CN220308067U - Mouse behavioural exploration device - Google Patents

Abstract

The utility model provides a mouse behavioural exploration device, which comprises a test box body; the test assembly is arranged in the test box body and comprises an initial platform, a reward platform, a partition plate and a platform lower channel, wherein reward food is arranged on the reward platform, the partition plate is arranged between the initial platform and the reward platform, air holes are formed in the partition plate, and the platform lower channel is arranged in the lower space of the initial platform and is communicated with the initial platform and the reward platform; the monitoring assembly is arranged outside the test box body and is used for monitoring the condition of the mice in the test box body. The device has simple structure and convenient operation, and can explore the natures and the capacities of the mice in all aspects such as emotion, evasion response, difficulty in foraging, etc.

Description

A mouse behavioral exploration device

Technical field

The utility model relates to the technical field of laboratory mouse behavior detection, and specifically relates to a mouse behavior exploration device.

Background technique

Ethology is the science that specializes in the study of animal behavior. The research content is wide-ranging, including animal emotional expression, communication, socialization, learning, escape responses, etc. The ability to identify the environment and direction to find advantages and avoid disadvantages is very important for the survival of animals. In many cognitive science paradigms, previous studies have found that the mouse brain can process a variety of information. Especially when a mouse is injured in a certain location, the mouse will remember the environment and location at that time and move to the next location. Avoid this location in future actions.

Among the devices used to test the spatial cognitive ability of mice, the Y-maze device is currently widely used. However, the Y maze is more biased towards testing the spatial memory ability of mice. This device is a simple test on the same plane and does not include changes in the environment where the mice are located, and the mice's emotions and escape during the process of environmental changes. The nature and abilities of mice in various aspects such as reactions, difficult choices when foraging, etc. are currently unclear, and there is currently a lack of observation and training devices for mice’s foraging abilities and awareness.

Utility model content

The purpose of this utility model is to provide a mouse behavior exploration device in view of the shortcomings of the existing technology.

The specific technical solutions are as follows:

A mouse behavior exploration device, including a test box;

The test component is provided in the test box. The test component includes a starting platform, a reward platform, a partition and a lower channel of the platform. The reward platform is provided with reward food. The partition is provided between the starting platform and the platform. Between the reward platforms, ventilation holes are provided on the partitions, and the lower channel of the platform is provided in the lower space of the starting platform and connects the starting platform and the reward platform;

A monitoring component is provided outside the test box, and the monitoring component is used to monitor the condition of the mice in the test box.

Optionally, the lower channel of the platform includes a first inclined plate, a second inclined plate and a horizontal plate provided between the first inclined plate and the second inclined plate, and the first inclined plate is in contact with the starting platform. The entrance is connected, and the second inclined plate is connected with the exit on the reward platform.

Optionally, the test box is also provided with a water inlet for introducing water into the lower space of the starting platform, the horizontal plate is provided with a leakage hole, and the test box is also provided with a water outlet. .

Optionally, the test box is also provided with a heating tube for heating water introduced into the lower space of the starting platform.

Optionally, a telescopic device is provided between the first inclined plate and the second inclined plate and the horizontal plate, and the telescopic device is used to adjust the height of the horizontal plate.

Optionally, the test box is made of transparent glass or plastic.

Optionally, the monitoring component includes a first camera and a second camera, the first camera is located directly above the partition, and the second camera is located directly between the starting platform and the reward platform.

Optionally, the upper end of the test box is open.

Compared with the existing technology, the beneficial effects of this utility model are:

This device is provided with a platform, a reward platform, a partition and a lower platform channel. The partition separates the starting platform and the reward platform. The lower platform channel is located in the lower space of the starting platform and connects the starting platform and the reward platform. This makes the device no longer a simple test on the same plane. The difficulty has been improved to a certain extent. The food seems to be in front of you but cannot be touched. The mice need to find a more hidden path and respond to the mice's emotions and escape reactions throughout the process. , difficult choices when foraging, etc. to explore various aspects of nature and abilities, detect spatial reference memory and spatial working memory related to the hippocampus and prefrontal brain areas of mice, and successfully find out the discriminative learning ability and spatial memory ability of mice The test can also explore anxiety and depression in mice.

Description of the drawings

Figure 1 is a schematic diagram of the overall structure of a mouse behavioral exploration device provided by an embodiment of the present invention.

In the picture: 1. Test box; 10. Water inlet; 11. Water outlet; 12. Heating pipe; 2. Starting platform; 3. Reward platform; 4. Partition; 40. Ventilation hole; 5. Lower platform channel ; 51. First inclined plate; 52. Second inclined plate; 53. Horizontal plate; 54. Telescopic device; 6. Monitoring component; 61. First camera; 62. Second camera.

Detailed ways

The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only part of the embodiments of the present utility model, not all implementations. example. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without any creative work fall within the scope of protection of the present utility model.

It should be noted that, as long as there is no conflict, the embodiments and features of the embodiments of the present invention can be combined with each other.

The present utility model will be further described below in conjunction with the accompanying drawings and specific embodiments, but shall not be used as a limitation of the present utility model.

The mouse behavior exploration device provided in the utility model, with reference to Figure 1, includes a test box 1;

The test component is located in the test box 1. The test component includes a starting platform 2, a reward platform 3, a partition 4 and a lower channel 5 of the platform. The reward platform 3 is provided with reward food, and the partition 4 is provided on the starting platform 2. between the platform and the reward platform 3, and there are ventilation holes 40 on the partition 4, the lower channel 5 of the platform is provided in the lower space of the starting platform 2 and connects the starting platform 2 and the reward platform 3;

The monitoring component 6 is provided outside the test box 1. The monitoring component 6 is used to monitor the condition of the mice in the test box 1.

During the test, place the mouse on the starting platform 2 and place reward food on the reward platform 3. The partition 4 separates the starting platform 2 and the reward platform 3. The taste of the food is separated from the side of the reward platform 3. The ventilation holes 40 of the plate 4 are uploaded to the actual platform, and the mice can smell the smell of food. However, due to the obstruction of the partition 4 on the same plane, the mice need to explore the correct path to find the reward platform 3, that is, Enter the lower space through the platform passage and reach the bonus platform 3 at the other end.

This experiment can be regarded as an advanced version of the Y-maze experiment. The device is no longer a simple test on the same plane. The difficulty has been improved to a certain extent. The food seems to be in front of you but cannot be touched. The mice need to find a more hidden path. , analyze the mice's feeding strategies, such as the time and number of stays on the platform, the time and number of times to find the platform channel, the time to sequentially find food on the reward platform 3, etc., observe the mouse's exploration path, and analyze the mouse's Foraging ability and consciousness, detecting the spatial reference memory and spatial working memory related to the hippocampus and prefrontal brain areas of mice, successfully finding a test for the discriminative learning ability and spatial memory ability of mice, and also achieving the test of anxiety and depression in mice The exploration of emotions is blocked by partition 4. The mice can smell the smell of food, but cannot obtain the food. The anxiety and depression of the mice can be analyzed by observing the behavior and path of the mice.

The lower channel 5 of the platform includes a first inclined plate 51, a second inclined plate 52 and a horizontal plate 53 located between the first inclined plate 51 and the second inclined plate 52. The first inclined plate 51 and the entrance on the starting platform 2 The second inclined plate 52 is connected with the outlet on the reward platform 3. Specifically, the test box 1 is also provided with a water inlet 10 that allows water to enter the lower space, and the horizontal plate 53 is provided with a leakage hole for testing. The box 1 is also provided with a water outlet hole 11; after the mice can find the correct passing path through certain training in the early stage, water is injected into the bottom of the device through the water inlet 10, and the water can enter the horizontal plate 53 through the leakage hole. When the test mice pass through, there is water The responses of 53 horizontal plates record the mouse's action path, such as whether it is actively looking for new paths.

A telescopic device 54 is provided between the first inclined plate 51 , the second inclined plate 52 and the horizontal plate 53 . The telescopic device 54 is used to adjust the height of the horizontal plate 53 . The telescopic device 54 can be a cylinder, and the horizontal plate is controlled by the cylinder. 53 height, so that the water level on the horizontal plate 53 can be adjusted, and different states of the mouse can be recorded, such as the state of the mouse treading water, the state of the water covering half of the mouse's body, the state of the mouse with only its head above the water, the state of the horizontal plate 53 When the upper space is completely filled with water (when completely filled with water, the mice need to dive to pass through the lower space), you can observe the mice's reactions to passing paths with different difficulties, and record the mice's action paths, such as whether they are active or not. Looking for new ways, the number of attempts and passage times of mice facing different water storage states, etc., can be used to analyze the mice's emotions, escape reactions, difficult choices when foraging, etc. in various aspects of their nature and abilities during the entire process. To explore, you can also change the reward food on reward platform 3 to further explore which situation will attract mice to make a choice.

The test box 1 is also provided with a heating tube 12 for heating the water flowing in the lower space of the starting platform 2. The reaction of mice passing through the horizontal plate 53 with water under different water temperatures can also be recorded.

In addition, the test box 1 is made of transparent glass or plastic material, and the specific conditions inside the test box 1 can be clearly monitored from the outside of the test box 1 .

The monitoring component 6 includes a first camera 61 and a second camera 62. The first camera 61 is located directly above the partition 4, and the second camera 62 is located directly between the starting platform 2 and the reward platform 3. It can be viewed from the front The activities of mice in test box 1 were recorded in real time from above and from the side.

The upper end of the test box 1 is open, which can ensure the air required for normal activities of mice in the test box 1.

The above descriptions are only preferred embodiments of the present invention, and do not limit the implementation and protection scope of the present invention. Those skilled in the art should be able to realize that any application of the contents of the description and illustrations of the present invention Solutions resulting from equivalent substitutions and obvious changes shall be included in the protection scope of the present utility model.

Claims (8)

1. A mouse behavioral exploration device, characterized by comprising: Test box; The test component is provided in the test box. The test component includes a starting platform, a reward platform, a partition and a lower channel of the platform. The reward platform is provided with reward food. The partition is provided between the starting platform and the platform. Between the reward platforms, ventilation holes are provided on the partitions, and the lower channel of the platform is provided in the lower space of the starting platform and connects the starting platform and the reward platform; A monitoring component is provided outside the test box, and the monitoring component is used to monitor the condition of the mice in the test box. 2. The mouse behavior exploration device according to claim 1, characterized in that the lower channel of the platform includes a first inclined plate, a second inclined plate, and an opening between the first inclined plate and the second inclined plate. A horizontal plate, the first inclined plate is connected to the entrance on the starting platform, and the second inclined plate is connected to the exit on the reward platform. 3. The mouse behavior exploration device according to claim 2, characterized in that the test box is also provided with a water inlet for introducing water into the lower space of the starting platform, and the horizontal plate is A leakage hole is provided, and a water outlet hole is also provided on the test box. 4. The mouse behavioral exploration device according to claim 3, wherein the test box is further provided with a heating tube for heating the water introduced into the lower space of the starting platform. 5. The mouse behavioral exploration device according to claim 2, characterized in that a telescopic device is provided between the first inclined plate, the second inclined plate and the horizontal plate, and the telescopic device is used to adjust the level. The height of the board. 6. The mouse behavioral exploration device according to claim 1, wherein the test box is made of transparent glass or plastic. 7. The mouse behavior exploration device according to claim 1, wherein the monitoring component includes a first camera and a second camera, the first camera is located directly above the partition, and the second camera The camera is set facing the position between the starting platform and the reward platform. 8. The mouse behavioral exploration device according to claim 1, wherein the upper end of the test box is open.