-02-21 2006-08-16 沈阳医学院 Mouse hearing and vision memory model and intelligent action inspecting system
CN105010164A (en) * 2015-07-14 2015-11-04 湖北第二师范学院 IOT mouse behavior training device
CN204968904U (en) * 2015-09-30 2016-01-20 广西南宁灵康赛诺科生物科技有限公司 A testing arrangement for primate
CN105475163A (en) * 2015-09-30 2016-04-13 广西南宁灵康赛诺科生物科技有限公司 Method for testing memory, judgment and execution capabilities of primate
CN109220875A (en) * 2018-08-23 2019-01-18 浙江大学 A kind of automated system for the training of macaque Auditory behavior
CN109566447A (en) * 2018-12-07 2019-04-05 中国人民解放军军事科学院军事医学研究院 The research system of non-human primate movement and cognitive function based on touch screen
CN110278884A (en) * 2019-06-14 2019-09-27 中国科学院深圳先进技术研究院 Animal instinct fear behavioral value device and detection method
CN110122366A (en) * 2019-07-02 2019-08-16 淮阴师范学院 A kind of learning and memory of little mouse behaviouristics training test device

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