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ATE557084T1 - Modifizierte dna-polymerasen zur verbesserten inkorporation von nukleotidanaloga - Google Patents

Modifizierte dna-polymerasen zur verbesserten inkorporation von nukleotidanaloga

Info

Publication number
ATE557084T1
ATE557084T1 AT10180445T AT10180445T ATE557084T1 AT E557084 T1 ATE557084 T1 AT E557084T1 AT 10180445 T AT10180445 T AT 10180445T AT 10180445 T AT10180445 T AT 10180445T AT E557084 T1 ATE557084 T1 AT E557084T1
Authority
AT
Austria
Prior art keywords
nucleotide analogs
dna polymerases
improved incorporation
modified dna
incorporation
Prior art date
Application number
AT10180445T
Other languages
English (en)
Inventor
Geoffrey Paul Smith
David Mark Dunstan Bailey
David James Earnshaw
Raquel Maria Sanches
Harold Swerdlow
Original Assignee
Illumina Cambridge Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=29226911&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE557084(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Illumina Cambridge Ltd filed Critical Illumina Cambridge Ltd
Application granted granted Critical
Publication of ATE557084T1 publication Critical patent/ATE557084T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT10180445T 2003-09-11 2004-09-10 Modifizierte dna-polymerasen zur verbesserten inkorporation von nukleotidanaloga ATE557084T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB0321306.3A GB0321306D0 (en) 2003-09-11 2003-09-11 Modified polymerases for improved incorporation of nucleotide analogues

Publications (1)

Publication Number Publication Date
ATE557084T1 true ATE557084T1 (de) 2012-05-15

Family

ID=29226911

Family Applications (2)

Application Number Title Priority Date Filing Date
AT04768438T ATE532856T1 (de) 2003-09-11 2004-09-10 Modifizierte polymerasen des typs b zum verbesserten einbau von nukleotidanaloga
AT10180445T ATE557084T1 (de) 2003-09-11 2004-09-10 Modifizierte dna-polymerasen zur verbesserten inkorporation von nukleotidanaloga

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT04768438T ATE532856T1 (de) 2003-09-11 2004-09-10 Modifizierte polymerasen des typs b zum verbesserten einbau von nukleotidanaloga

Country Status (8)

Country Link
US (7) US8460910B2 (de)
EP (2) EP1664287B1 (de)
JP (1) JP5706057B2 (de)
AT (2) ATE532856T1 (de)
DK (1) DK2325304T3 (de)
GB (1) GB0321306D0 (de)
HK (1) HK1156353A1 (de)
WO (1) WO2005024010A1 (de)

Families Citing this family (402)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE356222T1 (de) 2000-10-06 2007-03-15 Univ Columbia Massives parallelverfahren zur dekodierung von dna und rna
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
DK3363809T3 (da) 2002-08-23 2020-05-04 Illumina Cambridge Ltd Modificerede nukleotider til polynukleotidsekvensering
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
CA2557818A1 (en) * 2004-03-03 2005-09-15 The Trustees Of Columbia University In The City Of New York Photocleavable fluorescent nucleotides for dna sequencing on chip constructed by site-specific coupling chemistry
US20070048748A1 (en) * 2004-09-24 2007-03-01 Li-Cor, Inc. Mutant polymerases for sequencing and genotyping
GB0509508D0 (en) * 2005-05-10 2005-06-15 Solexa Ltd Improved polymerases
WO2006120433A1 (en) 2005-05-10 2006-11-16 Solexa Limited Improved polymerases
WO2007002204A2 (en) 2005-06-21 2007-01-04 The Trustees Of Columbia University In The City Of New York Pyrosequencing methods and related compostions
US8796432B2 (en) * 2005-10-31 2014-08-05 The Trustees Of Columbia University In The City Of New York Chemically cleavable 3'-o-allyl-DNTP-allyl-fluorophore fluorescent nucleotide analogues and related methods
US7982029B2 (en) 2005-10-31 2011-07-19 The Trustees Of Columbia University In The City Of New York Synthesis of four color 3′O-allyl, modified photocleavable fluorescent nucleotides and related methods
US20090088332A1 (en) * 2005-11-21 2009-04-02 Jingyue Ju Multiplex Digital Immuno-Sensing Using a Library of Photocleavable Mass Tags
WO2007123744A2 (en) 2006-03-31 2007-11-01 Solexa, Inc. Systems and devices for sequence by synthesis analysis
ES2404665T3 (es) * 2006-09-06 2013-05-28 Medical Research Council Polimerasas de ADN que incorporan análogos de nucleótidos marcados con colorante
DK2076592T3 (en) 2006-10-23 2017-07-24 Medical Res Council POLYMERASE
KR100777230B1 (ko) * 2006-11-30 2007-11-28 한국해양연구원 써모코커스 유래 돌연변이 ｄｎａ 중합효소들 및 그의유전자들
WO2008069973A2 (en) 2006-12-01 2008-06-12 The Trustees Of Columbia University In The City Of New York Four-color dna sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US11035823B2 (en) 2009-03-17 2021-06-15 Qiagen Sciences, Llc Methods and devices for sequencing nucleic acids in smaller batches
US11940413B2 (en) 2007-02-05 2024-03-26 IsoPlexis Corporation Methods and devices for sequencing nucleic acids in smaller batches
US8612161B2 (en) 2008-03-19 2013-12-17 Intelligent Biosystems Inc. Methods and compositions for base calling nucleic acids
US8481259B2 (en) 2007-02-05 2013-07-09 Intelligent Bio-Systems, Inc. Methods and devices for sequencing nucleic acids in smaller batches
EP3431615A3 (de) 2007-10-19 2019-02-20 The Trustees of Columbia University in the City of New York Dna-sequenzierung mit nichtfluoreszierenden, reversiblen nukleotidterminatoren und spaltbaren, etikettmodifizierten nukleotidterminatoren
EP2940029B1 (de) 2007-10-19 2023-11-29 The Trustees of Columbia University in the City of New York Design und synthese von spaltbaren fluoreszierenden nukleotiden als reversible terminatoren zur dna-sequenzierung durch synthese
US8617811B2 (en) 2008-01-28 2013-12-31 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US9017973B2 (en) 2008-03-19 2015-04-28 Intelligent Biosystems, Inc. Methods and compositions for incorporating nucleotides
US20110045489A1 (en) * 2008-04-22 2011-02-24 New England Biolabs, Inc. Polymerases for Incorporating Modified Nucleotides
US8039817B2 (en) 2008-05-05 2011-10-18 Illumina, Inc. Compensator for multiple surface imaging
EP2294214A2 (de) * 2008-05-07 2011-03-16 Illumina, Inc. Zusammensetzungen und verfahren zur bereitstellung von substanzen an und aus einem array
US8486865B2 (en) * 2008-11-03 2013-07-16 The Regents Of The University Of California Methods for detecting modification resistant nucleic acids
WO2010068702A2 (en) * 2008-12-10 2010-06-17 Illumina, Inc. Methods and compositions for hybridizing nucleic acids
EP2607496B1 (de) 2008-12-23 2014-07-16 Illumina, Inc. Für Nukleinsäuresequenzierungsprotokolle geeignete Verfahren
US8640555B2 (en) 2009-02-09 2014-02-04 Bioaccel Performance
EP2427572B1 (de) * 2009-05-01 2013-08-28 Illumina, Inc. Sequenzierungsverfahren
US20110262903A1 (en) * 2010-02-26 2011-10-27 Life Technologies Corporation Modified Proteins and Methods of Making and Using Same
US20120202276A1 (en) 2010-02-26 2012-08-09 Life Technologies Corporation Modified Proteins and Methods of Making and Using Same
US8951940B2 (en) 2010-04-01 2015-02-10 Illumina, Inc. Solid-phase clonal amplification and related methods
CA2794522C (en) 2010-04-05 2019-11-26 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
GB201007384D0 (en) * 2010-04-30 2010-06-16 Medical Res Council Enzymes
US8993737B2 (en) 2010-08-25 2015-03-31 Pacific Biosciences, Inc. Phospholinked dye analogs with an amino acid linker
US9029103B2 (en) 2010-08-27 2015-05-12 Illumina Cambridge Limited Methods for sequencing polynucleotides
US8483969B2 (en) 2010-09-17 2013-07-09 Illuminia, Inc. Variation analysis for multiple templates on a solid support
WO2012050920A1 (en) 2010-09-29 2012-04-19 Illumina, Inc. Compositions and methods for sequencing nucleic acids
US8753816B2 (en) 2010-10-26 2014-06-17 Illumina, Inc. Sequencing methods
EP3928867B1 (de) 2010-10-27 2024-07-17 Illumina, Inc. Mikrobauelemente und biosensorkassetten zur biologischen oder chemischen analyse sowie systeme und verfahren dafür
US8575071B2 (en) 2010-11-03 2013-11-05 Illumina, Inc. Reducing adapter dimer formation
EP2635679B1 (de) 2010-11-05 2017-04-19 Illumina, Inc. Verknüpfung von sequenzstücken mit gepaarten code-tags
WO2012074855A2 (en) 2010-11-22 2012-06-07 The Regents Of The University Of California Methods of identifying a cellular nascent rna transcript
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
AU2012316218B2 (en) 2011-09-26 2016-03-17 Gen-Probe Incorporated Algorithms for sequence determinations
US10378051B2 (en) 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
WO2013070627A2 (en) 2011-11-07 2013-05-16 Illumina, Inc. Integrated sequencing apparatuses and methods of use
WO2013085710A2 (en) 2011-12-09 2013-06-13 Illumina, Inc. Expanded radix for polymeric tags
WO2013117595A2 (en) 2012-02-07 2013-08-15 Illumina Cambridge Limited Targeted enrichment and amplification of nucleic acids on a support
NO2694769T3 (de) 2012-03-06 2018-03-03
US9444880B2 (en) 2012-04-11 2016-09-13 Illumina, Inc. Cloud computing environment for biological data
WO2013173844A1 (en) 2012-05-18 2013-11-21 Pacific Biosciences Of California, Inc. Heteroarylcyanine dyes
US9315864B2 (en) 2012-05-18 2016-04-19 Pacific Biosciences Of California, Inc. Heteroarylcyanine dyes with sulfonic acid substituents
WO2014008448A1 (en) 2012-07-03 2014-01-09 Sloan Kettering Institute For Cancer Research Quantitative assessment of human t-cell repertoire recovery after allogeneic hematopoietic stem cell transplantation
NL2017959B1 (en) 2016-12-08 2018-06-19 Illumina Inc Cartridge assembly
JP6478445B2 (ja) * 2012-09-28 2019-03-06 東洋紡株式会社 改変された耐熱性ｄｎａポリメラーゼ
CA2886974C (en) 2012-10-17 2021-06-29 Spatial Transcriptomics Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
US9116139B2 (en) 2012-11-05 2015-08-25 Illumina, Inc. Sequence scheduling and sample distribution techniques
US20160138063A1 (en) * 2013-01-04 2016-05-19 John Chaput Methds and compositions for replication of threose nucleic acids
US9805407B2 (en) 2013-01-25 2017-10-31 Illumina, Inc. Methods and systems for using a cloud computing environment to configure and sell a biological sample preparation cartridge and share related data
US9650406B2 (en) 2013-02-28 2017-05-16 Centrillion Technology Holdings Corporation Reversible terminator molecules and methods of their use
CA2902992C (en) 2013-03-08 2020-08-25 Illumina Cambridge Ltd Rhodamine compounds and their use as fluorescent labels
WO2014135221A1 (en) 2013-03-08 2014-09-12 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
DK3553175T3 (da) 2013-03-13 2021-08-23 Illumina Inc Fremgangsmåde til fremstilling af et nukleinsyresekvenseringsbibliotek
AU2013382024B2 (en) * 2013-03-14 2019-01-31 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
WO2014144883A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
KR102230831B1 (ko) 2013-03-15 2021-03-22 일루미나 케임브리지 리미티드 변형된 뉴클레오사이드 또는 뉴클레오타이드
US11384377B2 (en) 2013-04-02 2022-07-12 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US9279149B2 (en) 2013-04-02 2016-03-08 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
US9771613B2 (en) 2013-04-02 2017-09-26 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acid
US11331643B2 (en) 2013-04-02 2022-05-17 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US8808989B1 (en) 2013-04-02 2014-08-19 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
US10683536B2 (en) 2013-04-02 2020-06-16 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
WO2014210225A1 (en) 2013-06-25 2014-12-31 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
US9352315B2 (en) 2013-09-27 2016-05-31 Taiwan Semiconductor Manufacturing Company, Ltd. Method to produce chemical pattern in micro-fluidic structure
US10540783B2 (en) * 2013-11-01 2020-01-21 Illumina, Inc. Image analysis useful for patterned objects
PL3077943T3 (pl) 2013-12-03 2020-11-30 Illumina, Inc. Sposoby i układy do analizy danych obrazowych
ES2971473T3 (es) 2013-12-10 2024-06-05 Illumina Inc Biosensores para análisis biológicos o químicos y métodos para la fabricación de los mismos
US10537889B2 (en) 2013-12-31 2020-01-21 Illumina, Inc. Addressable flow cell using patterned electrodes
BR112016018547B1 (pt) 2014-02-13 2023-12-12 Illumina, Inc Método para processar informações genômicas
EP3910069A1 (de) 2014-02-18 2021-11-17 Illumina, Inc. Verfahren und zusammensetzungen zur dna-profilierung
EP3116651B1 (de) 2014-03-11 2020-04-22 Illumina, Inc. Wegwerfbare, integrierte mikrofluidische kartusche und verfahren zur herstellung davon
GB201408077D0 (en) 2014-05-07 2014-06-18 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
AU2015259024B2 (en) 2014-05-16 2021-07-01 Illumina, Inc. Nucleic acid synthesis techniques
WO2015183871A1 (en) 2014-05-27 2015-12-03 Illumina, Inc. Systems and methods for biochemical analysis including a base instrument and a removable cartridge
US20150353989A1 (en) 2014-06-09 2015-12-10 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
JP6737710B2 (ja) 2014-06-27 2020-08-12 イルミナ インコーポレイテッド ヌクレオチドアナログの取り込みを改善するための修飾ポリメラーゼ
US9469862B2 (en) * 2014-08-19 2016-10-18 Arizona Board Of Regents On Behalf Of Arizona State University Modified polymerases for replication of threose nucleic acids
US9963687B2 (en) 2014-08-27 2018-05-08 New England Biolabs, Inc. Fusion polymerase and method for using the same
EP3778891A1 (de) 2014-08-27 2021-02-17 New England Biolabs, Inc. Synthonbildung
WO2016040602A1 (en) 2014-09-11 2016-03-17 Epicentre Technologies Corporation Reduced representation bisulfite sequencing using uracil n-glycosylase (ung) and endonuclease iv
CN107002051A (zh) 2014-09-30 2017-08-01 亿明达股份有限公司 用于核苷酸类似物的改善的掺入的经修饰的聚合酶
US9897791B2 (en) 2014-10-16 2018-02-20 Illumina, Inc. Optical scanning systems for in situ genetic analysis
GB201419731D0 (en) 2014-11-05 2014-12-17 Illumina Cambridge Ltd Sequencing from multiple primers to increase data rate and density
ES2768762T3 (es) 2014-11-05 2020-06-23 Illumina Cambridge Ltd Reducción del daño al ADN durante la preparación de muestras y secuenciación usando agentes quelantes sideróforos
ES2802405T3 (es) 2014-11-11 2021-01-19 Illumina Cambridge Ltd Métodos y matrices para producir y secuenciar agrupaciones monoclonales de ácido nucleico
US10329545B2 (en) 2014-11-14 2019-06-25 Illumina, Inc. Polymerases
SG11201706504RA (en) 2015-02-10 2017-09-28 Illumina Inc Methods and compositions for analyzing cellular components
US10576471B2 (en) 2015-03-20 2020-03-03 Illumina, Inc. Fluidics cartridge for use in the vertical or substantially vertical position
WO2016156845A1 (en) 2015-03-31 2016-10-06 Illumina Cambridge Limited Surface concatamerization of templates
ES2955916T3 (es) 2015-04-10 2023-12-11 Spatial Transcriptomics Ab Análisis múltiplex de especímenes biológicos de ácidos nucleicos espacialmente distinguidos
EP3091026B1 (de) 2015-05-08 2019-02-20 Centrillion Technology Holdings Corporation Disulfidvernetzte reversible terminatoren
GB201508858D0 (en) 2015-05-22 2015-07-01 Illumina Cambridge Ltd Polymethine compounds with long stokes shifts and their use as fluorescent labels
ES2789349T3 (es) 2015-05-29 2020-10-26 Illumina Cambridge Ltd Utilización mejorada de cebadores de superficie en grupos
US10640809B2 (en) 2015-05-29 2020-05-05 Epicentre Technologies Corporation Methods of analyzing nucleic acids
EP3878974A1 (de) 2015-07-06 2021-09-15 Illumina Cambridge Limited Probenvorbereitung zur nukleinsäureamplifikation
EP3957747A1 (de) 2015-07-27 2022-02-23 Illumina, Inc. Räumliche zuordnung von nukleinsäuresequenzen
PL3334839T3 (pl) 2015-08-14 2021-08-02 Illumina, Inc. Systemy i sposoby wykorzystujące czujniki reagujące na pole magnetyczne do określania informacji genetycznej
US10976334B2 (en) 2015-08-24 2021-04-13 Illumina, Inc. In-line pressure accumulator and flow-control system for biological or chemical assays
EP4006150A1 (de) 2015-09-09 2022-06-01 QIAGEN GmbH Polymeraseenzym
US10450598B2 (en) 2015-09-11 2019-10-22 Illumina, Inc. Systems and methods for obtaining a droplet having a designated concentration of a substance-of-interest
GB201516987D0 (en) 2015-09-25 2015-11-11 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
US10577643B2 (en) 2015-10-07 2020-03-03 Illumina, Inc. Off-target capture reduction in sequencing techniques
WO2017062965A1 (en) * 2015-10-08 2017-04-13 Arizona Board Of Regents On Behalf Of Arizona State University Highly sensitive optical sensor for polymerase screening
US11421264B2 (en) 2015-11-06 2022-08-23 IsoPlexis Corporation Thiol-containing cleave reagents and oxidative wash
AU2016349457B2 (en) 2015-11-06 2022-09-22 Qiagen Sciences Llc Nucleotide analogues
WO2017087724A1 (en) 2015-11-17 2017-05-26 Omniome, Inc. Methods for determining sequence profiles
EP3904514A1 (de) 2016-07-22 2021-11-03 Oregon Health & Science University Einzellige gesamtgenombibliotheken und kombinatorische indexierungsverfahren zur herstellung davon
US20230207048A1 (en) 2016-09-22 2023-06-29 Illumina, Inc. Somatic copy number variation detection
US10385214B2 (en) 2016-09-30 2019-08-20 Illumina Cambridge Limited Fluorescent dyes and their uses as biomarkers
TWI781669B (zh) 2016-10-14 2022-10-21 美商伊路米納有限公司 卡匣總成
JP2020504074A (ja) 2016-12-22 2020-02-06 イルミナ ケンブリッジ リミテッド クマリン化合物および蛍光標識としてのそれらの使用
IL267836B2 (en) 2017-01-04 2023-09-01 Complete Genomics Inc Stepwise sequence determination by unlabeled reversible terminations or natural nucleotides
BR112019013886A2 (pt) 2017-01-06 2020-03-03 Illumina, Inc. Método para determinar os valores de cor corrigidos, e, sequenciador de ácidos nucleicos
US20200090784A1 (en) 2017-01-17 2020-03-19 Illumina, Inc. Oncogenic splice variant determination
CN110249059B (zh) 2017-01-20 2021-04-13 欧姆尼欧美公司 核酸的等位基因特异性捕获
EP3565900B1 (de) 2017-01-20 2021-04-07 Omniome, Inc. Genotypisierung durch polymerasebindung
WO2018148724A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from pyrococcus furiosus
WO2018148727A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from 9°n
EP3580334A1 (de) * 2017-02-13 2019-12-18 QIAGEN GmbH Polymeraseenzyme aus phage t4
US20220127587A1 (en) * 2017-02-13 2022-04-28 IsoPlexis Corporation Polymerase enzyme from pyrococcus furiosus
WO2018148723A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from pyrococcus abyssi
EP3580352A1 (de) * 2017-02-13 2019-12-18 Qiagen Sciences, LLC Polymeraseenzym aus pyrococcus abyssi
US20200002689A1 (en) 2017-02-13 2020-01-02 Qiagen Sciences, Llc Polymerase enzyme from 9°n
WO2018148726A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from phage t4
EP4053294A1 (de) 2017-03-24 2022-09-07 Life Technologies Corporation Polynukleotidadapter und verfahren zur verwendung davon
DK3615691T3 (da) 2017-04-23 2021-07-26 Illumina Inc Sammensætninger og fremgangsmåder til forbedring af prøveidentifikation i indekserede nukleinsyrebiblioteker
CN110785497A (zh) 2017-04-23 2020-02-11 伊鲁米纳剑桥有限公司 用于改进编索引的核酸文库中的样品鉴定的组合物和方法
WO2018197945A1 (en) 2017-04-23 2018-11-01 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
SG10202108175RA (en) 2017-06-07 2021-09-29 Univ Oregon Health & Science Single cell whole genome libraries for methylation sequencing
CN111032882A (zh) 2017-06-20 2020-04-17 伊鲁米那股份有限公司 解决扩增反应中低效的方法和组合物
GB201711219D0 (en) 2017-07-12 2017-08-23 Illumina Cambridge Ltd Short pendant arm linkers for nucleotides in sequencing applications
WO2019027767A1 (en) 2017-07-31 2019-02-07 Illumina Inc. SEQUENCING SYSTEM COMPRISING AGGREGATION OF MULTIPLEXED BIOLOGICAL SAMPLES
ES2999650T3 (en) 2017-08-01 2025-02-26 Illumina Inc Spatial indexing of genetic material and library preparation using hydrogel beads and flow cells
EP3545106B1 (de) 2017-08-01 2022-01-19 Helitec Limited Verfahren zur anreicherung und bestimmung von zielnukleotidsequenzen
CN118086480A (zh) 2017-08-01 2024-05-28 深圳华大智造科技有限公司 核酸测序方法
WO2019030149A1 (en) 2017-08-07 2019-02-14 Dna Script VARIANTS OF FAMILY A DNA POLYMERASE AND USES THEREOF
WO2019051253A1 (en) * 2017-09-08 2019-03-14 Sigma-Aldrich Co. Llc DNA MODIFIED POLYMERASES
RU2020113600A (ru) 2017-09-20 2021-10-20 Ридженерон Фармасьютикалз, Инк. Способы иммунотерапии пациентов, опухоли которых характеризуются высокой нагрузкой пассажирскими генными мутациями
GB201716931D0 (en) 2017-10-16 2017-11-29 Illumina Cambridge Ltd New fluorescent compounds and their use as biomarkers
CN118126816A (zh) 2017-11-06 2024-06-04 伊鲁米那股份有限公司 核酸索引化技术
CN110800061B (zh) 2017-11-16 2024-10-11 伊鲁米那股份有限公司 用于确定微卫星不稳定性的系统和方法
AU2018375785A1 (en) 2017-11-30 2019-12-12 Illumina, Inc. Validation methods and systems for sequence variant calls
WO2019136376A1 (en) 2018-01-08 2019-07-11 Illumina, Inc. High-throughput sequencing with semiconductor-based detection
US10752887B2 (en) 2018-01-08 2020-08-25 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
AU2019205797A1 (en) 2018-01-08 2019-12-19 Illumina, Inc. Systems and devices for high-throughput sequencing with semiconductor-based detection
US10844430B2 (en) 2018-01-24 2020-11-24 Qiagen Sciences, Llc DNA sequencing reaction additive
RU2739571C1 (ru) 2018-03-09 2020-12-25 Иллюмина Кембридж Лимитед Генерализованное стохастическое секвенирование сверхразрешения
US12188012B2 (en) 2018-04-02 2025-01-07 Illumina, Inc. Compositions and methods for making controls for sequence-based genetic testing
IL271454B1 (en) 2018-05-17 2024-12-01 Illumina Inc High-throughput single-cell sequencing with reduced amplification bias
CN111247248A (zh) 2018-06-04 2020-06-05 伊鲁米纳公司 高通量单细胞转录组文库及制备和使用方法
NL2021377B1 (en) 2018-07-03 2020-01-08 Illumina Inc Interposer with first and second adhesive layers
US20230323447A1 (en) 2018-08-28 2023-10-12 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic dna in a biological sample
WO2020047005A1 (en) 2018-08-28 2020-03-05 10X Genomics, Inc. Resolving spatial arrays
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
WO2020056044A1 (en) 2018-09-11 2020-03-19 Singular Genomics Systems, Inc. Modified archaeal family b polymerases
WO2020060811A1 (en) 2018-09-17 2020-03-26 Omniome, Inc. Engineered polymerases for improved sequencing
CN113195511A (zh) 2018-09-28 2021-07-30 生捷科技控股公司 二硫键连接的可逆终止剂
CA3103719A1 (en) 2018-10-31 2020-05-07 Illumina, Inc. Polymerases, compositions, and methods of use
NL2022043B1 (en) 2018-11-21 2020-06-03 Akershus Univ Hf Tagmentation-Associated Multiplex PCR Enrichment Sequencing
CA3103739A1 (en) 2018-12-05 2020-06-11 Illumina, Inc. Polymerases, compositions, and methods of use
US20220025447A1 (en) 2018-12-10 2022-01-27 10X Genomics, Inc. Generating spatial arrays with gradients
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
EP3931354A1 (de) 2019-02-28 2022-01-05 10X Genomics, Inc. Profilierung von biologischen analyten mit räumlich barcodierten oligonukleotid-arrays
NL2023327B1 (en) 2019-03-01 2020-09-17 Illumina Inc Multiplexed fluorescent detection of analytes
SG11202102530QA (en) 2019-03-01 2021-04-29 Illumina Inc High-throughput single-nuclei and single-cell libraries and methods of making and of using
SG11202012555UA (en) 2019-03-01 2021-01-28 Illumina Cambridge Ltd Exocyclic amine-substituted coumarin compounds and their uses as fluorescent labels
WO2020178231A1 (en) 2019-03-01 2020-09-10 Illumina, Inc. Multiplexed fluorescent detection of analytes
IL279660B2 (en) 2019-03-01 2024-10-01 Illumina Cambridge Ltd Tertiary amine substituted coumarin compounds and their uses as fluorescent labels
EP3938538A1 (de) 2019-03-15 2022-01-19 10X Genomics, Inc. Verfahren zur verwendung von räumlichen anordnungen für einzelzellsequenzierung
NL2023316B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based sequencing
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
NL2023314B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based quality scoring
NL2023312B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based base calling
NL2023310B1 (en) 2019-03-21 2020-09-28 Illumina Inc Training data generation for artificial intelligence-based sequencing
US11676685B2 (en) 2019-03-21 2023-06-13 Illumina, Inc. Artificial intelligence-based quality scoring
NL2023311B9 (en) 2019-03-21 2021-03-12 Illumina Inc Artificial intelligence-based generation of sequencing metadata
WO2020191390A2 (en) 2019-03-21 2020-09-24 Illumina, Inc. Artificial intelligence-based quality scoring
EP3887542A1 (de) 2019-03-22 2021-10-06 10X Genomics, Inc. Dreidimensionale räumliche analyse
US11421271B2 (en) 2019-03-28 2022-08-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing using photoswitchable labels
US11423306B2 (en) 2019-05-16 2022-08-23 Illumina, Inc. Systems and devices for characterization and performance analysis of pixel-based sequencing
US11593649B2 (en) 2019-05-16 2023-02-28 Illumina, Inc. Base calling using convolutions
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
US11512295B2 (en) 2019-09-12 2022-11-29 Singular Genomics Systems, Inc. Modified thermoccocus polymerases
US20220403436A1 (en) 2019-09-23 2022-12-22 Dna Script Increasing Long-Sequence Yields in Template-Free Enzymatic Synthesis of Polynucleotides
US11514575B2 (en) 2019-10-01 2022-11-29 10X Genomics, Inc. Systems and methods for identifying morphological patterns in tissue samples
US20210139867A1 (en) 2019-11-08 2021-05-13 Omniome, Inc. Engineered polymerases for improved sequencing by binding
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
EP4025711A2 (de) 2019-11-08 2022-07-13 10X Genomics, Inc. Erhöhung der spezifität einer analytbindung
US12154266B2 (en) 2019-11-18 2024-11-26 10X Genomics, Inc. Systems and methods for binary tissue classification
AU2020388573A1 (en) 2019-11-21 2022-06-23 10X Genomics, Inc, Spatial analysis of analytes
CN114829369A (zh) 2019-11-27 2022-07-29 伊鲁米纳剑桥有限公司 含环辛四烯的染料和组合物
KR20220111315A (ko) 2019-12-18 2022-08-09 에프. 호프만-라 로슈 아게 연속 표지화 방식을 사용하는 합성에 의한 시퀀싱 방법
AU2020407641A1 (en) 2019-12-19 2021-09-23 Illumina, Inc. High-throughput single-cell libraries and methods of making and of using
WO2021133849A1 (en) 2019-12-23 2021-07-01 10X Genomics, Inc. Methods for spatial analysis using rna-templated ligation
CN115038794A (zh) 2019-12-23 2022-09-09 10X基因组学有限公司 在基于分区的测定中使用固定生物样品的组合物和方法
CN110938611B (zh) * 2019-12-31 2021-05-04 莫纳(武汉)生物科技有限公司 一种dna聚合酶及其制备方法和应用
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US12112833B2 (en) 2020-02-04 2024-10-08 10X Genomics, Inc. Systems and methods for index hopping filtering
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
EP4107284A1 (de) 2020-02-17 2022-12-28 10X Genomics, Inc. In situ-analyse von chromatinwechselwirkung
AU2021224871A1 (en) 2020-02-20 2022-09-08 Illumina, Inc. Artificial intelligence-based many-to-many base calling
US20210265018A1 (en) 2020-02-20 2021-08-26 Illumina, Inc. Knowledge Distillation and Gradient Pruning-Based Compression of Artificial Intelligence-Based Base Caller
US20210265015A1 (en) 2020-02-20 2021-08-26 Illumina, Inc. Hardware Execution and Acceleration of Artificial Intelligence-Based Base Caller
US20210265016A1 (en) 2020-02-20 2021-08-26 Illumina, Inc. Data Compression for Artificial Intelligence-Based Base Calling
JP2023514749A (ja) 2020-02-21 2023-04-07 １０エックス ジェノミクス インコーポレイテッド 統合型インサイチュ空間的アッセイのための方法および組成物
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11034942B1 (en) 2020-02-27 2021-06-15 Singular Genomics Systems, Inc. Modified pyrococcus polymerases and uses thereof
US12188085B2 (en) 2020-03-05 2025-01-07 10X Genomics, Inc. Three-dimensional spatial transcriptomics with sequencing readout
EP4121554B1 (de) 2020-03-18 2024-10-30 MGI Tech Co., Ltd. Wiederherstellung der phase in massiv paralleler sequenzierung
WO2021216708A1 (en) 2020-04-22 2021-10-28 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
US20230203592A1 (en) 2020-05-05 2023-06-29 Akershus Universitetssykehus Hf Compositions and methods for characterizing bowel cancer
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
CA3174298A1 (en) 2020-05-08 2021-11-11 Sven BILKE Genome sequencing and detection techniques
CN115916967A (zh) 2020-05-12 2023-04-04 伊鲁米纳公司 使用重组末端脱氧核苷酸转移酶生成具有经修饰的碱基的核酸
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
ES2989052T3 (es) 2020-05-22 2024-11-25 10X Genomics Inc Medición espacio-temporal simultánea de la expresión génica y la actividad celular
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
ES2981265T3 (es) 2020-06-08 2024-10-08 10X Genomics Inc Métodos para determinar un margen quirúrgico y métodos de uso del mismo
WO2021252617A1 (en) 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
ES2999535T3 (en) 2020-06-10 2025-02-26 10X Genomics Inc Methods for determining a location of an analyte in a biological sample
BR112022026056A2 (pt) 2020-06-22 2023-03-07 Illumina Cambridge Ltd Nucleosídeos e nucleotídeos com grupo bloqueador 3? acetal
CN116034166A (zh) 2020-06-25 2023-04-28 10X基因组学有限公司 Dna甲基化的空间分析
JP2023532231A (ja) 2020-06-30 2023-07-27 イルミナ インコーポレイテッド 傷なしｄｎａを生成するための触媒的に制御された合成による配列決定
US12168801B1 (en) 2020-07-02 2024-12-17 10X Genomics, Inc. Hybrid/capture probe designs for full-length cDNA
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
CN116018412A (zh) 2020-07-08 2023-04-25 Illumina公司 作为转座体载体的小珠
US11981964B2 (en) 2020-07-28 2024-05-14 Illumina Cambridge Limited Substituted coumarin dyes and uses as fluorescent labels
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US20220067489A1 (en) 2020-08-28 2022-03-03 Illumina, Inc. Detecting and Filtering Clusters Based on Artificial Intelligence-Predicted Base Calls
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
CA3179883A1 (en) 2020-12-02 2022-06-09 Illumina Software, Inc. System and method for detection of genetic alterations
US20220186300A1 (en) 2020-12-11 2022-06-16 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
US20220195517A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Long stokes shift chromenoquinoline dyes and uses in sequencing applications
US20220195516A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Methods, systems and compositions for nucleic acid sequencing
US20220195196A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Alkylpyridinium coumarin dyes and uses in sequencing applications
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US20220195518A1 (en) 2020-12-22 2022-06-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
EP4488385A3 (de) 2021-01-26 2025-02-19 10x Genomics, Inc. Nukleinsäureanalogsonden zur in-situ-analyse
US20220282319A1 (en) 2021-03-03 2022-09-08 10X Genomics, Inc. Analyte detection in situ using nucleic acid origami
WO2022197752A1 (en) 2021-03-16 2022-09-22 Illumina, Inc. Tile location and/or cycle based weight set selection for base calling
WO2022194244A1 (en) * 2021-03-19 2022-09-22 Mgi Tech Co., Ltd. Polymerases for efficient incorporation of nucleotides with 3'-phosphate and other 3'-terminators
MX2023011660A (es) 2021-03-31 2023-12-11 Illumina Cambridge Ltd Tecnicas de secuenciacion de genoteca de acido nucleico con deteccion de dimero adaptador.
CA3183578A1 (en) 2021-03-31 2022-10-06 Illumina Inc. Artificial intelligence-based base caller with contextual awareness
WO2022213027A1 (en) 2021-04-02 2022-10-06 Illumina, Inc. Machine-learning model for detecting a bubble within a nucleotide-sample slide for sequencing
WO2022221425A1 (en) 2021-04-14 2022-10-20 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
CN117242192A (zh) 2021-04-14 2023-12-15 伊鲁米纳新加坡有限公司 多核苷酸瓶刷状聚合物的合成
US12217829B2 (en) 2021-04-15 2025-02-04 Illumina, Inc. Artificial intelligence-based analysis of protein three-dimensional (3D) structures
FR3121936A1 (fr) 2021-04-20 2022-10-21 Naos Institute Of Life Science Methode de criblage d’ingredients et de compositions topiques ecobiologiques, et son utilisation pour realiser un soin cosmetique de restauration du microbiote cutane
CN117377775A (zh) 2021-04-29 2024-01-09 因美纳有限公司 用于核酸表征的扩增技术
EP4334327A1 (de) 2021-05-05 2024-03-13 Illumina Cambridge Limited Fluoreszierende farbstoffe mit kondensierten bis-bor-heterocyclen und verwendungen in der sequenzierung
MX2023012659A (es) 2021-05-20 2023-12-07 Illumina Inc Composiciones y metodos para secuenciación por síntesis.
CN117396613A (zh) 2021-06-01 2024-01-12 10X基因组学有限公司 用于分析物检测和探针解析的方法和组合物
CN117751197A (zh) 2021-06-02 2024-03-22 10X基因组学有限公司 使用不对称可环化探针的样品分析
US20220403450A1 (en) 2021-06-03 2022-12-22 Illumina Software, Inc. Systems and methods for sequencing nucleotides using two optical channels
WO2022256503A1 (en) 2021-06-03 2022-12-08 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
US12139727B2 (en) 2021-06-18 2024-11-12 Element Biosciences, Inc. Engineered polymerases
CA3223112A1 (en) * 2021-06-18 2022-12-22 Mark AMBROSO Engineered polymerases
CN117597453A (zh) 2021-06-23 2024-02-23 伊鲁米纳公司 用于测序试剂的组合物、方法、试剂盒、料筒和系统
WO2023278609A1 (en) 2021-06-29 2023-01-05 Illumina, Inc. Self-learned base caller, trained using organism sequences
WO2023278184A1 (en) 2021-06-29 2023-01-05 Illumina, Inc. Methods and systems to correct crosstalk in illumination emitted from reaction sites
JP2024529836A (ja) 2021-06-29 2024-08-14 イルミナ インコーポレイテッド ゲノム座標の確信度分類を生成するための機械学習モデル
JP2024527307A (ja) 2021-06-29 2024-07-24 イルミナ インコーポレイテッド ヌクレオチド塩基コール及び塩基コール品質を決定するための信号対雑音比メトリック
US20230005253A1 (en) 2021-07-01 2023-01-05 Illumina, Inc. Efficient artificial intelligence-based base calling of index sequences
CN117651855A (zh) 2021-07-13 2024-03-05 10X基因组学有限公司 用于制备具有可控厚度的聚合基质的方法
US20230027409A1 (en) 2021-07-13 2023-01-26 Illumina, Inc. Methods and systems for real time extraction of crosstalk in illumination emitted from reaction sites
JP2024532004A (ja) 2021-07-19 2024-09-04 イルミナ インコーポレイテッド ベースコールのための補間及び適合による強度抽出
US11455487B1 (en) 2021-10-26 2022-09-27 Illumina Software, Inc. Intensity extraction and crosstalk attenuation using interpolation and adaptation for base calling
US20230021577A1 (en) 2021-07-23 2023-01-26 Illumina Software, Inc. Machine-learning model for recalibrating nucleotide-base calls
IL309786A (en) 2021-07-28 2024-02-01 Illumina Inc Quality score calibration of BASECALLING systems
EP4450640A3 (de) 2021-07-30 2024-11-27 10x Genomics, Inc. Verfahren und zusammensetzungen zur in-situ-synchronisierung von reaktionen
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
CN118043476A (zh) 2021-08-03 2024-05-14 10X基因组学有限公司 核酸多联体及其稳定和/或压缩方法
WO2023014741A1 (en) 2021-08-03 2023-02-09 Illumina Software, Inc. Base calling using multiple base caller models
US12077789B2 (en) 2021-08-14 2024-09-03 Illumina, Inc. Polymerases, compositions, and methods of use
WO2023023484A1 (en) 2021-08-16 2023-02-23 10X Genomics, Inc. Probes comprising a split barcode region and methods of use
CN117580961A (zh) 2021-09-01 2024-02-20 Illumina公司 用于加速碱基判读的幅度调制
EP4509614A2 (de) 2021-09-01 2025-02-19 10X Genomics, Inc. Verfahren, zusammensetzungen und kits zur blockierung einer erfassungssonde auf einer räumlichen anordnung
WO2023044229A1 (en) 2021-09-17 2023-03-23 Illumina, Inc. Automatically identifying failure sources in nucleotide sequencing from base-call-error patterns
US20230095961A1 (en) 2021-09-21 2023-03-30 Illumina, Inc. Graph reference genome and base-calling approach using imputed haplotypes
WO2023049212A2 (en) 2021-09-22 2023-03-30 Illumina, Inc. State-based base calling
EP4305195A2 (de) 2021-12-01 2024-01-17 10X Genomics, Inc. Verfahren, zusammensetzungen und systeme für verbesserten in-situ-nachweis von analyten und raumanalyse
JP2024543762A (ja) 2021-12-02 2024-11-26 イルミナ インコーポレイテッド ヌクレオチド塩基コールを決定するためのクラスタ固有シグナル補正の生成
US20230183799A1 (en) 2021-12-10 2023-06-15 Illumina, Inc. Parallel sample and index sequencing
CA3222829A1 (en) 2021-12-16 2023-06-22 Gabriele CANZI Methods for metal directed cleavage of surface-bound polynucleotides
EP4453256A1 (de) 2021-12-20 2024-10-30 Illumina, Inc. Periodatzusammensetzungen und verfahren zur chemischen spaltung von oberflächengebundenen polynukleotiden
US20240401127A1 (en) 2021-12-20 2024-12-05 Illumina, Inc. Periodate compositions and methods for chemical cleavage of surface-bound polynucleotides
US20230215515A1 (en) 2021-12-23 2023-07-06 Illumina Software, Inc. Facilitating secure execution of external workflows for genomic sequencing diagnostics
WO2023122363A1 (en) 2021-12-23 2023-06-29 Illumina Software, Inc. Dynamic graphical status summaries for nucelotide sequencing
WO2023129898A2 (en) 2021-12-27 2023-07-06 10X Genomics, Inc. Methods and compositions for rolling circle amplification
US20230207050A1 (en) 2021-12-28 2023-06-29 Illumina Software, Inc. Machine learning model for recalibrating nucleotide base calls corresponding to target variants
JP2025504332A (ja) 2021-12-29 2025-02-12 イルミナ インコーポレイテッド ゲノム分析アプリケーションのためのバリアント分析モデルバージョンの自動切り替え
US20230279475A1 (en) 2022-01-21 2023-09-07 10X Genomics, Inc. Multiple readout signals for analyzing a sample
US12006518B2 (en) 2022-02-18 2024-06-11 Element Biosciences, Inc. Engineered polymerases with reduced sequence-specific errors
WO2023164570A1 (en) 2022-02-23 2023-08-31 Insitro, Inc. Pooled optical screening and transcriptional measurements of cells comprising barcoded genetic perturbations
EP4483372A1 (de) 2022-02-25 2025-01-01 Illumina, Inc. Kalibrierungssequenzen für nucelotidsequenzierung
EP4483371A1 (de) 2022-02-25 2025-01-01 Illumina, Inc. Maschinenlernmodelle zur erkennung und anpassung von werten für nukleotidmethylierungsebenen
WO2023175029A1 (en) 2022-03-15 2023-09-21 Illumina, Inc. Concurrent sequencing of hetero n-mer polynucleotides
EP4500536A1 (de) 2022-03-25 2025-02-05 Illumina, Inc. Sequenz-zu-sequenz-basisaufruf
EP4499862A1 (de) 2022-03-28 2025-02-05 Illumina, Inc. Markiertes avidin und sequenzierungsverfahren
EP4499758A1 (de) 2022-03-29 2025-02-05 Illumina Inc. Chromenochinolinfarbstoffe und verwendungen in der sequenzierung
US20230313294A1 (en) 2022-03-30 2023-10-05 Illumina Cambridge Limited Methods for chemical cleavage of surface-bound polynucleotides
AU2023244351A1 (en) 2022-03-31 2024-01-18 Illumina, Inc. Nucleosides and nucleotides with 3' vinyl blocking group useful in sequencing by synthesis
EP4499870A1 (de) 2022-03-31 2025-02-05 Illumina, Inc. Zusammensetzungen und verfahren zur verbesserung von sequenzierungssignalen
CN119032271A (zh) 2022-04-01 2024-11-26 10X基因组学有限公司 用于靶向掩蔽自发荧光的组合物和方法
CN119234045A (zh) 2022-04-06 2024-12-31 10X基因组学有限公司 用于多重细胞分析的方法
CN119053701A (zh) 2022-04-07 2024-11-29 因美纳有限公司 经改变的胞苷脱氨酶和使用方法
WO2023196528A1 (en) 2022-04-08 2023-10-12 Illumina, Inc. Aptamer dynamic range compression and detection techniques
US20230340571A1 (en) 2022-04-26 2023-10-26 Illumina, Inc. Machine-learning models for selecting oligonucleotide probes for array technologies
WO2023209606A1 (en) 2022-04-29 2023-11-02 Illumina Cambridge Limited Methods and systems for encapsulating lyophilised microspheres
WO2023215612A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Analysis of antigen and antigen receptor interactions
WO2023220627A1 (en) 2022-05-10 2023-11-16 Illumina Software, Inc. Adaptive neural network for nucelotide sequencing
CN119546761A (zh) 2022-05-18 2025-02-28 因美纳有限公司 大小受控的核酸片段的制备
CN119301275A (zh) 2022-05-31 2025-01-10 伊路米纳有限公司 用于核酸测序的组合物和方法
US20240035071A1 (en) 2022-06-17 2024-02-01 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
CN119422201A (zh) 2022-06-24 2025-02-11 因美纳有限公司 通过智能鉴定候选分割组并评分来改进分割-读段比对
US20230420075A1 (en) 2022-06-27 2023-12-28 Illumina Software, Inc. Accelerators for a genotype imputation model
KR20250028287A (ko) 2022-06-27 2025-02-28 일루미나, 인코포레이티드 구조 변이 그래프 게놈의 생성 및 구현
WO2024006705A1 (en) 2022-06-27 2024-01-04 Illumina Software, Inc. Improved human leukocyte antigen (hla) genotyping
US20230416279A1 (en) 2022-06-28 2023-12-28 Illumina Cambridge Limited Fluorescent dyes containing fused tetracyclic bis-boron heterocycle and uses in sequencing
US20240038327A1 (en) 2022-07-26 2024-02-01 Illumina Software, Inc. Rapid single-cell multiomics processing using an executable file
WO2024036304A1 (en) 2022-08-12 2024-02-15 10X Genomics, Inc. Puma1 polymerases and uses thereof
EP4511510A1 (de) 2022-08-16 2025-02-26 10X Genomics, Inc. Ap50 polymerasen und deren verwendungen
WO2024039516A1 (en) 2022-08-19 2024-02-22 Illumina, Inc. Third dna base pair site-specific dna detection
WO2024059852A1 (en) 2022-09-16 2024-03-21 Illumina, Inc. Cluster segmentation and conditional base calling
CN118974830A (zh) 2022-09-29 2024-11-15 因美纳有限公司 用于推算靶变体的靶变体参考组
WO2024073047A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides
WO2024069581A1 (en) 2022-09-30 2024-04-04 Illumina Singapore Pte. Ltd. Helicase-cytidine deaminase complexes and methods of use
WO2024073043A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides
US20240120027A1 (en) 2022-09-30 2024-04-11 Illumina, Inc. Machine-learning model for refining structural variant calls
US20240141427A1 (en) 2022-09-30 2024-05-02 Illumina, Inc. Polymerases, compositions, and methods of use
US20240140939A1 (en) 2022-09-30 2024-05-02 Illumina Cambridge Limited Compositions and methods for reducing photo damage during sequencing
WO2024077096A1 (en) 2022-10-05 2024-04-11 Illumina, Inc. Integrating variant calls from multiple sequencing pipelines utilizing a machine learning architecture
WO2024081649A1 (en) 2022-10-11 2024-04-18 Illumina, Inc. Detecting and correcting methylation values from methylation sequencing assays
US20240191297A1 (en) 2022-10-14 2024-06-13 10X Genomics, Inc. Methods, compositions, and systems for assessing biological sample quality
US20240167081A1 (en) 2022-11-08 2024-05-23 10X Genomics,Inc. Immobilization methods and compositions for in situ detection
US20240158852A1 (en) 2022-11-16 2024-05-16 10X Genomics, Inc. Methods and compositions for assessing performance of in situ assays
WO2024118791A1 (en) 2022-11-30 2024-06-06 Illumina, Inc. Accurately predicting variants from methylation sequencing data
WO2024123866A1 (en) 2022-12-09 2024-06-13 Illumina, Inc. Nucleosides and nucleotides with 3´ blocking groups and cleavable linkers
US20240218437A1 (en) 2022-12-16 2024-07-04 10X Genomics, Inc. Methods and compositions for assessing performance
US20240212791A1 (en) 2022-12-21 2024-06-27 Illumina, Inc. Context-dependent base calling
WO2024137860A1 (en) 2022-12-21 2024-06-27 10X Genomics, Inc. Methods for tethering ribonucleic acids in biological samples
CN119095983A (zh) 2022-12-22 2024-12-06 伊路米纳有限公司 用于边合成边测序的过渡金属催化剂组合物和方法
CN119095982A (zh) 2022-12-22 2024-12-06 伊路米纳有限公司 用于边合成边测序的钯催化剂组合物和方法
WO2024145154A1 (en) 2022-12-27 2024-07-04 Illumina, Inc. Methods of sequencing using 3´ allyl blocked nucleotides
US20240263219A1 (en) 2023-01-06 2024-08-08 10X Genomics, Inc. Methods and compositions for in situ analysis of variant sequences
CN119452420A (zh) 2023-02-06 2025-02-14 因美纳有限公司 确定和去除簇间光干扰
AU2024235961A1 (en) 2023-03-10 2025-01-09 Illumina, Inc. Aptamer detection techniques
CN119452044A (zh) 2023-03-29 2025-02-14 因美纳公司 萘酰亚胺染料以及其在核酸测序中的用途
US20240327909A1 (en) 2023-03-30 2024-10-03 Illumina, Inc. Compositions and methods for nucleic acid sequencing
WO2024206848A1 (en) 2023-03-30 2024-10-03 Illumina, Inc. Tandem repeat genotyping
AU2024266370A1 (en) 2023-05-03 2025-01-16 Illumina, Inc. Machine learning model for recalibrating genotype calls from existing sequencing data files
US20240368678A1 (en) 2023-05-03 2024-11-07 10X Genomics, Inc. Methods and compositions for spatial assay
WO2024249200A1 (en) 2023-05-26 2024-12-05 Illumina, Inc. Methods for preserving methylation status during clustering
US20240404624A1 (en) 2023-05-31 2024-12-05 Illumina, Inc. Structural variant alignment and variant calling by utilizing a structural-variant reference genome
WO2024249973A2 (en) 2023-06-02 2024-12-05 Illumina, Inc. Linking human genes to clinical phenotypes using graph neural networks
WO2025006565A1 (en) 2023-06-27 2025-01-02 Illumina, Inc. Variant calling with methylation-level estimation
WO2025006460A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Systems and methods of sequencing polynucleotides with modified bases
WO2025006466A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Systems and methods of sequencing polynucleotides with four labeled nucleotides
WO2025006464A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Systems and methods of sequencing polynucleotides with alternative scatterplots
WO2025006874A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Machine-learning model for recalibrating genotype calls corresponding to germline variants and somatic mosaic variants
WO2025029831A1 (en) 2023-07-31 2025-02-06 10X Genomics, Inc. Methods and systems for targeted rna cleavage and target rna-primed rolling circle amplification
US20250075269A1 (en) 2023-08-31 2025-03-06 Illumina, Inc. Compositions and methods for nucleic acid sequencing
WO2025049331A2 (en) 2023-08-31 2025-03-06 Illumina, Inc. Aptamer detection techniques
WO2025049720A2 (en) 2023-08-31 2025-03-06 Illumina, Inc. Aptamer dynamic range compression and detection techniques
WO2025054389A1 (en) 2023-09-07 2025-03-13 Illumina, Inc. Identification of methylated cytosine using landmarks
WO2025050463A1 (en) 2023-09-08 2025-03-13 The Regents Of The University Of Michigan MICRORNA-DERIVED RNAs AND POLYPEPTIDES AND USES THEREOF

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5756334A (en) * 1990-04-26 1998-05-26 New England Biolabs, Inc. Thermostable DNA polymerase from 9°N-7 and methods for producing the same
AU2804597A (en) 1996-04-15 1997-11-07 University Of Alberta Synthesis of fluorophore-labeled dna
EP1218546A4 (de) * 1999-09-30 2005-01-05 New England Biolabs Inc Einbau von modifizierten nukleotiden durch die archaeon-dna-polymerasen und damit zusammenhängende methoden
US20030228616A1 (en) * 1999-10-29 2003-12-11 Stratagene DNA polymerase mutants with reverse transcriptase activity
US8268605B2 (en) * 1999-10-29 2012-09-18 Agilent Technologies, Inc. Compositions and methods utilizing DNA polymerases
AU1239901A (en) * 1999-10-29 2001-05-14 Stratagene Compositions and methods utilizing dna polymerases
US6329178B1 (en) * 2000-01-14 2001-12-11 University Of Washington DNA polymerase mutant having one or more mutations in the active site
AU2007201277B2 (en) 2000-05-24 2011-10-13 Third Wave Technologies, Inc. Detection of RNA
EP1373889A2 (de) 2000-07-31 2004-01-02 Maxygen, Inc. Biosensoren, reagenzien und diagnostische anwendungen gerichteter evolution
WO2002101358A2 (en) 2001-06-11 2002-12-19 Illumina, Inc. Multiplexed detection methods
DK3363809T3 (da) * 2002-08-23 2020-05-04 Illumina Cambridge Ltd Modificerede nukleotider til polynukleotidsekvensering
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
US20070048748A1 (en) 2004-09-24 2007-03-01 Li-Cor, Inc. Mutant polymerases for sequencing and genotyping
WO2006120433A1 (en) 2005-05-10 2006-11-16 Solexa Limited Improved polymerases

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