AR113177A2 - Polipéptidos que tienen actividad de lisozima y polinucleótidos que los codifican - Google Patents
Polipéptidos que tienen actividad de lisozima y polinucleótidos que los codificanInfo
- Publication number
- AR113177A2 AR113177A2 ARP180102743A ARP180102743A AR113177A2 AR 113177 A2 AR113177 A2 AR 113177A2 AR P180102743 A ARP180102743 A AR P180102743A AR P180102743 A ARP180102743 A AR P180102743A AR 113177 A2 AR113177 A2 AR 113177A2
- Authority
- AR
- Argentina
- Prior art keywords
- polypeptides
- polynucleotides
- lysozyme activity
- code
- lysozyme
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/184—Hormones
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/20—Shaping or working-up of animal feeding-stuffs by moulding, e.g. making cakes or briquettes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/25—Shaping or working-up of animal feeding-stuffs by extrusion
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/46—Hydrolases (3)
- A61K38/47—Hydrolases (3) acting on glycosyl compounds (3.2), e.g. cellulases, lactases
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
- C11B1/102—Production of fats or fatty oils from raw materials by extracting in counter-current; utilisation of an equipment wherein the material is conveyed by a screw
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2462—Lysozyme (3.2.1.17)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01017—Lysozyme (3.2.1.17)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Animal Husbandry (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Birds (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Inorganic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Marine Sciences & Fisheries (AREA)
- Insects & Arthropods (AREA)
- Epidemiology (AREA)
- Immunology (AREA)
- Endocrinology (AREA)
- Oncology (AREA)
Abstract
La presente se refiere a polipéptidos aislados que tienen actividad de lisozima, y a polinucleótidos que codifican los polipéptidos. Además se refiere a construcciones de ácido nucleico, vectores y células huéspedes que comprenden los polinucleótidos, al igual que a métodos para la producción y el uso de los polipéptidos.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP11190690 | 2011-11-25 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR113177A2 true AR113177A2 (es) | 2020-02-05 |
Family
ID=47226164
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP120104418A AR088976A1 (es) | 2011-11-25 | 2012-11-23 | Polipeptidos que tienen actividad de lisozima y polinucleotidos que los codifican |
ARP180102743A AR113177A2 (es) | 2011-11-25 | 2018-09-24 | Polipéptidos que tienen actividad de lisozima y polinucleótidos que los codifican |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP120104418A AR088976A1 (es) | 2011-11-25 | 2012-11-23 | Polipeptidos que tienen actividad de lisozima y polinucleotidos que los codifican |
Country Status (18)
Country | Link |
---|---|
US (7) | US9663775B2 (es) |
EP (2) | EP2782592B1 (es) |
JP (1) | JP6105615B2 (es) |
CN (2) | CN107090445A (es) |
AR (2) | AR088976A1 (es) |
AU (1) | AU2012342456B2 (es) |
BR (2) | BR112014012160B1 (es) |
CA (1) | CA2856593C (es) |
CL (1) | CL2014001317A1 (es) |
DK (1) | DK2782592T3 (es) |
ES (2) | ES2631605T3 (es) |
IN (1) | IN2014CN04586A (es) |
MX (2) | MX353710B (es) |
PE (1) | PE20151745A1 (es) |
PL (1) | PL2782592T3 (es) |
RU (1) | RU2619051C2 (es) |
WO (2) | WO2013076259A2 (es) |
ZA (1) | ZA201403761B (es) |
Families Citing this family (67)
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CA2856593C (en) | 2011-11-25 | 2022-06-07 | Novozymes A/S | Polypeptides having lysozyme activity and polynucleotides encoding same |
MX2016012260A (es) * | 2014-03-28 | 2017-01-06 | Novozymes As | Resolubilizacion de cristales de proteina a bajo ph. |
US20160030528A1 (en) * | 2014-07-01 | 2016-02-04 | Vanderbilt University | Antimicrobial muramidase |
DK3186373T3 (da) * | 2014-08-28 | 2021-03-22 | Renescience As | Solubilisering af kommunalt fast affald med enzymsammensætning |
MX2017016811A (es) | 2015-07-02 | 2018-04-24 | Novozymes As | Metodos para mejorar el rendimiento del animal. |
MX2017017018A (es) | 2015-07-02 | 2018-02-26 | Novozymes As | Composiciones de pienso para animales y usos de las mismas. |
WO2017000922A1 (en) * | 2015-07-02 | 2017-01-05 | Novozymes A/S | Animal feed compositions and uses thereof |
WO2017064092A1 (en) * | 2015-10-13 | 2017-04-20 | Dsm Ip Assets B.V. | Feed additives for aquatic animals comprising essential oils and lysozyme |
CN108289469A (zh) * | 2015-12-02 | 2018-07-17 | 日清奥利友集团株式会社 | 包覆用油脂组合物 |
US11685911B2 (en) | 2016-02-16 | 2023-06-27 | Monaghan Mushroom S Group | Fungal polypeptides having lysozyme activity |
US11130800B2 (en) | 2016-05-20 | 2021-09-28 | Novobind Livestock Therapeutics Inc. | Antibodies against microorganisms and uses thereof |
CN105861468B (zh) * | 2016-06-07 | 2019-12-06 | 江南大学 | 一种n端融合hlh功能域提高猪溶菌酶抗菌性能的方法 |
CN106046173B (zh) * | 2016-06-07 | 2019-05-10 | 江南大学 | 一种n端融合多聚六肽提高猪溶菌酶抗菌性能的方法 |
CN106701373A (zh) * | 2016-12-11 | 2017-05-24 | 深圳市美益洁生物科技有限公司 | 茶具生物酶除菌清洗剂及其制备方法和应用 |
CN110139666B (zh) * | 2016-12-21 | 2024-07-05 | 诺维信公司 | 具有溶菌酶活性的多肽、编码该多肽的多核苷酸及其用途和组合物 |
US10986850B2 (en) | 2016-12-21 | 2021-04-27 | Novozymes A/S | Polypeptides having lysozyme activity, polynucleotides encoding same and uses and compositions thereof |
MY191930A (en) * | 2017-01-04 | 2022-07-19 | Novozymes As | Microbial lysozyme for use in the treatment of irritable bowel syndrome or inflammatory bowel disease |
DK3606936T3 (da) * | 2017-04-03 | 2022-01-10 | Novozymes As | Fremgangsmåde til indvinding |
EP3621452A1 (en) | 2017-05-09 | 2020-03-18 | Novozymes A/S | Animal chew toy with dental care composition |
US11859170B2 (en) * | 2017-05-12 | 2024-01-02 | Novozymes A/S | Polypeptides having lysozyme activity, polynucleotides encoding same and uses and compositions thereof |
US11554190B2 (en) | 2017-08-18 | 2023-01-17 | President And Fellows Of Harvard College | Nanocarriers for the delivery of active ingredients |
EP3679579A1 (en) * | 2017-09-05 | 2020-07-15 | Adaptive Phage Therapeutics, Inc. | Methods to determine the sensitivity profile of a bacterial strain to a therapeutic composition |
CN108251440B (zh) * | 2017-12-18 | 2021-04-13 | 宁波大学 | 缢蛏溶菌酶基因、编码蛋白及重组缢蛏溶菌酶基因工程菌的构建方法和应用 |
EP3727024A1 (en) | 2017-12-20 | 2020-10-28 | DSM IP Assets B.V. | Animal feed compositions comprising muramidase and uses thereof |
US20210076704A1 (en) * | 2017-12-20 | 2021-03-18 | Dsm Ip Assets B.V. | Animal feed compositions and uses thereof |
CN110115776A (zh) * | 2018-02-07 | 2019-08-13 | 沈阳药科大学 | 一种具有抗菌活性的可吸收淀粉微球止血粉及其应用 |
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BR112020021365A2 (pt) | 2018-04-25 | 2021-01-19 | Novozymes A/S | Ração animal, método para melhoria do fator de eficiência de produção europeu, ganho de peso corporal e/ou razão de conversão de ração de um animal monogástrico, uso de pelo menos um probiótico em combinação com um polipeptídeo tendo atividade de muramidase em uma composição de ração, e, composição de ração ou uma composição de pré-mistura, ou um aditivo de ração para animais. |
AU2019339615A1 (en) | 2018-09-11 | 2021-03-04 | Dsm Ip Assets B.V. | Animal feed composition comprising muramidase and use thereof |
EP3849337A1 (en) | 2018-09-11 | 2021-07-21 | DSM IP Assets B.V. | Animal feed composition and use thereof |
CA3108944A1 (en) | 2018-09-11 | 2020-03-19 | Dsm Ip Assets B.V. | Animal feed composition and use thereof |
WO2020053276A1 (en) | 2018-09-11 | 2020-03-19 | Dsm Ip Assets B.V. | Animal feed composition and use thereof |
MX2021002725A (es) | 2018-09-11 | 2021-05-12 | Novozymes As | Granulos estables para composiciones de pienso. |
BR112021004480A2 (pt) | 2018-09-11 | 2021-06-01 | Dsm Ip Assets B.V. | composição de ração animal e uso da mesma |
US20220031815A1 (en) | 2018-09-17 | 2022-02-03 | Dsm Ip Assets B.V. | Animal feed composition and use thereof |
WO2020058225A1 (en) | 2018-09-17 | 2020-03-26 | Dsm Ip Assets B.V. | Animal feed compositions and uses thereof |
BR112021004826A2 (pt) | 2018-09-17 | 2021-06-08 | Dsm Ip Assets B.V. | composições de ração animal e usos das mesmas |
WO2020058228A1 (en) | 2018-09-17 | 2020-03-26 | Dsm Ip Assets B.V. | Animal feed compositions and uses thereof |
WO2020058224A1 (en) | 2018-09-17 | 2020-03-26 | Dsm Ip Assets B.V. | Animal feed compositions and uses thereof |
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US20240122209A1 (en) * | 2019-10-17 | 2024-04-18 | Novozymes A/S | Animal feed compositions and uses thereof |
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CN111378635B (zh) * | 2020-05-29 | 2020-10-16 | 中国农业科学院北京畜牧兽医研究所 | 一种共表达溶菌酶和木聚糖酶的方法、及制备作为替代抗生素的饲料添加剂的方法 |
KR20230057391A (ko) | 2020-08-24 | 2023-04-28 | 노보자임스 에이/에스 | 프럭타나제를 포함하는 구강 관리 조성물 |
WO2022047149A1 (en) | 2020-08-27 | 2022-03-03 | Danisco Us Inc | Enzymes and enzyme compositions for cleaning |
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- 2017-05-26 US US15/606,031 patent/US10119130B2/en active Active
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2018
- 2018-06-29 US US16/023,129 patent/US10829750B2/en active Active
- 2018-09-24 AR ARP180102743A patent/AR113177A2/es unknown
- 2018-09-25 US US16/140,941 patent/US10829751B2/en active Active
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2020
- 2020-08-20 US US16/998,066 patent/US20200399625A1/en not_active Abandoned
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