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SG11202102530QA - High-throughput single-nuclei and single-cell libraries and methods of making and of using - Google Patents

High-throughput single-nuclei and single-cell libraries and methods of making and of using

Info

Publication number
SG11202102530QA
SG11202102530QA SG11202102530QA SG11202102530QA SG11202102530QA SG 11202102530Q A SG11202102530Q A SG 11202102530QA SG 11202102530Q A SG11202102530Q A SG 11202102530QA SG 11202102530Q A SG11202102530Q A SG 11202102530QA SG 11202102530Q A SG11202102530Q A SG 11202102530QA
Authority
SG
Singapore
Prior art keywords
nuclei
making
methods
cell libraries
throughput
Prior art date
Application number
SG11202102530QA
Inventor
Sanjay Srivatsan
Jose Mcfaline-Figueroa
Vijay Ramani
Junyue Cao
Gregory Booth
Jay Shendure
Cole Trapnell
Frank J Steemers
Original Assignee
Illumina Inc
Univ Washington
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc, Univ Washington filed Critical Illumina Inc
Publication of SG11202102530QA publication Critical patent/SG11202102530QA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
SG11202102530QA 2019-03-01 2020-03-02 High-throughput single-nuclei and single-cell libraries and methods of making and of using SG11202102530QA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962812853P 2019-03-01 2019-03-01
PCT/US2020/020637 WO2020180778A1 (en) 2019-03-01 2020-03-02 High-throughput single-nuclei and single-cell libraries and methods of making and of using

Publications (1)

Publication Number Publication Date
SG11202102530QA true SG11202102530QA (en) 2021-04-29

Family

ID=70110324

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202102530QA SG11202102530QA (en) 2019-03-01 2020-03-02 High-throughput single-nuclei and single-cell libraries and methods of making and of using

Country Status (12)

Country Link
US (1) US20220033805A1 (en)
EP (1) EP3837365A1 (en)
JP (3) JP7637390B2 (en)
CN (1) CN113302300A (en)
AU (1) AU2020232618B2 (en)
BR (1) BR112021006234A2 (en)
CA (1) CA3113841A1 (en)
IL (1) IL281829A (en)
MX (1) MX2021003746A (en)
NZ (1) NZ773860A (en)
SG (1) SG11202102530QA (en)
WO (1) WO2020180778A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020206285A1 (en) * 2019-04-05 2020-10-08 Board Of Regents, The University Of Texas System Methods and applications for cell barcoding
KR20220118295A (en) 2019-12-19 2022-08-25 일루미나, 인코포레이티드 High Throughput Single Cell Libraries, and Methods of Making and Using the Same
US12497651B2 (en) 2020-11-23 2025-12-16 Pleno, Inc. Encoded dual-probe endonuclease assays
CN114107459B (en) * 2021-11-11 2022-12-06 浙江大学 High-throughput single cell sequencing method based on oligonucleotide chain hybridization marker
CN116694730B (en) * 2022-02-28 2025-02-14 南方科技大学 A method for constructing a single-cell open chromatin and transcriptome co-sequencing library
IL292281A (en) 2022-04-14 2023-11-01 Yeda Res & Dev Methods of single cell rna-sequencing
US20240281455A1 (en) * 2023-02-16 2024-08-22 Oracle International Corporation Tuning-free unsupervised anomaly detection based on distance to nearest normal point
CN117497037B (en) * 2023-11-17 2024-08-16 上海倍谙基生物科技有限公司 Culture medium component sensitivity analysis method based on generalized linear model
IL309573A (en) 2023-12-20 2025-07-01 Yeda res & development co ltd Methods for solving cell transition dynamics

Family Cites Families (82)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
AU622426B2 (en) 1987-12-11 1992-04-09 Abbott Laboratories Assay using template-dependent nucleic acid probe reorganization
CA1341584C (en) 1988-04-06 2008-11-18 Bruce Wallace Method of amplifying and detecting nucleic acid sequences
WO1989009835A1 (en) 1988-04-08 1989-10-19 The Salk Institute For Biological Studies Ligase-based amplification method
EP0379559B1 (en) 1988-06-24 1996-10-23 Amgen Inc. Method and reagents for detecting nucleic acid sequences
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
EP0425563B1 (en) 1988-07-20 1996-05-15 David Segev Process for amplifying and detecting nucleic acid sequences
US5185243A (en) 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
CA2044616A1 (en) 1989-10-26 1991-04-27 Roger Y. Tsien Dna sequencing
KR950013953B1 (en) 1990-01-26 1995-11-18 애보트 래보라토리즈 Method of amplifying target nucleic acids applicable to ligase chain reactions
US5573907A (en) 1990-01-26 1996-11-12 Abbott Laboratories Detecting and amplifying target nucleic acids using exonucleolytic activity
US5223414A (en) 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
WO1995021271A1 (en) 1994-02-07 1995-08-10 Molecular Tool, Inc. Ligase/polymerase-mediated genetic bit analysistm of single nucleotide polymorphisms and its use in genetic analysis
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
AU687535B2 (en) 1994-03-16 1998-02-26 Gen-Probe Incorporated Isothermal strand displacement nucleic acid amplification
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
EP3034626A1 (en) 1997-04-01 2016-06-22 Illumina Cambridge Limited Method of nucleic acid sequencing
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
AR021833A1 (en) 1998-09-30 2002-08-07 Applied Research Systems METHODS OF AMPLIFICATION AND SEQUENCING OF NUCLEIC ACID
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
DE60131194T2 (en) 2000-07-07 2008-08-07 Visigen Biotechnologies, Inc., Bellaire SEQUENCE PROVISION IN REAL TIME
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AR031640A1 (en) 2000-12-08 2003-09-24 Applied Research Systems ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS IN A SOLID SUPPORT
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
EP2607369B1 (en) 2002-08-23 2015-09-23 Illumina Cambridge Limited Modified nucleotides for polynucleotide sequencing
AU2003272438B2 (en) 2002-09-20 2009-04-02 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
CA2528577C (en) 2003-06-20 2012-08-07 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
JP2007525571A (en) 2004-01-07 2007-09-06 ソレクサ リミテッド Modified molecular array
GB0400584D0 (en) 2004-01-12 2004-02-11 Solexa Ltd Nucleic acid chacterisation
JP2008513782A (en) 2004-09-17 2008-05-01 パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド Apparatus and method for molecular analysis
WO2006064199A1 (en) 2004-12-13 2006-06-22 Solexa Limited Improved method of nucleotide detection
JP4990886B2 (en) 2005-05-10 2012-08-01 ソレックサ リミテッド Improved polymerase
CN101466847B (en) 2005-06-15 2014-02-19 考利达基因组股份有限公司 Single-molecule arrays for genetic and chemical analysis
US20090264299A1 (en) 2006-02-24 2009-10-22 Complete Genomics, Inc. High throughput genome sequencing on DNA arrays
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
JP5180845B2 (en) 2006-02-24 2013-04-10 カリダ・ジェノミックス・インコーポレイテッド High-throughput genomic sequencing on DNA arrays
US20080009420A1 (en) 2006-03-17 2008-01-10 Schroth Gary P Isothermal methods for creating clonal single molecule arrays
EP3722409A1 (en) 2006-03-31 2020-10-14 Illumina, Inc. Systems and devices for sequence by synthesis analysis
US8343746B2 (en) 2006-10-23 2013-01-01 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
WO2008070352A2 (en) 2006-10-27 2008-06-12 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
EP3285067B1 (en) 2006-12-14 2022-06-22 Life Technologies Corporation Apparatus for measuring analytes using fet arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US9233253B2 (en) 2012-01-16 2016-01-12 Greatbatch Ltd. EMI filtered co-connected hermetic feedthrough, feedthrough capacitor and leadwire assembly for an active implantable medical device
WO2010003132A1 (en) 2008-07-02 2010-01-07 Illumina Cambridge Ltd. Using populations of beads for the fabrication of arrays on surfaces
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
EP2417249B1 (en) 2009-04-08 2022-06-08 The Regents of The University of California Dna-cell conjugates
WO2011143231A2 (en) * 2010-05-10 2011-11-17 The Broad Institute High throughput paired-end sequencing of large-insert clone libraries
GB2512213B (en) 2010-10-08 2015-02-11 Harvard College High-throughput single cell barcoding
EP2635679B1 (en) 2010-11-05 2017-04-19 Illumina, Inc. Linking sequence reads using paired code tags
US8829171B2 (en) 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
US8778848B2 (en) 2011-06-09 2014-07-15 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
CA3104322C (en) 2011-09-23 2023-06-13 Illumina, Inc. Methods and compositions for nucleic acid sequencing
EP3305400A3 (en) 2011-10-28 2018-06-06 Illumina, Inc. Microarray fabrication system and method
KR102118211B1 (en) 2012-04-03 2020-06-02 일루미나, 인코포레이티드 Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
EP3470530B1 (en) 2013-05-23 2020-11-25 The Board of Trustees of the Leland Stanford Junior University Transposition into native chromatin for analysis ofchromatin
HUE041318T2 (en) 2013-07-01 2019-05-28 Illumina Inc Catalyst-free surface functionalization and polymer grafting
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
CA2965578C (en) 2014-10-31 2024-03-19 Illumina Cambridge Limited Polymers and dna copolymer coatings
JP7001475B2 (en) 2015-02-10 2022-01-19 イラミーナ インコーポレーテッド Methods and compositions for analyzing cellular constituents
KR102709499B1 (en) 2016-07-22 2024-09-24 오레곤 헬스 앤드 사이언스 유니버시티 Single cell whole genome libraries and combinatorial indexing methods of making thereof
GB201622222D0 (en) * 2016-12-23 2017-02-08 Cs Genetics Ltd Reagents and methods for molecular barcoding of nucleic acids of single cells
CN117887804A (en) 2017-02-02 2024-04-16 纽约基因组研究中心公司 Methods and compositions for identifying or quantifying targets in biological samples

Also Published As

Publication number Publication date
JP2022522917A (en) 2022-04-21
MX2021003746A (en) 2021-06-23
WO2020180778A1 (en) 2020-09-10
JP2023062105A (en) 2023-05-02
IL281829A (en) 2021-05-31
CA3113841A1 (en) 2020-09-10
JP7637390B2 (en) 2025-02-28
JP2025014035A (en) 2025-01-28
BR112021006234A2 (en) 2021-09-28
EP3837365A1 (en) 2021-06-23
CN113302300A (en) 2021-08-24
AU2020232618A1 (en) 2021-04-08
WO2020180778A9 (en) 2021-04-08
NZ773860A (en) 2026-01-30
US20220033805A1 (en) 2022-02-03
AU2020232618B2 (en) 2025-11-13
KR20210134598A (en) 2021-11-10

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