CN110278884A - Animal instinct fear behavioral value device and detection method - Google Patents

Abstract

The invention provides an animal instinctive fear behavior detection device and a detection method. The animal instinct fear behavior detection device includes: a box body, which includes a behavior test box for animals to perform instinct fear behavior tests and a box for Animals dodge after making an instinctive fear response, and an opening for communicating between the behavior test box and the avoidance box is provided between the behavior test box and the avoidance box; the visual stimulation device is used to treat animals Stimulating signals are sent out to stimulate the animal to react with instinctive fear; and a video camera is arranged above the box for recording the behavioral response of the animal. The animal instinctive fear behavior detection device and detection method provided by the present invention can accurately obtain the movement path and movement speed of the animal, and effectively improve the reliability and stability of the experimental results.

Description

Animal instinctive fear behavior detection device and detection method

technical field

The invention belongs to the technical field of animal behavior, and more specifically relates to an animal instinctive fear behavior detection device and detection method.

Background technique

As one of the six basic emotional behaviors, fear is induced by the subject's perception of danger. The defensive behavior generated by fear is an important guarantee for the survival and reproduction of animals, and is the most important and conservative part in the evolution of species.

Disorders of fear regulation may contribute to a range of related disorders, such as panic disorder and specific phobias. The research on the visceral fear circuit will help to find the mechanism of this kind of mental illness and develop new diagnosis and treatment strategies. Animal models used in circuit research, especially mouse models, are widely used to study mental illness. For mice, avoiding aerial predators, such as eagles, is an important survival ability. May be mediated by specific neural circuits.

At present, the devices used to study the instinctive fear behavior of mice in the laboratory usually place shelters in the boxes, which makes it difficult for the mice to film their behavior after escaping into the shelters. In order to capture the environment in the shelters in the prior art, cameras usually need Shooting from the side resulted in severe distortion of the picture, making it impossible to know the exact path of the mouse's movement, and it was also impossible to analyze the speed of the mouse's movement.

Contents of the invention

The object of the present invention is to provide an animal instinctive fear behavior detection device to solve the technical problem in the prior art that the animal's movement path cannot be accurately obtained.

In order to achieve the above object, the technical solution adopted by the present invention is to provide an animal instinctive fear behavior detection device, comprising:

A box body, the box body includes a behavior test box for the animal to carry out the instinctive fear behavior test and an avoidance box for the animal to avoid after making an instinctive fear response, between the behavior test box and the avoidance box An opening for connecting the behavior test box and the avoidance box is provided;

a visual stimulation device for signaling an animal to stimulate a visceral fear response; and,

A video camera is arranged above the box for recording the animal's behavioral responses.

Further, the cross-sectional shape of the side wall of the behavior test box is circular or oval.

Further, the visual stimulation device is arranged above or around the behavior test box.

Further, the visual stimulation device is a display screen.

Further, the camera is arranged above the opening.

Further, the animal instinct fear behavior detection device also includes a partition, and the avoidance box is provided with a plurality of clips for fixing the partition at intervals along the direction away from the behavior test box, and the partition is detachable. Install in any one of the first card slots.

Further, the side edge of the opening is provided with a second locking position for fixing the partition.

Further, the animal instinct fear behavior detection device also includes a bracket for supporting the visual stimulation device and the camera, and the bracket is arranged outside the box.

Further, the outer periphery of the behavior test box is provided with an outer wall for light-shielding and sound-proofing.

The present invention also provides a method for detecting instinctive fear behavior of animals, comprising the following steps:

Place the animal in the box of the animal instinctive fear behavior detection device as described in any one of the above;

The visual stimulation device sends a stimulation signal to the animal, and the animal makes an instinctive fear response after receiving the stimulation signal;

A video camera records the animal's behavioral responses.

The beneficial effects of the air-conditioned animal instinct fear behavior detection device and detection method provided by the present invention are: compared with the prior art, the animal instinct fear behavior detection device of the present invention, by setting the behavior test box and the avoidance box, the behavior test box and avoidance There is an opening between the boxes for connecting the behavior test box and the avoidance box, so that the camera can be set above the box, so that the camera can shoot from above, so that the deformation of the shooting picture is reduced, and the movement path of the animal can be accurately obtained And the speed of movement effectively improves the reliability and stability of the experimental results, and avoids the prior art because the camera can only shoot from the side due to the shelter set in the box, resulting in the deformation of the shooting picture and the inability to obtain the exact movement path of the animal.

Description of drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following will briefly introduce the accompanying drawings that need to be used in the descriptions of the embodiments or the prior art. Obviously, the accompanying drawings in the following description are only of the present invention. For some embodiments, those of ordinary skill in the art can also obtain other drawings based on these drawings without paying creative efforts.

FIG. 1 is a schematic diagram of the three-dimensional structure of an animal instinctive fear behavior detection device provided by an embodiment of the present invention;

FIG. 2 is a schematic diagram of a three-dimensional structure of a box provided by an embodiment of the present invention;

Fig. 3 is a three-dimensional structural schematic diagram of an animal instinctive fear behavior detection device provided by another embodiment of the present invention.

Wherein, each reference sign in the figure:

10-box; 11-behavior test box; 12-avoid box; 13-opening; 20-camera; 30-partition; 40-bracket; 50-external wall.

Detailed ways

In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

It should be noted that when an element is referred to as being “fixed” or “disposed on” another element, it may be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element.

It is to be understood that the terms "length", "width", "top", "bottom", "front", "rear", "left", "right", "vertical", "horizontal", "top" , "bottom", "inner", "outer" and other indicated orientations or positional relationships are based on the orientations or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying the referred device Or elements must have a certain orientation, be constructed and operate in a certain orientation, and thus should not be construed as limiting the invention.

In addition, the terms "first" and "second" are used for descriptive purposes only, and cannot be interpreted as indicating or implying relative importance or implicitly specifying the quantity of indicated technical features. Thus, a feature defined as "first" and "second" may explicitly or implicitly include one or more of these features. In the description of the present invention, "plurality" means two or more, unless otherwise specifically defined.

Please refer to FIG. 1 and FIG. 2 together, and now the animal instinctive fear behavior detection device provided by the present invention will be described. The present invention provides an animal instinctive fear behavior detection device, which includes a box 10 , a visual stimulation device (not shown in the figure) and a camera 20 . The visual stimulation device is used to send a stimulation signal to the animal to stimulate the animal's instinctive fear response. Wherein, the present embodiment selects mouse to carry out experiment. The video camera 20 is arranged on the top of the box 10, and is used for recording the behavioral responses of the animals. The box body 10 includes a behavior test box 11 and an avoidance box 12, and the behavior test box 11 is used for the animal to conduct the instinctive fear behavior test. The dodge box 12 is used for the animal to hide after making an instinctive fear response. An opening 13 for connecting the behavior testing box 11 and the avoiding box 12 is provided between the behavior testing box 11 and the avoiding box 12. When the animal is stimulated by the visual stimulation device, it will react instinctively and enter the avoiding box through the opening 13. Dodge within 12. In the present invention, the behavior test box 11 and the avoidance box 12 are separately arranged, so that the camera 20 can shoot from above, thereby making the shooting picture clear, and avoiding the fact that the camera 20 in the prior art can only shoot because of the shelter 10 provided in the box body 10. Shooting from the side distorts the shot, making it impossible to capture the exact path of the animal's movement.

Compared with the prior art, the animal instinct fear behavior detection device provided by the present invention, by setting the behavior test box 11 and the avoidance box 12, is provided with for connecting the behavior test box 11 and the avoidance box between the behavior test box 11 and the avoidance box 12 The opening 13 of 12 enables the camera 20 to be arranged above the box body 10, so that the camera 20 can shoot from above, so that the shape of the shooting picture is reduced, and the movement path and speed of the animal can be accurately obtained, effectively improving the experimental results. The reliability and stability of the camera 20 can only be taken from the side due to the shields installed in the box body 10 in the prior art, resulting in deformation of the shooting picture and the inability to obtain the exact movement path of the animal.

Further, referring to FIG. 1 and FIG. 2 , as a specific embodiment of the animal instinctive fear behavior detection device provided by the present invention, the cross-sectional shape of the side wall of the behavior testing box 11 is circular. Since the behavioral testing device of traditional technology usually adopts a cuboid or cube box with four corners, it is possible for the mice to regard the corners as a safe area when they face the shadow of a simulated natural enemy, thus introducing a negative effect on the output of instinctive fear behavior. Uncertain factors lead to inaccurate test results. And the present invention is by setting the cross-sectional shape of the side wall of the behavior testing box 11 to be circular, can not have the problem of the corner, has improved the stability and reliability of the experiment, has solved the prior art because adopting the box body of square to cause four sides There are corners between the side walls, which makes it easy for animals to regard the corners between the side walls as safe areas, resulting in inaccurate detection results. It should be noted that the cross-sectional shape of the side wall of the behavior test box 11 is not limited thereto, for example, in other preferred implementations of the present invention, the cross-sectional shape of the side wall of the behavior test box 11 can also be oval .

Further, as a specific embodiment of the animal instinctive fear behavior detection device provided by the present invention, the visual stimulation device is arranged above the behavior test box 11, so as to detect the mouse's response to the upper visual field visual danger stimulus. The stimulating signal sent by the visual stimulating device can be a black disc. It should be noted that the cross-sectional shape of the side wall of the behavior test box 11 is not limited thereto, for example, in other preferred implementations of the present invention, the visual stimulation device can also be arranged on the peripheral side of the behavior test box 11, The behavioral responses of mice to visual stimuli from around them were thus detected. Preferably, the visual stimulation device is a display screen. Display the black disc by utilizing the display.

Further, referring to FIG. 1 and FIG. 2 , as a specific embodiment of the animal instinctive fear behavior detection device provided by the present invention, the camera 20 is arranged above the opening 13 . By installing the camera 20 above the opening 13, it is convenient for the camera 20 to capture all of the behavior test box 11 and the avoidance box 12, and there is no shooting blind spot. Preferably, the camera 20 is a wide-angle camera. In order to prevent the visual stimulation device from blocking the shooting of the camera 20, the height of the camera 20 should be lower than that of the visual stimulation device.

Further, referring to Fig. 1 and Fig. 2, as a specific embodiment of the animal instinctive fear behavior detection device provided by the present invention, the animal instinct fear behavior detection device also includes a partition 30, and the avoidance box 12 is along the side far away from the behavior test box 11. A plurality of first card positions (not shown) are arranged at intervals in the direction, wherein the avoidance box 12 refers to the length direction of the avoidance box 12 along the direction away from the behavior test box 11 in this embodiment, and the first card positions can be used for fixing the spacer. The board 30 is detachably installed in any one of the first card positions, so that the dodging box 12 can be divided into different lengths, and the length of the dodging box 12 can be changed according to actual needs. Specifically, the first locking position can be a concave position provided on the side wall of the dodge box, and it is only necessary to place the partition board in the concave position, and the operation is very simple and convenient.

Further, referring to Fig. 1 and Fig. 2, as a kind of embodiment of the animal instinctive fear behavior detection device provided by the present invention, the side edge of opening 13 is provided with the second stop (not shown in the figure), the second stop Used to fix the partition 30. When the dividing plate 30 is clamped on the second card position, the dividing plate 30 isolating the avoidance box 12 from the behavior test box at this moment, and the mice can only move in the behavior test box 11 and cannot enter the avoidance box 12, thereby It can be compared with the situation where there is a dodge box 12 .

Further, as a specific embodiment of the animal instinct fear behavior detection device provided by the present invention, the animal instinct fear behavior detection device also includes a bracket 40 for supporting the visual stimulation device and the camera 20, and the bracket 40 is arranged on the box body 10 In addition, the visual stimulation device and the camera 20 can be fixed above the box body 10 through the setting of the bracket 40 .

Further, referring to Fig. 3, as a specific embodiment of the animal instinctive fear behavior detection device provided by the present invention, the outer periphery of the behavior test box 11 is provided with an outer wall 50, and the outer wall 50 is used for light-shielding and sound insulation, so that the experiment Conditions are stable.

Further, referring to Fig. 1, as a kind of embodiment of the animal instinctive fear behavior detection device provided by the present invention, the height of box body 10 is 25cm-35cm, is preferably 30cm, thereby not only can effectively prevent mouse from escaping, but also Mice can be guaranteed to have a sufficiently wide upper field of view. The behavior test box 11 has a diameter of 45cm-55cm, preferably 50cm. The dodging box 12 is a cuboid structure. The dodging box 12 has a length of 45cm-55cm and a width of 5cm-15cm. For example, in one embodiment of the present invention, the dodging box 12 has a length of 50cm and a width of 10cm.

Further, as a specific embodiment of the animal instinctive fear behavior detection device provided by the present invention, the box 10 is made of acrylic board, and acrylic is light in weight, cheap, non-toxic, easy to form, and has good acid and alkali resistance. Etc. Certainly, the material of the box body 10 is not limited thereto, for example, in other preferred embodiments of the present invention, the box body 10 can also be a thin metal plate. Specifically, the color of the casing 10 can be changed according to the fur color of the mouse studied. For example, when the fur color of the mouse is black, the bottom plate color of the casing 10 can be white, for example, it can be a white frosted bottom plate. When the coat color of the mouse was white, the bottom plate color of the casing 10 could be black, so as to achieve the best shooting effect. Preferably, when it is necessary to shoot and record the behavior of the mouse from the side, a side plate made of transparent acrylic can also be used, so that the camera can record the movement behavior of the mouse from all around.

The present invention also provides a method for detecting instinctive fear behavior of animals, comprising the following steps:

S10, placing the animal in the box 10 of the above-mentioned animal instinctive fear behavior detection device.

S20. The visual stimulation device sends a stimulation signal to the animal, and the animal makes an instinctive fear response after receiving the stimulation signal.

S30. The camera 20 records the animal's behavioral response.

In the present invention, the behavior test box 11 and the avoidance box 12 are arranged, and an opening 13 for connecting the behavior test box 11 and the avoidance box 12 is arranged between the behavior test box 11 and the avoidance box 12, so that the camera 20 can be arranged on the top of the box body 10 , so that the camera 20 can shoot from above, so that the shape of the shooting picture becomes smaller, and then the moving path and moving speed of the animal can be accurately obtained, the reliability and stability of the experimental results are effectively improved, and the prior art is avoided The occluder set in 10 causes the camera 20 to only shoot from the side, causing the shooting picture to be deformed, thus making it impossible to obtain the exact movement path of the animal.

The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention should be included in the protection of the present invention. within range.

Claims (10)

1. The animal instinct fear behavior detection device is characterized in that: comprising: A box body, the box body includes a behavior test box for the animal to carry out the instinctive fear behavior test and an avoidance box for the animal to avoid after making an instinctive fear response, between the behavior test box and the avoidance box An opening for connecting the behavior test box and the avoidance box is provided; a visual stimulation device for signaling an animal to stimulate a visceral fear response; and, A video camera is arranged above the box for recording the animal's behavioral responses. 2. The animal instinct fear behavior detection device according to claim 1, characterized in that: the cross-sectional shape of the side wall of the behavior test box is circular or oval. 3 . The animal instinctive fear behavior detection device according to claim 1 , wherein the visual stimulation device is arranged above or around the behavior testing box. 4 . 4. The animal instinct fear behavior detection device according to claim 3, characterized in that: the visual stimulation device is a display screen. 5. The animal instinctive fear behavior detection device according to claim 1, characterized in that: the camera is arranged above the opening. 6. The animal instinct fear behavior detection device as claimed in any one of claims 1-5, characterized in that: the animal instinct fear behavior detection device also includes a partition, and the avoidance box is along the side of the behavior test box away from A plurality of clamping positions for fixing the partitions are provided at intervals in the direction, and the partitions are detachably installed in any one of the first clamping positions. 7. The animal instinct fear behavior detection device according to claim 6, characterized in that: the side edge of the opening is provided with a second locking position for fixing the partition. 8. The animal instinct fear behavior detection device according to any one of claims 1-6, characterized in that: the animal instinct fear behavior detection device also includes a support for supporting the visual stimulation device and the camera, The bracket is arranged outside the box. 9. The air-conditioning system according to any one of claims 1-6, characterized in that: the outer periphery of the behavior test box is provided with an outer wall for light-shielding and sound-proofing. 10. The detection method of animal instinct fear behavior, it is characterized in that: comprise the following steps: The animal is placed in the casing of the animal instinct fear behavior detection device described in any one of claims 1-9; The visual stimulation device sends a stimulation signal to the animal, and the animal makes an instinctive fear response after receiving the stimulation signal; A video camera records the animal's behavioral responses.