CN110786250B - A behavioral experimental device and experimental method for animal search memory - Google Patents
A behavioral experimental device and experimental method for animal search memory Download PDFInfo
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- CN110786250B CN110786250B CN201911134324.3A CN201911134324A CN110786250B CN 110786250 B CN110786250 B CN 110786250B CN 201911134324 A CN201911134324 A CN 201911134324A CN 110786250 B CN110786250 B CN 110786250B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/03—Housing for domestic or laboratory animals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01K1/00—Housing animals; Equipment therefor
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- A01K1/035—Devices for use in keeping domestic animals, e.g. fittings in housings or dog beds
- A01K1/0356—Feeding or drinking devices associated with cages
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K29/00—Other apparatus for animal husbandry
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
本发明公开了一种动物搜索记忆的行为学实验装置及实验方法，其中动物搜索记忆的行为学实验装置包括壳体、对照遮光板、执行遮光板、检测组件、对照翻板、支撑翻板和控制组件。壳体内分隔设置有实验腔体和食物腔体，实验腔体分隔设置有对照腔体和执行腔体，对照腔体的底壁上设有多个对照取食口，执行腔体的底壁上设有多个执行取食口，壳体上设有两个伸手口，两个伸手口分别对应对照腔体和执行腔体设置，执行遮光板可滑动地设在壳体上，对照翻板为多个，每个对照翻板对应一个对照取食口设置，支撑翻板为多个，每个支撑翻板对应一个执行取食口设置。该行为学实验装置能够较好地量化动物搜索记忆的行为学能力，从而更为准确地分析对应脑区颞叶的功能。
The invention discloses a behavioral experimental device and an experimental method for animals to search for memory, wherein the behavioral experimental device for animals to search for memory comprises a casing, a control shading plate, an execution shading plate, a detection component, a control flap, a support flap and a control components. An experimental cavity and a food cavity are separated in the shell, and the experimental cavity is divided into a control cavity and an execution cavity. The bottom wall of the control cavity is provided with a plurality of control feeding ports, and the bottom wall of the execution cavity There are a plurality of execution food intake ports, and two hand-reaching ports are provided on the shell. The two hand-reaching ports are respectively set corresponding to the control cavity and the execution cavity. The execution shading plate is slidably arranged on the shell. A plurality of control flaps are set corresponding to one control feeding port, and there are multiple supporting flips, and each supporting flip is corresponding to one performing feeding port setting. The behavioral experimental device can better quantify the behavioral ability of animals to search memory, so as to more accurately analyze the function of the temporal lobe of the corresponding brain region.
Description
Technical Field
The invention relates to the technical field of animal behavioural research, in particular to an experimental device and an experimental method for animal search memory behavioural.
Background
With the development of scientific technology, animals have been widely used in medical biology (including behavioural) testing and research due to great resemblance in neuroanatomy, neurophysiology, immunology and genetic characteristics to humans. Behavioral training is the study of clinical characteristics of animals, and animals complete target tasks in training to achieve the purpose of evaluating certain indexes so as to reveal the essence and the law of life activities, health and diseases. However, it is difficult and complicated to deploy a reasonable behavioral apparatus because it is a large, smart, social, long-lived, non-domesticated, and offensive animal. The previous research on cognitive memory of each visual space focuses on the test of working memory.
At present, the prior art does not provide a behavioristics device capable of accurately quantifying animal search memory, most of the prior devices can only carry out qualitative analysis, the analysis result is not accurate, the relation between the auditory sensation and the behaviour of an animal can not be well reflected, and therefore the function of the temporal lobe of the brain area corresponding to the animal can not be well analyzed.
Disclosure of Invention
One objective of the present invention is to provide a behavioural test device for animal search memory, which can better quantify the behavioural ability of animal search memory, thereby more accurately analyzing the function of the corresponding temporal lobe of brain region.
One objective of the present invention is to provide a behavioral experimental method for animal memory search, which can better quantify the behavioral abilities of animal memory search, thereby more accurately analyzing the functions of the corresponding temporal lobe of brain region.
In order to achieve the technical effects, the technical scheme of the invention is as follows:
a behavioural experimental device for animal memory search, comprising: the food taking device comprises a shell, an experiment cavity and a food cavity are separately arranged in the shell, a control cavity and an execution cavity are separately arranged in the experiment cavity, a plurality of control food taking ports are arranged on the bottom wall of the control cavity and are respectively communicated with the food cavity, a plurality of execution food taking ports are arranged on the bottom wall of the execution cavity and are respectively communicated with the food cavity, two hand stretching ports are arranged on the shell and respectively correspond to the control cavity and the execution cavity; the contrast light shielding plate is arranged on the shell in a sliding mode and used for shielding the contrast cavity; the execution light shielding plate is arranged on the shell in a sliding mode and used for shielding the execution cavity; a detection component configured to detect whether the control shield obstructs the control cavity, detect whether the execution shield obstructs the execution cavity, detect whether an arm of an experimental animal passes through the hand-stretching mouth, and detect whether an arm of the experimental animal passes through the execution food-taking mouth; the control turning plates are multiple, each control turning plate corresponds to one control food taking opening and one of the control turning plates is different from the other control turning plates in color; the executing turning plates are multiple, each executing turning plate corresponds to one executing food taking port, the colors of the executing turning plates are the same, and the distribution mode of the executing turning plates is the same as the distribution direction of the comparison turning plates; the control assembly comprises a timing unit, and the timing unit is configured to record a first time length from the removal of the control light shielding plate to the removal of the execution light shielding plate, record a second time length from the removal of the execution light shielding plate to the extension of the arm of the experimental animal into the hand stretching port, and record a third time length from the extension of the arm of the experimental animal into the hand stretching port to the extension of the arm of the experimental animal into the food taking port.
In some embodiments, the detection component comprises: the first detection pieces are respectively arranged on the outer side walls of the comparison cavity and the execution cavity, one of the first detection pieces is configured to detect whether the execution light shielding plate shields the execution cavity, and the other one of the first detection pieces is configured to detect whether the comparison light shielding plate shields the comparison cavity; the number of the second detection pieces is two, each second detection piece is arranged on the inner wall of one hand stretching opening, and the second detection pieces are configured to detect whether the arms of the experimental animal pass through the hand stretching openings or not; the number of the third detecting pieces is multiple, each third detecting piece is arranged on the inner wall of one executing food taking opening, and the third detecting pieces are configured to detect whether the arms of the experimental animals penetrate through the executing food taking openings.
In some specific embodiments, the timing unit includes: a first timer which records a period of time from when one of the first detecting members detects the separation of the control shutter plate from the control chamber to when the other of the first detecting members detects the separation of the implement shutter plate from the implement chamber as the first period of time; the second timer records that the time length from the first detection piece detecting that the execution light shielding plate is separated from the execution cavity to the second detection piece detecting that the arm of the experimental animal passes through the hand stretching opening is a second time length; and the third timer records that the time from the time that the second detection piece detects that the arm of the experimental animal passes through the hand stretching port to the time that the third detection piece detects that the arm of the experimental animal passes through the food taking port is the third time.
In some specific embodiments, the control assembly includes a zero button electrically connected to the first, second, and third timers.
In some optional embodiments, the detection assembly further includes a plurality of indicator lights, the plurality of indicator lights are disposed in one-to-one correspondence with the plurality of third detection members, and each indicator light is electrically connected to the third detection member.
In some optional embodiments, the control food intake and the execute food intake are arranged in a row.
A behavioural experimental method for animal memory search is carried out by adopting the behavioural experimental device for animal memory search, and comprises the following steps;
s1: removing the control visor;
s2: removing the executing shutter plate and recording a first time period from the removal of the reference shutter plate to the removal of the executing shutter plate;
s3: recording a second time length from the execution light shielding plate is removed until the arm of the experimental animal extends into the hand extending opening;
s4: recording a third time period from the time when the arms of the experimental animals stretch into the hand stretching openings to the time when the arms of the experimental animals stretch into the food taking openings.
In some alternative embodiments, in step S2, the control shutter plate is installed while the implement shutter plate is removed to allow the shutter plate to occlude the control volume.
In some alternative embodiments, in step S3, after the executive visor is removed for the first preset time, the arm of the experimental animal still does not pass through the hand stretching mouth, and the experiment is stopped.
In some optional embodiments, in step S4, after the arm of the experimental animal passes through the hand-stretching opening for a second preset time, the arm of the experimental animal does not stretch into the feeding-taking opening, and the experiment is stopped.
According to the ethology experiment device for animal search memory, disclosed by the embodiment of the invention, the control assembly can record the first time from the removal of the control light screen to the removal of the execution light screen, the second time from the removal of the execution light screen to the extension of the arm of the experimental animal into the hand stretching port and the third time from the extension of the arm of the experimental animal into the execution food taking port, so that the whole ethology experiment for search memory is divided into three time periods of observing the distribution of the control turning plate, the extension of the arm into the hand stretching port and the extension of the arm into the execution food taking port, the animal behaviors of the experimental animal are standardized and accurately quantified, the whole experiment is universal, and the function of detecting the temporal lobe of the corresponding brain area is realized by the device designed by detecting the memory state of the animal.
According to the ethology experiment method for animal search memory, in the ethology experiment process, the first time length from the removal of the control light screen to the removal of the execution light screen, the second time length from the removal of the execution light screen to the extension of the arm of the experimental animal into the hand-stretching mouth and the third time length from the extension of the arm of the experimental animal into the hand-stretching mouth to the extension of the arm of the experimental animal into the food-taking mouth are recorded, the whole ethology experiment for search memory is divided into three time periods of observation of the distribution of the control turning plate, the extension of the arm into the hand-stretching mouth and the extension of the arm into the food-taking mouth, the animal behaviors of the experimental animal are standardized and accurately quantified, the whole experiment is enabled to have universality, and the function of detecting the temporal lobe of the corresponding brain area through the ethology experiment for search memory is achieved.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
Fig. 1 is a schematic structural diagram of a behavioural test device for animal memory search according to an embodiment of the present invention.
FIG. 2 is a diagram of the steps of a behavioral experimental method for searching memory of an animal according to an embodiment of the present invention.
Reference numerals:
1. a housing; 11. stretching the hand mouth; 12. an experiment cavity; 121. a reference chamber; 1211. a food taking port is controlled; 122. an execution cavity; 1221. executing a food taking port; 13. a food cavity; 2. contrasting the shading plate; 3. executing a shading plate; 4. a detection component; 41. a first detecting member; 42. a second detecting member; 43. a third detecting member; 5. contrasting with a turning plate; 6. executing a plate turnover; 7. a zero button.
Detailed Description
In order to make the technical problems solved, the technical solutions adopted and the technical effects achieved by the present invention clearer, the technical solutions of the present invention are further described below by way of specific embodiments with reference to the accompanying drawings.
In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the invention.
Furthermore, features defined as "second" and "third" may explicitly or implicitly include one or more of the features for distinguishing between descriptive features, non-sequential, non-trivial and non-trivial. In the description of the present invention, "a plurality" means two or more unless otherwise specified.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The following describes a specific structure of a behavioural experimental apparatus for animal memory search according to an embodiment of the present invention with reference to fig. 1.
As shown in fig. 1, the behavioural experiment device for animal search memory according to the embodiment of the present invention comprises a housing 1, a control light shielding plate 2, an executive light shielding plate 3, a detection component 4, a control turning plate 5, a support turning plate 6 and a control component (not shown).
As shown in fig. 1, an experiment cavity 12 and a food cavity 13 are separately arranged in a housing 1, the experiment cavity 12 is separately provided with a comparison cavity 121 and an execution cavity 122, a plurality of comparison food taking ports 1211 are arranged on the bottom wall of the comparison cavity 121, the plurality of comparison food taking ports 1211 are respectively communicated with the food cavity 13, a plurality of execution food taking ports 1221 are arranged on the bottom wall of the execution cavity 122, the plurality of execution food taking ports 1221 are respectively communicated with the food cavity 13, two hand stretching ports 11 are arranged on the housing 1, the two hand stretching ports 11 are respectively arranged corresponding to the comparison cavity 121 and the execution cavity 122, the comparison light shielding plate 2 is slidably arranged on the housing 1, the comparison light shielding plate 2 is used for shielding the comparison cavity 121, the execution light shielding plate 3 is slidably arranged on the housing 1, the execution light shielding plate 3 is used for shielding the execution cavity 122, and a detection component 4 is configured to detect whether the comparison light shielding plate 2 shields the comparison cavity 121 or not, Whether the execution light shielding plate 3 shields the execution cavity 122 or not is detected, whether the arms of the experimental animal penetrate through the hand stretching port 11 or not is detected, whether the arms of the experimental animal penetrate through the execution food taking port 1221 or not is detected, a plurality of comparison turning plates 5 are arranged, each comparison turning plate 5 corresponds to one comparison food taking port 1211, the comparison turning plates 5 are arranged, the color of one comparison turning plate 5 is different from that of the other comparison turning plates 5, a plurality of support turning plates 6 are arranged, each support turning plate 6 corresponds to one execution food taking port 1221, the color of the plurality of support turning plates 6 is the same, the distribution mode of the plurality of support turning plates 6 is the same as the distribution direction of the comparison turning plates 5, the control assembly comprises a timing unit, and the timing unit is configured to record first time length from the time when the comparison light shielding plate 2 is removed to the execution light shielding plate 3 is removed, record second time length from the time when the arms of the execution light shielding plate 3 are removed to the experimental animal penetrate into the hand stretching port 11, and record time length from the arms of the experimental animal penetrate into the hand stretching port 11 to the arms of the experimental animal A third duration of the food port 1221.
It can be understood that, place the laboratory animal in the casing 1 front side after the experiment begins, demolish contrast turning plate 5 and make the laboratory animal can observe contrast turning plate 5 colour in the control cavity 121, will contrast turning plate 5 back and will support turning plate 6 and demolish and make the laboratory animal can see the distribution condition that supports turning plate 6 after a certain time, because the distribution shape that supports turning plate 6 is the same with contrast turning plate 5, the laboratory animal that search for memory's ability stronger can stretch into the execution cavity 122 and stretch into the arm and get food mouthful 1221 in the execution that support turning plate 6 that the contrast turning plate 5 that is different with the colour corresponds the setting.
In the present embodiment, in practical situations, the length of time from seeing the distribution of the support flaps 6 in the execution cavity 122 to extending the hands into the hand-stretching openings 11 of the experimental animals is greatly different according to different types of the experimental animals, different domestication degrees, different characters of the experimental animals and different health states. In the embodiment, the control component can record the second time period of the arm of the laboratory animal extending into the hand extending opening 11 from the removing execution shading plate 3, so that the search memory capacity and the reaction capacity of the laboratory animal can be preliminarily reflected. Meanwhile, after the arms of the experimental animals extend into the hand stretching openings 11, some experimental animals can have a phenomenon of hesitant thinking according to the difference of the search memory capacity, in this embodiment, the control component can record a third time period from the time that the arms of the experimental animals extend into the hand stretching openings 11 to the time that the arms of the experimental animals extend into a certain execution food taking opening 1221, and the third time period can further reflect the search memory capacity of the experimental animals. Therefore, the search memory level and the execution capacity of the experimental animal can be qualitatively and quantitatively analyzed by combining the lengths of the second time length and the third time length.
In addition, the length of time for which the experimental animal observes the distribution of control flaps 5 in the control chamber during the experiment also affects the results of the whole experiment. In the invention, the control component can also record the first time length from the removal of the control shading plate 2 to the removal of the execution shading plate 3, and the first time length can reflect the observation time length of the experimental animal. Therefore, the accuracy of the experimental result is improved, and the function of the corresponding temporal lobe of the brain area of the experimental animal can be analyzed by experimenters.
According to the ethology experiment device for animal search memory, provided by the embodiment of the invention, the control component can record the first time from the removal of the control light shielding plate 2 to the removal of the execution light shielding plate 3, the second time from the removal of the execution light shielding plate 3 to the extension of the arm of the experimental animal into the hand stretching port 11 and the third time from the extension of the arm of the experimental animal into the hand stretching port 11 to the extension of the arm of the experimental animal into the food taking port 1221, so that the whole ethology experiment for search memory is divided into three time periods of observing the distribution of the control turning plate 5, extending the arm into the hand stretching port 11 and extending the arm into the food taking port 1221, the animal behaviors of the experimental animal are standardized and accurately quantified, the whole adaptive experiment is universal, and the function of detecting the temporal lobe of the corresponding brain area is realized through the device designed by detecting the memory state of the animal.
In some embodiments, the detecting assembly 4 includes a first detecting member 41, a second detecting member 42, and a third detecting member 43. The number of the first detecting pieces 41 is two, the two first detecting pieces 41 are respectively arranged on the outer side walls of the comparison cavity 121 and the execution cavity 122, one of the first detecting pieces 41 is configured to detect whether the execution shading plate 3 blocks the execution cavity 122, the other one of the first detecting pieces 41 is configured to detect whether the comparison shading plate 2 blocks the comparison cavity 121, the number of the second detecting pieces 42 is two, each second detecting piece 42 is arranged on the inner wall of one hand extending port 11, the second detecting pieces 42 are configured to detect whether the arm of the experimental animal passes through the hand extending port 11, the number of the third detecting pieces 43 is multiple, each third detecting piece 43 is arranged on the inner wall of one execution food taking port 1221, and the third detecting pieces 43 are configured to detect whether the arm of the experimental animal passes through the execution food taking port 1221.
It can be understood that the first detecting part 41 can accurately determine the time point for removing the control light shielding plate 2 and the executing light shielding plate 3, and the timing unit can accurately record the first time length from the removal of the control light shielding plate 2 to the removal of the executing light shielding plate 3, thereby ensuring the accuracy of the experimental result. The second detection piece 42 can accurately determine the time point when the arm of the experimental animal passes through the hand stretching opening 11, and the cooperation of the first detection piece 41 and the second detection piece 42 ensures that the timing unit can accurately remove the execution light shielding plate 3 until the second time when the arm of the experimental animal stretches into the hand stretching opening 11, so that an experimenter can more accurately analyze the reaction capability of the experimental animal and preliminarily judge the search memory capability of the experimental animal. The third detection piece 43 can accurately judge which execution food taking port 1221 the arm of the experimental animal stretches into, and accurately determine the accurate time point when the arm of the experimental animal stretches into the execution food taking port 1221, and the second detection piece 42 and the third detection piece 43 are used in cooperation, so that the timing unit can accurately record the third time from the time when the arm of the experimental animal stretches into the hand stretching port 11 to the time when the arm of the experimental animal stretches into the execution food taking port 1221, thereby not only being convenient for an experimenter to judge whether the experimental animal successfully takes out food from the correct execution food taking port 1221, but also being convenient for the experimenter to analyze and judge the level of search memory of the experimental animal according to the third time.
Advantageously, the first detecting member 41 is a light sensor, and the second and third detecting members 42, 43 are touch sensors.
Advantageously, a third detecting member 43 may also be provided at each control access 1211. Thus, after adjusting the color distribution of the control mask 2 and the implement mask 3, the implement cavity 122 can be used as the control cavity 121, and the control cavity 121 can be used as the implement cavity 122 for experiments.
It should be added here that the control logic between the first, second and third detecting members 41, 42, 43 and the timing assembly of the control assembly should be selectable by those skilled in the art according to the prior art, and no more than the control logic between the first, second and third detecting members 41, 42, 43 and the timing assembly of the control assembly and the selection of the relevant circuit design and electrical components are specifically limited here.
In some specific embodiments, the timing unit includes a first timer, a second timer and a third timer, the first timer records a first time period from the time when one first detecting element 41 detects that the control light shielding plate 2 is separated from the control cavity 121 to the time when another first detecting element 41 detects that the execution light shielding plate 3 is separated from the execution cavity 122 as a first time period, the second timer records a second time period from the time when the first detecting element 41 detects that the execution light shielding plate 3 is separated from the execution cavity 122 to the time when the second detecting element 42 detects that the arm of the experimental animal passes through the hand stretching opening 11, and the third timer records a third time period from the time when the second detecting element 42 detects that the arm of the experimental animal passes through the hand stretching opening 11 to the time when the third detecting element 43 detects that the arm of the experimental animal passes through the execution food taking opening 1221. It can be understood that the first time length, the second time length and the third time length can be accurately recorded through the first timer, the second timer and the third timer, so that the recording of experimental data by experimenters and the qualitative and quantitative analysis of the search memory level of experimental animals are facilitated, and the accuracy and the representativeness of experimental results are ensured.
Here, the first timer may be two physical timing devices electrically connected to the first detecting element 41, or may be a logic program for timing of a control logic of the control assembly, the second timer may be a physical timing device electrically connected to the first detecting element 41 and the second detecting element 42, or may be a logic program for timing of a control logic of the control assembly, and the third timer may be a physical timing device electrically connected to the second detecting element 42 and the third detecting element 43, or may be a logic program for timing of a control logic of the control assembly, which may be specifically selected according to actual needs.
In some specific embodiments, the control assembly comprises a zero button 7, and the zero button 7 is electrically connected with the first timer, the second timer and the third timer. It is understood that in the actual experiment process, the experiment may be performed on the same experimental animal for a plurality of times, or on a plurality of different experimental animals. The zero setting button 7 can realize zero setting of the first timer, the second timer and the point timer after one experiment is finished, and the next experiment is convenient to carry out. It should be noted that, in this embodiment, the zero button 7 may be an entity button electrically connected to the first timer, the second timer, and the third timer, or may be a logic program used by the control component to zero, and may be specifically selected according to actual needs.
In some optional embodiments, the detecting assembly 4 further includes a plurality of indicator lights, the plurality of indicator lights are disposed in one-to-one correspondence with the plurality of third detecting members 43, and each indicator light is electrically connected to the third detecting member 43. From this, the pilot lamp can send the flash of light after the experimental animals 'arm passes the execution and gets food mouth 1221 to further made things convenient for the experimenter to observe experimental animals's execution result, made things convenient for the experimenter to carry out the experiment record.
In some alternative embodiments, the control food intake 1211 and the execute food intake 1221 are arranged in rows and columns. It can be understood that the more regular arrangement form can reflect the general level of the experimental animals, and the phenomenon that the experimental animals can be successfully operated and a plurality of experimental animals fail to operate in a plurality of experiments is avoided. After the preliminary experiment, the arrangement mode of the control food taking opening 1211 and the food taking openings can be adjusted, so that the next experiment can be performed in a targeted manner, and animals with strong search memory capacity, ordinary search memory capacity and weak search memory capacity can be well distinguished.
Example (b):
the following describes a specific structure of a behavioural test device for animal memory search according to a specific embodiment of the present invention with reference to fig. 1.
As shown in fig. 1, the behavioral experiment apparatus for animal search memory according to the embodiment of the present invention includes a housing 1, a control light shielding plate 2, an execution light shielding plate 3, a detection component 4, a control turning plate 5, a support turning plate 6, and a control component.
The shell 1 is internally provided with an experiment cavity 12 and a food cavity 13 in a separated manner, the experiment cavity 12 is provided with a control cavity 121 and an execution cavity 122 in a separated manner, the bottom wall of the control cavity 121 is provided with nine control food taking ports 1211, the nine control food taking ports 1211 are respectively communicated with the food cavity 13, and the nine control food taking ports 1211 are arranged in three rows and three columns. Nine executive food taking ports 1221 are formed in the bottom wall of the executive cavity 122, the nine executive food taking ports 1221 are respectively communicated with the food cavity 13, and the nine executive food taking ports 1221 are arranged in three rows and three columns.
Be equipped with two on the casing 1 and stretch out hand mouth 11, two stretch out hand mouth 11 and correspond contrast cavity 121 and execution cavity 122 setting respectively, establish on casing 1 contrast light screen 2 slidable, contrast light screen 2 is used for sheltering from contrast cavity 121, and execution light screen 3 slidable is established on casing 1, and execution light screen 3 is used for sheltering from execution cavity 122.
The detecting assembly 4 includes a first detecting member 41, a second detecting member 42, and a third detecting member 43. The number of the first detecting pieces 41 is two, the two first detecting pieces 41 are respectively arranged on the outer side walls of the comparison cavity 121 and the execution cavity 122, one first detecting piece 41 is configured to detect whether the execution shading plate 3 blocks the execution cavity 122, the other first detecting piece 41 is configured to detect whether the comparison shading plate 2 blocks the comparison cavity 121, the number of the second detecting pieces 42 is two, each second detecting piece 42 is arranged on the inner wall of one hand extending port 11, the second detecting pieces 42 are configured to detect whether the arm of the experimental animal passes through the hand extending port 11, the number of the third detecting pieces 43 is nine, each third detecting piece 43 is arranged on the inner wall of one execution food taking port 1221, and the third detecting pieces 43 are configured to detect whether the arm of the experimental animal passes through the execution food taking port 1221. Nine comparison turning plates 5 are provided, each comparison turning plate 5 is arranged corresponding to one comparison food taking opening 1211, and the nine comparison turning plates 5 are provided, wherein one comparison turning plate 5 is different from the other comparison turning plates 5 in color, nine support turning plates 6 are provided, each support turning plate 6 is arranged corresponding to one execution food taking opening 1221, the nine support turning plates 6 are same in color, the nine support turning plates 6 are same in distribution direction with the comparison turning plates 5, the control component comprises a timing unit, the timing unit comprises a first timer, a second timer and a third timer, the first timer records that the first time length is the time length when one first detection piece 41 detects that the comparison light shielding plate 2 is separated from the comparison cavity 121 to when the other first detection piece 41 detects that the execution light shielding plate 3 is separated from the execution cavity 122, and the second time length is the time length when the first detection piece 41 detects that the execution light shielding plate 3 is separated from the execution cavity 122 to when the second detection piece 42 detects that the arm of the experimental animal passes through the hand taking opening 11 The length is a second length, and the third timer records the length from the time when the second detecting part 42 detects that the arm of the experimental animal passes through the hand stretching opening 11 to the time when the third detecting part 43 detects that the arm of the experimental animal passes through the feeding execution opening 1221 as the second length.
The experimental procedure of the behavioristic experimental apparatus for animal memory search of this example is as follows: the experimental animal is placed in front of the shell 1, the first timer starts to time after the control shading plate 2 is detached, the execution shading plate 3 is detached after a period of time, the control shading plate 2 is installed back, the first timer stops timing and records the first time length recorded by the first timer, the second timer starts to time, and if the time recorded by the second timer reaches the first preset time length, the second detection piece 42 still does not detect that the arm of the experimental animal stops in the experiment. If the second detection piece 42 detects that the arm of the experimental animal passes through the hand stretching opening 11 within the first preset time period, the second timer stops timing, and the third timer starts timing. If the third detector 43 does not detect that the arm of the experimental animal passes through the feeding port 1221 after the third timer records that the time reaches the second preset time period, the experiment is stopped. If the test animal removes food from a certain performed food intake 1221 within a second preset length of time, a record is made and the test is terminated.
The following describes specific steps of a behavioral experimental method for searching memory of an animal according to an embodiment of the present invention with reference to fig. 2.
The animal search memory behavioristics experimental method provided by the embodiment of the invention is carried out by adopting the animal search memory behavioristics experimental device, as shown in fig. 2, and comprises the following steps;
s1: the contrast shading plate 2 is removed;
s2: removing the executing light shielding plate 3, and recording a first time length from the removal of the reference light shielding plate 2 to the removal of the executing light shielding plate 3;
s3: recording a second time from the dismantling of the executing shading plate 3 to the time when the arm of the experimental animal extends into the hand stretching opening 11;
s4: recording a third time period from the time the arm of the experimental animal extends into the hand-extending opening 11 to the time the arm of the experimental animal extends into the feeding execution opening 1221.
It can be understood that, in the course of the behavioural experiment, the first time period from the removal of the control light shielding plate 2 to the removal of the execution light shielding plate 3, the second time period from the removal of the execution light shielding plate 3 to the extension of the arm of the experimental animal into the hand stretching port 11 and the third time period from the extension of the arm of the experimental animal into the hand stretching port 11 to the extension of the arm of the experimental animal into the food taking port 1221 are recorded, the whole behavioural experiment for search and memory is divided into three time periods of observation of the distribution of the control turning plate 5, the extension of the arm into the hand stretching port 11 and the extension of the arm into the food taking port 1221, so that the animal behaviour standardization and the accurate quantification of the experimental animal are realized, the whole experiment has universality, and the function of detecting the corresponding temporal lobe of the brain area through the behavioural experiment for search and memory is achieved.
In some alternative embodiments, in step S2, the control shield 2 is installed while the execution shield 3 is removed so that the shield occludes the control chamber 121. It can be understood that, demolish execution light screen 3, control light screen 2 still keeps demolishd the state, and the condition in control cavity 121 still can be observed to the laboratory animal this moment, may produce harmful effects to the experiment like this, in this embodiment demolish execution light screen 3 the time install control light screen 2 so that the light screen shelters from control cavity 121, guaranteed like this to demolish the unable circumstances of observing in the control cavity 121 of laboratory animal behind the execution light screen 3 to the accuracy of experimental result has been guaranteed.
In some alternative embodiments, in step S3, after the light shielding plate 3 is removed and executed for the first preset time, the arm of the experimental animal still does not pass through the hand stretching opening 11, and the experiment is stopped; in step S4, after the arm of the experimental animal passes through the hand stretching opening 11 for a second preset time, the arm of the experimental animal does not extend into the feeding execution opening 1221, and the experiment is stopped.
It can be understood that, in the experiment process, the experimental animal can accurately take out food from the corresponding execution food taking port 1221 in the execution box body, and the execution of the experimental animal is calibrated to be successful; if the experimental animal extends the arm into the wrong execution feeding port 1221, the calibration animal fails to execute; and if the experimental animal does not carry out the feeding operation after the executing shading plate 3 is detached for a period of time, the non-executing behavior of the experimental animal is calibrated. However, if the experimental animal is always waiting for the completion of the food taking operation, the experimental animal may not extend the arm into the hand stretching opening 11 in some cases due to the difference of the auditory association matching ability and the execution ability of the experimental animal, and much time is wasted if the experiment is not stopped. In the embodiment of the invention, the first preset time and the second preset time are set as the nodes for forcibly stopping the experiment, so that the experiment time is saved, and the experiment efficiency is improved.
In the description herein, references to the description of "some embodiments," "other embodiments," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The above description is only a preferred embodiment of the present invention, and for those skilled in the art, the present invention should not be limited by the description of the present invention, which should be interpreted as a limitation.
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| CN110839558B (en) * | 2019-11-19 | 2021-07-06 | 中国科学院深圳先进技术研究院 | A behavioral experimental device and experimental method for animal auditory association matching |
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