- Zagreb, Grad Zagreb, Croatia
U životu mladunčadi mačića neonatalno razdoblje predstavlja prva dva do tri tjedna života. U tom razdoblju novorođenčad je u potpunosti ovisna o majci. Mladučad reagira samo na bol, dodir i miris, a razvijene su im samo motoričke... more
U životu mladunčadi mačića neonatalno razdoblje predstavlja prva dva do tri tjedna života. U tom razdoblju novorođenčad je u potpunosti ovisna o majci. Mladučad reagira samo na bol, dodir i miris, a razvijene su im samo motoričke funkcije. Smrtnost mačića u dobi od jednog do sedam dana je do 85 %, što je vrlo visok postotak uginuća. Glavni uzrok neonatalnog mortaliteta je hipoksija, gotovo uvijek praćena s hipotermijom, hipoglikemijom i dehidracijom. Ova četiri hitna stanja najčešće se pojavljuju odjednom i potrebno ih je rješavati pravilnim redoslijedom da ne bi prouzročili veću smrtnost. Za liječenje i dijagnosticiranje ova četiri hitna stanja potrebno je razumjeti fiziologiju novorođenčadi. Novorođenčad prespava 80 % tijekom dana, a jesti trebaju svaka dva do četiri sata. Centar za termoregulaciju nije potpuno razvijen do dobi od šest tjedana. Tjelesna temperatura novorođenčadi ovisi o temperaturi okoliša, imaju mali postotak masnog tkiva i stoga su skloni razvoju hipotermije, ko...
Research Interests: Physics, Humanities, Hypoxia, Neonatology, Hypothermia, and 3 moreHypoglycemia, Dehydration, and Kittens
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The aim of the research was to determine the influence of preventive intrauterine application of ozone spray preparation on shortening days open in Simmental cows. The research was conducted on 120 Simmental cows aged 2-7 years divided in... more
The aim of the research was to determine the influence of preventive intrauterine application of ozone spray preparation on shortening days open in Simmental cows. The research was conducted on 120 Simmental cows aged 2-7 years divided in two groups. The first group consisted of cows (n=60) treated with ozone foam spray (RIGER SPRAY®, Novagen, Italy) application in the body of uterus for 5-7 seconds. The second group of cows (n=60) was the control group. Cows from the first group were observed and treated during early puerperium, 48 hours after parturition. Average milk production (measured in 305 days of lactation), in the first group (treated with Riger spray) was 6845 kg and control group of cows was 6763 kg. Average number of parturition in I group was 2.65 and 2.78 in control group. Average days open in the group I were 118.20 while in control group of cows were longer (p<0, 05) 131.11 days. All services conception rate was lower (p<0.05) in group treated with ozone (1.78) than in control group (2.13). Cows treated with Riger spray became pregnant earlier and had better CR. A conclusion may be made that preventive intrauterine application of ozone (spray), improved the reproductive efficacy in dairy cows.
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In order to optimise reproductive performance and thus production, it is now impossible to control the reproduction of dairy cows without the use of hormones. Due to the characteristics of dairy cows, the use of hormones not only reduces... more
In order to optimise reproductive performance and thus production, it is now impossible to control the reproduction of dairy cows without the use of hormones. Due to the characteristics of dairy cows, the use of hormones not only reduces the need for visual heat detection and thus the number of undetected cows in heat, but also prevents certain problems associated with intensive production and its negative effects on reproduction. Ovsynch, as a planned combination of GnRH and PGF2α that allows artificial insemination at the optimal time without the need to control ovaries and uterus, once offered solutions to these problems, but over time its shortcomings were recognised. Therefore, pre-synchronisation protocols have been developed to create optimal conditions for Ovsynch and allow for the best outcome. In addition to pre-synchronisation, Ovsynch and its modifications can also be used in the resynchronisation of inseminated and sonographically diagnosed non-pregnant cows as soon as ...
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Objectives. The aim of this study was to investigate the lambing distribution of the Romanov breed in northwestern Croatia during five consecutive years. Most European breeds of sheep are seasonal breeders in the moderate climate region ;... more
Objectives. The aim of this study was to investigate the lambing distribution of the Romanov breed in northwestern Croatia during five consecutive years. Most European breeds of sheep are seasonal breeders in the moderate climate region ; however, the Romanov breed is a meat breed that is aseasonally polyoestrous. Romanov sheep are considered to be highly fertile, with a fecundity rate of 230% or more. In Croatia, the lambing season is primarily in the winter and spring for seasonal breeders. Materials and methods. During five consecutive years (December 2011 to November 2016) at eight medium-scale sheep farms in northwestern Croatia, there were 5379 mattings with 5046 successful conceptions i.e. lambings. Ewes were kept at pasture, which primarily provided area for exercise, with access to stables during the night, in a semi-intensive environment. According to standard farming practices, animals had free access to good quality meadow hay (about 1.8 kg per doe daily), adequate concentrate and drinking water. Results. Fertility was 93.81%. The seasonal distribution of lambings in this study was: 47.64% of ewes delivered in winter (n=2422), 23.37% in spring (n=1179), 18.82% in summer (n=950) and 9.81% in autumn (n=495). The winter season refers to the period of December to February. Sexual activity was lowest was during spring and early summer (March to June) with a peak of sexual activity from August to October. Litter size was greater during spring and winter than in other seasons (1.67 vs.1.36) though birth weight was lower in larger than in smaller litters (2.64±0.65 vs. 2.87±0.61). Conclusions. More lambs during lambing season and a higher percentage of multiple births (triplets, quadruplets, etc.) was expected during the optimal breeding season, as seen in most European sheep breeds. Despite being aseasonally polyoestrous, the distribution of mating and lambing was not uniform through the seasons for the Romanov breed.
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This article examines the technique of in vivo production of goat embryos, superovulatory response, embryo recovery of donor does as well as the obstacles associated with those procedures (early luteolysis, anti pFSH antibodies, growths).... more
This article examines the technique of in vivo production of goat embryos, superovulatory response, embryo recovery of donor does as well as the obstacles associated with those procedures (early luteolysis, anti pFSH antibodies, growths). For that reason does are treated by inserting the vaginal sponges for 11 days and a pFSH treatement for three days in decreasing doses, beggining on the 9th day. 24 hours after removing the sponges, does are hand-mated. 7 day later, medioventral laparotomy is performed and the basis and tip of the horn are punctured. The lavage of each horn is performed by retrograde perfusion. Our research was made on 3 boer race goats. Goats responded with 16 ovulations on average. One of three goats has a early luteolysed CL, and even though the sponge is applied 3 days after mating, the phenomenon wasn't minimized. The lavage of uterus resulted with 4 retarded morulas and 15 unfertilized oocytes. The other 2 goats had 10 and 17 CL respectively, from which 2...
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The objective of the present study was to compare transrectal ultrasonography and pregnancy associated glycoprotein (PAG) tests for determination of pregnancy in Holstein-friesian heifers imported in Croatia. 72 heifers imported from... more
The objective of the present study was to compare transrectal ultrasonography and pregnancy associated glycoprotein (PAG) tests for determination of pregnancy in Holstein-friesian heifers imported in Croatia. 72 heifers imported from Holland were used in present study. All came to Croatia with written confirmation of pregnancy. During caranteen we preformed rectal and ultrasound exploration of heifers and simultaneusly collected blood samples from each heifer. PAG concentrations were determined by a RIA imunoassay using bovine PAG 67 KDa as a tracer and standard. In our study 68 hefers confirmed to be pregnant by both methods and 4 cows were confirmed for abortion by PAG test and nonpregnant for ultrasound checking. Transrectal ultrasonography is accurate test for pregnancy diagnosis in pregnant heifers but it is impossible to say only on basis of ultrasound examination if heifers conceived and aborted or they failed to conceive in the first place. PAG RIA test is not only highly ac...
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The influence of milk production on return of ovarian cyclicity in puerperium of Simmental cows was the aim of research. It included the total of 25 cows at the ages of 2 to 10 years. We determined following metabolic parameters: glucose,... more
The influence of milk production on return of ovarian cyclicity in puerperium of Simmental cows was the aim of research. It included the total of 25 cows at the ages of 2 to 10 years. We determined following metabolic parameters: glucose, total proteins, urea, AST, ALT and creatine kinase and following hormones: progesterone P4 and IGF-I. Cows were divided into two groups and examined gynaecological and by ultrasound. In the first group of 13 cows the milk production was 25 L. We took blood samples twice weekly from 4th till 45th day of puerperium. We determined significantly higher level (P 0, 05) between the groups in the level of proteins, urea, glucose and creatine kinase. The group of cows with higher milk production the level of progesterone was 8, 81± ; ; 1, 52 nmol/L, and the group of cows with lower milk production it was 10, 82± ; ; 2, 23 nmol/L. The group of cows with higher milk production the level of IGF-I was 75, 06± ; ; 3, 28 ng/mL, and in the group of cows with lowe...
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The objective of this study was to determine the reproductive performance in Boer goats under semi- intensive management in north-western Croatia over three consecutive years. Sixty Boer does, aged 2 to 6 years, were divided into three... more
The objective of this study was to determine the reproductive performance in Boer goats under semi- intensive management in north-western Croatia over three consecutive years. Sixty Boer does, aged 2 to 6 years, were divided into three groups. A total of 1.80 kids were born per doe (435 newborn in 242 pregnancies). Boer goat fertility was 93.44%. Does with single kids accounted for 22.53% (n = 98), with twins 49.20% (n = 107), triplets 18.62% (n = 27), quadruplets 7.35% (n = 8) and quintuplets 2.29% (n = 2). The average birth weight of Boer kids was 3.48 ± 0.04 kg (1.70 kg to 5.4 kg). The birth weight of goat kids in pluriparous Boer does was signifi cantly (P<0.05) higher in comparison to kids in primiparous does by gender. The birth weight of Boer male goat kids was signifi cantly (P<0.05) higher than female Boer kids. There was no impact from goat age, number of kiddings and birth weight. More than 76% of Boer does delivered in the winter and spring. It is supposed that aft...
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This article examines the procedure of recipient does hormonal preparation and the technique of semilaparoscopic embryotransfer. Furthermore, the direct transfer technique of thawed embryos was discussed, as well as the obstacles... more
This article examines the procedure of recipient does hormonal preparation and the technique of semilaparoscopic embryotransfer. Furthermore, the direct transfer technique of thawed embryos was discussed, as well as the obstacles associated with those procedures which should significantly contribute to genetic improvement of the flocks. The hormonal preparation of recipient does was consisted of insertion of vaginal sponges during 11 days and application of eCG (equine chorionic gonadotrophin) and prostaglandins on day 9. 24h after sponges have been removed, detection of oestrus is performed. Seven days later semilaparoscopic direct transfer of thawed embryos is performed. Before the transfer, uterus, ovaries and the quality of corpora lutea were visually evaluated by laparoscope. There should be at least one functional corpus luteum (even better 2 of them), red in colour and 6-7 days old in order to use the goat as a recipient. Late ovulations as well as early luteolisis should be ...
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Das Ziel vorliegender Studie war die Beobachtung der Fruchtbarkeitsmerkmale der Burenziegen im nordwestlichen Teil Kroatiens, wie Trachtigkeitsdauer, Geburtsgewicht, Zahl der Lammer pro Wurf, Geburtenzahl pro Ziege und Geschlecht der... more
Das Ziel vorliegender Studie war die Beobachtung der Fruchtbarkeitsmerkmale der Burenziegen im nordwestlichen Teil Kroatiens, wie Trachtigkeitsdauer, Geburtsgewicht, Zahl der Lammer pro Wurf, Geburtenzahl pro Ziege und Geschlecht der Lammer sowie ihre wechselseitigen Beziehungen. Die Untersuchung dauerte 5 Jahre und es wurden insgesamt 60 Ziegen aus drei Zuchtgruppen im Alter von zwei bis acht Jahren eingesetzt. Sie wurden auf Intensivweide zusammen mit ihren Lammern gehalten. Die durchschnittliche Trachtigkeitsdauer betrug von 148, 36 ± ; 0, 86 Tage, bzw. von 142 bis 159 Tage. Insgesamt wurden 291 Ablammungen beobachtet, wobei 529 Nachkommen (270 mannliche und 259 weibliche Lammer) geboren wurden. Die Zahl der Lammer pro Wurf betrug von 1 bis 4 (durchschnittlich 1, 82 pro Wurf, bzw. 2, 56 Lammer/Ziege/Jahr). Ein Einzellamm wurde von 32, 98 % Ziegen (n=96) geboren, Zwillinge von 54, 64 % Ziegen (n=159), Drillinge von 9, 97 % Ziegen (n=29), wahrend von 2, 41 % Ziegen (n=7) vier Lamme...
Research Interests: Biology and Gynecology
By this research we aimed to establish how much service period can be shortened in dairy cows by control of puerperium. We have encompassed eighty cows on two mini farms in the Counties of Varaždinska and Međimurska. Forty cows divided in... more
By this research we aimed to establish how much service period can be shortened in dairy cows by control of puerperium. We have encompassed eighty cows on two mini farms in the Counties of Varaždinska and Međimurska. Forty cows divided in two groups were observed on each mini farm. The experimental group of cows on each farm was subject to rectal examination and ultrasound on the 20th and 40th day after calving and immediately treated if where required. We examined and inseminated cows only at the owner’s call without prior controls or therapy. In the Simmental cows, average duration of service period in the control group was 121, 6 ± 8, 83 (M±S.E.M.) days, and conception rate was 2.6. While in the experimental group, service period was 108, 5 ± 6, 12 days and conception rate was 2.4. In the Holstein cows, service period in the control group was 156, 2 ± 12, 45 days and conception rate was 3.3. In the experimental group, service period was 116, 1 ± 8, 47 days, and conception rate wa...
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It is already known that parity could influence pregnancy rate in domestic animals. It is also widespread known that number of kidds could influence level of progesterone during pregnancy in goats and ewes. In this study two groups of... more
It is already known that parity could influence pregnancy rate in domestic animals. It is also widespread known that number of kidds could influence level of progesterone during pregnancy in goats and ewes. In this study two groups of boer goats were compared (10 primiparous goats and 10 pluriparous goats) kept in the same herd and covered by the same buck with proved fertility . The aim of the study was to determine whether parity could influence pregnancy rate and progesterone level. Number of kidds was also observed to exclude influence of kidd number on progesterone level. Blood samples were collected weekly from mating till parturition to determine level of progesterone in both groups. Goats were monitored using ultrasound probes and number of kidds were observed after parturition. Blood was centrifuged and sera samples were stored at -20°C till analysis. Level of progesterone in sera samples was determined using standard RIA method with conjugated steroids (Coat-A-Count TKPG, ...
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Na ergeli engleskih punokrvnjaka "Serdar" u Blatu pored Zagreba redovito su rektalno i ultrazvucno pregledavane kobile od poroda do utvrđene koncepcije 18.-22. dana nakon pripusta. Od ukupno 9 kobila razlicite starosti (od 4 do... more
Na ergeli engleskih punokrvnjaka "Serdar" u Blatu pored Zagreba redovito su rektalno i ultrazvucno pregledavane kobile od poroda do utvrđene koncepcije 18.-22. dana nakon pripusta. Od ukupno 9 kobila razlicite starosti (od 4 do 11 godina) bez ikakvih intervencija je koncipiralo njih 4 odnosno 45% a nakon provedene dijagnostike i terapije jos 3 ili ukupno 7 tj.78%. Od preostale dvije koje nisu koncipirale jednu je bilo nemoguce pregledati, a druga je imala citav niz zdravstvenih problema a malo nakon pripusta je i uginula od kolike usljed zapletaja crijeva. Iako je u nas rad ukljucen relativno mali broj kobila, pa je tesko statisticki obraditi i komentirati rezultate, primjetno je da su reagirale na terapiju sukladno podacima iz literature, a od velike nam je pomoci bilo i konstantno ultrazvucno pracenje promjena na maternici i jajnicima, bez kojeg je tesko ne samo pravilno i ciljano reagirati vec i provjeriti rezultate. U svakom slucaju, sve su kobile s vidljivim žutim tij...
Research Interests: Physics and Humanities
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In the present research the influence of metabolic parameters on return of ovarian cyclicity in puerperium of Holstein-frisian cows was investigated. We determined following metabolic parameters: glucose, total proteins, urea, enzymes... more
In the present research the influence of metabolic parameters on return of ovarian cyclicity in puerperium of Holstein-frisian cows was investigated. We determined following metabolic parameters: glucose, total proteins, urea, enzymes AST, ALT and enzyme creatine kinase in total of 30 cows, during the 45 days of puerperium. Cows between 2 and 4 years old were examined gynecological and with ultrasound. Cows were divided in two groups based on their lactation. In the first group, lactation was 30 L. Blood samples were taken from v. coccigea twice a week starting from day 4 till day 45 of puerperium. Samples were centrifuged and stored frozen on -20°C till further biochemical tests. We determined significant difference (P 30 L had significantly increased AST and ALT levels, in relation to the group of cows with milk production <30 L, and also considering pshysiologic levels. However, creatine kinase levels were in physiologic levels in both groups of cows, and did not significantly...
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Sheep and goat breeding has long tradition in the Balkan countries, making it leading trade in animal husbandry. Dynamic changes in global agriculture production from traditional to industrial livestock technologies, has also impacted the... more
Sheep and goat breeding has long tradition in the Balkan countries, making it leading trade in animal husbandry. Dynamic changes in global agriculture production from traditional to industrial livestock technologies, has also impacted the small ruminant farming systems. The reproduction management is considered to be a crucial point for good farming practice among other animal husbandry factors (housing, nutrition, selection, healthcare, etc.). In the last few decades, our sheep and goat farming systems have introduced various assisted reproduction techniques, such as estrous and ovulation synchronization, laparoscopic intrauterine insemination, embryo production (MOET and IVF), semen cryoconservation, photoperiod manipulation etc. This article reviews the current novelties in this field, presenting worldwide scientific reports and our personal experiences in research and translation to everyday farm practice. Ovine and caprine species have been considered as typical seasonal breede...
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ContentsThe aim of this study was to investigate the effects of exogenous melatonin on libido and semen quality parameters in bucks during the non‐breeding season. Twelve bucks of the French alpine breed from 1.5 to 4 years of age were... more
ContentsThe aim of this study was to investigate the effects of exogenous melatonin on libido and semen quality parameters in bucks during the non‐breeding season. Twelve bucks of the French alpine breed from 1.5 to 4 years of age were assigned into melatonin (MG) and control (CG) groups, with 6 bucks in each group. The experimental period was 3 months (March–May), divided into six periods of 15 days each. The bucks in the MG group received four melatonin implants at the end of March. Two semen samples were taken from the bucks by artificial vagina once per week and their libido estimated. Volume and spermatozoa concentration, their mass motility and motility, proportion of live and total abnormal and forms with abnormal head and tail were determined in the obtained ejaculate samples. The total number of spermatozoa and functional spermatozoa fraction in the ejaculate was also calculated. The MG bucks had significantly higher mass motility and motility of spermatozoa in the first ha...
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INDI_SHEEP TRADI_CHEESE predstavlja europski istraživacki projekt kofinanciran kroz okvirni program FP7 (SEE-ERA.NETPlus). Radni paketi posloženi su kako slijedi: 1. OSIGURANJE OPSKRBE MLIJEKOM AUTOHTONIH PASMINA U ovom radnom paketu... more
INDI_SHEEP TRADI_CHEESE predstavlja europski istraživacki projekt kofinanciran kroz okvirni program FP7 (SEE-ERA.NETPlus). Radni paketi posloženi su kako slijedi: 1. OSIGURANJE OPSKRBE MLIJEKOM AUTOHTONIH PASMINA U ovom radnom paketu analizirat ce se reproduktivne karakteristike i osobitosti nekih lokalnih pasmina ovaca (ovcepoljska pramenka u Makedoniji, dalmatinska i licka pramenka u Hrvatskoj, sjenicka pramenka u Srbiji i lezbo iz Grcke) od cijeg se mlijeka proizvode tradicionalni sirevi u svrhu dobivanja uvida o mogucnostima povecanja produktivnosti autohtonih pasmina ovaca. Uvid ce se dobiti detaljnom analizom reproduktivnih svojstava i osobitosti na osnovu kojih ce biti moguce stvoriti unaprijeđene reproduktivne programe kojima ce se moci ostvariti povecana produktivnost stada, ukoliko potrebu za povecanjem produktivnosti stada izraze ovcari ili njihove udruge. Pristup I: Ovaj pristup orijentiran je primjeni metoda potpomognute reprodukcije u radu s autohtonim pasminama ovaca....
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Ženke tvora su sezonski poliestrične. Hormon melatonin regulira početak rasplodne sezone u ožujku i završetak u kolovozu kada se dnevna svjetlost počinje skraćivati. Prvi znak estrusa u ženke je otečena stidnica ružičaste boje iz koje se... more
Ženke tvora su sezonski poliestrične. Hormon melatonin regulira početak rasplodne sezone u ožujku i završetak u kolovozu kada se dnevna svjetlost počinje skraćivati. Prvi znak estrusa u ženke je otečena stidnica ružičaste boje iz koje se cijedi viskozan iscjedak. Budući da najveći broj folikula na jajnicima dozrije 10- 14 dana od početka estrusa tada je indicirano parenje. Ovulacija je inducirana kopulacijom i nastupa nakon 30-40 sati. Osim kopulacije u indukciji ovulacije se mogu primijenjivati hormonske injekcije hCG ili GnRH 10 ili više dana od početka estrusa. Za primjenu hormonskih injekcija estrogen ne smije biti povišen dulje od 4 tjedna. Estrus se može prekinuti i ovarijohisterektomijom u početku estrusa dok visoke razine estrogena ne počnu toksično djelovati na stanice koštane srži. Ukoliko se estrus ne prekine ni jednom od spomenutih metoda, folikuli i jajne stanice će atrezirati, a koncentracija estrogena će ostati visoka (hiper- estrogenizam). Posljedica toksičnog djelov...
Primjenom metoda kontrolirane reprodukcije, početkom rasplođivanja, poboljšava se plodnost visokoproizvodnih mliječih krava. Unatoč preciznoj tehnologiji i dugotrajnom iskustvu u primjeni indukcijsko-sinkronizacijskih protokola, malo se... more
Primjenom metoda kontrolirane reprodukcije, početkom rasplođivanja, poboljšava se plodnost visokoproizvodnih mliječih krava. Unatoč preciznoj tehnologiji i dugotrajnom iskustvu u primjeni indukcijsko-sinkronizacijskih protokola, malo se zna o osobinama LH pulsacijskih valova. Cilj istraživanja bio je ustanoviti svojstva predovulacijskih LH valova u krava podvrgnutih različitim protokolima s umjetnim osjemenjivanjem u točno određeno vrijeme (UO). Korišteni su arhivski podaci iz istraživanja provedenog na 60 krava holštajnsko-frizijske pasmine, u ranoj laktaciji, podijeljenih u tri skupine: skupina A podvrgnuta je modificiranom Double Ovsynchu (DO), skupina B modificiranom Presynchu (PS), dok je skupina C podvrgnuta Ovsynch (OV) protokolu. Životinjama su uzimani uzorci krvi za određivanje LH (n = 15) i progesterona (n = 60). Sve krave umjetno su osjemenjene 6 i 24 sata nakon druge injekcije GnRH. U krava skupine B postotak steonih krava 30 dana poslije umjetnog osjemenjivanja (UO) bio...
Cilj našeg istraživanja bio je određivanje fizioloških posebnosti spolnog ciklusa i rasplodne sezone ovaca pasmine lička pramenka. U tu svrhu uzorkovana je krv ovcama i ovnu te rađena orhidometrija ovna u periodu od 10. 6. 2011. do 05.... more
Cilj našeg istraživanja bio je određivanje fizioloških posebnosti spolnog ciklusa i rasplodne sezone ovaca pasmine lička pramenka. U tu svrhu uzorkovana je krv ovcama i ovnu te rađena orhidometrija ovna u periodu od 10. 6. 2011. do 05. 09. 2012. godine radi utvrđivanja sezonosti. U periodu od 10.–31. listopada 2012. godine uzorkovana je krv ovaca s ciljem određivanja duljine trajanja spolnoga ciklusa s time da je estrus ovaca prethodno sinkroniziran dvokratnom i.m. aplikacijom (u razmaku od 12 dana) sintetičkog analoga prostaglandina F2α (Estrumate®, cloprostenol) u dozi od 75 μg. Koncentracija progesterona i testosterona u serumu je analizirana pomoću automatskog sistema Immulite® One. Analiza LH rađena je pomoću Imunoenzimnog testa (ELISA). Statistička analiza podataka načinjena je pomoću programskog paketa SAS (Statistical Analysis Software) 9.1.3. Na osnovu dobivenih podataka o koncentraciji progesterona zaključili smo da sezona spolne aktivnosti započinje krajem srpnja. Većina ...
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Plemenita pečurka (Agaricus bisporus), osim što je zdrava hrana, pripada i skupini funkcionalne hrane što potiče daljnja istra-živanja njezine primjene kao funkcionalne hrane, odnosno funkcionalnog dodatka hrani ljudima, a i za domaće... more
Plemenita pečurka (Agaricus bisporus), osim što je zdrava hrana, pripada i skupini funkcionalne hrane što potiče daljnja istra-živanja njezine primjene kao funkcionalne hrane, odnosno funkcionalnog dodatka hrani ljudima, a i za domaće životinje namijenjene ljudskoj prehrani. Zbog prisutnosti nekih bi¬oaktivnih sastojaka iz skupine polisaharida, lipopolisaharida, esencijalnih aminokiselina, peptida, glikoproteina, nukleozida, triterpe¬noida, lektina, masnih kiselina i njihovih de¬rivata, za ove gljive poznati su protuupalni, protuvirusni, protubakterijski, hepatoprotek¬tivni, protudijabetički, hipolipemijski, pro-tutrombotski, hipotenzivni učinci i sinbiotska svojstva (neizravni probiotik/izravni prebio¬tik). U ovom istraživanju opisane su pozitivne naznake smanjenja lipida i povećanja aktiv¬nosti enzima u m. semimembrannosus janjadi hranjene svježom plemenitom pečurkom, što upućuje na bolji metabolizam i kvalitetu mesa. Na temelju dosadašnjih istraživanja u sklopu projekta HRZZ-a In...
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1Department of Pathophysiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia 2Clinic for Obstetrics and reproduction, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia 3Veterinary Practice... more
1Department of Pathophysiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia 2Clinic for Obstetrics and reproduction, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia 3Veterinary Practice Đurđevac, Đurđevac, Croatia 4Department of Medical Biochemistry and Laboratory Medicine, Merkur University hospital, Zagreb, Croatia 5Department for nutrition and animal husbandry, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Research Interests: Biology and Paraoxonase
Dražen ĐURIČIĆ, DVM, PhD, Assistant Professor, Veterinary Practice Đurđevac, Đurđevac, Croatia; Miroslav BENIĆ, DVM, PhD, Assistant Professor, Luka CVETNIĆ, DVM, Assistant, Croatian Veterinary Institute, Zagreb, Croatia; Nino MAĆEŠIĆ,... more
Dražen ĐURIČIĆ, DVM, PhD, Assistant Professor, Veterinary Practice Đurđevac, Đurđevac, Croatia; Miroslav BENIĆ, DVM, PhD, Assistant Professor, Luka CVETNIĆ, DVM, Assistant, Croatian Veterinary Institute, Zagreb, Croatia; Nino MAĆEŠIĆ, DVM, PhD, Assistant Professor, Hrvoje VALPOTIĆ, DVM, PhD, Assistant Professor, Romana TURK, Mag. Med. Biochem., PhD, Associate Professor, Vesna DOBRANIĆ, DVM, PhD, Associate Professor, Damjan GRAČNER, DVM, PhD, Full Professor, Silvijo VINCE, DVM, PhD, Assistant Professor, Juraj GRIZELJ, DVM, PhD, Associate Professor, Martina LOJKIĆ, DVM, PhD, Associate Professor, Marko SAMARDŽIJA*, DVM, PhD, Full Professor, (corresponding author, e-mail: smarko@vef.hr), Faculty of Veterinary Medicine University of Zagreb, Croatia; Jože STARIČ, DVM, PhD, Assistant Professor, Faculty of Veterinary Medicine University of Ljubljana, Slovenia Introduction
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Late pregnancy and early lactation is a critical period for health and productivity of dairy cows due to the energy deficit with a high incidence of metabolic diseases and reduced fertility. The objective of this study was to investigate... more
Late pregnancy and early lactation is a critical period for health and productivity of dairy cows due to the energy deficit with a high incidence of metabolic diseases and reduced fertility. The objective of this study was to investigate the relationship between parameters of lipid mobilisation and paraoxonase-1 (PON1) activity during the transition period. The study was conducted on 24 Holstein-Friesian dairy cows aged 2-7 years. Blood samples were taken on days -30, -10, -2, 0, 5, 12, 19, 26 and 60 relative to parturition. Serum triglycerides (TG), total cholesterol (TC), HDL-cholesterol (HDL-C), nonesterified fatty acids (NEFA) and β- hydroxybutirate (BHB) concentrations were assayed by the standard commercial kits. The PON1 activity was measured spectrometrically by the method of hydrolysis of paraoxon. Serum NEFA concentration was significantly elevated at calving and stayed at the highest values up to day 19 after calving (P<0.05). As a consequence, BHB concentration increa...
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The aim of this research was to determine etiology and prevalence of udder infection in dairy cows before drying off on dairy farms in Croatia. A research was carried out on 6 dairy farms in Croatia. Milk samples were taken into sterile... more
The aim of this research was to determine etiology and prevalence of udder infection in dairy cows before drying off on dairy farms in Croatia. A research was carried out on 6 dairy farms in Croatia. Milk samples were taken into sterile plastic tubes during the last milking prior to drying off. Bacteriology examination of milk samples had obtained 54 positive samples ; prevalence of intramammary infection (IMI) was 22.5%. The most common pathogens of IMI prior to drying off were Streptococcus spp. Lancefield group D (7.79%) and Staphylococcus aureus (6.56%). Other pathogens were coagulase-negative staphylococci (2.87%), Pseudomonas spp. (2.46%), Streptococcus agalactiae (2.05%), and other microorganisms (1.23%). In this research higher IMI prevalence in rear quarters (P= 0.03) in comparison to front quarters was observed. There were no significant differences in infection prevalence (P=0.07) comparing right to left quarters. Significantly higher prevalence of infection caused by Str...
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The aim of present study was to study the effect of parity and fecundity on the body condition score and blood serum concentration of metabolites (beta-hydroxybutyrate, glucose, total cholesterol and triglycerides) during the pre and post... more
The aim of present study was to study the effect of parity and fecundity on the body condition score and blood serum concentration of metabolites (beta-hydroxybutyrate, glucose, total cholesterol and triglycerides) during the pre and post parturient period in German Improved Fawn goats. Forty goats (primiparous (n = 18) and multiparous (n = 22) were used in the study. Blood sampling were done 4 times for each goat, starting 20 days before expected parturition, after parturition, and on the 20th and 40th day of lactation. Blood samples were collected by jugular puncture. In the obtained blood serum concentrations of metabolites were determined by the standard method of absorptive spectrophotometry. The total concentration of beta-hydroxybutyrate and triglycerides in goat blood serum was not different within the category of body condition score, but the level of glucose was signifi cantly lower in intermediate and fat goats and the level of total cholesterol was higher (P<0.05) in ...
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Research Interests: Reproduction and Biology
A 1.5-year-old mongrel bitch was presented at the Clinic for obstetrics and reproduction at the Faculty of Veterinary Medicine in Zagreb for spaying. Since the animal had been found and kept in animal shelter, no anamnestic data about her... more
A 1.5-year-old mongrel bitch was presented at the Clinic for obstetrics and reproduction at the Faculty of Veterinary Medicine in Zagreb for spaying. Since the animal had been found and kept in animal shelter, no anamnestic data about her reproductive status were known. A general examination did not reveal any preoperative problem. On ultrasonograph it was found that bitch was about four weeks pregnant. Finally, and with the owner’s consent, ovariohysterectomy was proposed and accepted as a radical alternative to medical abortion following an unwanted pregnancy, as it also involves defi nitive sterilization. The surgical procedure comprising ovariohisterectomy was performed under general endotracheal anesthesia. The procedure revealed segmental aplasia of the left uterine horn with an intact left ovary and kidney.
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Monika JUKIĆ, dr. med. vet., Hrvatska; dr. sc. Ivona ŽURA ŽAJA, dr. med. vet., postdoktorandica, dr. sc. Juraj GRIZELJ, dr. med. vet., izvanredni profesor, dr. sc. Marko SAMARDŽIJA, dr. med. vet., redoviti profesor, dr. sc. Silvijo... more
Monika JUKIĆ, dr. med. vet., Hrvatska; dr. sc. Ivona ŽURA ŽAJA, dr. med. vet., postdoktorandica, dr. sc. Juraj GRIZELJ, dr. med. vet., izvanredni profesor, dr. sc. Marko SAMARDŽIJA, dr. med. vet., redoviti profesor, dr. sc. Silvijo VINCE*, dr. med. vet., docent (dopisni autor, e-mail: svince@vef.hr), Veterinarski fakultet Sveučilišta u Zagrebu, Hrvatska; dr. sc. Dražen ĐURIČIĆ, dr. med. vet., znanstveni savjetnik, Veterinarska stanica Đurđevac, Hrvatska Uvod
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Data concerning the morphometric parameters of sheep red blood cells (RBCs) obtained using computer-assisted image analysis have not yet been investigated, and there are no data on any analyses of ovine RBC subpopulations based on their... more
Data concerning the morphometric parameters of sheep red blood cells (RBCs) obtained using computer-assisted image analysis have not yet been investigated, and there are no data on any analyses of ovine RBC subpopulations based on their morphometric parameters. The aims of this study are to determine the values of RBC haematological and morphometric size and shape parameters, to form groups according to the obtained values of haematological parameters; to determine the differences in RBC morphometric parameters between the formed groups, and to determine RBC subpopulations and their respective proportions in the formed groups. Thirty-six blood samples were collected from the jugular vein of clinically healthy Lika pramenka sheep, aged between 2 and 5 years. Haematological parameters including haemoglobin (HGB), haematocrit (HTC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), and RBC distribution width were analy...
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Dans les élevages ovins et caprins, la maîtrise de la reproduction est importante pour une reproduction hors saison sexuelle, pour grouper les mises bas et pour la pratique de l’Insémination Artificielle (IA). Le traitement hormonal... more
Dans les élevages ovins et caprins, la maîtrise de la reproduction est importante pour une reproduction hors saison sexuelle, pour grouper les mises bas et pour la pratique de l’Insémination Artificielle (IA). Le traitement hormonal d’induction et de synchronisation de l’œstrus et de l’ovulation est aujourd’hui le moyen le plus efficace pour atteindre ces objectifs. Le recours à des traitements photopériodiques et/ou à l’effet mâle apparait comme une solution pour limiter l’utilisation d’hormones dans ces filières.Cet article rappelle les spécificités de la réponse ovulatoire et comportementale des femelles à « l’effet mâle », ainsi que les bases physiologiques qui contribuent à la réussite de cette pratique chez les ovins et les caprins. Le prétraitement des animaux avec des traitements photopériodiques de désaisonnement est un prérequis pour une réponse à l’effet mâle efficace, notamment chez des races très saisonnées comme les races caprines alpine et saanen. Des traitements lumi...
Research Interests: Humanities and Biology
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The aim of the study was to establish the influence of lactation yield level on reproductive performance of cows in the Puerperium. The research included 90 cows Simmenthal breed during Puerperium. The animals were selected by their milk... more
The aim of the study was to establish the influence of lactation yield level on reproductive performance of cows in the Puerperium. The research included 90 cows Simmenthal breed during Puerperium. The animals were selected by their milk yield in the 3rd and 4th lactation and 3 experimental groups were formed. The low production group for values under 3500 L per lactation; the second group for values under 4500 L per lactation, and the high production group for values above 4500 L per lactation. All 3 groups consisted of 30 animals. Besides detailed anamnesis and clinical health status data, the following data were recorded for each animal during the study: puerperal endometritis, retained placenta, ovarian disorders and chronic endometritis during puerperium. Furthermore, the duration of open days period was taken as important factor for evaluation of cow reproductive performance. In conclusion, the high production group had significantly longer open days period, insemination index...
Research Interests: Medicine and Gynecology
de BULNES, T. DOVENSKI, L. TURMALAJ, B. ŽEVRNJA: Use of ultrasonography to detect ovarian response in goats submitted to multiple ovulation and embryo transfer program. Vet. arhiv 83, 125-134, 2013. ABSTRACT The aim of this research was... more
de BULNES, T. DOVENSKI, L. TURMALAJ, B. ŽEVRNJA: Use of ultrasonography to detect ovarian response in goats submitted to multiple ovulation and embryo transfer program. Vet. arhiv 83, 125-134, 2013. ABSTRACT The aim of this research was to establish the importance and accuracy of ultrasonic examination of goats in order to estimate the superovulatory response. For this reason, 28 donor goats were systematically examined by real time ultrasonography to count the preovulatory follicles at observed oestrus onset and to confi rm respective ovulation(s) 48 h later. The accuracy of ultrasound exams was analysed comparing the number of preovulatory follicles with number of corpora lutea (CL′s) counted during embryo collection. The total relative undervalue of ultrasound prediction of multiple ovulation was found to be 19.4%. In all ovaries examined, the total number of preovulatory follicles minus number of unovulated follicles was lower than the number of CL′s. The relationship between th...
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Artificial insemination (AI) as a part of assisted reproductive technologies represents the oldest and most widespread method used to accelerate genetic progress in all domestic animals. After its first implementation in ovine... more
Artificial insemination (AI) as a part of assisted reproductive technologies represents the oldest and most widespread method used to accelerate genetic progress in all domestic animals. After its first implementation in ovine reproduction and almost 80 years afterward, AI is continuously used for improving the genetic merit, utilizing either fresh or short-time chilled semen. Nevertheless, regardless of the semen used for insemination, the conception rate (CR) is still lower in comparison to natural service. At least two factors are commonly thought to limit the success of the AI and reduce the CR: (1) failure of placing the semen directly into the uterus due to the specific anatomic structure of the ewe’s cervix; (2) lower viability of ram spermatozoa during cryopreservation (<30% progressively motile spermatozoa after thawing). This review elaborates on recent studies that aimed to achieve acceptable CR through the implementation of cervical or intrauterine insemination: deep ...
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Dr. sc. Silvijo VINCE*, dr. med. vet., docent (dopisni autor, e-mail: svince@vef.hr), dr. sc. Juraj GRIZELJ, dr. med. vet., izvanredni profesor, dr. sc. Branimira ŠPOLJARIĆ, dr. med. vet., viša asistentica, dr. sc. Hrvoje VALPOTIĆ, dr.... more
Dr. sc. Silvijo VINCE*, dr. med. vet., docent (dopisni autor, e-mail: svince@vef.hr), dr. sc. Juraj GRIZELJ, dr. med. vet., izvanredni profesor, dr. sc. Branimira ŠPOLJARIĆ, dr. med. vet., viša asistentica, dr. sc. Hrvoje VALPOTIĆ, dr. med. vet., docent, dr. sc. Marko SAMARDŽIJA, dr. med. vet., redoviti profesor, Veterinarski fakultet Sveučilišta u Zagrebu, Hrvatska; Maja PLATIŠA, dr. med. vet., Guernsey Islands, Velika Britanija; dr. sc. Dražen ĐURIČIĆ, dr. med. vet., znanstveni savjetnik, Veterinarska stanica Đurđevac, Hrvatska; dr. sc. Foteini SAMARTZI, dr. med. vet., redovita profesorica, Hellenic Agriculture Organization-Demeter, Solun, Grčka; Velimir BERTA, dr. med. vet., Veterinarska stanica Varaždin, Hrvatska; Nikola ROŠIĆ, dr. med. vet., Veterinarska stanica Jastrebarsko, Hrvatska; Boris STOJANOV, dr. med. vet., Veterinarski centar Strumica, Makedonija Uvod