CN110833047B - A behavioral experimental device and experimental method for animal visuospatial cognitive memory - Google Patents
A behavioral experimental device and experimental method for animal visuospatial cognitive memory Download PDFInfo
- Publication number
- CN110833047B CN110833047B CN201911134341.7A CN201911134341A CN110833047B CN 110833047 B CN110833047 B CN 110833047B CN 201911134341 A CN201911134341 A CN 201911134341A CN 110833047 B CN110833047 B CN 110833047B
- Authority
- CN
- China
- Prior art keywords
- experimental
- animal
- lever
- behavioral
- experiment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K29/00—Other apparatus for animal husbandry
- A01K29/005—Monitoring or measuring activity, e.g. detecting heat or mating
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Biophysics (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Eye Examination Apparatus (AREA)
Abstract
本发明公开了一种动物视空间认知记忆的行为学实验装置及实验方法，该行为学实验装置包括壳体、遮光板、检测组件、实验杠杆和翻板。壳体内限定出分隔开的实验腔体和食物腔体，壳体的侧壁上设有与实验腔体连通的伸手口，实验腔体的底壁上设有与食物腔体连通的取食口，遮光板可滑动地设在壳体上，遮光板配合在设有伸手口的壳体的侧壁上，检测组件包括计时单元，计时单元被配置为检测遮光板是否封闭伸手口以及检测实验动物的手臂是否穿过伸手口，实验杠杆上套设有握持件，翻板可转动的配合在取食口处。该实验装置的实验难度相对较低，且能够较好地量化实验动物的认知水平。
The invention discloses a behavioral experimental device and an experimental method for animal visual space cognition and memory. The behavioral experimental device comprises a casing, a shading plate, a detection component, an experimental lever and a flap. The shell defines a separate experimental cavity and a food cavity, the side wall of the shell is provided with a hand opening that communicates with the experimental cavity, and the bottom wall of the experimental cavity is provided with a food intake that communicates with the food cavity The shading plate is slidably arranged on the casing, the shading plate is fitted on the side wall of the casing with the hand-reaching mouth, the detection component includes a timing unit, and the timing unit is configured to detect whether the shading plate closes the hand-reaching mouth and the detection experiment Whether the animal's arm passes through the hand opening, the experimental lever is sleeved with a grip, and the flap is rotatably fitted at the feeding opening. The experimental difficulty of the experimental device is relatively low, and it can better quantify the cognitive level of experimental animals.
Description
Technical Field
The invention relates to the technical field of animal behavioural research, in particular to a behavioural experimental device and an experimental method for cognitive memory of an animal visual space.
Background
With the development of scientific technology, animals have been widely used in medical biology (including behavioural) testing and research due to great resemblance in neuroanatomy, neurophysiology, immunology and genetic characteristics to humans. Behavioral training is the study of clinical characteristics of animals, and animals complete target tasks in training to achieve the purpose of evaluating certain indexes so as to reveal the essence and the law of life activities, health and diseases. However, it is difficult and complicated to deploy a reasonable behavioral apparatus because it is a large, smart, social, long-lived, non-domesticated, and offensive animal. The previous research on cognitive memory of each visual space focuses on the test of working memory.
The prior art is directly putting food on the vertical rod, and through the difficulty degree of setting the rod, the time of taking food by the animal is evaluated, and the like. The requirement of this device to food is comparatively strict, and food is generally all more easily crumbled, has caused the experiment degree of difficulty, and current experimental apparatus can not carry out the quantitative judgement to experimental animals's visual space cognitive memory level.
Disclosure of Invention
The invention aims to provide a behavioristic experimental device for cognitive memory of an animal visual space, which has relatively low experimental difficulty and can better quantify the cognitive level of an experimental animal.
The invention also aims to provide a behavioral experimental method for the cognitive memory of the animal visual space, which can better quantify the cognitive level of the experimental animal.
In order to achieve the technical effects, the technical scheme of the invention is as follows:
a behavioristics experimental device for cognitive memory of animal visual space comprises: the device comprises a shell, an experiment cavity and a food cavity are arranged in the shell in a separated mode, a hand stretching port communicated with the experiment cavity is formed in the side wall of the shell, and a food taking port communicated with the food cavity is formed in the bottom wall of the experiment cavity; the shading plate is arranged on the shell in a sliding mode and matched with the side wall of the shell provided with the hand stretching opening; the experimental device comprises an experimental lever, a clamping piece is sleeved on the experimental lever, and an experimental animal can clamp the clamping piece to slide relative to the experimental lever; a detection component configured to detect whether the light shielding plate closes the hand-stretching opening, detect whether an arm of the experimental animal passes through the hand-stretching opening, and detect whether the holding piece reaches a specified position on the experimental lever; a control assembly including a timing unit configured to record a first length of time to remove the shutter plate to pass an arm of the laboratory animal through the hand-reaching opening and a second length of time to slide the grip from the first end of the laboratory lever to the second end of the laboratory lever; a flap rotatably disposed at the access opening, the flap configured to rotate to open the access opening when the detection assembly detects that the grip is moved to the second end.
In some embodiments, the control assembly comprises: the first detection piece is arranged on the side wall of the shell, which is matched with the shading plate, and is configured to detect whether the shading plate seals the hand stretching opening or not; the second detection piece is arranged on the inner wall of the hand stretching opening and is configured to detect whether the arm of the experimental animal passes through the hand stretching opening or not; and the number of the third detection pieces is two, and the two third detection pieces are respectively arranged at the first end and the second end of the experiment lever.
In some embodiments, the timing unit comprises: the first timer records that the first time length from the time when the first detection piece detects that the light shielding plate is separated from the side wall of the shell to the time when the second detection piece detects that the arm of the experimental animal is the first time length;
and the second timer records that the time length from the time when one third detection piece detects the holding piece to the time when the other third detection piece detects the holding piece is the second time length.
In some embodiments, the behavioral experimental apparatus for cognitive memory in animal visual space further comprises: the magnetic attraction structure is characterized in that one part of the magnetic attraction structure is connected with the turning plate, the other part of the magnetic attraction structure is connected with the bottom wall of the shell, the magnetic attraction structure is electrically connected with the control component, and the two parts of the magnetic attraction structure are controlled by the control component to attract or not; and one end of the elastic piece is connected with the turning plate, and the other end of the elastic piece is connected with the bottom wall of the shell.
In some embodiments, the experimental lever is a linear lever.
In some specific embodiments, the linear lever comprises: two linear portions; and the two ends of the protruding part are respectively connected with the two linear parts.
In some more specific embodiments, the convex portion includes at least one square-shaped protrusion protruding in a direction perpendicular to an extending direction of the linear portion.
In some embodiments, a partition board is arranged in the shell, the partition board divides the experiment cavity into two sub-cavities, each sub-cavity is internally provided with one experiment lever, each sub-cavity is provided with one hand stretching opening, each sub-cavity is provided with the light shielding plate matched with the side wall of the hand stretching opening, and the bottom wall of each sub-cavity is provided with the food taking opening communicated with the food cavity.
A behavioristics experimental method for animal visual space cognition memory is carried out by adopting the behavioristics experimental device for animal visual space cognition memory, and comprises the following steps;
s1: removing the light shielding plate fitted on the side wall of the housing;
s2: recording the first time length for detaching the light shielding plate until the arm of the experimental animal passes through the hand stretching opening;
s3: the grip slides from the first end of the experiment lever to the second end of the experiment lever for the second length of time.
In some embodiments, in step S2, after the light shielding plate is removed for a first preset time, the arm of the experimental animal still does not pass through the hand stretching opening, and the experiment is stopped;
in step S3, after the arm of the experimental animal passes through the hand stretching opening for a second preset time, the grip does not move from the first end to the second end of the experimental lever, and the experiment is stopped.
According to the behavioral experiment device for the visual space cognitive memory of the animal, disclosed by the embodiment of the invention, the control component can record the first time length from the removal of the light shielding plate to the passing of the arm of the experimental animal through the hand stretching port and the second time length from the sliding of the holding piece from the first end of the experimental lever to the second end of the experimental lever, and the behavioral experiment for the visual space cognitive memory is divided into two time periods, so that the behavior of the experimental animal is subjected to standardized division and quantitative judgment, the visual space memory of the animal is further evaluated, and the function of detecting the corresponding top lobe of the brain region is realized. In addition, in the actual experiment process, the holding piece can slide relative to the experiment lever to carry out the experiment, so that the phenomenon that the holding piece is crushed by the animal is avoided, and the success rate of the experiment is ensured.
According to the behavioral experiment method for the animal visual space cognitive memory, in the experiment process, the first time length from the removal of the light shielding plate to the passing of the arm of the experimental animal through the hand stretching port and the second time length from the sliding of the holding piece from the first end of the experimental lever to the second end of the experimental lever can be recorded, and the behavioral experiment for the visual space cognitive memory is divided into two time periods, so that the behavior of the experimental animal is subjected to standardized division and quantitative judgment, the visual-spatial memory of the animal is further evaluated, and the function of detecting the corresponding top lobe of the brain region is realized.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
Fig. 1 is a schematic structural diagram of a behavioural experimental device for animal visual space cognitive memory according to an embodiment of the present invention.
Fig. 2 is a schematic structural diagram of an experimental lever according to an embodiment of the present invention.
FIG. 3 is a schematic structural diagram of another experimental lever provided in the embodiment of the present invention.
Reference numerals:
1. a housing; 11. an experiment cavity; 12. a food cavity; 13. stretching the hand mouth; 14. a food taking port;
2. a visor;
3. a detection component; 31. a first detecting member; 32. a second detecting member; 33. a third detecting member;
4. an experiment lever; 41. a straight portion; 42; a boss portion;
5. a grip;
6. turning over a plate;
7. a partition plate;
8. a reset button.
Detailed Description
In order to make the technical problems solved, the technical solutions adopted and the technical effects achieved by the present invention clearer, the technical solutions of the present invention are further described below by way of specific embodiments with reference to the accompanying drawings.
In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the invention.
Furthermore, features defined as "second" and "third" may explicitly or implicitly include one or more of the features for distinguishing between descriptive features, non-sequential, non-trivial and non-trivial. In the description of the present invention, "a plurality" means two or more unless otherwise specified.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The following describes a specific structure of a behavioural experimental device for animal visuospatial cognitive memory according to an embodiment of the present invention with reference to fig. 1 to 3.
As shown in fig. 1, the behavioural test device for cognitive memory of animal visual space according to the embodiment of the present invention comprises a housing 1, a light shielding plate 2, a detection component 3, a test lever 4, a control component (not shown in the figure) and a turning plate 6.
As shown in fig. 1, separate in the casing 1 and be provided with experiment cavity 11 and food cavity 12, be equipped with on the lateral wall of casing 1 with the mouth 13 of stretching one's hand of experiment cavity 11 intercommunication, be equipped with on the diapire of experiment cavity 11 and get food mouth 14 with food cavity 12 intercommunication, establish on casing 1 light screen 2 slidable, light screen 2 cooperates on the lateral wall of casing 1 that is equipped with the mouth 13 of stretching one's hand, the cover is equipped with gripping member 5 on the experiment lever 4, experimental animals can grip 5 and slide relative experiment lever 4 of gripping member 5, detection component 3 is configured as and detects whether light screen 2 seals the mouth 13 of stretching one's hand, whether the arm of detecting experimental animals passes the mouth 13 of stretching one's hand and detects whether the gripping member 5 reachs the assigned position on the experiment lever 4. The control assembly comprises a timing unit which is configured to record a first time length from the light shielding plate 2 to the arm of the experimental animal to pass through the hand stretching opening 13 and a second time length from the first end of the experimental lever 4 to the second end of the experimental lever 4, the turning plate 6 is rotatably arranged at the food taking opening 14, and the turning plate 6 is configured to rotate to open the food taking opening 14 when the detection assembly detects that the holding member 5 moves to the second end of the experimental lever 4.
It will be appreciated that the flap 6 is closed to close the access opening 14 prior to commencement of the experiment, and the visor 2 is mounted to the side wall of the housing 1 provided with the snout 13 and ensures that the experimental animal cannot see into the experimental chamber 11 from the side wall provided with the snout 13.
After the experiment preparation, place the laboratory animal in the place ahead that corresponds the lateral wall that is equipped with casing 1 of stretching hand mouth 13, remove light screen 2, wait for the laboratory animal to stretch into the arm and stretch into hand mouth 13 in and the control assembly record demolish light screen 2 to the first time length that the arm of laboratory animal passed stretch hand mouth 13, in actual conditions, according to the species difference of laboratory animal, the difference of domestication degree, the personality difference and the difference of health state of laboratory animal itself, the laboratory animal has great difference from seeing the time length of experiment lever 4 to stretching into hand mouth 13 with the hand. In this embodiment, the control module can note first duration, removes duration that the arm of light screen 2 to the laboratory animal passed the mouth of a hand 13 promptly, can tentatively analyze the cognitive ability of laboratory animal according to the length of first duration to judge whether there is cognitive disorder in the experiment.
In practical situations, according to different visual space cognition levels of the experimental animals, the lengths of time for the experimental animals to slide the holding piece 5 from the first end to the second end of the experimental lever 4 along the extending direction of the experimental lever 4 are also greatly different, and when the experimental animals extend arms into the experimental cavity 11 to hold the holding piece 5 and slide the holding piece 5 from the first end to the second end of the experimental lever 4 along the extending direction of the experimental lever 4, the behavior of the experimental animals can be standardized and quantified through data analysis of the first length of time and the second length of time according to the second length of time recorded by the control component, namely the length of time for the holding piece 5 to slide from the first end to the second end of the experimental lever 4, so that the visual space memory of the animals is evaluated, and the function of detecting corresponding brain parietal lobes is realized.
In addition, in the experiment process of the embodiment, the experimental animal is held by the holding piece 5 for experiment, and the flap 6 can be opened when the holding piece 5 is arranged at the second end of the experiment lever 4, so that the experimental animal obtains reward. In whole experimentation, the experimental animal does not directly hold food, has avoided the experimental animal to knead the food and has leaded to the phenomenon of experiment failure to take place, adopts the success rate of promotion experiment that the structure of this embodiment can be better.
According to the behavioural experiment device for the cognitive memory of the visual space of the animal, disclosed by the embodiment of the invention, the control component can record the first time length from the detachment of the light shielding plate 2 to the passing of the arm of the experimental animal through the hand stretching port 13 and the second time length from the sliding of the holding piece 5 from the first end of the experimental lever 4 to the second end of the experimental lever 4, and divide the behavioural experiment for the cognitive memory of the visual space into two time periods, so that the behavioural experiment of the cognitive memory of the visual space is divided and quantitatively judged in a standardized way, the memory of the visual space of the experimental animal is further evaluated, and the function of detecting the corresponding apical lobe of the brain region is realized. In addition, in the actual experiment process, the holding piece 5 which can slide relative to the experiment lever 4 is adopted for carrying out the experiment, the phenomenon that the holding piece 5 is crushed by the animal is avoided, and the success rate of the experiment is ensured.
It should be added that, in an actual experiment, a first preset time and a second preset time may be preset, and after the first preset time passes after the light shielding plate 2 is removed, the experimental animal still does not extend the arm into the hand stretching opening 13, and it is determined that the experimental animal does not perform a relevant action, at this time, the experiment is stopped and recorded. After the arm of the experimental animal extends into the hand extending opening 13 and holds the holding piece 5 for a second preset time, the experimental animal still does not slide the holding piece 5 to the second end of the experimental lever 4, and the experimental animal is determined to have failed to execute, and the experiment is stopped and recorded at the moment.
In some embodiments, the side wall of the housing 1 provided with the hand-stretching opening 13 is provided with two sliding grooves arranged at intervals, and two opposite sides of the light shielding plate 2 are matched in the sliding grooves. Therefore, the shading plate 2 is convenient for operators to mount or dismount. Of course, in other embodiments of the present invention, the light shielding plate 2 may also be connected to the housing 1 by a screw, a rivet, or other connecting members.
In some embodiments, as shown in fig. 1, the detecting assembly 3 includes a first detecting member 31, a second detecting member 32 and a third detecting member 33, the first detecting member 31 is disposed on a side wall of the housing 1 where the light shielding plate 2 is fitted, the first detecting member 31 is configured to detect whether the light shielding plate 2 closes the hand-stretching opening 13, the second detecting member 32 is disposed on an inner wall of the hand-stretching opening 13, the second detecting member 32 is configured to detect whether an arm of the laboratory animal passes through the hand-stretching opening 13, the number of the third detecting members 33 is two, and the two third detecting members 33 are disposed at the first end and the second end of the laboratory lever 4, respectively. It can be understood that, through setting up first detection piece 31 and second detection piece 32, can confirm the time point that light screen 2 demolishs and the time point that the arm of laboratory animal stretches into hand mouth 13 accurately, make the first time length of control assembly record more accurate like this to make the experimenter carry out more accurate quantitative judgement to the cognitive level of laboratory animal. And the movement starting point and the movement finishing point of the holding part 5 can be accurately determined by arranging the two third detection parts 33, so that the second time recorded by the control assembly is more accurate, and the experimenter can quantitatively judge the visual space memory of the experimental animal more accurately.
In some embodiments, the first detecting member 31 is a light sensor, and the second detecting member 32 and the third detecting member 33 are touch sensors. From this, whether realization that can be convenient detects light screen 2 and seals out stretching hand mouth 13, whether the arm that detects the experimental animal passes stretching hand mouth 13 and whether the function that detects the second end that grips 5 and reach experiment lever 4. Of course, in other embodiments of the present invention, the specific types of the first detecting member 31, the second detecting member 32 and the third detecting member 33 can be selected according to actual needs, and are not limited to the structures of the light sensor and the touch sensor of the present embodiment.
In some embodiments, the timing unit includes a first timer that records a first period of time from when the first detecting member 31 detects that the light shielding plate 2 is separated from the side wall of the housing 1 to when the second detecting member 32 detects the arm of the laboratory animal, and a second timer that records a second period of time from when one third detecting member 33 detects the grip 5 to when the other third detecting member 33 detects the grip 5. It can be understood that the first time length and the second time length can be accurately recorded through the first timer and the second timer, so that the experimental data can be conveniently recorded by an experimenter, and the cognitive ability and the visual space memory level of the experimental animal can be conveniently judged. Here, the first timer may be a physical timing device electrically connected to the first detecting element 31 and the second detecting element 32, or may be a logic program for timing of control logic of the control assembly, and the second timer may be a physical timing device electrically connected to the two third detecting elements 33, or may be a logic program for timing of control logic of the control assembly, and may be specifically selected according to actual needs.
In some embodiments, the behavioural experiment device for cognitive memory of the animal visual space further comprises a magnetic attraction structure and an elastic member, wherein one part of the magnetic attraction structure is connected with the turning plate 6, the other part of the magnetic attraction structure is connected with the bottom wall of the shell 1, the magnetic attraction structure is electrically connected with the control component, the two parts of the magnetic attraction structure are controlled by the control component to attract or not, one end of the elastic member is connected with the turning plate 6, and the other end of the elastic member is connected with the bottom wall of the shell 1. Therefore, in the process that the holding piece 5 moves from the first end to the second end of the experiment lever 4, the turning plate 6 cannot be opened, namely, the experiment animal cannot be rewarded, only when the holding piece 5 reaches the second end of the experiment lever 4, the turning plate 6 can be opened, and at the moment, the experiment animal can obtain the reward. Thus, the phenomenon that the turnover plate 6 is opened when the grip is positioned at the middle position of the experiment lever 4 to cause an accident in the experiment is avoided.
Advantageously, the behavioristics experimental apparatus for cognitive memory of the animal visual space further comprises a reset button 8, and the reset button 8 is used for resetting the turning plate 6, so that the user can conveniently reset the turning plate 6.
In some embodiments, the experiment lever 4 is a linear lever. From this, experiment lever 4 sets up to comparatively simple straight line lever can realize the differentiation to sick animal, for example the animal that has cognitive disorder. In particular, if the structure of the experiment lever 4 is complicated, most of the experiment animals can not complete the task, which may result in wrong judgment result.
In some specific embodiments, as shown in fig. 2, the linear lever includes two linear portions 41 and two protruding portions 42, and two ends of the protruding portion 42 are respectively connected to the two linear portions 41. It will be appreciated that after the simpler experimental lever 4 is used to distinguish between normal and diseased animals, further distinction between normal animals is required, and the use of a linear lever with a boss 42 can better distinguish between the visual space cognitive memory levels of different experimental animals.
In some more specific embodiments, as shown in fig. 2, the convex portion 42 includes at least one square-shaped protrusion protruding in a direction perpendicular to the extending direction of the linear portion 41. Therefore, the visual space cognitive memory levels of different experimental animals can be further distinguished.
It should be added that, in the embodiment of the present invention, the experiment lever 4 can be selected to have different structures according to the cognitive level of the actual experiment animal and the difference of the cognitive memory level of the visual space, and is not limited to the above structure.
In some embodiments, as shown in fig. 1, a partition plate 7 is provided in the housing 1, the partition plate 7 divides the experiment cavity 11 into two sub-cavities, an experiment lever 4 is provided in each sub-cavity, each sub-cavity has a hand stretching port 13, a light shielding plate 2 is provided on a side wall of each sub-cavity provided with the hand stretching port 13, and a food taking port 14 communicated with the food cavity 12 is provided on a bottom wall of each sub-cavity. It can be understood that in the actual operation process, the sick animals may have symptoms such as hemiplegia, and the execution efficiency of the same animal adopting the left hand and the right hand is also different, in the embodiment of the invention, the experiment cavity 11 is divided into two sub cavities, so that the experiment animal can be removed by the light screen 2 on the left side to perform the left-hand experiment, and the experiment animal can be removed by the light screen 2 on the right side to perform the right-hand experiment. Therefore, the strength of the execution capacity of the left hand and the right hand of the same animal can be judged, a special experiment of a sick animal can be carried out, and the application range of the animal visual space cognition and behavior experiment device is expanded.
Example (b):
the following describes a specific structure of a behavioural experimental device for animal visuospatial cognitive memory according to a specific embodiment of the present invention with reference to fig. 1.
The behavioristic experiment device for cognitive memory of the animal visual space comprises a shell 1, a light shielding plate 2, a detection assembly 3, an experiment lever 4, a turning plate 6 and a partition plate 7.
The separation is provided with experiment cavity 11 and food cavity 12 in the casing 1, and baffle 7 is established in experiment cavity 11 in order to cut apart into two sub-cavities with experiment cavity 11, be equipped with respectively on casing 1's the lateral wall with two sub-cavity intercommunication stretch hand mouth 13, all be equipped with on the diapire of two sub-cavities with food cavity 12 intercommunication get edible mouthful 14, every all be equipped with experiment lever 4 and detection component 3 in the sub-cavity. The light screen 2 is two, and two light screens 2 correspond two sub cavities and set up. The cover is equipped with the holder 5 on the experiment lever 4, laboratory animal can hold the relative experiment lever 4 slip of holder 5, every determine module 3 includes first determine part 31, second determine part 32 and third determine part 33, first determine part 31 is established on the cooperation of casing 1 has the lateral wall of light screen 2, first determine part is disposed and is detected whether light screen 2 seals and stretches hand mouth 13, second determine part 32 is established on the inner wall of stretching hand mouth 13, second determine part 32 is disposed and is detected laboratory animal's arm and whether pass and stretch hand mouth 13, third determine part 33 is two, two third determine part 33 establish respectively at the first end and the second end of experiment lever 4. The control assembly comprises a first timer and a second timer, the first timer records that the time length from the first detection piece 31 detecting that the light shielding plate 2 is separated from the side wall of the housing 1 to the second detection piece 32 detecting the arm of the experimental animal is a first time length, and the second timer records that the time length from one third detection piece 33 detecting the holding piece 5 to the other third detection piece 33 detecting the holding piece 5 is a second time length. A flap 6 is rotatably fitted at the access opening 14, the flap 6 being configured such that when the detection means detect that the grip 5 is from the second end of the test lever 4, the flap 6 rotates to open the access opening 14.
A behavioral experiment method for cognitive memory of animal visual space is carried out by adopting the behavioral experiment device for cognitive memory of animal visual space, and comprises the following steps;
s1: the shading plate 2 is detachably matched on the side wall of the shell 1;
s2: recording a first time length from the removal of the light screen 2 to the passing of the arm of the experimental animal through the hand stretching opening 13;
s3: a second length of time for the grip 5 to slide from the first end of the test lever 4 to the second end of said test lever 4 is recorded.
It can be understood that the behavioural experiment method for cognitive memory of the animal visual space provided by the embodiment of the invention can record the first time length from the removal of the light shielding plate 2 to the passing of the arm of the experimental animal through the hand stretching port 13 and the second time length from the sliding of the holding piece 5 from the first end of the experimental lever 4 to the second end of the experimental lever 4, and divide the behavioural experiment for cognitive memory of the visual space into two time periods, so that the behavioural experiment of the experimental animal is divided and quantitatively judged in a standardized way, the memory of the visual space of the animal is further evaluated, and the function of detecting the corresponding top lobe of the brain region is realized.
In some embodiments, in step S2, after the light shielding plate 2 is removed for the first preset time, the arm of the experimental animal still does not pass through the hand stretching opening 13, and the experiment is stopped;
in step S3, after the arm of the experimental animal passes through the hand stretching opening 13 for a second preset time, the grip 4 does not move from the second end to the second end of the experimental lever, and the experiment is stopped.
In the description herein, references to the description of "some embodiments," "other embodiments," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The above description is only a preferred embodiment of the present invention, and for those skilled in the art, the present invention should not be limited by the description of the present invention, which should be interpreted as a limitation.
Claims (10)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911134341.7A CN110833047B (en) | 2019-11-19 | 2019-11-19 | A behavioral experimental device and experimental method for animal visuospatial cognitive memory |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911134341.7A CN110833047B (en) | 2019-11-19 | 2019-11-19 | A behavioral experimental device and experimental method for animal visuospatial cognitive memory |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110833047A CN110833047A (en) | 2020-02-25 |
| CN110833047B true CN110833047B (en) | 2021-08-31 |
Family
ID=69576717
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911134341.7A Active CN110833047B (en) | 2019-11-19 | 2019-11-19 | A behavioral experimental device and experimental method for animal visuospatial cognitive memory |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110833047B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110839558B (en) * | 2019-11-19 | 2021-07-06 | 中国科学院深圳先进技术研究院 | A behavioral experimental device and experimental method for animal auditory association matching |
| CN114097647B (en) * | 2020-08-28 | 2024-04-12 | 深圳先进技术研究院 | Animal working memory monitoring device and monitoring method |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100471465C (en) * | 2006-02-21 | 2009-03-25 | 沈阳医学院 | Auditory and Visual Memory Models and Intelligent Behavior Detection System of Rats |
| CN204192622U (en) * | 2014-10-30 | 2015-03-11 | 海南金港生物技术股份有限公司 | Non-human primate cognition and motor behavior test set |
| CN104663490B (en) * | 2015-03-03 | 2018-10-09 | 广西南宁灵康赛诺科生物科技有限公司 | The automatic device of a set of evaluation primate laboratory animal memory function and the cognitive ability evaluation method of animal |
| WO2016179428A2 (en) * | 2015-05-05 | 2016-11-10 | Dart Neuroscience, Llc | Cognitive test execution and control |
| CN105475163B (en) * | 2015-09-30 | 2018-02-13 | 广西南宁灵康赛诺科生物科技有限公司 | It is a kind of to test primate memory, judgement and the method for executive capability |
-
2019
- 2019-11-19 CN CN201911134341.7A patent/CN110833047B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN110833047A (en) | 2020-02-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110833047B (en) | A behavioral experimental device and experimental method for animal visuospatial cognitive memory | |
| CN110786250B (en) | A behavioral experimental device and experimental method for animal search memory | |
| Veuthey et al. | Single-trial cross-area neural population dynamics during long-term skill learning | |
| Yamaguchi et al. | Identification of animal behavioral strategies by inverse reinforcement learning | |
| Boucher et al. | Inhibitory control in mind and brain: an interactive race model of countermanding saccades. | |
| JP6029069B2 (en) | Animal experiment equipment | |
| Bova et al. | Automated rat single-pellet reaching with 3-dimensional reconstruction of paw and digit trajectories | |
| Lopes et al. | New open-source tools: using Bonsai for behavioral tracking and closed-loop experiments | |
| Desai et al. | MATLAB-based automated patch-clamp system for awake behaving mice | |
| CN1817325A (en) | Mouse hearing and vision memory model and intelligent action inspecting system | |
| CN106489790A (en) | Birds visual cognition learning training system | |
| Atakan et al. | Automated platform for long-term culture and high-content phenotyping of single C. elegans worms | |
| CN103445788B (en) | Behavioral monitoring device and behavioral monitoring system | |
| Gao et al. | A high-throughput zebrafish screening method for visual mutants by light-induced locomotor response | |
| CN110839558B (en) | A behavioral experimental device and experimental method for animal auditory association matching | |
| US20220392017A1 (en) | Image processing for standardizing size and shape of organisms | |
| CN212415732U (en) | An experimental device for measuring animal gait characteristics | |
| Elices et al. | An electrophysiological and kinematic model of Paramecium, the “swimming neuron” | |
| Goss et al. | Quantifying social roles in multi-animal videos using subject-aware deep-learning | |
| Gomez et al. | Microfluidic and computational tools for neurodegeneration studies | |
| WO2024197253A2 (en) | Fish embryo systems and methods for using same | |
| CN105475163B (en) | It is a kind of to test primate memory, judgement and the method for executive capability | |
| WO2007023392A2 (en) | System and method for assessing cognitive function and measuring treatment efficacy | |
| Younk et al. | Quantifying defensive behavior and threat response through integrated headstage accelerometry | |
| Taffoni et al. | A mechatronic platform for behavioral analysis on nonhuman primates |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20250522 Address after: 518000 Guangdong Province, Shenzhen City, Guangming District, Guangming Street, Dongzhou Community, Guangdian North Road 298, Lingxinyu Technology Building 1102 Patentee after: Shenzhen Lingao Biotechnology Co.,Ltd. Country or region after: China Address before: 1068 No. 518055 Guangdong city of Shenzhen province Nanshan District Shenzhen University city academy Avenue Patentee before: SHENZHEN INSTITUTES OF ADVANCED TECHNOLOGY CHINESE ACADEMY OF SCIENCES Country or region before: China |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20250624 Address after: 518132 Guangdong Province Shenzhen City Guangming District Fenghuang Street Fenghuang Community Guanxiang Road 2533 A3 Building B601-3 Patentee after: Shenzhen Kangnao Biotechnology Co.,Ltd. Country or region after: China Address before: 518000 Guangdong Province, Shenzhen City, Guangming District, Guangming Street, Dongzhou Community, Guangdian North Road 298, Lingxinyu Technology Building 1102 Patentee before: Shenzhen Lingao Biotechnology Co.,Ltd. Country or region before: China |